CN110468059A - A kind of liquid aerobic microbiological soil-repairing agent and its production application method - Google Patents
A kind of liquid aerobic microbiological soil-repairing agent and its production application method Download PDFInfo
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- CN110468059A CN110468059A CN201810451731.6A CN201810451731A CN110468059A CN 110468059 A CN110468059 A CN 110468059A CN 201810451731 A CN201810451731 A CN 201810451731A CN 110468059 A CN110468059 A CN 110468059A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Soil Sciences (AREA)
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- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a kind of liquid aerobic microbiological soil-repairing agent and its production application method, soil-repairing agent includes the raw material of following mass fraction proportion: lactic acid bacteria 30%, saccharomycete 20%, photosynthetic bacteria 20% and bacillus 30%；The microbe soil restoration agent prepared by above-mentioned raw materials by fermented and cultured can be suitable for the restoration of the ecosystem of abandoned mine, the reparation of farmland pollution soil, the improvement in salt-soda soil, the reparation of petroleum hydrocarbon contaminated soil, and, raw material are readily available, it is low in cost, later period Engineering operation is easy, and opposite repairing efficiency is short；In addition, the action condition of microorganism is easily controllable, show that pollutant concentration can reduce by 84% in the contaminated soil after handling 6 weeks using this restorative procedure by engineering practice, the concentration of pollutant can reach in the critical field of country's permission after reparation.
Description
Technical field
The present invention relates to field of biotechnology, specially a kind of liquid aerobic microbiological soil-repairing agent and its production are used
Method.
Background technique
All kinds of microbe soil restoration agents are more currently on the market, mostly graininess, and bacterium activity lowers significantly, original used
Material is complicated, is not easy to realize large-scale production and application.
Moreover, existing microbe soil restoration agent fabrication cycle is long, relative cost is higher, and when application wants environmental condition
It asks comparison harsh, is difficult to realize scale application.
Summary of the invention
The purpose of the present invention is to provide it is a kind of it is simple and effective, economical and practical, secondary pollution will not be brought, it is easily operated,
It is capable of the liquid aerobic microbiological soil-repairing agent and its production application method of large-scale application, to solve above-mentioned background technique
The problem of middle proposition.
To achieve the above object, the invention provides the following technical scheme: a kind of liquid aerobic microbiological soil-repairing agent, packet
Include the raw material of following mass fraction proportion: lactic acid bacteria 30%, saccharomycete 20%, photosynthetic bacteria 20% and bacillus 30%.
Preferably, the bacillus includes bacillus licheniformis, in bacillus subtilis and three kinds of bacillus megaterium
At least two.
The present invention also provides a kind of technical solutions are as follows: a kind of production method of liquid aerobic microbiological soil-repairing agent, packet
Include following steps:
S1: respectively by lactic acid bacteria, saccharomycete, photosynthetic bacteria and bacillus corresponding solid medium, culture 72h, as mother
Bacterium；
S2: female bacterium after culture is inoculated into respectively in the fluid nutrient medium that prior agar is made, 37 DEG C of culture 48h of shaking table；
S3: prepare 4 fermentors, fermentation cylinder for fermentation liquid is raw materials used: glucose, grand celebration full-cream sweet milk powder, yeast extract according to
Ratio 250:65:1 adds water to 1t, mixing sterilizing production fermentation medium；
S4: the bacterium after cultivating in step S2 is inoculated into respectively in the fermentation medium of step S3, cultivates 5d；
S5: the microbial bacterial agent after step S4 culture is mixed to get composite liquefied microbe soil according to the ratio of 3:2:2:3
Remediation microbial inoculum.
The present invention also provides another technical solutions are as follows: a kind of application method of liquid aerobic microbiological soil-repairing agent,
Cooperated when the microbe soil restoration agent is repaired using auxiliary agent and reaches better effect；Wherein auxiliary agent includes: rice husk, urine
Element, compound fertilizer, biological activated carbon；Auxiliary agent and microbial reagent are all made of the method for turning over point 3 times and are applied in contaminated soil, can divide
Not Cai Yong original position soil reparation and dystopy soil remediation, construction period be 8 weeks.
Preferably, microorganism used therefor renovation agent in the repair process: rice husk, urea, compound fertilizer, biological activated carbon according to
The ratio of 33:35:15:1:1 respectively add in proportion when engineering starts, after starting by each week.
Preferably, according to the amount method of turning over of 4:3:3 after the rice husk and microorganism renovation agent first mix in proportion
It uniformly mixes in contaminated soil within 1,3 week when engineering starts and after starting.
Preferably, after the urea, compound fertilizer, biological activated carbon mix in proportion, equally with the method for turning over according to 4:3:3
Amount in 2,4,6 weeks after engineering starts uniformly incorporation contaminated soils.
Preferably, described to turn over method, it turns over every 2 weeks once, each ploughed depth reaches 70cm, sampling analysis in 2,4,8 weeks.
Compared with prior art, the beneficial effects of the present invention are:
1, this liquid aerobic microbiological soil-repairing agent and its production application method, raw material are easy to get, are at low cost, product is liquid
State, microbial activity is high, renovation technique is easy to operate, be applicable in the engineering practice of large-scale go, repair after index
It is short to can achieve national standard, repairing efficiency.
2, this liquid aerobic microbiological soil-repairing agent and its production application method, the ecology suitable for abandoned mine are repaired
Multiple, the reparation of farmland pollution soil, the improvement in salt-soda soil, the reparation of petroleum hydrocarbon contaminated soil, it is simple and effective, economical and practical, no
Secondary pollution, easily operated can be brought, is that a kind of microbe soil for capableing of large-scale application repairs medicament and utilizes the medicine
The new method of agent repairing polluted soil.
Specific embodiment
It will be detailed below the embodiment of the present invention, however, the embodiment of the present invention is not limited thereto.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts,
It shall fall within the protection scope of the present invention.
Embodiment 1:
In the embodiment of the present invention: providing a kind of liquid aerobic microbiological soil-repairing agent, the original including following mass fraction proportion
Material: lactic acid bacteria 30%, saccharomycete 20%, photosynthetic bacteria 20% and bacillus 30%, wherein bacillus includes lichens gemma bar
At least two in bacterium, bacillus subtilis and three kinds of bacillus megaterium.
Embodiment 2:
The 1 a kind of liquid aerobic microbiological soil-repairing agent provided based on the above embodiment, is repaired in order to further illustrate the soil
Multiple agent, the present invention also provides the production methods of the soil-repairing agent, comprising the following steps:
Step 1: respectively by lactic acid bacteria, saccharomycete, photosynthetic bacteria and bacillus corresponding solid medium, culture 72h, work
For female bacterium；
Step 2: female bacterium after culture is inoculated into respectively in the fluid nutrient medium that prior agar is made, 37 DEG C of shaking table cultures
48h；
Step 3: preparing 4 fermentors, and fermentation cylinder for fermentation liquid is raw materials used: glucose, grand celebration full-cream sweet milk powder, yeast extract
Proportionally 250:65:1 adds water to 1t, mixing sterilizing production fermentation medium；
Step 4: the bacterium after cultivating in step 2 is inoculated into respectively in the fermentation medium of step 3, cultivates 5d；
Step 5: the microbial bacterial agent after step 4 culture is mixed to get composite liquefied microorganism according to the ratio of 3:2:2:3
Soil remediation microbial inoculum.
Embodiment 3:
2 description based on the above embodiment, the present invention also provides the application method of the soil-repairing agent, microbe soil restoration agent is repaired
Cooperated when multiple using auxiliary agent and reaches better effect；Wherein auxiliary agent includes: rice husk, urea, compound fertilizer, bioactivity
Charcoal；Auxiliary agent and microbial reagent are all made of the method for turning over point 3 times and are applied in contaminated soil, turn over method, turn over every 2 weeks once, often
Secondary ploughed depth reaches 70cm, and original position soil reparation and dystopy soil remediation, engineering can be respectively adopted in sampling analysis in 2,4,8 weeks
Period is 8 weeks.
Wherein, the microorganism used therefor renovation agent in repair process: rice husk, urea, compound fertilizer, biological activated carbon are according to 33:
The ratio of 35:15:1:1 respectively add in proportion when engineering starts, after starting by each week；Rice husk and microorganism renovation agent first press than
1,3 week according to the amount of 4:3:3 with the method for turning over when engineering starts and after starting uniformly mixes contaminated soil to example after mixing
In；After urea, compound fertilizer, biological activated carbon mix in proportion, equally with the method for turning over according to the amount of 4:3:3 after engineering starts
2,4,6 weeks uniformly incorporation contaminated soils in.
In summary: a kind of liquid aerobic microbiological soil-repairing agent provided by the invention and its production application method are fitted
For the restoration of the ecosystem of abandoned mine, the reparation of farmland pollution soil, the improvement in salt-soda soil, the reparation of petroleum hydrocarbon contaminated soil,
Its raw material for being used to prepare microbe soil restoration agent is readily available, low in cost, and later period Engineering operation is easy, opposite to repair
Period is short；Moreover, the action condition of microorganism is easily controllable, obtained by engineering practice after being handled 6 weeks using this restorative procedure
Contaminated soil in pollutant concentration can reduce by 84%, after reparation the concentration of pollutant can reach country permission mark
In quasi- range.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of liquid aerobic microbiological soil-repairing agent, which is characterized in that the raw material including following mass fraction proportion: lactic acid
Bacterium 30%, saccharomycete 20%, photosynthetic bacteria 20% and bacillus 30%.
2. a kind of liquid aerobic microbiological soil-repairing agent according to claim 1, which is characterized in that the bacillus
Including at least two in bacillus licheniformis, bacillus subtilis and three kinds of bacillus megaterium.
3. a kind of production method of liquid aerobic microbiological soil-repairing agent according to claim 1, which is characterized in that packet
Include following steps:
S1: respectively by lactic acid bacteria, saccharomycete, photosynthetic bacteria and bacillus corresponding solid medium, culture 72h, as mother
Bacterium；
S2: female bacterium after culture is inoculated into respectively in the fluid nutrient medium that prior agar is made, 37 DEG C of culture 48h of shaking table；
S3: prepare 4 fermentors, fermentation cylinder for fermentation liquid is raw materials used: glucose, grand celebration full-cream sweet milk powder, yeast extract according to
Ratio 250:65:1 adds water to 1t, mixing sterilizing production fermentation medium；
S4: the bacterium after cultivating in step S2 is inoculated into respectively in the fermentation medium of step S3, cultivates 5d；
S5: the microbial bacterial agent after step S4 culture is mixed to get composite liquefied microbe soil according to the ratio of 3:2:2:3
Remediation microbial inoculum.
4. a kind of application method of liquid aerobic microbiological soil-repairing agent according to claim 3, which is characterized in that institute
It states to be cooperated when microbe soil restoration agent is repaired using auxiliary agent and reaches better effect；Wherein auxiliary agent includes: rice husk, urine
Element, compound fertilizer, biological activated carbon；Auxiliary agent and microbial reagent are all made of the method for turning over point 3 times and are applied in contaminated soil, can divide
Not Cai Yong original position soil reparation and dystopy soil remediation, construction period be 8 weeks.
5. a kind of application method of liquid aerobic microbiological soil-repairing agent according to claim 4, which is characterized in that institute
State microorganism used therefor renovation agent in repair process: rice husk, urea, compound fertilizer, biological activated carbon according to 33:35:15:1:1 ratio
Example respectively add in proportion when engineering starts, after starting by each week.
6. a kind of application method of liquid aerobic microbiological soil-repairing agent according to claim 4, which is characterized in that institute
State rice husk and microorganism renovation agent first mix in proportion after according to the amount of 4:3:3 with the method for turning over when engineering starts and beginning
1,3 week afterwards is uniformly in incorporation contaminated soil.
7. a kind of application method of liquid aerobic microbiological soil-repairing agent according to claim 4, which is characterized in that institute
State after urea, compound fertilizer, biological activated carbon mix in proportion, equally with the method for turning over according to the amount of 4:3:3 after engineering starts
2,4,6 weeks uniformly incorporation contaminated soils in.
8. a kind of application method of liquid aerobic microbiological soil-repairing agent according to claim 4, which is characterized in that institute
The method of turning over is stated, is turned over every 2 weeks once, each ploughed depth reaches 70cm, sampling analysis in 2,4,8 weeks.
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| CN201810451731.6A CN110468059A (en) | 2018-05-12 | 2018-05-12 | A kind of liquid aerobic microbiological soil-repairing agent and its production application method |
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| CN201810451731.6A CN110468059A (en) | 2018-05-12 | 2018-05-12 | A kind of liquid aerobic microbiological soil-repairing agent and its production application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220234087A1 (en) * | 2021-01-25 | 2022-07-28 | Ming Chi University Of Technology | Method for improving the efficiency of electrokinetic technology for removing pollutants from solid matrix by using a mixed microbial solution |
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| CN107828696A (en) * | 2017-11-30 | 2018-03-23 | 盖萌 | A kind of composite fermentation microbe soil conditioner and preparation method thereof |
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| CN101108779A (en) * | 2007-06-20 | 2008-01-23 | 福建省诏安县绿洲生化有限公司 | Method of manufacturing organic, inorganic compound fertilizer with molasses zymolysis waste liquor and lime sludge |
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| US20220234087A1 (en) * | 2021-01-25 | 2022-07-28 | Ming Chi University Of Technology | Method for improving the efficiency of electrokinetic technology for removing pollutants from solid matrix by using a mixed microbial solution |
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Application publication date: 20191119 |