CN102154106A - Simple culture method for effective microorganisms (EM) - Google Patents
Simple culture method for effective microorganisms (EM) Download PDFInfo
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- CN102154106A CN102154106A CN 201010618454 CN201010618454A CN102154106A CN 102154106 A CN102154106 A CN 102154106A CN 201010618454 CN201010618454 CN 201010618454 CN 201010618454 A CN201010618454 A CN 201010618454A CN 102154106 A CN102154106 A CN 102154106A
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Abstract
The invention relates to a culture method of mixed bacteria, in particular to a culture method for EM. The simple culture method for EM comprises the following steps: A, preparing culture solution, and filling into a plastic square barrel with a ventilating cover; B, inoculating bacillus; C, culturing under a closed condition for 24 hours; D, inoculating composite lactic acid bacteria, saccharomycetes and photosynthetic bacteria; and E, culturing under a closed condition. In the invention, a cheap plastic square barrel which is common on market is used as an EM culture container; generally, culture can be performed at normal temperature; culture and use on site can be realize; and the method has the advantages of environment protection, flexible production operation, production and use cost conservation and the like.
Description
Technical field
The present invention relates to a kind of cultural method of mixed bacterium, relate in particular to the cultural method of a kind of EM bacterium.
Background technology
The EM bacterium, its English name is " Effective Miero-origanisms ", it is to be selected from more than 10 of photosynthetic bacteria, actinomycetes, yeast, compound lactobacillus, genus bacillus etc. to belong to more than the 80 kind of active bacteria formulation that the compound cultivation of microorganism forms, the mutual symbiosis of various microorganisms, interaction in the EM microbial inoculum, common development, give play to multiple function, can promote organic substance decomposition such as animals and plants residue rapidly, suppressed spoilage organism and other pathogen growth; In water ecological environment, the EM microbial inoculum can effectively suppress corrupt biological growth and some growth of pathogenic bacteria, make repugnant substances such as containing ammonia thing and sulfide be difficult for forming, and quicken the decomposition of organic repugnant substance, the water body dissolved oxygen coefficient is improved greatly, thereby can eliminate COD, ammonia nitrogen, the total phosphorus of contaminated water body effectively, degraded is organic.The EM bacterium all has high use value in industry, agricultural and pharmaceutical industries, has also obtained general application.The development requirement of EM bacterium the prospect that has a very wide range of applications, but existing method of producing the EM bacterium have urgent demand to EM bacterium large-scale method for producing low-cost and that be convenient to operate in the part that all haves much room for improvement, industry on production cost and production technique.Because EM microbial inoculum product major part is a liquid state, also has inconvenience thus aspect transportation,, also be the condition of providing convenience of promoting the use of of EM bacterium as if cultivating on the spot and using and to save a large sum of transportation cost.
Summary of the invention
At foregoing, the simple and easy cultural method of EM bacterium that the purpose of this invention is to provide a kind of low cost, low investment and be convenient to operate effectively reduces production cost and the use cost of EM bacterium.
For achieving the above object, the present invention takes following technical scheme:
The simple and easy cultural method of a kind of EM bacterium, following steps:
In A, preparation nutrient solution, the plastic square bucket of joining ventilating cover of packing into;
B, inoculation genus bacillus;
C, airtight cultivation 24 hours;
D, inoculation compound lactobacillus, yeast, photosynthetic bacteria;
E, airtight cultivation.
Nutrient solution in the described steps A contains the composition of following weight part: 6% molasses water, 0.5% peptone.
The plastic square staving of joining ventilating cover in the described steps A is long-pending to be 20-25L.
The inoculum size of genus bacillus is 5% of a nutrient solution weight among the described step B, and the inoculum size of compound lactobacillus, yeast, photosynthetic bacteria is respectively 6%, 5%, 4% of nutrient solution weight among the step D.
The culture temperature of described step C and step e is 20-35 ℃, and preferable culture temperature is 25-35 ℃.
The time that described step e is cultivated is 3-7 days, and preferable incubation time is 5 days.
The present invention adopts the culture vessel of common on the market and cheap plastic square bucket as the EM bacterium, can cultivate under the general normal temperature, can save the cost of investment of expensive fermentor tank aspect investment in production equipment.The present invention adopts 20-25L to join the plastic square bucket of ventilating cover, bottled with plant the cultivation of once finishing the EM bacterium, such method is compared with general fermentor cultivation, the situation that smell scatters when saving packing is impacted less to production environment; Simultaneously, because that 20-25L joins the tunable performance of plastic square bucket of ventilating cover is better, and the production operation step is less, can effectively reduce the probability of microbiological contamination.The plastic square bucket of joining ventilating cover with 20-25L, can effectively solve the problem of aerogenesis in the EM bacterium culturing process, the distortion that plastic tank produced is less, also can effectively weaken in the culturing process a large amount of discharge of gas and influences sealing effectiveness, portably use simultaneously all more convenient, easily operation.
Beneficial effect of the present invention is: 1, more convenient, efficient to the processing of nutrient solution, removed from and adopted a large amount of losses of high-temperature sterilization electric power, also save simultaneously the spending of buying large-scale pressure sterilization pot, only needed a water purifying plant just can reach corresponding effects, reduced cost of investment; As long as 2 have abundant plastic square bucket, just can measure flexibly according to demand and arrange production, to realize cultivating on the spot, in-field use is invested lower; 3, cultured EM bacterium need not transducer package again, and the plastic square bucket that 20-25L joins ventilating cover itself is exactly reasonable packaging vessel, and is both beautiful and practical, and only need sticking corresponding label, promptly to can be used as finished product for sale; 4, the used 20-25L of the present invention joins the plastic square bucket of ventilating cover, can recycle and reuse, and reduces cost; 5, adopt 20-25L to join the plastic square bucket barreled of ventilating cover and once plant, reduced frequency of exposure and the time of EM bacterium and outside air in the production operation process, can reduce the probability of living contaminants.In sum, the simple and easy cultural method of EM bacterium that the present invention provided, the plastic square bucket that adopts 20-25L to join ventilating cover is cultivated, and is easy and simple to handle, simultaneously can realize cultivating in-field use on the spot, have environmental protection, production operation flexibly and save and produce and advantages such as use cost.
Embodiment
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1
1.1 configuration nutrient solution
20L being joined the apparatus such as plastic square bucket, graduated cylinder of ventilating cover handles through sterilization.
Taking by weighing weight ratio respectively is 6% molasses water, 0.5% peptone, adds pure water, and the configuration total amount is the nutrient solution of 20kg.
1.2 inoculation genus bacillus
Take by weighing 1kg genus bacillus (available from Chang Ansheng thing company limited), the bacterial classification that takes by weighing is put in the nutrient solution.
1.3 postvaccinal transparent plastics bucket is descended airtight cultivation 24 hours at 20-35 ℃.
1.4 the inoculation of compound lactobacillus, yeast, photosynthetic bacteria
Take by weighing 1.2kg compound lactobacillus (available from bright Milk Products Plant), 1.0kg yeast (available from Angel Yeast stock company), 0.8kg photosynthetic bacteria (available from Chang Ansheng thing company limited) respectively, the bacterial classification that takes by weighing is put in the nutrient solution.
1.5 with postvaccinal transparent plastics bucket 20-35 ℃ of down airtight cultivation 5 days, cultivate finish after, with general flat band method, the bacterium amount that detects unit volume in the nutrient solution is every milliliter 3,000,000,000 strain.
Embodiment 2
2.1 configuration nutrient solution
25L being joined the apparatus such as plastic square bucket, graduated cylinder of ventilating cover handles through sterilization.
Taking by weighing weight ratio respectively is 6% molasses water, 0.5% peptone, adds pure water, and the configuration total amount is the nutrient solution of 25kg.
2.2 inoculation genus bacillus
Take by weighing 1.25kg genus bacillus (available from Chang Ansheng thing company limited), the bacterial classification that takes by weighing is put in the nutrient solution.
2.3 postvaccinal transparent plastics bucket is descended airtight cultivation 24 hours at 25-35 ℃.
2.4 the inoculation of compound lactobacillus, yeast, photosynthetic bacteria
Take by weighing 1.5kg compound lactobacillus (available from bright Milk Products Plant), 1.25kg yeast (available from Angel Yeast stock company), 1.0kg photosynthetic bacteria (available from Chang Ansheng thing company limited) respectively, the bacterial classification that takes by weighing is put in the nutrient solution.
2.5 with postvaccinal transparent plastics bucket 25-35 ℃ of down airtight cultivation 7 days, cultivate finish after, with general flat band method, the bacterium amount that detects unit volume in the nutrient solution is every milliliter 5,000,000,000 strain.
The above only is preferred implementation of the present invention but not limiting the scope of the invention; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the principle of the invention; can make amendment or be equal to replacement technical solution of the present invention, these modifications or be equal to replacement and also should be considered as protection scope of the present invention.
Claims (8)
1. simple and easy cultural method of EM bacterium, its feature may further comprise the steps:
In A, preparation nutrient solution, the plastic square bucket of joining ventilating cover of packing into;
B, inoculation genus bacillus;
C, airtight cultivation 24 hours;
D, inoculation compound lactobacillus, yeast, photosynthetic bacteria;
E, airtight cultivation.
2. the simple and easy cultural method of EM bacterium as claimed in claim 1 is characterized in that the nutrient solution in the steps A contains the composition of following weight part: 6% molasses water, 0.5% peptone.
3. the simple and easy cultural method of EM bacterium as claimed in claim 1 is characterized in that the long-pending 20-25L of being of the plastic square staving of joining ventilating cover in the steps A.
4. the simple and easy cultural method of EM bacterium as claimed in claim 1, the inoculum size that it is characterized in that genus bacillus among the step B is 5% of a nutrient solution weight, and the inoculum size of compound lactobacillus, yeast, photosynthetic bacteria is respectively 6%, 5%, 4% of nutrient solution weight among the step D.
5. as claim 2 or the simple and easy cultural method of 3 or 4 described EM bacterium, the culture temperature that it is characterized in that step C and step e is 20-35 ℃.
6. the simple and easy cultural method of EM bacterium as claimed in claim 5, the culture temperature that it is characterized in that step C and step e is 25-35 ℃.
7. the simple and easy cultural method of EM bacterium as claimed in claim 6 is characterized in that the time that step e is cultivated is 3-7 days.
8. the simple and easy cultural method of EM bacterium as claimed in claim 7 is characterized in that the time that step e is cultivated is 5 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010618454 CN102154106B (en) | 2010-12-31 | 2010-12-31 | Simple culture method for effective microorganisms (EM) |
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| CN 201010618454 CN102154106B (en) | 2010-12-31 | 2010-12-31 | Simple culture method for effective microorganisms (EM) |
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| CN102154106A true CN102154106A (en) | 2011-08-17 |
| CN102154106B CN102154106B (en) | 2012-12-05 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255059A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of high purity EM protospecies |
| CN103255074A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Simple and rapid propagation method of EM |
| CN105861394A (en) * | 2016-06-15 | 2016-08-17 | 佛山市百特利农业生态科技有限公司 | Aerobic mixed bacterium flora for aquaculture and preparing method thereof |
| CN106259058A (en) * | 2015-05-22 | 2017-01-04 | 郭振瑞 | A kind of fish and shrimp common disease Therapeutic Method with good result |
| CN106636214A (en) * | 2016-09-21 | 2017-05-10 | 郑华 | Ginkgo biloba exocarp fermentation liquid as well as preparation method and application thereof |
| CN112293159A (en) * | 2020-10-29 | 2021-02-02 | 青海泓辉生物科技有限公司 | Coprinus comatus culture medium subjected to anaerobic fermentation treatment and culture method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999715A (en) * | 2006-01-13 | 2007-07-18 | 苏祐隆 | Cultivating process of photosynthetic bacteria |
| CN101082028A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water-adjusting bacterium and method for restoring aquaculture environment |
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2010
- 2010-12-31 CN CN 201010618454 patent/CN102154106B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100999715A (en) * | 2006-01-13 | 2007-07-18 | 苏祐隆 | Cultivating process of photosynthetic bacteria |
| CN101082028A (en) * | 2006-06-01 | 2007-12-05 | 上海泓宝绿色水产科技发展有限公司 | Preparation of water-adjusting bacterium and method for restoring aquaculture environment |
Non-Patent Citations (2)
| Title |
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| 《 家 畜 生 态》 20011231 詹益全等 新型EM菌剂的研制 9-13 1-8 第22卷, 第3期 * |
| 《北京农业》 20031231 孙金兵 EM菌家庭培育技术 32,33 1-8 , 第3期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255059A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of high purity EM protospecies |
| CN103255074A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Simple and rapid propagation method of EM |
| CN106259058A (en) * | 2015-05-22 | 2017-01-04 | 郭振瑞 | A kind of fish and shrimp common disease Therapeutic Method with good result |
| CN105861394A (en) * | 2016-06-15 | 2016-08-17 | 佛山市百特利农业生态科技有限公司 | Aerobic mixed bacterium flora for aquaculture and preparing method thereof |
| CN106636214A (en) * | 2016-09-21 | 2017-05-10 | 郑华 | Ginkgo biloba exocarp fermentation liquid as well as preparation method and application thereof |
| CN112293159A (en) * | 2020-10-29 | 2021-02-02 | 青海泓辉生物科技有限公司 | Coprinus comatus culture medium subjected to anaerobic fermentation treatment and culture method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102154106B (en) | 2012-12-05 |
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