CN112293159A - Coprinus comatus culture medium subjected to anaerobic fermentation treatment and culture method - Google Patents
Coprinus comatus culture medium subjected to anaerobic fermentation treatment and culture method Download PDFInfo
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- CN112293159A CN112293159A CN202011179173.6A CN202011179173A CN112293159A CN 112293159 A CN112293159 A CN 112293159A CN 202011179173 A CN202011179173 A CN 202011179173A CN 112293159 A CN112293159 A CN 112293159A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses a coprinus comatus cultivation medium and a cultivation method for anaerobic fermentation treatment, wherein the coprinus comatus cultivation medium comprises the following components in parts by weight: 20-40 parts of mixed bacteria polluted mushroom bran, 10-30 parts of sawdust, 10-20 parts of bran, 10-20 parts of mushroom residue biochar, 0.2-1 part of baking soda, 1-2 parts of light calcium carbonate, 2-5 parts of EM (effective microorganism) strain and 50-70 parts of water. During cultivation, lime is added into the fermented compost to adjust the pH value, ditches are dug on the ground, strains are scattered at the bottoms of the ditches, then the compost is fully paved, the surface layer is tightly covered by the strains, and the compost is covered by a mulching film; when the hypha fully grows on the material surface, a layer of earthing material is laid; and (4) earthing, accelerating budding, and harvesting about 15 days after budding. The invention has the advantages of easy control of fermentation quality, elimination of mixed bacteria pollution, long-term storage, high utilization rate of raw materials, low planting cost, simple cultivation operation and easy popularization and utilization.
Description
Technical Field
The invention belongs to the technical field of fungus cultivation, and particularly relates to a coprinus comatus cultivation medium subjected to anaerobic fermentation treatment and a cultivation method.
Background
Coprinus comatus is also called Coprinus comatus, is commonly known as coprinus comatus, is a rare fungus with commercial potential developed manually in recent years because of the shape of the coprinus comatus is similar to that of chicken shreds in meat quality and taste, and is known as 'new in fungus'. The coprinus comatus is rich in nutrition, delicious in taste and excellent in mouthfeel, is beneficial to appetite and digestion promotion and human immunity enhancement after being frequently used, and has high nutritional value. Since the coprinus comatus is short in growth cycle, high in biotransformation rate and easy to cultivate, the coprinus comatus is particularly suitable for being planted in rural areas of China, and the planting scale is rapidly expanded in recent years, so that the coprinus comatus becomes one of the edible fungi cultured by the public in the umbrella fungi.
The mushroom bran is obtained by cultivating edible mushroom with straw, sawdust and other raw materials, and the collected culture medium remains are commonly called edible mushroom cultivation waste, mushroom residue or excess material. It is a compound of edible fungus mycelium residues and components such as crude fiber and the like, the structure of which is changed by enzymolysis of edible fungus, on one hand, the compound is changed substantially, and on the other hand, the compound is easily polluted by mixed fungi due to the fact that the compound contains a large amount of organic matters, so that the compound is difficult to continue use.
At present, coprinus comatus mainly takes cottonseed hulls, straws and the like as main culture raw materials, and is planted after being stacked, fermented and decomposed at high temperature in an aerobic way.
Disclosure of Invention
The invention aims to: aiming at the defects in the prior art, the coprinus comatus culture medium and the coprinus comatus culture method adopting anaerobic fermentation treatment are provided.
The technical scheme adopted by the invention is as follows:
a coprinus comatus culture medium subjected to anaerobic fermentation treatment comprises the following components in parts by weight:
20-40 parts of mixed bacteria polluted mushroom bran, 10-30 parts of sawdust, 10-20 parts of bran, 10-20 parts of mushroom residue biochar, 0.2-1 part of baking soda, 1-2 parts of light calcium carbonate, 2-5 parts of EM bacteria and 50-70 parts of water.
Further, the paint comprises the following components in parts by weight:
30 parts of mixed bacteria polluted fungus bran, 30 parts of sawdust, 20 parts of bran, 15 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 3 parts of EM (effective microorganisms) and 60 parts of water.
Furthermore, the EM strain is added with brown sugar with equal mass and water with 20 times of mass to dilute the EM strain before use and then cultured for 12-24h at 20-30 ℃.
The preparation method of the coprinus comatus culture medium subjected to anaerobic fermentation treatment specifically comprises the following steps: mixing the above materials, sealing, and fermenting at 20-50 deg.C for more than 15 days; during the fermentation process, turning and water supplement are not carried out.
Further, the ingredients can be stored for more than two years after mixing and sealing. The anaerobic fermentation process lasts for 15 days to 6 months, the process is sealed and is not subjected to pile turning and water supplementing, and the process can be stored for more than two years after the natural fermentation is finished and the sealed state is continuously kept.
The method for cultivating the coprinus comatus by adopting the culture medium for the anaerobic fermentation treatment comprises the following steps:
s1, adjusting the pH value of a culture medium to 6-11 by using lime;
s2, ditching on the ground, sowing coprinus comatus strains at the bottom of the ditch, then paving the ditch with the culture medium obtained in S1, covering a layer of coprinus comatus strains on the surface layer, and then covering with a mulching film;
s3, after the mycelium is processed by S2 and when the mycelium is full of the material surface in 7-15 days, paving 2-3cm of covering soil material;
s4, after the soil covering treatment of S3, moisturizing and illuminating for bud promotion within 20-25 days, and harvesting 12-18 days after bud emergence.
Further, the dimensions of the groove in S2 are: the width is 20-100cm, and the depth is 10-20 cm.
Further, the temperature in S2 is kept at 18-25 ℃, and the mulching film is lifted and ventilated for 1-3 times every day.
Further, the casing material in S3 is prepared by: adding water into under-forest humus soil, mixing until the water content is 50-60%, and adjusting pH to 7-11 with lime.
Further, the temperature is maintained at 15-25 ℃ in S4, and the relative humidity of air is 80-95%.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
according to the method for planting coprinus comatus by using the anaerobic fermentation treatment compost, mushroom bran, sawdust, bran, mushroom residue biochar and the like polluted by mixed mushrooms are used as main compost raw materials, the sources are wide, the raw material cost is low, the waste materials are recycled, the production cost can be effectively reduced, and the benefit maximization is realized.
The invention adopts the fungus chaff polluted by mixed bacteria as the main raw material, eliminates the mixed bacteria pollution on one hand and degrades the organic matters in the fungus chaff to make the fungus chaff become the nutrient components for the growth of the coprinus comatus on the other hand through the anaerobic fermentation process; and because the growth in-process of coprinus comatus has higher requirement for ventilation, the air permeability of the culture material is increased by adding the mushroom residue biochar, the air permeability is enhanced, the yield can be effectively improved, in addition, the waste raw materials after culture are further utilized, and the requirement of green production is met.
The invention adopts the fungus chaff polluted by the mixed fungi as the main raw material of the culture medium of the coprinus comatus, fully utilizes the fungus chaff as much as possible by blending, and is supplemented with a proper amount of auxiliary materials to adjust the nutrition proportion of the culture medium. The sawdust, the bran, the baking soda, the light calcium and the like are preferably selected to form the auxiliary materials of the culture medium, the mushroom bran contains more nutrient substances required by the growth of the coprinus comatus after being fermented, and the proper amount of the auxiliary materials are added to sufficiently add sufficient trace elements, amino acid nutrients and inorganic elements into the culture medium, so that the defect of the mushroom bran in terms of nutrient components is overcome, the mushroom bran, the mushroom residue biochar and the like are blended together, and then the mixture is fermented to form the culture medium with a proper proportion of nutrient elements.
According to the invention, the culture substrate is treated by anaerobic fermentation, so that the adverse effect of the mixed bacteria in the original mixed bacteria polluted fungus chaff can be effectively eliminated, on one hand, the fermentation process of the anaerobic treatment is enhanced by additionally adding EM (effective microorganisms), the growth of various originally existing but less polluted mixed bacteria in the raw materials is inhibited by the growth of dominant microorganisms, and metabolites generated by the growth of the dominant microorganisms can also play a role in degrading the mixed bacteria; the process does not need to add harmful substances, effectively ensures the nutrient components in the compost, and can produce high-quality pollution-free products by using the compost treated by the invention to plant coprinus comatus. In addition, the anaerobic fermentation mode utilizes the natural temperature rise in the microbial decomposition process, and further eliminates the influence of mixed bacteria in the raw materials in the high-temperature process of maintaining for a long time; the process does not need additional heating, and can reduce energy waste. According to the anaerobic fermentation mode, the compost is sealed and placed in the fermentation process, no waste is discharged, water replenishing and pile turning are not needed, the operation is simple and convenient, and environment-friendly green production is realized.
The coprinus comatus cultivation medium which eliminates the adverse effect of mixed fungi is used for cultivation, the yield of the coprinus comatus is obviously increased, and the coprinus comatus is large in size, thick in meat, high in quality, fast in fruiting and long in fruiting time, and has obvious advantages.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The preferred embodiment of the invention provides a method for cultivating coprinus comatus cultivation medium by adopting anaerobic fermentation treatment, which comprises the following specific steps:
(1) preparing a culture material: weighing 30 parts of fungus chaff polluted by mixed fungi, 30 parts of sawdust, 20 parts of bran, 15 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 3 parts of EM (effective microorganisms) bacteria and 60 parts of water by weight, and then uniformly mixing; wherein the EM strain is added with brown sugar with the same mass and water with the mass of 20 times to dilute the EM strain before use and then cultured for 12 hours at 25 ℃.
(2) Anaerobic fermentation: and (3) filling the mixed culture materials into a composite plastic bag, sealing, fermenting at about 30 ℃ for 6 months.
(3) Planting and sowing: adjusting pH to 7 with lime, digging trench with width of 20cm and depth of 10cm, spreading small amount of strain at bottom, spreading culture material in the trench, covering surface with strain, covering with plastic film, pressing with soil, culturing mycelium at 20 deg.C, and opening the film for 1 time each day.
(4) Covering soil and fruiting: covering the material surface with mycelia after 12 days, lifting the mulching film, spreading a layer of under-forest humus soil covering material with the pH of 7 of 2-3cm, covering the mulching film, and adjusting the temperature to about 20 ℃; the under-forest humus soil covering material is prepared by adding water into under-forest humus soil, mixing until the water content is 55%, and adjusting the pH value to 7 by using lime.
(5) And (3) fruiting management: and controlling the temperature to be about 20 ℃ and the relative air humidity to be about 90% within 23 days after covering soil, and harvesting until budding is promoted to be about 15 days after budding.
Example 2
The preferred embodiment of the invention provides a method for cultivating coprinus comatus cultivation medium by adopting anaerobic fermentation treatment, which comprises the following specific steps:
(1) preparing a culture material: weighing 40 parts of fungus chaff polluted by mixed fungi, 30 parts of sawdust, 15 parts of bran, 10 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 3 parts of EM (effective microorganisms) bacteria and 65 parts of water by weight, and then uniformly mixing; wherein the EM strain is added with brown sugar with the same mass and water with the mass of 20 times to dilute the EM strain before use and then cultured for 16h at 25 ℃.
(2) Anaerobic fermentation: and (3) putting the mixed culture materials into a composite plastic bag, sealing, fermenting at about 40 ℃ for 3 months.
(3) Planting and sowing: adjusting pH to 7 with lime, digging trench with width of 20cm and depth of 10cm, spreading small amount of strain at bottom, spreading culture material in the trench, covering surface with strain, covering with plastic film, pressing with soil, maintaining the temperature of the culture material at about 22 deg.C, and ventilating for 1 time each day.
(4) Covering soil and fruiting: covering the material surface with mycelia after 12 days, lifting the mulching film, spreading a layer of under-forest humus soil covering material with the pH of 7 of 2-3cm, covering the mulching film, and adjusting the temperature to about 18 ℃; the under-forest humus soil covering material is prepared by adding water into under-forest humus soil, mixing until the water content is 55%, and adjusting the pH value to 7 by using lime.
(5) And (3) fruiting management: and controlling the temperature to be about 18 ℃ and the relative air humidity to be about 90% within 22 days after covering soil, and harvesting until budding is promoted to be about 15 days after budding.
Example 3
The preferred embodiment of the invention provides a method for cultivating coprinus comatus cultivation medium by adopting anaerobic fermentation treatment, which comprises the following specific steps:
(1) preparing a culture material: weighing 25 parts of fungus chaff polluted by mixed fungi, 30 parts of sawdust, 10 parts of bran, 20 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 2 parts of EM (effective microorganisms) bacteria and 55 parts of water by weight, and then uniformly mixing; wherein the EM strain is added with brown sugar with the same mass and water with the mass of 20 times to dilute the EM strain before use and then cultured for 15h at 25 ℃.
(2) Anaerobic fermentation: and (3) filling the mixed culture materials into a composite plastic bag, sealing, fermenting at about 25 ℃ for 2 months.
(3) Planting and sowing: adjusting pH to 7 with lime, digging trench with width of 20cm and depth of 10cm, spreading small amount of strain at bottom, spreading culture material in the trench, covering surface with strain, covering with plastic film, pressing with soil, culturing mycelium at 20 deg.C, and opening the film for 1 time each day.
(4) Covering soil and fruiting: covering the material surface with mycelia after 12 days, lifting the mulching film, spreading a layer of under-forest humus soil covering material with the pH of 7 of 2-3cm, covering the mulching film, and adjusting the temperature to about 22 ℃; the under-forest humus soil covering material is prepared by adding water into under-forest humus soil, mixing until the water content is 55%, and adjusting the pH value to 7 by using lime.
(5) And (3) fruiting management: and (4) within 25 days after covering soil, controlling the temperature to be about 22 ℃ and the relative humidity of air to be about 80%, and harvesting until budding is promoted to be about 15 days after budding.
Example 4
The preferred embodiment of the invention provides a method for cultivating coprinus comatus cultivation medium by adopting anaerobic fermentation treatment, which comprises the following specific steps:
(1) preparing a culture material: weighing 25 parts of fungus chaff polluted by mixed fungi, 25 parts of sawdust, 20 parts of bran, 10 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 4 parts of EM (effective microorganisms) bacteria and 70 parts of water by weight, and then uniformly mixing; wherein the EM strain is added with brown sugar with the same mass and water with the mass of 20 times of the EM strain to be diluted before use and then cultured for 20h at 25 ℃.
(2) Anaerobic fermentation: and (3) filling the mixed culture materials into a composite plastic bag, sealing, fermenting at about 35 ℃ for 1 month.
(3) Planting and sowing: adjusting pH to 7 with lime, digging trench with width of 20cm and depth of 10cm, spreading small amount of strain at bottom, spreading culture material in the trench, covering surface with strain, covering with plastic film, pressing with soil, culturing mycelium at 24 deg.C, and opening the film for 1 time each day.
(4) Covering soil and fruiting: covering the material surface with mycelia after 12 days, lifting the mulching film, spreading a layer of under-forest humus soil covering material with the pH of 7 of 2-3cm, covering the mulching film, and adjusting the temperature to about 22 ℃; the under-forest humus soil covering material is prepared by adding water into under-forest humus soil, mixing until the water content is 55%, and adjusting the pH value to 7 by using lime.
(5) And (3) fruiting management: and controlling the temperature to be about 25 ℃ and the relative air humidity to be about 80% within 22 days after covering soil, and harvesting until budding is promoted to be about 15 days after budding.
Example 5
The preferred embodiment of the invention provides a method for cultivating coprinus comatus cultivation medium by adopting anaerobic fermentation treatment, which comprises the following specific steps:
(1) preparing a culture material: weighing 20 parts of fungus chaff polluted by mixed fungi, 10 parts of sawdust, 10 parts of bran, 10 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 2 parts of EM (effective microorganisms) bacteria and 50 parts of water by weight, and then uniformly mixing; wherein the EM strain is added with brown sugar with the same mass and water with the mass of 20 times to dilute the EM strain before use and then cultured for 18h at 25 ℃.
(2) Anaerobic fermentation: and (3) filling the mixed culture materials into a composite plastic bag, sealing, fermenting at about 45 ℃ for 15 days.
(3) Planting and sowing: adjusting pH to 7 with lime, digging trench with width of 20cm and depth of 10cm, spreading small amount of strain at bottom, spreading culture material in the trench, covering surface with strain, covering with plastic film, pressing with soil, culturing mycelium at 20 deg.C, and opening the film for 1 time each day.
(4) Covering soil and fruiting: covering the material surface with mycelia after 12 days, lifting the mulching film, spreading a layer of under-forest humus soil covering material with the pH of 7 of 2-3cm, covering the mulching film, and adjusting the temperature to about 20 ℃; the under-forest humus soil covering material is prepared by adding water into under-forest humus soil, mixing until the water content is 55%, and adjusting the pH value to 7 by using lime.
(5) And (3) fruiting management: and controlling the temperature to be about 20 ℃ and the relative air humidity to be about 90% within 23 days after covering soil, and harvesting until budding is promoted to be about 15 days after budding.
Comparative example 1
The culture medium comprises the following components: 30 parts of corncobs, 30 parts of sawdust, 20 parts of bran, 0.5 part of baking soda, 15 parts of mushroom residue biochar, 1 part of light calcium, 3 parts of EM (effective microorganisms) and 60 parts of water. The rest is the same as example 1.
Comparative example 2
The culture medium comprises the following components: 30 parts of mixed bacteria polluted fungus bran, 30 parts of sawdust, 20 parts of bran, 0.5 part of baking soda, 1 part of light calcium, 3 parts of EM (effective microorganisms) and 60 parts of water. The rest is the same as example 1.
Comparative example 3
The culture medium comprises the following components: 30 parts of mixed fungus polluted fungus bran, 30 parts of sawdust, 20 parts of bran, 15 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium and 60 parts of water. The rest is the same as example 1.
Comparative example 4
The culture medium was used as it was after sterilization without anaerobic fermentation treatment, and the other examples were the same as example 1.
Comparative example 5
The culture medium comprises the following components: 30 parts of mushroom bran without mixed bacteria pollution, 30 parts of sawdust, 20 parts of bran, 0.5 part of baking soda, 15 parts of mushroom residue biochar, 1 part of light calcium, 3 parts of EM (effective microorganisms) and 60 parts of water. The rest is the same as example 1.
The yield and performance of the coprinus comatus cultivated in example 1 and those cultivated in comparative examples 1-4 were compared, and the results are shown in table 1 below:
TABLE 1 Coprinus comatus yield and Performance Table
As can be seen from the above table, the coprinus comatus culture medium disclosed by the invention can obtain higher yield, and has the advantages of large size, thick meat, high quality, quick fruiting, long mushroom production time and obvious advantages; compared with a comparative example in which the fungus chaff without mixed fungus pollution is one of the main components, the invention obtains similar effects, and the method has the advantages of simple operation, energy conservation, elimination of adverse effects of mixed fungus and cultivation of coprinus comatus with good quality and high yield.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (9)
1. The coprinus comatus culture medium subjected to anaerobic fermentation treatment is characterized by comprising the following components in parts by weight:
20-40 parts of mixed bacteria polluted mushroom bran, 10-30 parts of sawdust, 10-20 parts of bran, 10-20 parts of mushroom residue biochar, 0.2-1 part of baking soda, 1-2 parts of light calcium carbonate, 2-5 parts of EM bacteria and 50-70 parts of water.
2. The coprinus comatus cultivation substrate subjected to anaerobic fermentation treatment according to claim 1, which is characterized by comprising the following components in parts by weight:
30 parts of mixed bacteria polluted fungus bran, 30 parts of sawdust, 20 parts of bran, 15 parts of fungus dreg biochar, 0.5 part of baking soda, 1 part of light calcium, 3 parts of EM (effective microorganisms) and 60 parts of water.
3. The coprinus comatus culture medium subjected to anaerobic fermentation treatment according to claim 1 or 2, wherein the EM is added with brown sugar with the same mass and water with the mass being 20 times of that of EM to dilute the EM before use, and then cultured for 12-24h at 20-30 ℃.
4. The preparation method of the coprinus comatus culture medium subjected to anaerobic fermentation treatment according to claim 1 or 2, which is characterized by comprising the following steps: mixing the above materials, sealing, and fermenting at 20-50 deg.C for more than 15 days; during the fermentation process, turning and water supplement are not carried out.
5. The method for cultivating coprinus comatus by using the anaerobic fermentation treated coprinus comatus cultivation substrate as claimed in claim 1 or 2, which is characterized by comprising the following steps:
s1, adjusting the pH value of a culture medium to 6-11 by using lime;
s2, ditching on the ground, sowing coprinus comatus strains at the bottom of the ditch, then paving the ditch with the culture medium obtained in S1, covering a layer of coprinus comatus strains on the surface layer, and then covering with a mulching film;
s3, after the mycelium is processed by S2 and when the mycelium is full of the material surface in 7-15 days, paving 2-3cm of covering soil material;
s4, after the soil covering treatment of S3, moisturizing and illuminating for bud promotion within 20-25 days, and harvesting 12-18 days after bud emergence.
6. The method of claim 5, wherein the dimensions of the trench in S2 are: the width is 20-100cm, and the depth is 10-20 cm.
7. The method of claim 5, wherein the temperature maintained in S2 is 18-25 ℃, and the mulch is lifted and ventilated 1-3 times a day.
8. The method as claimed in claim 5, wherein the casing material in S3 is prepared by: adding water into under-forest humus soil, mixing until the water content is 50-60%, and adjusting pH to 7-11 with lime.
9. The method as claimed in claim 5, wherein the temperature maintained in S4 is 15-25 deg.C and the relative humidity of air is 80-95%.
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| CN113597974A (en) * | 2021-08-26 | 2021-11-05 | 重庆市农业科学院 | Edible fungus renewable culture medium, preparation method and application |
| CN114788477A (en) * | 2022-04-01 | 2022-07-26 | 青海泓辉生物科技有限公司 | Stropharia rugosoannulata culture medium and preparation method and culture method thereof |
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| KR20160017880A (en) * | 2014-08-07 | 2016-02-17 | 김수문 | Cultivating method of mushroom using sawdust media |
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| CN105198515A (en) * | 2015-09-24 | 2015-12-30 | 临汾市沣昱盛农业开发有限公司 | Three-time fermentation method of medium raw material |
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| CN111066619A (en) * | 2019-12-31 | 2020-04-28 | 江苏培蕾基质科技发展有限公司 | Cultivation soil prepared from vinegar residue and method thereof |
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| CN114788477A (en) * | 2022-04-01 | 2022-07-26 | 青海泓辉生物科技有限公司 | Stropharia rugosoannulata culture medium and preparation method and culture method thereof |
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Inventor after: Qian Zhihui Inventor after: Li Zhaofei Inventor after: Liu Binxiao Inventor after: Feng Chengbin Inventor after: Yan Shengde Inventor after: Wang Ruobin Inventor after: Zhang Mushi Inventor after: Ma Xiwen Inventor after: Xiulimei Inventor after: Li Shunshan Inventor after: Wen Zhenxiang Inventor after: Liu Bing Inventor after: Wang Xinghui Inventor after: Xu Wenjin Inventor after: Li Jianpo Inventor after: Yuan Bo Inventor after: Gao Yihui Inventor after: Gao Bin Inventor after: Ma Guoye Inventor after: Li Ming Inventor after: Shen Zhiqing Inventor after: Li Zhaoyuan Inventor before: Qian Zhihui Inventor before: Li Zhaofei Inventor before: Liu Binxiao Inventor before: Feng Chengbin Inventor before: Yan Shengde Inventor before: Wang Ruobin Inventor before: Zhang Mushi Inventor before: Ma Xiwen Inventor before: Xiulimei Inventor before: Li Shunshan Inventor before: Wen Zhenxiang Inventor before: Liu Bing Inventor before: Wang Xinghui Inventor before: Xu Wenjin Inventor before: Li Jianpo Inventor before: Yuan Bo Inventor before: Gao Yihui Inventor before: Gao Bin Inventor before: Ma Guoye Inventor before: Li Ming Inventor before: Shen Zhiqing Inventor before: Li Zhaoyuan |
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Application publication date: 20210202 |