CN101720624A - Culture method of coprinus comatus - Google Patents
Culture method of coprinus comatus Download PDFInfo
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- CN101720624A CN101720624A CN200810196761A CN200810196761A CN101720624A CN 101720624 A CN101720624 A CN 101720624A CN 200810196761 A CN200810196761 A CN 200810196761A CN 200810196761 A CN200810196761 A CN 200810196761A CN 101720624 A CN101720624 A CN 101720624A
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- fermentation
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- soil
- earthing
- making industry
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Abstract
The invention discloses a culture method of coprinus comatus. The method comprises the following steps of: the batching of a strain culture medium, the fermentation of the culture medium, inoculation, spawn runing culture, earthing culture and fruiting. Waste materials of the paper making industry after fermentation are used as a culture substrate, and the waste materials of the paper making industry after fermentation, which is blended with a proper amount of soil, are used as an earthing material during the earthing culture, wherein the volume ratio of the waste materials of the paper making industry after fermentation to the soil is 1:1. The waste materials of the paper making industry after fermentation are used as the culture medium and the earthing material, thus the water storage property and the air permeability are good, thereby improving the physical and chemical properties of the earthing material and avoiding the hardening of earthing soil, and the coprinus comatus yield is improved by 20 percent.
Description
Technical field
The present invention relates to produce about cultivation matrix in the agriculture microorganism application method of coprinus comatus.
Background technology
Coprinus comatus is that development speed was very fast and integrate one of edible fungus variety of nutrition, health care, dietotherapy in recent years, and the mushroom body is pure white, and delicious flavour contains multivitamin and amino acid that needed by human body is wanted.Along with the gradually understanding of people to its health care, the market demand also increases fast.Coprinus comatus has a biological characteristic, and that is exactly an earthing fruiting not, and cover soil material and earthing mode are very big to the yield effect of coprinus comatus for this reason.At present China uses agricultural organic wastes such as edible fungi leftovers such as Asparagus and corncob, vinegar are poor, wood sawdust as cover soil material mostly.In the reality cultivation, these cultivation matrixes and cover soil material are the key factors that guarantees the coprinus comatus growth, and the defective of employed cultivation matrix and cover soil material maximum is in the prior art: the gas permeability that edible fungi leftovers, corncob and vinegar are poor is poor, earthing is hardened, wood sawdust trophism is poor, and cultivating rate is low.
Summary of the invention
The present invention provides the cultivation method of a kind of efficient coprinus comatus for overcoming the deficiencies in the prior art.
Technical scheme of the present invention is: comprise the bacterium culture medium batching, and the fermentation process of medium, inoculation, a bacterium cultivation, soil covering culture, fruiting, the paper-making industry waste material after the use fermentation is as cultivation matrix; Use the paper-making industry waste material after fermenting to admix an amount of soil during soil covering culture as cover soil material; Described fermentation back paper-making industry waste material is 1: 1 with the volume ratio of soil.
The present invention adopts paper-making industry waste material after the fermentation as medium and cover soil material, and retaining and good permeability have improved the physicochemical character of cover soil material, have avoided hardening of earthing, and cultivating rate has improved 20%.
Embodiment
The present invention includes the bacterium culture medium batching, the fermentation process of medium, inoculation, a bacterium cultivation, soil covering culture, fruiting, concrete implementation step is as follows:
1, the spawn culture based raw material is: the female kind adopted the PDA enriched medium, and the liquid spawn method is produced; Original seed adopts wheat medium, conical flask production; Cultivated species adopts the cotton seed hulls medium, and the plastic sack method is produced
2, cultivation bag culture medium prescription: the waste material in paper mill.
3, the fermentation process of medium is: the waste material in paper mill is smashed, and be 1 by material-water ratio again: 1-1.3 adds water and mixes evenly, piles heap, cover sunshade net fermentation reactor system, about 30 days winters, spring and autumn is about 15 days, about 10 days summers, turns over every day in summer 1 time, turn over 1 2-3 days winter.
4, fermentation material and fermentation grog: the material that ferments will in time pack.But directly use the limit pack edge joint kind of fermentation material, 4 layers of bacterial classification of 3 layered materials, two can the sleeving plastic circle, seal with old newspaper, the fermentation grog is with the material that the ferments grog bag of packing into, tightens sack with plastic ties, the autoclave of packing into after cleaning out sterilization, autoclaving 3 hours, normal-pressure sterilization 12-15 hour.
5, inoculation and cultivation: when the bag temperature is cooled to can inoculate below 30 ℃, general every bottle of cultivated species can connect cultivation bag 25-30 bag, inoculation can be adopted open, directly inoculation in booth, the cultivation bag that inoculation is good is placed on and sends out bacterium in the booth, the temperature of sending out the bacterium cultivation remains on 25-28 ℃, 30 days good mycelia of left and right sides Ke Fa.
6, the preparation of cover soil material: the waste material that the present invention is provided with the paper mill is a cover soil material.
7, take off a bag earthing: the finished product that will send out mycelia good is sloughed plastic sack, is converted into 2 sections, comes on the ground that is lined with mulch film, and the bacterium bed is transversely arranged, covers the waste material in paper mill on the bacterium bag, waits mycelia to penetrate the upper strata, when the surface begins to twist together.
8, management of producing mushroom: about 30 days, mycelia just can be covered with soil layer behind the earthing, becomes the mushroom flower bud at the charge level knot, will keep certain illumination behind the fruiting, keeps suitable ventilation to get final product.
Claims (3)
1. the cultivation method of a coprinus comatus comprises the bacterium culture medium batching, and the fermentation process of medium, inoculation, a bacterium cultivation, soil covering culture, fruiting is characterized in that: the paper-making industry waste material after the use fermentation is as cultivation matrix.
2. the cultivation method of a kind of coprinus comatus according to claim 1 is characterized in that: use the paper-making industry waste material after fermenting to admix an amount of soil as cover soil material during soil covering culture.
3. the cultivation method of a kind of coprinus comatus according to claim 2 is characterized in that: described fermentation back paper-making industry waste material is 1: 1 with the volume ratio of soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810196761A CN101720624A (en) | 2008-10-13 | 2008-10-13 | Culture method of coprinus comatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810196761A CN101720624A (en) | 2008-10-13 | 2008-10-13 | Culture method of coprinus comatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101720624A true CN101720624A (en) | 2010-06-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810196761A Pending CN101720624A (en) | 2008-10-13 | 2008-10-13 | Culture method of coprinus comatus |
Country Status (1)
| Country | Link |
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| CN (1) | CN101720624A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288501A (en) * | 2013-06-22 | 2013-09-11 | 何寒 | High nutrition covering soil for coprinus comatus soil-covering culture, and preparation method thereof |
| CN104285664A (en) * | 2013-07-19 | 2015-01-21 | 何寒 | Efficient coprinus comatus cultivation method |
| CN105112299A (en) * | 2015-08-27 | 2015-12-02 | 马鞍山市安康菌业有限公司 | Anion powder and shaggy cap high-biological-value conversion culture medium and preparation method thereof |
| CN106069181A (en) * | 2016-06-15 | 2016-11-09 | 福建省农业科学院农业生态研究所 | A kind of mushroom nutrition earthing utilizing Mushroom production roach |
| CN112385477A (en) * | 2020-11-20 | 2021-02-23 | 山东沃尔美肥业有限公司 | Secondary fruiting method of needle mushrooms |
-
2008
- 2008-10-13 CN CN200810196761A patent/CN101720624A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103288501A (en) * | 2013-06-22 | 2013-09-11 | 何寒 | High nutrition covering soil for coprinus comatus soil-covering culture, and preparation method thereof |
| CN104285664A (en) * | 2013-07-19 | 2015-01-21 | 何寒 | Efficient coprinus comatus cultivation method |
| CN105112299A (en) * | 2015-08-27 | 2015-12-02 | 马鞍山市安康菌业有限公司 | Anion powder and shaggy cap high-biological-value conversion culture medium and preparation method thereof |
| CN106069181A (en) * | 2016-06-15 | 2016-11-09 | 福建省农业科学院农业生态研究所 | A kind of mushroom nutrition earthing utilizing Mushroom production roach |
| CN112385477A (en) * | 2020-11-20 | 2021-02-23 | 山东沃尔美肥业有限公司 | Secondary fruiting method of needle mushrooms |
| CN112385477B (en) * | 2020-11-20 | 2022-06-14 | 山东沃尔美肥业有限公司 | Secondary fruiting method of needle mushrooms |
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Open date: 20100609 |