CN101530042A - Fermented processing technique of hirsutslla sinensis - Google Patents
Fermented processing technique of hirsutslla sinensis Download PDFInfo
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- CN101530042A CN101530042A CN200910136856A CN200910136856A CN101530042A CN 101530042 A CN101530042 A CN 101530042A CN 200910136856 A CN200910136856 A CN 200910136856A CN 200910136856 A CN200910136856 A CN 200910136856A CN 101530042 A CN101530042 A CN 101530042A
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Abstract
The invention relates to a processing technique field of microorganism, in particularly, a fermented processing technique of hirsutslla sinensis. The technique has culture medium as follows: egg 70-90%, milk 6-20%, potato juice 1-5%, carrot juice 1-6%, bitter salt 0.05-1%; the hirsutslla sinensis juice uses 5-10% of the culture medium weight as inoculum concentration for inoculating into a fermented container, gas-to-liquid ratio in the container is 1-3:1, the culture medium has 6-8 PH value, the working shop has 15-30 DEG. C temperature, the culture medium is dried 10-30 days for obtaining the hirsutslla sinensis. The technique has advantages of simple technique, short fermented period, no chemical residual, little pollution probability, little equipment investment, low processing cost, 1:10 input-output ratio and 10% yield rate.
Description
. technical field:
The present invention relates to the production technical field of a kind of microorganism, particularly a kind of production technology of hirsutella sinensis fungal powder fermentation.
Two. background technology:
Cordyceps sinensis is that Chinese caterpillar fungus bat moth (Hepialusarmoricamus Obcrthur) the larva infection of Chinese that belongs to of Lepidoptera bat moth is by hair spore (Hirsutella sinensis Liu, Guo, YuetZeng) behind the fungi, the entomogenous fungi complex that a large amount of in vivo breedings back forms.The fermentation method for producing of the disclosed Chinese Cordyceps sinensis fungi of Chinese patent CN97110448.4, disclosed aweto in large scale fermenting and producing of Chinese patent CN200410101728.X and technique for processing power of fungus, Chinese patent CN 03131904.1 disclosed a kind of Hirsutella sinensis liquid fungus seed process.Above-mentioned three patents all utilize hirsutella sinensis fungal to adopt liquid nutrient medium, utilize the large scale industry fermentation tank level Four submerged fermentation method production bacterium powder of expanding propagation step by step, its shortcoming is to have continued to use the antibiotic fermentation pattern, the chemical substance of medium is easily residual in the bacterium powder, the link complexity, and pollution probability is big, equipment investment is big, have a big risk, input-output ratio is little, and yield is low.
Three. summary of the invention:
The objective of the invention is at the prior art above shortcomings, a kind of production technology that adopts the static cultivation hirsutella sinensis fungal powder fermentation of liquid of development and design, it can effectively overcome deficiency of the prior art, it is simple that it has technology, pollution probability is little, equipment investment is little, and risk is little, and yield can reach series of advantages such as 10%.
Bacterial classification required for the present invention is: Hirsutella sinensis (Hirsutella sinensis Liu, Guo, Yu etZeng).Now be kept at Chinese microbial preservation administration committee common micro-organisms center (CGMCC).
Technical solution of the present invention realizes by the following method: a kind of hirsutella sinensis fungal powder, adopt activated protein medium liquid standing for fermentation technology, described activated protein medium is made by following raw materials by weight percent, medium is: eggs 70～90%, milk 6～20%, potato fruit 1～5%, carrot juice 1～6%, magnesium sulfate 0.05～1%.Described eggs are egg, goose egg, duck's egg or quail egg, and described milk can be milk, goat milk or mare's milk.
The medium of described hirsutella sinensis fungal powder is preferably: eggs 80～85%, milk 5～10%, potato fruit 2～5%, carrot juice 3～5%, magnesium sulfate 0.05～1%.
The milk weighing according to the above ratio that eggs in the above-mentioned medium, potato, carrot are squeezed respectively, get after juice, the aseptic filtration with aseptic filtration mixes, pack in the round, then Hirsutella sinensis bacterial classification liquid being pressed 5～10% of medium weight inserts in the round for inoculum concentration, gas in the round, liquid volume ratio are 1～3:1, medium pH is 6～8,15～30 ℃ of fermentation plant temperature are cultivated and were promptly got hirsutella sinensis fungal powder after the drying in 10～30 days.
Effect of the present invention is: fermentation period is short, foreshorten to 10～30 days by 45 days to 60 days, it cuts three operations, and technology is simple, adopting free of contamination agricultural byproducts is medium, so no chemical residual, pollution probability are little, equipment investment is equivalent to 1/10th of level Four submerged fermentation equipment, and equipment investment is little, production cost reduces by 50%, the fermentation process devoid of risk, input-output ratio reaches 1:10, and yield brings up to 10% by original 0.8%.
Four. embodiment:
Embodiment 1: egg, potato, carrot are squeezed, get weighing egg 70kg after juice, the aseptic filtration respectively, and potato fruit 4.95kg, carrot juice 5kg and magnesium sulfate 0.05kg mix in the round of packing into the milk 20kg of aseptic filtration.Then 5kg Hirsutella sinensis kind liquid is inserted in the round, the gas in the round, liquid volume ratio are 1:1, medium pH=6, and 30 ℃ of fermentation plant temperature are cultivated and were drying to obtain hirsutella sinensis fungal powder 20.5kg in 10 days.
Embodiment 2: duck's egg, potato, carrot are squeezed, get weighing duck's egg 80kg after juice, the aseptic filtration respectively, and potato fruit 2kg, carrot juice 6kg and 1kg magnesium sulfate mix in the round of packing into the goat milk 11kg of aseptic filtration.Then 7kg Hirsutella sinensis kind liquid is inserted in the round, the gas in the round, liquid volume ratio are 2:1, medium pH=7, and 15 ℃ of fermentation plant temperature are cultivated and were drying to obtain hirsutella sinensis fungal powder 19.8kg in 30 days.
Embodiment 3: goose egg, potato, carrot are squeezed, get weighing goose egg 85kg after juice, the aseptic filtration respectively, and potato fruit 1kg, carrot juice 3.5kg and magnesium sulfate 0.5kg mix in the round of packing into the mare's milk 10kg of aseptic filtration.Then 10kg Hirsutella sinensis kind liquid is inserted in the round, the gas in the round, liquid volume ratio are 1.5:1, medium pH=8, and 28 ℃ of fermentation plant temperature are cultivated and were drying to obtain hirsutella sinensis fungal powder 20.1kg in 18 days.
Embodiment 4: quail egg, potato, carrot are squeezed, get weighing quail egg 90kg after juice, the aseptic filtration respectively, and potato fruit 0.5kg, magnesium sulfate 0.5kg, the milk 6kg of carrot juice 3kg and aseptic filtration mixes in the round of packing into.Then 6kg Hirsutella sinensis kind liquid is inserted in the round, the gas in the round, liquid volume ratio are 3:1, medium pH=6.5, and 25 ℃ of fermentation plant temperature are cultivated and were drying to obtain hirsutella sinensis fungal powder 20.3kg in 20 days.
Claims (2)
1. a hirsutella sinensis fungal powder fermentation manufacturing technique is characterized in that, the percentage by weight of activated protein medium is: eggs 70～90%, milk 6～20%, potato fruit 1～5%, carrot juice 1～6%, magnesium sulfate 0.05～1%; Fermentation manufacturing technique is earlier with the eggs in the above-mentioned medium, potato, carrot, squeeze respectively, get after juice, the aseptic filtration with aseptic filtration after milk according to the above ratio proportioning mix, pack in the round, 5～10% inoculum concentrations of then the Hirsutella sinensis bacterial classification being pressed medium weight insert in the round, gas in the round, liquid volume ratio are 1～3:1, medium PH is 6～8,15～30 ℃ of fermentation plant temperature are cultivated and were promptly got hirsutella sinensis fungal powder after the drying in 10～30 days.
2. hirsutella sinensis fungal powder fermentation manufacturing technique according to claim 1, the percentage by weight that it is characterized in that described activated protein medium is eggs 80～85%, milk 5～10%, potato fruit 2～5%, carrot juice 3～5%, magnesium sulfate 0.05～1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910136856A CN101530042A (en) | 2009-04-16 | 2009-04-16 | Fermented processing technique of hirsutslla sinensis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910136856A CN101530042A (en) | 2009-04-16 | 2009-04-16 | Fermented processing technique of hirsutslla sinensis |
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| CN101530042A true CN101530042A (en) | 2009-09-16 |
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| CN200910136856A Pending CN101530042A (en) | 2009-04-16 | 2009-04-16 | Fermented processing technique of hirsutslla sinensis |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535185A (en) * | 2013-09-30 | 2014-01-29 | 涂峰 | Method for separating liquid of hirsutella sinensis strains |
| CN105713848A (en) * | 2016-04-29 | 2016-06-29 | 安发（福建）生物科技有限公司 | Hirsutella sinensis improved culture medium |
| CN109618811A (en) * | 2018-12-25 | 2019-04-16 | 长兴制药股份有限公司 | Industrialization cordyceps sinensis artificial culturing method |
-
2009
- 2009-04-16 CN CN200910136856A patent/CN101530042A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535185A (en) * | 2013-09-30 | 2014-01-29 | 涂峰 | Method for separating liquid of hirsutella sinensis strains |
| CN103535185B (en) * | 2013-09-30 | 2015-11-25 | 涂峰 | A kind of liquid separation method of China pilose spore bacterial classification |
| CN105713848A (en) * | 2016-04-29 | 2016-06-29 | 安发（福建）生物科技有限公司 | Hirsutella sinensis improved culture medium |
| CN109618811A (en) * | 2018-12-25 | 2019-04-16 | 长兴制药股份有限公司 | Industrialization cordyceps sinensis artificial culturing method |
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Open date: 20090916 |