CN102783565B - Preparation method of cordyceps feed additive - Google Patents
Preparation method of cordyceps feed additive Download PDFInfo
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Abstract
The invention relates to a preparation method of a feed additive, in particular to a preparation method of a cordyceps feed additive. According to the method, the cordyceps feed additive is prepared from cordyceps sinensis anamorphic strains of paecilomyces hepialid for inclined plane culture, liquid fermentation, solid fermentation and later-stage curing process, the cordyceps feed additive is different from microbial viable preparations and is different from ordinary cordyceps products, the defects of products in the prior art are overcome, the effect ingredients of the cordyceps is remained to the maximum degree, in addition, grain bran powder in production raw materials of products are converted into bifidus factors through microorganism via the degradation process in the specific high-temperature sterilization process, the animal growth can be preferably promoted, the feed conversion rate is improved, the cost is reduced, in addition, the animal immunity and the disease resistance can be improved on the premise of not using medicine or using little medicine, the survival rate is improved, and the cost is reduced.
Description
Technical field
The present invention relates to a kind of preparation method of feed addictive, specifically relate to a kind of preparation method of Chinese caterpillar fungus feed additives, adopt Anamorph of Cordyceps Sinensis bacterial strain-Paecilomyces hepiali chen to carry out the Chinese caterpillar fungus feed additives that inclined-plane cultivation, liquid fermentation, solid fermentation and later stage curing process are produced, belong to feed addictive processing technique field.
Background technology
The additive adopting in feed mostly at present is antibiotic, chemical synthetic drug, hormone etc., they are easily residual in animal body, long-term use, can make cultivated animals develop immunity to drugs or remain in the finished product (meat, egg, milk etc.) of cultivated animals, food security is constituted a threat to, directly or indirectly human health is worked the mischief, cause body impaired.Green feed additive has represented future thrust,, noresidue pollution-free from broadly referring to, disease resistance, somatotrophic natural additive, developed the dissimilar additives such as Chinese herbal and crude drugs preparations, enzyme preparation, microorganism formulation, acidulant, mould inhibitor, compound sugar at present.
Application beneficial microbe carries out the development and production of feed addictive, and a development feed product Xin road is provided.Additive for microbe feedstuff provides a kind of health, nontoxic, free of contamination selection for feed industry and its feeding.Owing to producing by microbial fermentation processes through the bacterial classification of strict research, screening gained, not only output is high, be of high nutritive value, and easy to control the quality.Strictly selecting and limiting after raw material, adding the environmental condition that strict sterilization is cultivated, its product is also pollution-free.Microorganism fodder additive is as the nuisanceless novel additive agent for feeding of a kind of high-quality, due to its biologically active, there is improving food conversion ratio, promote animal growth, improve daily gain and production performance, enhancing premunition, the unique effect such as feeding environment pollution of improving.Microorganism fodder additive brings the huge economic benefit of our being not only, ecological benefits and green livestock products, brings a new change also will to whole feedstuff industry.Some modern researchs show, Chinese caterpillar fungus artificial fermentation's mycelial main pharmacology medicine changes into and point is substantially similar to Chinese caterpillar fungus crude drug.In cordyceps mycelia, except containing Cordyceps sinensis polysaccharide, also have the materials such as amino acid, trace element, PEARLITOL 25C and ergosterol, ucleosides, vitamins, gill fungus sugar, stearic acid.In a word, the health-care effect uniqueness of Chinese caterpillar fungus and metabolite, effect is remarkable, and social required quantity grows with each passing day, and has the more optimistic prospect of marketing.In production in the past and research, be all using Chinese caterpillar fungus and metabolin thereof as medicine and health products, and existing multiple product come out.Utilize cordyceps sinensis products to make feed addictive, there is no abroad report, domestic close with this patent only have the feed addictive patent application that Chinese patent CN97107712.6 and pupa Paecilomyces varioti, Paecilomyces cicadae, paecilomyces gunniliang are material, but difference is very obvious.The former is mainly that this patent involves a wide range of knowledge, relating to known unknown bacterial classification reaches hundreds of, but Chinese caterpillar fungus not of the same race, zymotechnique difference, functional component also different, effect is not identical yet, even some product can be toxic, as stinkbug Chinese caterpillar fungus, know for sure the at present active component of Chinese caterpillar fungus and phorozoon and the kind of health care are less.The enforcement Paecilomyces varioti of this patent, this problem is that the Paecilomyces varioti of mentioning is a very large genus here, mean and comprise all Paecilomyces variotis, but the product of Paecilomyces varioti different culture media formula, different fermentations explained hereafter, effect difference, some can be used for animal health, and some can not be even harmful.Rear a few person's patents bacterial classification used is Paecilomyces varioti not of the same race, but all comparatively traditional handicraft, condition control complexity of zymotechnique.
Summary of the invention
The object of the invention is for overcoming above-mentioned the deficiencies in the prior art part, a kind of preparation method of Chinese caterpillar fungus feed additives is provided, adopt Anamorph of Cordyceps Sinensis bacterial strain-Paecilomyces hepiali chen to carry out inclined-plane cultivation, liquid fermentation, the Chinese caterpillar fungus feed additives that solid fermentation and later stage curing process are produced, it is different from microorganism live bacteria preparation, also be different from conventional cordyceps sinensis products, it has overcome the shortcoming of product in the past, retain to greatest extent the functional component of Chinese caterpillar fungus, in addition, husk powder in production raw material, after the degradation technique in specific high-temperature sterilization process, become Oilgosaccharkdes by microbial conversion, can better promote growth of animal, improve the conversion ratio of feed and reduce costs, and can be because improving animal immunizing power and disease resistance under the prerequisite of not medication or few medication, improve survival rate and reduce costs.
The present invention realizes with following technical scheme: a kind of preparation method of Chinese caterpillar fungus feed additives, is characterized in that: preparation method comprises: bacterial classification spreads cultivation, strain fermentation, later stage slaking; Described bacterial classification is peacilomyce hepiahi bacterium kind (Paecilomyces hepiali Chen et Dai), and bacterial classification spreads cultivation and comprises that original seed expands cultivation, slant tube bacterial classification is cultivated and liquid shaking bottle seed culture three steps; The fermentation of described bacterial classification comprises the fermentation of liquid seeds tank, liquid fermentation tank fermentation and solid-state fermentation tank fermentation; Described later stage slaking is by the material after solid-state fermentation tank fermentation, inserts net dish, puts into 25 DEG C of sterile workshop, carries out later stage slaking; Concrete preparation method carries out as follows:
(1) original seed expands and cultivates: by the bacterial classification access wheat berry culture medium of separation and purification, put into 22 DEG C of incubators, incubation time is 15 days, treats that mycelia covers with triangular flask and start to produce spore; Slant tube bacterial classification is cultivated, and cultured former bacterial classification is inoculated in to slant tube, puts into 22 DEG C of incubators, and cultivation 7d, treats that mycelia covers with test tube; Liquid shaking bottle seed culture, in 1000ml triangular flask, pack 400ml culture medium into, 121 DEG C of moist heat sterilization 30min, to be cooled to room temperature, in culture medium in cultured slant strains access triangular flask, be placed in constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, under 180r/m condition, cultivate, incubation time is 96h～120h;
(2) liquid fungus seed and solid fermentation: liquid seeds tank fermentation, in 100L mechanical agitating fermentation tank, pack 50L fluid nutrient medium into, pH value is 6.0～7.2, passes into wet-hot steam, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 30min.After sterilizing, be cooled to 22 DEG C, 4 bottles of cultured liquid shaking bottle seed accesses cultivated to 22 ± 1 DEG C of cultivation temperature, mixer rotating speed is 30r/m～50r/m, and logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03mpa～0.05mpa, cultivate 72h, reach the growth index phase, liquid fermentation tank is produced, in 2000L fermentation tank, pack 1000L fluid nutrient medium into, pH value is 6.0～7.2, pass into wet-hot steam, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 30min, after sterilizing, be cooled to 22 DEG C, the 50L liquid seeds of seeding tank is all accessed to liquid fermentation tank and cultivate, 22 ± 1 DEG C of incubation time cultivation temperature, mixer rotating speed is 30r/m～50r/m, logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03mpa～0.05mpa, cultivates 48h, reaches the growth index phase, solid fermentation, solid material is inserted to solid-state fermentation tank, add suitable quantity of water, its weight is material: water=9:1, after stirring, pass into wet-hot steam sterilizing, mixer rotating speed is 6r/m～15r/m, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 1h, after sterilizing, be cooled to 22 DEG C, the liquid seeds access solid fermentation culture medium of liquid fermentation tank is cultivated, inoculum concentration is every kg dry material access 100ml liquid seeds, 22 ± 1 DEG C of cultivation temperature, logical filtrated air amount is 1:0.8V/V.min, tank pressure 0.03mpa～0.06mpa, stir once every 12h, mixer rotating speed is 3r/m～5r/m, each 5min that stirs, cultivate 72h,
(3) later stage slaking: the ferment solid material of 72h of solid-state fermentation tank is put in clean net dish, is positioned in 24 DEG C of aseptic draft chambers and carries out later stage slaking, thickness of feed layer is 5cm, can dry pulverizing after slaking 72h, both crosses after 100 mesh sieves to obtain finished product.
Described slant medium formula comprises the raw material of following weight ratio: potato 100g, and wheat bran 20g, glucose 10g, yeast extract powder 5g, peptone 5g, agar powder 20g, adds water to 1L, and pH is 6.0～7.2.
Described liquid shaking bottle culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 20g, glucose 10g, yeast extract powder 5g, add water to 1L, pH is 6.0～7.2.
Described liquid seeds tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g,, dusty yeast 5g,, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, pH be 6.0～7.2.
Described liquid fermentation tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g,, dusty yeast 5g, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, and with saturated NaOH solution adjust pH be 6.8.
Described solid-state fermentation tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 250g, husk powder 50g, wheat bran 50g, corn flour 650g, MgSO
40.5g, KH
2pO
40.5g, pH are 6.0～7.2.
Advantage of the present invention is: this Chinese caterpillar fungus feed additives has unique advance: the production bacterial classification that (1), bacterial strain uses therefor are a kind of health food; Be applied to animal health and there is no product and appear on the market, have leading consciousness and top standard, its security simultaneously and validity are easy to be accepted and approve; (2), animal test results shows: not only improve food conversion ratio and cost-saving, increase sooner meat animals body weight and improve output, and can under the prerequisite of not medication or few medication, because improving its survival rate, the immunoregulation capability of enhancing animal reduce costs, only need little addition just can reach good gaining effect, the concrete use case of the comparable contrast weightening finish 10%～25%(of addition as 0.2% as shown in Table 1 and Table 2); Novel Chinese caterpillar fungus feed additives has disease-resistant, growth promoting function significantly, and have that toxic and side effect is little, drug residue free, the advantage such as have no drug resistance, be wide spectrum of new generation, efficient green feed addictive; (3), produce Chinese caterpillar fungus feed feed addictive also have raw material sources wide, easily produce, with low cost, quality controllable, easy to use, be widely used and the feature such as actual effect is good; (4), the mycelia of solid culture and the active component being secreted in residue culture matrix thereof are used as additive raw material after slaking postmenstruation together in production process, thereby maximally utilise resource, pursued larger economic and social benefit, and pollution-free; (5) validity of products advanced person, product can not only improve immunity and the production performance of animal significantly, and can improve significantly animal products quality, improve its trophic structure and local flavor.
The impact of table 1 Chinese caterpillar fungus feed additives on blue or green pin fiber crops chicken production performance
Note: through biometrics variance analysis, add Chinese caterpillar fungus feed additives and respectively organize gaining effect all significantly higher than blank group, difference reaches the level of signifiance (P<0.05).Feedstuff-meat ratio is through t check analysis: experimental group and control group be t=u=2.703 relatively, P<0.01.In table, a, b represent that difference reaches the level of signifiance (P<0.05), and A, B represent that difference reaches the utmost point level of signifiance (P<0.01)
The impact of table 2 Chinese caterpillar fungus feed additives on Production Performance of Weaning Pigs
Note: in different days, production performance index is not made comparisons, only to the blank group of identical age in days and experimental group comparative analysis.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail:
Embodiment,
The preparation method of Chinese caterpillar fungus feed additives, comprises the steps:
(1) bacterial classification is cultivated
A, original seed expand to be cultivated
By the bacterial classification access wheat berry culture medium of separation and purification, put into 22 DEG C of incubators, incubation time is 15 days, treats that mycelia covers with triangular flask and start to produce spore;
Wheat berry culture medium is: wheat berry is soaked after 24h, takes out wheat berry as for 500ml triangular flask, every bottled enter the amount of wheat berry amount to dry wheat 50g, 121 DEG C of moist heat sterilization 30min, are cooled to room temperature for subsequent use.
B, slant tube bacterial classification are cultivated
Cultured former bacterial classification is inoculated in to slant tube, puts into 22 DEG C of incubators, cultivate 7d, treat that mycelia covers with test tube;
The raw material weight ratio of slant medium is: potato 100g, wheat bran 20g, glucose 10g, yeast extract powder 5g, peptone 5g, agar powder 20g, add water to 1L, pH is wherein potato and wheat bran of 6.0～7.2(, all needs the 300ml that adds water, boil after 20min, 4 layers of filtered through gauze are got and are soaked juice).
C, liquid shaking bottle seed culture
In 1000ml triangular flask, pack 400ml culture medium into, 121 DEG C of moist heat sterilization 30min, to be cooled to room temperature, in culture medium in cultured slant strains access triangular flask, be placed in constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, under 180r/m condition, cultivate, incubation time is 96h～120h;
Liquid shaking bottle culture medium weight proportion is: bean cake powder 15g, groundnut meal 10g, white granulated sugar 20g, glucose 10g, yeast extract powder 5g, add water to 1L, pH is 6.0～7.2.
(2) liquid fungus seed and solid fermentation
A, the fermentation of liquid seeds tank
In 100L mechanical agitating fermentation tank, pack 50L fluid nutrient medium into, pH value is 6.0～7.2, passes into wet-hot steam, makes the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carries out sterilizing, and sterilization time is 30min.After sterilizing, be cooled to 22 DEG C, 4 bottles of cultured liquid shaking bottle seed accesses cultivated to 22 ± 1 DEG C of cultivation temperature, mixer rotating speed is 30r/m～50r/m, and logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03mpa～0.05mpa, cultivate 72h, reach the growth index phase;
Liquid seeds tank culture medium weight proportion is: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g,, dusty yeast 5g,, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, pH be 6.0～7.2.
B, liquid fermentation tank are produced
In 2000L fermentation tank, pack 1000L fluid nutrient medium into, pH value is 6.0～7.2, passes into wet-hot steam, makes the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carries out sterilizing, and sterilization time is 30min.After sterilizing, be cooled to 22 DEG C, the 50L liquid seeds of seeding tank is all accessed to liquid fermentation tank to be cultivated, 22 ± 1 DEG C of incubation time cultivation temperature, mixer rotating speed is 30r/m～50r/m, logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03mpa～0.05mpa, cultivates 48h, reaches the growth index phase;
Liquid fermentation tank culture medium weight proportion is: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g,, dusty yeast 5g, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, and with saturated NaOH solution adjust pH be 6.8.
C, solid fermentation
By solid material (bean cake powder 250kg, husk powder 50kg, wheat bran 50kg, corn flour 650kg, MgSO
40.5kg, KH
2pO
40.5kg, pH is 6.0～7.2) insert solid-state fermentation tank, add suitable quantity of water, its weight is material: water=9:1, after stirring, pass into wet-hot steam sterilizing, mixer rotating speed is 6r/m～15r/m, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 1h, after sterilizing, be cooled to 22 DEG C, the liquid seeds access solid fermentation culture medium of liquid fermentation tank is cultivated, inoculum concentration is 100L liquid seeds, 22 ± 1 DEG C of cultivation temperature, logical filtrated air amount is 1:0.8V/V.min, tank pressure 0.03mpa～0.06mpa, stir once every 12h, mixer rotating speed is 3r/m～5r/m, each 5min that stirs, cultivate 72h.
D, later stage slaking
The ferment solid material of 72h of solid-state fermentation tank is put in clean net dish, be positioned in 24 DEG C of aseptic draft chambers and carry out later stage slaking, thickness of feed layer is 5cm, after slaking 72h, can dry pulverizing, both cross after 100 mesh sieves to obtain finished product, after the assay was approved packaging product.
Claims (6)
1. a preparation method for Chinese caterpillar fungus feed additives, is characterized in that: preparation method comprises: bacterial classification spreads cultivation, strain fermentation, later stage slaking; Described bacterial classification is peacilomyce hepiahi bacterium kind (Paecilomyces hepiali Chen et Dai), and bacterial classification spreads cultivation and comprises that original seed expands cultivation, slant tube bacterial classification is cultivated and liquid shaking bottle seed culture three steps; The fermentation of described bacterial classification comprises the fermentation of liquid seeds tank, liquid fermentation tank fermentation and solid-state fermentation tank fermentation; Described later stage slaking is by the material after solid-state fermentation tank fermentation, inserts net dish, puts into 25 DEG C of sterile workshop, carries out later stage slaking; Concrete preparation method carries out as follows:
(1) original seed expands and cultivates: by the bacterial classification access wheat berry culture medium of separation and purification, put into 22 DEG C of incubators, incubation time is 15 days, treats that mycelia covers with triangular flask and start to produce spore; Slant tube bacterial classification is cultivated, and cultured former bacterial classification is inoculated in to slant tube, puts into 22 DEG C of incubators, and cultivation 7d, treats that mycelia covers with test tube; Liquid shaking bottle seed culture, in 1000ml triangular flask, pack 400ml culture medium into, 121 DEG C of moist heat sterilization 30min, to be cooled to room temperature, in culture medium in cultured slant strains access triangular flask, be placed in constant-temperature shaking incubator, 22 ± 1 DEG C of temperature, under 180r/min condition, cultivate, incubation time is 96h～120h;
(2) liquid fungus seed and solid fermentation: liquid seeds tank fermentation, in 100L mechanical agitating fermentation tank, pack 50L fluid nutrient medium into, pH value is 6.0～7.2, pass into wet-hot steam, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 30min, after sterilizing, be cooled to 22 DEG C, 4 bottles of cultured liquid shaking bottle seed accesses are cultivated, 22 ± 1 DEG C of cultivation temperature, mixer rotating speed is 30r/min～50r/min, logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03MPa～0.05MPa, cultivate 72h, reach the growth index phase, liquid fermentation tank is produced, in 2000L fermentation tank, pack 1000L fluid nutrient medium into, pH value is 6.0～7.2, pass into wet-hot steam, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 30min, after sterilizing, be cooled to 22 DEG C, the 50L liquid seeds of seeding tank is all accessed to liquid fermentation tank and cultivate, 22 ± 1 DEG C of cultivation temperature, mixer rotating speed is 30r/min～50r/min, logical filtrated air amount is 1:0.6V/V.min, tank pressure 0.03MPa～0.05MPa, cultivates 48h, reaches the growth index phase, solid fermentation, solid material is inserted to solid-state fermentation tank, add suitable quantity of water, its weight is material: water=9:1, after stirring, pass into wet-hot steam sterilizing, mixer rotating speed is 6r/min～15r/min, make the interior culture medium temperature of tank maintain 121 DEG C～123 DEG C and carry out sterilizing, sterilization time is 1h, after sterilizing, be cooled to 22 DEG C, the liquid seeds access solid fermentation culture medium of liquid fermentation tank is cultivated, inoculum concentration is every kg dry material access 100ml liquid seeds, 22 ± 1 DEG C of cultivation temperature, logical filtrated air amount is 1:0.8V/V.min, tank pressure 0.03MPa～0.06MPa, stir once every 12h, mixer rotating speed is 3r/min～5r/min, each 5min that stirs, cultivate 72h,
(3) later stage slaking: the ferment solid material of 72h of solid-state fermentation tank is put in clean net dish, be positioned in 24 DEG C of aseptic draft chambers and carry out later stage slaking, thickness of feed layer is 5cm, after slaking 72h, can dry pulverizing, excessively after 100 mesh sieves, gets product.
2. the preparation method of Chinese caterpillar fungus feed additives according to claim 1, it is characterized in that: described slant medium formula comprises the raw material of following weight ratio: potato 100g, wheat bran 20g, glucose 10g, yeast extract powder 5g, peptone 5g, agar powder 20g, add water to 1L, pH is 6.0～7.2.
3. the preparation method of Chinese caterpillar fungus feed additives according to claim 1, it is characterized in that: described liquid shaking bottle culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 20g, glucose 10g, yeast extract powder 5g, add water to 1L, pH is 6.0～7.2.
4. the preparation method of Chinese caterpillar fungus feed additives according to claim 1, is characterized in that: described liquid seeds tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g, dusty yeast 5g, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, pH be 6.0～7.2.
5. the preparation method of Chinese caterpillar fungus feed additives according to claim 1, is characterized in that: described liquid fermentation tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 15g, groundnut meal 10g, white granulated sugar 30g, dusty yeast 5g, MgSO
40.5g, KH
2pO
40.5g, each component mix rear moisturizing to 1L, and with saturated NaOH solution adjust pH be 6.8.
6. the preparation method of Chinese caterpillar fungus feed additives according to claim 1, is characterized in that: described solid-state fermentation tank culture medium prescription comprises the raw material of following weight ratio: bean cake powder 250g, husk powder 50g, wheat bran 50g, corn flour 650g, MgSO
40.5g, KH
2pO
40.5g, pH are 6.0～7.2.
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| CN103039739A (en) * | 2012-12-20 | 2013-04-17 | 福建师范大学 | Application of entomogenous fungi liquid fermentation mycelium as prawn feed additive |
| CN103734482B (en) * | 2014-01-20 | 2016-05-11 | 安徽康智生物科技有限责任公司 | The production method of a kind of feed addictive " mortierella Diding culture " |
| CN104381615B (en) * | 2014-12-12 | 2018-01-02 | 福建农林大学 | Produce the method and product of aweto feed addictive |
| CN109699838A (en) * | 2017-10-25 | 2019-05-03 | 江西六合生物科技有限公司 | A kind of pellet that effectively reduces is the feed addictive that sow produces weak young number |
| CN108323618A (en) * | 2017-12-29 | 2018-07-27 | 合肥迈可罗生物工程有限公司 | Application of the Cordyceps strain fermentation object as feed addictive in improving cock reproductive performance |
| CN108553487A (en) * | 2018-05-25 | 2018-09-21 | 江西国药有限责任公司 | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice |
| CN114686381A (en) * | 2022-03-28 | 2022-07-01 | 江西新龙生物科技股份有限公司 | Fungus liquid solid mixed fermentation process for in-situ sterilization and fermentation inoculation device thereof |
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| CN101002592A (en) * | 2007-01-12 | 2007-07-25 | 合肥迈可罗生物工程有限公司 | Method for producing nutrition type Chinese caterpillar fungus feed additives |
| CN101480226A (en) * | 2009-01-23 | 2009-07-15 | 熊忠辉 | Fungal fermentate feed addictive as well as preparation method and use thereof |
| CN102242070B (en) * | 2010-05-14 | 2013-05-08 | 浙江泛亚生物医药股份有限公司 | Method for artificially culturing paecilomyces cicadae and application of culturing product thereof |
| CN102286421A (en) * | 2011-09-16 | 2011-12-21 | 中国林业科学研究院森林生态环境与保护研究所 | Liquid fermentation culture method for paecilomyces lilacinus |
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