CN1095103A - Producing process of Chinese caterpillar fungus hypha fermentation - Google Patents
Producing process of Chinese caterpillar fungus hypha fermentation Download PDFInfo
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- CN1095103A CN1095103A CN 93104709 CN93104709A CN1095103A CN 1095103 A CN1095103 A CN 1095103A CN 93104709 CN93104709 CN 93104709 CN 93104709 A CN93104709 A CN 93104709A CN 1095103 A CN1095103 A CN 1095103A
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- grade fermemtation
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Abstract
The present invention is a kind of production technique that adopts the artificial culture method Cordyceps mycelium to be carried out the deep layer three grade fermemtation.The bacterial classification that it uses is bat pretty young woman Paecilomyces varioti novel species Paecilomyees hepiai Chen etDai, and the sp.nov. zymotechnique carries out slant culture → shaking table cultivation (one grade fermemtation) → seed tank culture (secondary cultivation) → big jar three grade fermemtation for separate the mycelia that obtains the Paecilomyces varioti novel species from Chinese caterpillar fungus bat pretty young woman larva body.Fermentation condition is: tank pressure 0.3～0.5kg/cm
2, air flow quantity 1: 1.5～1,200～220 rev/mins of stirring velocitys, 20～28 ℃ of temperature, culture medium prescription is: glucose 1～3%, silkworm chrysalis 0.5～2%, peptone 0.5～2%, Na
2HPO
40.05～0.15%, K
2HPO
40.1～0.2%.
Description
The present invention relates to a kind of Cordyceps mycelium that adopts artificial isolation cultivation method to obtain and carry out the production technique that submerged fermentation is cultivated, specifically refer to peacilomyce hepiahi novel species Paecilomyces Hepiai Chen et Dai, the deep layer fermenting process of sp.nov..
Cordyceps sinensis is rare traditional Chinese medicine, and is sweet in flavor and warm in property, has the nourishing the lung and the kidney function.Because it is grown in high plug area, be subjected to the restriction in weather, geographical position, yield poorly, gather difficulty, resource is in great shortage, costs an arm and a leg, and is not easy to apply.In recent years domestic the Cordyceps mycelium zymotechnique has been carried out a large amount of research, and produced Cordyceps mycelium with the fermentation engineering method and replace natural cs.Since Guangdong Microbes Inst in 1985, Livestock Research and Veterinary Academy of Qinghai Prov., Zhejiang Prov. Chinese Medicine Inst successively with regard to Cordyceps sinensis artificial culture method and zymotechnique application patent, as Chinese patent CN85102231A, CN85101971A, CN1031393A.The weak point of above patent is that pharmacologically active is extensive inadequately, and curative effect is not good enough.
The production technique that the object of the present invention is to provide a kind of Cordyceps mycelium submerged fermentation to cultivate for the development new drug provides a kind of new bulk drug, solves Cordyceps sinensis inadequate resource problem.
This processing method is:
1. fresh Chinese caterpillar fungus is cleaned up the back with antibiotic sterilization, under sterilising conditions, cuts thin slice and put on inclined-plane or the plate culture medium, under optimal temperature, cultivate, obtain mycelia after repeatedly purifying cultivate, up to obtaining pure strain.In refrigerator, preserve the pure strain that separation obtains standby.Cultivate use for liquid for enlarging bacterial classification, adopt disinfecting action that bacterial classification is inserted on the bottle inclined plane culture medium of eggplant, put in the incubator and cultivate.Slant medium formulation weight per-cent is glucose 1～3%, peptone 0.5～2%, silkworm chrysalis 0.5～2%, milk powder 0.5～2%, dipotassium hydrogen phosphate 0.1～0.2%, Sodium phosphate dibasic 0.05～0.15%, agar 2～4%, water 85～95%.
2. one grade fermemtation or first order seed prepare: bacterial classification is inserted enrich in the triangular flask in the liquid nutrient medium, put reciprocation type shaking table shaking culture, medium component is glucose, silkworm chrysalis, peptone, dipotassium hydrogen phosphate, Sodium phosphate dibasic.
3. second order fermentation or secondary seed preparation: first order seed will be cultivated in its access seeding tank after quality inspection is qualified, and substratum is the same.
4. three grade fermemtation or submerged fermentation: secondary seed will ferment in its access three grade fermemtation jar after quality inspection is qualified, inoculum size 5～10%, and substratum is the same.
5. fermentation stops, and puts jar.When three grade fermemtation arrives fermentation time, take a sample to check, put jar after meeting the fermentation termination criteria, filter and store mycelia section oven dry standby.
Characteristics of the present invention are: 1. the bacterial classification that uses is the peacilomyce hepiahi novel species.2. the pharmacologically active of leavened prod is extensive, and pharmacological evaluation proves: using the mycelia bulk drug of this explained hereafter to make oral liquid all has curative effect preferably through clinical trial certificate to chronic hepatitis B, acquired immune deficiency syndrome (AIDS) etc.3. add silkworm chrysalis in the substratum, mycelial growth is luxuriant, and it is then not long not add silkworm chrysalis.
Embodiment:
Zymotechnique of the present invention is a three grade fermemtation technology, and its flow process is:
1. slant strains preparation: get glucose 20 grams, silkworm chrysalis 10 grams, peptone 10 grams, milk powder 10 grams, agar 20 grams, 1000 milliliters in water is mixed with slant medium and inserts bacterial classification again, cultivates 15 days bevel bacterial classifications down in 26 ℃ in incubator.
2. shaking table is cultivated: by above-mentioned culture medium prescription (except that milk, agar), make liquid nutrient medium and pack in the 500ml triangular flask access bacterial classification, shaking culture on shaking table into.
3. fermentation culture: the same shaking table of substratum, inoculum size 8%, fermentation condition: 26 ℃ of temperature, tank pressure 0.4kg/cm, air flow quantity 1: 0.5～1,220 rev/mins of stirring velocitys.Fermentation time: seeding tank 50 hours, submerged fermentation jar 84 hours goes out jar when reaching termination fermentation standard on inspection.
4. the processing of fermented product: the mycelium fermentation broth that will go out jar is by Plate Filtration, with the mycelia piece section oven dry that leaches.Dried mycelia after qualified on inspection can make the natural cs surrogate.This ferment cordyceps sinensis mycelia can make the raw material of dietary supplements and medicine.
The main chemical compositions of finished product and natural morning the grass basically identical, mycelia is beige or brown drying slice, mildly bitter flavor is savory, pH value 4.5～5.5, and water content is no more than 6.5%, ash content is no more than 6%, water-soluble extractives is not less than 30%, and uridine is not less than 0.06%, and VITAMIN B4 is not less than 0.003%, uridylic is not less than 0.02%, and N.F,USP MANNITOL is not less than 5%.
Claims (5)
1, a kind of production technique that adopts the Cordyceps mycelium peacilomyce hepiahi novel species submerged fermentation of artificial culture, it is characterized in that having added in the strain fermentation substratum silkworm chrysalis 0.5～2%, be total to three grade fermemtation and finish through shaking table cultivation, seeding tank, big jar, two, the three grade fermemtation inoculum size is 5～10%, and quality control index is pH value, moisture, ash content, uridine, VITAMIN B4, uridylic, N.F,USP MANNITOL, water-soluble extractives.
2, zymotechnique according to claim 1, the culture medium prescription weight percent that it is characterized in that spawn culture is a glucose 1～3%, peptone 0.5～2%, silkworm chrysalis 0.5～2%, milk powder 0.5～2%, Na
2HPO
40.05～0.15%, K
2HPO
40.1～0.2%, agar 2～4%, water 85～95%.
3, zymotechnique according to claim 1 is characterized in that: one grade fermemtation shaking table culture temperature is 24～28 ℃, and incubation time is 60～72 hours, and shaking speed is 110～250 rev/mins.
4, zymotechnique according to claim 1 is characterized in that: a jar temperature is 24～28 ℃, and tank pressure is 0.5～1kg/cm, air flow quantity is 1: 0.5～1, stirring velocity is 200～220 rev/mins, and the second order fermentation time is 48～60 hours, and the three grade fermemtation time is 72～96 hours.
5, zymotechnique according to claim 1, it is characterized in that: the fermented hypha main chemical compositions is consistent with natural cs, pH value 4.5～5.5, water content is less than 6.5%, and ash content is less than 6%, and water-soluble extractives content is greater than 30%, uridine content is greater than 0.6%, VITAMIN B4 is greater than 0.003%, and uridylic is greater than 0.02%, and N.F,USP MANNITOL is greater than 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93104709 CN1095103A (en) | 1993-05-07 | 1993-05-07 | Producing process of Chinese caterpillar fungus hypha fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93104709 CN1095103A (en) | 1993-05-07 | 1993-05-07 | Producing process of Chinese caterpillar fungus hypha fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1095103A true CN1095103A (en) | 1994-11-16 |
Family
ID=4985305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93104709 Pending CN1095103A (en) | 1993-05-07 | 1993-05-07 | Producing process of Chinese caterpillar fungus hypha fermentation |
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| CN (1) | CN1095103A (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5939310A (en) * | 1996-12-05 | 1999-08-17 | Republic Of Korea Represented By Rural Development Administration | Cultivation of Paecilomyces sp. using silkworms |
| CN1055967C (en) * | 1996-12-13 | 2000-08-30 | 张世伟 | Cordyceps sinensis health-care nutrient liquor produced by submerged fermentation method |
| CN1091150C (en) * | 1999-10-27 | 2002-09-18 | 樊美珍 | Mortierella Diding, its isolation cultivation method and fermentation technology |
| CN1100562C (en) * | 1999-09-01 | 2003-02-05 | 朱宏图 | Method for preparation of fungus powder medicine of artificial cultivated cordyceps |
| CN1127917C (en) * | 2000-10-09 | 2003-11-19 | 北京长寿保健品有限公司 | Nutrient health-care food with function of slowdown senility |
| CN1320101C (en) * | 2005-09-19 | 2007-06-06 | 王永年 | Aweto cytosol preparing process |
| CN101991614A (en) * | 2010-09-13 | 2011-03-30 | 吴赣英 | Compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine and preparation thereof |
| CN102783565A (en) * | 2012-08-10 | 2012-11-21 | 徐州瀚韵生物科技有限公司 | Preparation method of cordyceps feed additive |
| CN102978123A (en) * | 2012-12-08 | 2013-03-20 | 包海鹰 | Immune activity study on ergosterol of paecilomyces hepiali |
| CN103045548A (en) * | 2012-12-10 | 2013-04-17 | 浙江工业大学 | Cordyceps sinensis ribonucleotide reductase as well as coding gene and application thereof |
| CN101560268B (en) * | 2009-05-31 | 2013-11-20 | 江西济民可信集团有限公司 | Cs-4 fermentation mycelium polysaccharide and preparation method and applications thereof |
| CN105795283A (en) * | 2016-03-21 | 2016-07-27 | 深圳市德福康泰生物科技有限公司 | Cordyceps sinensis beverage and preparation method thereof |
| CN108676730A (en) * | 2018-05-25 | 2018-10-19 | 江西国药有限责任公司 | A kind of fermentation manufacturing technique of Paecilomyces hepiali chen Cs-4 |
-
1993
- 1993-05-07 CN CN 93104709 patent/CN1095103A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5939310A (en) * | 1996-12-05 | 1999-08-17 | Republic Of Korea Represented By Rural Development Administration | Cultivation of Paecilomyces sp. using silkworms |
| CN1055967C (en) * | 1996-12-13 | 2000-08-30 | 张世伟 | Cordyceps sinensis health-care nutrient liquor produced by submerged fermentation method |
| CN1100562C (en) * | 1999-09-01 | 2003-02-05 | 朱宏图 | Method for preparation of fungus powder medicine of artificial cultivated cordyceps |
| CN1091150C (en) * | 1999-10-27 | 2002-09-18 | 樊美珍 | Mortierella Diding, its isolation cultivation method and fermentation technology |
| CN1127917C (en) * | 2000-10-09 | 2003-11-19 | 北京长寿保健品有限公司 | Nutrient health-care food with function of slowdown senility |
| CN1320101C (en) * | 2005-09-19 | 2007-06-06 | 王永年 | Aweto cytosol preparing process |
| CN101560268B (en) * | 2009-05-31 | 2013-11-20 | 江西济民可信集团有限公司 | Cs-4 fermentation mycelium polysaccharide and preparation method and applications thereof |
| CN101991614A (en) * | 2010-09-13 | 2011-03-30 | 吴赣英 | Compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine and preparation thereof |
| CN102783565A (en) * | 2012-08-10 | 2012-11-21 | 徐州瀚韵生物科技有限公司 | Preparation method of cordyceps feed additive |
| CN102978123A (en) * | 2012-12-08 | 2013-03-20 | 包海鹰 | Immune activity study on ergosterol of paecilomyces hepiali |
| CN103045548A (en) * | 2012-12-10 | 2013-04-17 | 浙江工业大学 | Cordyceps sinensis ribonucleotide reductase as well as coding gene and application thereof |
| CN105795283A (en) * | 2016-03-21 | 2016-07-27 | 深圳市德福康泰生物科技有限公司 | Cordyceps sinensis beverage and preparation method thereof |
| CN108676730A (en) * | 2018-05-25 | 2018-10-19 | 江西国药有限责任公司 | A kind of fermentation manufacturing technique of Paecilomyces hepiali chen Cs-4 |
| CN108676730B (en) * | 2018-05-25 | 2021-02-19 | 江西国药有限责任公司 | Fermentation production process of paecilomyces hepiali Cs-4 |
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