CN108553487A - A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice - Google Patents
A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice Download PDFInfo
- Publication number
- CN108553487A CN108553487A CN201810512842.3A CN201810512842A CN108553487A CN 108553487 A CN108553487 A CN 108553487A CN 201810512842 A CN201810512842 A CN 201810512842A CN 108553487 A CN108553487 A CN 108553487A
- Authority
- CN
- China
- Prior art keywords
- drying
- preparation
- pill
- mycelium
- materical crude
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
Abstract
The present invention relates to a kind of preparation methods of 4 medicine materical crude slice of fermentation cordyceps Cs, and this approach includes the following steps：Strain culturing obtains qualified hypha fluid；Qualified hypha fluid is collected, filtering, drying obtain mycelium；Using the mycelium of double-cone dryer drying drying；Pill is made in mycelium after drying；Oven drying pill；It crushes, mix, being packaged to be 4 medicine materical crude slice of fermentation cordyceps Cs.The method of the present invention carries out slowly multiple drying to mycelium, protects the medicinal ingredient in fermentation cordyceps well, improves the medical value of fermentation cordyceps.In addition, the color and luster of 4 medicine materical crude slice of fermentation cordyceps Cs made from this law is good, smell is fragrant, it is easy to carry, easy to use.
Description
Technical field
The present invention relates to pharmaceutical field, specially a kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice.
Background technology
Cordyceps sinensis also known as cordyceps sinensis, cordyceps, cordyceps sinensis are by the winter of the snakelike snakelike Cordyceps of cordyceps sinensis section of Hypocreales
Worm summer grass bacterium parasitizes the Hepialus larva in alpine meadow soil, so that larva body is ossify, and under optimum conditions, summer is by bombys batryticatus
Head end pumping goes out long rod-shaped stroma and is formed, i.e., the complex that the fructification Yu bombys batryticatus sclerotium of aweto are constituted.Winter worm
Summer grass, which has, adjusts the multiple efficacies such as function of immune system, antitumor, antifatigue, invigorating the lung and the kidney, hemostasis and phlegm and secret lean gas.
Because its yield is successively decreased year by year, and demand increases year by year, and researcher passes through unremitting effort, realizes artificial fermentation side finally
Formula cultivates cordyceps mycelia.Cultivating the most common method of cordyceps mycelia is：Obtain strain, then inclined-plane culture, shaking flask culture,
Mycelium is then obtained by filtration in seed tank culture and fermented and cultured, dries, pulverizes and sieves, mixes, is packaged to be cordyceps.
The existing technique for preparing cordyceps is all after drying the mycelium being obtained by filtration, directly to crush, then mix
Conjunction obtains product.Existing preparation process pertains only to primary drying, and there are various problems for single drying.If only dry with baking oven
Dry, drying time is long, and largely stacking can cause inside to be not easy drying thoroughly；If being dried with bipyramid merely, due to not
Control temperature and time well, it is easy to lead to product " burning ".
The unripe processing technology of fermentation cordyceps medicine materical crude slice at present, causes the medicinal material utilization rate of script high value low
Under.Therefore, it in order to improve the medical value of cordyceps, needs to study a kind of preparation method of new cordyceps medicine materical crude slice, be somebody's turn to do
Method wants that the original medical value of cordyceps can be protected.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of fermentation cordyceps Cs-4 medicine materical crude slice, improve the fermentation of strain
Method improves mycelial drying means, preferably protects the original medicinal ingredient of cordyceps, improves medical value.
Specifically, in view of the deficiencies of the prior art, the present invention provides following technical solutions：
A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice, this approach includes the following steps：
S1. strain culturing obtains qualified hypha fluid；
S2. the hypha fluid that collection step S1 is obtained, filtering, drying, obtains mycelium；
S3. the mycelium for using double-cone dryer drying steps S2 to obtain；
S4. pill is made in the step S3 mycelium dried；
S5. the pill that prepared by drying steps S4；
S6. the pill after pulverising step S5 dryings, obtains medicinal powder；
S7. mixed powder is packaged to be fermentation cordyceps Cs-4 medicine materical crude slice.
Bipyramid dries mycelium, and pill is then made, and continues pill drying, this process is to filtered water content height
Mycelium carry out slowly multiple drying, can mycelium be dried thoroughly and be prevented the dry activity for excessively leading to bacterium powder
Component is destroyed, and the medical value of fermentation cordyceps Cs-4 medicine materical crude slice is improved.
Preferably, the strain culturing described in step S1 includes inclined-plane culture, female bottle culture, seed tank culture, breeding tank training
Foster and fermentation tank culture；Wherein, the nutrient media components of inclined-plane culture include：Glucose, peptone, wheat bran, potassium dihydrogen sulfate, sulphur
Sour magnesium, agar and water；The nutrient media components of female bottle culture include：Glucose, peptone, wheat bran, potassium dihydrogen phosphate, magnesium sulfate and
Water.
Preferably, the nutrient media components of seed tank culture, breeding tank culture and fermentation tank culture include：Soybean cake powder,
Glucose, sucrose, soya-bean oil, potassium dihydrogen phosphate, magnesium sulfate and water.
Preferably, the filtering described in step S2 is selected from plate-frame filtering method, high speed centrifugation method, bag filter process and pillar
One kind in filtration method, preferably plate-frame filtering method；Filtration time is 3-5 hours, pressure 0.25-0.35MPa.
Preferably, described in step S3 double-cone dryer work when chuck pressure 0.15-0.25MPa, vacuum degree be-
0.05Mpa to -0.04Mpa, 50-90 DEG C of temperature, drying time are 8-18 hours.
Preferably, the mycelial solid content that step S2 is obtained is 30-40%.
Preferably, the mycelial water content after the drying of step S3 bipyramids is less than 55%.
Preferably, the solid content of the pill after step S5 dryings is 85% or more.
Preferably, the drying described in step S5 is oven drying, and pill made of step S4 is before entering oven drying
Resting period within 24 hours.
Preferably, the drying described in step S2 dries up 1-2 hours using the air that pressure is 0.1-0.2Mpa；Step
The temperature crushed described in rapid S6 is 25-28 DEG C, is crushed 2 times altogether.
Compared with prior art, it effect of the invention and has an advantage that：
(1) self-made medium gradually expands culture Chinese caterpillar fungus strain, qualified hypha fluid, bacterium is obtained in last fermentation tank
The concentration of filament is 15% or more.
(2) it uses that bipyramid is dry, pill and oven drying is made successively the mycelium being obtained by filtration is carried out and is slowly multiple
Dry, medicinal ingredient that can well in protected fermentation cordyceps improves the medicinal valence of fermentation cordyceps Cs-4 medicine materical crude slice
Value.
(3) so that mycelium is slowly molded by controlling temperature and time in entire preparation process, obtain ferment cordyceps sinensis
Bacterium powder Cs-4 medicine materical crude slice.Fermentation cordyceps Cs-4 medicine materical crude slice is good with color and luster, smell is fragrant, can the features such as easily swallowing, be convenient for carrying
It swallows or stews edible with directly soaked, meet the use needs under different scenes.
Specific implementation mode
The preparation method of fermentation cordyceps Cs-4 medicine materical crude slice of the present invention includes the following steps：
S1. strain culturing obtains qualified hypha fluid：The isolated bacterial strain from the fresh cordyceps sinensis in Qinghai
Paccilomyces hepiali Cs-4, carry out inclined-plane culture first, then select mycelia and grow plentiful, spore mostly and without miscellaneous
The bacterium colony of bacterium pollution carries out female bottle culture, when bacterial strain concentration is 10% or more, and residual sugar, residual nitrogen and pH meet the requirements, moves
Enter seed tank culture, sequentially enter breeding tank and fermentation tank culture again later, obtains qualified hypha fluid.
S2. the hypha fluid in fermentation tank, filtering, the mycelium moistened are collected；The preferred plate-frame filtering of filter method,
Filtration time is 3-5 hours, pressure 0.25-0.35MPa.After filtering, the air blow drying bacterium for being 0.1-0.2MPa with pressure
Filament 1-2 hours obtains the mycelium that solid content is 30-40%.
S3. the mycelium dried up after double-cone dryer dry filter is used：Chuck pressure when drying machine works is 0.15-
0.25MPa, vacuum degree are -0.05Mpa to -0.04Mpa, and 50-90 DEG C of temperature, drying time is 8-18 hours.Bacterium after drying
Filament water content is less than 55%.
S4. pill is made in the mycelium after bipyramid is dried；Pill obtained is uniformly fitted into stainless steel pallet, is controlled
Stainless steel pallet traditional Chinese medicine pill thickness processed is layer 2-3.
S5. within 24 hours, in preferably 12 hours, the stainless steel pallet equipped with pill is sent into oven drying.Baking oven
Interior temperature is 50-90 DEG C, and vacuum degree is -0.04Mpa, and drying time 18-26 hour, the solid content of pill is 85% after drying
More than.
S6. the pill after oven drying is crushed, medicinal powder is obtained；The temperature of crushing is 25-28 DEG C, is crushed 2 times altogether；It crushes
Obtained medicinal powder has 95% can sieve (120 mesh) by No. 7.
S7. in the environment of 25-28 DEG C, then mixed powder is packaged to be fermentation cordyceps Cs-4 medicine materical crude slice.
In a preferred embodiment, (for w as the weight of solid material, v is the total volume of culture medium), inclined-plane are counted using w/v
The group of culture medium becomes：Glucose 2-5%, peptone 0.2-0.5%, wheat bran 4-10%, potassium dihydrogen phosphate 0.1-0.2%, sulphur
Then sour magnesium 0.02-0.03%, agar 2-5% add water to amount of preparation (the i.e. total volume v) of culture medium.The group of female bottle culture medium
Become：Glucose 2-5%, peptone 0.4-1.0%, wheat bran 4-10%, potassium dihydrogen phosphate 0.1-0.2%, magnesium sulfate 0.02-
0.03%, then add water to amount of preparation.The condition of inclined-plane culture is：23 ± 3 DEG C of constant temperature, humid control are cultivated 80~95%
5-7 days.The condition of female bottle culture is：In 23 ± 3 DEG C of thermostatic chambers, humid control is set 80~95% and shakes culture on shaking table
3-4 days.
In another preferred embodiment, the culture medium in seeding tank, breeding tank and fermentation tank is by soybean cake powder, grape
Sugar, sucrose, soya-bean oil, potassium dihydrogen phosphate, magnesium sulfate and water composition, pH are but the different trainings naturally, pH Numerical Controls are in 6.8-7.8
The proportioning for supporting each component in tank is different.Temperature in culture tank is 23 ± 6 DEG C, and tank presses 0.03-0.07Mpa, incubation time 50-
90 hours.
The present invention is further described with specific embodiment below.
It should be noted that material and equipment that the present invention uses are commercially available；Wherein, strain
Paccilomyceshepiali Cs-4 are isolated from the fresh cordyceps sinensis in Qinghai, and the strain was on October 21st, 1997
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO.0327.
Embodiment 1
Strain separating
Fresh aweto nucleome is cleaned, repeatedly rinsed, sterilized with sterile water, aseptically, is cut outer
Skin avoids enteron aisle, cut it is fair and clear be organized as thin slice, be connected to and be separately cultured primary surface, at a temperature of 15 DEG C, static gas wave refrigerator 7 days, in vain
Color mycelia can grow from thin slice, and white hypha is further isolated and purified, aweto strain, i.e. Cs-4 can be obtained.
Expand culture Chinese caterpillar fungus strain Paccilomyces hepiali Cs-4, obtains qualified hypha fluid
Strain culturing process sees below step, and 3 batches of Chinese caterpillar fungus strains, the inclined-plane culture of each batch have been cultivated in the present embodiment expansion
It is shown in Table 1 with the culture medium composition and condition of culture of female bottle culture.The dosage of each raw material (is trained relative to total amount of preparation in table 1
Base total volume v) is supported to be calculated.
The culture medium composition and condition of culture on 1. inclined-plane of table and female bottle culture
(1) inclined-plane culture
A, prepared by medium slant：
The wheat bran for taking weighing, adds drinking water to boil half an hour, filters to take filtrate, and other raw materials are added, with drinking water dissolution
It is diluted to amount of preparation, is then distributed into test tube, loading amount is 15-20mL/ branch (can also be distributed into eggplant-shape bottle, 60mL/ branch),
Tampon beyond the Great Wall bandages, and is put into high pressure steam sterilization in sterilizing cabinet, steam pressure 0.09-0.11Mpa, 121 DEG C of temperature, sterilizing
30 minutes time, it is cooling after bevel as required.
B, inclined plane inoculating culture：
The strain stored is taken out, aseptically, original seed 1cm is taken with inoculation scoop shovel2Left and right size uniformly applies
It is distributed on inclined-plane, tampon bandages on bottle plug, and 23 ± 3 DEG C of constant temperature, humid control are cultivated 5-7 days 80~95%, to be grown
Maturation carries out visual examination, cultural characteristic：Spore and mycelia are plentiful, white or faint yellow lint shape.Reject appearance growth not
The qualified inclined-plane of growth is placed in 1-8 DEG C of refrigerator and saved backup by good inclined-plane.
(2) female bottle culture
A, prepared by female bottle culture medium：
It takes the wheat bran of weighing that drinking water is added to boil half an hour, filters, take filtrate.Other raw materials are proportionally added into, with drinking
Water dissolution is diluted to amount of preparation, is then distributed into triangular flask.750mL triangular flask charge weights are 200mL, are stoppered tampon, are wrapped up
It is good, it is put into high pressure steam sterilization in sterilizing cabinet, 0.09-0.11Mpa pressure, 120 ± 1 DEG C of steam sterilizings 30 minutes are cooled to
Less than 25 DEG C rears can be used.
B, female bottle inoculated and cultured：
Aseptically, 1cm is taken with inoculation scoop2Inclined-plane culture mycelia grows plentiful, spore mostly and the bacterium without miscellaneous bacteria
Fall the female culture in glassware of access.In 23 ± 3 DEG C of thermostatic chambers, humid control sets shaking culture 3-4 days on shaking table 80~95%.
C, and bottle：
Aseptically, 10-15 bottles (mother's 750mL bottle) female bottle seed is merged into inoculation steel cylinder.From inoculation steel cylinder
It is interior to draw a small amount of kind of liquid for doing steriling test, take the amount of a female bottle to carry out interassay, examine or check mycelial concentration, residual sugar, residual nitrogen,
PH；Through examining without microbiological contamination, bacterium is dense >=and 10%, interassay meets the requirements, and seeding tank is accessed using pressure differential method.
(3) seeding tank, breeding tank and fermentation tank culture
Based on culture medium total volume v, solid material is matched according to w/v, and liquid material is calculated according to v/v, each to cultivate
Culture medium composition and condition of culture in tank are shown in Table 2.Vaccination ways are pressure differential method.Condition phase of three batches of bacterial strains into tank culture
Together.
The culture medium composition and condition of culture of 2. seeding tank of table, breeding tank and fermentation tank
When the bacterium in seeding tank it is dense >=8% and without miscellaneous bacteria, then be seeded to breeding tank culture；Breed tank in bacterium it is dense >=8%
And it without miscellaneous bacteria, is then seeded in fermentation tank；Bacterium in fermentation tank is dense >=and 15% and without miscellaneous bacteria, as qualified hypha fluid.
The present embodiment is prepared three batches of qualified Chinese caterpillar fungus hypha body fluid altogether, respectively hypha fluid 1., hypha fluid 2.
3. with hypha fluid；Hypha fluid obtained can be used for preparing cordyceps Cs-4 medicine materical crude slice.
Embodiment 2
The preparation of fermentation cordyceps Cs-4 medicine materical crude slice
1. qualified hypha fluid is collected from fermentation tank, hypha fluid is pumped into flame filter press with pump and was carried out
Filter.Control filter pressure is 0.35MPa, and filtration time is 4 hours.It is 26 DEG C in environment temperature control after filtering, air
It is the mycelium being obtained by filtration with air blow drying 2 hours under 0.2MPa to dry up pressure, obtains the mycelium that solid content is 35%.
By in the mycelium input double-cone dryer of drying, opens vacuum and steam valve is dried in vacuo, chuck pressure
Power is 0.25MPa, and vacuum degree is -0.04Mpa, and 65 DEG C of temperature is 10 hours dry.After 8 hours dry, used every half an hour
The sampling of non-sticky sampling shovel carries out water content detection, when thalline moisture ＜ 55%, can be taken off.
Extrusion pellet processing machine is added in mycelium after taking the mycelium of bipyramid dry mass qualification, medication shovel to dry bipyramid
Hopper opens extrusion pellet processing machine switch, carries out pill, and obtained pill is uniformly fitted into stainless steel pallet, controls stainless steel
Pill thickness in pallet is 2 layers.Storage 4 hours after, the stainless steel pallet equipped with pill is put into baking oven, open vacuum and
Steam is dried；Vacuum degree be -0.04Mpa, 65 DEG C of temperature, 20 hours drying times, obtain solid content be 85% mycelia
Body product.
Pill after the completion of above-mentioned drying is slowly put into Roughpulverizer, waits for crushing one again after the completion of crushing for the first time
Secondary, it is 25 DEG C to crush temperature；Cordyceps after crushing can be sieved by No. 7 medicines.Then the bacterium powder completed will be crushed to put into
In clean two-dimensional mixing machine, it is 28 DEG C, is mixed 30 minutes under normal pressure in control room temperature.Mixed bacterium powder is in an aseptic environment
Packing, obtains fermentation cordyceps Cs-4 medicine materical crude slice finished products.The powder for the fermentation cordyceps Cs-4 medicine materical crude slice that this law is prepared
Carefully, color and luster is good, pleasant aroma.
Embodiment 3
The preparation of fermentation cordyceps Cs-4 medicine materical crude slice
2. qualified hypha fluid is collected from fermentation tank, hypha fluid is pumped into flame filter press with pump and was carried out
Filter.Control filter pressure is 0.30MPa, and filtration time is 5 hours.It is 26 DEG C in environment temperature control after filtering, air
It is the mycelium being obtained by filtration with air blow drying 2 hours under 0.1MPa to dry up pressure, obtains the mycelium that solid content is 30%.
By in the mycelium input double-cone dryer of drying, opens vacuum and steam valve is dried in vacuo, chuck pressure
Power is 0.15MPa, and vacuum degree is -0.05Mpa, temperature 50 C, 18 hours drying times.After 15 hours dry, Mei Geban
Hour carries out water content detection with the sampling of non-sticky sampling shovel, when thalline moisture ＜ 55% can be taken off.
Extrusion pellet processing machine is added in mycelium after taking the mycelium of bipyramid dry mass qualification, medication shovel to dry bipyramid
Hopper opens extrusion pellet processing machine switch, carries out pill, and obtained pill is uniformly fitted into stainless steel pallet, controls stainless steel
Pill thickness in pallet is 3 layers.After storage 12 hours, the stainless steel pallet equipped with pill is put into baking oven, vacuum is opened
It is dried with steam；Vacuum degree be -0.04Mpa, 90 DEG C of temperature, 18 hours drying times, obtain solid content be 88% bacterium
Filament product.
Pill after the completion of above-mentioned drying is slowly put into Roughpulverizer, waits for crushing one again after the completion of crushing for the first time
Secondary, it is 27 DEG C to crush temperature；Cordyceps after crushing have 95% can be sieved by No. 7 medicines.Then the bacterium powder completed will be crushed
Input in clean two-dimensional mixing machine, is 25 DEG C, is mixed 30 minutes under normal pressure in control room temperature.Mixed bacterium powder is sterile
It is dispensed under environment, obtains fermentation cordyceps Cs-4 medicine materical crude slice finished products.The fermentation cordyceps Cs-4 medicine materical crude slice that this law is prepared
Powder is thin, color and luster is good, pleasant aroma.
Embodiment 4
The preparation of fermentation cordyceps Cs-4 medicine materical crude slice
3. qualified hypha fluid is collected from fermentation tank, hypha fluid is pumped into flame filter press with pump and was carried out
Filter.Control filter pressure is 0.25MPa, and filtration time is 3 hours.It is 26 DEG C in environment temperature control after filtering, air
It is the mycelium being obtained by filtration with air blow drying 1 hour under 0.2MPa to dry up pressure, obtains the mycelium that solid content is 40%.
By in the mycelium input double-cone dryer of drying, opens vacuum and steam valve is dried in vacuo, chuck pressure
Power is 0.20MPa, and vacuum degree is -0.04Mpa, 90 DEG C of temperature, 8 hours drying times.It is small every half after 5 hours dry
When with non-sticky sampling shovel sampling carries out water content detection, when thalline moisture ＜ 55% be can be taken off.
Extrusion pellet processing machine is added in mycelium after taking the mycelium of bipyramid dry mass qualification, medication shovel to dry bipyramid
Hopper opens extrusion pellet processing machine switch, carries out pill, and obtained pill is uniformly fitted into stainless steel pallet, controls stainless steel
Pill thickness in pallet is 2 layers.After storage 24 hours, the stainless steel pallet equipped with pill is put into baking oven, vacuum is opened
It is dried with steam；Vacuum degree be -0.04Mpa, temperature 50 C, 26 hours drying times, obtain solid content be 90% bacterium
Filament product.
Pill after the completion of above-mentioned drying is slowly put into Roughpulverizer, waits for crushing one again after the completion of crushing for the first time
Secondary, it is 28 DEG C to crush temperature；Cordyceps after crushing have 95% can be sieved by No. 7 medicines.Then the bacterium powder completed will be crushed
Input in clean two-dimensional mixing machine, is 26 DEG C, is mixed 30 minutes under normal pressure in control room temperature.Mixed bacterium powder is sterile
It is dispensed under environment, obtains fermentation cordyceps Cs-4 medicine materical crude slice finished products.The fermentation cordyceps Cs-4 medicine materical crude slice that this law is prepared
Powder is thin, color and luster is good, pleasant aroma.
Comparative example 1
1. hypha fluid is prepared using method same as Example 1, then proceed to processing to prepare fermentation cordyceps
Cs-4 medicine materical crude slice, the process that this comparative example prepares medicine materical crude slice are as follows：
Hypha fluid is put into flame filter press in a manual manner, filter 23 hour under 0.35MPa；Then it incited somebody to action
It filters obtained mycelium and is put into drying in double-cone dryer, chuck pressure is 0.25MPa, and vacuum degree is -0.04MPa, and 65 DEG C dry
Dry 28 hours.After drying, room temperature crushing, mixed material, are packaged to be fermentation cordyceps Cs-4 medicine materical crude slice.Prepared by this law
Cordyceps particle is uneven, and the powder sieved by No. 7 only accounts for 50%；In addition, the material after dry obviously can see presence
The case where burning causes the color and luster of cordyceps Cs-4 medicine materical crude slice to differ, influences medical value.
Comparative example 2
2. hypha fluid is prepared using method same as Example 1, then proceed to processing to prepare fermentation cordyceps
Cs-4 medicine materical crude slice, the process that this comparative example prepares medicine materical crude slice are as follows：
Hypha fluid is put into flame filter press with pump, is filtered 5 hours under 0.30MPa；Then the sky of 0.1MPa is used
The hypha fluid of the dry moistening being obtained by filtration of air-blowing, blows 2 hours altogether.Next, the mycelium of drying is put into oven drying, very
Reciprocal of duty cycle is -0.04MPa, 90 DEG C of dryings 32 hours.After drying, room temperature crushing, mixed material, are packaged to be ferment cordyceps sinensis bacterium
Powder Cs-4 medicine materical crude slice.Cordyceps particle prepared by this law is uneven, the reason is that drying is insufficient, exists and assembles agglomerating phenomenon.
The color and luster for eventually leading to fermentation cordyceps Cs-4 medicine materical crude slice differs, and influences its medical value.
Comparative example 3
3. hypha fluid is prepared using method same as Example 1, then proceed to processing to prepare fermentation cordyceps
Cs-4 medicine materical crude slice, the process that this comparative example prepares medicine materical crude slice is similar to Example 4, but is formed without pill after first time is dry.
This comparative example is also differ in that with embodiment 4：Hypha fluid is put into flame filter press by the way of artificial,
Directly the mycelium of moistening is put into dry in double-cone dryer after filtering, drying temperature is 95 DEG C, vacuum degree is-
0.03MPa.Bipyramid after drying, stands 2 days, is then placed in oven drying, finally crushes, mixes, fermentation worm is made in packaging
Grass volvatus powder Cs-4 medicine materical crude slice.Cordyceps particle prepared by this law is uneven, exists and assembles agglomerating phenomenon, eventually leads to fermentation worm
The color and luster of grass volvatus powder Cs-4 medicine materical crude slice differs, and influences its medical value.
By the product for comparing the fermentation cordyceps Cs-4 medicine materical crude slice that above-described embodiment 2-4 and comparative example 1-3 are prepared
Character can be apparent from, and the product quality for the fermentation cordyceps Cs-4 medicine materical crude slice that the method for the present invention is prepared is more preferable.This hair
Bright method is easy to operate, is suitble to industrialized production, has very high practical value.
Embodiment 5
Nutrition analysis is carried out to fermentation cordyceps Cs-4 medicine materical crude slice prepared by embodiment 2-4, finds cordyceps Cs-4
Contain 19 kinds of amino acid in medicine materical crude slice, wherein seven kinds of essential amino acids that cannot be synthesized for human body itself, in addition, in bacterium powder medicine materical crude slice also
Contain potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, phosphorus, selenium and vitamin B1, B2 and vitamin E etc..Hair prepared by the method for the present invention
The amino acid contained in ferment cordyceps Cs-4 medicine materical crude slice is shown in Table 3.
| Amino acid | Mg/100mg samples | Amino acid | Mg/100mg samples |
| ASP asparatates | 2.13 | LEU leucines | 1.66 |
| THR threonines | 1.31 | TYR tyrosine | 0.80 |
| SER serines | 1.06 | PHE phenylalanines | 1.00 |
| GLU glutamic acid | 2.43 | LYS lysines | 1.38 |
| GLY glycine | 1.12 | NH3Ammonia | 0.44 |
| ALA alanine | 1.84 | HIS histidines | 0.49 |
| VAL valines | 1.33 | ARG arginine | 1.29 |
| MET methionine | 0.30 | PRO proline | 1.37 |
| ILE isoleucines | 1.22 | TRP tryptophans | 0.23 |
| CYS cystines | 0.30 | Summation | 21.70 |
Amino acid content in fermentation cordyceps Cs-4 medicine materical crude slice prepared by 3. the method for the present invention of table
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, the change or replacement that can be readily occurred in,
It should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice, which is characterized in that the preparation method comprises the following steps：
S1. strain culturing obtains qualified hypha fluid；
S2. the hypha fluid that collection step S1 is obtained, filtering, drying, obtains mycelium；
S3. the mycelium for using double-cone dryer drying steps S2 to obtain；
S4. pill is made in the step S3 mycelium dried；
S5. the pill that prepared by drying steps S4；
S6. the pill after pulverising step S5 dryings, obtains medicinal powder；
S7. mixed powder is packaged to be fermentation cordyceps Cs-4 medicine materical crude slice.
2. preparation method according to claim 1, which is characterized in that the strain culturing described in step S1 includes inclined-plane training
It supports, female bottle culture, seed tank culture, breeds tank culture and fermentation tank culture；Wherein, the nutrient media components of inclined-plane culture include：
Glucose, peptone, wheat bran, potassium dihydrogen phosphate, magnesium sulfate, agar and water；The nutrient media components of female bottle culture include：Grape
Sugar, peptone, wheat bran, potassium dihydrogen phosphate, magnesium sulfate and water.
3. preparation method according to claim 2, which is characterized in that seed tank culture, breeding tank culture and fermentation tank training
Foster nutrient media components include：Soybean cake powder, glucose, sucrose, soya-bean oil, potassium dihydrogen phosphate, magnesium sulfate and water.
4. preparation method according to claim 1, which is characterized in that filtering described in step S2 be selected from plate-frame filtering method,
One kind in high speed centrifugation method, bag filter process and pillar filtration method, preferably plate-frame filtering method；Filtration time is that 3-5 is small
When, pressure 0.25-0.35MPa.
5. according to claim 1-4 any one of them preparation methods, which is characterized in that double-cone dryer described in step S3 works
When chuck pressure 0.15-0.25MPa, vacuum degree is -0.05Mpa to -0.04Mpa, 50-90 DEG C of temperature, drying time 8-
18 hours.
6. preparation method according to claim 5, which is characterized in that the mycelial solid content that step S2 is obtained is 30-
40%.
7. preparation method according to claim 5, which is characterized in that the mycelial water content after the drying of step S3 bipyramids
Less than 55%.
8. preparation method according to claim 5, which is characterized in that the solid content of the pill after step S5 dryings is 85%
More than.
9. preparation method according to claim 5, which is characterized in that the drying described in step S5 is oven drying, step
Resting period of the pill made of S4 before entering oven drying is within 24 hours.
10. preparation method according to claim 5, which is characterized in that the drying described in step S2 is using pressure
The air of 0.1-0.2Mpa dries up 1-2 hours；The temperature crushed described in step S6 is 25-28 DEG C, is crushed 2 times altogether.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810512842.3A CN108553487A (en) | 2018-05-25 | 2018-05-25 | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice |
| PCT/CN2018/119030 WO2019223286A1 (en) | 2018-05-25 | 2018-12-04 | Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810512842.3A CN108553487A (en) | 2018-05-25 | 2018-05-25 | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108553487A true CN108553487A (en) | 2018-09-21 |
Family
ID=63539783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810512842.3A Pending CN108553487A (en) | 2018-05-25 | 2018-05-25 | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN108553487A (en) |
| WO (1) | WO2019223286A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019223286A1 (en) * | 2018-05-25 | 2019-11-28 | 江西国药有限责任公司 | Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces |
| CN113616681A (en) * | 2021-09-17 | 2021-11-09 | 江西国药有限责任公司 | Fermented cordyceps sinensis powder with high adenosine and high ergosterol and production method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1215754A (en) * | 1997-10-24 | 1999-05-05 | 中国医学科学院药物研究所 | Method for preparing fermentation product of cordyceps, and use thereof |
| CN101239080A (en) * | 2008-03-14 | 2008-08-13 | 杭州中美华东制药有限公司 | Extracting method for fermentation Chinese caterpillar fungus mycelium |
| CN103519329A (en) * | 2013-10-24 | 2014-01-22 | 江苏神华药业有限公司 | Drying system of fermented cordyceps sinensis mycelia and drying method of fermented cordyceps sinensis mycelia |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546644A (en) * | 2003-12-11 | 2004-11-17 | 孙悦迎 | Production method of Chinese caterpillar fungus powder and active Chinese caterpillar fungus polysaccharide freeze-drying powder for immunity, and product thereof |
| RO121677B1 (en) * | 2004-11-18 | 2008-02-28 | Marian Petre | Biotechnological process for preparing cordyceps sinensis (paecilomyces hepiali) fungal biomass having immunomodulating, antitumour and anti-infectious properties |
| CN101695509A (en) * | 2009-10-14 | 2010-04-21 | 香港理工大学深圳研究院 | Application of aweto cultured by artificial fermentation in preparing anti-fatigue and anti-hypoxia health product or drug |
| CN102783565B (en) * | 2012-08-10 | 2014-10-08 | 徐州瀚韵生物科技有限公司 | Preparation method of cordyceps feed additive |
| CN104643069B (en) * | 2015-01-31 | 2017-05-24 | 江苏神华药业有限公司 | Composite health composition and preparation method thereof |
| CN108057046A (en) * | 2017-12-26 | 2018-05-22 | 江西济民可信药业有限公司 | A kind of Jinshuibao piece preparation method using direct powder compression |
| CN108553487A (en) * | 2018-05-25 | 2018-09-21 | 江西国药有限责任公司 | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice |
-
2018
- 2018-05-25 CN CN201810512842.3A patent/CN108553487A/en active Pending
- 2018-12-04 WO PCT/CN2018/119030 patent/WO2019223286A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1215754A (en) * | 1997-10-24 | 1999-05-05 | 中国医学科学院药物研究所 | Method for preparing fermentation product of cordyceps, and use thereof |
| CN101239080A (en) * | 2008-03-14 | 2008-08-13 | 杭州中美华东制药有限公司 | Extracting method for fermentation Chinese caterpillar fungus mycelium |
| CN103519329A (en) * | 2013-10-24 | 2014-01-22 | 江苏神华药业有限公司 | Drying system of fermented cordyceps sinensis mycelia and drying method of fermented cordyceps sinensis mycelia |
Non-Patent Citations (3)
| Title |
|---|
| 刘广文: "《干燥设备选型及采购指南》", 30 September 2005, 中国石化出版社 * |
| 陆兆新: "《现代食品生物技术》", 31 August 2002, 中国农业出版社 * |
| 龚国光等: "双锥混合干燥机在发酵虫草菌粉提取中的使用及节能效应", 《江西能源》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019223286A1 (en) * | 2018-05-25 | 2019-11-28 | 江西国药有限责任公司 | Preparation method for fermented cordyceps sinensis powder cs-4 decoction pieces |
| CN113616681A (en) * | 2021-09-17 | 2021-11-09 | 江西国药有限责任公司 | Fermented cordyceps sinensis powder with high adenosine and high ergosterol and production method thereof |
| CN113616681B (en) * | 2021-09-17 | 2022-03-01 | 江西国药有限责任公司 | Fermented cordyceps sinensis powder with high adenosine and high ergosterol and production method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019223286A1 (en) | 2019-11-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101695257B (en) | Solid fermentation method for cordyceps sinensis | |
| CN103396956B (en) | A kind of saccharomyces cerevisiae and its screening and culturing method and the method for bean pulp fermentation | |
| CN102612985A (en) | Production technology for cordyceps militaris mycelium | |
| CN103060228B (en) | Lactobacillus plantarum and micro-ecological preparation for silage | |
| CN108220071A (en) | A kind of preparation method of hickory chick yellow rice wine | |
| CN103725714B (en) | A kind of hirsutella sinensis fungal powder production method | |
| CN102876587A (en) | Cordyceps militaris strain for producing cordycepin with high yield | |
| CN109355204A (en) | A kind of method of fermenting and producing cordyceps sinensis mycelium powder | |
| CN105981581B (en) | A kind of artificial culture method of cicada fungus | |
| CN101897270A (en) | Production technology of Cordyceps sinensis mycelium | |
| CN100383234C (en) | Bushi lactobacillus prepn., prepn. method and use thereof | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| CN103387428A (en) | Preparation method for organic material decomposition agent | |
| KR101138684B1 (en) | Method for culturing effective microorganisms and food using the same | |
| CN107586725B (en) | Cordyceps liquid culture medium and method for culturing cordyceps by using same | |
| CN108553487A (en) | A kind of preparation method of fermentation cordyceps Cs-4 medicine materical crude slice | |
| CN104082033A (en) | Ganoderma lucidum planting method | |
| CN103333846A (en) | Organic material decomposition agent | |
| KR20070008381A (en) | Method for culturing pleurotus eryngii including ginseng | |
| CN114058556B (en) | Preparation method of composite microbial agent | |
| CN110713956A (en) | Lysine bacillus S12 and application thereof | |
| KR100752335B1 (en) | Method for culturing sangwhangletari mushroom using phellinus linteus mycelium and letari mushroom, and the mushroom culture mat for sangwhangletari mushroom | |
| KR102316718B1 (en) | Manufacturing method for grains comprising the mycelium of mushrooms using a liquid medium | |
| CN102604840B (en) | Rice wine yeast, rhizopus and application thereof | |
| CN105441358A (en) | On-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180921 |