CN102160642B - Method for preparing Cordyceps rice food - Google Patents
Method for preparing Cordyceps rice food Download PDFInfo
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- CN102160642B CN102160642B CN2011100644594A CN201110064459A CN102160642B CN 102160642 B CN102160642 B CN 102160642B CN 2011100644594 A CN2011100644594 A CN 2011100644594A CN 201110064459 A CN201110064459 A CN 201110064459A CN 102160642 B CN102160642 B CN 102160642B
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Abstract
The invention discloses a method for preparing Cordyceps rice foods. The method has the advantages of short production cycle, stable quality of products and no pollution, and ensures that the requirements for food safety can be met. The method comprises the following specific steps: inoculating a Cordyceps liquid seed suspension which is obtained by virtue of liquid culture to rice which is swollen by water and sterilized; performing solid fermentation in a dark room and a light room alternately to produce Cordyceps-fermented rice; and further processing to produce the Cordyceps rice foods.
Description
Technical field
The present invention relates to a kind of method that adopts solid fermentation method to produce northern Chinese caterpillar fungus paddy food.
Background technology
Northern Chinese caterpillar Fungus (being called for short northern Chinese caterpillar fungus) is also named Cordyceps militaris, belongs to the Clavicipitaceae Cordyceps, belongs to xenogenesis together with Cordyceps sinensis, is a kind of medicinal fungi of preciousness.Chinese caterpillar fungus is lower for the requirement of growing environment in north; Liquid fermentation can form mycelium; Artificial extensive solid culture can obtain fructification; Medical value similar with the Cordyceps sinensis that has won fame both at home and abroad (Cordyceps sinensis polysaccharide of northern Chinese caterpillar fungus and cordycepic acid, suitable with the content of natural cordyceps, content of cordycepin is 35 times of natural cordyceps).Along with development of science and technology, people have reached brand-new stage to the development and use of northern Chinese caterpillar fungus, multiple northern Cordyceps occurred, and still, northern Chinese caterpillar Fungus should not arbitrarily add in food as medicinal material.
And along with growth in the living standard; People to the nutrition of food and function require increasingly high; Occur the paddy of composition such as many interpolations health care medicinal materials thus, the patent 200410015616.7 of University Of Science and Technology Of Tianjin provides a kind of solid state fermentation to produce the method for ferment rice bran, and edible bacteriums such as glossy ganoderma are inoculated in the rice bran; Process foods such as rice bran soymilk powder, thereby improve the nutritional labeling of rice bran; Patent 98103039.4 " preparation method of Chinese caterpillar fungus health food " also is that liquid spawn is inserted in rice or the wheat broth; But foregoing invention is not carried out strictness control to cultural hypha and seeded process, is difficult to satisfy the great attention of current society for food security.
Summary of the invention
The solid fermentation preparation method that the purpose of this invention is to provide a kind of northern Chinese caterpillar fungus paddy, the requirement of food security is satisfied in short production cycle, the constant product quality of this method, no any pollution.
Concrete technical scheme is following: will pass through the northern Chinese caterpillar fungus liquid seeds liquid of Liquid Culture, and be inoculated in the paddy that soaks, sterilizes, the solid fermentation method that adopts dark, bright chamber to replace prepares northern Chinese caterpillar fungus fermentation paddy.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 24～27 ℃ temperature, cultivated 3～6 days; Consisting of of its slant medium: dehydrated potato powder 13～18%, corn flour 0.5～9%, wheat bran 0～10%; Glucose 1～2.5%, peptone 0.2～0.5%, yeast extract 0.3～0.8%; Magnesium sulfate 0.1～0.3%, Cobastab
10～0.02%, agar 2.5～3%, skimmed milk power 1～2%, the pH value of control culture medium is 5～7.5, preferred 5～7, ideal situation is 6.5.
Discover that glucose is as the important component of growth, consumption should not surpass 2.5%, and the too high meeting of concentration of glucose causes the osmotic pressure of solution too high, and the moisture in the bacterial classification precipitate in the solution, causes bacterium cell dead; The present invention adopts dehydrated potato powder, corn flour, wheat bran and glucose that carbon source is provided, and milk powder and yeast extract replenish nitrogenous source, and the optimum content of carbon source, nitrogenous source is a dehydrated potato powder 14～16%; Corn flour 5～8%; Wheat bran 3～8%, glucose 2～2.5%, yeast extract 0.5～0.8%; Peptone is rich in amino acid, adds the energy for growth that peptone improves bacterial classification, and its content is preferably 0.4～0.5%.In addition, in culture medium, add Cobastab
1Can make the sturdy of mycelial growth, because Cobastab in the wheat bran
1Content is higher, as not adding wheat bran, can select to add Cobastab
10～0.02%; Vitamin E promotes growing of mycelia, also can add 0.02～0.04% vitamin E promotion bacterial classification growth in ideal conditions.
The growth of bacterial classification also needs mineral matter element, for example iron, magnesium, phosphorus, potassium, sodium, copper etc.The present invention adds the elements such as phosphorus, iron and other vitamin that the skimmed milk power that is rich in a large amount of trace elements provides growth to need, and its addition is 1～2%, is preferably 1～1.5%.
Temperature is for the bigger factor of the growth effect of bacterial classification, and generally speaking, temperature is high more, and mycelial growth is fast more, but growth should not store polysaccharide soon, does not advise that temperature is higher than 27 ℃, and under the lower situation of temperature, bacterial classification can stop growing.Therefore, suitable temperature is 24～27 ℃, and preferred optimum growth temp is 26.5～27 ℃.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24～27 ℃ temperature; Cultivated 5～10 days, and processed northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 18～22%; Glucose 1～2.5%; Skimmed milk power 0.1～0.5%, potassium dihydrogen phosphate 0.01～0.06%, magnesium sulfate 0～0.3%.
3, paddy and water were soaked in fermentation tank 2 hours according to the ratio of 2:1～6:1, then at 115～130 ℃ of temperature, 1.1Kg/cm
2Under the pressure, sterilized 15～30 minutes, wait to inoculate after being cooled to normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 10～22% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 5～10 days under 24～27 ℃ of conditions, under illumination condition, cultivated 10～20 days then, at room temperature carried out drying with natural wind at last, reached about 8-10% up to moisture.
5, further be processed into paddy food.
The northern Chinese caterpillar fungus liquid seeds liquid of cultivation and Liquid Culture through the inclined-plane is preferably received in the sterilization paddy by 15～20% inoculum concentration, alternately cultivates in darkroom and bright chamber, carries out solid fermentation.After the fermentation ends, carry out air dry, its moisture is reached about 9%.
Bacterial classification production of the present invention is carried out in strict accordance with the sterile working rules; The quality of culture medium raw material and additive detects through strict; The cereal that uses is through high-temperature sterilization, and sweat is got rid of assorted bacterium and noxious pollutant disturbs, thereby makes northern Chinese caterpillar fungus fermentation cereal possess good edible safety.
The specific embodiment
Embodiment 1
Preparing northern Chinese caterpillar fungus rice step through solid fermentation method describes.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 26.5～27 ℃ temperature, cultivated 3～6 days, the consisting of of its slant medium: dehydrated potato powder 15%; Corn flour 6%, wheat bran 3%, glucose 2.5%, peptone 0.3%; Yeast extract 0.8%, magnesium sulfate 0.1%, Cobastab
10.02%, agar 2%, skimmed milk power 2%, the pH value of control culture medium is 6.5.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24～27 ℃ temperature; Cultivated 7 days, and processed northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 22%; Glucose 2.5%; Skimmed milk power 0.5%, potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.3%.
3, rice and water were soaked in fermentation tank 2 hours according to the ratio of 5:1, then at 121 ℃ of temperature, 1.1Kg/cm
2Under the pressure, sterilized 30 minutes, wait to inoculate after being cooled to behind the normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 15% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 10 days under 24～27 ℃ of conditions, under illumination condition, cultivated 15 days then, at room temperature carried out drying with natural wind at last, made its moisture reach 10%.
Embodiment 2
Preparing northern Chinese caterpillar fungus rice bran step through solid fermentation method describes.
1, northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 26.5～27 ℃ temperature, cultivated 5 days, the consisting of of its slant medium: dehydrated potato powder 18%; Corn flour 0.5%, wheat bran 10%, glucose 2.5%, peptone 0.4%; Yeast extract 0.6%, magnesium sulfate 0.3%, vitamin E 0.02%; Agar 3%, skimmed milk power 2%, the pH value of control culture medium is 6.
2, the cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 25～26.5 ℃ temperature; Cultivated 7 days, and processed northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 22%; Glucose 2.5%; Skimmed milk power 0.5%, potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.3%.
3, rice bran and water were soaked in fermentation tank 2 hours according to the ratio of 6:1, then at 115 ℃ of temperature, 1.1Kg/cm
2Under the pressure, sterilized 30 minutes, wait to inoculate after being cooled to behind the normal temperature.
4, with cultured northern Chinese caterpillar fungus liquid seeds liquid, receive on the sterilization paddy by 22% inoculum concentration, carry out dark, the bright solid fermentation that replaces.Lucifuge was cultivated 10 days under 25～26.5 ℃ of conditions, under illumination condition, cultivated 15 days then, at room temperature carried out drying with natural wind at last, made its moisture reach 10%.
5, the further processing of northern Chinese caterpillar fungus rice bran is added in the cereal preparation.
Claims (1)
1. the preparation method of a northern Chinese caterpillar fungus paddy food, it adopts the solid fermentation method preparation, may further comprise the steps:
1) northern cordyceps species inclined-plane cultivation
Northern cordyceps species is inoculated on slant medium, under 24～27 ℃ temperature, cultivated 3～6 days; Consisting of of its slant medium: dehydrated potato powder 13～18%, corn flour 0.5～9%, wheat bran 0～10%; Glucose 1～2.5%, peptone 0.2～0.5%, yeast extract 0.3～0.8%; Magnesium sulfate 0.1～0.3%, Cobastab
10～0.02%, agar 1～3%, skimmed milk power 1～2%, the pH value of control culture medium is 5～7.5;
2) cultivation of northern Chinese caterpillar fungus liquid seeds liquid
Get a certain amount of slant medium and cultivate the northern cordyceps species of activation, receive fluid nutrient medium, under 24～27 ℃ temperature; Cultivated 5～10 days, and processed northern Chinese caterpillar fungus liquid spawn, the consisting of of fluid nutrient medium: potato 18～22%; Glucose 1～2.5%; Skimmed milk power 0.1～0.5%, potassium dihydrogen phosphate 0.01～0.06%, magnesium sulfate 0～0.3%;
3) paddy and water were soaked in fermentation tank 2 hours according to the ratio of 2:1～6:1, then at 115～130 ℃ of temperature, 1.1kg/cm
2Under the pressure, sterilized 15～30 minutes, wait to inoculate after being cooled to normal temperature;
4) with cultured northern Chinese caterpillar fungus liquid seeds liquid; Receive on the sterilization paddy by 10～22% inoculum concentration; Carry out dark, the bright solid fermentation that replaces: lucifuge was cultivated 5～10 days under 24～27 ℃ of conditions; Under illumination condition, cultivated 10～20 days then, at room temperature carry out drying at last, reach 8-10% up to moisture with natural wind.
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| CN2011100644594A CN102160642B (en) | 2011-03-17 | 2011-03-17 | Method for preparing Cordyceps rice food |
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| CN2011100644594A CN102160642B (en) | 2011-03-17 | 2011-03-17 | Method for preparing Cordyceps rice food |
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| CN102160642B true CN102160642B (en) | 2012-07-04 |
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| CN102388795B (en) * | 2011-11-08 | 2013-03-27 | 付湘晋 | Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds |
| CN102612984B (en) * | 2012-03-10 | 2013-09-04 | 何寒 | Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn |
| CN102613368A (en) * | 2012-04-24 | 2012-08-01 | 福建农大科技开发总公司 | North cordyceps sinensis herba epimedii compound tea bag product and producing method thereof |
| CN102823806B (en) * | 2012-09-20 | 2013-09-25 | 王永显 | Method for producing fermented oatmeal using Cordyceps militaris |
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| CN103960357A (en) * | 2013-02-06 | 2014-08-06 | 殷锋科 | Ecological transformation process for producing low-glycemic-index staple food by utilizing cordyceps militaris |
| CN103271281A (en) * | 2013-06-09 | 2013-09-04 | 食品行业生产力促进中心 | Method for producing cordyceps militaris fermentation coarse cereals through solid-state fermentation |
| CN103652627A (en) * | 2013-12-26 | 2014-03-26 | 大连盖世生物技术有限公司 | Cordyceps rice-flour noodle and processing method thereof |
| CN104718987A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis chenopodium quinoa |
| CN104824588A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis potatoes |
| CN104823691A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps corn |
| CN104686202A (en) * | 2015-03-20 | 2015-06-10 | 江南大学 | Cordyceps sinensis sweet potatoes |
| CN104824728A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis sesames |
| CN104824574A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis broad bean |
| CN104718989A (en) * | 2015-03-20 | 2015-06-24 | 江南大学 | Cordyceps sinensis dasheen |
| CN104824586A (en) * | 2015-03-20 | 2015-08-12 | 江南大学 | Cordyceps sinensis cassava |
| CN105053807A (en) * | 2015-08-18 | 2015-11-18 | 雷色香 | Chinese caterpillar fungus vermicelli |
| CN108371282B (en) * | 2016-10-12 | 2021-12-28 | 浙江泛亚保健食品有限公司 | Cordyceps sobolifera rice and preparation method thereof |
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| CN1208575A (en) * | 1998-07-21 | 1999-02-24 | 李健 | Method for preparing health-care food contg. Chinese caterpillar fungus |
| CN1650686A (en) * | 2005-01-06 | 2005-08-10 | 江苏省农业科学院 | Solid culturing method of north cateripillar fungus |
| CN101176521A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | North cordyceps rice flour product and preparation method thereof |
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- 2011-03-17 CN CN2011100644594A patent/CN102160642B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1208575A (en) * | 1998-07-21 | 1999-02-24 | 李健 | Method for preparing health-care food contg. Chinese caterpillar fungus |
| CN1650686A (en) * | 2005-01-06 | 2005-08-10 | 江苏省农业科学院 | Solid culturing method of north cateripillar fungus |
| CN101176521A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | North cordyceps rice flour product and preparation method thereof |
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