CN106259058A - A kind of fish and shrimp common disease Therapeutic Method with good result - Google Patents
A kind of fish and shrimp common disease Therapeutic Method with good result Download PDFInfo
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- CN106259058A CN106259058A CN201510264246.4A CN201510264246A CN106259058A CN 106259058 A CN106259058 A CN 106259058A CN 201510264246 A CN201510264246 A CN 201510264246A CN 106259058 A CN106259058 A CN 106259058A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 50
- 241000238557 Decapoda Species 0.000 title claims abstract description 41
- 201000010099 disease Diseases 0.000 title claims abstract description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 37
- 238000002560 therapeutic procedure Methods 0.000 title claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000003814 drug Substances 0.000 claims abstract description 23
- 150000003839 salts Chemical class 0.000 claims abstract description 13
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 12
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 10
- 239000011707 mineral Substances 0.000 claims abstract description 10
- IAIWVQXQOWNYOU-FPYGCLRLSA-N nitrofural Chemical compound NC(=O)N\N=C\C1=CC=C([N+]([O-])=O)O1 IAIWVQXQOWNYOU-FPYGCLRLSA-N 0.000 claims abstract description 5
- 235000013527 bean curd Nutrition 0.000 claims abstract description 4
- 229960000349 nitrofural Drugs 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 17
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000006260 foam Substances 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 claims description 4
- 229930182566 Gentamicin Natural products 0.000 claims description 4
- 239000004100 Oxytetracycline Substances 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- IWVCMVBTMGNXQD-PXOLEDIWSA-N oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 claims description 4
- 229960000625 oxytetracycline Drugs 0.000 claims description 4
- 235000019366 oxytetracycline Nutrition 0.000 claims description 4
- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 claims description 4
- 208000004232 Enteritis Diseases 0.000 claims description 3
- 206010020565 Hyperaemia Diseases 0.000 claims description 3
- 206010061218 Inflammation Diseases 0.000 claims description 3
- 230000003187 abdominal effect Effects 0.000 claims description 3
- 208000022531 anorexia Diseases 0.000 claims description 3
- 210000000436 anus Anatomy 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 239000012531 culture fluid Substances 0.000 claims description 3
- 206010061428 decreased appetite Diseases 0.000 claims description 3
- 208000010643 digestive system disease Diseases 0.000 claims description 3
- 208000018685 gastrointestinal system disease Diseases 0.000 claims description 3
- 230000004054 inflammatory process Effects 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 208000030194 mouth disease Diseases 0.000 claims description 3
- 230000003387 muscular Effects 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 229940079593 drug Drugs 0.000 abstract description 5
- 238000011534 incubation Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000012265 solid product Substances 0.000 abstract description 2
- 231100000033 toxigenic Toxicity 0.000 abstract description 2
- 230000001551 toxigenic effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 3
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 229960001907 nitrofurazone Drugs 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Farming Of Fish And Shellfish (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of fish and shrimp common disease Therapeutic Method with good result, and it concretely comprises the following steps: first carry out required medicament selection according to fish and shrimp disease, when fish, Testudinis bacillary beancurd sheet disease, utilizes nitrofural, salt to apply with the mass ratio of 2:3;The EM bacterium of the present invention is cultivated and utilizes Plastic Drum, graduated cylinder, mineral water bottle to cultivate, and Plastic Drum, graduated cylinder, mineral water bottle belong to recoverable device, have saved inventionculture tools, reduce toxigenic capacity.The present invention adds EM nitrobacteria in incubation, and the EM nitrobacteria property of medicine is long, joins in the cultivation of EM bacterium, extends the Types of Medicine of EM bacterium, need not constantly carry out EM bacterium interpolation, saved EM bacterium during use.EM bacterium in the present invention cultivates as solid product, it is to avoid the problem that liquid is difficult to place, preserve, it is simple to the use of user.
Description
Technical field
The present invention relates to raising fish and shrimp field, particularly relate to a kind of fish and shrimp common disease Therapeutic Method with good result.
Background technology
Fish and shrimp are in breeding process, and the easily various diseases of appearance, and during various disease treatment, effect of drugs is poor, and after treatment, fish and shrimp can be because the problems such as water quality occur that disease, fish and shrimp occur that disease is frequent again, ratio and the growth of fish and shrimp.The most typically utilize nitrofurazone powder, oxytetracycline powder, gentamycin etc. that fish and shrimp disease is treated, but these medication effects are poor, some needs use for a long time, have takes out fish and shrimp when needing to use, no matter but use for a long time or fish and shrimp are taken out, the most inconvenient, it is easily caused fish and shrimp during improper use in time dead.
EM bacterium is a kind of mixed vaccine, the microorganism formulation being typically made up of yeast, lactic acid bacteria and photosynthetic bacteria etc., can be used for food add,CultivationDisease control,SoilImprovement, strong plantlets and rootage, waste water control etc., EM bacterium can also be improved the immunity of fish and shrimp poultry, be treated fish and shrimp disease, hence it is evident that control harmful levels of pathogens, saves medicine spending.But EM bacterium cultural method is more complicated now, and EM bacterium now is generally liquid, uses, pack inconvenience, and EM bacterium Use Limitation now is short, needs commonly used to can be only achieved effect, does not utilize the treatment of fish and shrimp disease.
Summary of the invention
According to above technical problem, the present invention provides a kind of fish and shrimp common disease Therapeutic Method with good result, and it concretely comprises the following steps:
First carry out required medicament selection according to fish and shrimp disease, when fish, Testudinis bacillary beancurd sheet disease, utilize nitrofural, salt to apply with the mass ratio of 2:3;When fish and shrimp occur hoary hair, white mouth disease, muscular decay, anus redness, abdominal part enlargement, each fin hyperemia inflammation, enteritis, anorexia, do not like diseases such as moving about, oxytetracycline, salt is utilized to apply with the mass ratio of 1:1;When for fish and shrimp gastroenteropathy and fish gill disease protection with treatment time, utilize gentamycin, salt to apply with the mass ratio of 3:2;When treating the disease such as fish molds, ichthyophthiriasis, potassium permanganate, salt is utilized to apply with the mass ratio of 2:3;
Then during culturing jar put into by medicine, it is stirred, makes medicine uniformly melt, make medicine reach good result;
Finally EM bacterium is put in culturing jar, utilize EM bacterium to change water quality, while ensureing fish and shrimp disease treatment at all, also change the water quality of cultivation water, reduce the probability that fish and shrimp are ill, it is ensured that growing up healthy and sound of fish and shrimp.
A kind of EM bacterium cultural method, its concrete operation method is:
(1) cultivation instrument is prepared: between darkroom one, the Plastic Drum of 50L, graduated cylinder, mineral water bottle, PH reagent paper;(2) preparation cultivation raw material: EM strain, brown sugar, Mel, water, fermentation accelerant, EM nitrobacteria;(3) EM bacteria culture fluid preparation ratio is for EM strain 1-2: brown sugar 2-6: Mel 0.3-2: unboiled water 80-92:EM nitrobacteria 1-4;(4) technological process of production: first Plastic Drum, mineral water bottle, graduated cylinder are carried out sterilization process by (1), drying for standby after process;(2) in each bucket, put into brown sugar, pour clean unboiled water into, be subsequently adding EM strain and EM nitrobacteria, finally cover bung, it is not necessary to fully seal, in bucket is placed in darkroom, allow it ferment under semitight state;(3) room temperature is at 25 35 DEG C, and when fermentation proceeds to the 3rd day, the water surface there will be foam, at this moment needs to add fermentation accelerant, accelerates fermenting speed, promotes that beneficial microorganism generates;When the 8th day, foam slowly disappears, and the water surface floats the float of a piece of white;During by the l1 days, need to add Mel: during by the about l3 days, float sinks people's drum head, fermentation ends;
(4) filtering precipitate, the precipitate after filtering is dried, and packages standby after drying;(5) EM bacterium is between 25 35 DEG C, about l3 days;Between 15-25 DEG C, about l5 days, less than 15 DEG C need 17-19 days.
Described unboiled water can not be tap water.
The invention have the benefit that the present invention is in the method changing single common drug treatment, change the composition of medicine, and during the use of medicine, add the use of EM bacterium, cultivation water is changed while treatment disease, the replacing of cultivation water when avoiding disease treatment, while ensureing fish and shrimp disease treatment at all, also change the water quality of cultivation water, reduce the probability that fish and shrimp are ill, it is ensured that growing up healthy and sound of fish and shrimp.
The present invention, by using suitable ratio and unique fermentation technology, forms diversified microbe colony.EM bacterium of the present invention is cultivated whole flow processs and carries out in darkroom, it is to avoid irradiated by sunlight in incubation, it is ensured that the healthy growth of EM bacterium.The EM bacterium of the present invention is cultivated and utilizes Plastic Drum, graduated cylinder, mineral water bottle to cultivate, and Plastic Drum, graduated cylinder, mineral water bottle belong to recoverable device, have saved inventionculture tools, reduce toxigenic capacity.The present invention adds EM nitrobacteria in incubation, and the EM nitrobacteria property of medicine is long, joins in the cultivation of EM bacterium, extends the Types of Medicine of EM bacterium, need not constantly carry out EM bacterium interpolation, saved EM bacterium during use.EM bacterium in the present invention cultivates as solid product, it is to avoid the problem that liquid is difficult to place, preserve, it is simple to the use of user.
Detailed description of the invention
According to embodiment, the present invention is further described:
Embodiment 1
A kind of fish and shrimp common disease Therapeutic Method with good result, it concretely comprises the following steps:
First carry out required medicament selection according to fish and shrimp disease, when fish, Testudinis bacillary beancurd sheet disease, utilize nitrofural, salt to apply with the mass ratio of 2:3;When fish and shrimp occur hoary hair, white mouth disease, muscular decay, anus redness, abdominal part enlargement, each fin hyperemia inflammation, enteritis, anorexia, do not like diseases such as moving about, oxytetracycline, salt is utilized to apply with the mass ratio of 1:1;When for fish and shrimp gastroenteropathy and fish gill disease protection with treatment time, utilize gentamycin, salt to apply with the mass ratio of 3:2;When treating the disease such as fish molds, ichthyophthiriasis, potassium permanganate, salt is utilized to apply with the mass ratio of 2:3;Then during culturing jar put into by medicine, it is stirred, makes medicine uniformly melt, make medicine reach good result;Finally EM bacterium is put in culturing jar, utilize EM bacterium to change water quality, while ensureing fish and shrimp disease treatment at all, also change the water quality of cultivation water, reduce the probability that fish and shrimp are ill, it is ensured that growing up healthy and sound of fish and shrimp.
Embodiment 2
EM bacterium cultural method:
(1) cultivation instrument is prepared: between darkroom one, the Plastic Drum of 50L, graduated cylinder, mineral water bottle, PH reagent paper;(2) raw material: EM strain, brown sugar, Mel, water, fermentation accelerant, EM nitrobacteria.(3) EM bacteria culture fluid preparation ratio
EM bacterium: brown sugar: Mel: water: nitrobacteria is 2:4:1:90:3.(4) technological process of production: (1) by sterilized for the apparatus such as Plastic Drum, mineral water bottle, graduated cylinder disinfect standby;(2) in each bucket, 2 kilograms of brown sugar are put into, pour 45 kilograms of clean unboiled water (tap water should not be used to cultivate EM) into, it is subsequently adding the EM strain of l kilogram, the nitrobacteria of 1.5 kilograms, finally cover bung, need not fully seal, in bucket is placed in darkroom, allow it at semitight state bottom fermentation.(3) room temperature is at 25 35 DEG C, and when fermentation proceeds to the 3rd day, the water surface there will be foam, at this moment needs to add fermentation accelerant 50 grams, accelerates fermenting speed, promotes that beneficial microorganism generates;When the 8th day, foam slowly disappears, and the water surface floats the float of a piece of white;During by the l1 days, need to add the Mel of 0.5 kilogram: during by the about l3 days, float sinks people's drum head, fermentation ends.
(4) filtering precipitate, the precipitate after filtering is dried, and packages standby (5) EM bacterium after drying between 25 35 DEG C, about l3 days;Between 15-25 DEG C, about l5 days, less than 15 DEG C need 17-19 days, the EM bacterium of cultivation meets following provisions and can use.
The above is only the preferred embodiment of the present invention, it is noted that for those skilled in the art, under the premise without departing from the principles of the invention, it is also possible to make some improvement, and these improvement also should be regarded as protection scope of the present invention.
Claims (3)
1. having a fish and shrimp common disease Therapeutic Method for good result, it concretely comprises the following steps:
First carry out required medicament selection according to fish and shrimp disease, when fish, Testudinis bacillary beancurd sheet disease, utilize nitrofural, salt to apply with the mass ratio of 2:3;When fish and shrimp occur hoary hair, white mouth disease, muscular decay, anus redness, abdominal part enlargement, each fin hyperemia inflammation, enteritis, anorexia, do not like diseases such as moving about, oxytetracycline, salt is utilized to apply with the mass ratio of 1:1;When for fish and shrimp gastroenteropathy and fish gill disease protection with treatment time, utilize gentamycin, salt to apply with the mass ratio of 3:2;When treating the disease such as fish molds, ichthyophthiriasis, potassium permanganate, salt is utilized to apply with the mass ratio of 2:3;
Then during culturing jar put into by medicine, it is stirred, makes medicine uniformly melt, make medicine reach good result;
Finally EM bacterium is put in culturing jar, utilize EM bacterium to change water quality, while ensureing fish and shrimp disease treatment at all, also change the water quality of cultivation water, reduce the probability that fish and shrimp are ill, it is ensured that growing up healthy and sound of fish and shrimp.
2. an EM bacterium cultural method, its concrete operation method is:
(1) cultivation instrument is prepared: between darkroom one, the Plastic Drum of 50L, graduated cylinder, mineral water bottle, PH reagent paper;(2) preparation cultivation raw material: EM strain, brown sugar, Mel, water, fermentation accelerant;(3) EM bacteria culture fluid preparation ratio is for EM strain 1-2: brown sugar 2-6: Mel 0.3-2: unboiled water 80-92:EM nitrobacteria 1-4;
(4) technological process of production: first Plastic Drum, mineral water bottle, graduated cylinder are carried out sterilization process by (1), drying for standby after process;
(2) in each bucket, put into brown sugar, pour clean unboiled water into, be subsequently adding EM strain and EM nitrobacteria, finally cover bung, it is not necessary to fully seal, in bucket is placed in darkroom, allow it ferment under semitight state;(3) room temperature is at 25 35 DEG C, and when fermentation proceeds to the 3rd day, the water surface there will be foam, at this moment needs to add fermentation accelerant, accelerates fermenting speed, promotes that beneficial microorganism generates;When the 8th day, foam slowly disappears, and the water surface floats the float of a piece of white;During by the l1 days, Mel need to be added: during by the about l3 days, float sinks people's drum head, and then fermentation ends utilizes PH reagent paper to test result, determine cultivation results, when result meets the requirements, EM bacterium has been fermented, when result is undesirable, proceed fermentation, until result meets the requirements;(4) filtering precipitate, the precipitate after filtering is dried, and packages standby after drying;(5) EM bacterium is between 25 35 DEG C, about l3 days;Between 15-25 DEG C, about l5 days, less than 15 DEG C need 17-19 days.
3. according to a kind of EM bacterium cultural method described in claim 2, it is characterised in that described unboiled water can not be tap water.
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CN107278994A (en) * | 2017-06-13 | 2017-10-24 | 中国水产科学研究院黑龙江水产研究所 | One kind prevention fish oosperm saprolegniasis method |
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CN107278994A (en) * | 2017-06-13 | 2017-10-24 | 中国水产科学研究院黑龙江水产研究所 | One kind prevention fish oosperm saprolegniasis method |
CN108112787A (en) * | 2017-12-18 | 2018-06-05 | 自贡市轻工业设计研究院有限责任公司 | A kind of ornamental fish special-purpose salt and preparation method thereof |
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