CN101558820A - Production method for bioprotein feeding stuff - Google Patents
Production method for bioprotein feeding stuff Download PDFInfo
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- CN101558820A CN101558820A CNA2009100592003A CN200910059200A CN101558820A CN 101558820 A CN101558820 A CN 101558820A CN A2009100592003 A CNA2009100592003 A CN A2009100592003A CN 200910059200 A CN200910059200 A CN 200910059200A CN 101558820 A CN101558820 A CN 101558820A
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Abstract
The invention provides a production method for bioprotein feeding stuff, which relates to the field of feeding stuff production, and aims to solve the technical problems of badly needed conventional protein feeding stuff, quite imbalanced nutrient contents, low utilization coefficient, harm to human health done by antibiotics used in the animal feeding stuff, and the like. The method comprises the following steps: respectively preparing a lactobacillus culture medium, a microzyme culture medium and a bacillus culture medium; respectively inoculating lactobacillus, microzyme and bacillus after germicidal treatment and cooling; saccharifying the three culture solutions, and introducing the culture solutions into a chemostat for chemostatic treatment; diluting the three bacteria solutions after the chemostatic treatment with water, mixing the three diluted solution with crushed solid feeding stuff, and carrying out inoculation, fermentation and low temperature drying on the mixture. The method is suitable for producing the protein feeding stuff.
Description
Technical field
The present invention relates to the biological feedstuff production field, particularly the production method of biotinylated biomolecule protein feed.
Background technology
According to relevant, developing country mostly is an agriculture country, and the agriculture output value accounts for significant proportion in these national economies.Making one of effective way that the agriculture output value increases substantially is exactly develop actively animal husbandry, and the primary problem that solves of developing animal husbandry is exactly a feed, solving the feed best bet is exactly a large amount of plantations, and another way is exactly to expand particularly protein feed source of other feed energetically.In the face of huge population growth's pressure, grain, particularly protein requirement contradiction shows especially, and increasing along with population, resource, environmental pressure, these processing industries are being faced with great challenge.The protein feeds of using as feed is more in short supply, and countries in the world are all striving to find solution route.China asks an exorbitant fare to external import dregs of beans more than 2,000 ten thousand tons every year, and China will produce every year such as the dish dregs of rice, cotton dregs etc. more than 1,500 ten thousand tons, price has only half of import dregs of beans, but because ANFs content height, concentrations of nutrient is reason such as imbalance extremely, can only adopt the method for restricted scale of rationing, utilization rate is less than half, most of being wasted.Even to this day, conventional art can not significantly improve the production performance of animal again, and popularizing of routine techniques also makes each company's product be tending towards homogeneity, and the competition of quality turns on the competition of price, how promoting the scientific and technological content of raw material variety, is present problem demanding prompt solution.
On the other hand, today, people put health in the first place, and feed is considered as human indirect food, and feed safety promptly is a food security.A large amount of to use the caused resistances to the action of a drug and medicament residue problem then be a very big potential safety hazard to antibiotic in drug resistance that the antibiotic a large amount of uses of medical industry produce and feed.With regard to human, kinds of Diseases increase day by day, and antibiotic dosage also increases thereupon, how to strengthen immunity of organisms, is the problem that present nutritionist and Medical Technologist day and night study.With regard to aquaculture, the breed characteristics in Sichuan are exactly that quantity is big, small scale, disperse to raise, and the level of production is low, and health can not get ensureing.And take the simplest also be that the most effective counter-measure is exactly a large amount of antibiotic that add in feed, the immunity of animal descends gradually, disease is in case outburst, even common streptococcus is also restive.So consider that from public health, food security and international trade barrier equal angles China can forbid antibiotic use in the feed sooner or later.But the experience of European Union is told us, and the forbidding of feeding antibiotic can cause breeding performonce fo animals decline 10-15%, and according to the national conditions of China, antibiotic forbidding may cause China's herding level of production to fall back more than 10 years.How to improve human health and reduce antibiotic application and how to accomplish not use in the feed antibiotic can guarantee that again the production performance of animal does not descend, traditional food industry technology and feed processing preparation technique are powerless.
Summary of the invention
The present invention is intended to solve that traditional protein feeds is in short supply, nutrition content is extremely uneven, utilization rate is low and use antibiotic to bring many technical problems such as infringement to human health in animal feed, and a kind of production cost is low to provide, high production, nutrition content height, utilization rate height, do not add antibiotic protein feeds.
The objective of the invention is to be achieved through the following technical solutions.
The production method of biological protein feedstuff of the present invention comprises following steps:
A) difference making lactic acid bacillus, saccharomycete, bacillus culture medium;
B) to above-mentioned culture medium under 120 ℃ ± 25 ℃ temperature conditions, sterilization treatment 15～30 minutes;
C) inoculating lactobacillus, saccharomycete, bacillus obtain elementary nutrient solution in culture medium separately respectively cooling back;
D) repeating step a)～b), pour into three kinds of culture mediums after sterilization treatment in the tubualted bottle respectively, wherein the tubualted bottle of splendid attire saccharomycete and bacillus is equipped with logical oxygen device, uses above-mentioned steps c afterwards respectively) in the respective primary nutrient solution that makes inoculate, obtain nutrient solution.
E) three kinds of nutrient solutions that will be obtained by step d) are after saccharification is handled, and insulation was introduced chemostat and carried out the perseverance processing more than 1 hour;
F) three kinds of bacterium liquid that will be after the perseverance processing and water are respectively by 3～6: 50～70 dilutions, and with three kinds of dilutions and after crushed solid feed combined inoculation, inoculative proportion material bacterium is than 1: 0.5 ± 0.3 afterwards;
G) will be through the solid feed after the step f) combined inoculation ferment under aerobic conditions 48 ± 24 hours, afterwards with its low temperature drying.
The production method of aforementioned biological protein feedstuff, three kinds of culture mediums in the wherein said step a) contain the component of following weight portion meter respectively
A) Bacillus acidi lactici culture medium
Beef leaching thing 3.0-5.0
Yeast extract 5.0-8.0
Glucose 3.0-5.0
Sodium acetate 4.0-6.0
Citric acid diamines 1.0-2.0
Dipotassium hydrogen phosphate 1.0-2.0
Four water magnesium sulfate 0.2-0.4
Seven water manganese sulfate 0.05-0.06
Distilled water 1.0
B) microzyme culture medium:
Moyashi 80-90
Glucose 30-50
Agar 10-15
Distilled water 1.0
C) bacillus culture medium:
Glucose 20-30
Peptone 5-8
Sodium chloride 3-5
Beef extract 0.5-0.8
Distilled water 1.0.
The production method of aforementioned biological protein feedstuff, in wherein said step c) and the step d) respectively inoculating lactobacillus, saccharomycete, bacillus in culture medium and elementary nutrient solution separately, wherein Bacillus acidi lactici is placed in the CO2gas incubator and is left standstill, and cultivates 24h ± 12h for 37 ℃ ± 5 ℃; Saccharomycete is placed 32 ℃ ± 5 ℃ and is cultivated 24h ± 12h; Bacillus is placed 37 ℃ ± 5 ℃ and cultivates 24h ± 12h.
The production method of aforementioned biological protein feedstuff, the perseverance processing in the wherein said step e) comprises following steps
A) by weight Bacillus acidi lactici, saccharomycete, bacillus 20 ± 10 of inoculation respectively in three chemostats, 50 ± 30/ hours flow velocity by weight adds saccharification liquid, add 6 ± 3 hours continuously, close the stream adding system, stir culture 24h ± 12h reaches viable count: Bacillus acidi lactici 〉=4 ± 2 * 10
9CFU/ml; Saccharomycete 〉=5 ± 3 * 10
8CFU/ml; Bacillus 〉=8 ± 3 * 10
8CFU/ml;
B) by weight Bacillus acidi lactici, saccharomycete, bacillus 20 ± 10 of inoculation respectively in above-mentioned three chemostats, 50 ± 30/ hours flow velocity by weight adds saccharification liquid, adds 9 ± 3 hours continuously, closes the stream adding system, stir culture 24h ± 12h, 24h ± 12h to be cultivated.
The production method of aforementioned biological protein feedstuff, the solid feed in the wherein said step g) comprises following steps at ferment under aerobic conditions
A) fermentation is after 12 ± 5 hours, and turning once and is measured 38 ℃ ± 12 ℃ of temperature;
B) once every turning in 4 hours.
The beneficial effect of the production method of biological protein feedstuff of the present invention:
1. low-cost, high production;
2. nutrition content height, the ratio balance;
3. utilization rate height;
4. do not add antibiotic.
Description of drawings
Fig. 1 is the production method batching inoculating process schematic flow sheet of biological protein feedstuff of the present invention
Fig. 2 is the production method breeding technique schematic flow sheet of biological protein feedstuff of the present invention
Fig. 3 is the logical oxygen device schematic diagram of end opening in the production method of biological protein feedstuff of the present invention
The specific embodiment
Application principle of the present invention, effect and effect are illustrated by following embodiment with reference to accompanying drawing 1-2.
Embodiment 1
One, the laboratory bacterial classification is cultivated
1. the elementary cultivation of bacterial classification
1) prepares the following culture medium of 1L respectively
Bacillus acidi lactici culture medium (g)
Beef leaching thing 5.0
Yeast extract 5.0
Glucose 5.0
Sodium acetate 5.0
Citric acid diamines 2.0
Dipotassium hydrogen phosphate 2.0
Four water magnesium sulfates 0.2
Seven water manganese sulfates 0.05
Distilled water 1.0L
Microzyme culture medium (g):
Moyashi 90
Glucose 50
Agar 15
Distilled water 1.0L
Bacillus culture medium (g):
Glucose 20
Peptone 5
Sodium chloride 5
Beef extract 0.5
Distilled water 1.0L
2) sterilization
After preparing above culture medium, use the conical flask packing, mark, was sterilized 20 minutes by 121 ℃-125 ℃.
3) inoculation and cultivation
After the cooling, respectively inoculating lactobacillus, saccharomycete, bacillus are in culture medium separately in sterile working, and wherein, Bacillus acidi lactici is placed in the carbonoxide incubator and left standstill, and cultivates 24h ± 12h for 37 ℃ ± 5 ℃; Saccharomycete is placed 32 ℃ ± 5 ℃ and is cultivated 24h ± 12h; Bacillus is placed 37 ℃ ± 5 ℃ and cultivates 24h ± 12h.Obtain elementary nutrient solution.
2. the preparation of secondary seed medium and cultivation
Prepare the above culture medium of 10L respectively.121 ℃ ± 25 ℃, sterilized 20 minutes.In the desinfection chamber cooled culture medium poured in advance cleaned 10L tubualted bottle (wherein, the tubualted bottle of dress saccharomycete and bacillus should be adorned logical oxygen device, sees Fig. 3) respectively.With above-mentioned cultured elementary nutrient solution culture medium is inoculated respectively.Bacillus acidi lactici is placed in the CO2gas incubator and leaves standstill, and cultivates 24h ± 12h for 37 ℃ ± 5 ℃; Saccharomycete is placed 32 ℃ ± 5 ℃ and is cultivated 24h ± 12h; Bacillus is placed 37 ℃ ± 5 ℃ and cultivates 24h ± 12h.Obtain three kinds of nutrient solutions.
3. viable count (blood count colony counting method)
Bacillus acidi lactici 〉=2 * 10
9CFU/ml;
Saccharomycete 〉=5 * 10
8CFU/ml;
Bacillus 〉=8 * 10
8CFU/ml.
Two, breeding Workshop Production technological operation flow process
1. material-compound tank → saccharifying tank:
1., open the material-compound tank running water valve, inject running water, start when treating water level to stir button and in tank body, add and prepare burden etc. for tank body 1/2-2/3 place.
2., when the water level canful, close the running water water injection valve, treat that feed liquid is fully diluted mixing after, open the feed pump switch and pump feed liquid to saccharifying tank.
3., open the running water water injection valve, stir button when water level starts during greater than 3000L, and water level added to produce need the site.
4., open Steam Heating valve intensification carrying out saccharification.
2. saccharifying tank → insulation jar:
1., open feed liquid input valve, open the saccharifying tank feed pump simultaneously, regulate the flow in the glass ear of maize flow, be adjusted to temperature stabilization at 100 ± 20 ℃.
2., the reduction of feed volume in insulation jar is during greater than 3000L, opens an insulation jar stirring pump.More than the insulation duration 1h.
3. be incubated jar → chemostat:
Open the cooling water Water filling valve, open insulation jar fluid valve then, simultaneously, start the reinforced constant current control button of chemostat.After feed liquid is flowed through plate type heat exchanger, open the by-pass valve switch, discharge air to feed liquid benefit, valve-off are then arranged.
When taking out feed liquid, the valve of opening split flow pump drains air and restarts the reinforced constant current control button of chemostat.
4. chemostat production operation rules
1., inoculation.In three chemostats, meet bacterial classification 20 ± 10L, open Constant Current Control System then, the flow velocity of pressing 50L ± 30L/ hour adds saccharification liquid, add continuously about 6 ± 3 hours, close the stream adding system, start the stirring system button and stir, cultivate 24h ± 12h, cultivate to reach and produce that to need viable count be Bacillus acidi lactici 〉=4 ± 2 * 109CFU/ml; Saccharomycete 〉=5 ± 3 * 108CFU/ml; Bacillus 〉=8 ± 3 * 108CFU/ml.
2., met bacterial classification 20 ± 10L in second day, open Constant Current Control System then, the flow velocity of pressing 50L ± 30L/ hour adds saccharification liquid, adds continuously about 9 ± 3 hours, closes the stream adding system, starts the stirring system button and stirs, and cultivates 24h ± 12h.Carry out solid vaccination behind 24h ± 12h to be cultivated.
3., jede Woche replenishes 1-2 bacterial classification during ordinary production, carry out flame after connecing the bacterium mouth and inoculate connecing to clean with distilled water before and after the bacterium.
5. chemostat → blending tank:
1., regulate the digital display flow quantity control instrument, configure the amount of the zymotic fluid that flows out from each chemostat the inside.Indicator is pointed to No. 1 chemostat.
2., open the feed liquid output valve of No. 1 chemostat, open No. 1 defeated material of chemostat kinetic pump switch on the digital display flow quantity control instrument.After pumping and finish, feed liquid closes the feed liquid output valve of No. 1 chemostat.
3., open the feed liquid output valve of No. 2 chemostats, open No. 1 defeated material of chemostat kinetic pump switch on the digital display flow quantity control instrument.After pumping and finish, feed liquid closes the feed liquid output valve of No. 2 chemostats.
4., open the feed liquid output valve of No. 3 chemostats, open No. 1 defeated material of chemostat kinetic pump switch on the digital display flow quantity control instrument.After pumping and finish, feed liquid closes the feed liquid output valve of No. 3 chemostats.
5., three kinds of bacterium liquid respectively is 6: 60 with the ratio row of water.
6. blending tank discharges bacterium dilution at night:
Start arrow formula vaccinization machine and conveying worm button, make its running 1., earlier.
2., on the constant current controller, set needed flow, start the Mixed Microbes constant flow pump synchronously with solid material.
Three, raw material premix Workshop Production technology
1. the screening raw material is pulverized, and grinding particle size is at 20 ± 10 orders, after rise to storehouse to be mixed;
2. set the different proportion of each raw material: after 5%, 10%, 20%, 30%, 50%, enter the automatic material weighing system;
3. the raw material that weighs up enters mixer by elevator to be mixed and enters arrow formula vaccinization machine after 5 ± 3 minutes, and inoculative proportion material bacterium is than 1: 0.5 ± 0.3.
Four, solid material fermentation
1. fermentation plant cleans and sterilizes, and thimerosal according to corresponding better than the row dilution, is washed multipass with giant, rinses well with clear water again, treats that the irradiation of the clean back unlatching in ground ultraviolet lamp is more than 30 minutes.
2. cloth: the material that bacterial classification inoculation is good, insert fermentation tank by belt conveyor, treat that all material cloth are good after, unlatching aerobic turning machine is with the material turning once.
3. after fermenting 12 ± 5 hours, turning once and is measured 38 ℃ ± 12 ℃ of temperature, after this, and every turning in 4 hours once.Ferment and after 48 ± 24 hours material is dried.
Five, oven dry
Adopt the tube bank low temperature drying, contact temperature of charge≤80 ℃, finished product temperature≤35 ℃.
One, the laboratory bacterial classification is cultivated
1. the elementary cultivation of bacterial classification
1) prepares the following culture medium of 1L respectively
Bacillus acidi lactici culture medium (g)
Beef leaching thing 3.0
Yeast extract 5.0
Glucose 3.0
Sodium acetate 4.0
Citric acid diamines 1.0
Dipotassium hydrogen phosphate 1.0
Four water magnesium sulfates 0.2
Seven water manganese sulfates 0.05
Distilled water 1.0L
Microzyme culture medium (g):
Moyashi 80
Glucose 30
Agar 10
Distilled water 1.0L
Bacillus culture medium (g):
Glucose 20
Peptone 5
Sodium chloride 3
Beef extract 0.5
Distilled water 1.0L
Following steps are with embodiment 1
Embodiment 3
One, the laboratory bacterial classification is cultivated
1. the elementary cultivation of bacterial classification
1) prepares the following culture medium of 1L respectively
Bacillus acidi lactici culture medium (g)
Beef leaching thing 4.0
Yeast extract 60
Glucose 4.0
Sodium acetate 5.0
Citric acid diamines 1.5
Dipotassium hydrogen phosphate 1.5
Four water magnesium sulfates 0.3
Seven water manganese sulfates 0.055
Distilled water 1.0L
Microzyme culture medium (g):
Moyashi 85
Glucose 40
Agar 13
Distilled water 1.0L
Bacillus culture medium (g):
Glucose 25
Peptone 6
Sodium chloride 4
Beef extract 0.6
Distilled water 1.0L
Following steps are with embodiment 1
From the above, the production method of biological protein feedstuff of the present invention has and produces low cost, high production, nutriment and contain Amount is high, utilization rate is high, do not add the plurality of advantages such as antibiotic.
Claims (5)
1. the production method of a biological protein feedstuff is characterized in that comprising following steps:
A) difference making lactic acid bacillus, saccharomycete, bacillus culture medium;
B) to above-mentioned culture medium under 120 ℃ ± 25 ℃ temperature conditions, sterilization treatment 15～30 minutes;
C) inoculating lactobacillus, saccharomycete, bacillus subtilis obtain elementary nutrient solution in culture medium separately respectively cooling back;
D) repeating step a)～b), pour into three kinds of culture mediums after sterilization treatment in the tubualted bottle respectively, wherein the tubualted bottle of splendid attire saccharomycete and bacillus is equipped with logical oxygen device, uses above-mentioned steps c afterwards respectively) in the respective primary nutrient solution that makes inoculate, obtain nutrient solution.
E) three kinds of nutrient solutions that will be obtained by step d) are after saccharification is handled, and insulation was introduced chemostat and carried out the perseverance processing more than 1 hour;
F) three kinds of bacterium liquid that will be after the perseverance processing and water are respectively by 3～6: 50～70 dilutions, and with three kinds of dilutions and after crushed solid feed combined inoculation, inoculative proportion material bacterium is than 1: 0.5 ± 0.3 afterwards;
G) will be through the solid feed after the step f) combined inoculation ferment under aerobic conditions 48 ± 24 hours, afterwards with its low temperature drying.
2. the production method of biological protein feedstuff according to claim 1, it is characterized in that: three kinds of culture mediums in the described step a) contain the component of following weight portion meter respectively
A) Bacillus acidi lactici culture medium:
Beef leaching thing 3.0-5.0
Yeast extract 5.0-8.0
Glucose 3.0-5.0
Sodium acetate 4.0-6.0
Citric acid diamines 1.0-2.0
Dipotassium hydrogen phosphate 1.0-2.0
Four water magnesium sulfate 0.2-0.4
Seven water manganese sulfate 0.05-0.06
Distilled water 1.0
B) microzyme culture medium:
Moyashi 80-90
Glucose 30-50
Agar 10-15
Distilled water 1.0
C) bacillus culture medium:
Glucose 20-30
Peptone 5-8
Sodium chloride 3-5
Beef extract 0.5-0.8
Distilled water 1.0.
3. the production method of biological protein feedstuff according to claim 1, it is characterized in that: in described step c) and the step d) respectively inoculating lactobacillus, saccharomycete, bacillus in culture medium and elementary nutrient solution separately, wherein Bacillus acidi lactici is placed in the CO2gas incubator and is left standstill, and cultivates 24h ± 12h for 37 ℃ ± 5 ℃; Saccharomycete is placed 32 ℃ ± 5 ℃ and is cultivated 24h ± 12h; Bacillus is placed 37 ℃ ± 5 ℃ and cultivates 24h ± 12h.
4. the production method of biological protein feedstuff according to claim 1, it is characterized in that: the perseverance processing in the described step e) comprises following steps
A) by weight Bacillus acidi lactici, saccharomycete, bacillus 20 ± 10 of inoculation respectively in three chemostats, 50 ± 30/ hours flow velocity by weight adds saccharification liquid, add 6 ± 3 hours continuously, close the stream adding system, stir culture 24h ± 12h reaches viable count: Bacillus acidi lactici 〉=4 ± 2 * 109CFU/ml; Saccharomycete 〉=5 ± 3 * 108CFU/ml bacillus 〉=8 ± 3 * 108CFU/ml
B) by weight Bacillus acidi lactici, saccharomycete, bacillus 20 ± 10 of inoculation respectively in above-mentioned three chemostats, 50 ± 30/ hours flow velocity by weight adds saccharification liquid, adds 9 ± 3 hours continuously, closes the stream adding system, stir culture 24h ± 12h, 24h ± 12h to be cultivated.
5. the production method of biological protein feedstuff according to claim 1, it is characterized in that: the solid feed in the described step g) comprises following steps at ferment under aerobic conditions
A) fermentation is after 12 ± 5 hours, and turning once and is measured 38 ℃ ± 12 ℃ of temperature;
B) once every turning in 4 hours.
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|---|---|---|---|
| CNA2009100592003A CN101558820A (en) | 2009-05-07 | 2009-05-07 | Production method for bioprotein feeding stuff |
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|---|---|---|---|
| CNA2009100592003A CN101558820A (en) | 2009-05-07 | 2009-05-07 | Production method for bioprotein feeding stuff |
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| CN103211102A (en) * | 2013-05-16 | 2013-07-24 | 徐静 | Feed bionic acid processing and producing method |
| CN106538824A (en) * | 2016-09-26 | 2017-03-29 | 广州好师傅生物科技有限公司 | A kind of polypeptide type feed addictive and preparation method thereof |
| CN107646525A (en) * | 2017-10-31 | 2018-02-02 | 翔天农业开发集团股份有限公司 | A kind of liquid spawn fermentation tank pH stable method and its device |
| CN110720548A (en) * | 2019-11-22 | 2020-01-24 | 安徽五粮泰生物工程股份有限公司 | Clean production method of high-content yeast protein feed |
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Application publication date: 20091021 |