CN101603016A - Live lactobacillus composite ecological microbial agent - Google Patents
Live lactobacillus composite ecological microbial agent Download PDFInfo
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- CN101603016A CN101603016A CNA2009100123316A CN200910012331A CN101603016A CN 101603016 A CN101603016 A CN 101603016A CN A2009100123316 A CNA2009100123316 A CN A2009100123316A CN 200910012331 A CN200910012331 A CN 200910012331A CN 101603016 A CN101603016 A CN 101603016A
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Abstract
The present invention relates to bio-pharmaceuticals and multifunctional ecological goods.Live lactobacillus composite ecological microbial agent, it is to be cultivated through ferment tank by the probiotic bacterium that is representative to form with live lactobacillus and yeast, other live probiotics bacterial classifications are selected from nitrobacteria, sulphur bacteria, one or more in photosynthetic bacterium and the genus bacillus.The scope of application of the present invention is extensive: as raising pigs, raise chickens cowboying as livestock-raising, support poultry men such as animal, livestock culturing industry, or used for aquiculture such as breed fish, have all multi-functional: can improve immunizing power behind the animal edible, reduce and use microbiotic, save the drug use cost; Obviously improve breeding potential, shorten breeding cycle.Obvious and effective is saved feed, improves economic income of supporting the family.
Description
Technical field:
The present invention relates to bio-pharmaceuticals and multifunctional ecological goods, particularly composite ecological microbial agent.
Background technology:
At present countries in the world, especially developed countries such as Europe, Japan have realized that microbiotic to human body and ecological undesirable action, in the strict restriction in aspect microbiotic use such as feeding, medicinal.And the minimizing of antibiotic dosage or do not use, bigger for influences such as feeding animals or plantation plant survival rate, yields, therefore people's development research can substitute microbiotic, antibacterials, but have no side effect again, the probiotics of noresidue, this moment, ecological microbial agent arose at the historic moment, and was applied in the every field by general.
Ecological microbial agent is divided into single microbial inoculum and composite fungus agent at present.Single microbial inoculum is the preparation that only is made of a certain microorganism, as agent of lactic acid bacteria; Composite fungus agent is the preparation that is made of two or more microorganism, as genus bacillus and lactobacillus composite fungus agent, has synergy, genus bacillus is a height oxygen consumption bacterium, anaerobic environment can effectively suppress the pathogenic bacterium growth in the organizer, simultaneously help the anaerobic lactobacter growth again, lactobacter growth has increased the acidity of environment, further strengthens the restraining effect to pathogenic bacterium.But it is higher that weak point is present composite fungus agent price, and composition is too simple, and addition is not very low, makes the use cost height.Do not act synergistically between the bacterial classification, spawn activity is not strong, can not bring abundant income in the provisions family though produce effect.
Summary of the invention:
The objective of the invention is to address the above problem, a kind of live lactobacillus composite ecological microbial agent is provided, the bacterial classification that composite fungus agent adopted is reasonable, spawn activity is strong, and synergy significantly adopts modernized fermentor tank production between bacterial classification, reduce production costs and use cost, be widely used, effect is remarkable.
The technical scheme that the present invention is adopted for achieving the above object is: live lactobacillus composite ecological microbial agent, it is to be cultivated through ferment tank by the probiotic bacterium that is representative to form with live lactobacillus and yeast, other live probiotics bacterial classifications are selected from nitrobacteria, sulphur bacteria, one or more in photosynthetic bacterium and the genus bacillus.
Described ecological microbial agent is cultivated through ferment tank by live lactobacillus, yeast, nitrobacteria, sulphur bacteria, photosynthetic bacterium and probiotic bacillus group and is formed.
Every milligram of bacterium several 1 * 10 that contains live lactobacillus of described ecological microbial agent
4-1 * 10
10, saccharomycetic viable count 1 * 10
3-1 * 10
10
The bacterium number of described other live probiotics nitrobacterias, sulphur bacteria, photosynthetic bacterium and genus bacillus is no more than 1 * 10 respectively
1
Described yeast comprises yeast saccharomyces cerevisiae, saccharomyces fragilis and Candida arborea.Described milk-acid bacteria comprises lactobacillus and lactic acid coccus, and lactobacillus comprises lactobacterium casei, plant lactobacillus, Lactobacterium acidophilum and lactobacillus lactis; The lactic acid coccus comprises Streptococcus cremoris, thermophilus streptococcus, Leuconostoc mesenteroides and enterococcus faecalis.Described genus bacillus comprises subtilis, bacillus mesentericus and butyric acid rib bacterium; Nitrobacteria is adopted the Vickers bacterium nitrobacter; Sulphur bacteria is adopted thiobacillus; Photosynthetic bacterium is a photosynthetic bacterium.
The method for making of ecological microbial agent of the present invention, concrete technology: in fermentor tank, put into pure water, add entry gross weight 3-5% glucose and water gross weight 0.01-0.03% albumen, keep the 100-120 degree after the heat, be incubated 15-20 minute, be cooled to room temperature then, press the inoculation of 1-5% concentration, little oxygen stir culture 3-5 days, anaerobic was cultivated 20-24 hour, pH value is reduced between the 3.0-4.5, and every milligram contains live lactobacillus bacterium several 1 * 10
4-1 * 10
10, saccharomycetic viable count is 1 * 10
3-1 * 10
10, go out jar under the room temperature, promptly make live lactobacillus composite ecological microbial agent by the metering packing.
The scope of application of the present invention is extensive: as raising pigs, raise chickens cowboying as livestock-raising, support poultry men such as animal, livestock culturing industry, or used for aquiculture such as breed fish, have all multi-functional: can improve immunizing power behind the animal edible, reduce and use microbiotic, save the drug use cost; Obviously improve breeding potential, shorten breeding cycle.Obvious and effective is saved feed, improves economic income of supporting the family.Can decompose harmful organic substances in feed and the animal body, improve the quality of fishery products and meat, milk, egg.The diarrhoea that significant effective control intestinal bacteria cause stops just material; Can reduce feedstuff-meat ratio significantly, improve food conversion ratio, save feed significantly.As be used to plant soil improvement agents such as organic agronomic crop and top grade flower, also have obvious effect: use this product to increase fertility of land, increase the soil soil fertility, prevent that soil is owing to hardening that chemical fertilizer causes; After probiotic bacterium accounts for the dominant position of soil, can resist the intrusion of assorted bacterium, farm crop are fallen ill less, reduce the use of agricultural chemicals, save medicine and open money, it is residual to reduce medicine; Improve the quality of farm crop, fruit sweetens, and becomes big; Flowers are bright-colored, the florescence is long; Growth stage shortens, and results increase income in advance.The present invention also can be used for environment purification, sewage disposal etc. in addition, promotes fermentation compacting corruption that special efficacy is arranged, but deodorizing, purifies air, and significantly reduces the pollution of ight soil to environment.
Ecological microbial agent method for making of the present invention is simple, utilizes aerobic bacteria bacterium such as milk-acid bacteria anaerobies microorganism mixed yeast and subtilis, the zymotechnique under little aerobic condition, and turning out with the milk-acid bacteria is the complex microbial community of representative.Useful matter that various microorganisms produce in its process of growth and secretory product thereof become separately or the matrix and the raw material of mutually growth, by this symbiosis propagation relation, form a complexity and metastable milk-acid bacteria is the composite ecological microbial agent of representative.
Embodiment:
Below in conjunction with specific embodiment the present invention is described in further detail, but the present invention is not limited to specific embodiment.
Embodiment 1
In fermentor tank, put into pure water and add 3% glucose and 0.03% albumen, be cooled to room temperature after the sterilization in 20 fens of heat 120 degree, press the 1-2% inoculation, little oxygen stir culture 5 days, anaerobic was cultivated 1 day, and PH reduces between the 3.0-4.5, and every milligram contains live lactobacillus bacterium several 1 * 10
5, saccharomycetic viable count is 1 * 10
5, go out jar under the room temperature, be distributed into 500 milliliters of bottles according to service condition, 2 litre flasks, 5 liters of buckets, 10 liters of buckets, 15 liters of buckets, 20 liters of buckets.
Using method:
1, feed agent:
Doubly poultry is supplied with in the back to stoste dilute with water 800-2500, domestic animal is directly drunk, and the volume ratio of different growing stages raw material and water is: breast children phase 1: 2000-2500; Growth stage 1: 1000-1500; Adulthood 1: 800-1000.
Admix in the feed and use, the different growing stages raw material accounts for the feed weight percent and is: breast children phase 0.05-1%; Growth stage 1-3%; Adulthood 3-8%.
To raise chickens is example data statistics such as following table 1, table 2:
Table 2
| Relatively | The check plot | The test site |
| Death toll | 1100 | 949 |
| Forage volume | 1170 | 1068 |
| Mean number is delivered body weight for sale | 5.4 jin | 5.8 jin |
| Feedstuff-meat ratio | 2.0∶1 | 1.8∶1 |
| Medication | The check plot | The test site |
| Nutritional drugs | 750 | 100 yuan |
| Respiratory tract | 300 yuan | 200 yuan |
| Enteron aisle | 4000 yuan | 1500 yuan |
| Vaccine | 400 yuan | 400 yuan |
| Add up to | 5450 yuan | 2200 yuan |
| The ammonia concentration questionnaire | ||
| Ammonia concentration: PPM | The test site | The check plot |
| The 1st week | 100 | 100 |
| The 2nd week | 150 | 550 |
| The 3rd week | 220 | 850 |
| The 4th week | 260 | 1000 |
| The 5th week | 280 | 1400 |
| The 6th week | 300 | 1500 |
2, use as reodorant:
Degree according to stink:, spray colony house with watering can with stoste or with 80-250 times of diluted.
3, environment purification uses:
Sewage disposal time spent graded is handled sewage, and each stoste accounts for water yield volume ratio and is: the 1st time: 1/500 of the water yield; The 2nd time: 1/5000 of the water yield.
4, soil improvement agent and fermentation accelerant:
Fermentation accelerant and plantation organic vegetable usefulness, fruit, the strawberry and the grass usefulness of growing flower, stoste are diluted 250-300 doubly, water to be filled on the farm manure.
Embodiment 2
Prepare live lactobacillus composite ecological microbial agent by embodiment 1 described method, PH reduces between the 3.0-4.5, and every milligram contains live lactobacillus bacterium several 1 * 10
4, milk-acid bacteria comprises lactobacterium casei and plant lactobacillus, the viable count of S. cervisiae is 1 * 10
4
Embodiment 3
Prepare live lactobacillus composite ecological microbial agent by embodiment 1 described method, PH reduces between the 3.0-4.5, and every milligram contains live lactobacillus bacterium several 1 * 10
6, milk-acid bacteria is adopted plant lactobacillus, and yeast bacterium number is 1 * 10
6, yeast comprises S. cervisiae and saccharomyces fragilis, thiobacillus bacterium number is less than 1 * 10
1, Vickers bacterium nitrobacter bacterium number is less than 1 * 10
1
Embodiment 4
Prepare live lactobacillus composite ecological microbial agent by embodiment 1 described method, PH reduces between the 3.0-4.5, and every milligram contains milk-acid bacteria bacterium several 1 * 10
7, milk-acid bacteria comprises Lactobacterium acidophilum and lactobacillus lactis, the bacterium number of viable yeast bacterium is 1 * 10
7, yeast comprises S. cervisiae and Candida arborea, the bacterium number of subtilis, bacillus mesentericus and butyric acid rib bacterium is less than 1 * 10
1Photosynthetic bacterium bacterium number is less than 1 * 10
1
Embodiment 5
Prepare live lactobacillus composite ecological microbial agent by embodiment 1 described method, PH reduces between the 3.0-4.5, and every milligram contains milk-acid bacteria bacterium several 1 * 10
8, milk-acid bacteria comprises lactobacterium casei, plant lactobacillus, Lactobacterium acidophilum, lactobacillus lactis, Streptococcus cremoris, thermophilus streptococcus, Leuconostoc mesenteroides and enterococcus faecalis.Saccharomycetic viable count is 1 * 10
8, yeast comprises yeast saccharomyces cerevisiae, saccharomyces fragilis and Candida arborea, genus bacillus bacterium number is less than 1 * 10
1, comprise subtilis, bacillus mesentericus and butyric acid rib bacterium; Nitrobacteria is adopted the Vickers bacterium nitrobacter, and the bacterium number is less than 1 * 10
1Sulphur bacteria is adopted thiobacillus, and the bacterium number is less than 1 * 10
1Photosynthetic bacterium is a photosynthetic bacterium, and the bacterium number is less than 1 * 10
1
Claims (8)
1, live lactobacillus composite ecological microbial agent, it is characterized in that: it is to be cultivated through ferment tank by the probiotic bacterium that is representative to form with live lactobacillus and yeast, other live probiotics bacterial classifications are selected from nitrobacteria, sulphur bacteria, one or more in photosynthetic bacterium and the genus bacillus.
2, live lactobacillus composite ecological microbial agent according to claim 1 is characterized in that: ecological microbial agent is cultivated through ferment tank by live lactobacillus, yeast, nitrobacteria, sulphur bacteria, photosynthetic bacterium and probiotic bacillus and is formed.
3, live lactobacillus composite ecological microbial agent according to claim 1 and 2 is characterized in that: every milligram of bacterium that contains live lactobacillus of ecological microbial agent is counted 1x10
4-1x10
10, saccharomycetic viable count 1x10
3-1x10
10
4, live lactobacillus composite ecological microbial agent according to claim 1 and 2 is characterized in that: the bacterium number of other live probiotics nitrobacterias, sulphur bacteria, photosynthetic bacterium and genus bacillus is no more than 1x10 respectively
1
5, live lactobacillus composite ecological microbial agent according to claim 1 and 2 is characterized in that: yeast comprises yeast saccharomyces cerevisiae, saccharomyces fragilis and Candida arborea.
6, live lactobacillus composite ecological microbial agent according to claim 1 and 2 is characterized in that: milk-acid bacteria comprises lactobacillus and lactic acid coccus, and lactobacillus comprises lactobacterium casei, plant lactobacillus, Lactobacterium acidophilum and lactobacillus lactis; The lactic acid coccus comprises Streptococcus cremoris, thermophilus streptococcus, Leuconostoc mesenteroides and enterococcus faecalis.
7, live lactobacillus composite ecological microbial agent according to claim 1 and 2 is characterized in that: genus bacillus comprises subtilis, bacillus mesentericus and butyric acid rib bacterium; Nitrobacteria is adopted the Vickers bacterium nitrobacter; Sulphur bacteria is adopted thiobacillus; Photosynthetic bacterium is a photosynthetic bacterium.
8, the method for making of live lactobacillus composite ecological microbial agent according to claim 1 and 2, it is characterized in that: concrete technology: in fermentor tank, put into pure water, add entry gross weight 3-5% glucose and water gross weight 0.01-0.03% albumen, keep the 100-120 degree after the heat, be incubated 15-20 minute, be cooled to room temperature then, press the inoculation of 1-5% concentration, little oxygen stir culture 3-5 days, anaerobic was cultivated 20-24 hour, pH value is reduced between the 3.0-4.5, and every milligram contains the live lactobacillus bacterium and counts 1x10
4-1x10
10, saccharomycetic viable count is 1x10
3-1x10
10, go out jar under the room temperature, promptly make live lactobacillus composite ecological microbial agent by the metering packing.
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|---|---|---|---|
| CNA2009100123316A CN101603016A (en) | 2009-06-30 | 2009-06-30 | Live lactobacillus composite ecological microbial agent |
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|---|---|---|---|
| CNA2009100123316A CN101603016A (en) | 2009-06-30 | 2009-06-30 | Live lactobacillus composite ecological microbial agent |
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Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN101812406B (en) * | 2009-12-30 | 2013-05-08 | 沈阳科丰牧业科技有限公司 | Composite microbial preparation for degrading aflatoxin |
| CN103478443A (en) * | 2013-08-20 | 2014-01-01 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN103725634A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Compound probiotics for planting kiwi fruits |
| CN103725635A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Compound probiotics for strawberry planting |
| CN103756934A (en) * | 2013-12-31 | 2014-04-30 | 陕西万源生物农业科技有限公司 | Compound probiotics for cherry planting |
| CN103766651A (en) * | 2013-12-31 | 2014-05-07 | 陕西万源生物农业科技有限公司 | Compound probiotic for raising chicken |
| CN103789229A (en) * | 2013-12-31 | 2014-05-14 | 陕西万源生物农业科技有限公司 | Composite probiotic for crops and trees planting |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional probiotic |
| CN104370661A (en) * | 2014-10-22 | 2015-02-25 | 杨凌华辕生物产业集团有限公司 | Microecological preparation for grain production safety assurance and preparation method thereof |
| CN104381690A (en) * | 2014-11-25 | 2015-03-04 | 上海创博生态工程有限公司 | Microbial preparation for improving meat quality of Yanbian yellow cattle, and preparation method of microbial preparation |
| CN104522819A (en) * | 2015-01-19 | 2015-04-22 | 中国食品发酵工业研究院 | Pineapple fermented product and preparation method thereof |
| CN105767603A (en) * | 2016-04-06 | 2016-07-20 | 董燕声 | Tortoise farming feed additive |
| CN105918620A (en) * | 2016-05-06 | 2016-09-07 | 江苏东南生物科技有限公司 | Microbial agent for aquatic product feeds |
| CN106107022A (en) * | 2016-07-06 | 2016-11-16 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | A kind of suppression escherichia coli compound microbial preparation used for aquiculture |
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| CN108315276A (en) * | 2018-02-06 | 2018-07-24 | 北京市畜牧总站 | A kind of multifunctional microbial compound deodorizer and its preparation method and application |
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| CN113999803A (en) * | 2021-11-30 | 2022-02-01 | 电子科技大学中山学院 | Deodorizing microbial agent and preparation method thereof |
-
2009
- 2009-06-30 CN CNA2009100123316A patent/CN101603016A/en active Pending
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812406B (en) * | 2009-12-30 | 2013-05-08 | 沈阳科丰牧业科技有限公司 | Composite microbial preparation for degrading aflatoxin |
| CN102283321B (en) * | 2010-06-18 | 2012-08-22 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN102283321A (en) * | 2010-06-18 | 2011-12-21 | 哈尔滨爱特杰牧业有限公司 | Cow rumen fermentation accelerator |
| CN103478443B (en) * | 2013-08-20 | 2014-12-03 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN103478443A (en) * | 2013-08-20 | 2014-01-01 | 沈阳金科丰牧业科技有限公司 | Micro-ecological preparation and application thereof in improvement of production performance, immune performance as well as chicken quality of broiler |
| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| CN103725635A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Compound probiotics for strawberry planting |
| CN103756934A (en) * | 2013-12-31 | 2014-04-30 | 陕西万源生物农业科技有限公司 | Compound probiotics for cherry planting |
| CN103766651A (en) * | 2013-12-31 | 2014-05-07 | 陕西万源生物农业科技有限公司 | Compound probiotic for raising chicken |
| CN103789229A (en) * | 2013-12-31 | 2014-05-14 | 陕西万源生物农业科技有限公司 | Composite probiotic for crops and trees planting |
| CN103725634A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Compound probiotics for planting kiwi fruits |
| CN104152386A (en) * | 2014-08-14 | 2014-11-19 | 河北农业大学 | Multifunctional probiotic |
| CN104152386B (en) * | 2014-08-14 | 2017-12-15 | 河北农业大学 | A kind of multi-functional probiotics |
| CN104370661A (en) * | 2014-10-22 | 2015-02-25 | 杨凌华辕生物产业集团有限公司 | Microecological preparation for grain production safety assurance and preparation method thereof |
| CN104381690A (en) * | 2014-11-25 | 2015-03-04 | 上海创博生态工程有限公司 | Microbial preparation for improving meat quality of Yanbian yellow cattle, and preparation method of microbial preparation |
| CN104522819A (en) * | 2015-01-19 | 2015-04-22 | 中国食品发酵工业研究院 | Pineapple fermented product and preparation method thereof |
| CN104522819B (en) * | 2015-01-19 | 2016-08-03 | 中国食品发酵工业研究院 | A kind of Fructus Ananadis comosi fermented product and preparation method thereof |
| CN105767603A (en) * | 2016-04-06 | 2016-07-20 | 董燕声 | Tortoise farming feed additive |
| CN105918620A (en) * | 2016-05-06 | 2016-09-07 | 江苏东南生物科技有限公司 | Microbial agent for aquatic product feeds |
| CN106107022A (en) * | 2016-07-06 | 2016-11-16 | 苏州市相城区阳澄湖镇东枪塘水产养殖专业合作社 | A kind of suppression escherichia coli compound microbial preparation used for aquiculture |
| CN106431755A (en) * | 2016-09-21 | 2017-02-22 | 深圳富农农业科技有限公司 | Liquid organic biological fertilizer containing black juice of bacteria |
| CN107518009A (en) * | 2017-07-14 | 2017-12-29 | 北京百丰天下生物科技有限公司 | Clear garden bacteria agent applied to germ cleaning and preparation method thereof |
| CN108211692A (en) * | 2017-12-29 | 2018-06-29 | 广州广牧丰生物技术有限公司 | A kind of biological deodorant and its application |
| CN108315276A (en) * | 2018-02-06 | 2018-07-24 | 北京市畜牧总站 | A kind of multifunctional microbial compound deodorizer and its preparation method and application |
| CN111808772A (en) * | 2020-07-20 | 2020-10-23 | 广西农业职业技术学院 | Micro-ecological preparation applied to pelteobagrus fulvidraco breeding and preparation method thereof |
| CN113999803A (en) * | 2021-11-30 | 2022-02-01 | 电子科技大学中山学院 | Deodorizing microbial agent and preparation method thereof |
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Application publication date: 20091216 |