CN104152386A - Multifunctional microecological preparation - Google Patents

Multifunctional microecological preparation Download PDF

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Publication number
CN104152386A
CN104152386A CN201410398072.6A CN201410398072A CN104152386A CN 104152386 A CN104152386 A CN 104152386A CN 201410398072 A CN201410398072 A CN 201410398072A CN 104152386 A CN104152386 A CN 104152386A
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nutrient solution
functional micro
roughage
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CN104152386B (en
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卢海强
谷新晰
谷子林
陈赛娟
刘亚娟
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Heibei Agricultural University
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Heibei Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a multifunctional microecological preparation, belonging to the technical field of preparation of microbial preparations. It comprises the following active substances: lactobacillus casei, Bacillus licheniformis, Bacillus subtilis, Bacillus cereus, Saccharomyces cerevisiae and Pseudomonas stutzeri. The multifunctional microecological composition can be prepared into solution to prepare beneficial bacterium feed, can be used for deodorizing animal excrement and preventing and treating enteritis and diarrhea of raised animals, and has a wide application range.

Description

A kind of multi-functional probiotics
Technical field
The invention belongs to microbial preparation preparing technical field, be specifically related to a kind of multi-functional probiotics, have deodorizing, treatment feeding animals enteritis, cultivates large date fruit and adds lustre to, the multiple efficacies such as increase.
Background technology
At present, Poultry farming has become a very proven technique, but also there is the place of a lot of deficiencies in the management of poultry manure, subject matter is to raise place ight soil to be difficult to timely processing, raise place stink heavier, be unfavorable for the healthy growth of animal, need to take the life that a large amount of antibiosis usually maintain raise poultry, the poultry quality of producing is low, containing Multiple Classes of Antibiotics.
Enteritis and diarrhoea are the modal diseases of feeding animals.Its cause of disease is complicated, and morbidity is anxious, and mortality ratio is high, and feeding animals is produced and formed larger threat.Diarrhoea and enteritis are classified as follows according to its symptom: according to the situation of ight soil, be divided into three kinds, i.e. enteritis, mucoenteritis and diarrhoea; Every generation diarrhoea and the intestinal mucosa person of coming off all classify enteritis as, are divided into serious enteritis and two types of slight enteritis again according to occurring degree, fall ill heavier, and dehydration, appetite reduce or the trencherman that gives up classifies serious enteritis as; Their early stage, obviously dehydration and appetite still can person not classified slight enteritis as; Mucoenteritis is ight soil distortion, with gel-shaped mucus person; There is soft stool and just rare in diarrhoea, there is no gel-shaped mucus and the intestinal mucosa person that do not come off.Common antibiotics or chemicals control in production, although there is certain effect, its drawback is also apparent.Particularly, along with the rise of green waves, green consumption becomes social main flow, and in herding is produced, the use of microbiotic and chemicals is very restricted.Therefore, select its substitute to become one of animal and veterinary scientific and technical personnel long-term and difficult task.
Date is a kind of common cultivating apple trees, and the date that sugariness is larger is especially subject to liking of people, still, date is after maturation, and the epidermis of date contracting wrinkle, even produces segmentation crack, affect the aesthetics of date, after shrinking, the pulp of the inside also tails off, and affects mouthfeel, and date is in process of growth, because its sugariness is large, easily insect infestation, traditional method adopts the method for pesticide spraying immature fruit, causes the residual of agricultural chemicals.
Summary of the invention
The object of the present invention is to provide a kind of multi-functional micro-ecologic assemblage thing, have deodorizing, treatment feeding animals enteritis, cultivates large date fruit and adds lustre to, the multiple efficacies such as increase.
A multi-functional micro-ecologic assemblage thing, the active substance that comprises following component: lactobacterium casei, Bacillus licheniformis, subtilis, bacillus cereus, yeast saccharomyces cerevisiae and Pseudomonas stutzeri.
The active ingredient of described micro-ecologic assemblage thing also comprises Lactobacillus harbinensis.
The nutrient solution of above-mentioned multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 4-6g, beef freezes 1-2g, sucrose 5-10g, lactobacterium casei 5-15x10 8cfu/mL, Bacillus licheniformis 5-15x10 8cfu/mL, subtilis 5-15x10 8cfu/mL, bacillus cereus 5-15x10 8cfu/mL, yeast saccharomyces cerevisiae 5-15x10 8cfu/mL and Pseudomonas stutzeri 5-15x10 8cfu/mL.
The nutrient solution of above-mentioned multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 4-6g, beef freezes 1-2g, sucrose 5-10g, lactobacterium casei 5-15x10 8cfu/mL, Bacillus licheniformis 5-15x10 8cfu/mL, subtilis 5-15x10 8cfu/mL, bacillus cereus 5-15x10 8cfu/mL, yeast saccharomyces cerevisiae 5-15x10 8cfu/mL, Pseudomonas stutzeri 5-15x10 8cfu/mL and Lactobacillus harbinensis 5-15x 10 8cfu/mL.
A deodorizing probiotics, described probiotics is comprised of the material of following weight: the nutrient solution 1-3mL of multi-functional micro-ecologic assemblage thing described in claim 3 or 4, brown sugar 1-2g, lactose 1-2g, calcium carbonate 1-2g, peptone 1-2g, MgSO 40.1-0.2g, water 92-98mL.
Prevent a preparation method for the feed of feeding animals enteritis and diarrhoea, carry out in accordance with the following steps:
(1), by maize straw, beanstalk and peanut seedling are pulverized; According to mass ratio, be mixed into mixed roughage at 1: 1: 1;
(2) to spraying and account for the brown sugar that its mass percent is 0.5-1% in mixed roughage, or add the Semen Maydis powder that accounts for its mass percent 4-8%, make and add roughage;
(3) to adding in roughage described in step (2), add the water that accounts for its mass percent 50-55%, stir, make and add water feed;
(4) to adding in water feed, spray and account for the nutrient solution that its mass ratio is described multi-functional micro-ecologic assemblage thing of 0.1-1%;
(5) anaerobically fermenting 7-30 days, dries standby.
Above-mentioned multi-functional micro-ecologic assemblage thing is in deodorizing, and date goes wrinkle to add lustre to and prevents and treat the application in feeding animals enteritis and diarrhoea.
Beneficial effect of the present invention: multi-functional micro-ecologic assemblage thing of the present invention can wiring solution-forming, prepares probiotics feed, can be used for the deodorizing of animal excrement, prevention and treatment feeding animals enteritis and diarrhoea, applied range.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
A nutrient solution for multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 5g, beef freezes 1.5g, sucrose 8g, lactobacterium casei 10x10 8cfu/mL, Bacillus licheniformis 8x10 8cfu/mL, subtilis 11x10 8cfu/mL, bacillus cereus 10x10 8cfu/mL, yeast saccharomyces cerevisiae 8x10 8cfu/mL and Pseudomonas stutzeri 10x10 8cfu/mL.
Get the nutrient solution 1mL of above-mentioned multi-functional micro-ecologic assemblage thing, brown sugar 1g, lactose 1g, calcium carbonate 1g, peptone 1g, MgSO 40.1g, adds water 92mL, mixes, and makes deodorizing solution.
Get above-mentioned deodorizing solution 100mL, spray and be once sprayed on 100m week about 2hen house ground, the ight soil stink basically eliminate in hen house.
Embodiment 2
A nutrient solution for multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 6g, beef freezes 2g, sucrose 10g, lactobacterium casei 15x10 8cfu/mL, Bacillus licheniformis 5x10 8cfu/mL, subtilis 5x10 8cfu/mL, bacillus cereus 15x10 8cfu/mL, yeast saccharomyces cerevisiae 5x10 8cfu/mL and Pseudomonas stutzeri 15x10 8cfu/mL.
Get the nutrient solution 3mL of above-mentioned multi-functional micro-ecologic assemblage thing, brown sugar 2g, lactose 2g, calcium carbonate 2g, peptone 2g, MgSO 40.2g, adds water 98mL, mixes, and makes deodorizing solution.
Get above-mentioned deodorizing solution 100mL, spray and be once sprayed on 100m week about 2hen house ground, the ight soil stink basically eliminate in hen house.
Embodiment 3
A nutrient solution for multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 5g, beef freezes 2g, sucrose 8g, lactobacterium casei 10x10 8cfu/mL, Bacillus licheniformis 10x10 8cfu/mL, subtilis 10x10 8cfu/mL, bacillus cereus 10x10 8cfu/mL, yeast saccharomyces cerevisiae 10x10 8cfu/mL, Pseudomonas stutzeri 10x10 8cfu/mL and Lactobacillus harbinensis 10x 10 8cfu/mL.
Get the nutrient solution 2mL of above-mentioned multi-functional micro-ecologic assemblage thing, brown sugar 1.5g, lactose 1.5g, calcium carbonate 1.5g, peptone 1.5g, MgSO 40.15g, adds water 96mL, mixes, and makes deodorizing solution.
Get above-mentioned nutrient solution 100mL, spray and be once sprayed on 100m week about 2hen house ground, the ight soil stink basically eliminate in hen house.
Embodiment 4
A nutrient solution for multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 6g, beef freezes 1g, sucrose 5g, lactobacterium casei 5x10 8cfu/mL, Bacillus licheniformis 15x10 8cfu/mL, subtilis 15x10 8cfu/mL, bacillus cereus 15x10 8cfu/mL, yeast saccharomyces cerevisiae 15x10 8cfu/mL and Pseudomonas stutzeri 15x10 8cfu/mL.
Adopt the method for the feed of above-mentioned nutrient solution preparation prevention feeding animals enteritis and diarrhoea, carry out in accordance with the following steps:
(1), by maize straw, beanstalk and peanut seedling are pulverized; According to mass ratio, be mixed into mixed roughage at 1: 1: 1;
(2) to spraying in mixed roughage, to account for its mass percent be 1% brown sugar, or add the Semen Maydis powder that accounts for its mass percent 5%, makes and add roughage;
(3) to adding in roughage described in step (2), add the water that accounts for its mass percent 52%, stir, make and add water feed;
(4) to adding in water feed to spray, account for the nutrient solution that its mass ratio is described multi-functional micro-ecologic assemblage thing of 0.5%;
(5) anaerobically fermenting 7-30 days, dries standby.
After drying, according to 3%, 5%, 7% biologic coarse fodder of daily ration (test I, II, III group), substitute respectively the equivalent wheat bran in basal diet, control group fed basal diet.
Result shows (table 1): the roughage of fermentation can substitute wheat bran and feed rabbit, has improved food consumption, the speed of growth 21.74%, has reduced feed intake 16.33%.
The impact of table 1 fermentation roughage on Rex Rabbit production performance
Group Starting weight (g/ only) End heavy (g/ only) Day weight gain (g/ only) Daily material consumption (g/ only) Feed consumption weightening finish ratio
Examination I 614.60±27.12 1483.16±31. 31.02±0.51 Aa 93.54±0.14 Aa 3.02±0.51 a
Examination II 610.00±29.58 1423.96±37. 29.07±0.76 Ab 91.25±1.81 ABab 3.14±0.42 a
Examination III 613.15±15.27 1471.91±14. 30.67±0.40 Aa 92.15±1.03 ABa 3.00±0.21 a
Control group 610.94±30.26 1324.38±41. 25.48±0.08 Bc 88.99±1.51 Bb 3.49±1.02 a
The fermentation High-Forage Diets of feeding can significantly reduce diarrhea rate (table 2), and in the scope of addition 3~7%, along with the increase of addition, more remarkable effect.
The impact of the fermentation roughage of table 2 different levels on Rex Rabbit diarrhea rate
Group Examination I Examination II Examination III Control group
Diarrhea frequency (%) 0.54 0.36 0.19 2.14
In daily ration, add fermentation roughage and can significantly improve feed digestibility: 2~3 percentage points of crude protein 5~7, robust fibre 4~5, crude fat.
The impact of table 3 fermentation roughage on Rex Rabbit nutrient digestion rate
Group Crude protein (%) Robust fibre (%) Crude fat (%)
Examination I 75.87±0.73 A 23.95±1.87 a 76.93±0.84 ab
Examination II 75.32±1.09 A 24.64±1.09 a 77.72±1.36 ab
Examination III 77.04±0.64 A 25.01±1.21 a 78.80±1.06 a
Control group 70.76±1.21 B 20.07±2.43 b 75.01±2.12 b
Embodiment 5
A nutrient solution for multi-functional micro-ecologic assemblage thing, described nutrient solution consists of following component: deionized water 1000mL, yeast extract paste 6g, beef freezes 2g, sucrose 10g, lactobacterium casei 15x10 8cfu/mL, Bacillus licheniformis 15x10 8cfu/mL, subtilis 15x10 8cfu/mL, bacillus cereus 15x10 8cfu/mL, yeast saccharomyces cerevisiae 15x10 8cfu/mL, Pseudomonas stutzeri 15x10 8cfu/mL and Lactobacillus harbinensis 5-15x10 8cfu/mL.
Adopt the method for the feed of above-mentioned nutrient solution preparation prevention feeding animals enteritis and diarrhoea, carry out in accordance with the following steps:
(1), by maize straw, beanstalk and peanut seedling are pulverized; According to mass ratio, be mixed into mixed roughage at 1: 1: 1;
(2) to spraying in mixed roughage, to account for its mass percent be 0.6% brown sugar, or add the Semen Maydis powder that accounts for its mass percent 8%, makes and add roughage;
(3) to adding in roughage described in step (2), add the water that accounts for its mass percent 53%, stir, make and add water feed;
(4) to adding in water feed to spray, account for the nutrient solution that its mass ratio is described multi-functional micro-ecologic assemblage thing of 0.6%;
(5) anaerobically fermenting 7-30 days, dries standby.
After drying, according to 3%, 5%, 7% biologic coarse fodder of daily ration (test I, II, III group), substitute respectively the equivalent wheat bran in basal diet, control group fed basal diet.
Result shows (table 4): the roughage of fermentation can substitute wheat bran and feed rabbit, has improved food consumption, the speed of growth 32.74%, has reduced feed intake 27.33%.
The impact of table 4 fermentation roughage on Rex Rabbit production performance
Group Starting weight (g/ only) End heavy (g/ only) Day weight gain (g/ only) Daily material consumption (g/ only) Feed consumption weightening finish ratio
Examination I 614.60±27.12 1783.16±31. 41.02±0.51 Aa 83.54±0.14 Aa 3.02±0.51 a
Examination II 610.00±29.58 1723.96±37. 40.07±0.76 Ab 81.25±1.81 ABab 3.14±0.42 a
Examination III 613.15±15.27 1771.91±14. 39.67±0.40 Aa 82.15±1.03 ABa 3.00±0.21 a
Control group 610.94±30.26 1324.38±41. 25.48±0.08 Bc 88.99±1.51 Bb 3.49±1.02 a
The fermentation High-Forage Diets of feeding can significantly reduce diarrhea rate (table 5), and in the scope of addition 3~7%, along with the increase of addition, more remarkable effect.
The impact of the fermentation roughage of table 5 different levels on Rex Rabbit diarrhea rate
Group Examination I Examination II Examination III Control group
Diarrhea frequency (%) 0.44 0.33 0.12 2.14
In daily ration, add fermentation roughage and can significantly improve feed digestibility: 2~3 percentage points of crude protein 5~7, robust fibre 4~5, crude fat.
The impact of table 6 fermentation roughage on Rex Rabbit nutrient digestion rate
Group Crude protein (%) Robust fibre (%) Crude fat (%)
Examination I 75.88±0.73 A 22.95±1.87 a 76.93±0.84 ab
Examination II 74.32±1.09 A 22.64±1.09 a 77.72±1.36 ab
Examination III 76.04±0.64 A 24.01±1.21 a 78.80±1.06 a
Control group 71.76±1.21 B 21.07±2.43 b 75.01±2.12 b

Claims (7)

1. a multi-functional micro-ecologic assemblage thing, is characterized in that the active substance that comprises following component: lactobacterium casei, Bacillus licheniformis, subtilis, bacillus cereus, yeast saccharomyces cerevisiae and Pseudomonas stutzeri.
2. a kind of multi-functional micro-ecologic assemblage thing according to claim 1, is characterized in that, the active ingredient of described micro-ecologic assemblage thing also comprises Lactobacillus harbinensis.
3. the nutrient solution of multi-functional micro-ecologic assemblage thing described in claim 1, is characterized in that, described nutrient solution consists of following component: deionized water 1000mL, and yeast extract paste 4-6g, beef freezes 1-2g, sucrose 5-10g, lactobacterium casei 5-15x10 8cfu/mL, Bacillus licheniformis 5-15x10 8cfu/mL, subtilis 5-15x10 8cfu/mL, bacillus cereus 5-15x10 8cfu/mL, yeast saccharomyces cerevisiae 5-15x10 8cfu/mL and Pseudomonas stutzeri 5-15x10 8cfu/mL.
4. the nutrient solution of multi-functional micro-ecologic assemblage thing described in claim 2, is characterized in that, described nutrient solution consists of following component: deionized water 1000mL, and yeast extract paste 4-6g, beef freezes 1-2g, sucrose 5-10g, lactobacterium casei 5-15x10 8cfu/mL, Bacillus licheniformis 5-15x10 8cfu/mL, subtilis 5-15x10 8cfu/mL, bacillus cereus 5-15x10 8cfu/mL, yeast saccharomyces cerevisiae 5-15x10 8cfu/mL, Pseudomonas stutzeri 5-15x10 8cfu/mL and Lactobacillus harbinensis 5-15x10 8cfu/mL.
5. a deodorizing probiotics, is characterized in that, described probiotics is comprised of the material of following weight: the nutrient solution 1-3mL of multi-functional micro-ecologic assemblage thing described in claim 3 or 4, brown sugar 1-2g, lactose 1-2g, calcium carbonate 1-2g, peptone 1-2g, MgSO 40.1-0.2g, water 92-98mL.
6. a preparation method who prevents the feed of feeding animals enteritis and diarrhoea, is characterized in that, carries out in accordance with the following steps:
(1), by maize straw, beanstalk and peanut seedling are pulverized; According to mass ratio, be mixed into mixed roughage at 1: 1: 1;
(2) to spraying and account for the brown sugar that its mass percent is 0.5-1% in mixed roughage, or add the Semen Maydis powder that accounts for its mass percent 4-8%, make and add roughage;
(3) to adding in roughage described in step (2), add the water that accounts for its mass percent 50-55%, stir, make and add water feed;
(4) to adding in water feed, spray the nutrient solution that accounts for multi-functional micro-ecologic assemblage thing described in claim 3 that its mass ratio is 0.1-1% or claim 4;
(5) anaerobically fermenting 7-30 days, dries standby.
7. described in claim 1 or 2, multi-functional micro-ecologic assemblage thing is in deodorizing, and date goes wrinkle to add lustre to and prevents and treat the application in feeding animals enteritis and diarrhoea.
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104322894A (en) * 2014-11-28 2015-02-04 蒋小春 Preparation method of microbial preparation for feed
CN104397357A (en) * 2014-12-11 2015-03-11 河北农业大学 Premix for improving reproductive performance of female rabbits
CN104686838A (en) * 2015-02-11 2015-06-10 青岛根源生物技术集团有限公司 Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive
CN104814355A (en) * 2015-05-23 2015-08-05 昆明饲宝饲料有限责任公司 Deodorant traditional Chinese medicine feed additive for chicken, preparation method and application
CN106720942A (en) * 2016-12-15 2017-05-31 上海创博生态工程有限公司 A kind of additive for microbe feedstuff for improving Eriocheir sinensis meat and preparation method thereof
CN108823114A (en) * 2018-05-02 2018-11-16 中国石油大学(华东) A kind of cultural method of rubbish deodorization biological enzyme and its application
CN109430521A (en) * 2018-12-29 2019-03-08 上海邦成生物工程有限公司 A kind of compound feeding micro-ecological preparation and preparation method thereof
CN110699299A (en) * 2019-11-14 2020-01-17 乌拉特前旗荣生大地生物科技饲料有限责任公司 Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof
CN113278560A (en) * 2021-06-15 2021-08-20 无锡拜弗德生物科技有限公司 Microecological preparation for improving pet odor

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104322894A (en) * 2014-11-28 2015-02-04 蒋小春 Preparation method of microbial preparation for feed
CN104397357A (en) * 2014-12-11 2015-03-11 河北农业大学 Premix for improving reproductive performance of female rabbits
CN104686838A (en) * 2015-02-11 2015-06-10 青岛根源生物技术集团有限公司 Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive
CN104814355A (en) * 2015-05-23 2015-08-05 昆明饲宝饲料有限责任公司 Deodorant traditional Chinese medicine feed additive for chicken, preparation method and application
CN106720942A (en) * 2016-12-15 2017-05-31 上海创博生态工程有限公司 A kind of additive for microbe feedstuff for improving Eriocheir sinensis meat and preparation method thereof
CN108823114A (en) * 2018-05-02 2018-11-16 中国石油大学(华东) A kind of cultural method of rubbish deodorization biological enzyme and its application
CN109430521A (en) * 2018-12-29 2019-03-08 上海邦成生物工程有限公司 A kind of compound feeding micro-ecological preparation and preparation method thereof
CN110699299A (en) * 2019-11-14 2020-01-17 乌拉特前旗荣生大地生物科技饲料有限责任公司 Bacillus licheniformis X173 strain for producing urease inhibitor and application thereof
CN113278560A (en) * 2021-06-15 2021-08-20 无锡拜弗德生物科技有限公司 Microecological preparation for improving pet odor

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