CN108823114A - A kind of cultural method of rubbish deodorization biological enzyme and its application - Google Patents
A kind of cultural method of rubbish deodorization biological enzyme and its application Download PDFInfo
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- CN108823114A CN108823114A CN201810408029.1A CN201810408029A CN108823114A CN 108823114 A CN108823114 A CN 108823114A CN 201810408029 A CN201810408029 A CN 201810408029A CN 108823114 A CN108823114 A CN 108823114A
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- biological enzyme
- rubbish
- bacillus subtilis
- cultural method
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
Abstract
The invention discloses a kind of cultural method of rubbish deodorization biological enzyme and its applications, belong to environment protection field.It is according to the raw material of mass parts：2 parts of bacillus subtilis, 2 parts of bacillus licheniformis, 2 parts of Pseudomonas stutzeri, 300 parts of fermentation medium, cultural method the specific steps are：(A) landfill leachate is dissolved into oscillation layering, takes lower layer's fermentation liquid culture, it is repeated multiple times；(B) fermentation liquid in gradient dilution step (A) is cultivated, domestication, until obtaining two plants of pure bacterium, i.e. bacillus subtilis and bacillus licheniformis；(C) pure bacterium obtained in step (B) and Pseudomonas stutzeri are pressed 1:1:1 is mixed into flora；(D) microflora fermentation is obtained into rubbish deodorization biological enzyme.Present invention process process is simple, and object generation without secondary pollution, has the characteristics that wide spectrum, efficient, environmentally friendly, nontoxic, noresidue in incubation.It can be used for house refuse deodorization and the control of public lavatory peculiar smell, environmental sound after use.
Description
Technical field
The present invention relates to environment protection field, the cultural method of specially a kind of rubbish deodorization biological enzyme and its application.
Background technique
With the rapid development of society the quantity of house refuse is increasing, lead to atmosphere, soil, surface water and underground water
By serious pollution, and the organic principle ratio contained in rubbish is high, complicated component, and including having pathogenic disease
Pathogenic microorganism, helminth and detection device for multi metallic elements are stacked for a long time in addition, and various unpleasant foul gas can be through micro- life
Object releases the gases such as ammonia, hydrogen sulfide, mercaptan, thioether, formaldehyde, acetaldehyde and volatile fatty acid after decomposing organic principle.It should
A possibility that gas not only causes a large amount of mosquito, fly, leads to transmission increase brings greatly human health life
Harm, and the gas highly volatile generated escapes into and causes environmental pollution in atmosphere, influences city appearance, or even hinder ecology
Civilization construction, it can be seen that the malodor problem of house refuse is urgently to be resolved.
The processing common method of rubbish stench has absorption method, combustion method, essence masking method and energetic ion both at home and abroad at present
Method.Absorption method includes chemical agent and active carbon adsorption, the former needs to consume a large amount of chemical agents, the latter Ze Yi blocking and more
It changes frequently, maintenance cost is higher；Combustion method burns odorant at high temperature, is decomposed into the inorganic matters such as water and carbon dioxide,
But it is suitable for the organic exhaust gas of high concentration, high heating value, it is seldom applied in general deodorization engineering；Essence covers method using spray
Compound essence is spilt to cover stench, it is palliative, and a variety of organic matters can be added to people during essence synthesis
Body has certain injury；In addition, carrying out garbage disposal using energetic ion method, the ozone and ion generated easily causes environment
Pollution and corrosion equipment, or even injury human health.
Undoubtedly presently the most environmentally friendly method is to carry out rubbish deodorization using microorganism, by microorganism and beneficial mushroom come
Organic matter is decomposed, the generation and breeding for preventing the stinks such as organic matter generation ammonia, hydrogen sulfide and reduction harmful bacteria are only radical cure
Originally.Therefore, a kind of deodorizing effect of invention is good, to human body, environmentally friendly environment friendly biological enzyme, meets instantly environmentally friendly
Demand, the garbage disposal to public places such as application of city life garbage field, public lavatory, food city dustbins are an effective sides
Method.
Summary of the invention
The first object of the present invention is the problem obvious for current house refuse stench, and it is raw to provide a kind of rubbish deodorization
Object enzyme inhibits bacterium, fungi in rubbish, contains its growth and breeding, kill mosquitos and flies, bedbug and the flea generated in rubbish, shows
Write reduce or the stink that removes rubbish, in addition, the deodorization biological enzyme in the present invention also have wide spectrum, it is efficient, environmentally friendly, nontoxic, without residual
The characteristics of staying.
The second object of the present invention is to provide a kind of cultural method of rubbish deodorization biological enzyme, and this method is easy to operate,
Have the characteristics that taste removal is thorough, quick using the deodorization biological enzyme of this method culture.
The third object of the present invention is to provide a kind of application of rubbish deodorization biological enzyme, makes above-mentioned deodorization biological enzyme can be fast
Speed is effectively removed house refuse stench and the control of public lavatory peculiar smell.
In order to achieve the above objectives, the technical solution adopted by the present invention is as follows：
A kind of rubbish deodorization biological enzyme is according to the raw material of mass parts：Bacillus subtilis (Bacillus subtilis)
2 parts, 2 parts of bacillus licheniformis (Bacillus licheniformis), Pseudomonas stutzeri (Pseudomonas
Stutzeri) 2 parts；300 parts of fermentation medium, cultural method, the specific steps are：
(A) landfill leachate is utilized into physiological saline solution, bacteria suspension is accessed inorganic salts by stratification after shaking table oscillation
It is cultivated in fluid nutrient medium, the fermentation liquid for taking lower layer to contain thallus, which is forwarded in inorganic salt liquid culture medium, to be continued to cultivate, so
It is repeated a number of times；；
(B) it draws quantification steps (A) to continue to cultivate the fermentation liquid in gained rubbish inorganic salt liquid culture medium, carries out gradient
Dilution, is applied on solid medium and cultivates several days, tame repeatedly, until obtain two plants of pure bacterium, i.e., bacillus subtilis and
Bacillus licheniformis；
(C) step (B) isolated bacillus subtilis and bacillus licheniformis and China Microbiological bacterium will be preserved in
The Pseudomonas stutzeri of kind preservation administration committee common micro-organisms center, three kinds of bacterium are mixed into flora in proportion；
(D) the fermented culture medium culture of flora in step (C) is obtained into rubbish deodorization biological enzyme.
Preferably, telling fermentation liquid in step (A) is that landfill leachate is placed on shaking table oscillation with physiological saline solution
Afterwards, stratification, by bacteria suspension access minimal medium in, 10-15 DEG C shaking table culture 10-15 days, take lower layer to contain bacterium
The fermentation liquid of body, which is forwarded in minimal medium, continues culture 10-15 days, carries out 3-6 times repeatedly.
More optimized, two plants of pure bacterium in the step (B), i.e. bacillus subtilis and bacillus licheniformis are by fermentation liquid
It carries out gradient dilution to be seeded on solid medium, is cultivated 8-15 days at 28-33 DEG C, the bacterium colony of culture is observed, select
The solid medium of moderate number is selected, scribing line culture is carried out, is repeated, until obtaining two plants of pure bacterium, i.e. bacillus subtilis
And bacillus licheniformis.
It is further preferred that flora is that bacillus subtilis, bacillus licheniformis and Amur is false single in the step (C)
Born of the same parents bacterium presses 1:1:1 ratio mixing.
Further optimize, fermentation medium is by mass parts raw material in the step (D)：2.5 parts of hydrolysis sugar, corn
2.5 parts of slurry, 1 part of vinasse, 0.5 part of urea, 93.5 parts of pure water are 6.8-7.0 in pH, cultivate and obtain at 30 DEG C.
Beneficial effects of the present invention are：
Present invention process process is simple, generates in the production process without secondary pollution, pure green environmental protection.It can be used for giving birth to
Rubbish deodorization living and the control of public lavatory peculiar smell, environmental sound after use.Deodorization biological enzyme in the present invention can inhibit rubbish
Bacterium, fungi in rubbish contain its growth and breeding, reduce mosquitos and flies, bedbug and the flea generated in rubbish, significantly reduce or remove
Garbage odor, in addition, this deodorant also has the characteristics that wide spectrum, efficient, environmentally friendly, nontoxic, noresidue.
Specific embodiment
Specific embodiments of the present invention will be described in further detail with reference to embodiments.
Embodiment 1
A kind of rubbish deodorization biological enzyme is according to the raw material of mass parts：Bacillus subtilis (Bacillus subtilis)
2 parts, 2 parts of bacillus licheniformis (Bacillus licheniformis), Pseudomonas stutzeri (Pseudomonas
Stutzeri) 2 parts；Its cultural method, 300 parts of fermentation medium, the specific steps are：
(A) landfill leachate is utilized into physiological saline solution, bacteria suspension is accessed inorganic salts by stratification after shaking table oscillation
It is cultivated in fluid nutrient medium, the fermentation liquid for taking lower layer to contain thallus, which is forwarded in inorganic salt liquid culture medium, to be continued to cultivate, so
It is repeated a number of times；
(B) it draws quantification steps (A) to continue to cultivate the fermentation liquid in gained rubbish inorganic salt liquid culture medium, carries out gradient
Dilution, is applied on solid medium and cultivates several days, tame repeatedly, until obtain two plants of pure bacterium, i.e., bacillus subtilis and
Bacillus licheniformis；
(C) step (B) isolated bacillus subtilis and bacillus licheniformis and China Microbiological bacterium will be preserved in
The Pseudomonas stutzeri of kind preservation administration committee common micro-organisms center, three kinds of bacterium are mixed into flora in proportion；
(D) fermented culture medium culture obtains rubbish deodorization biological enzyme.
Embodiment 2
A kind of rubbish deodorization biological enzyme is according to the raw material of mass parts：Bacillus subtilis (Bacillus subtilis)
1.5 parts, 1.5 parts of bacillus licheniformis (Bacillus licheniformis), Pseudomonas stutzeri (Pseudomonas
Stutzeri) 1.5 parts；Its cultural method, 350 parts of fermentation medium, the specific steps are：
(A) landfill leachate is utilized into physiological saline solution, bacteria suspension is accessed inorganic salts by stratification after shaking table oscillation
It is cultivated in fluid nutrient medium, the fermentation liquid for taking lower layer to contain thallus, which is forwarded in inorganic salt liquid culture medium, to be continued to cultivate, so
It is repeated a number of times；
(B) it draws quantification steps (A) to continue to cultivate the fermentation liquid in gained rubbish inorganic salt liquid culture medium, carries out gradient
Dilution, is applied on solid medium and cultivates several days, tame repeatedly, until obtain two plants of pure bacterium, i.e., bacillus subtilis and
Bacillus licheniformis；
(C) step (B) isolated bacillus subtilis and bacillus licheniformis and China Microbiological bacterium will be preserved in
The Pseudomonas stutzeri of kind preservation administration committee common micro-organisms center, three kinds of bacterium are mixed into flora in proportion；
(D) fermented culture medium culture obtains rubbish deodorization biological enzyme.
Embodiment 3
A kind of rubbish deodorization biological enzyme is according to the raw material of mass parts：Bacillus subtilis (Bacillus subtilis)
1 part, 1 part of bacillus licheniformis (Bacillus licheniformis), Pseudomonas stutzeri (Pseudomonas
Stutzeri) 1 part；Its cultural method, 400 parts of fermentation medium, the specific steps are：
(A) landfill leachate is utilized into physiological saline solution, bacteria suspension is accessed inorganic salts by stratification after shaking table oscillation
It is cultivated in fluid nutrient medium, the fermentation liquid for taking lower layer to contain thallus, which is forwarded in inorganic salt liquid culture medium, to be continued to cultivate, so
It is repeated a number of times；
(B) it draws quantification steps (A) to continue to cultivate the fermentation liquid in gained rubbish inorganic salt liquid culture medium, carries out gradient
Dilution, is applied on solid medium and cultivates several days, tame repeatedly, until obtain two plants of pure bacterium, i.e., bacillus subtilis and
Bacillus licheniformis；
(C) step (B) isolated bacillus subtilis and bacillus licheniformis and China Microbiological bacterium will be preserved in
The Pseudomonas stutzeri of kind preservation administration committee common micro-organisms center, three kinds of bacterium are mixed into flora in proportion；
(D) fermented culture medium culture obtains rubbish deodorization biological enzyme.
To indicate that the effect of deodorization biological enzyme in the present invention carries out processing effect using certain city's Biohazard Waste Disposal as research object
Fruit experimental verification.
The deodorization biological enzyme of example 1-3 is sprayed in rubbish surface and landfill leachate, the reaction time is 3 hours, inspection
Survey the content of hydrogen sulfide and ammonia.
H before and after 1 Biohazard Waste Disposal rubbish of table and landfill leachate sprinkling deodorization biological enzyme2The content balance data of S.
| Hydrogen sulfide (mg/m3) | Ammonia (mg/m3) | |
| Residential block air standard | 0.01 | 0.2 |
| Before spraying biological enzyme | 1.478 | 2.198 |
| Example 1 | 0.00749 | 0.175 |
| Example 2 | 0.00821 | 0.186 |
| Example 3 | 0.00983 | 0.199 |
As it can be seen from table 1 the hydrogen sulfide and ammonia removal rate of Biohazard Waste Disposal are up to 90% after sprinkling deodorization biological enzyme
More than, basically reach the requirement of residential block air standard.So this product processing after not only can effectively remove solid refuse and
The stench of landfill leachate, and have disinfection concurrently, the practicality is stronger.
Claims (7)
1. a kind of rubbish deodorization biological enzyme, described to be characterized in that, it is according to the raw material of mass parts：Bacillus subtilis
2 parts of (Bacillus subtilis), 2 parts of bacillus licheniformis (Bacillus licheniformis), Pseudomonas stutzeri
2 parts, 300 parts of fermentation medium of (Pseudomonas stutzeri).
2. a kind of cultural method of rubbish deodorization biological enzyme described in claim 1, which is characterized in that include the following steps：
(A) landfill leachate is utilized into physiological saline solution, bacteria suspension is accessed inorganic salts culture by stratification after shaking table oscillation
It is cultivated in base, the fermentation liquid for taking lower layer to contain thallus, which is forwarded to, to be continued to cultivate in minimal medium, is carried out repeatedly multiple；
(B) it draws quantification steps (A) to continue to cultivate the fermentation liquid in gained rubbish minimal medium, carries out gradient dilution, apply
Several days are cultivated on cloth to solid medium, are tamed repeatedly, until obtaining two plants of pure bacterium, i.e. bacillus subtilis and lichens gemma
Bacillus；
(C) step (B) isolated bacillus subtilis and bacillus licheniformis are protected with Chinese microorganism strain is preserved in
The Pseudomonas stutzeri of administration committee's common micro-organisms center is hidden, three kinds of bacterium are mixed into flora in proportion；
(D) the fermented culture medium culture of flora in step (C) is obtained into rubbish deodorization biological enzyme.
3. a kind of cultural method of rubbish deodorization biological enzyme according to claim 2, which is characterized in that the step (A)
Fermentation liquid is after landfill leachate is placed on shaking table oscillation with physiological saline solution, and stratification accesses bacteria suspension inorganic
In salt culture medium, 10-15 DEG C shaking table culture 10-15 days, the fermentation liquid for taking lower layer to contain thallus is forwarded to minimal medium
In continue culture 10-15 days, repeatedly progress 3-6 times.
4. a kind of cultural method of rubbish deodorization biological enzyme according to claim 2, which is characterized in that the step (B)
In two plants of pure bacterium, i.e., bacillus subtilis and bacillus licheniformis be by fermentation liquid progress gradient dilution be seeded in solid medium
On, it is cultivated 8-15 days at 28-33 DEG C, the bacterium colony of culture is observed, selected the solid medium of moderate number, drawn
Line culture, is repeated, until obtaining two plants of pure bacterium, i.e. bacillus subtilis and bacillus licheniformis.
5. a kind of cultural method of rubbish deodorization biological enzyme according to claim 2, which is characterized in that the step (B)
Middle solid medium is become by quality group：0.5 part of beef extract, 1 part of peptone, 0.5 part of NaCl, 98 parts of pure water are 7.0- in pH
7.2,121 DEG C of steam sterilizing 20min.
6. a kind of cultural method of rubbish deodorization biological enzyme according to claim 2, which is characterized in that the step (C)
Middle flora is by bacillus subtilis, bacillus licheniformis and Pseudomonas stutzeri by 1:1:1 ratio mixing.
7. a kind of cultural method of rubbish deodorization biological enzyme according to claim 2, which is characterized in that the step (D)
Middle fermentation medium is by mass parts raw material：2.5 parts of hydrolysis sugar, 2.5 parts of corn pulp, 1 part of vinasse, 0.5 part of urea, pure water 93.5
Part is 6.8-7.0 in pH, cultivates and obtains at 30 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113337439A (en) * | 2021-06-18 | 2021-09-03 | 北京天益源生物科技有限公司 | Quick-acting deodorant and preparation method thereof |
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Application publication date: 20181116 |