CN107058153A - A kind of biological deodorizing composite bacteria agent and its preparation method and application - Google Patents
A kind of biological deodorizing composite bacteria agent and its preparation method and application Download PDFInfo
- Publication number
- CN107058153A CN107058153A CN201611130257.4A CN201611130257A CN107058153A CN 107058153 A CN107058153 A CN 107058153A CN 201611130257 A CN201611130257 A CN 201611130257A CN 107058153 A CN107058153 A CN 107058153A
- Authority
- CN
- China
- Prior art keywords
- composite bacteria
- bacteria agent
- parts
- microbial inoculum
- biological deodorizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to biological deodorizing technical field, specifically related to a kind of biological deodorizing composite bacteria agent and its preparation and application, 50 100 parts of pseudomonas microbial inoculum (Pseudomonas), 25 80 parts of bacillus microbial inoculum (Bacillus), the Trichoderma viride that the present invention includes calculating in parts by mass belong to 20 50 parts of microbial inoculum (Trichoderma viride), 20 30 parts of streptomyces microbial inoculum (Streptomyces)；Peculiar smell removes and all achieves good result in the manure pit deodorization that the biological deodorizing composite bacteria agent of the present invention is given up for livestock and kitchen garbage；Not only wide adaptability, toxigenic capacity are low, easy to use but also deodorizing effect is good.
Description
Technical field
The invention belongs to biological deodorizing technical field, and in particular to a kind of biological deodorizing composite bacteria agent and preparation method thereof and
Using.
Background technology
Foul smell is one of big public hazards in the world seven, often results in bacteria breed, and virus procreation for a long time, is passed through and administers foul smell
Method be manually to bury lid nothing but, or sprayed with chemicals smelly (such as injection air freshener) hidden with perfume, can neither thoroughly removed
It is smelly, again can the secondary pollution caused by remaining medicine poison.In Traditional compost, biogas anaerobic fermentation, kitchen garbage fermentation, refuse landfill
Etc. each process field, foul smell is always to influence the direct and matter of utmost importance of people's acceptance level.During as hydrogen sulfide, amine,
Ammonia isoconcentration is too high not only to produce pungent stink, more harm can be produced to the body of people, while deriving serious air
And environmental pollution.In organic matter-water-organic matter compost or fermentation process, microorganism conversion produces substantial amounts of Ammonia material using organic matter,
A part of Ammonia is nitrate nitrogen or synthesis Self substances by microorganism conversion, and a part of then then generation of volatilizing in a gaseous form is pungent
Property smell, while will also result in the loss of nitrogen in compost or fermentation process, labile organic compound decomposes release ammonia in the environment
Gas is the topmost reason to form foul smell.
With social progress, economic development, the enhancing that people's environment is known and the continuous improvement of quality of life, foul gas control
System has increasingly been paid attention to process problem.Therefore, it is necessary to practical ways are taken, the effluvium that these regions are produced
Body carries out purified treatment, improves the environmental quality on its space and periphery.
The method of conventional processing foul smell has absorption method, combustion method, energetic ion method and bioanalysis both at home and abroad at present.Absorption
Method includes soda acid liquid medicine and washed and charcoal absorption, and the former is needed to consume a large amount of chemical agents, and the latter Ze Yi is blocked, and changes frequency
Numerous, maintenance cost is higher；Combustion method is suitable for high concentration, the organic exhaust gas of high heating value, seldom should in general deodorization engineering
With；The ozone that energetic ion method is produced directly is discharged, it is possible to can endanger Environmental security, and its ion produced has Strong oxdiative
Effect, has corrosiveness to equipment, and also have certain injury to human body.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of high-performance bio deodorization composite bacteria agent.
The second object of the present invention there is provided the preparation of the biological deodorizing composite bacteria agent.
The third object of the present invention there is provided the specifically used of the biological deodorizing composite bacteria agent.
The present invention is achieved through the following technical solutions：
A kind of biological deodorizing composite bacteria agent, including calculate in parts by mass,
Pseudomonas microbial inoculum（Pseudomonas）50-100 parts
Bacillus microbial inoculum（Bacillus）25-80 parts
Trichoderma viride belongs to microbial inoculum（Trichoderma viride）20-50 parts
Streptomyces microbial inoculum（Streptomyces）20-30 parts.
The preparation method of the biological deodorizing composite bacteria agent, specific preparation process is as follows：
（1）Sampling and preparation culture medium
Liquid manure is obtained to sample diluting liquid with sterilized water gradient dilution as standby, configuration good corresponding bacteria culture media, fungi
Culture medium and unwrapping wire bacterium culture medium；
（2）Strain separating
First sample diluting liquid is drawn respectively and is coated on flat board bacteria culture media, fungi culture medium and unwrapping wire bacterium culture medium, is inoculated with
After be inverted into culture in constant incubator, observation be sampled after 24 hours, and selects bacterial strain counted, then selected not
Inclined-plane line culture is carried out with bacterial strain, and numbers preservation；
（3）Strain primary dcreening operation
By step（2）Isolated strain bacterial strain is seeded on corresponding fluid nutrient medium respectively, is cultivated 48 hours；Then take training
Each fluid nutrient medium after supporting is put into test tube, and instills nessler reagent, by the depth of the brownish red color of appearance or is whether there is
To judge that microorganism produces ammonia ability, the shallow bacterial strain of no color change or color change illustrates that the bacterial strain power production capacity is weak, with this
Filter out bacterial strain, number record；
（4）Bacterial strain presses down ammonia ability secondary screening
Fresh liquid manure is chosen, excrement slag is filtered and adds after glucose dissolving, be put into wide-mouth bottle as experiment matrix, connect
And use rubber stopper seal bottleneck, ammonia is absorbed with 2% boric acid solution by grass tube；
Respectively take corresponding step（3）The culture medium bacterium solution that middle primary dcreening operation comes out adds corresponding experiment matrix；It is put into shaking table vibration case
Middle to be cultivated, temperature is 37 DEG C, shaking table speed 60r/min；Boric acid solution is changed every 48h, and is dripped with dilution heat of sulfuric acid
Devise a stratagem calculates amonia yield, so that it is determined that the suppression ammonia ability of each bacterial strain, selects 5 days suppression ammonia ability bacterial strains more than 70%；
（5）Bacterial strain is identified and prepared by composite bacteria agent
First to step（4）The bacterial strain that middle secondary screening comes out is enlarged culture, 30 DEG C of cultivation temperature, shaking table speed 120r/min, training
Support time 48-72h；Then high speed centrifugation is carried out to the high concentration strain cultured solution that culture is obtained, collects thalline and be freeze-dried
Make bacterium powder；By bacterium powder and activated carbon powder according to 1:1 mass ratio is mixed, and single microbial inoculum is made；Finally by single microbial inoculum
The biological deodorizing composite bacteria agent is mixed to prepare according to a certain percentage.
It is preferred that in isolation medium used, bacteria culture media is：Beef extract 3g/L, peptone 10g/L, sodium chloride 5g/
L, agar 5g/L, pH value is 7.0-7.5；
Fungi culture medium is：Peptone 5g/L, glucose 10g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.5g/L, agar 5g/L,
1/3000 rose-bengal liquor capacity is 7.0-7.5 than 10%, chloramphenicol 0.1g/L, pH value；
Unwrapping wire bacterium culture medium is：Starch 20g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride
0.5g/L, ferrous sulfate 0.01g/L, agar 5g/L, pH value is 7.0-7.5.
It is preferred that step（3）The cultivation temperature of strain separating, 37 DEG C of bacteria culture media, actinomyces and fungi culture medium are
30 DEG C, cultivate 24h.
It is preferred that step（4）Strain primary dcreening operation, is cultivated 48 hours, 37 DEG C of cultivation temperature bacterium, and actinomyces and fungi are 30
DEG C, shaking table speed 120r/min.
Wherein, step（5）In the used culture medium that expands be respectively：
Bacterium expands culture medium：Beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, pH value are 7.0-7.5；Cultivation temperature
37 DEG C, shaking table speed 120r/min；
Mould expands culture medium：20% potato leaching juice, glucose 20g/L, pH value are 7.0-7.5；
Actinomyces expand culture medium：Starch 20g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, chlorine
Change sodium 0.5g/L, ferrous sulfate 0.01g/L, agar 5g/L, pH value is 7.0-7.5.
The biological deodorizing composite bacteria agent is used to handle Domestic kitchen waste, garbage is fitted into Plastic Drum,
Biological deodorizing composite bacteria agent described in last layer is spread on its surface, and input amount is 8%-15%.After stirring with hermetic bag sealing simultaneously
Be placed in bottom behind warm place, 1-2 weeks and zymotic fluid occur, can be used as liquid fertilizer, the garbage fermented without obvious peculiar smell,
It can place into the soil to do and continue to become thoroughly decomposed as fertilizer.
Wherein, biological deodorizing composite bacteria agent includes what is calculated in parts by mass, 50-60 parts of pseudomonas microbial inoculum, gemma
70-80 parts of Bacillus microbial inoculum, Trichoderma viride belongs to 40-50 parts of microbial inoculum, 20-30 parts of streptomyces microbial inoculum.
The biological deodorizing composite bacteria agent is used to handle excrement of animals rubbish, and composite bacteria agent is put into livestock pond daily covers
Lid manure pit surface, 8%-20%；After one week, pig manure pond and peculiar smell has obvious mitigation before not applying.Wherein, the biology
Deodorization composite bacteria agent includes what is calculated in parts by mass, 80-100 parts of pseudomonas microbial inoculum, bacillus microbial inoculum 25-50
Part, Trichoderma viride belongs to 20-30 parts of microbial inoculum, 20-30 parts of streptomyces microbial inoculum.
The present invention by from excrement culture of isolated obtain and press down pseudomonas microbial inoculum of the ammonia ability more than 70%
（Pseudomonas）, bacillus microbial inoculum（Bacillus）, Trichoderma viride belong to microbial inoculum（Trichoderma viride）, chain
Mould belongs to microbial inoculum（Streptomyces）Several bacterial strains are compounded according to optimal collaboration suppression ammonia effect ratio between them, are passed through
Absorption and conversion of the microorganism to ammonia produce so as to reduce ammonia.
The present invention is by separating and screening the various microorganisms in septic tank low-level culture pool in fermentation liquid manure（Bacterium, actinomyces,
Fungi）, isolate and obtain 96 kinds of bacterial strains, wherein 53 plants of bacterium, 23 plants of actinomyces, 20 plants of fungi.Determined by producing ammonia experiment
There are 21 plants, wherein 12 plants of bacterium, 5 plants of actinomyces, 4 plants of fungi with the bacterial strain for suppressing ammonia generation.21 plants of bacterial strains are inoculated with respectively
To simulation liquid manure experiment, it is found that its 18 plants have an obvious suppression ammonia effect, and 5 plants of suppression ammonia rate is up to more than 70%, will
5 plants of bacterial strains carry out bacterial strain identification, and it is pseudomonas as a result to show 2 plants（Pseudomonas）, 1 plant is bacillus
（Bacillus）, 1 plant is that Trichoderma viride belongs to（Trichoderma viride）, 1 plant is streptomyces（Streptomyces）.With
Each single microbial inoculum is made after 5 kinds of bacterial strains are enlarged into culture afterwards, and mixes composite bacteria agent according to a certain percentage, pig is actually used in
Peculiar smell removes and all achieves good result in the manure pit deodorization of house and kitchen garbage.Wherein, septic tank is by sanitary sewage lattice
Precipitation, the small primary environmental protection treatment structures of anaerobic digestion are carried out to liquid dung sludge.Typically together designed with building, together
Construct, together check and accept and use.Its operation principle is（By taking three-grid septic tank as an example）：Solid waste combined sewage enters the first lattice, than
The precipitation such as the larger solids of weight and parasitic ovum, anaerobic fermentation and decomposition in pond, sewage is divided into three layers：Pasty state excrement skin,
Liquid manure, the excrement slag of solid-like of relative clarification.
In low-level culture pool by fermentation after, COD and ammonia nitrogen are very high in liquid manure, strain group the most enrich, each microbial population
It is stable, it is the ideal source for obtaining deodorization functions strain.
In a word, the present invention is used for deodorization, and wide adaptability, toxigenic capacity are low, easy to use, deodorizing effect is good.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, is managed to help those skilled in the art
The solution present invention.
Embodiment 1
It is applied to the composite bacteria agent of the pig manure deodorization of pig house, including 100 parts of pseudomonas microbial inoculum, bacillus microbial inoculum 30
Part, Trichoderma viride belongs to 20 parts of microbial inoculum, 20 parts of streptomyces microbial inoculum,
When specifically applying：As composite bacteria agent after several microbial inoculums are mixed, the pig manure deodorization of pig house is applied to.Daily to pig manure pond
Middle input composite bacteria agent covering manure pit surface, input amount is calculated as 15% by mass percentage.After one week, pig manure pond and not
Peculiar smell has obvious mitigation before application.
Embodiment 2
It is applied to the composite bacteria agent of the pig manure deodorization of pig house, including 80 parts of pseudomonas microbial inoculum, bacillus microbial inoculum 50
Part, Trichoderma viride belongs to 20 parts of microbial inoculum, 20 parts of streptomyces microbial inoculum,
When specifically applying：Be the same as Example 1, input amount is calculated as 8% by mass percentage.After one week, pig manure pond and do not apply it
Preceding peculiar smell has obvious mitigation.
Embodiment 3
It is applied to the composite bacteria agent of the pig manure deodorization of pig house, including 85 parts of pseudomonas microbial inoculum, bacillus microbial inoculum 25
Part, Trichoderma viride belongs to 30 parts of microbial inoculum, 30 parts of streptomyces microbial inoculum,
When specifically applying：Be the same as Example 1, input amount is calculated as 10% by mass percentage.After one week, pig manure pond and do not apply it
Preceding peculiar smell has obvious mitigation.
Embodiment 4
It is applied to the composite bacteria agent of household kitchen wastes deodorization, including 60 parts of pseudomonas microbial inoculum, bacillus microbial inoculum
70 parts, Trichoderma viride belongs to 50 parts of microbial inoculum, 20 parts of streptomyces microbial inoculum,
When specifically applying：After being mixed after several microbial inoculums are mixed as composite bacteria agent household kitchen garbage rubbish is applied to as composite bacteria agent
The deodorization of rubbish.Rubbish from cooking is fitted into Plastic Drum, last layer bacterium powder is spread on its surface, input amount is 10%, and is stirred,
Bottom behind warm place, 1-2 weeks is sealed and placed in hermetic bag zymotic fluid occurs, can used as liquid fertilizer, the food waste fermented
Rubbish can place into the soil to do and continue to become thoroughly decomposed as fertilizer without obvious peculiar smell.
Embodiment 5
It is applied to the composite bacteria agent of household kitchen wastes deodorization, including 50 parts of pseudomonas microbial inoculum, bacillus microbial inoculum
80 parts, Trichoderma viride belongs to 40 parts of microbial inoculum, 30 parts of streptomyces microbial inoculum,
When specifically applying：Be the same as Example 4, input amount is about 20%.
Embodiment 6
It is applied to the composite bacteria agent of household kitchen wastes deodorization, including 55 parts of pseudomonas microbial inoculum, bacillus microbial inoculum
75 parts, Trichoderma viride belongs to 45 parts of microbial inoculum, 25 parts of streptomyces microbial inoculum,
When specifically applying：Be the same as Example 4, input amount is about 8%.
The preparation method of the biological deodorizing composite bacteria agent：
1.1 sampling
It is random to take each 5ml of low-level culture pool liquid manure in 3 cell septic tanks, and 15ml mixing liquid manures are mixed to form, liquid manure is in brownish black,
COD is close to 12000, the meta-acid of pH value 6.5.
1.2 strain separating
15ml liquid manures are made 10 respectively with sterilized water-4、10-5、10-6、10-7、10-8Gradient dilution liquid is as standby.
Isolation medium used is：Beef-protein medium（Bacteria culture media）, composition is：Wherein beef extract 3g/
L, peptone 10g/L, sodium chloride 5g/L, agar 5g/L, pH value are neutrality（7.0-7.5）；
Martin-rose bengal medium（Fungi culture medium）, composition is：Peptone 5g/L, glucose 10g/L, potassium dihydrogen phosphate
1g/L, magnesium sulfate 0.5g/L, agar 5g/L, 1/3000 rose-bengal liquor capacity are than the g/L of 10%, chloramphenicol 0.1, and pH value is
It is neutral（7.0-7.5）；
Gause I culture medium（Unwrapping wire bacterium culture medium）, composition is：Starch 20g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/
L, magnesium sulfate 0.5g/L, sodium chloride 0.5g/L, ferrous sulfate 0.01g/L, agar 5g/L, pH value are neutrality（7.0-7.5）；
By 10-6、10-7、10-8Gradient dilution liquid is drawn 1ml and is coated on flat board bacteria culture media respectively, by 10-4、10-5、10-6Ladder
Degree dilution draw respectively 1ml be coated on flat board Martin-rose bengal medium and Gause I culture medium on, it is dilute after inoculation
Release liquid and apply uniform with special sterilizing spreading rod, and be inverted into constant incubator, 37 DEG C of cultivation temperature bacteria culture media, unwrapping wire
Bacterium and fungi culture medium are 30 DEG C, cultivate 24h.
Observation is sampled after 24h, and selects bacterial strain and is counted, isolates and obtains 96 kinds of bacterial strains, wherein 53 plants of bacterium,
23 plants of actinomyces, 20 plants of fungi.Then select different strains and carry out inclined-plane line culture, and number preservation.
1.3 strain primary dcreening operations
Above-mentioned 96 plants of isolated bacterial strains are seeded on corresponding fluid nutrient medium respectively, fluid nutrient medium, which is removed, is free of agar
Outside, other compositions are identical with isolation medium.37 DEG C of cultivation temperature bacterium, actinomyces and fungi are 30 DEG C, shaking table speed
120r/min, is cultivated 48 hours.Then take each fluid nutrient medium after culture to be put into test tube, and instill nessler reagent, pass through
The depth of the brownish red color of appearance whether there is to judge that microorganism produces ammonia ability, the shallow bacterium of no color change or color change
Strain illustrates that the bacterial strain power production capacity is weak.21 plants of bacterial strains are filtered out with this, wherein 12 plants of bacteriums, 5 fungal strains and 4 plants of actinomyces, compile
Number record.
1.4 bacterial strains press down ammonia ability secondary screening
Fluid nutrient medium used is that the suppression ammonia ability of bacterial strain needs to enter traveling one by simulated experiment used in the theoretical primary dcreening operation in laboratory
Step confirmation.The liquid manure that 500ml fresh pigs steep excrement is chosen, excrement slag is filtered and is put into 1000ml after adding the dissolving of 10g glucose
Wide-mouth bottle in as experiment matrix, blend rubber plug sealing bottleneck, pass through grass tube with 2% boric acid solution absorption ammonia.
For 21 plants of bacterial strains of primary dcreening operation, the culture medium bacterium solution of the corresponding primary dcreening operations of 5ml is respectively taken to add corresponding experiment matrix.Set one simultaneously
Control group, bacterium solution is not added with containing only experiment matrix.
Wide-mouth bottle is put into shaking table vibration case and cultivated, temperature is 37 DEG C, shaking table speed 60r/min.Every 48h more
Boric acid solution is changed, and calculating amonia yield is titrated with dilution heat of sulfuric acid, so that it is determined that the suppression ammonia ability of each bacterial strain, incubation time
Common 240h.
It is found through experiments that in above-mentioned 21 plants of bacterial strains there are 2 plants of actual suppression ammonia abilities to pig manure liquid unobvious, explanation
The bacterial strain screened by laboratory cultures base not can also have same effect in actual matrix.And remaining 18 plants have certain
Suppression ammonia ability, 3 plants of bacteriums therein, 1 plant of actinomyces and 1 fungal strain have obvious suppression ammonia ability to pig manure liquid, press down ammonia energy
Power is up to more than 70%.
As can be seen from the table, in the 192h of inoculation, ammonia amount rises always in control group pig manure liquid, illustrates microorganism to ammonia
Decompose and be in peak period, after 192h hours, ammonia rate of release slows down, illustrate that organic matter is gradually decreased or microorganism profit in matrix
Nitrate nitrogen speed is converted into ammonia and starts quickening, so as to slow down the burst size of ammonia.5 plants of bacterial strains of screening equal energy after addition
Ammonia burst size is significantly reduced, suppression ammonia ability illustrates that 5 plants of bacterial strains have good deodorization functions more than 70% within 5 days.
1.5 bacterial strains are identified and prepared by composite bacteria agent
By the way that 5 plants of bacterial strains are carried out into plate streaking culture, it can determine whether out that 2 plants are false single according to colonial morphology and physio-biochemical characteristics
Born of the same parents' Bacillus（Pseudomonas）, 1 plant is bacillus（Bacillus）, 1 plant is that Trichoderma viride belongs to（Trichoderma viride）, 1 plant is streptomyces（Streptomyces）.
Take 5ml to be put into volume ratio 50%-60% 3L fermentation tanks the fluid nutrient medium of 5 plants of bacterial strains of numbering to be expanded
Big culture, it is beef-protein medium that bacterium, which expands culture medium, and composition is：Wherein beef extract 3g/L, the g/L of peptone 10,
The g/L of sodium chloride 5, pH value are neutrality（7.0-7.5）；37 DEG C of cultivation temperature, shaking table speed 120r/min.
It is PDA culture medium that mould, which expands culture medium, and composition is：20% potato leaching juice, during glucose 20g/L, pH value are
Property（7.0-7.5）；30 DEG C of cultivation temperature, shaking table speed 120r/min.
It is Gause I culture medium that actinomyces, which expand culture medium, and composition is：Starch 20g/L, potassium nitrate 1g/L, phosphoric acid hydrogen two
Potassium 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 0.5g/L, ferrous sulfate 0.01g/L, agar 5g/L, pH value are neutrality（7.0-
7.5）.30 DEG C of cultivation temperature, shaking table speed 120r/min.Each strain culturing time 48-72h, until thalline in fermentation tank
Content >=109Individual/ml.
High speed centrifugation is carried out to above-mentioned high concentration strain cultured solution, thalline is collected and is freeze-dried and make bacterium powder.By bacterium powder
With activated carbon powder according to 1:1 mass ratio is mixed, and single microbial inoculum is made.Single microbial inoculum is mixed to prepare according to a certain percentage
Composite bacteria agent.
Above-described embodiment, simply presently preferred embodiments of the present invention, not for limiting the scope of the present invention, equally, its
The processing of his livestock stink may be referred to accompanying for each microorganism in swine manure treatment and adjust accordingly；Therefore it is all with right of the present invention will
The equivalent change or modification for asking described feature and principle to be done, all should be included within scope of the invention as claimed.
Claims (10)
1. a kind of biological deodorizing composite bacteria agent, it is characterised in that：Including what is calculated in parts by mass,
2. the preparation method of biological deodorizing composite bacteria agent as claimed in claim 1, it is characterised in that：Specific preparation process is as follows：
(1) sample and prepare culture medium
Liquid manure is obtained into sample diluting liquid with sterilized water gradient dilution, it is standby；Configuration good corresponding bacteria culture media, fungal culture
Base and unwrapping wire bacterium culture medium；
(2) strain separating
First sample diluting liquid is drawn respectively and is coated on flat board bacteria culture media, fungi culture medium and unwrapping wire bacterium culture medium, is inoculated with
After be inverted into culture in constant incubator, observation be sampled after 24 hours, and selects bacterial strain counted, then selected not
Inclined-plane line culture is carried out with bacterial strain, and numbers preservation；
(3) strain primary dcreening operation
The isolated strain bacterial strain of step (2) is seeded on corresponding fluid nutrient medium respectively, cultivated 48 hours；Then take training
Each fluid nutrient medium after supporting is put into test tube, and instills nessler reagent, by the depth of the brownish red color of appearance or is whether there is
To judge that microorganism produces ammonia ability, the shallow bacterial strain of no color change or color change illustrates that the bacterial strain power production capacity is weak, with this
Filter out bacterial strain, number record；
(4) bacterial strain suppression ammonia ability secondary screening
Fresh liquid manure is chosen, excrement slag is filtered and adds after glucose dissolving, be put into wide-mouth bottle as experiment matrix, connect
And use rubber stopper seal bottleneck, ammonia is absorbed with 2% boric acid solution by grass tube；
The culture medium bacterium solution that primary dcreening operation comes out in corresponding step (3) is respectively taken to add corresponding experiment matrix；It is put into shaking table vibration case
It is middle to be cultivated, shaking table speed 60r/min；Boric acid solution is changed every 48h, and calculating is titrated with dilution heat of sulfuric acid and produces ammonia
Amount, so that it is determined that the suppression ammonia ability of each bacterial strain, selects 5 days suppression ammonia ability bacterial strains more than 70%；
(5) prepared by bacterial strain identification and composite bacteria agent
First the several bacterial strains that secondary screening in step (4) comes out are respectively put into fermentation tank and are enlarged culture, 30 DEG C of cultivation temperature,
Shaking table speed 120r/min, incubation time 48-72h, until thalline content is more than or equal to 10 in fermentation tank9It is individual；Then to culture
Obtained high concentration strain cultured solution carries out high speed centrifugation, collects thalline and is freeze-dried and makes bacterium powder；By bacterium powder and activated carbon
Powder is according to 1:1 mass ratio is mixed, and single microbial inoculum is made；Finally single microbial inoculum is mixed to prepare according to a certain percentage described
Biological deodorizing composite bacteria agent.
3. the preparation method of biological deodorizing composite bacteria agent as claimed in claim 2, it is characterised in that：In isolation medium used,
Bacteria culture media is beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L and agar 5g/L, and pH value is 7.0-7.5；
Fungi culture medium is：Peptone 5g/L, glucose 10g/L, potassium dihydrogen phosphate 1g/L, magnesium sulfate 0.5g/L, agar 5g/L,
1/3000 rose-bengal liquor capacity is than 10% and chloramphenicol 0.1g/L, and pH value is 7.0-7.5；
Unwrapping wire bacterium culture medium is：Starch 20g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride
0.5g/L, ferrous sulfate 0.01g/L and agar 5g/L, pH value is 7.0-7.5.
4. the preparation method of biological deodorizing composite bacteria agent as claimed in claim 2, it is characterised in that：Step (3) strain separating
Cultivation temperature, 37 DEG C of bacteria culture media, actinomyces and fungi culture medium are 30 DEG C.
5. the preparation method of biological deodorizing composite bacteria agent as claimed in claim 2, it is characterised in that：Step (4) strain primary dcreening operation, training
It is 37 DEG C to support temperature bacterium, and actinomyces and fungi are 30 DEG C, shaking table speed 120r/min.
6. the preparation method of biological deodorizing composite bacteria agent as claimed in claim 2, it is characterised in that：It is used in step (5) to expand
Culture medium is respectively：
It is that beef extract 3g/L, peptone 10g/L and sodium chloride 5g/L, pH value are 7.0-7.5 that bacterium, which expands culture medium,；
Mould expands that culture medium is 20% potato leaching juice and glucose 20g/L, pH value are 7.0-7.5；
It is starch 20g/L, potassium nitrate 1g/L, dipotassium hydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, chlorination that actinomyces, which expand culture medium,
Sodium 0.5g/L, ferrous sulfate 0.01g/L and agar 5g/L, pH value is 7.0-7.5.
7. biological deodorizing composite bacteria agent described in any one in claims 1-6 is used to handle Domestic kitchen waste, it is special
Levy and be：Garbage is fitted into Plastic Drum, biological deodorizing composite bacteria agent described in last layer is spread on its surface, input amount presses matter
Amount percentage is calculated as 8%-15%.
8. the biological deodorizing composite bacteria agent as described in claims 7 is used to handle Domestic kitchen waste, it is characterised in that：It is described
Biological deodorizing composite bacteria agent includes what is calculated in parts by mass, 50-60 parts of pseudomonas microbial inoculum, bacillus microbial inoculum 70-
80 parts, Trichoderma viride belongs to 40-50 parts of microbial inoculum, 20-30 parts of streptomyces microbial inoculum.
9. biological deodorizing composite bacteria agent described in any one in claims 1-6 is used to handle excrement of animals rubbish, it is special
Levy and be：Composite bacteria agent covering manure pit surface is put into livestock pond daily, input amount is to calculate 8%- by mass percentage
20%.
10. the biological deodorizing composite bacteria agent as described in claims 9 is used to handle Domestic kitchen waste, it is characterised in that：Institute
Stating biological deodorizing composite bacteria agent includes what is calculated in parts by mass, 80-100 parts of pseudomonas microbial inoculum, bacillus microbial inoculum
25-50 parts, Trichoderma viride belongs to 20-30 parts of microbial inoculum, 20-30 parts of streptomyces microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611130257.4A CN107058153A (en) | 2016-12-09 | 2016-12-09 | A kind of biological deodorizing composite bacteria agent and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611130257.4A CN107058153A (en) | 2016-12-09 | 2016-12-09 | A kind of biological deodorizing composite bacteria agent and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107058153A true CN107058153A (en) | 2017-08-18 |
Family
ID=59619659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611130257.4A Pending CN107058153A (en) | 2016-12-09 | 2016-12-09 | A kind of biological deodorizing composite bacteria agent and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107058153A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107308810A (en) * | 2017-08-25 | 2017-11-03 | 广州普瑞泰克科技有限公司 | A kind of silty microbial inoculum for removing indoor formaldehyde and preparation method thereof |
| CN107952361A (en) * | 2017-11-13 | 2018-04-24 | 常州蓝旗亚纺织品有限公司 | A kind of preparation method of fowl and livestock farm deodorant |
| CN108102973A (en) * | 2018-01-31 | 2018-06-01 | 福建双环能源科技股份有限公司 | A kind of destructor plant percolate deodorization special bacterium and its application method |
| CN109837229A (en) * | 2019-04-11 | 2019-06-04 | 河北维尔利动物药业集团有限公司 | The preparation method of clostridium butyricum composite bacteria liquid for poultry |
| CN110964639A (en) * | 2019-12-09 | 2020-04-07 | 湖南金泥生物科技有限公司 | Strain screening method applied to pig raising fermentation bed and application |
| CN112608856A (en) * | 2020-11-19 | 2021-04-06 | 浙江延杭智能科技有限公司 | Compound microbial deodorant bacterial agent and preparation method and application thereof |
| CN114107069A (en) * | 2021-11-25 | 2022-03-01 | 广州市新之地环保产业股份有限公司 | Composite microbial agent for biological deodorization and preparation method and application thereof |
| CN114317359A (en) * | 2021-12-31 | 2022-04-12 | 云南中农俊发生物科技有限责任公司 | Microbial community compounding method with dual functions of deodorization and fermentation |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102327630A (en) * | 2011-09-15 | 2012-01-25 | 湖南省微生物研究所 | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof |
| CN103695331A (en) * | 2013-06-27 | 2014-04-02 | 深圳市新宝环保能源科技有限公司 | Bacillus coagulans and preparation method and application in biological deodorant thereof |
| CN104611258A (en) * | 2014-12-30 | 2015-05-13 | 青岛蔚蓝天成生物科技有限公司 | Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis |
| CN104774787A (en) * | 2015-03-26 | 2015-07-15 | 泰安市农业再生资源综合利用研究开发中心 | Compounding method of microorganism agent for chicken manure fermentation |
| CN105255761A (en) * | 2015-10-23 | 2016-01-20 | 武汉骏安生物科技有限公司 | Deodorization fungicide for treating organic waste materials and application method thereof |
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
-
2016
- 2016-12-09 CN CN201611130257.4A patent/CN107058153A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102327630A (en) * | 2011-09-15 | 2012-01-25 | 湖南省微生物研究所 | Microbial strain and deodorant for municipal landfill, and preparation and application method thereof |
| CN103695331A (en) * | 2013-06-27 | 2014-04-02 | 深圳市新宝环保能源科技有限公司 | Bacillus coagulans and preparation method and application in biological deodorant thereof |
| CN104611258A (en) * | 2014-12-30 | 2015-05-13 | 青岛蔚蓝天成生物科技有限公司 | Bacillus subtilis and biological deodorant containing bacterial strain of bacillus subtilis |
| CN104774787A (en) * | 2015-03-26 | 2015-07-15 | 泰安市农业再生资源综合利用研究开发中心 | Compounding method of microorganism agent for chicken manure fermentation |
| CN105255761A (en) * | 2015-10-23 | 2016-01-20 | 武汉骏安生物科技有限公司 | Deodorization fungicide for treating organic waste materials and application method thereof |
| CN105384480A (en) * | 2015-11-16 | 2016-03-09 | 北京嘉博文生物科技有限公司 | Biological humic acid organic material decomposition agent having deodorization function |
Non-Patent Citations (4)
| Title |
|---|
| KANANI ET AL.: "Organic and Inorganic Amendments to Reduce Ammonia Losses from Liquid Hog Manure.", 《J ENVIRON QUAL》 * |
| 张生伟等: "猪粪高效除臭微生物菌株筛选及发酵条件优化", 《草业学报》 * |
| 曾晰菀: "猪粪堆肥高温除臭复合菌剂的选育及应用", 《中国优秀硕士学位论文全文数据库，农业科技辑》 * |
| 顾文杰: "禽粪好氧堆肥除臭菌筛选及其促腐熟效果研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107308810A (en) * | 2017-08-25 | 2017-11-03 | 广州普瑞泰克科技有限公司 | A kind of silty microbial inoculum for removing indoor formaldehyde and preparation method thereof |
| CN107952361A (en) * | 2017-11-13 | 2018-04-24 | 常州蓝旗亚纺织品有限公司 | A kind of preparation method of fowl and livestock farm deodorant |
| CN108102973A (en) * | 2018-01-31 | 2018-06-01 | 福建双环能源科技股份有限公司 | A kind of destructor plant percolate deodorization special bacterium and its application method |
| CN109837229A (en) * | 2019-04-11 | 2019-06-04 | 河北维尔利动物药业集团有限公司 | The preparation method of clostridium butyricum composite bacteria liquid for poultry |
| CN110964639A (en) * | 2019-12-09 | 2020-04-07 | 湖南金泥生物科技有限公司 | Strain screening method applied to pig raising fermentation bed and application |
| CN112608856A (en) * | 2020-11-19 | 2021-04-06 | 浙江延杭智能科技有限公司 | Compound microbial deodorant bacterial agent and preparation method and application thereof |
| CN114107069A (en) * | 2021-11-25 | 2022-03-01 | 广州市新之地环保产业股份有限公司 | Composite microbial agent for biological deodorization and preparation method and application thereof |
| CN114317359A (en) * | 2021-12-31 | 2022-04-12 | 云南中农俊发生物科技有限责任公司 | Microbial community compounding method with dual functions of deodorization and fermentation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107058153A (en) | A kind of biological deodorizing composite bacteria agent and its preparation method and application | |
| CN102557772B (en) | Fermentation device for sludge compost treatment and treatment method using same | |
| CN109182193A (en) | Microbial bacterial agent and its preparation method and application | |
| CN102093975B (en) | Composite fungicide for rapid degradation of organic waste and applications thereof | |
| CN101289637B (en) | Method for preparing clean fuel derived from sludge | |
| CN103977704B (en) | A method of deodorization being carried out to rubbish using complex micro organism fungicide | |
| CN101244955A (en) | Biological fertilizer and manufacture method thereof | |
| CN101333510A (en) | Method for processing sludge and preparing bio organic fertilizer and special leaven thereof | |
| CN105062919A (en) | Microbial agent for treatment of sewage and sludge | |
| CN114988926A (en) | Organic compound fertilizer produced by eighty percent of sludge with water content and manufacturing method thereof | |
| CN110090851A (en) | A kind of manufacturing method quickly handling domestic waste and derivative with high temperature | |
| CN109796113A (en) | A kind of sludge and derivative manufacturing method of high speed processing moisture content 80 percent | |
| CN106701628A (en) | Rural household refuse fermentation microbial inoculum and using method and application thereof | |
| CN103981138B (en) | A kind of preparation method of microbiobacterial agent and the microbiobacterial agent obtained and application | |
| CN106754572A (en) | One bacillus amyloliquefaciens and application thereof | |
| CN108148790A (en) | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation | |
| CN110981563A (en) | Treatment method and application of antibiotic fungi residues | |
| CN101215532A (en) | Bacillus megaterium and its application and application method in ferment bacteria | |
| CN108192845B (en) | Enzyme-bacterium complexing agent for harmless treatment of animal carcasses and preparation method thereof | |
| CN105820976A (en) | Sludge compost thermophilic microbial agent and application | |
| JP3064221B2 (en) | Aerobic bacteria and sludge treatment using the same | |
| CN105296355A (en) | Environmental microorganism bacterium extraction and propagation compositions and using methods thereof | |
| CN108531412A (en) | A kind of preparation method of ECMD Efficient Rings control microbial deodorant | |
| CN102727920B (en) | Deodorant of paper sludge and urban living garbage biomass environment-friendly renewable energy, and preparation method thereof | |
| CN109266573B (en) | Fermentation agent and microbial inoculum for treating landfill leachate and reverse osmosis concentrated solution and treatment method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200720 Address after: 29d, Jinghui Pavilion, Jingxin garden, No. 2002, Lianhua Road, Futian District, Shenzhen City, Guangdong Province Applicant after: Luo Zhenjie Address before: The streets of Shenzhen city in Guangdong Province, 518000 Station Road, Luohu District No. 8 Qingshui River Road No. 1 Yiyuan Lily Applicant before: 301 (SHENZHEN) BIOLOGICAL ENVIRONMETAL PROTECTION Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170818 |
|
| RJ01 | Rejection of invention patent application after publication |