CN114317359A - Microbial community compounding method with dual functions of deodorization and fermentation - Google Patents
Microbial community compounding method with dual functions of deodorization and fermentation Download PDFInfo
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- CN114317359A CN114317359A CN202111666333.4A CN202111666333A CN114317359A CN 114317359 A CN114317359 A CN 114317359A CN 202111666333 A CN202111666333 A CN 202111666333A CN 114317359 A CN114317359 A CN 114317359A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a flora compounding method with double functions of deodorization and fermentation, which belongs to the technical field of fertilizer production.
Description
Technical Field
The invention belongs to the technical field of fertilizer production, and particularly relates to a microbial community compounding method with dual functions of deodorization and fermentation.
Background
The organic fertilizer is also called 'farmyard manure'. Organic fertilizers, which are organic substances (compounds containing carbon elements), include human excrement, animal manure, compost, green manure, cake manure, biogas manure, and the like. The organic fertilizer has the characteristics of multiple types, wide sources, long fertilizer efficiency and the like, the nutrient elements contained in the organic fertilizer are mostly in an organic state, the crops are difficult to directly utilize, the various nutrient elements are slowly released under the action of microorganisms, the nutrients are continuously supplied to the crops, the soil structure can be improved by applying the organic fertilizer, the water, fertilizer, gas and heat in the soil are coordinated, the soil fertility and the land productivity are improved, the use value of the organic fertilizer is high, but the odor of the organic fertilizer is serious and affects the environment, the operating environment of operators in the production process is poor, the odor source in the production process of the organic fertilizer is the raw material rotting and deterioration, the odor generated by fermentation and drying of the organic fertilizer is large, the sources are wide, the ammonia odor, the hydrogen sulfide odor and the like generally cause toxic hazard to human breathing, digestion, cardiovascular, endocrine and nervous systems in different degrees, the production process of the organic fertilizer is generally matched with a deodorization system, and other deodorization methods are matched for use, such as a water washing method, an air dilution method, a masking method, a biological deodorization method and the like, wherein the biological deodorization method achieves the aim of deodorization through microbial decomposition and odor conversion under the action of organisms, and the biological deodorization method is low in cost and can effectively save energy; at present, the organic fertilizer biological deodorization is realized by firstly deodorizing by adopting a deodorant and then fermenting by adding zymophyte, the deodorization effect is not ideal, the main reason is incomplete decomposition of putrefactive protein, the pertinence of the deodorant is not strong, in the existing deodorization and fermentation technology, the pertinence of the deodorant is strong, the odor on the surface can be pressed after the deodorant is added, the deodorization cannot be deeply performed, once the odor is volatilized by turning over the material, and the labor cost is increased by adding the deodorant twice.
Disclosure of Invention
In order to overcome the problems in the background art, the invention discloses a method for compounding deodorant and fermentation dual-functional flora, the method for compounding the deodorant and fermentation dual-functional flora is used for compounding to obtain two bacterium agents, the compound bacterium agent can be used for fermenting the organic fertilizer by using the two compounded bacterium agents, the two bacterium agents are matched to effectively remove the odor of the organic fertilizer, the quality of the production process is controllable, the proportion of the produced flora is stable, the deodorization effect is good, fermentation is realized at the same time when the two bacterium agents are added once, the deodorization function is good, and the time and the labor are saved.
In order to realize the purpose, the invention is realized by the following technical scheme:
the deodorization fermentation dual-function flora compounding method comprises the following steps:
1) single strain propagation: separately propagating bacillus, trichoderma and actinomycetes;
2) propagation of archaea: respectively extracting archaea at 80-100 deg.C, pH1.0-2.5 and pH9-10, and performing propagation by purifying the extracted archaea, rejuvenating, and performing propagation at 80-100 deg.C;
3) preparing balance bacteria: utilizing bacillus, trichoderma and actinomycetes to mix and propagate to prepare balance bacteria, utilizing a metabolite principle to inhibit the dominant flora in the bacillus, trichoderma and actinomycetes, and preventing the flora proportion from being influenced by excessive growth and the flora from being disordered;
4) compounding: compounding bacillus, trichoderma and actinomycetes, and adding balancing bacteria.
Preferably, the method comprises the following steps: in the step 1), the single strain propagation operation is to propagate bacillus, trichoderma and actinomycetes respectively by using microorganism propagation equipment.
Preferably, the method comprises the following steps: and (3) in the step 2), the archaea can be propagated by using microorganism propagation equipment in the step 2).
Preferably, the method comprises the following steps: the archaea purified in the step 2) is high-temperature thermophilic bacteria, acidophilic bacteria and basophil extracted in natural environment, and extreme thermophilic bacteria, extreme acidophilic bacteria and extreme basophil are obtained by purifying the high-temperature thermophilic bacteria, acidophilic bacteria and basophil.
Preferably, the method comprises the following steps: in the step 2), the extreme thermophilic bacteria in the archaea are high-temperature thermophilic bacteria extracted from a high-temperature hot spring outlet or near a volcanic vent are cultured on a PDA culture medium with the pH value of 6-7, the PDA culture medium is placed on a water bath kettle, the extreme thermophilic bacteria are obtained by propagation under the condition that the temperature is kept at 80-100 ℃, and the extreme thermophilic bacteria are: culturing extreme thermophilic bacteria and acidophilic bacteria obtained from an acid chemical raw material stacking site on a PDA culture medium with the pH value of 1.0-2.5, and culturing and propagating to obtain strains, wherein the extreme alkalophilic bacteria are: culturing extreme thermophilic bacteria and alkalophilic bacteria obtained from an alkaline chemical raw material stacking site on a PDA culture medium with the pH value of 9-10, culturing and propagating the obtained strains, and then performing strain compounding.
Preferably, the method comprises the following steps: the strain compounding is that 30 percent of extreme thermophilic bacteria, 30 percent of extreme acidophilic bacteria, 30 percent of extreme alkalophilic bacteria and 10 percent of balancing bacteria are compounded into an archaea flora.
Preferably, the method comprises the following steps: the compounding in the step 4) comprises the following steps:
(1) preparing a culture medium: adding starch, peptone, beef extract, glucose, amino acids, vitamins, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride into warm water of 50-60 ℃, fully stirring until completely dissolving, and then cooling to 35-45 ℃ to obtain a liquid culture medium;
(2) and (3) mixing the microbial inoculum: mixing 30% of bacillus, 30% of actinomycetes, 30% of trichoderma and 10% of balance bacteria into the liquid culture medium in the step (1) to form a liquid microbial inoculum.
Preferably, the archaea flora is inoculated into a fertilizer.
Preferably, the method comprises the following steps: the liquid microbial inoculum is inoculated into a fertilizer. .
The invention has the beneficial effects that: the deodorization and fermentation dual-function flora compounding method obtains two consistent microbial agents through compounding, the compound microbial agent can be used for fermenting the organic fertilizer by using the two compounded microbial agents, the odor of the organic fertilizer can be effectively removed through the matching treatment of the two microbial agents, the quality in the production process is controllable, the proportion of the mass-stable production flora is stable, the deodorization effect is good, fermentation is realized through one-time addition, the deodorization function is good, and time and labor are saved.
Detailed Description
In order to make the above objects, technical solutions and advantages clearer and more clear, the present invention is specifically described below with reference to the following embodiments.
Example 1
The fertilizer deodorant is compounded by using the deodorizing and fermenting dual-function flora compounding method, and the deodorizing and fermenting dual-function flora compounding method comprises the following steps:
1) propagation and compounding of single strain: separately propagating bacillus, trichoderma and actinomycetes, respectively propagating the bacillus, the trichoderma and the actinomycetes by utilizing microorganism propagation equipment, compounding the bacillus, the trichoderma and the actinomycetes, and adding balancing bacteria, wherein the compounding operation comprises (1) preparing a culture medium: adding starch, peptone, beef extract, glucose, amino acid, vitamins, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride into warm water at 50-60 ℃, fully stirring until completely dissolving, then cooling to 35-45 ℃ to obtain a liquid culture medium, and (2) mixing microbial inoculum: mixing 30% of bacillus, 30% of actinomycetes, 30% of trichoderma and 10% of balance bacteria into the liquid culture medium in the step (1) to form a liquid microbial inoculum; inoculating the liquid microbial inoculum into a fertilizer;
2) propagation and compounding of archaea: extracting archaea under the conditions of 80-100 ℃, pH1.0-2.5 and pH9-10 respectively for propagation, wherein the propagation operation is to purify the extracted archaea, and propagate the archaea under the conditions of 80-100 ℃ after rejuvenation, the propagation of the archaea can utilize a microorganism propagation device to propagate the archaea, the purified archaea is high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria extracted in a natural environment, the high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria are purified to obtain extreme thermophilic bacteria, extreme acidophilic bacteria and extreme basophilic bacteria, the extreme thermophilic bacteria in the archaea are high-temperature thermophilic bacteria extracted from a high-temperature spring outlet or near a volcanic entrance, are cultured on a PDA culture medium with the pH value of 6-7 and are placed on a water bath kettle, and the extreme thermophilic bacteria are propagated under the condition of keeping the temperature of 80-100 ℃, the extreme acidophilic bacteria are: culturing extreme thermophilic bacteria and acidophilic bacteria obtained from an acid chemical raw material stacking site on a PDA culture medium with the pH value of 1.0-2.5, and culturing and propagating to obtain strains, wherein the extreme alkalophilic bacteria are: the extreme thermophilic bacteria and the alkalophilic bacteria obtained from an alkaline chemical raw material stacking site are cultured on a PDA culture medium with the pH value of 9-10, the strains obtained by propagation are cultured and propagated, then strain compounding is carried out, 30% of extreme thermophilic bacteria, 30% of extreme acidophilic bacteria, 30% of extreme alkalophilic bacteria and 10% of balancing bacteria are compounded into archaea flora, and the archaea flora is inoculated into the fertilizer.
Example 2
The fertilizer deodorant is compounded by using the deodorizing and fermenting dual-function flora compounding method, and the deodorizing and fermenting dual-function flora compounding method comprises the following steps:
1) propagation and compounding of single strain: separately propagating bacillus, trichoderma and actinomycetes, respectively propagating the bacillus, the trichoderma and the actinomycetes by utilizing microorganism propagation equipment, compounding the bacillus, the trichoderma and the actinomycetes, and adding balancing bacteria, wherein the compounding operation comprises (1) preparing a culture medium: adding starch, peptone, beef extract, glucose, amino acid, vitamins, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride into warm water at 50-60 ℃, fully stirring until completely dissolving, then cooling to 35-45 ℃ to obtain a liquid culture medium, and (2) mixing microbial inoculum: mixing 40% of bacillus, 30% of actinomycetes and 30% of trichoderma into the liquid culture medium in the step (1) to form a liquid microbial inoculum; inoculating the liquid microbial inoculum into a fertilizer;
2) propagation and compounding of archaea: extracting archaea under the conditions of 80-100 ℃, pH1.0-2.5 and pH9-10 respectively for propagation, wherein the propagation operation is to purify the extracted archaea, and propagate the archaea under the conditions of 80-100 ℃ after rejuvenation, the propagation of the archaea can utilize a microorganism propagation device to propagate the archaea, the purified archaea is high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria extracted in a natural environment, the high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria are purified to obtain extreme thermophilic bacteria, extreme acidophilic bacteria and extreme basophilic bacteria, the extreme thermophilic bacteria in the archaea are high-temperature thermophilic bacteria extracted from a high-temperature spring outlet or near a volcanic entrance, are cultured on a PDA culture medium with the pH value of 6-7 and are placed on a water bath kettle, and the extreme thermophilic bacteria are propagated under the condition of keeping the temperature of 80-100 ℃, the extreme acidophilic bacteria are: culturing extreme thermophilic bacteria and acidophilic bacteria obtained from an acid chemical raw material stacking site on a PDA culture medium with the pH value of 1.0-2.5, and culturing and propagating to obtain strains, wherein the extreme alkalophilic bacteria are: the extreme thermophilic bacteria and the alkalophilic bacteria obtained from an alkaline chemical raw material stacking site are cultured on a PDA culture medium with the pH value of 9-10, the strains obtained by propagation are cultured and expanded, then strain compounding is carried out, 30% of the extreme thermophilic bacteria, 40% of the extreme acidophilic bacteria and 30% of the extreme alkalophilic bacteria are compounded into archaic flora, and the archaic flora is inoculated into the fertilizer.
Example 3
The fertilizer deodorant is compounded by using the deodorizing and fermenting dual-function flora compounding method, and the deodorizing and fermenting dual-function flora compounding method comprises the following steps:
propagation and compounding of single strain: separately propagating bacillus, trichoderma and actinomycetes, respectively propagating the bacillus, the trichoderma and the actinomycetes by utilizing microorganism propagation equipment, compounding the bacillus, the trichoderma and the actinomycetes, and adding balancing bacteria, wherein the compounding operation comprises (1) preparing a culture medium: adding starch, peptone, beef extract, glucose, amino acid, vitamins, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride into warm water at 50-60 ℃, fully stirring until completely dissolving, then cooling to 35-45 ℃ to obtain a liquid culture medium, and (2) mixing microbial inoculum: mixing 30% of bacillus, 30% of actinomycetes, 30% of trichoderma and 10% of balance bacteria into the liquid culture medium in the step (1) to form a liquid microbial inoculum; the liquid microbial inoculum is inoculated into the fertilizer.
Example 4
Propagation and compounding of archaea: extracting archaea under the conditions of 80-100 ℃, pH1.0-2.5 and pH9-10 respectively for propagation, wherein the propagation operation is to purify the extracted archaea, and propagate the archaea under the conditions of 80-100 ℃ after rejuvenation, the propagation of the archaea can utilize a microorganism propagation device to propagate the archaea, the purified archaea is high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria extracted in a natural environment, the high-temperature thermophilic bacteria, acidophilic bacteria and basophilic bacteria are purified to obtain extreme thermophilic bacteria, extreme acidophilic bacteria and extreme basophilic bacteria, the extreme thermophilic bacteria in the archaea are high-temperature thermophilic bacteria extracted from a high-temperature spring outlet or near a volcanic entrance, are cultured on a PDA culture medium with the pH value of 6-7 and are placed on a water bath kettle, and the extreme thermophilic bacteria are propagated under the condition of keeping the temperature of 80-100 ℃, the extreme acidophilic bacteria are: culturing extreme thermophilic bacteria and acidophilic bacteria obtained from an acid chemical raw material stacking site on a PDA culture medium with the pH value of 1.0-2.5, and culturing and propagating to obtain strains, wherein the extreme alkalophilic bacteria are: the extreme thermophilic bacteria and the alkalophilic bacteria obtained from an alkaline chemical raw material stacking site are cultured on a PDA culture medium with the pH value of 9-10, the strains obtained by propagation are cultured and propagated, then strain compounding is carried out, 30% of extreme thermophilic bacteria, 30% of extreme acidophilic bacteria, 30% of extreme alkalophilic bacteria and 10% of balancing bacteria are compounded into archaea flora, and the archaea flora is inoculated into the fertilizer.
The fertilizers after fermentation in examples 1, 2, 3 and 4 were sampled and tested, the volatilization amount of ammonia gas and the content of sulfide in the fertilizers with the same quality were tested by a conventional method, and the test results show that the volatilization amount of ammonia gas is the lowest and the content of sulfide in the fertilizers with the lowest quality in example 1, the content of sulfide in the fertilizers with ammonia gas volatilization level in example 1 is lower than that in example 2, the content of sulfide in the fertilizers with ammonia gas volatilization level in example 2 is lower than that in example 4, and the content of sulfide in the fertilizers with ammonia volatilization level in example 4 is lower than that in example 3.
The deodorization and fermentation dual-function flora compounding method obtains two consistent microbial agents through compounding, the compound microbial agent can be used for fermenting the organic fertilizer by using the two compounded microbial agents, the odor of the organic fertilizer can be effectively removed through the matching treatment of the two microbial agents, the quality in the production process is controllable, the proportion of the mass-stable production flora is stable, the deodorization effect is good, fermentation is realized through one-time addition, the deodorization function is good, and time and labor are saved.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (9)
1. A method for compounding deodorant and fermentative dual-function flora is characterized by comprising the following steps:
1) single strain propagation: separately propagating bacillus, trichoderma and actinomycetes;
2) propagation of archaea: respectively extracting archaea at 80-100 deg.C, pH1.0-2.5 and pH9-10, and performing propagation by purifying the extracted archaea, rejuvenating, and performing propagation at 80-100 deg.C;
3) preparing balance bacteria: preparing balance bacteria by mixed propagation of bacillus, trichoderma and actinomycetes;
4) compounding: compounding bacillus, trichoderma and actinomycetes, and adding balancing bacteria.
2. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 1, which is characterized in that: in the step 1), the single strain propagation operation is to propagate bacillus, trichoderma and actinomycetes respectively by using microorganism propagation equipment.
3. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 1, which is characterized in that: and (3) in the step 2), the archaea can be propagated by using microorganism propagation equipment in the step 2).
4. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 1, which is characterized in that: the archaea purified in the step 2) is high-temperature thermophilic bacteria, acidophilic bacteria and basophil extracted in natural environment, and extreme thermophilic bacteria, extreme acidophilic bacteria and extreme basophil are obtained by purifying the high-temperature thermophilic bacteria, acidophilic bacteria and basophil.
5. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 4, which is characterized in that: in the step 2), the extreme thermophilic bacteria in the archaea are high-temperature thermophilic bacteria extracted from a high-temperature hot spring outlet or near a volcanic vent are cultured on a PDA culture medium with the pH value of 6-7, the PDA culture medium is placed on a water bath kettle, the extreme thermophilic bacteria are obtained by propagation under the condition that the temperature is kept at 80-100 ℃, and the extreme thermophilic bacteria are: culturing extreme thermophilic bacteria and acidophilic bacteria obtained from an acid chemical raw material stacking site on a PDA culture medium with the pH value of 1.0-2.5, and culturing and propagating to obtain strains, wherein the extreme alkalophilic bacteria are: culturing extreme thermophilic bacteria and alkalophilic bacteria obtained from an alkaline chemical raw material stacking site on a PDA culture medium with the pH value of 9-10, culturing and propagating the obtained strains, and then performing strain compounding.
6. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 6, which is characterized in that: the strain compounding is that 30 percent of extreme thermophilic bacteria, 30 percent of extreme acidophilic bacteria, 30 percent of extreme alkalophilic bacteria and 10 percent of balancing bacteria are compounded into an archaea flora.
7. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 1, which is characterized in that: the compounding in the step 4) comprises the following steps:
(1) preparing a culture medium: adding starch, peptone, beef extract, glucose, amino acids, vitamins, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride into warm water of 50-60 ℃, fully stirring until completely dissolving, and then cooling to 35-45 ℃ to obtain a liquid culture medium;
(2) and (3) mixing the microbial inoculum: mixing 30% of bacillus, 30% of actinomycetes, 30% of trichoderma and 10% of balance bacteria into the liquid culture medium in the step (1) to form a liquid microbial inoculum.
8. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 7, wherein the method comprises the following steps: the archaea flora is inoculated into a fertilizer.
9. The method for compounding the deodorizing and fermenting bifunctional flora according to claim 8, wherein the method comprises the following steps: the liquid microbial inoculum is inoculated into a fertilizer.
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