CN107308810A - A kind of silty microbial inoculum for removing indoor formaldehyde and preparation method thereof - Google Patents
A kind of silty microbial inoculum for removing indoor formaldehyde and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of silty microbial inoculum for removing indoor formaldehyde and preparation method thereof.The silty microbial inoculum, by mass percentage, including following component：Effective bacterium powder 2 ~ 3%, carbon source 20 ~ 25%, nitrogen source 45 ~ 50%, inorganic salts 5 ~ 7%, stabilizer 20 ~ 25%.The component is well mixed by the mass percent, that is, obtains the silty microbial inoculum of the removal indoor formaldehyde.The silty microbial inoculum of the present invention has the advantages that easy to use, shelf lives length, except formaldehyde efficiency high and pollution-free, effect stability.The preparation method of the present invention is simple, it is easy to accomplish industrialization.
Description
Technical field
The present invention relates to formaldehyde remove environmental technology field, and in particular to a kind of silty microbial inoculum of removal indoor formaldehyde and
Its preparation method.
Background technology
Formaldehyde, is commonly called as formaldehyde, is at normal temperatures the gas of colourless, irritant smell.Formaldehyde is industrially a kind of good
Good chemical solvent and raw material, is widely used in timber, medicine, production of resins, textile industry etc., and these industry factories arrange
The often formaldehyde containing high concentration in the waste water and gas gone out.Formaldehyde is also the main source of room air pollution, indoor first simultaneously
Aldehyde is mainly derived from construction material, furniture, artificial board, various binder coating and synthesis textile etc., is made moist in heat
In the case of, the slow release formaldehyde of meeting, the release time of some materials is even up to 3-5.
The harm of formaldehyde：Formaldehyde has strong impulse effect to the mucous membrane of eye, nose, larynx, eyes can be caused stimulated and head
Bitterly, it is serious to cause allergic dermatitis and asthma；, can be to nerve and pulmonary system if the formaldehyde of people's Long Term Contact low concentration
Cause damage；Confirmation through numerous researchers, formaldehyde also has teratogenesis carcinogenicity, can influence the normal replication of human DNA, presses down
The reparation of damage dna processed；It can also be reacted with protein, influence cell is normally metabolic.International cancer research aircraft
Structure（IARC）Formaldehyde is classified as the Ith class carcinogen.Preventing and treating to Formaldehyde Pollution of Indoor Air is a urgent problem to be solved.
The problem of currently removing the technology of indoor formaldehyde and exist：The processing method for formaldehyde mainly includes chemistry at present
Method, Physical and bioanalysis.Physical mainly uses the porous material PARA FORMALDEHYDE PRILLS(91,95) such as activated carbon to be adsorbed, but exists and inhale
The problem of attached finite capacity and temperature rise are easily desorbed.Chemical method is mainly to aoxidize, be reduced to the formaldehyde removal technology of principle,
But there is chemical reagent consumption amount height, the problem of easily causing secondary pollution.Bioanalysis is included at plant treatment method and microorganism
Logos.Certain plants such as bracketplant, aloe etc. PARA FORMALDEHYDE PRILLS(91,95) have certain absorbability, but absorption efficiency is low, and time-consuming, is only capable of
The environment relatively low for concentration of formaldehyde.Microbial degradation formaldehyde is research hot topic in recent years, because microorganism can be same by formaldehyde
Turn to own cells component or be degraded to nontoxic carbon dioxide, in the absence of secondary pollution, and using simple, thus the method by
The welcome of environmentalist is arrived.
The content of the invention
Microbial fermentation solution is concentrated except in the method for formaldehyde using microorganism for prior art, obtains having and removes first
The concentrate of aldehyde function, and most of microbe can grow at normal temperatures in concentrate, and very high is required to nutriment, make
Concentrate has that storage conditions are harsh, and the shelf-life is short, the unstable shortcoming of effect, and indoor formaldehyde is removed the invention provides one kind
Silty microbial inoculum, the silty microbial inoculum not only have microorganism remove formaldehyde general advantage, also with easy to use, long shelf-life,
Except formaldehyde efficiency high and free of contamination feature.
The present invention is also in the preparation method of the silty microbial inoculum there is provided a kind of described removal indoor formaldehyde, and this method will have
Effect microorganism is prepared into effective bacterium powder, then is mixed with needed nutrient matter, and microbial inoculum is made, i.e. available longer at normal temperatures
Shelf-life and stability.
The object of the invention is achieved through the following technical solutions.
A kind of silty microbial inoculum for removing indoor formaldehyde, by mass percentage, including following component：Effective bacterium powder 2 ~ 3%, carbon
Source 20 ~ 25%, nitrogen source 45 ~ 50%, inorganic salts 5 ~ 7%, stabilizer 20 ~ 25%.
Further, effective bacterium powder is prepared via a method which to obtain：
（1）By on the activation microbial inoculant with removal formaldehyde effect to beef extract-peptone agar medium, shaking table is vibrated
Middle culture, obtains seed liquor；
（2）Seed liquor is seeded on fermentation medium, fermented and cultured in vibration shaking table, cultivate obtained zymotic fluid carry out from
The stabilizer is added in the heart, the bacterium mud after centrifugation, vacuum freeze drying obtains effective bacterium powder.
Further, step（1）In, it is described that there is the activation microorganism for removing formaldehyde effect to include the golden gemma bars of Su Yun
One or more of secondary coccus of bacterium, Bacillus cereus, pseudomonas putida and Ye Shi.
Further, step（1）In, the culture is 12 ~ 36h of culture at 20 ~ 40 DEG C.
Further, step（2）In, the inoculum concentration is 5vol%~15vol%.
Further, step（2）In, the component of the fermentation medium is：5g/L glucose, 5g/L dusty yeasts, 5g/
L ammonium sulfate, 1g/L dipotassium hydrogen phosphates, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride.
Further, step（2）In, the fermented and cultured is to cultivate 12 ~ 36h at 20 ~ 40 DEG C.
Further, in microbial inoculum, the amount with the effective microbe for removing formaldehyde effect is 108~2×1010CFU/g。
Further, the carbon source includes one or more of starch, dextrin and glucose.
Further, the nitrogen source includes one or more of analysis for soybean powder, ammonium sulfate, peptone and dusty yeast.
Further, the inorganic salts include one or more of sylvite, magnesium salts and calcium salt.
Further, the sylvite includes dipotassium hydrogen phosphate.
Further, the magnesium salts includes magnesium sulfate.
Further, the calcium salt includes calcium chloride.
Further, the stabilizer includes skimmed milk powder.
A kind of method of the silty microbial inoculum of removal indoor formaldehyde described in any one of preparation, the quality is pressed by the component
Percentage is well mixed, and obtains the silty microbial inoculum of the removal indoor formaldehyde.
The application method of silty microbial inoculum of the present invention is：By the silty microbial inoculum of the present invention with water with mass ratio 1：50 are mixed
Close, stir evenly, stand at room temperature after 12 ~ 24h, you can be sprayed on interior, the removal for indoor formaldehyde.
Compared with prior art, the invention has the advantages that and beneficial effect：
（1）The silty microbial inoculum of the present invention has easy to use, shelf lives length, except formaldehyde efficiency high and pollution-free, effect stability
Advantage；
（2）The preparation method of silty microbial inoculum of the present invention is simple, it is easy to accomplish industrialization.
Embodiment
Below by the mode of embodiment, the invention will be further described, but the invention is not restricted to the following examples.
In the specific embodiment of the invention, the application method of silty microbial inoculum is：
By the silty microbial inoculum of preparation and water with mass ratio 1：50 are mixed, and are stirred evenly, and are stood at room temperature after 12 ~ 24h, you can sprinkling
In interior, the removal for indoor formaldehyde.
Confirmatory experiment
To verify removal effect of the silty microbial inoculum prepared to indoor formaldehyde, silty microbial inoculum prepared by embodiment is carried out as follows in fact
Test：
Formalin is sprayed in closed room, the concentration of formaldehyde in room is determined every half an hour, while using the present invention
What is prepared removes formaldehyde microbial inoculum（According to 10g/m3Amount）, microbial inoculum is diluted to liquid according to corresponding application method, carried out uniform
Sprinkling.Every after 60min, the concentration of formaldehyde is determined again.During measurement data, choose 3 representative positions and be sampled.
It can be obtained through this experiment, by 3h, the degradation rate produced by the present invention except formaldehyde microbial inoculum PARA FORMALDEHYDE PRILLS(91,95) has reached 87 ~ 98%, makes indoor first
The concentration of aldehyde is less than 0.08mg/m3, meet national standard《Code for indoor environmental pollution control of civil building engineering》.Thus may be used
, it is produced by the present invention except formaldehyde microbial inoculum have it is excellent remove formaldehyde ability, the removing of indoor formaldehyde can be widely used in.
Embodiment 1
A kind of silty microbial inoculum for removing indoor formaldehyde, including following component：
The content of effective bacterium powder is 0.02g/g, carbon source（Starch：Glucose=1：1, w/w）Content be 0.2g/g, nitrogen source（Sulfuric acid
Ammonium：Analysis for soybean powder：Peptone=1：1：1, w/w）Content be 0.5g/g, inorganic salts（Dipotassium hydrogen phosphate：Magnesium sulfate：Calcium chloride=10：
2：1, w/w）Content be 0.055g/g, the content of stabilizer skimmed milk powder is 0.245g/g, with removing having for formaldehyde effect
The content for imitating microorganism is 2 × 108CFU/g。
Effective bacterium powder is prepared via a method which：
（1）By with the activation microorganism bacillus thuringiensis for removing formaldehyde effect, Bacillus cereus, pseudomonas putida
（1：1：1, w/w）It is inoculated on beef extract-peptone agar medium（Total microbial inoculant amount is 0.1g/g）, vibrate in shaking table
37 DEG C of culture 24h, obtain seed liquor；
（2）Seed liquor is seeded in fermentation medium（Component is：5g/L glucose, 5g/L dusty yeasts, 5g/L ammonium sulfate, 1g/L
Dipotassium hydrogen phosphate, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride）On, inoculum concentration is 10vol%, in vibration shaking table, 20 DEG C of fermentation trainings
30h is supported, obtained zymotic fluid is cultivated and is centrifuged, skimmed milk powder is added in the bacterium mud after centrifugation and is used as stabilizer, vacuum refrigeration
Dry, obtain effective bacterium powder.
The preparation of the silty microbial inoculum of indoor formaldehyde is removed, step is as follows：
The component is well mixed by the mass percent, the silty microbial inoculum of the removal indoor formaldehyde is obtained.
By the silty microbial inoculum of preparation and water with mass ratio 1：50 are mixed, and are stirred evenly, and are stood after 12h, are sprayed at room temperature
After interior containing formaldehyde, 60min, it is 90.2% to measure formaldehyde clearance.
Embodiment 2
A kind of silty microbial inoculum for removing indoor formaldehyde, including following component：
The content of effective bacterium powder is 0.025g/g, carbon source（Dextrin：Glucose=1：1, w/w）Content be 0.22g/g, nitrogen source（Sulphur
Sour ammonium：Peptone：Dusty yeast=1：1：1, w/w）Content be 0.48g/g, inorganic salts（Dipotassium hydrogen phosphate：Magnesium sulfate：Calcium chloride=
10：2：1, w/w）Content be 0.05g/g, the content of stabilizer skimmed milk powder is 0.225g/g, with removing formaldehyde effect
The content of effective microbe is 6 × 108CFU/g。
Effective bacterium powder is prepared via a method which：
（1）By with the activation microorganism bacillus thuringiensis for removing formaldehyde effect, Bacillus cereus, Ye Shi pair coccuses
（1：1：1, w/w）It is inoculated on beef extract-peptone agar medium（Total microbial inoculant amount is 0.1g/g）, vibrate in shaking table
37 DEG C of culture 24h, obtain seed liquor；
（2）Seed liquor is seeded in fermentation medium（Component is：5g/L glucose, 5g/L dusty yeasts, 5g/L ammonium sulfate, 1g/L
Dipotassium hydrogen phosphate, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride）On, inoculum concentration is 15vol%, in vibration shaking table, 30 DEG C of fermentation trainings
12h is supported, obtained zymotic fluid is cultivated and is centrifuged, skimmed milk powder is added in the bacterium mud after centrifugation and is used as stabilizer, vacuum refrigeration
Dry, obtain effective bacterium powder.
The preparation of the silty microbial inoculum of indoor formaldehyde is removed, step is as follows：
The component is well mixed by the mass percent, the silty microbial inoculum of the removal indoor formaldehyde is obtained.
By the silty microbial inoculum of preparation and water with mass ratio 1：50 are mixed, and are stirred evenly, and are stood after 16h, are sprayed at room temperature
After interior containing formaldehyde, 60min, it is 88.0% to measure formaldehyde clearance.
Embodiment 3
A kind of silty microbial inoculum for removing indoor formaldehyde, including following component：
The content of effective bacterium powder is 0.028g/g, carbon source（Starch：Glucose=1：1, w/w）Content be 0.24g/g, nitrogen source（Sulphur
Sour ammonium：Analysis for soybean powder：Dusty yeast=1：1：1, w/w）Content be 0.46g/g, inorganic salts（Dipotassium hydrogen phosphate：Magnesium sulfate：Calcium chloride=
10：2：1, w/w）Content be 0.06g/g, the content of stabilizer skimmed milk powder is 0.212g/g, with removing formaldehyde effect
The content of effective microbe is 2 × 109CFU/g。
Effective bacterium powder is prepared via a method which：
（1）By with the false protein fungus of activation microorganism stench for removing formaldehyde effect, Ye Shi pair coccuses, bacillus thuringiensis
（1：1：1, w/w）It is inoculated on beef extract-peptone agar medium（Total microbial inoculant amount is 0.1g/g）, vibrate in shaking table
37 DEG C of culture 24h, obtain seed liquor；
（2）Seed liquor is seeded in fermentation medium（Component is：5g/L glucose, 5g/L dusty yeasts, 5g/L ammonium sulfate, 1g/L
Dipotassium hydrogen phosphate, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride）On, inoculum concentration is 8vol%, in vibration shaking table, 35 DEG C of fermented and cultureds
24h, cultivates obtained zymotic fluid and is centrifuged, and skimmed milk powder is added in the bacterium mud after centrifugation as stabilizer, vacuum refrigeration is done
It is dry, obtain effective bacterium powder.
The preparation of the silty microbial inoculum of indoor formaldehyde is removed, step is as follows：
The component is well mixed by the mass percent, the silty microbial inoculum of the removal indoor formaldehyde is obtained.
By the silty microbial inoculum of preparation and water with mass ratio 1：50 are mixed, and are stirred evenly, and are stood after 20h, are sprayed at room temperature
After interior containing formaldehyde, 60min, it is 94.6% to measure formaldehyde clearance.
Embodiment 4
A kind of silty microbial inoculum for removing indoor formaldehyde, including following component：
The content of effective bacterium powder is 0.03g/g, carbon source（Starch：Dextrin：Glucose=1：1：2, w/w）Content be 0.25g/g,
Nitrogen source（Ammonium sulfate：Analysis for soybean powder：Peptone：Dusty yeast=1：1：1：1, w/w）Content be 0.45g/g, inorganic salts（Phosphoric acid hydrogen two
Potassium：Magnesium sulfate：Calcium chloride=10：2：1, w/w）Content be 0.07g/g, the content of stabilizer skimmed milk powder is 0.2g/g, is had
The content for removing the effective microbe of formaldehyde effect is 2 × 1010CFU/g。
Effective bacterium powder is prepared via a method which：
（1）By with the activation microorganism pseudomonas putida for removing formaldehyde effect, Ye Shi pair coccuses, Bacillus cercus（1：
1：1, w/w）It is inoculated on beef extract-peptone agar medium（Total microbial inoculant amount is 0.1g/g）, 37 DEG C are vibrated in shaking table
24h is cultivated, seed liquor is obtained；
（2）Seed liquor is seeded in fermentation medium（Component is：5g/L glucose, 5g/L dusty yeasts, 5g/L ammonium sulfate, 1g/L
Dipotassium hydrogen phosphate, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride）On, inoculum concentration is 5vol%, in vibration shaking table, 40 DEG C of fermented and cultureds
36h, cultivates obtained zymotic fluid and is centrifuged, and skimmed milk powder is added in the bacterium mud after centrifugation as stabilizer, vacuum refrigeration is done
It is dry, obtain effective bacterium powder.
The preparation of the silty microbial inoculum of indoor formaldehyde is removed, step is as follows：
The component is well mixed by the mass percent, the silty microbial inoculum of the removal indoor formaldehyde is obtained.
By the silty microbial inoculum of preparation and water with mass ratio 1：50 are mixed, and are stirred evenly, and are stood after 24h, are sprayed at room temperature
After interior containing formaldehyde, 60 min, it is 97.9% to measure formaldehyde clearance.
Claims (10)
1. a kind of silty microbial inoculum for removing indoor formaldehyde, it is characterised in that by mass percentage, including following component：Effective bacterium
Powder 2 ~ 3%, carbon source 20 ~ 25%, nitrogen source 45 ~ 50%, inorganic salts 5 ~ 7%, stabilizer 20 ~ 25%.
2. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1, it is characterised in that effective bacterium powder is led to
Following method is crossed to prepare：
（1）By on the activation microbial inoculant with removal formaldehyde effect to beef extract-peptone agar medium, shaking table is vibrated
Middle culture, obtains seed liquor；
（2）Seed liquor is seeded on fermentation medium, fermented and cultured in vibration shaking table, cultivate obtained zymotic fluid carry out from
The stabilizer is added in the heart, the bacterium mud after centrifugation, vacuum freeze drying obtains effective bacterium powder.
3. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 2, it is characterised in that step（1）In, it is described
With remove formaldehyde effect activation microorganism include bacillus thuringiensis, Bacillus cereus, pseudomonas putida and
One or more of family name pair coccus；The culture is 12 ~ 36h of culture at 20 ~ 40 DEG C.
4. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 2, it is characterised in that step（2）In, it is described
Inoculum concentration is 5vol%~15vol%；The component of the fermentation medium is：5g/L glucose, 5g/L dusty yeasts, 5g/L sulfuric acid
Ammonium, 1g/L dipotassium hydrogen phosphates, 0.2g/L magnesium sulfate, 0.1g/L calcium chloride；The fermented and cultured be 20 ~ 40 DEG C cultivate 12 ~
36h。
5. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1, it is characterised in that in microbial inoculum, with going
Except the amount of the effective microbe of formaldehyde effect is 108~2×1010CFU/g。
6. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1, it is characterised in that the carbon source includes forming sediment
One or more of powder, dextrin and glucose.
7. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1, it is characterised in that the nitrogen source includes sulphur
One or more of sour ammonium, analysis for soybean powder, peptone and dusty yeast.
8. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1, it is characterised in that the inorganic salts include
One or more of sylvite, magnesium salts and calcium salt；The sylvite includes dipotassium hydrogen phosphate；The magnesium salts includes magnesium sulfate；The calcium
Salt includes calcium chloride.
9. a kind of silty microbial inoculum for removing indoor formaldehyde according to claim 1 or 2, it is characterised in that the stabilizer
Including skimmed milk power.
10. preparing a kind of method of the silty microbial inoculum of removal indoor formaldehyde described in any one of claim 1 ~ 9, its feature exists
In, the component is well mixed by the mass percent, obtain it is described removal indoor formaldehyde silty microbial inoculum.
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| CN114588772B (en) * | 2022-01-17 | 2022-11-25 | 北京工商大学 | Method for degrading formaldehyde |
| CN115141776A (en) * | 2022-07-21 | 2022-10-04 | 温州科技职业学院 | Method for efficiently removing formaldehyde by using composite probiotics |
| CN115141776B (en) * | 2022-07-21 | 2023-08-15 | 温州科技职业学院 | Method for efficiently removing formaldehyde by utilizing composite probiotics |
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Application publication date: 20171103 |