CN101109008A - Aquifer amendment containing multiple active microorganisms and method of preparing the same - Google Patents
Aquifer amendment containing multiple active microorganisms and method of preparing the same Download PDFInfo
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- CN101109008A CN101109008A CNA2006100290349A CN200610029034A CN101109008A CN 101109008 A CN101109008 A CN 101109008A CN A2006100290349 A CNA2006100290349 A CN A2006100290349A CN 200610029034 A CN200610029034 A CN 200610029034A CN 101109008 A CN101109008 A CN 101109008A
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Abstract
The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.
Description
Technical field
The present invention relates to a kind of various active microorganism, suitable aquifer amendment prescription of handling various water bodies such as aquaculture system, landscape water body and road, river (lake) and preparation method thereof of containing.
Background technology
Chinese patent application number 02145249.0 " microorganism cultivation aquifer amendment and making method " discloses a kind of microorganism cultivation aquifer amendment, is made up of composite bacteria, little algae, substratum, molasses, trace element solution, Semen Maydis powder, solidify material; Adopt the compound cultured method of bacterial classification, all bacterial classifications are cultivated in same substratum; Adopt composite fungus agent and little algae composite curing method; Chinese patent application number 03158365.2 " a kind of modify-water agent of viable bacteria microorganism and manufacturing process thereof of being furnished with activation medium " discloses a kind of modify-water agent of viable bacteria microorganism and manufacture method thereof of being furnished with activation medium, is used for regulating and controlling water quality and improvement that aquatic products and fancy fishes are cultured; It mainly adopts substratum is that carbon source material or nitrogen source add entry and put into fermentor tank through high-temperature sterilization, the cooling cooling, add the viable bacteria microbial inoculant, fermentation, add carrier, be positioned in the drying machine oven dry and be the solid microbe work in-process, the activation medium work in-process that form with the raw material direct packaging attach together, and become final finished product.
Find that by analysis microbiology class water body improved products mainly has the following disadvantages in the market:
1) living microorganism enters accommodative ability of environment deficiency behind the natural water in the product, survival ability in natural water is poor, successful microorganism water body improvement series products must carry out experiment sieving to the microbial strains of forming preparation, and the product of widespread use in the market is not mostly to constituting the microorganism of product, to the adaptive faculty of natural water, carried out bacterial strain screening research targetedly;
2) viable bacteria storage stability difference is the general problem that this series products exists under the normal temperature, and stability how to keep viable bacteria under the normal temperature is a technical problem that urgency is to be solved.Therefore, viable bacteria symbiosis and will propose new problem to exploitation microbiology class water body improved products in the microbiology class aquifer amendment to the problem of resistance of environment;
3) function singleness of product and effect instability are not suitable for various water bodies.
Summary of the invention
The objective of the invention is, a kind of aquifer amendment that contains the various active microorganism and preparation method thereof is provided.
A kind of preparation method who contains the aquifer amendment of various active microorganism adopts following two or more microorganism strains: Bacillus licheniformis, subtilis, bacillus megaterium, plant lactobacillus, yeast saccharomyces cerevisiae, Candida lipolytica, bacillus pumilus, bacillus cereus, swamp Rhodopseudomonas, Bacillus foecalis alkaligenes, Arthrobacter globiformis;
At first carry out the fermentation culture of bacterial strain: microorganism strains adopts the mode of liquid submerged fermentation to carry out the single strain fermentation culture, and the cultivation of thalline there is no special requirement to the nutrition source in the substratum, and the initial pH of substratum is controlled at 5～9; Culture temperature is controlled at 5～50 ℃; Incubation time is as the criterion with fermentation termination pH 3～9.
Present method can prepare liquid preparation and solid preparation.
The preparation of mixed bacteria liquid: after strain fermentation cultivate to finish, the volume percent 1～99% that accounts for mixed bacteria liquid by single bacterium zymocyte liquid was mixed and is stirred, and it is concentrated maybe will to mix back bacterium liquid; Also single bacterium zymocyte liquid can be made to concentrate volume percent 1～99% that the back account for mixed bacteria liquid by single bacterium zymocyte liquid and mix and stir, make mixed bacteria liquid; Total viable count is 10 in the quality guaranteed period
6～10
10Individual/milliliter;
The preparation of solid bacterium powder: after strain fermentation was cultivated and finished, zymocyte liquid can adopt single bacterium zymocyte liquid to mix suction type respectively with sorbent material with the adsorbents adsorb mode; Or also can make mixed bacteria liquid earlier, again with sorbent material mixing and absorption mode; Carry out drying after the absorption; Or, adding the protective material mixing again with single bacterium zymocyte liquid or the centrifugal collection thalline of mixed bacteria liquid, total viable count is 10 in the quality guaranteed period
6～10
11Individual/gram;
Sorbent material comprises following one or more, as rice chaff powder, Semen Maydis powder, wheat bran, bean cake powder, wilkinite, diatomite, attapulgite, zeolite powder etc.
Protective material comprises following one or more, as starch, dextrin, lactose, sucrose, glucose, sugar alcohol, trehalose, oligosaccharide, soybean protein, whey-protein, skim-milk, vitamins C, sodium ascorbate, Sodium Glutamate etc.
In the preparation of mixed bacteria liquid, after making mixed bacteria liquid, add the auxiliary mixing, auxiliary comprises following one or more, as phenylformic acid, Sodium Benzoate, Sorbic Acid, potassium sorbate, vitamins C, sodium ascorbate, carotene, chrysanthemum flavine, turmeric yellow, glycyrrhizic flavochrome, caramel colorant etc.; Wherein, the mass percent that mixed bacteria liquid accounts for the whole liquid preparation is 95.0～99.9%, and the mass percent that auxiliary accounts for the whole liquid preparation is 0.1～5.0%.With the can of bottling of above mixture, after packing, stick bottle and paste explanation, and after sampling inspection is qualified, be the finished fluid body preparation.Whole process of production should be carried out under certain clean environment condition, and this product normal temperature was preserved 9 months, and total viable count is 10 in the quality guaranteed period
6～10
10Individual/milliliter, and it is more stable to form the flora ratio.
Also can be in the preparation of solid bacterium powder, bacterium liquid is made solid bacterium powder, add auxiliary materials and mixing then, auxiliary material comprises following one or more, as glucose, lactose, water-soluble dietary fibre, W-Gum, rice chaff powder, Semen Maydis powder, wheat bran, bean cake powder, wilkinite, diatomite, attapulgite, zeolite powder etc.; Wherein, the mass percent that solid bacterium powder accounts for whole solid preparation is 0.01～100%, and the mass percent that auxiliary material accounts for whole solid preparation is 0～99.99%.With above hybrid dry matter dress aluminium plastic bag or Plastic Bottle,, stick bottle and paste explanation, and after sampling inspection is qualified, be the finished product solid preparation through packing.Whole process of production should be carried out under certain clean environment condition, and the humidity that controls environment is below 80%, and 30～60% to be the best, the finished product water content is controlled in 1～15%, is the best with 2～8%.This product normal temperature was preserved 18 months, and total viable count is 10 in the quality guaranteed period
6～10
11Individual/gram, and it is more stable to form the flora ratio.
Microorganism aquifer amendment of the present invention comprises liquid preparation and two kinds of formulations of solid preparation, to constituting the microorganism of product, has carried out bacterial strain screening research targetedly; The viable bacteria storage stability is good under the normal temperature; Each other symbiosis of the contained various active microorganism of the present invention and good to the tolerance of environment; Effective viable bacteria transportation and shelf in the preparation of being formed are preserved stable; Viable bacteria adaptability and viability in natural water is strong; This microorganism aquifer amendment has been gathered the suspended substance in the flocculation water body, in the water of decomposition and the organism in the bed mud, reduces COD, the BOD of water body
5, remove free nitrogen and phosphorus such as ammonia, nitrate and nitroso-group nitrogen in the water, reduce the release of ammonia, hydrogen sulfide and methane etc., suppress the growth of algae and saprophytic and pathogenic bacteria, can obviously remove stink, peculiar smell and black smelly phenomenon in the water, improve water quality and substrate, improve the transparency of water body.The present invention has multiple function, and stable effect is suitable for handling various water bodies such as aquaculture system, landscape water body and river (lake) road.
Specific embodiment
The at first screening of bacterial strain: separate, collect water body improvement microorganism strains, through Physiology and biochemistry binding molecule biology techniques, as passing through colonial morphology, pigment, gramstaining cellular form, gemma and mobility, growth temperature, pH, aerobic and nutritional status, meta-bolites and generation enzyme carbohydrate produce acid, drug susceptibility, cell-wall component, G+C/% (molar fraction), DNA-DNA hybridization, rRNA sequence, dna sequence dna etc. are identified bacterial strain.By the symbiosis analysis each other of various active microorganism, viable bacteria shelf storage stability is analyzed, and thalline enters the adaptability and the viability analysis of natural water; By water body suspended substance (SS), the COD in the water body, BOD
5, the mensuration of free nitrogen such as ammonia, nitrate and nitroso-group nitrogen and phosphorus etc.; And inhibition algae, as chlorella (Chlorella pyrenoidosa), little Nitzschia closterium minutissima (Nitaschiaclosterium minutissima), Nannochloropsis oceanica (Nannochloropsis oculata) etc. with suppress saprophytic and pathogenic bacteria, as the inhibition activation analysis of intestinal bacteria (Escherichia coli), Aeromonas hydrophila (Aeromonas hydrophila), Vibrio flurialis (Vibrio luvialis) etc.; The good bacterial classification of screening obtained performance is as Bacillus licheniformis (Bacillus licheniforms), subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), plant lactobacillus (Lactobacillus plantarum), yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), Candida lipolytica (Candida lipolytica), bacillus pumilus (Bacillus pumilus), bacillus cereus (Bacillus cereus), swamp Rhodopseudomonas (Rhodopseudomonas palustris), Bacillus foecalis alkaligenes (Alcaligenes faecalis), Arthrobacter globiformis (Arthrobacter globiformis) etc.
Embodiment 1:
A kind of aquifer amendment that contains the various active microorganism and preparation method thereof is by a good bacillus subtilis, the bacillus licheniformis of screening obtained performance;
At first carry out the fermentation culture of bacterial strain: microorganism strains adopts the liquid submerged fermentation best cultivation to carry out the single strain fermentation culture, obtains zymocyte liquid; The cultivation of thalline there is no special requirement to the nutrition source in the substratum, can use the carbon source, nitrogenous source, inorganic salt and other somatomedin that are usually used in microorganism culturing; Wherein carbon source can be glucose, sucrose, Semen Maydis powder etc., and nitrogenous source can be peptone, extractum carnis, yeast extract paste, bean cake powder etc., and inorganic salt can be potassium primary phosphate, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate etc., and somatomedin can be vitamins B
1, B
2, B
6, B
12And vitamin H etc.; The initial pH of substratum is controlled at 5～9; Culture temperature is controlled at 20～40 ℃ for good; Incubation time is as the criterion with fermentation termination pH 3～9; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter;
The preparation of mixed bacteria liquid: after strain fermentation was cultivated and finished, by volume per-cent mixed fermentation of bacillus subtilis bacterium liquid 50%, the lichen bacillus ferments bacterium liquid 50% again, obtains mixed bacteria liquid; And then in mixed bacteria liquid, add auxiliary Sodium Benzoate and yellow pigment, after mixing, obtain liquid preparation.Make liquid preparation by following prescription, by mass percentage:
Mixed bacteria liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%
With the can of bottling of above mixture, after packing, stick bottle and paste explanation, and after sampling inspection is qualified, be the finished fluid body preparation.Whole process of production should be carried out under certain clean environment condition, and this product normal temperature was preserved 9 months, and total viable count is 10 in the quality guaranteed period
6～10
10Individual/milliliter, and it is more stable to form the flora ratio.
Preparation is formed the bacterial content detection method: can adopt following one or more, measure total viable count, specificity round pcr and ERIC-PCR fingerprint image technical Analysis as the dilution plate colony counting method and form stable flora.
Embodiment 2:
A kind of preparation method who contains the aquifer amendment of various active microorganism, by screening the good microorganism strains of obtained performance: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50%, the lichen bacillus ferments bacterium liquid 50% again, obtains mixed bacteria liquid; By volume per-cent concentrates 50% with mixed bacteria liquid then, adds auxiliary Sodium Benzoate and yellow pigment again in mixed bacteria liquid, after mixing, obtains liquid preparation, and it is 10 that this liquid preparation contains total viable count
6～10
10Individual/milliliter, and it is more stable to form the flora ratio.Make liquid preparation by following prescription, by mass percentage:
Concentrate bacterium liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%
Other are with embodiment 1.
Embodiment 3:
A kind of preparation method who contains the aquifer amendment of various active microorganism, by screening the good microorganism strains of obtained performance: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50%, the lichen bacillus ferments bacterium liquid 50% again, obtains mixed bacteria liquid; Then by mass volume ratio rice chaff powder: mixed bacteria liquid is 2: 1 mixing and absorption expansion dryings, obtains solid bacterium powder; And then in solid bacterium powder, add the auxiliary material zeolite powder, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, and it is more stable to form the flora ratio.In the preparation of solid bacterium powder, ambient moisture is best 30～60%, and the finished product water content is best 2～8%.This product normal temperature was preserved 18 months.Make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 50%
Zeolite powder 50%
The fermentation culture process of bacterial strain is with embodiment 1.
Embodiment 4:
A kind of preparation method who contains the aquifer amendment of various active microorganism, by screening the good microorganism strains of obtained performance: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, again fermentation of bacillus subtilis bacterium liquid and rice chaff powder are pressed the rice chaff powder by mass volume ratio: 2: 1 mixing and absorption expansion dryings of zymocyte liquid obtain subtilis solid bacterium powder; The lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter, by mass volume ratio the lichen bacillus ferments bacterium liquid and rice chaff powder are pressed the rice chaff powder: 2: 1 mixing and absorption expansion dryings of zymocyte liquid obtain Bacillus licheniformis solid bacterium powder; Take by weighing subtilis solid bacterium powder 80%, Bacillus licheniformis solid bacterium powder 20% by mass percentage after mixing, obtain solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, and it is more stable to form the flora ratio.Make solid preparation by following prescription, by mass percentage:
Subtilis solid bacterium powder 80%
Bacillus licheniformis solid bacterium powder 20%
The fermentation culture process of bacterial strain is with embodiment 1.
Embodiment 5: a kind of preparation method who contains the aquifer amendment of various active microorganism, and by screening the good microorganism strains of obtained performance: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10It is individual/milliliter,, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50%, the lichen bacillus ferments bacterium liquid 50% again, obtains mixed bacteria liquid; Then with mixed bacterium by volume per-cent liquid concentrate 50%, press mass volume ratio rice chaff powder with the rice chaff powder again: concentrate 2: 1 mixed of bacterium liquid absorption expansion drying, acquisition solid bacterium powder; Add the auxiliary material zeolite powder again in solid bacterium powder, after mixing, obtain solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, and it is more stable to form the flora ratio.Make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 40%
Zeolite powder 60%
The fermentation culture process of bacterial strain is with embodiment 1.
Embodiment 6: a kind of preparation method who contains the aquifer amendment of various active microorganism, by screening the good microorganism strains of obtained performance: a bacillus licheniformis, a bacillus pumilus, a strain Candida lipolytica, a lactobacillus plantarum, through the single strain fermentation culture, obtain zymocyte liquid; The lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter, bacillus pumilus zymocyte liquid total viable count 10
6～10
10Individual/milliliter, Candida lipolytica zymocyte liquid total viable count 10
6～10
10Individual/milliliter, plant lactobacillus zymocyte liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes the lichen bacillus ferments bacterium liquid 40%, bacillus pumilus zymocyte liquid 20%, Candida lipolytica zymocyte liquid 20%, plant lactobacillus zymocyte liquid 20% again, obtains mixed bacteria liquid; With the centrifugal collection thalline of mixed bacteria liquid; Add 10% skim-milk and lactose then by mass percentage, after stirring, carry out lyophilize, obtain solid bacterium powder; And then in solid bacterium powder, add auxiliary material glucose, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, and it is more stable to form the flora ratio.Make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 10%
Glucose powder 90%
The fermentation culture process of bacterial strain is with embodiment 1.
Embodiment 7: a kind of preparation method who contains the aquifer amendment of various active microorganism, by screening the good microorganism strains of obtained performance: a bacillus licheniformis, a bacillus pumilus, a strain Candida lipolytica, a lactobacillus plantarum, a bacillus subtilis, a strain bacillus megaterium, a strain bacillus cereus, a strain swamp Rhodopseudomonas, a strain Bacillus foecalis alkaligenes, a strain Arthrobacter globiformis, through the single strain fermentation culture, obtain zymocyte liquid; With the centrifugal collection thalline of single strain zymocyte liquid; Add 10% skim-milk and lactose then by mass percentage, after stirring, carry out lyophilize, obtain solid bacterium powder; And then in solid bacterium powder, add auxiliary material glucose, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, and it is more stable to form the flora ratio.Make solid preparation by following prescription, by mass percentage:
Bacillus licheniformis solid bacterium powder 1%
Bacillus pumilus solid bacterium powder 1%
Candida lipolytica solid bacterium powder 1%
Plant lactobacillus solid bacterium powder 1%
Subtilis solid bacterium powder 1%
Bacillus megaterium solid bacterium powder 1%
Bacillus cereus solid bacterium powder 1%
Swamp Rhodopseudomonas solid bacterium powder 1%
Bacillus foecalis alkaligenes solid bacterium powder 1%
Arthrobacter globiformis solid bacterium powder 1%
Glucose powder 90%
The fermentation culture process of bacterial strain is with embodiment 1.
Claims (17)
1. preparation method who contains the aquifer amendment of various active microorganism, it is characterized in that, adopt following two or more microorganism strains: Bacillus licheniformis, subtilis, bacillus megaterium, plant lactobacillus, yeast saccharomyces cerevisiae, Candida lipolytica, bacillus pumilus, bacillus cereus, swamp Rhodopseudomonas, Bacillus foecalis alkaligenes, Arthrobacter globiformis;
The fermentation culture of bacterial strain: microorganism strains adopts the liquid submerged fermentation best cultivation to carry out the single strain fermentation culture, and the cultivation of thalline there is no special requirement to the nutrition source in the substratum; The initial pH of substratum is controlled at 5～9; Culture temperature is controlled at 5～50 ℃; Incubation time is as the criterion with fermentation termination pH 3～9;
The preparation of mixed bacteria liquid: after strain fermentation cultivate to finish, the volume percent 1～99% that accounts for mixed bacteria liquid by single bacterium zymocyte liquid was mixed and is stirred, and it is concentrated maybe will to mix back bacterium liquid; Or also single bacterium zymocyte liquid can be made to concentrate volume percent 1～99% that the back account for mixed bacteria liquid by single bacterium zymocyte liquid and mix and stir, make mixed bacteria liquid; Total viable count is 10 in the quality guaranteed period
6～10
10Individual/milliliter;
The preparation of solid bacterium powder: after strain fermentation was cultivated and finished, zymocyte liquid can adopt single bacterium zymocyte liquid to mix suction type respectively with sorbent material with the adsorbents adsorb mode; Or also can make mixed bacteria liquid earlier, again with sorbent material mixing and absorption mode; Carry out drying after the absorption; Or, add the protective material mixing again with single bacterium zymocyte liquid or the centrifugal collection thalline of mixed bacteria liquid, carry out drying; Total viable count is 10 in the quality guaranteed period
6～10
11Individual/gram;
Sorbent material comprises following one or more, as rice chaff powder, Semen Maydis powder, wheat bran, bean cake powder, wilkinite, diatomite, attapulgite, zeolite powder etc.;
Protective material comprises following one or more, as starch, dextrin, lactose, sucrose, glucose, sugar alcohol, trehalose, oligosaccharide, soybean protein, whey-protein, skim-milk, vitamins C, sodium ascorbate, Sodium Glutamate etc.
2. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 1, it is characterized in that in the fermentation culture of bacterial strain, the yeast culture temperature is controlled at 20～40 ℃ for good;
Drying mode: expansion drying, lyophilize;
In the preparation of solid bacterium powder, ambient moisture is best 30～60%, and the finished product water content is best 2～8%.
3. according to claim 1 or 2 described a kind of preparation methods that contain the aquifer amendment of various active microorganism, it is characterized in that, in the preparation of mixed bacteria liquid, after making mixed bacteria liquid, add the auxiliary mixing, auxiliary comprises following one or more, as phenylformic acid, Sodium Benzoate, Sorbic Acid, potassium sorbate, vitamins C, sodium ascorbate, carotene, chrysanthemum flavine, turmeric yellow, glycyrrhizic flavochrome, caramel colorant etc.; Wherein, the mass percent that mixed bacteria liquid accounts for the whole liquid preparation is 95.0～99.9%, and the mass percent that auxiliary accounts for the whole liquid preparation is 0.1～5.0%; This product normal temperature was preserved 9 months, and total viable count is 10 in the quality guaranteed period
6～10
10Individual/milliliter;
Bacterium liquid is made solid bacterium powder, add auxiliary materials and mixing then, auxiliary material comprises following one or more, as glucose, lactose, water-soluble dietary fibre, W-Gum, rice chaff powder, Semen Maydis powder, wheat bran, bean cake powder, wilkinite, diatomite, attapulgite, zeolite powder etc.; Wherein, the mass percent that solid bacterium powder accounts for whole solid preparation is 0.01～100%, and the mass percent that auxiliary material accounts for whole solid preparation is 0～99.99%; Product normal temperature was preserved 18 months, and total viable count is 10 in the quality guaranteed period
6～10
11Individual/gram.
4. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that microorganism strains: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50% again with the lichen bacillus ferments bacterium liquid 50%, obtains mixed bacteria liquid; And then in mixed bacteria liquid, add auxiliary Sodium Benzoate and yellow pigment, and after mixing, obtaining liquid preparation, it is 10 that this liquid preparation contains total viable count
6～10
10Individual/milliliter, make liquid preparation by following prescription, by mass percentage:
Mixed bacteria liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%.
5. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that microorganism strains: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50% again with the lichen bacillus ferments bacterium liquid 50%, obtains mixed bacteria liquid; By volume per-cent concentrates 50% with mixed bacteria liquid then, adds auxiliary Sodium Benzoate and yellow pigment again in mixed bacteria liquid, after mixing, obtains liquid preparation, and it is 10 that this liquid preparation contains total viable count
6～10
10Individual/milliliter, make liquid preparation by following prescription, by mass percentage:
Concentrate bacterium liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%.
6. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that microorganism strains: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50% again with the lichen bacillus ferments bacterium liquid 50%, obtains mixed bacteria liquid; Then by mass volume ratio rice chaff powder: mixed bacteria liquid is 2: 1 mixing and absorption expansion dryings, obtains solid bacterium powder; And then in solid bacterium powder, add the auxiliary material zeolite powder, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 50%
Zeolite powder 50%.
7. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that microorganism strains: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, press mass volume ratio and again rice chaff powder and fermentation of bacillus subtilis bacterium liquid are pressed 2: 1 mixing and absorption expansion dryings, obtain subtilis solid bacterium powder; The lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter, press mass volume ratio rice chaff powder and the lichen bacillus ferments bacterium liquid are pressed 2: 1 mixing and absorption expansion dryings, obtain Bacillus licheniformis solid bacterium powder; Take by weighing subtilis solid bacterium powder 80%, Bacillus licheniformis solid bacterium powder 20% by mass percentage after mixing, obtain solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, make solid preparation by following prescription, by mass percentage:
Subtilis solid bacterium powder 80%
Bacillus licheniformis solid bacterium powder 20%.
8. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that microorganism strains: a bacillus subtilis, a bacillus licheniformis through the single strain fermentation culture, obtain zymocyte liquid; Fermentation of bacillus subtilis bacterium liquid total viable count 10
6～10
10Individual/milliliter, the lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes fermentation of bacillus subtilis bacterium liquid 50% again with the lichen bacillus ferments bacterium liquid 50%, obtains mixed bacteria liquid; Then with mixed bacteria liquid by volume per-cent liquid concentrate 50%, press 2: 1 mixed of mass volume ratio absorption expansion drying with the rice chaff powder again, acquisition solid bacterium powder; Add the auxiliary material zeolite powder again in solid bacterium powder, after mixing, obtain the circle preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 40%
Zeolite powder 60%.
9. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that, microorganism strains a: bacillus licheniformis, a bacillus pumilus, a strain Candida lipolytica, a lactobacillus plantarum, through the single strain fermentation culture, obtain zymocyte liquid; The lichen bacillus ferments bacterium liquid total viable count 10
6～10
10Individual/milliliter, bacillus pumilus zymocyte liquid total viable count 10
6～10
10Individual/milliliter, Candida lipolytica zymocyte liquid total viable count 10
6～10
10Individual/milliliter, plant lactobacillus zymocyte liquid total viable count 10
6～10
10Individual/milliliter; By volume per-cent mixes the lichen bacillus ferments bacterium liquid 40%, bacillus pumilus zymocyte liquid 20%, Candida lipolytica zymocyte liquid 20%, plant lactobacillus zymocyte liquid 20% again, obtains mixed bacteria liquid; With the centrifugal collection thalline of mixed bacteria liquid; Add 10% skim-milk and lactose then by mass percentage, after stirring, carry out lyophilize, obtain solid bacterium powder; And then in solid bacterium powder, add auxiliary material glucose, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram; Make solid preparation by following prescription, by mass percentage:
Solid bacterium powder 10%
Glucose powder 90%.
10. according to the described a kind of preparation method who contains the aquifer amendment of various active microorganism of claim 3, it is characterized in that, microorganism strains a: bacillus licheniformis, a bacillus pumilus, a strain Candida lipolytica, a lactobacillus plantarum, a bacillus subtilis, a strain bacillus megaterium, a strain bacillus cereus, a strain swamp Rhodopseudomonas, a strain Bacillus foecalis alkaligenes, a strain Arthrobacter globiformis, through the single strain fermentation culture, obtain zymocyte liquid; With the centrifugal collection thalline of single strain zymocyte liquid; Add 10% skim-milk and lactose then by mass percentage, after stirring, carry out lyophilize, obtain solid bacterium powder; And then in solid bacterium powder, add auxiliary material glucose, and after mixing, obtaining solid preparation, this solid preparation contains total viable count 10
6～10
11Individual/gram, make solid preparation by following prescription, by mass percentage:
Bacillus licheniformis solid bacterium powder 1%
Bacillus pumilus solid bacterium powder 1%
Candida lipolytica solid bacterium powder 1%
Plant lactobacillus solid bacterium powder 1%
Subtilis solid bacterium powder 1%
Bacillus megaterium solid bacterium powder 1%
Bacillus cereus solid bacterium powder 1%
Swamp Rhodopseudomonas solid bacterium powder 1%
Bacillus foecalis alkaligenes solid bacterium powder 1%
Arthrobacter globiformis solid bacterium powder 1%
Glucose powder 90%.
11. the preparation method according to claim 4 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes liquid preparation by following prescription, by mass percentage:
Mixed bacteria liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%
It is 10 that this liquid preparation contains total viable count
6～10
10Individual/milliliter.
12. the preparation method according to claim 5 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes liquid preparation by following prescription, by mass percentage:
Concentrate bacterium liquid 99.8%
Sodium Benzoate 0.1%
Turmeric yellow 0.1%.
It is 10 that this liquid preparation contains total viable count
6～10
10Individual/milliliter.
13. the preparation method according to claim 6 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes solid preparation by following prescription, by mass percentage:
Solid bacterium powder 50%
Zeolite powder 50%.
This solid preparation contains total viable count 10
6～10
11Individual/.
14. the preparation method according to claim 7 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes solid preparation by following prescription, by mass percentage:
Subtilis solid bacterium powder 80%
Bacillus licheniformis solid bacterium powder 20%.
This solid preparation contains total viable count 10
6～10
11Individual/gram.
15. the preparation method according to claim 8 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes solid preparation by following prescription, by mass percentage:
Solid bacterium powder 40%
Zeolite powder 60%.
This solid preparation contains total viable count 10
6～10
11Individual/gram.
16. the preparation method according to claim 9 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes solid preparation by following prescription, by mass percentage:
Solid bacterium powder 10%
Glucose powder 90%.
This solid preparation contains total viable count 10
6～10
11Individual/gram.
17. the preparation method according to claim 10 prepares a kind of aquifer amendment that contains the various active microorganism, it is characterized in that, makes solid preparation by following prescription, by mass percentage:
Bacillus licheniformis solid bacterium powder 1%
Bacillus pumilus solid bacterium powder 1%
Candida lipolytica solid bacterium powder 1%
Plant lactobacillus solid bacterium powder 1%
Subtilis solid bacterium powder 1%
Bacillus megaterium solid bacterium powder 1%
Bacillus cereus solid bacterium powder 1%
Swamp Rhodopseudomonas solid bacterium powder 1%
Bacillus foecalis alkaligenes solid bacterium powder 1%
Arthrobacter globiformis solid bacterium powder 1%
Glucose powder 90%.
This solid preparation contains total viable count 10
6～10
11Individual/gram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100290349A CN101109008A (en) | 2006-07-18 | 2006-07-18 | Aquifer amendment containing multiple active microorganisms and method of preparing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2006100290349A CN101109008A (en) | 2006-07-18 | 2006-07-18 | Aquifer amendment containing multiple active microorganisms and method of preparing the same |
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| Publication Number | Publication Date |
|---|---|
| CN101109008A true CN101109008A (en) | 2008-01-23 |
Family
ID=39041267
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006100290349A Pending CN101109008A (en) | 2006-07-18 | 2006-07-18 | Aquifer amendment containing multiple active microorganisms and method of preparing the same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101109008A (en) |
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