CN108949739A - A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater - Google Patents
A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
Abstract
The invention belongs to microorganisms technical field, specifically a kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater.Each constituent of the complex micro organism fungicide and its weight fraction ratio are as follows: bacillus subtilis, bacillus licheniformis, bacillus megaterium, saccharomycete, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, actinomyces, nitrobacteria, denitrifying bacteria, polyP bacteria, red spirillum, thiobacillus denitrificans, Bifidobacterium, Geotrichum.Strain is immobilized into processing and obtains complex micro organism fungicide.
Description
Technical field
It is specifically a kind of for advanced treating high concentration livestock breeding wastewater the invention belongs to microorganisms technical field
Complex micro organism fungicide and preparation method thereof.
Background technique
Poultry industry is the important component of China's agricultural and rural economy, with the rapid hair of China's livestock and poultry breeding industry
Exhibition while intensive, large-scale cultivation industry emerges rapidly, also makes Pollution from livestock and poultry become the problem of can not be ignored.Scale
Change most of a large amount of fecalurias and waste water of farm's discharge without properly recycling, handling and disposing with regard to direct emission, to ring
Serious pollution is caused in border, while also directly influencing people's lives and health.The blowdown problem of many livestock and poultry cultivations
As local primary pollution source.The waste water of livestock poultry of unprocessed mistake contains the nutrients such as a large amount of organic pollutant and N, P,
Pollutional load is very high, and being discharged directly into rivers not only may cause water eutrophication, can also make quick to organic pollution materials
The aqueous bio of sense is gradually dead, or even can generate stench, causes persistent organic contamination.Waste water of livestock poultry permeates the ground for a long time
Water can make Groundwater Nitrate-nitrogen or nitrite nitrogen concentration increase, and dissolved oxygen content is reduced, and water body toxic component increases, causes
Water body deteriorates, and high concentration waste water of livestock poultry is also possible to lead to soil hardening, salinization, soil quality is seriously affected, to endanger farming
Object.
In order to reduce waste water of livestock poultry harm caused by environmental and human health impacts, waste water of livestock poultry is handled.Livestock and poultry are useless
Water treatment technology has returning to field ecologically, materializing strategy, biological treatment.(1) returning to field ecologically.Returning to field ecologically be by livestock breeding wastewater also
The method that fertilizer is made in field, this method economical and effective can not only make the pollutant in waste water of livestock poultry reach zero release of pollutant
It is required that, additionally it is possible to the nutritional ingredient in waste water is recycling in soil-plant ecosystem, reduces fertilizer amount, realizes and supports
Grow utilization of wastewater resource.(2) materializing strategy.Materializing strategy is divided into again: separation of solid and liquid, solid phase adsorption, three kinds of chemical oxidation.Gu
Liquid separation is to carry out the screen to filtrate, centrifugation, precipitating etc. to the suspended particulate of Coal Gas Washing Cycling Water, it can remove therein 40% ~
65% solid suspension reduces by 25% ~ 35% biochemical oxygen demand (BOD).Solid phase adsorption is using the solid phase with larger adsorption capacity
Material carries out adsorption treatment to waste water, and different solid-phase adsorbents can be selected according to the difference of Pollutants in Wastewater type, can
To achieve the purpose that specially to remove certain pollutant.Chemical oxidation is to generate strong oxidizing property using the higher oxidant of oxidation potential energy
Free radical, by oxygenolysis such as Organic substance in water, inorganic matters.(3) biological treatment.Biological treatment is can benefit using microorganism
Based on the various nutriments in waste water, by the way that pollutant is removed from water body the effects of absorption, conversion, metabolism, from
And the harmful substance contents in waste water drop, achieve the effect that purify water.Bioremediation is easy to operate, practical.?
In terms of biological treatment, it is difficult thoroughly to degrade the pollutant in waste water using single bacterium, and flora can combine various single
Microbial degradation ability forms a complete degeneration system, gathers a variety of enzymatic activities, expands substrate spectrum, so as to big
It is big to improve degrading activity, promote biology drop.
Chinese Patent Application No.: 201410387720.8, patent name are as follows: a kind of for the micro- of livestock breeding wastewater processing
Biological sewage inorganic agent.The breeding wastewater microbiological garbage treatment agent constituent and its weight item of the patent disclosure are as follows: 10-20 parts
Nitrobacteria, 15-30 part denitrifying bacteria, 10-50 parts of bacillus, 5-20 parts of biological enzyme, 5-15 parts of lactobacillus, 10-20
Part yeast flora, 20-40 part photosynthetic bacteria groups, containing not less than 2.0 hundred million active bacterias in monoxenic every gram of culture medium.Livestock and poultry
The characteristic of breeding wastewater selects suitable strain to optimize combination, each microorganism generated in its growth course utility and
Its secretion forms the matrix mutually grown and raw material, and forms complicated composition, a structure by mutual symbiosis, Relationship With Proliferation
Stable, function widely has the microbiologic population of varied bacterium, reciprocal mutual in the purification process of livestock breeding wastewater
Benefit plays its maximum joint advantage, has efficient treatment effect, so that discharged wastewater met the national standard, and low in cost, economy effect
It is beneficial significant.
Summary of the invention
The present invention provides a kind of complex micro organism fungicide for advanced treating high concentration livestock breeding wastewater and its preparation
Method, complex micro organism fungicide activity is high, and stability is good, can mutually symbiosis coordinate, multiple-microorganism has specific physiology
On the division of labor and cooperation, it is good to Wastewater Pollutant degradation property to playing good effect in the processing of organic wastewater with difficult degradation thereby,
Easy to use, effect is good.
The scheme of the invention is realize in this way:
A kind of complex micro organism fungicide for advanced treating high concentration livestock breeding wastewater, the complex micro organism fungicide are each
Constituent and its weight fraction ratio are as follows: 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, bacillus megaterium
8 ~ 12 parts, 25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, actinomyces 20 ~ 28
Part, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria, 12 ~ 15 parts of red spirillum, thiobacillus denitrificans 18
~ 23 parts, 15 ~ 20 parts of Bifidobacterium, 12 ~ 16 parts of Geotrichum.
As a further improvement of the present invention, the bacillus subtilis source is the feed microbial inoculum containing hay bacillus
It feeds in the excrement being discharged after duckling or chicken and is obtained by separation screening.
Bacillus subtilis acquisition methods are as follows: take fresh duckling or small chicken manure 5g be put into containing 45ml sterile water three
In the bottle of angle, a moment is shaken, this bottle is then heated to suspension boiling with small fire, maintains 15 minutes, then static 5 minutes, inhales
Take upper liquid 0.5ml to be added in the test tube of the sterile water containing 4.5ml, the suspension of 1:100 concentration be made, then by bacterium solution into
Row 10 is serially diluted again to 10-3, 10 are then drawn respectively-2With 10-3Soil suspension 0.1ml is added drop-wise to solid culture containing LB
It is coated on base plate, and culture medium is inverted in 37 DEG C constant temperature incubation 1-2 days, picked them separately from above-mentioned isolated plate
Part thallus in 2 single colonies, purifying of crossing on LB solid medium, observation and microscopy through colony characteristics, determination is bud
After spore Bacillus is purebred, picking colony obtains bacillus subtilis.Wherein, the ingredient and its system of bacillus subtilis bacterium culture medium
Standby condition are as follows: 10g tryptone, 10gNaCl, 5g yeast extract, 15g agar, 800ml sterile water, Ph7.0 ~ 7.4,121 DEG C
Sterilize 20min.
As a further improvement of the present invention, the bacillus licheniformis source is the feed containing bacillus licheniformis
Microbial inoculum is fed in the excrement being discharged after duckling or chicken and is obtained by separation screening.
Bacillus licheniformis acquisition methods are as follows: fresh duckling or small chicken manure 10g is taken to be put into the taper equipped with sterile water
In bottle, conical flask 30min is shaken, stands 1min, bacterium solution is then carried out 10 times and is serially diluted to 10-3、10-4、10-5, take respectively
10-5~10-3The dilution 0.2ml of three dilutions is coated in beef extract-peptone plate, in 37 DEG C of constant temperature incubation 48h,
The bacterium colony that picking grows from plate obtains saccharomycete.Wherein, beef-protein medium formula and its preparation condition
Are as follows: beef extract 5g, soy peptone 10g, NaCl5g, water 1L, pH value 7.0,0.1MPa sterilizing 20min.
As a further improvement of the present invention, the saccharomycete source is that the feed microbial inoculum containing saccharomycete feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Saccharomycete acquisition methods are as follows: take fresh duckling or small chicken manure 1g to be placed in 50ml enriched medium, 25 DEG C of shakes
It swings culture 2d and obtains pregnant solution, pregnant solution progress is serially diluted to 10 for 10 times-3、10-4、10-5, 10 are taken respectively-5~10-3Three
The dilution of dilution carries out painting plate in isolation medium, cultivates at 25 ~ 28 DEG C for 24 hours, and picking grows from plate
Bacterium colony obtain saccharomycete.Wherein, enrichment culture based formulas are as follows: 20g glucose, 20g peptone, 10g yeast extract, 100mg
Penicillin (excludes bacterium interference) in screening process, 1000mL distilled water；Isolation medium (PDA culture medium) formula are as follows: grape
Sugared 20g, potato 200g, agar 12g, distilled water 1000mL.
As a further improvement of the present invention, the originating in lactic acid bacterium is that the feed microbial inoculum containing lactic acid bacteria feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Lactic acid bacteria acquisition methods are as follows: take duckling or chicken fresh excreta 1g, be serially diluted again with sterile saline 10
To 10-7It is spare；Take 10-7~10-5Each 0.1ml of the dilution of three dilutions is placed in plate, is injected separately into fusing and cooling
To 50 DEG C of MRS solid medium 15ml, it is inverted plate after plate solidification, 48h is cultivated in 37 DEG C of insulating boxs, picking is longer
Colony inoculation saved into test tube slant, be then seeded into MRS fluid nutrient medium 37 DEG C and expand culture for 24 hours, grown to it
It is centrifuged at a high speed after bacterium colony, the supernatant after taking centrifugation obtains lactic acid bacteria.Wherein, MRS solid culture based formulas are as follows:
2 grams of peptone, 2 grams of beef extract, 1 gram of yeast powder, 0.4 gram of dibasic ammonium citrate, 1 gram of sodium acetate, 4 grams of glucose, Tween 80
0.2ml, 0.4 gram of dipotassium hydrogen phosphate, MgSO47H2O0.16 grams, MgSO44H2O0.25 grams, 3 grams of agar, distilled water
200ml。
As a further improvement of the present invention, the acetic acid bacteria source is that the feed microbial inoculum containing acetic acid bacteria feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Acetic acid bacteria acquisition methods are as follows: take fresh duckling or small chicken manure 10g to be added in 90ml proliferated culture medium, 30 DEG C
Then constant temperature incubation 4d respectively takes the Multiplying culture liquid of 1ml, be added in the test tube equipped with 9ml sterile saline, and concentration ladder is made
Degree is 10-1Bacteria suspension, be then gradually diluted to 10-5, 10-6, 10-7Concentration gradient draws 10-7~10-5Three concentration gradients
Each 200 μ l of dilution is inoculated on separation plate with rubbing method, and each concentration gradient is coated with 2 ~ 3 plates, 30 DEG C of constant temperature incubations
4d, wherein bacterium colony grows fine picking, and bacterium colony is evenly distributed, and transparent circle is big and clearly whole single colonies obtain acetic acid bacteria.
Wherein, proliferation culture medium formula and the preparation method comprises the following steps: yeast extract 1%, glucose 1%, 0.02% crystal violet 0.5%, Nysfungin
5mg/ml, pH5.5,0.1MPa sterilize 30min, and to be cooled to 70 DEG C whens add 3%(V/V) dehydrated alcohol；Plate isolation base
Formula and the preparation method comprises the following steps: glucose 1%, yeast extract 1%, 2%(165 DEG C of hot air sterilization 30min of calcium carbonate), dehydrated alcohol 3%, fine jade
Rouge 1.8%, pH are natural.
A method of the complex micro organism fungicide for advanced treating high concentration livestock breeding wastewater being prepared, including following
Step:
(1) take 20 ~ 28 parts of actinomyces, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria mixing equal
It is even be placed in 20 ~ 25 parts of clean soil be stirred for be uniformly mixed obtain the soil containing strain, will be done with sterile water wash
The healthy mature earthworm at 3 net monthly ages, which is placed in the soil containing strain, to be cultivated, and culturing time is to separate after 12 ~ 14 days
Out earthworm to get arrive the earthworm manure containing mixed bacteria；
(2) prepared by microbial bacterial agent carrier material: taking 20 ~ 30 parts of 200 meshes of mistake of diatomite, the salt of 5mol/L is then added to it
Acid solution is completely soaked, and 85 ~ 90min is handled under 80 ~ 85 DEG C of constant temperature, and be stirred continuously, and is filtered, repeatedly washing to neutrality, will
Filtered diatomite dries at 105 DEG C of temperature and obtains dry diatomite；2 ~ 3 parts of crystal aluminum chlorides are taken, it is dense that volume is added
Degree is in 15% sodium hydroxide solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, stand
Mixed liquor is obtained for 24 hours；Dry diatomite is added in mixed liquor, at the same be added 18 ~ 22 parts of polyvinyl alcohol, calcium alginate 3 ~
6 parts, 4 ~ 6 parts of maleic anhydride be mixed evenly after stand after 3 ~ 5h it is dry, crush and obtain particle size as 20 ~ 30 mesh
Microbial bacterial agent support powder；
(3) it takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, then be added containing mixing
The earthworm manure of strain, 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, 8 ~ 12 parts of bacillus megaterium, ferment
25 ~ 30 parts of female bacterium, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, 12 ~ 15 parts of red spirillum, denitrogenation sulphur
18 ~ 23 parts of bacillus, 15 ~ 20 parts of Bifidobacterium, 12 ~ 16 parts of Geotrichum be uniformly mixed, obtain thallus embedding liquid, finally plus
Enter 15 ~ 18 parts of polyethylene glycol, complex micro organism fungicide is arrived in 26 ~ 30 parts of condensation balling-up of chitosan to obtain the final product.
The technical principle that the present invention realizes is:
Multiple-microorganism is carried out compounding and is prepared into complex micro organism fungicide by the present invention, can be mutually total between the microbial bacterial agent
Raw collective effect handles waste water.Actinomyces, nitrobacteria, denitrifying bacteria, polyP bacteria are used for the cultivation of earthworm, contain unwrapping wire
Bacterium, nitrobacteria, denitrifying bacteria, polyP bacteria soil its surface area can increase after gizzard in earthworm body is ground
Add, favorably with the rapid conversion of microorganism fungus kind, earthworm and microbial bacterial agent interdependence in the digestion process of earthworm, mutually
Promote, not only improves earthworm and absorb nutriment, and be conducive to increasing sharply for microorganism fungus kind, pass through the digestion of earthworm
Certain variation occurs for microbial bacterial agent structure afterwards, and being capable of forming new structure of community, structure of community also compares balance surely simultaneously
It is fixed, it is adaptable, it can effectively be acted on rapidly in waste water.
Microbial bacterial agent is prepared into the activity that immobilization complex micro organism fungicide is able to maintain microorganism fungus kind, is increased micro-
Biological concentration greatly improves the treatment effeciency of the livestock breeding wastewater of high-concentration hardly-degradable, reduces processing cost.In microbial bacteria
Diatomite and polyvinyl alcohol are modified during the fixed material of agent, improve fixed effect of the diatomite to strain, poly- second
Enol is modified can to improve the problems such as its easy adhesion expands, and enhance the water-insoluble of PVA Carrier, and it is steady that it can be improved
It is qualitative, carrier gas mass-transfer performance can be enhanced after polyvinyl alcohol modification, the gas in the anti-bacterial digestion of immobilization is solved and be easy
Problem is accumulated in carrier bulk.The addition of calcium alginate is conducive to polyvinyl alcohol molecule and forms hydrogen bond in the carrier and promote
Inierpeneirating network structure formation, while calcium alginate can be spread in gel network with reaction, advanced optimize carrier structure
Be conducive to the growth of microorganism fungus kind in carrier, and then enhance substrate product and carrying intracorporal transmitting, is conducive to microorganism
Growth and the effect of degrading waste water, in addition, the gas permeability of microbial bacterial agent carrier material can be enhanced in calcium alginate.
Bacillus subtilis vitality is indomitable in microbial bacterial agent, and metabolism is vigorous, can breed and produce rapidly in water body
Raw a large amount of ectoenzyme, so as to largely consume the pollutant in waste water of livestock poultry.Bacterial digestion and anti-bacterial digestion it is common
Effect can effectively remove the ammonia nitrogen pollutant in waste water, and polyP bacteria can effectively degrade to the phosphorus substance in waste water.Cream
Sour bacterium can decompose the organic substance in waste water of livestock poultry, to be formed with the control environment conducive to culture propagation growth.Lactic acid bacteria,
The common metabolic of the strains such as saccharomycete, actinomyces, photosynthetic bacteria acts on the various substances in the waste water of livestock poultry that can effectively degrade.
The present invention has following good result:
It, can be useless to livestock and poultry 1. the present invention constructs dominant degradation bacteria agent according to the difference of strains for degrading efficiency and substrate of degrading
Water is effectively degraded.
2. the complex micro organism fungicide stability that the present invention is prepared is strong, activity is high, microbial bacterial agent can be mutually total to
Raw, collective effect, degradation efficiency are high.
3. the complex micro organism fungicide that the present invention is prepared is for handling livestock breeding wastewater, obvious processing effect, energy
The standard of livestock breeding wastewater is enough effectively reduced, reaches the relevant criterion of discharge, complex micro organism fungicide of the invention is used
In processing Wastewater from Pig Farm, wherein it is 57.61 ~ 60.34mg/L that water quality pH, which be 6.59 ~ 7.01, COD, after processing, BOD for 60.84 ~
63.54mg/L, SS be 42.69 ~ 43.67 mg/L, NH4+-N be 25.39 ~ 26.81mg/L, total phosphorus concentration be 24.52 ~
26.34mg/L high treating effect.
Specific embodiment
The description present invention is used for the composite microbial bacteria of advanced treating high concentration livestock breeding wastewater with reference to embodiments
Agent and preparation method thereof, these descriptions are not to be further limited to the content of present invention.
Embodiment 1
Take 28 parts of actinomyces, 25 parts of nitrobacteria, 28 parts of denitrifying bacteria, 21 parts of polyP bacteria be uniformly mixed be placed on clean 20
Be stirred for being uniformly mixed in part soil and obtain the soil containing strain, by with the health at 3 clean monthly ages of sterile water wash at
Ripe earthworm is placed in the soil containing strain and is cultivated, and culturing time is that earthworm is isolated after 12 days to get to containing mixing
The earthworm manure of strain；
The preparation of microbial bacterial agent carrier material: taking 20 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 85min is handled under 83 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.2 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 21 parts of polyvinyl alcohol, 4 parts of calcium alginate, maleic anhydride is added
5.5 parts be mixed evenly after stand after 3.5h it is dry, crush the microbial bacterial agent carrier powder for obtaining particle size as 22 mesh
End；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
21 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
11 parts of 23 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 27 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
25 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
From screening obtain 19.5 parts of acetic acid bacteria, 24 parts of photosynthetic bacteria, 13 parts of red spirillum, 19 parts of thiobacillus denitrificans, 16 parts of Bifidobacterium,
14 parts of Geotrichum are uniformly mixed, obtain thallus embedding liquid, be eventually adding 16 parts of polyethylene glycol, 27 parts of chitosan condense into
Ball obtains complex micro organism fungicide.
Embodiment 2
Take 20 parts of actinomyces, 22 parts of nitrobacteria, 20 parts of denitrifying bacteria, 20.5 parts of polyP bacteria be uniformly mixed be placed on it is clean
It is stirred for being uniformly mixed in 23 parts of soil and obtains the soil containing strain, by the health at 3 monthly ages with sterile water wash completely
Mature earthworm is placed in the soil containing strain and is cultivated, and culturing time is that earthworm is isolated after 13.5 days to get to containing
The earthworm manure of mixed bacteria；
The preparation of microbial bacterial agent carrier material: taking 30 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 90min is handled under 85 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；3 parts of crystal aluminum chlorides are taken, the hydroxide that volumetric concentration is 15% is added
In sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing obtains mixed liquor for 24 hours；
Dry diatomite is added in mixed liquor, while 20 parts of polyvinyl alcohol, 3 parts of calcium alginate, maleic anhydride 4.5 is added
Dry, crushing obtains the microbial bacterial agent support powder that particle size is 20 mesh part stands 4h after being mixed evenly after；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
22 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
8 parts of 24 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening are fetched derived from containing
Have the feed microbial inoculum of saccharomycete feed 25 parts of the saccharomycete obtained in the excrement being discharged after duckling or chicken by separation screening,
It fetches to feed derived from the feed microbial inoculum containing lactic acid bacteria and passes through what separation screening obtained in the excrement being discharged after duckling or chicken
30 parts of lactic acid bacteria fetches and feeds in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria by separation
Screen obtained 18 parts of acetic acid bacteria, 25 parts of photosynthetic bacteria, 14 parts of red spirillum, 23 parts of thiobacillus denitrificans, 15 parts of Bifidobacterium, Bai Di
12 parts of mould are uniformly mixed, and obtain thallus embedding liquid, are eventually adding 26 parts of 15 parts of polyethylene glycol, chitosan condensation balling-up i.e.
Obtain complex micro organism fungicide.
Embodiment 3
Take 22 parts of actinomyces, 28 parts of nitrobacteria, 30 parts of denitrifying bacteria, 22 parts of polyP bacteria be uniformly mixed be placed on clean 25
Be stirred for being uniformly mixed in part soil and obtain the soil containing strain, by with the health at 3 clean monthly ages of sterile water wash at
Ripe earthworm is placed in the soil containing strain and is cultivated, and culturing time is that earthworm is isolated after 13 days to get to containing mixing
The earthworm manure of strain；
The preparation of microbial bacterial agent carrier material: taking 22 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 86min is handled under 80 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.5 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 22 parts of polyvinyl alcohol, 6 parts of calcium alginate, maleic anhydride is added
6 parts be mixed evenly after stand after 3h it is dry, crush the microbial bacterial agent support powder for obtaining particle size as 30 mesh；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
20 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
9 parts of 25 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening are fetched derived from containing
Have the feed microbial inoculum of saccharomycete feed 26 parts of the saccharomycete obtained in the excrement being discharged after duckling or chicken by separation screening,
It fetches to feed derived from the feed microbial inoculum containing lactic acid bacteria and passes through what separation screening obtained in the excrement being discharged after duckling or chicken
28 parts of lactic acid bacteria fetches and feeds in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria by separation
Screen obtained 20 parts of acetic acid bacteria, 22 parts of photosynthetic bacteria, 12 parts of red spirillum, 18 parts of thiobacillus denitrificans, 20 parts of Bifidobacterium, Bai Di
13 parts of mould are uniformly mixed, and obtain thallus embedding liquid, are eventually adding 29 parts of 18 parts of polyethylene glycol, chitosan condensation balling-up i.e.
Obtain complex micro organism fungicide.
Embodiment 4
Take 24 parts of actinomyces, 20 parts of nitrobacteria, 22 parts of denitrifying bacteria, 21.5 parts of polyP bacteria be uniformly mixed be placed on it is clean
It is stirred for being uniformly mixed in 22 parts of soil and obtains the soil containing strain, by the health at 3 monthly ages with sterile water wash completely
Mature earthworm is placed in the soil containing strain and is cultivated, and culturing time is that earthworm is isolated after 14 days to get to containing mixed
The earthworm manure of combined bacteria kind；
The preparation of microbial bacterial agent carrier material: taking 28 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 88min is handled under 84 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2 parts of crystal aluminum chlorides are taken, the hydroxide that volumetric concentration is 15% is added
In sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing obtains mixed liquor for 24 hours；
Dry diatomite is added in mixed liquor, while 18 parts of polyvinyl alcohol, 5 parts of calcium alginate, 4 parts of maleic anhydride is added
Dry, crushing obtains the microbial bacterial agent support powder that particle size is 28 mesh after standing 4.5h after being mixed evenly；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
25 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
10 parts of 20 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 30 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
26 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
From screening obtain 18.5 parts of acetic acid bacteria, 20 parts of photosynthetic bacteria, 15 parts of red spirillum, 20 parts of thiobacillus denitrificans, 17 parts of Bifidobacterium,
16 parts of Geotrichum are uniformly mixed, obtain thallus embedding liquid, be eventually adding 17 parts of polyethylene glycol, 30 parts of chitosan condense into
Ball obtains complex micro organism fungicide.
Embodiment 5
Take 26 parts of actinomyces, 30 parts of nitrobacteria, 25 parts of denitrifying bacteria, 20 parts of polyP bacteria be uniformly mixed be placed on clean 24
Be stirred for being uniformly mixed in part soil and obtain the soil containing strain, by with the health at 3 clean monthly ages of sterile water wash at
Ripe earthworm is placed in the soil containing strain and is cultivated, and culturing time is that earthworm is isolated after 12.5 days to get to containing mixed
The earthworm manure of combined bacteria kind；
The preparation of microbial bacterial agent carrier material: taking 25 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 87min is handled under 82 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.8 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 19 parts of polyvinyl alcohol, 4 parts of calcium alginate, maleic anhydride is added
5 parts be mixed evenly after stand after 5h it is dry, crush the microbial bacterial agent support powder for obtaining particle size as 25 mesh；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
24 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
12 parts of 22 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 28 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
27 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
It is 19 parts of acetic acid bacteria being obtained from screening, 23 parts of photosynthetic bacteria, 13 parts of red spirillum, 22 parts of thiobacillus denitrificans, 18 parts of Bifidobacterium, white
15 parts of geotrichum sp are uniformly mixed, and obtain thallus embedding liquid, are eventually adding 28 parts of 16 parts of polyethylene glycol, chitosan condensation balling-up
Obtain complex micro organism fungicide.
In order to verify the application effect for the complex micro organism fungicide that the present invention is prepared, the embodiment of the present invention 1 ~ 5 is made
Standby obtained microbial bacterial agent is control for the processing of pig animal farm wastewater, while with commercially available similar complex micro organism fungicide
Group, disposition are as shown in the table.
1 complex micro organism fungicide of table handles pig animal farm wastewater situation
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, this is indicated in claim
Invention product form ingredient, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out parameter of the present invention
Range, therefore, any change within the meaning and scope equivalent to the claims of the present invention, be all considered to be including
Within the scope of the claims.
The present invention is and to pass through creative work by multidigit livestock breeding wastewater processing staff's long-term work experience accumulation
It creates and goes out, the complex micro organism fungicide activity that the present invention is prepared is high, and compatibility is good, in the waste water of livestock poultry that can effectively degrade
Pollutant reach the requirement of discharge, using simple, using effect is good, application value with higher.
Claims (2)
1. a kind of complex micro organism fungicide for advanced treating high concentration livestock breeding wastewater, characterized in that described is compound
Each constituent of microbial bacterial agent and its weight fraction ratio are as follows: 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis,
8 ~ 12 parts of bacillus megaterium, 25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria,
20 ~ 28 parts of actinomyces, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria, 12 ~ 15 parts of red spirillum,
18 ~ 23 parts of thiobacillus denitrificans, 15 ~ 20 parts of Bifidobacterium, 12 ~ 16 parts of Geotrichum；
The bacillus subtilis source is that the feed microbial inoculum containing hay bacillus feeds the excrement being discharged after duckling or chicken
Just it is obtained in by separation screening；
The bacillus licheniformis source is to be discharged after the feed microbial inoculum containing bacillus licheniformis feeds duckling or chicken
Excrement in obtained by separation screening；
The saccharomycete source is that the feed microbial inoculum containing bacillus licheniformis feeds the excrement being discharged after duckling or chicken
It is middle to be obtained by separation screening；
The originating in lactic acid bacterium is fed in the excrement being discharged after duckling or chicken for the feed microbial inoculum containing lactic acid bacteria to be passed through
Separation screening obtains；
The acetic acid bacteria source is fed in the excrement being discharged after duckling or chicken for the feed microbial inoculum containing acetic acid bacteria to be passed through
Separation screening obtains.
2. a kind of prepare the complex micro organism fungicide as described in claim 1 for being used for advanced treating high concentration livestock breeding wastewater
Method, characterized in that the following steps are included:
Step 1) takes 20 ~ 28 parts of actinomyces, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria mixing
It is uniformly placed in 20 ~ 25 parts of clean soil to be stirred for being uniformly mixed and obtains the soil containing strain, sterile water wash will be used
The healthy mature earthworm at 3 clean monthly ages, which is placed in the soil containing strain, to be cultivated, and culturing time is to divide after 12 ~ 14 days
Earthworm is separated out to get the earthworm manure containing mixed bacteria is arrived；
The preparation of step 2) microbial bacterial agent carrier material: taking 20 ~ 30 parts of 200 meshes of mistake of diatomite, and 5mol/L then is added to it
Hydrochloric acid solution be completely soaked, 85 ~ 90min is handled under 80 ~ 85 DEG C of constant temperature, and is stirred continuously, is filtered, repeatedly washing is into
Property, filtered diatomite is dried at 105 DEG C of temperature and obtains dry diatomite；2 ~ 3 parts of crystal aluminum chlorides are taken, body is added
In the sodium hydroxide solution that product concentration is 15%, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly,
Standing obtains mixed liquor for 24 hours；Dry diatomite is added in mixed liquor, while 18 ~ 22 parts of polyvinyl alcohol, alginic acid is added
3 ~ 6 parts of calcium, 4 ~ 6 parts of maleic anhydride be mixed evenly after stand after 3 ~ 5h it is dry, crush obtain particle size for 20 ~
The microbial bacterial agent support powder of 30 mesh；
Step 3) takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, and is then added containing mixed
The earthworm manure of combined bacteria kind, 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, 8 ~ 12 parts of bacillus megaterium,
25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, 12 ~ 15 parts of red spirillum, denitrogenation
18 ~ 23 parts of Thiobacillus, 15 ~ 20 parts of Bifidobacterium, 12 ~ 16 parts of Geotrichum be uniformly mixed, obtain thallus embedding liquid, finally
15 ~ 18 parts of polyethylene glycol is added, complex micro organism fungicide is arrived in 26 ~ 30 parts of condensation balling-up of chitosan to obtain the final product.
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