CN109082393A - One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof - Google Patents
One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to technical field of sewage, and specifically one kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof.The microbial bacterial agent constituent are as follows: bacillus subtilis, bacillus licheniformis, bacillus megaterium, saccharomycete, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, actinomyces, indigenous bacterium, nitrobacteria, denitrifying bacteria, polyP bacteria, red spirillum, thiobacillus denitrificans, sphingolipid bacillus, Alcaligenes faecalis, Bifidobacterium, brown rot fungus, Geotrichum, Mucor, aspergillus oryzae, heteroplasia mould.The preparation method comprises the following steps: first actinomyces, indigenous bacterium, nitrobacteria, denitrifying bacteria, polyP bacteria, Bifidobacterium are uniformly mixed and are placed in clean soil, with the soil come breeding earthworm 12 ~ 14 days, the earthworm manure containing mixed bacteria is obtained after then separating earthworm with soil；Processing is immobilized again after earthworm manure containing mixed bacteria and remaining strain are mixed obtains microbial bacterial agent.
Description
Technical field
The invention belongs to technical field of sewage, specifically one kind can be used for Treating Municipal Sewage microbial bacterial agent and
Preparation method.
Background technique
The sewage quantity for living with the fast development of urban economy, in city and producing and generating is also more and more, city
The processing problem of city's sewage is also a particularly significant problem.City domestic sewage is unable to get that effectively renovate not only can shadow
It rings and arrives The Surroundings in Cities, it is also possible to will cause the decline of city overall operation quality, especially influence city dweller's is normal
Life.Reinforce the processing to city domestic sewage, improves the treatment effect of city municipal sewage, can ensure City Life Quality
And sound development.Currently, pollutant component is also more and more in the sewage ejected with townie complexity, in addition 1 year
The variation of four seasons weather is dealt with also more and more difficult.Municipal sewage is mainly by organic matter, heavy metal inorganic matter and someization
Chemical product composition.Wherein organic matter mainly includes containing sugar substance, grease and some protein etc., and heavy metal inorganic matter mainly includes
Sodium chloride, iron ion and calcium ion etc., chemical products are mainly all kinds of nitrogenous and phosphorous chemistry that can be polluted to environment
The ingredient of product, these pollutants intersects, and brings pollution very serious for water source.
Currently, being usually used in the technology of Treating Municipal Sewage mainly has: (1) membrane separation technique.By construct corresponding film come
The polluter removed for some needs in sanitary sewage is isolated, and application foundation the most main is exactly reverse osmosis
Thoroughly, avoid some contaminant infiltrations in city domestic sewage by film, and then these polluters and city domestic sewage
Isolation, fully plays its purification of water quality purpose.The technology is in use if can cooperate agitation technology and coagulation skill
Art, and then can be improved the effect of film filtering, strengthen its purification efficiency.(2) biologic treating technique.Factually trample the result shows that, it is micro-
Biologic treating technique can have the advantage that some other physical processing techniques and chemical technology do not have, and processing doggie is compared
Obviously.Microbiological treatment technology has four classes: aerobic treatment process, anaerobic treatment process, suspension growth technique, apposition growth work
Skill.Microorganism can by sewage complicated organic matter and inorganic matter degrade, to achieve the effect that purification.(3) strengthen
Coagulation.Sewage coagulation be by simply precipitating, filtering or aeration appropriate, to remove the suspended matter in sewage,
It adjusts pH value and mitigates the technical process of the corruption degree of sewage.Processing can be by the methods of screening, gravity precipitating and flotation series connection
Composition, the particulate matter except partial size most of in decontaminated water at 100 microns or more.Sieving can remove larger substance；Gravity precipitating
The organic granular for having coherency of inorganic particle and relative density greater than 1 can be removed；Flotation can remove of the relative density less than 1
Grain object (oils etc.).Discharge standard is generally still not achieved in waste water after coagulation.Enhabced primary treatment can be in larger journey
Its whole wastewater treatment efficiency is promoted on degree, this technology is with more apparent cost of investment is few, quick, effect is brighter
The advantages such as aobvious, and can flexibly be handled, it is adaptable.
The processing method of more diversification has been formd during municipal sewage treatment in China, but it is practical in processing
Ideal effect has not been reached yet in the process.
Summary of the invention
The present invention provides a kind of microbial bacterial agent and preparation method thereof that can be used for handling city domestic sewage, micro- life
Object microbial inoculum property is stablized, and activity is high, can mutual symbiosis, common synergistic effect effectively degradation city domestic sewage, in sewage
Contaminant degradation performance it is good, easy to use, effect is good.
The scheme of the invention is realize in this way:
One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof, and the complex micro organism fungicide is respectively formed into
Point and its weight fraction ratio are as follows: 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, bacillus megaterium 8 ~ 12
Part, 25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, 20 ~ 28 parts of actinomyces, soil
It writes 18 ~ 22 parts of bacterium, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria, 12 ~ 15 parts of red spirillum, take off
18 ~ 23 parts of nitrogen Thiobacillus, 22 ~ 25 parts of sphingolipid bacillus, 15 ~ 20 parts of Alcaligenes faecalis, 15 ~ 20 parts of Bifidobacterium, brown rot fungus 10 ~ 12
Part, 12 ~ 16 parts of Geotrichum, 8 ~ 12 parts of Mucor, 12 ~ 14 parts of aspergillus oryzae, 6 ~ 10 parts of heteroplasia mould.
As a further improvement of the present invention, the bacillus subtilis source is the feed microbial inoculum containing hay bacillus
It feeds in the excrement being discharged after duckling or chicken and is obtained by separation screening.
Bacillus subtilis acquisition methods are as follows: take fresh duckling or small chicken manure 5g be put into containing 45ml sterile water three
In the bottle of angle, a moment is shaken, this bottle is then heated to suspension boiling with small fire, maintains 15 minutes, then static 5 minutes, inhales
Take upper liquid 0.5ml to be added in the test tube of the sterile water containing 4.5ml, the suspension of 1:100 concentration be made, then by bacterium solution into
Row 10 is serially diluted again to 10-3, 10 are then drawn respectively-2With 10-3Soil suspension 0.1ml is added drop-wise to solid culture containing LB
It is coated on base plate, and culture medium is inverted in 37 DEG C constant temperature incubation 1-2 days, picked them separately from above-mentioned isolated plate
Part thallus in 2 single colonies, purifying of crossing on LB solid medium, observation and microscopy through colony characteristics, determination is bud
After spore Bacillus is purebred, picking colony obtains bacillus subtilis.Wherein, the ingredient and its system of bacillus subtilis bacterium culture medium
Standby condition are as follows: 10g tryptone, 10gNaCl, 5g yeast extract, 15g agar, 800ml sterile water, Ph7.0 ~ 7.4,121 DEG C
Sterilize 20min.
As a further improvement of the present invention, the bacillus licheniformis source is the feed containing bacillus licheniformis
Microbial inoculum is fed in the excrement being discharged after duckling or chicken and is obtained by separation screening.
Bacillus licheniformis acquisition methods are as follows: fresh duckling or small chicken manure 10g is taken to be put into the taper equipped with sterile water
In bottle, conical flask 30min is shaken, stands 1min, bacterium solution is then carried out 10 times and is serially diluted to 10-3、10-4、10-5, take respectively
10-5~10-3The dilution 0.2ml of three dilutions is coated in beef extract-peptone plate, in 37 DEG C of constant temperature incubation 48h,
The bacterium colony that picking grows from plate obtains saccharomycete.Wherein, beef-protein medium formula and its preparation condition
Are as follows: beef extract 5g, soy peptone 10g, NaCl5g, water 1L, pH value 7.0,0.1MPa sterilizing 20min.
As a further improvement of the present invention, the saccharomycete source is that the feed microbial inoculum containing saccharomycete feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Saccharomycete acquisition methods are as follows: take fresh duckling or small chicken manure 1g to be placed in 50ml enriched medium, 25 DEG C of shakes
It swings culture 2d and obtains pregnant solution, pregnant solution progress is serially diluted to 10 for 10 times-3、10-4、10-5, 10 are taken respectively-5~10-3Three
The dilution of dilution carries out painting plate in isolation medium, cultivates at 25 ~ 28 DEG C for 24 hours, and picking grows from plate
Bacterium colony obtain saccharomycete.Wherein, enrichment culture based formulas are as follows: 20g glucose, 20g peptone, 10g yeast extract, 100mg
Penicillin (excludes bacterium interference) in screening process, 1000mL distilled water；Isolation medium (PDA culture medium) formula are as follows: grape
Sugared 20g, potato 200g, agar 12g, distilled water 1000mL.
As a further improvement of the present invention, the originating in lactic acid bacterium is that the feed microbial inoculum containing lactic acid bacteria feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Lactic acid bacteria acquisition methods are as follows: take duckling or chicken fresh excreta 1g, be serially diluted again with sterile saline 10
To 10-7It is spare；Take 10-7~10-5Each 0.1ml of the dilution of three dilutions is placed in plate, is injected separately into fusing and cooling
To 50 DEG C of MRS solid medium 15ml, it is inverted plate after plate solidification, 48h is cultivated in 37 DEG C of insulating boxs, picking is longer
Colony inoculation saved into test tube slant, be then seeded into MRS fluid nutrient medium 37 DEG C and expand culture for 24 hours, grown to it
It is centrifuged at a high speed after bacterium colony, the supernatant after taking centrifugation obtains lactic acid bacteria.Wherein, MRS solid culture based formulas are as follows:
2 grams of peptone, 2 grams of beef extract, 1 gram of yeast powder, 0.4 gram of dibasic ammonium citrate, 1 gram of sodium acetate, 4 grams of glucose, Tween 80
0.2ml, 0.4 gram of dipotassium hydrogen phosphate, MgSO47H2O0.16 grams, MgSO44H2O0.25 grams, 3 grams of agar, distilled water
200ml。
As a further improvement of the present invention, the acetic acid bacteria source is that the feed microbial inoculum containing acetic acid bacteria feeds duckling
It is obtained in the excrement being discharged after son or chicken by separation screening.
Acetic acid bacteria acquisition methods are as follows: take fresh duckling or small chicken manure 10g to be added in 90ml proliferated culture medium, 30 DEG C
Then constant temperature incubation 4d respectively takes the Multiplying culture liquid of 1ml, be added in the test tube equipped with 9ml sterile saline, and concentration ladder is made
Degree is 10-1Bacteria suspension, be then gradually diluted to 10-5, 10-6, 10-7Concentration gradient draws 10-7~10-5Three concentration gradients
Each 200 μ l of dilution is inoculated on separation plate with rubbing method, and each concentration gradient is coated with 2 ~ 3 plates, 30 DEG C of constant temperature incubations
4d, wherein bacterium colony grows fine picking, and bacterium colony is evenly distributed, and transparent circle is big and clearly whole single colonies obtain acetic acid bacteria.
Wherein, proliferation culture medium formula and the preparation method comprises the following steps: yeast extract 1%, glucose 1%, 0.02% crystal violet 0.5%, Nysfungin
5mg/ml, pH5.5,0.1MPa sterilize 30min, and to be cooled to 70 DEG C whens add 3%(V/V) dehydrated alcohol；Plate isolation base
Formula and the preparation method comprises the following steps: glucose 1%, yeast extract 1%, 2%(165 DEG C of hot air sterilization 30min of calcium carbonate), dehydrated alcohol 3%, fine jade
Rouge 1.8%, pH are natural.
A kind of prepare can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof, comprising the following steps:
(1) 20 ~ 28 parts of actinomyces, indigenous 18 ~ 22 parts of bacterium, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, polyP bacteria are taken
20 ~ 22 parts, 15 ~ 20 parts of Bifidobacterium be uniformly mixed are placed in 20 ~ 25 parts of clean soil be stirred for be uniformly mixed contained
Have the soil of strain, by with the healthy mature earthworm at 3 clean monthly ages of sterile water wash be placed in the soil containing strain into
Row cultivation, culturing time are that earthworm is isolated after 12 ~ 14 days to get the earthworm manure containing mixed bacteria is arrived；
(2) prepared by microbial bacterial agent carrier material: taking 20 ~ 30 parts of 200 meshes of mistake of diatomite, the salt of 5mol/L is then added to it
Acid solution is completely soaked, and 85 ~ 90min is handled under 80 ~ 85 DEG C of constant temperature, and be stirred continuously, and is filtered, repeatedly washing to neutrality, will
Filtered diatomite dries at 105 DEG C of temperature and obtains dry diatomite；2 ~ 3 parts of crystal aluminum chlorides are taken, it is dense that volume is added
Degree is in 15% sodium hydroxide solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, stand
Mixed liquor is obtained for 24 hours；Dry diatomite is added in mixed liquor, at the same be added 18 ~ 22 parts of polyvinyl alcohol, calcium alginate 3 ~
6 parts, 4 ~ 6 parts of maleic anhydride be mixed evenly after stand after 3 ~ 5h it is dry, crush and obtain particle size as 20 ~ 30 mesh
Microbial bacterial agent support powder；
(3) it takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, then be added containing mixing
The earthworm manure of strain, 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, 8 ~ 12 parts of bacillus megaterium, ferment
25 ~ 30 parts of female bacterium, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, 12 ~ 15 parts of red spirillum, denitrogenation sulphur
18 ~ 23 parts of bacillus, 22 ~ 25 parts of sphingolipid bacillus, 15 ~ 20 parts of Alcaligenes faecalis, 10 ~ 12 parts of brown rot fungus, Geotrichum 12 ~ 16 part, mao
Mould 8 ~ 12 parts, 12 ~ 14 parts of aspergillus oryzae, 6 ~ 10 parts of heteroplasia mould be uniformly mixed, obtain thallus embedding liquid, be eventually adding poly- second
Microbial bacterial agent is arrived in 30 ~ 35 parts of 20 ~ 25 parts of glycol, chitosan condensation balling-up to obtain the final product.
The technical principle that the present invention realizes is:
Multiple-microorganism is carried out compounding and is prepared into complex micro organism fungicide by the present invention, can be mutually total between the microbial bacterial agent
Raw collective effect handles sanitary sewage.Actinomyces, nitrobacteria, denitrifying bacteria, polyP bacteria, Bifidobacterium are used for earthworm
Cultivation, the soil containing actinomyces, nitrobacteria, denitrifying bacteria, polyP bacteria, Bifidobacterium can pass through gizzard in earthworm body
Its surface area greatly increases after grinding, favorably with the rapid conversion of microorganism fungus kind, the earthworm and micro- in the digestion process of earthworm
Bacteria agent interdependence, mutually promotes, and not only improves earthworm and absorbs nutriment, and is conducive to the rapid increasing of microorganism fungus kind
Add, certain variation is occurred by microbial bacterial agent structure after the digestion of earthworm, is capable of forming new structure of community simultaneously
Structure of community also compares balance and stability, adaptable, can effectively act on rapidly in sanitary sewage.
Microbial bacterial agent is prepared into the activity that immobilization complex micro organism fungicide is able to maintain microorganism fungus kind, is increased micro-
Biological concentration greatly improves the treatment effeciency of the sanitary sewage of high-concentration hardly-degradable, reduces processing cost.It is solid in microbial bacterial agent
Determine to be modified diatomite and polyvinyl alcohol during material, improves fixed effect of the diatomite to strain, polyvinyl alcohol
Modified the problems such as can improving its easy adhesion expansion, enhance the water-insoluble of PVA Carrier, and its stability can be improved,
Carrier gas mass-transfer performance can be enhanced after polyvinyl alcohol modification, the gas in the anti-bacterial digestion of immobilization is solved and be easy in carrier
Accumulation problem in volume.The addition of calcium alginate is conducive to polyvinyl alcohol molecule and forms hydrogen bond in the carrier and promote interpenetrating net
The formation of network structure, while calcium alginate can be spread in gel network with reaction, advanced optimized carrier structure and be conducive to
The growth of microorganism fungus kind in carrier, and then enhance substrate product and carrying intracorporal transmitting, be conducive to the growth of microorganism with
And the degradation to sewage, in addition, the gas permeability of microbial bacterial agent carrier material can be enhanced in calcium alginate.
Bacillus subtilis vitality is indomitable in microbial bacterial agent, and metabolism is vigorous, can breed and produce rapidly in water body
Raw a large amount of ectoenzyme, so as to largely consume the pollutant in sanitary sewage.Bacterial digestion and anti-bacterial digestion it is common
Effect can effectively remove the ammonia nitrogen pollutant in sanitary sewage, and bacillus megaterium and polyP bacteria can be to the phosphorus objects in sewage
Matter is effectively degraded.Lactic acid bacteria can decompose the organic substance in sanitary sewage, to be formed with conducive to culture propagation growth
Control environment.Sheath phosphorus bacillus can degrade to the lipid in sanitary sewage, and Alcaligenes faecalis can be decomposed effectively in sewage
Organic matter, eliminate water in peculiar smell.The common metabolics of the strains such as lactic acid bacteria, saccharomycete, actinomyces, photosynthetic bacteria act on and
The synergistic effect of the bacterium such as Mucor, aspergillus oryzae, heteroplasia mould can effectively degrade the various pollutants matter in sanitary sewage, reach it
To the requirement of discharge.
The present invention has following good result:
1. the present invention is constructed according to the difference of strains for degrading efficiency and substrate of degrading and is handled city domestic sewage
Dominant degradation bacteria agent can effectively degrade to city domestic sewage.
2. microbial bacterial agent stability that the present invention is prepared is strong, activity is high, microbial bacterial agent being capable of mutually symbiosis, altogether
Same-action, degradation efficiency are high.
3. the microbial bacterial agent that the present invention is prepared is for handling city domestic sewage, obvious processing effect, Neng Gouyou
Effect reduces the standard of city domestic sewage, reaches the relevant criterion of discharge, microbial bacterial agent of the invention is for handling city
City's sanitary sewage, wherein it is 10.13 ~ 11.07mg/L that water quality pH, which be 6.58 ~ 7.26, COD, after processing, BOD for 10.98 ~
12.36mg/L, SS are 5.76 ~ 6.33mg/L, and ammonia nitrogen is 0.09 ~ 0.14mg/L, and total nitrogen is 11.64 ~ 12.36mg/L, and total phosphorus is
0.41 ~ 0.49mg/L, high treating effect, treated water quality odorless.
Specific embodiment
With reference to embodiments a kind of description microbial bacterial agent that can be used for handling city domestic sewage of the present invention and its
Preparation method, these descriptions are not to be further limited to the content of present invention.
Embodiment 1
Take 28 parts of actinomyces, indigenous 22 parts of bacterium, 25 parts of nitrobacteria, 28 parts of denitrifying bacteria, 21 parts of polyP bacteria, Bifidobacterium 16
Part, be uniformly mixed be placed in 20 parts of clean soil be stirred for be uniformly mixed obtain the soil containing strain, sterile water will be used
The healthy mature earthworm at 3 monthly ages cleaned up, which is placed in the soil containing strain, to be cultivated, and culturing time is after 12 days
Earthworm is isolated to get the earthworm manure containing mixed bacteria is arrived；
The preparation of microbial bacterial agent carrier material: taking 20 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 85min is handled under 83 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.2 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 21 parts of polyvinyl alcohol, 4 parts of calcium alginate, maleic anhydride is added
5.5 parts be mixed evenly after stand after 3.5h it is dry, crush the microbial bacterial agent carrier powder for obtaining particle size as 22 mesh
End；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
21 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
11 parts of 23 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 27 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
25 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
From screening obtain 19.5 parts of acetic acid bacteria, 24 parts of photosynthetic bacteria, 13 parts of red spirillum, 19 parts of thiobacillus denitrificans, 23 parts of sphingolipid bacillus,
17 parts of Alcaligenes faecalis, 10 parts of brown rot fungus, 14 parts of Geotrichum, 11 parts of Mucor, 13.5 parts of aspergillus oryzae, 9 parts of heteroplasia mould stirrings are mixed
It closes uniformly, obtains thallus embedding liquid, be eventually adding 21 parts of polyethylene glycol, microbial bacteria is arrived in 30 parts of condensation balling-up of chitosan to obtain the final product
Agent.
Embodiment 2
Take 20 parts of actinomyces, indigenous 18 parts of bacterium, 22 parts of nitrobacteria, 20 parts of denitrifying bacteria, 20.5 parts of polyP bacteria, Bifidobacterium
15 parts, be uniformly mixed to be placed in 23 parts of clean soil to be stirred for being uniformly mixed and obtain the soil containing strain, will be with sterile
The healthy mature earthworm at 3 monthly ages that water cleans up, which is placed in the soil containing strain, to be cultivated, culturing time 13.5
Earthworm is isolated after it to get the earthworm manure containing mixed bacteria is arrived；
The preparation of microbial bacterial agent carrier material: taking 30 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 90min is handled under 85 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；3 parts of crystal aluminum chlorides are taken, the hydroxide that volumetric concentration is 15% is added
In sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing obtains mixed liquor for 24 hours；
Dry diatomite is added in mixed liquor, while 20 parts of polyvinyl alcohol, 3 parts of calcium alginate, maleic anhydride 4.5 is added
Dry, crushing obtains the microbial bacterial agent support powder that particle size is 20 mesh part stands 4h after being mixed evenly after；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
22 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
8 parts of 24 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening are fetched derived from containing
Have the feed microbial inoculum of saccharomycete feed 25 parts of the saccharomycete obtained in the excrement being discharged after duckling or chicken by separation screening,
It fetches to feed derived from the feed microbial inoculum containing lactic acid bacteria and passes through what separation screening obtained in the excrement being discharged after duckling or chicken
30 parts of lactic acid bacteria fetches and feeds in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria by separation
18 parts obtained of acetic acid bacteria, 25 parts of photosynthetic bacteria, 14 parts of red spirillum, 23 parts of thiobacillus denitrificans, 24 parts of sphingolipid bacillus, excrement is screened to produce
15 parts of alkali bacterium, 12 parts of brown rot fungus, 12 parts of Geotrichum, 9 parts of Mucor, 13 parts of aspergillus oryzae, 8 parts of heteroplasia mould be uniformly mixed,
Thallus embedding liquid is obtained, is eventually adding 25 parts of polyethylene glycol, microbial bacterial agent is arrived in 33 parts of condensation balling-up of chitosan to obtain the final product.
Embodiment 3
Take 22 parts of actinomyces, indigenous 19 parts of bacterium, 28 parts of nitrobacteria, 30 parts of denitrifying bacteria, 22 parts of polyP bacteria, Bifidobacterium 20
Part, be uniformly mixed be placed in 25 parts of clean soil be stirred for be uniformly mixed obtain the soil containing strain, sterile water will be used
The healthy mature earthworm at 3 monthly ages cleaned up, which is placed in the soil containing strain, to be cultivated, and culturing time is after 13 days
Earthworm is isolated to get the earthworm manure containing mixed bacteria is arrived；
The preparation of microbial bacterial agent carrier material: taking 22 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 86min is handled under 80 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.5 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 22 parts of polyvinyl alcohol, 6 parts of calcium alginate, maleic anhydride is added
6 parts be mixed evenly after stand after 3h it is dry, crush the microbial bacterial agent support powder for obtaining particle size as 30 mesh；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
20 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
9 parts of 25 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening are fetched derived from containing
Have the feed microbial inoculum of saccharomycete feed 26 parts of the saccharomycete obtained in the excrement being discharged after duckling or chicken by separation screening,
It fetches to feed derived from the feed microbial inoculum containing lactic acid bacteria and passes through what separation screening obtained in the excrement being discharged after duckling or chicken
28 parts of lactic acid bacteria fetches and feeds in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria by separation
20 parts obtained of acetic acid bacteria, 22 parts of photosynthetic bacteria, 12 parts of red spirillum, 18 parts of thiobacillus denitrificans, 25 parts of sphingolipid bacillus, excrement is screened to produce
16 parts of alkali bacterium, 11.5 parts of brown rot fungus, 13 parts of Geotrichum, 12 parts of Mucor, 12.5 parts of aspergillus oryzae, 10 parts of heteroplasia mould be stirred
Uniformly, thallus embedding liquid is obtained, is eventually adding 24 parts of polyethylene glycol, microbial bacterial agent is arrived in 35 parts of condensation balling-up of chitosan to obtain the final product.
Embodiment 4
Take 24 parts of actinomyces, indigenous 21 parts of bacterium, 20 parts of nitrobacteria, 22 parts of denitrifying bacteria, 21.5 parts of polyP bacteria, Bifidobacterium
17 parts, be uniformly mixed to be placed in 22 parts of clean soil to be stirred for being uniformly mixed and obtain the soil containing strain, will be with sterile
The healthy mature earthworm at 3 monthly ages that water cleans up, which is placed in the soil containing strain, to be cultivated, and culturing time is 14 days
Isolate earthworm afterwards to get the earthworm manure containing mixed bacteria is arrived；
The preparation of microbial bacterial agent carrier material: taking 28 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 88min is handled under 84 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2 parts of crystal aluminum chlorides are taken, the hydroxide that volumetric concentration is 15% is added
In sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing obtains mixed liquor for 24 hours；
Dry diatomite is added in mixed liquor, while 18 parts of polyvinyl alcohol, 5 parts of calcium alginate, 4 parts of maleic anhydride is added
Dry, crushing obtains the microbial bacterial agent support powder that particle size is 28 mesh after standing 4.5h after being mixed evenly；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
25 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
10 parts of 20 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 30 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
26 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
From screening obtain 18.5 parts of acetic acid bacteria, 20 parts of photosynthetic bacteria, 15 parts of red spirillum, 20 parts of thiobacillus denitrificans, 22 parts of sphingolipid bacillus,
20 parts of Alcaligenes faecalis, 11 parts of brown rot fungus, 16 parts of Geotrichum, 10 parts of Mucor, 12 parts of aspergillus oryzae, 7 parts of heteroplasia mould be stirred
Uniformly, thallus embedding liquid is obtained, is eventually adding 20 parts of polyethylene glycol, microbial bacterial agent is arrived in 32 parts of condensation balling-up of chitosan to obtain the final product.
Embodiment 5
Take 26 parts of actinomyces, indigenous 20 parts of bacterium, 30 parts of nitrobacteria, 25 parts of denitrifying bacteria, 20 parts of polyP bacteria, Bifidobacterium 18
Part, be uniformly mixed be placed in 24 parts of clean soil be stirred for be uniformly mixed obtain the soil containing strain, sterile water will be used
The healthy mature earthworm at 3 monthly ages cleaned up, which is placed in the soil containing strain, to be cultivated, and culturing time is 12.5 days
Isolate earthworm afterwards to get the earthworm manure containing mixed bacteria is arrived；
The preparation of microbial bacterial agent carrier material: taking 25 parts of 200 meshes of mistake of diatomite, and the hydrochloric acid solution of 5mol/L is then added to it
It is completely soaked, 87min is handled under 82 DEG C of constant temperature, and be stirred continuously, filter, repeatedly washing is to neutrality, by filtered diatom
Soil dries at 105 DEG C of temperature and obtains dry diatomite；2.8 parts of crystal aluminum chlorides are taken, the hydrogen-oxygen that volumetric concentration is 15% is added
Change in sodium solution, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly, and standing is mixed for 24 hours
Liquid；Dry diatomite is added in mixed liquor, while 19 parts of polyvinyl alcohol, 4 parts of calcium alginate, maleic anhydride is added
5 parts be mixed evenly after stand after 5h it is dry, crush the microbial bacterial agent support powder for obtaining particle size as 25 mesh；
It takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, is then added and contains mixed bacteria
Earthworm manure, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing hay bacillus through excessive
24 parts of bacillus subtilis obtaining from screening fetches and feeds duckling or small derived from the feed microbial inoculum containing bacillus licheniformis
12 parts of 22 parts of bacillus licheniformis, bacillus megaterium obtaining in the excrement being discharged after chicken by separation screening fetch and are derived from
Feed microbial inoculum containing saccharomycete feeds the saccharomycete 28 obtained in the excrement being discharged after duckling or chicken by separation screening
Part, fetch to feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing lactic acid bacteria and obtained by separation screening
27 parts of lactic acid bacteria, fetch and feed in the excrement being discharged after duckling or chicken derived from the feed microbial inoculum containing acetic acid bacteria through excessive
19 parts of acetic acid bacteria, 23 parts of photosynthetic bacteria, 13 parts of red spirillum, 22 parts of the thiobacillus denitrificans, 23 parts of sphingolipid bacillus, excrement obtained from screening
18 parts of Alcaligenes, 10.5 parts of brown rot fungus, 15 parts of Geotrichum, 8 parts of Mucor, 14 parts of aspergillus oryzae, 6 parts of heteroplasia mould be stirred
It is even, thallus embedding liquid is obtained, is eventually adding 23 parts of polyethylene glycol, microbial bacterial agent is arrived in 34 parts of condensation balling-up of chitosan to obtain the final product.
In order to verify the practical application effect for the microbial bacterial agent that the present invention is prepared, the embodiment of the present invention 1 ~ 5 is made
Standby obtained microbial bacterial agent is used for the processing of city domestic sewage, while using commercially available same organism microbial inoculum as control group,
Disposition is as shown in the table.
1 complex micro organism fungicide of table handles city domestic sewage situation
The above embodiment of the present invention scheme is only the description of the invention and cannot limit the present invention, this is indicated in claim
Invention product form ingredient, component ratio, the range of preparation method parameter, and above-mentioned explanation does not point out parameter of the present invention
Range, therefore, any change within the meaning and scope equivalent to the claims of the present invention, be all considered to be including
Within the scope of the claims.
The present invention is and to pass through creative work by multidigit sanitary sewage disposal technical staff's long-term work experience accumulation
It creates and goes out, the microbial bacterial agent activity that the present invention is prepared is high, stability is strong, the dirt in the sanitary sewage that can effectively degrade
Contaminate object, easy to use, good degrading effect, application value height.
Claims (2)
1. one kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof, characterized in that the complex microorganism
Each constituent of microbial inoculum and its weight fraction ratio are as follows: 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, huge bud
8 ~ 12 parts of spore bacillus, 25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, actinomyces
20 ~ 28 parts, indigenous 18 ~ 22 parts of bacterium, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, 20 ~ 22 parts of polyP bacteria, red spirillum
12 ~ 15 parts, it is 18 ~ 23 parts of thiobacillus denitrificans, 22 ~ 25 parts of sphingolipid bacillus, 15 ~ 20 parts of Alcaligenes faecalis, 15 ~ 20 parts of Bifidobacterium, brown
10 ~ 12 parts of rotten bacterium, 12 ~ 16 parts of Geotrichum, 8 ~ 12 parts of Mucor, 12 ~ 14 parts of aspergillus oryzae, 6 ~ 10 parts of heteroplasia mould；
The bacillus subtilis source is that the feed microbial inoculum containing hay bacillus feeds the excrement being discharged after duckling or chicken
Just it is obtained in by separation screening；
The bacillus licheniformis source is to be discharged after the feed microbial inoculum containing bacillus licheniformis feeds duckling or chicken
Excrement in obtained by separation screening；
The saccharomycete source is that the feed microbial inoculum containing bacillus licheniformis feeds the excrement being discharged after duckling or chicken
It is middle to be obtained by separation screening；
The originating in lactic acid bacterium is fed in the excrement being discharged after duckling or chicken for the feed microbial inoculum containing lactic acid bacteria to be passed through
Separation screening obtains；
The acetic acid bacteria source is fed in the excrement being discharged after duckling or chicken for the feed microbial inoculum containing acetic acid bacteria to be passed through
Separation screening obtains.
2. a kind of prepare can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof as described in claim 1, special
Sign is, comprising the following steps:
Step 1) takes 20 ~ 28 parts of actinomyces, indigenous 18 ~ 22 parts of bacterium, 20 ~ 30 parts of nitrobacteria, 20 ~ 30 parts of denitrifying bacteria, gathers
20 ~ 22 parts of phosphorus bacterium, 15 ~ 20 parts of Bifidobacterium be uniformly mixed be placed in 20 ~ 25 parts of clean soil be stirred for it is uniformly mixed
To the soil containing strain, the soil containing strain will be placed in the healthy mature earthworm at 3 clean monthly ages of sterile water wash
In cultivated, culturing time is that earthworm is isolated after 12 ~ 14 days to get to the earthworm manure containing mixed bacteria；
The preparation of step 2) microbial bacterial agent carrier material: taking 20 ~ 30 parts of 200 meshes of mistake of diatomite, and 5mol/L then is added to it
Hydrochloric acid solution be completely soaked, 85 ~ 90min is handled under 80 ~ 85 DEG C of constant temperature, and is stirred continuously, is filtered, repeatedly washing is into
Property, filtered diatomite is dried at 105 DEG C of temperature and obtains dry diatomite；2 ~ 3 parts of crystal aluminum chlorides are taken, body is added
In the sodium hydroxide solution that product concentration is 15%, so that aluminium chloride and the final molar ratio of aluminium hydroxide are 2: 1, stirring is allowed to uniformly,
Standing obtains mixed liquor for 24 hours；Dry diatomite is added in mixed liquor, while 18 ~ 22 parts of polyvinyl alcohol, alginic acid is added
3 ~ 6 parts of calcium, 4 ~ 6 parts of maleic anhydride be mixed evenly after stand after 3 ~ 5h it is dry, crush obtain particle size for 20 ~
The microbial bacterial agent support powder of 30 mesh；
Step 3) takes the microbial bacterial agent support powder being prepared to be added in sterile water to be dissolved, and is then added containing mixed
The earthworm manure of combined bacteria kind, 20 ~ 25 parts of bacillus subtilis, 20 ~ 25 parts of bacillus licheniformis, 8 ~ 12 parts of bacillus megaterium,
25 ~ 30 parts of saccharomycete, 25 ~ 30 parts of lactic acid bacteria, 18 ~ 20 parts of acetic acid bacteria, 20 ~ 25 parts of photosynthetic bacteria, 12 ~ 15 parts of red spirillum, denitrogenation
18 ~ 23 parts of Thiobacillus, 22 ~ 25 parts of sphingolipid bacillus, 15 ~ 20 parts of Alcaligenes faecalis, 10 ~ 12 parts of brown rot fungus, 12 ~ 16 parts of Geotrichum,
8 ~ 12 parts of Mucor, 12 ~ 14 parts of aspergillus oryzae, 6 ~ 10 parts of heteroplasia mould be uniformly mixed, obtain thallus embedding liquid, be eventually adding poly-
Microbial bacterial agent is arrived in 30 ~ 35 parts of 20 ~ 25 parts of ethylene glycol, chitosan condensation balling-up to obtain the final product.
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| CN109626601A (en) * | 2019-02-20 | 2019-04-16 | 何美英 | A kind of complex microorganism water purification agent and its preparation method and application |
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Application publication date: 20181225 |