CN111233167A - Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation - Google Patents
Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation Download PDFInfo
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- CN111233167A CN111233167A CN202010069156.0A CN202010069156A CN111233167A CN 111233167 A CN111233167 A CN 111233167A CN 202010069156 A CN202010069156 A CN 202010069156A CN 111233167 A CN111233167 A CN 111233167A
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- biogas slurry
- alcaligenes faecalis
- chicken manure
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- 229940005347 alcaligenes faecalis Drugs 0.000 title claims abstract description 61
- 238000005189 flocculation Methods 0.000 title claims abstract description 60
- 230000016615 flocculation Effects 0.000 title claims abstract description 60
- 210000003608 fece Anatomy 0.000 title claims abstract description 53
- 239000010871 livestock manure Substances 0.000 title claims abstract description 47
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 45
- 238000000855 fermentation Methods 0.000 claims abstract description 53
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- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 17
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
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- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
- -1 aluminum ions Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
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- 231100000568 intoxicate Toxicity 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
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- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention belongs to the field of microorganisms, and particularly relates to an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation. The concrete application is as follows: adding the alcaligenes faecalis fermentation product into the chicken manure biogas slurry, adding the coagulant aid, uniformly mixing, and standing. By using the method, the chicken manure biogas slurry can be specifically flocculated, and the flocculation effect is up to more than 90%.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation.
Background
In recent years, with the continuous expansion of livestock and poultry breeding scale, the breeding sewage discharge amount is increased day by day. If the culture wastewater is directly discharged into the environment, serious environmental problems are caused. Anaerobic digestion is a common method for treating breeding sewage, can realize harmless treatment of livestock and poultry breeding wastes, and can also generate biogas and provide clean energy. Meanwhile, along with the rapid development of biogas engineering, a large amount of biogas slurry is generated. But the biogas slurry has the defects of high water content, difficult storage and transportation, relatively low economic value and the like. When the engineering scale is too large and the produced biogas slurry amount far exceeds the maximum consumption of the local farmland, the biogas slurry becomes wastewater which is difficult to treat. If the part of biogas slurry is discharged randomly, the natural environment, especially the water body environment, can be seriously damaged. Therefore, it is important to perform harmless treatment on the biogas slurry to make the biogas slurry reach relevant standards. However, the existing treatment methods for biogas slurry all require flocculation and precipitation to remove particle suspended matters (SS) in the biogas slurry.
The flocculating agents widely used at present mainly comprise: inorganic polymeric flocculants typified by aluminum, and organic polymeric flocculants typified by polyacrylamide. The inorganic flocculant has the advantages of economy and simple use method; but also has the defects of large dosage, low flocculation effect, high cost, strong corrosivity and the like. The organic polymer flocculant is a novel wastewater treatment agent developed in the later stage of the 60 th century, and compared with the traditional flocculant, the organic polymer flocculant can improve the efficiency by times, has small using amount, low scum yield, strong flocculation capacity, easy separation of flocs and good oil removal and suspended matter removal effects, and is suitable for the treatment of wastewater in the later stage of the 20 th century. However, most organic polymeric flocculants are toxic or hydrolysis and degradation products thereof, and the acrylamide monomers used for synthesis are toxic, so that the organic polymeric flocculants can intoxicate the central nerve of people, and residual monomers have 'triple-effect' (teratogenicity, carcinogenicity and mutagenicity), thereby being not in line with the current trend of environment-friendly development. In addition, when inorganic polymers or organic polymers are used as the flocculant, the presence of a large amount of aluminum ions or polyacrylamide in the precipitate may also affect the use of the biogas residues or biogas slurry as a high-quality fertilizer.
Because the microbial flocculant can overcome the inherent defects of inorganic polymer and synthetic organic polymer flocculants, has no secondary pollution and safe use, the research on the microbial flocculant is increasingly emphasized in recent years. However, the biogas slurry has a certain sterilization effect, and microbial strains which can grow well in the biogas slurry are deficient in resources, so that the flocculation effect is not ideal. In addition, due to different components of different biogas slurries, a strain which can grow well in one biogas slurry does not necessarily adapt to the environment of another biogas slurry. Therefore, microbial flocculants applicable to biogas slurry have been limited in development. If the microbial flocculant with a specific flocculation effect can be provided for specific biogas slurry, the method has important practical significance.
Disclosure of Invention
The invention aims to provide an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: an alcaligenes faecalis preserved in the China general microbiological culture Collection center in 2019, 5 months and 30 days, wherein the preservation number is as follows: CGMCC No. 17863.
An Alcaligenes faecalis, the 16S rDNA of which is shown in SEQ ID No.1.
Correspondingly, the alcaligenes faecalis is applied to flocculation of chicken manure biogas slurry.
Preferably, iron sulfate is used as a coagulant aid in the application.
Preferably, the amount of the ferric sulfate is
The mass g of the coagulant aid is per the total volume mL of the flocculation system.
Preferably, the application is: adding the alcaligenes faecalis fermentation product into the chicken manure biogas slurry, adding the coagulant aid, uniformly mixing, and standing.
Preferably, the fermentation product of the alcaligenes faecalis is a supernatant of an alcaligenes faecalis fermentation broth.
Preferably, the fermentation product of the alcaligenes faecalis is taken from the fermentation broth of day 3 of fermentation.
Preferably, the alcaligenes faecalis uses glucose or sodium acetate as a carbon source during fermentation.
Preferably, the alcaligenes faecalis uses peptone as a nitrogen source during fermentation.
The invention has the following beneficial effects: the invention provides a novel alcaligenes faecalis, and a flocculating agent capable of performing targeted flocculation treatment on chicken manure biogas slurry is prepared by using supernatant obtained by fermenting the alcaligenes faecalis.
Drawings
FIG. 1 is a graph showing the effect of flocculating a kaolin solution using fermentation broths of different fermentation times;
FIG. 2 is a graph showing the effect of flocculating a kaolin solution using different coagulant aids;
FIG. 3 is a graph showing the effect of flocculating a kaolin solution using different types of flocculants;
FIG. 4 is a graph showing the effect of using different external carbon sources on a flocculated kaolin solution;
FIG. 5 is a graph showing the effect of using different added nitrogen sources on a flocculated kaolin solution;
FIG. 6 is a diagram showing the effect of coagulant aid dosage on flocculation of chicken manure biogas slurry;
FIG. 7 is a diagram showing the effect of the usage amount of the fermentation liquid on the flocculation of the chicken manure biogas slurry;
FIG. 8 is a diagram showing the effect of the fermentation liquid in flocculating different batches of chicken manure biogas slurry;
FIG. 9 is a photograph of chicken manure biogas slurry flocculated by fermentation broth in different batches;
FIG. 10 is a photograph of cow dung and biogas slurry flocculated by fermentation broth;
FIG. 11 is a photograph of a fermentation broth flocculated pig manure biogas slurry;
FIG. 12 is a photograph of a fermentation broth flocculated whole stillage biogas slurry;
FIG. 13 is a photograph comparing flocculation of chicken manure and biogas slurry with different flocculants;
FIG. 14 is a photograph of chicken manure biogas slurry flocculated by other Alcaligenes faecalis.
Detailed Description
The formula of the culture medium is as follows: 10g/L of glucose; peptone 0.5g/L, yeast extract 0.5g/L, urea 0.5g/L, (NH)4)2SO40.5g/L，KH2PO45g/L，MgSO4·7H20.2g/L of O, 0.1g/L of NaCl, 7-9 of pH and 20min of sterilization at 115 ℃. If a solid medium is required, 20g/L agar is added.
The experimental method for flocculating the kaolin solution comprises the following steps: taking 40mL of 4g/L kaolin solution, adding a flocculating agent and a coagulant aid, stirring uniformly, standing for 10min, and observing the flocculation effect. The calculation method of the flocculation rate comprises the following steps: and respectively measuring the change of the absorbance values before and after the treatment of the kaolin by adopting a spectrophotometer method so as to calculate the flocculation rate. The method specifically comprises the following steps: and (3) taking liquid 2cm below the liquid surface, measuring the absorbance value of the liquid at 550nm, and obtaining the flocculation rate according to the change of the absorbance value of the liquid after the flocculant is added and the blank. The calculation formula is as follows: the flocculation rate of kaolin is (A-B)/A multiplied by 100%. Wherein A is the absorbance value of blank group at 550nm, and B is the absorbance value at 550nm after flocculant is added for treatment.
The experimental method for flocculating the biogas slurry comprises the following steps: and (3) adding a flocculating agent and a coagulant aid into 20mL of biogas slurry, uniformly stirring, standing for 10min, and observing the flocculation effect. The calculation method of the flocculation rate comprises the following steps: and measuring the change of turbidity before and after biogas slurry treatment by using a turbidity meter to calculate. The method specifically comprises the following steps: the liquid 2cm below the liquid surface was taken, and the change in turbidity before and after the treatment was measured, whereby the flocculation rate was calculated. The calculation formula is as follows: the flocculation rate of the biogas slurry is (M-N)/M multiplied by 100 percent. Wherein M is the turbidity of the blank group, and N is the turbidity after the flocculant is added.
The present invention will be further explained with reference to specific examples.
The first embodiment is as follows: screening and identification of flocculant-producing bacteria
Taking 10mL of activated sludge stored in a fermentation tank of a laboratory of Chengdu biological research institute of Chinese academy of sciences to a 150mL triangular flask filled with 50mL of sterile water, shaking uniformly, standing for 1min, taking 5mL of activated sludge to a 150mL triangular flask filled with 100mL of the culture medium, and carrying out shake culture at the temperature of 160r/min and the pH of 7 for 24 h. Followed by dilution coating to give a coating composition 10-1、10-2、10-3、10-4、10-5、10-6And coating the six gradient diluted fermentation liquors on the solid culture medium, selecting different single colonies, and separating the single colonies for more than three times by using a plate marking method until the colony forms are consistent to obtain the purified strain. Inoculating 5mL of the purified strain into a 150mL triangular flask filled with 100mL of the culture medium, performing shake culture at the temperature of 160r/min and the pH value of 7 for 72h at 30 ℃, sampling every 24h, taking a sample by taking a common coagulant aid calcium chloride as the coagulant aid, measuring the flocculation rate of a treated object which is the chicken manure biogas slurry, and selecting the strain with good flocculation effect.
Sequencing the obtained strains: a bacterial whole genome rapid extraction kit is adopted to extract the whole genome of a pure strain, PCR amplification is carried out by selecting a bacterial 16S rRNA universal primer, and then sequencing analysis is carried out, wherein the 16S rRNA gene sequence is shown as SEQ ID No.1. The sequencing result shows that the homology of the strain AS28 and Alcaligenes faecalis is 100% by BLAST comparison in NCBI database, so that the strain is identified AS Alcaligenes faecalis.
The alcaligenes faecalis named as: alcaligenes faecalis (Alcaligenes faecalis) deposited in the general microbiological culture Collection center of the China Committee for culture Collection of microorganisms at 30/5 in 2019, address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101, accession number: CGMCC No. 17863.
Example two: condition optimization of alcaligenes faecalis flocculating kaolin solution
The same culture medium is prepared and is respectively filled in a 150mL triangular flask, the Alcaligenes faecalis is inoculated for culture, kaolin is taken as a processing object, fermentation liquor is taken as a flocculating agent, and the influence of different flocculation aids on the growth and flocculation effect of thalli at different time is investigated by adopting a single-factor test in a kaolin suspension system of 40mL and 4 g/L. Apart from the factors considered, the fermentation conditions were: shaking-culturing at pH 7, 30 deg.C and 160r/min for 3 days. The dosage of the flocculating agent is 2 percent (% refers to the volume ratio between the flocculating agent and the biogas slurry to be flocculated, and the same is true when no special description is given later, and 2 percent is the general dosage). The method comprises the following specific steps:
1. and (4) optimizing the fermentation time (bacterial concentration) of the flocculant. One ten-thousandth of the dosage of ferric sulfate is used as coagulant aid, shaking culture is carried out for 7d at 160r/min, the fermentation liquor is sampled once every 24h and used as flocculant, and the flocculation rate is measured. The results are shown in FIG. 1. In the case of using the coagulant aid, the culture was continued for 5 days (viable bacteria concentration in the fermentation broth was 1.8X 10)8CFU/mL or so; OD of fermentation broth6002) best flocculation effect, day 3 (viable bacteria concentration in fermentation broth is 2X 10)8CFU/mL or so; OD of fermentation broth6001.7) is equivalent to the effect on day 5. In practical application, the fermentation is carried out until the concentration of viable bacteria in the fermentation liquid is 1 × 106CFU/mL～5×108CFU/mL, and OD of fermentation broth 6001 to 2.5. The concentration of viable bacteria is 1.8 multiplied by 108CFU/mL, OD of fermentation broth600When the amount is 2, the flocculation effect is the best. Considering that the cost is too high when the culture time is too long in practical use, the fermentation liquid of the third day is selected as the flocculating agent in the subsequent experiments.
2. And (4) optimizing the coagulant aid. And (3) taking the fermentation liquor fermented to the 3 rd day in the step (1), respectively selecting aluminum sulfate, aluminum chloride, ferric sulfate, calcium sulfate and calcium chloride as coagulant aids, wherein the addition amount of each coagulant aid is one ten thousandth (mass g of the coagulant aid/total volume mL of a flocculation system). The flocculation rate was measured. The results are shown in FIG. 2, and the flocculation effect is best when ferric sulfate is selected as the coagulant aid.
3. And (4) selecting the type of the flocculating agent. And (3) fermenting for 3 days in the step (1) to obtain a fermentation liquid, taking the centrifugal supernatant of the fermentation liquid, the centrifugal precipitate of the fermentation liquid, the centrifugal supernatant after the centrifugal precipitate of the fermentation liquid is crushed and the centrifugal precipitate after the centrifugal precipitate of the fermentation liquid is crushed, respectively using the centrifugal supernatant and the centrifugal precipitate as flocculating agents, and respectively measuring the flocculation rate by using one ten thousandth of ferric sulfate as a coagulant aid. The results are shown in FIG. 3. Subsequent experiments all used the centrifuged supernatant of the fermentation broth as flocculant.
4. And (4) optimizing an external carbon source. 10g/L glucose, 10g/L xylose, 9.5g/L sucrose, 9.5g/L soluble starch, 14.3g/L sodium citrate, 7.7g/L ethanol, 13.7 g/L sodium acetate and 10.7g/L sodium propionate are used as external carbon sources to respectively replace 10g/L glucose in the culture medium. And respectively culturing the alcaligenes faecalis by using the adjusted new culture medium. The flocculation rate of each group was measured separately. The results are shown in FIG. 4.
5. Optimizing the additional nitrogen source. The nitrogen sources (peptone 0.5g/L, yeast extract 0.5g/L, urea 0.5g/L and (NH) in the medium were replaced with 3.2g/L peptone, 1.8g/L ammonium chloride, 3.3g/L potassium nitrate and 1.0g/L urea, respectively, as additional nitrogen sources4)2SO40.5 g/L). The total amount of the additional nitrogen source is as follows: 0.5g/L of N. The flocculation rate of each group was measured separately and the results are shown in fig. 5.
According to the above test, it was finally determined that, in the subsequent test, the fermentation broth of the Alcaligenes faecalis on the third day was taken AS a flocculant (hereinafter, this flocculant will be referred to simply AS AS 28); ferric sulfate is used as coagulant aid; the growth is good when sucrose is used as an external carbon source and peptone is used as an external nitrogen source; the flocculation rate is highest when glucose or sodium acetate is used as an external carbon source and peptone is used as an external nitrogen source, and reaches 84.76%.
Example three: condition optimization of chicken manure biogas slurry flocculated by alcaligenes faecalis
Selecting chicken manure biogas slurry of chicken manure biogas engineering in Shandong for flocculation test. The initial ammonia nitrogen content in the chicken manure biogas slurry is about 5140mg/L, and the COD is 15000-17000 mg/L. And preparing the same culture medium, subpackaging in a triangular flask with 150mL, and inoculating alcaligenes faecalis for culture. The method is characterized in that the chicken manure biogas slurry is taken as a treatment object, the centrifugal supernatant of fermentation liquor is taken as a flocculating agent, and the influence of different coagulant aid dosage and different flocculating agent dosage on the growth and flocculation effect of thalli is examined by adopting a single-factor test in a 40mL chicken manure biogas slurry system. The fermentation conditions for obtaining the fermentation liquor are as follows: shaking-culturing at pH 7, 30 deg.C and 160r/min for 3 days. The method comprises the following specific steps:
1. and optimizing the dosage of the coagulant aid. The dosage of the flocculating agent is 3 percent (the volume of the flocculating agent is the volume of the biogas slurry to be flocculated). Ferric sulfate as coagulant aid is added separately
The coagulant aid of (1). Wherein, the ratio specifically refers to the mass of the coagulant aid: total system volume (g/mL). For convenient handling, 400. mu.L of 1.000% coagulant aid solution was prepared. The flocculation rate of each group is measured. The results are shown in FIG. 6: adding
The flocculation effect is best when the coagulant aid is used.
2. And optimizing the dosage of the flocculating agent. Use of
1%, 2%, 3%, 4% and 5% of flocculating agent is added into the ferric sulfate solution. The flocculant refers to supernatant of fermentation liquor obtained in the step 1 and fermented for 3 days. Wherein, the ratio refers to the volume of the flocculating agent: and (5) the volume of the biogas slurry to be flocculated. The flocculation rate was measured separately. As shown in FIG. 7, 0.4, 0.8, 1.2, 1.6, and 2.0mL of the supernatant of the fermentation broth was used as a flocculant in FIG. 7. FIG. 7 shows that the flocculation effect is best when the dosage of the flocculating agent is 3%.
Example four: effect display of chicken manure biogas slurry from different sources by flocculating alkali producing bacteria
Selecting chicken manure biogas slurry of different batches in chicken manure biogas engineering of Shandong,a flocculation test was performed. The test method is the same as the third embodiment, wherein the dosage of ferric sulfate is
The dosage of the flocculant is 3 percent. The results are shown in FIG. 8, where the flocculation rates were 85.79%, 83.87%, 79.97%, 87.68%, 90.32%, and 71.46%, respectively. And the effect of 2 bottles of flocculated chicken manure biogas slurry was arbitrarily selected for display, as shown in fig. 9. The method proves that the alcaligenes faecalis has a targeted flocculation effect on the chicken manure biogas slurry and is independent of the batch of the chicken manure biogas slurry.
Example five: demonstration of effect of flocculation of other biogas slurry by alcaligenes faecalis
And flocculating the cow dung biogas slurry, the pig dung biogas slurry and the vinasse biogas slurry respectively by using supernatant of the alcaligenes faecalis fermentation liquid fermented for 3 days as a flocculating agent and using ferric sulfate as a coagulant aid under the same conditions as in the fourth embodiment. The cow dung biogas slurry is obtained from biogas engineering of a certain dairy farm of Shijiazhuang; the pig manure biogas slurry is taken from biogas engineering of a certain pig farm in a Chengdu dual-flow area; the vinasse biogas slurry is obtained from a vinasse biogas project in a Zunyi sowing area.
The flocculation effects of the cow dung biogas slurry, the pig dung biogas slurry and the vinasse biogas slurry are respectively shown in figures 10, 11 and 12, and the flocculation rates are respectively 3.27%, 5.96% and 2.17%. The results prove that the flocculation effect of the Alcaligenes faecalis on the biogas slurry is mainly reflected on chicken manure biogas slurry, and if the type of the biogas slurry is changed, a good flocculation effect cannot be achieved. The possible reasons are: specific reaction is generated between certain or certain specific substances contained in the chicken manure biogas slurry and the alcaligenes faecalis or metabolites thereof provided by the invention, so that an excellent targeted flocculation effect is achieved.
Example six: effect display of other flocculating agent flocculating chicken manure biogas slurry
1. Under the same conditions AS the fourth example, the flocculant of alcaligenes faecalis (AS28) + ferric sulfate coagulant aid, flocculant AS30 (produced from Brevundimonas diminuta, CGMCC No.17864) + ferric sulfate coagulant aid, flocculant AS33 (produced from Bacillus cereus, CGMCC No.17865) + ferric sulfate coagulant aid, ferric sulfate, polyaluminium chloride and polyacrylamide of the invention were used AS flocculants respectively to flocculate the chicken manure biogas slurry. As shown in FIG. 13, the flocculation rates were 83.87%, 3.27%, 4.51%, 6.44%, 24.31%, and 19.75%, respectively.
2. CGMCC1 No.16044 Alcaligenes faecalis (Alcaligenes faecalis) and CGMCC No.1.924 Alcaligenes faecalis (Alcaligenes faecalis) are purchased from the China center for culture collection and management of microorganisms, and ferric sulfate coagulant aids are added to flocculate the chicken manure and biogas slurry under the same conditions as in the fourth embodiment. The results are shown in FIG. 14, where the flocculation rates were 6.37% and 24.15%, respectively. The effect is not ideal.
The tests prove that the AS28 provided by the invention has a targeted flocculation effect on the chicken manure biogas slurry, and the flocculant prepared by other microorganisms or a common chemical flocculant is difficult to achieve a comparable effect.
Sequence listing
<110> institute of biological research of Chengdu of Chinese academy of sciences
<120> Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1470
<212>DNA
<213> Alcaligenes faecalis (Alcaligenes faecalis)
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ttgctctctt ggtggcgagt ggcggacggg tgagtaatat atcggaacgt gcccagtagc 120
gggggataac tactcgaaag agtggctaat accgcatacg ccctacgggg gaaagggggg 180
gatcgcaaga cctctcacta ttggagcggc cgatatcgga ttagctagtt ggtggggtaa 240
aggctcacca aggcaacgat ccgtagctgg tttgagagga cgaccagcca cactgggact 300
gagacacggc ccagactcct acgggaggca gcagtgggga attttggaca atgggggaaa 360
ccctgatcca gccatcccgc gtgtatgatg aaggccttcg ggttgtaaag tacttttggc 420
agagaagaaa aggcatcccc taatacggga tgctgctgac ggtatctgca gaataagcac 480
cggctaacta cgtgccagca gccgcggtaa tacgtagggt gcaagcgtta atcggaatta 540
ctgggcgtaa agcgtgtgta ggcggttcgg aaagaaagat gtgaaatccc agggctcaac 600
cttggaactg catttttaac tgccgagcta gagtatgtca gaggggggta gaattccacg 660
tgtagcagtg aaatgcgtag atatgtggag gaataccgat ggcgaaggca gccccctggg 720
ataatactga cgctcagaca cgaaagcgtg gggagcaaac aggattagat accctggtag 780
tccacgccct aaacgatgtc aactagctgt tggggccgtt aggccttagt agcgcagcta 840
acgcgtgaag ttgaccgcct ggggagtacg gtcgcaagat taaaactcaa aggaattgac 900
ggggacccgc acaagcggtg gatgatgtgg attaattcga tgcaacgcga aaaaccttac 960
ctacccttga catgtctgga atgccgaaga gatttggcag tgctcgcaag agaaccggaa 1020
cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1080
cgagcgcaac ccttgtcatt agttgctacg caagagcact ctaatgagac tgccggtgac 1140
aaaccggagg aaggtgggga tgacgtcaag tcctcatggc ccttatgggt agggcttcac 1200
acgtcataca atggtcggga cagagggtcg ccaacccgcg agggggagcc aatctcagaa 1260
acccgatcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg aatcgctagt 1320
aatcgcggat cagaatgtcg cggtgaatac gttcccgggt cttgtacaca ccgcccgtca 1380
caccatggga gtgggtttca ccagaagtag gtagcctaac cgtaaggagg gcgcttacca 1440
cggtgggatt catgactggg gtgaagtcgt 1470
Claims (10)
1. An alcaligenes faecalis strain, which is characterized in that: and in 2019, 5, month and 30, the strain is preserved in the China general microbiological culture Collection center (CGMCC), and the preservation numbers are as follows: CGMCC No. 17863.
2. An alcaligenes faecalis strain, which is characterized in that: the 16S rDNA is shown in SEQ ID No.1.
3. Use of the alcaligenes faecalis according to claim 1 or 2 for flocculating chicken manure biogas slurry.
4. The use of the alcaligenes faecalis according to claim 3 in flocculating chicken manure biogas slurry, characterized in that: ferric sulfate is used as a coagulant aid in the application.
5. The use of the alcaligenes faecalis according to claim 4 in flocculating chicken manure biogas slurry, characterized in that: the dosage of the ferric sulfate is
The mass g of the coagulant aid is per the total volume mL of the flocculation system.
6. The use of the alcaligenes faecalis according to claim 4 in flocculating chicken manure biogas slurry, characterized in that: the application is as follows: adding the alcaligenes faecalis fermentation product into the chicken manure biogas slurry, adding the coagulant aid, uniformly mixing, and standing.
7. The use of the alcaligenes faecalis of claim 6 in flocculating chicken manure biogas slurry, characterized in that: the fermentation product of the alcaligenes faecalis is supernatant of fermentation liquor of the alcaligenes faecalis.
8. The use of the alcaligenes faecalis of claim 7 in flocculating chicken manure biogas slurry, characterized in that: the fermentation product of the alcaligenes faecalis is taken from fermentation liquor fermented on the 3 rd day.
9. The use of the alcaligenes faecalis of claim 6 in flocculating chicken manure biogas slurry, characterized in that: in the fermentation process of the Alcaligenes faecalis, glucose or sodium acetate is used as a carbon source.
10. The use of the alcaligenes faecalis of claim 6 in flocculating chicken manure biogas slurry, characterized in that: during the fermentation process of the Alcaligenes faecalis, peptone is used as a nitrogen source.
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