CN111233167B - Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation - Google Patents
Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation Download PDFInfo
- Publication number
- CN111233167B CN111233167B CN202010069156.0A CN202010069156A CN111233167B CN 111233167 B CN111233167 B CN 111233167B CN 202010069156 A CN202010069156 A CN 202010069156A CN 111233167 B CN111233167 B CN 111233167B
- Authority
- CN
- China
- Prior art keywords
- biogas slurry
- alcaligenes faecalis
- chicken manure
- flocculation
- manure biogas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002002 slurry Substances 0.000 title claims abstract description 80
- 241000588813 Alcaligenes faecalis Species 0.000 title claims abstract description 60
- 229940005347 alcaligenes faecalis Drugs 0.000 title claims abstract description 60
- 238000005189 flocculation Methods 0.000 title claims abstract description 60
- 230000016615 flocculation Effects 0.000 title claims abstract description 60
- 210000003608 fece Anatomy 0.000 title claims abstract description 52
- 239000010871 livestock manure Substances 0.000 title claims abstract description 46
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 44
- 238000000855 fermentation Methods 0.000 claims abstract description 53
- 230000004151 fermentation Effects 0.000 claims abstract description 53
- 239000000701 coagulant Substances 0.000 claims abstract description 34
- 238000002156 mixing Methods 0.000 claims abstract description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 230000003311 flocculating effect Effects 0.000 claims description 17
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 17
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 17
- 239000006228 supernatant Substances 0.000 claims description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 239000001888 Peptone Substances 0.000 claims description 7
- 108010080698 Peptones Proteins 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 235000019319 peptone Nutrition 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000000047 product Substances 0.000 claims description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 36
- 238000000034 method Methods 0.000 abstract description 12
- 244000005700 microbiome Species 0.000 abstract description 4
- 239000008394 flocculating agent Substances 0.000 description 28
- 239000007788 liquid Substances 0.000 description 16
- 239000005995 Aluminium silicate Substances 0.000 description 12
- 235000012211 aluminium silicate Nutrition 0.000 description 12
- 235000010633 broth Nutrition 0.000 description 12
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 12
- 239000000243 solution Substances 0.000 description 10
- 239000001963 growth medium Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229920000620 organic polymer Polymers 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 229920000592 inorganic polymer Polymers 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000589539 Brevundimonas diminuta Species 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 206010043275 Teratogenicity Diseases 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
- -1 aluminum ions Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000008199 coating composition Substances 0.000 description 1
- 230000001609 comparable effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 231100000568 intoxicate Toxicity 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 1
- 239000004324 sodium propionate Substances 0.000 description 1
- 235000010334 sodium propionate Nutrition 0.000 description 1
- 229960003212 sodium propionate Drugs 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920005613 synthetic organic polymer Polymers 0.000 description 1
- 231100000211 teratogenicity Toxicity 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Treatment Of Sludge (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention belongs to the field of microorganisms, and particularly relates to an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation. The concrete application is as follows: adding the alcaligenes faecalis fermentation product into the chicken manure biogas slurry, adding the coagulant aid, uniformly mixing, and standing. By using the method, the chicken manure biogas slurry can be specifically flocculated, and the flocculation effect is up to more than 90%.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation.
Background
In recent years, with the continuous expansion of livestock and poultry breeding scale, the breeding sewage discharge amount is increased day by day. If the culture wastewater is directly discharged into the environment, serious environmental problems are caused. Anaerobic digestion is a common method for treating breeding sewage, can realize harmless treatment of livestock and poultry breeding wastes, and can also generate biogas and provide clean energy. Meanwhile, along with the rapid development of biogas engineering, a large amount of biogas slurry is generated. But the biogas slurry has the defects of high water content, difficult storage and transportation, relatively low economic value and the like. When the engineering scale is too large and the produced biogas slurry amount far exceeds the maximum consumption of the local farmland, the biogas slurry becomes wastewater which is difficult to treat. If the part of biogas slurry is discharged randomly, the natural environment, especially the water body environment, can be seriously damaged. Therefore, it is important to perform harmless treatment on the biogas slurry to make the biogas slurry reach relevant standards. However, the existing treatment methods for biogas slurry all require flocculation and precipitation to remove particle suspended matters (SS) in the biogas slurry.
The flocculating agents widely used at present mainly comprise: inorganic polymeric flocculants typified by aluminum, and organic polymeric flocculants typified by polyacrylamide. The inorganic flocculant has the advantages of economy and simple use method; but also has the defects of large dosage, low flocculation effect, high cost, strong corrosivity and the like. The organic polymer flocculant is a novel wastewater treatment agent developed in the later stage of the 60 th century, and compared with the traditional flocculant, the organic polymer flocculant can improve the efficiency by times, has small using amount, low scum yield, strong flocculation capacity, easy separation of flocs and good oil removal and suspended matter removal effects, and is suitable for the treatment of wastewater in the later stage of the 20 th century. However, most organic polymeric flocculants are toxic or hydrolysis and degradation products thereof, and the acrylamide monomers used for synthesis are toxic, so that the organic polymeric flocculants can intoxicate the central nerve of people, and residual monomers have 'triple-effect' (teratogenicity, carcinogenicity and mutagenicity), thereby being not in line with the current trend of environment-friendly development. In addition, when inorganic polymers or organic polymers are used as the flocculant, the presence of a large amount of aluminum ions or polyacrylamide in the precipitate may also affect the use of the biogas residues or biogas slurry as a high-quality fertilizer.
Because the microbial flocculant can overcome the inherent defects of inorganic polymer and synthetic organic polymer flocculants, has no secondary pollution and safe use, the research on the microbial flocculant is increasingly emphasized in recent years. However, the biogas slurry has a certain sterilization effect, and microbial strains which can grow well in the biogas slurry are deficient in resources, so that the flocculation effect is not ideal. In addition, due to different components of different biogas slurries, a strain which can grow well in one biogas slurry does not necessarily adapt to the environment of another biogas slurry. Therefore, microbial flocculants applicable to biogas slurry have been limited in development. If the microbial flocculant with a specific flocculation effect can be provided for specific biogas slurry, the method has important practical significance.
Disclosure of Invention
The invention aims to provide an alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows: an alcaligenes faecalis preserved in the China general microbiological culture Collection center in 2019, 5 months and 30 days, wherein the preservation number is as follows: CGMCC No. 17863.
An Alcaligenes faecalis, the 16S rDNA of which is shown in SEQ ID No.1.
Correspondingly, the alcaligenes faecalis is applied to flocculation of chicken manure biogas slurry.
Preferably, iron sulfate is used as a coagulant aid in the application.
Preferably, the amount of the ferric sulfate isThe mass g of the coagulant aid is per the total volume mL of the flocculation system.
Preferably, the application is: adding the alcaligenes faecalis fermentation product into the chicken manure biogas slurry, adding the coagulant aid, uniformly mixing, and standing.
Preferably, the fermentation product of the alcaligenes faecalis is a supernatant of an alcaligenes faecalis fermentation broth.
Preferably, the fermentation product of the alcaligenes faecalis is taken from the fermentation broth of day 3 of fermentation.
Preferably, the alcaligenes faecalis uses glucose or sodium acetate as a carbon source during fermentation.
Preferably, the alcaligenes faecalis uses peptone as a nitrogen source during fermentation.
The invention has the following beneficial effects: the invention provides a novel alcaligenes faecalis, and a flocculating agent capable of performing targeted flocculation treatment on chicken manure biogas slurry is prepared by using supernatant obtained by fermenting the alcaligenes faecalis.
Drawings
FIG. 1 is a graph showing the effect of flocculating a kaolin solution using fermentation broths of different fermentation times;
FIG. 2 is a graph showing the effect of flocculating a kaolin solution using different coagulant aids;
FIG. 3 is a graph showing the effect of flocculating a kaolin solution using different types of flocculants;
FIG. 4 is a graph showing the effect of using different external carbon sources on a flocculated kaolin solution;
FIG. 5 is a graph showing the effect of using different added nitrogen sources on a flocculated kaolin solution;
FIG. 6 is a diagram showing the effect of coagulant aid dosage on flocculation of chicken manure biogas slurry;
FIG. 7 is a diagram showing the effect of the usage amount of the fermentation liquid on the flocculation of the chicken manure biogas slurry;
FIG. 8 is a diagram showing the effect of the fermentation liquid in flocculating different batches of chicken manure biogas slurry;
FIG. 9 is a photograph of chicken manure biogas slurry flocculated by fermentation broth in different batches;
FIG. 10 is a photograph of cow dung and biogas slurry flocculated by fermentation broth;
FIG. 11 is a photograph of a fermentation broth flocculated pig manure biogas slurry;
FIG. 12 is a photograph of a fermentation broth flocculated whole stillage biogas slurry;
FIG. 13 is a photograph comparing flocculation of chicken manure and biogas slurry with different flocculants;
FIG. 14 is a photograph of chicken manure biogas slurry flocculated by other Alcaligenes faecalis.
Detailed Description
The formula of the culture medium is as follows: 10g/L of glucose; peptone 0.5g/L, yeast extract 0.5g/L, urea 0.5g/L, (NH)4)2SO4 0.5g/L,KH2PO4 5g/L,MgSO4·7H20.2g/L of O, 0.1g/L of NaCl, 7-9 of pH and 20min of sterilization at 115 ℃. If a solid medium is required, 20g/L agar is added.
The experimental method for flocculating the kaolin solution comprises the following steps: taking 40mL of 4g/L kaolin solution, adding a flocculating agent and a coagulant aid, stirring uniformly, standing for 10min, and observing the flocculation effect. The calculation method of the flocculation rate comprises the following steps: and respectively measuring the change of the absorbance values before and after the treatment of the kaolin by adopting a spectrophotometer method so as to calculate the flocculation rate. The method specifically comprises the following steps: and (3) taking liquid 2cm below the liquid surface, measuring the absorbance value of the liquid at 550nm, and obtaining the flocculation rate according to the change of the absorbance value of the liquid after the flocculant is added and the blank. The calculation formula is as follows: the flocculation rate of kaolin is (A-B)/A multiplied by 100%. Wherein A is the absorbance value of blank group at 550nm, and B is the absorbance value at 550nm after flocculant is added for treatment.
The experimental method for flocculating the biogas slurry comprises the following steps: and (3) adding a flocculating agent and a coagulant aid into 20mL of biogas slurry, uniformly stirring, standing for 10min, and observing the flocculation effect. The calculation method of the flocculation rate comprises the following steps: and measuring the change of turbidity before and after biogas slurry treatment by using a turbidity meter to calculate. The method specifically comprises the following steps: the liquid 2cm below the liquid surface was taken, and the change in turbidity before and after the treatment was measured, whereby the flocculation rate was calculated. The calculation formula is as follows: the flocculation rate of the biogas slurry is (M-N)/M multiplied by 100 percent. Wherein M is the turbidity of the blank group, and N is the turbidity after the flocculant is added.
The present invention will be further explained with reference to specific examples.
The first embodiment is as follows: screening and identification of flocculant-producing bacteria
Taking 10mL of activated sludge stored in a fermentation tank of a laboratory of Chengdu biological research institute of Chinese academy of sciences to a 150mL triangular flask filled with 50mL of sterile water, shaking uniformly, standing for 1min, taking 5mL of activated sludge to a 150mL triangular flask filled with 100mL of the culture medium, and carrying out shake culture at the temperature of 160r/min and the pH of 7 for 24 h. Followed by dilution coating to give a coating composition 10-1、10-2、10-3、10-4、10-5、10-6And coating the six gradient diluted fermentation liquors on the solid culture medium, selecting different single colonies, and separating the single colonies for more than three times by using a plate marking method until the colony forms are consistent to obtain the purified strain. Inoculating 5mL of the purified strain into a 150mL triangular flask filled with 100mL of the culture medium, performing shake culture at the temperature of 160r/min and the pH value of 7 for 72h at 30 ℃, sampling every 24h, taking a sample by taking a common coagulant aid calcium chloride as the coagulant aid, measuring the flocculation rate of a treated object which is the chicken manure biogas slurry, and selecting the strain with good flocculation effect.
Sequencing the obtained strains: a bacterial whole genome rapid extraction kit is adopted to extract the whole genome of a pure strain, PCR amplification is carried out by selecting a bacterial 16S rRNA universal primer, and then sequencing analysis is carried out, wherein the 16S rRNA gene sequence is shown as SEQ ID No.1. The sequencing result shows that the homology of the strain AS28 and Alcaligenes faecalis is 100% by BLAST comparison in NCBI database, so that the strain is identified AS Alcaligenes faecalis.
The alcaligenes faecalis named as: alcaligenes faecalis (Alcaligenes faecalis) deposited in the general microbiological culture Collection center of the China Committee for culture Collection of microorganisms at 30/5 in 2019, address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101, accession number: CGMCC No. 17863.
Example two: condition optimization of alcaligenes faecalis flocculating kaolin solution
The same culture medium is prepared and is respectively filled in a 150mL triangular flask, the Alcaligenes faecalis is inoculated for culture, kaolin is taken as a processing object, fermentation liquor is taken as a flocculating agent, and the influence of different flocculation aids on the growth and flocculation effect of thalli at different time is investigated by adopting a single-factor test in a kaolin suspension system of 40mL and 4 g/L. Apart from the factors considered, the fermentation conditions were: shaking-culturing at pH 7, 30 deg.C and 160r/min for 3 days. The dosage of the flocculating agent is 2 percent (% refers to the volume ratio between the flocculating agent and the biogas slurry to be flocculated, and the same is true when no special description is given later, and 2 percent is the general dosage). The method comprises the following specific steps:
1. and (4) optimizing the fermentation time (bacterial concentration) of the flocculant. One ten-thousandth of the dosage of ferric sulfate is used as coagulant aid, shaking culture is carried out for 7d at 160r/min, the fermentation liquor is sampled once every 24h and used as flocculant, and the flocculation rate is measured. The results are shown in FIG. 1. In the case of using the coagulant aid, the culture was continued for 5 days (viable bacteria concentration in the fermentation broth was 1.8X 10)8CFU/mL or so; OD of fermentation broth6002) best flocculation effect, day 3 (viable bacteria concentration in fermentation broth is 2X 10)8CFU/mL or so; OD of fermentation broth6001.7) is equivalent to the effect on day 5. In practical application, the fermentation is carried out until the concentration of viable bacteria in the fermentation liquid is 1 × 106CFU/mL~5×108CFU/mL, and OD of fermentation broth 6001 to 2.5. The concentration of viable bacteria is 1.8 multiplied by 108CFU/mL, OD of fermentation broth600When the amount is 2, the flocculation effect is the best. Considering that the cost is too high when the culture time is too long in practical use, the fermentation liquid of the third day is selected as the flocculating agent in the subsequent experiments.
2. And (4) optimizing the coagulant aid. And (3) taking the fermentation liquor fermented to the 3 rd day in the step (1), respectively selecting aluminum sulfate, aluminum chloride, ferric sulfate, calcium sulfate and calcium chloride as coagulant aids, wherein the addition amount of each coagulant aid is one ten thousandth (mass g of the coagulant aid/total volume mL of a flocculation system). The flocculation rate was measured. The results are shown in FIG. 2, and the flocculation effect is best when ferric sulfate is selected as the coagulant aid.
3. And (4) selecting the type of the flocculating agent. And (3) fermenting for 3 days in the step (1) to obtain a fermentation liquid, taking the centrifugal supernatant of the fermentation liquid, the centrifugal precipitate of the fermentation liquid, the centrifugal supernatant after the centrifugal precipitate of the fermentation liquid is crushed and the centrifugal precipitate after the centrifugal precipitate of the fermentation liquid is crushed, respectively using the centrifugal supernatant and the centrifugal precipitate as flocculating agents, and respectively measuring the flocculation rate by using one ten thousandth of ferric sulfate as a coagulant aid. The results are shown in FIG. 3. Subsequent experiments all used the centrifuged supernatant of the fermentation broth as flocculant.
4. And (4) optimizing an external carbon source. 10g/L glucose, 10g/L xylose, 9.5g/L sucrose, 9.5g/L soluble starch, 14.3g/L sodium citrate, 7.7g/L ethanol, 13.7 g/L sodium acetate and 10.7g/L sodium propionate are used as external carbon sources to respectively replace 10g/L glucose in the culture medium. And respectively culturing the alcaligenes faecalis by using the adjusted new culture medium. The flocculation rate of each group was measured separately. The results are shown in FIG. 4.
5. Optimizing the additional nitrogen source. The nitrogen sources (peptone 0.5g/L, yeast extract 0.5g/L, urea 0.5g/L and (NH) in the medium were replaced with 3.2g/L peptone, 1.8g/L ammonium chloride, 3.3g/L potassium nitrate and 1.0g/L urea, respectively, as additional nitrogen sources4)2SO40.5 g/L). The total amount of the additional nitrogen source is as follows: 0.5g/L of N. The flocculation rate of each group was measured separately and the results are shown in fig. 5.
According to the above test, it was finally determined that, in the subsequent test, the fermentation broth of the Alcaligenes faecalis on the third day was taken AS a flocculant (hereinafter, this flocculant will be referred to simply AS AS 28); ferric sulfate is used as coagulant aid; the growth is good when sucrose is used as an external carbon source and peptone is used as an external nitrogen source; the flocculation rate is highest when glucose or sodium acetate is used as an external carbon source and peptone is used as an external nitrogen source, and reaches 84.76%.
Example three: condition optimization of chicken manure biogas slurry flocculated by alcaligenes faecalis
Selecting chicken manure biogas slurry of chicken manure biogas engineering in Shandong for flocculation test. The initial ammonia nitrogen content in the chicken manure biogas slurry is about 5140mg/L, and the COD is 15000-17000 mg/L. And preparing the same culture medium, subpackaging in a triangular flask with 150mL, and inoculating alcaligenes faecalis for culture. The method is characterized in that the chicken manure biogas slurry is taken as a treatment object, the centrifugal supernatant of fermentation liquor is taken as a flocculating agent, and the influence of different coagulant aid dosage and different flocculating agent dosage on the growth and flocculation effect of thalli is examined by adopting a single-factor test in a 40mL chicken manure biogas slurry system. The fermentation conditions for obtaining the fermentation liquor are as follows: shaking-culturing at pH 7, 30 deg.C and 160r/min for 3 days. The method comprises the following specific steps:
1. and optimizing the dosage of the coagulant aid. Amount of flocculantIs 3 percent (the volume of the flocculating agent is the volume of the biogas slurry to be flocculated). Ferric sulfate as coagulant aid is added separately The coagulant aid of (1). Wherein, the ratio specifically refers to the mass of the coagulant aid: total system volume (g/mL). For convenient handling, 400. mu.L of 1.000% coagulant aid solution was prepared. The flocculation rate of each group is measured. The results are shown in FIG. 6: addingThe flocculation effect is best when the coagulant aid is used.
2. And optimizing the dosage of the flocculating agent. Use of1%, 2%, 3%, 4% and 5% of flocculating agent is added into the ferric sulfate solution. The flocculant refers to supernatant of fermentation liquor obtained in the step 1 and fermented for 3 days. Wherein, the ratio refers to the volume of the flocculating agent: and (5) the volume of the biogas slurry to be flocculated. The flocculation rate was measured separately. As shown in FIG. 7, 0.4, 0.8, 1.2, 1.6, and 2.0mL of the supernatant of the fermentation broth was used as a flocculant in FIG. 7. FIG. 7 shows that the flocculation effect is best when the dosage of the flocculating agent is 3%.
Example four: effect display of chicken manure biogas slurry from different sources by flocculating alkali producing bacteria
Selecting chicken manure biogas slurry of different batches in chicken manure biogas engineering of Shandong for flocculation test. The test method is the same as the third embodiment, wherein the dosage of ferric sulfate isThe dosage of the flocculant is 3 percent. The results are shown in FIG. 8, where the flocculation rates were 85.79%, 83.87%, 79.97%, 87.68%, 90.32%, and 71.46%, respectively. And the effect of 2 bottles of flocculated chicken manure biogas slurry was arbitrarily selected for display, as shown in fig. 9. Proves that the alcaligenes faecalis has a specific flocculation effect on the chicken manure biogas slurry and is batched with the chicken manure biogas slurryAnd is not related.
Example five: demonstration of effect of flocculation of other biogas slurry by alcaligenes faecalis
And flocculating the cow dung biogas slurry, the pig dung biogas slurry and the vinasse biogas slurry respectively by using supernatant of the alcaligenes faecalis fermentation liquid fermented for 3 days as a flocculating agent and using ferric sulfate as a coagulant aid under the same conditions as in the fourth embodiment. The cow dung biogas slurry is obtained from biogas engineering of a certain dairy farm of Shijiazhuang; the pig manure biogas slurry is taken from biogas engineering of a certain pig farm in a Chengdu dual-flow area; the vinasse biogas slurry is obtained from a vinasse biogas project in a Zunyi sowing area.
The flocculation effects of the cow dung biogas slurry, the pig dung biogas slurry and the vinasse biogas slurry are respectively shown in figures 10, 11 and 12, and the flocculation rates are respectively 3.27%, 5.96% and 2.17%. The results prove that the flocculation effect of the Alcaligenes faecalis on the biogas slurry is mainly reflected on chicken manure biogas slurry, and if the type of the biogas slurry is changed, a good flocculation effect cannot be achieved. The possible reasons are: specific reaction is generated between certain or certain specific substances contained in the chicken manure biogas slurry and the alcaligenes faecalis or metabolites thereof provided by the invention, so that an excellent targeted flocculation effect is achieved.
Example six: effect display of other flocculating agent flocculating chicken manure biogas slurry
1. Under the same conditions AS the fourth example, the flocculant of alcaligenes faecalis (AS28) + ferric sulfate coagulant aid, flocculant AS30 (produced from Brevundimonas diminuta, CGMCC No.17864) + ferric sulfate coagulant aid, flocculant AS33 (produced from Bacillus cereus, CGMCC No.17865) + ferric sulfate coagulant aid, ferric sulfate, polyaluminium chloride and polyacrylamide of the invention were used AS flocculants respectively to flocculate the chicken manure biogas slurry. As shown in FIG. 13, the flocculation rates were 83.87%, 3.27%, 4.51%, 6.44%, 24.31%, and 19.75%, respectively.
2. CGMCC1 No.16044 Alcaligenes faecalis (Alcaligenes faecalis) and CGMCC No.1.924 Alcaligenes faecalis (Alcaligenes faecalis) are purchased from the China center for culture collection and management, and ferric sulfate coagulant aids are added to flocculate the chicken manure biogas slurry under the same conditions as in the fourth embodiment. The results are shown in FIG. 14, where the flocculation rates were 6.37% and 24.15%, respectively. The effect is not ideal.
The tests prove that the AS28 provided by the invention has a targeted flocculation effect on the chicken manure biogas slurry, and the flocculant prepared by other microorganisms or a common chemical flocculant is difficult to achieve a comparable effect.
Sequence listing
<110> institute of biological research of Chengdu of Chinese academy of sciences
<120> Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1470
<212> DNA
<213> Alcaligenes faecalis (Alcaligenes faecalis)
<400> 1
tcagattgaa cgctagcggg atgctttaca catgcaagtc gaactacagc acgcgagagc 60
ttgctctctt ggtggcgagt ggcggacggg tgagtaatat atcggaacgt gcccagtagc 120
gggggataac tactcgaaag agtggctaat accgcatacg ccctacgggg gaaagggggg 180
gatcgcaaga cctctcacta ttggagcggc cgatatcgga ttagctagtt ggtggggtaa 240
aggctcacca aggcaacgat ccgtagctgg tttgagagga cgaccagcca cactgggact 300
gagacacggc ccagactcct acgggaggca gcagtgggga attttggaca atgggggaaa 360
ccctgatcca gccatcccgc gtgtatgatg aaggccttcg ggttgtaaag tacttttggc 420
agagaagaaa aggcatcccc taatacggga tgctgctgac ggtatctgca gaataagcac 480
cggctaacta cgtgccagca gccgcggtaa tacgtagggt gcaagcgtta atcggaatta 540
ctgggcgtaa agcgtgtgta ggcggttcgg aaagaaagat gtgaaatccc agggctcaac 600
cttggaactg catttttaac tgccgagcta gagtatgtca gaggggggta gaattccacg 660
tgtagcagtg aaatgcgtag atatgtggag gaataccgat ggcgaaggca gccccctggg 720
ataatactga cgctcagaca cgaaagcgtg gggagcaaac aggattagat accctggtag 780
tccacgccct aaacgatgtc aactagctgt tggggccgtt aggccttagt agcgcagcta 840
acgcgtgaag ttgaccgcct ggggagtacg gtcgcaagat taaaactcaa aggaattgac 900
ggggacccgc acaagcggtg gatgatgtgg attaattcga tgcaacgcga aaaaccttac 960
ctacccttga catgtctgga atgccgaaga gatttggcag tgctcgcaag agaaccggaa 1020
cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1080
cgagcgcaac ccttgtcatt agttgctacg caagagcact ctaatgagac tgccggtgac 1140
aaaccggagg aaggtgggga tgacgtcaag tcctcatggc ccttatgggt agggcttcac 1200
acgtcataca atggtcggga cagagggtcg ccaacccgcg agggggagcc aatctcagaa 1260
acccgatcgt agtccggatc gcagtctgca actcgactgc gtgaagtcgg aatcgctagt 1320
aatcgcggat cagaatgtcg cggtgaatac gttcccgggt cttgtacaca ccgcccgtca 1380
caccatggga gtgggtttca ccagaagtag gtagcctaac cgtaaggagg gcgcttacca 1440
cggtgggatt catgactggg gtgaagtcgt 1470
Claims (7)
1. Alcaligenes faecalis (A)Alcaligenes faecalis) The application in flocculating chicken manure biogas slurry is characterized in that: the alcaligenes faecalis is preserved in the China general microbiological culture Collection center in 2019, 5 months and 30 days, and the preservation numbers are as follows: CGMCC No. 17863;
the application is as follows: adding an alcaligenes faecalis fermentation product and a coagulant aid into the chicken manure biogas slurry, uniformly mixing, and standing.
2. The use of the alcaligenes faecalis of claim 1 in flocculating chicken manure biogas slurry, characterized in that: ferric sulfate is used as a coagulant aid in the application.
4. The use of the alcaligenes faecalis of claim 1 in flocculating chicken manure biogas slurry, characterized in that: the fermentation product of the alcaligenes faecalis is supernatant of fermentation liquor of the alcaligenes faecalis.
5. The use of the alcaligenes faecalis according to claim 4 in flocculating chicken manure biogas slurry, characterized in that: the fermentation product of the alcaligenes faecalis is taken from fermentation liquor fermented on the 3 rd day.
6. The use of the alcaligenes faecalis of claim 1 in flocculating chicken manure biogas slurry, characterized in that: in the fermentation process of the Alcaligenes faecalis, glucose or sodium acetate is used as a carbon source.
7. The use of the alcaligenes faecalis of claim 1 in flocculating chicken manure biogas slurry, characterized in that: during the fermentation process of the Alcaligenes faecalis, peptone is used as a nitrogen source.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010069156.0A CN111233167B (en) | 2020-01-21 | 2020-01-21 | Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010069156.0A CN111233167B (en) | 2020-01-21 | 2020-01-21 | Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111233167A CN111233167A (en) | 2020-06-05 |
CN111233167B true CN111233167B (en) | 2022-02-08 |
Family
ID=70870440
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010069156.0A Active CN111233167B (en) | 2020-01-21 | 2020-01-21 | Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111233167B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114853206B (en) * | 2022-04-11 | 2023-02-21 | 山东省科学院能源研究所 | Method for promoting coagulation precipitation of anaerobic fermentation biogas slurry of livestock and poultry manure |
CN117821340B (en) * | 2024-02-29 | 2024-05-28 | 中国海洋大学 | Polyacrylamide efficient degrading bacterium and screening method and application thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5544303A (en) * | 1978-09-22 | 1980-03-28 | Hitachi Ltd | Sedimentation property improving method of activated sludge |
CN101392245A (en) * | 2008-07-25 | 2009-03-25 | 东华大学 | Preparation and application of flocculating alcaligenes faecalis fixed pellet |
CN108148790A (en) * | 2018-03-07 | 2018-06-12 | 苏州顶裕节能设备有限公司 | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation |
CN108660178A (en) * | 2018-07-19 | 2018-10-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of high flocculating rate microbial flocculant |
CN108977399A (en) * | 2018-09-13 | 2018-12-11 | 陕西理工大学 | One plant of Bacillus foecalis alkaligenes and its application |
CN109082393A (en) * | 2018-08-15 | 2018-12-25 | 南宁市黄陈生猪养殖场 | One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof |
-
2020
- 2020-01-21 CN CN202010069156.0A patent/CN111233167B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5544303A (en) * | 1978-09-22 | 1980-03-28 | Hitachi Ltd | Sedimentation property improving method of activated sludge |
GB2030557A (en) * | 1978-09-22 | 1980-04-10 | Hitachi Ltd | Method for treating wast water |
CN101392245A (en) * | 2008-07-25 | 2009-03-25 | 东华大学 | Preparation and application of flocculating alcaligenes faecalis fixed pellet |
CN108148790A (en) * | 2018-03-07 | 2018-06-12 | 苏州顶裕节能设备有限公司 | A kind of mixed bacteria, carrier and the method for livestock and poultry biology dejection degradation |
CN108660178A (en) * | 2018-07-19 | 2018-10-16 | 佛山皖阳生物科技有限公司 | A kind of preparation method of high flocculating rate microbial flocculant |
CN109082393A (en) * | 2018-08-15 | 2018-12-25 | 南宁市黄陈生猪养殖场 | One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof |
CN108977399A (en) * | 2018-09-13 | 2018-12-11 | 陕西理工大学 | One plant of Bacillus foecalis alkaligenes and its application |
Non-Patent Citations (1)
Title |
---|
高效絮凝剂产生菌的筛选及絮凝活性研究;张新建等;《微生物学杂志》;20081115;第28卷(第6期);第10-13页 * |
Also Published As
Publication number | Publication date |
---|---|
CN111233167A (en) | 2020-06-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2021077453A1 (en) | Pseudomonas stutzeri, composite microbial inoculum prepared using the pseudomonas stutzeri, and use of the composite microbial inoculum | |
CN111233167B (en) | Alcaligenes faecalis and application thereof in chicken manure biogas slurry flocculation | |
CN108298701A (en) | A kind of fermentation waste water processing method of low biodegradability after Anaerobic Treatment | |
CN111268805B (en) | Bacillus cereus and application thereof in pig manure biogas slurry flocculation | |
CN110218682B (en) | Pseudomycosis bacillus and application thereof in sludge reduction | |
CN107460144B (en) | Aerobic active marine bacterium and preparation method of decolorizing flocculant thereof | |
CN108611291A (en) | One plant of salt tolerance planococcus and its application | |
CN113528374B (en) | Lysis strain, sludge reduction treatment agent and application thereof | |
CN111471625B (en) | Sphingomonas spinosa and application thereof in garbage press filtrate flocculation | |
CN113604407A (en) | Composite microbial algaecide and preparation method and application thereof | |
CN106906157B (en) | Rhodococcus, method for producing flocculant by using same and application of rhodococcus in kelp processing wastewater | |
CN109055259B (en) | Pseudomonas XD-3, application thereof and microbial flocculant | |
CN109609407B (en) | Thermophilic microorganism strain for in-situ sludge reduction and application thereof | |
CN111394260A (en) | Separation and application of microorganisms for treating wastewater | |
CN114292797B (en) | Physarum viscosum and application of microbial flocculant thereof in sewage treatment | |
CN116836847A (en) | Heterotrophic nitrification-aerobic denitrification bacteria and application thereof | |
CN114774322B (en) | Bacillus and method for preparing efficient lead-zinc wastewater flocculant by using same | |
CN107794238B (en) | BFX-01 strain for high yield of biological flocculant and biological flocculant obtained thereby | |
CN102703361A (en) | Facultative anaerobic denitrifying bacterium with self-flocculation capability and purpose thereof | |
CN111233168B (en) | Bioflocculant for specifically flocculating vinasse biogas slurry and preparation method thereof | |
CN111621437B (en) | Otter escherichia coli LM-DK separated from oxidation pond of pig farm and application thereof | |
CN112831434B (en) | Halophilic brevibacterium linum capable of efficiently degrading ammonia nitrogen and application thereof | |
CN112391320B (en) | Strain capable of remarkably improving sedimentation performance of high-density sedimentation tank and application thereof | |
CN111100807B (en) | Sea bacillus ZAA002 strain separated from high-salt hot pickled mustard tuber wastewater and screening method and application thereof | |
CN106929448A (en) | One plant of acinetobacter calcoaceticus CZ1701 bacterial strain and application thereof of degraded different shape nitrogen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |