CN102583772B - Microbial preparation comprising mixed microorganisms (bm-s-1), and a biological treatment method for rivers and lakes and a sludge autodigestion process using the same - Google Patents
Microbial preparation comprising mixed microorganisms (bm-s-1), and a biological treatment method for rivers and lakes and a sludge autodigestion process using the same Download PDFInfo
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- CN102583772B CN102583772B CN201210013307.6A CN201210013307A CN102583772B CN 102583772 B CN102583772 B CN 102583772B CN 201210013307 A CN201210013307 A CN 201210013307A CN 102583772 B CN102583772 B CN 102583772B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The biological treatment method for rivers and lakes using a microbial preparation of the present invention comprises: a step of producing a complex microbial liquid, a step of preparation of mixed materials, a step of inoculation at high temperature, a step of cultivation cultivating inoculated mixed materials, a step of desiccation for drying the mixed materials after the cultivation step to form microbial preparation products, and a step of plunge which plunge the microbial preparation into contaminative river or lake.
Description
Technical field
The present invention relates to bioremediation and the mud self-digestion operation of the microbial preparation that one contains mixing microorganisms (BM-S-1) and the rivers and creeks lake water that utilizes it, the present invention utilizes this microbial preparation to carry out a biological disposal upon at the tributary to pollution and rivers and creeks, lake water under the pretreated situation of physical chemistry, can also realize the recycling of suspended sludge.
Background technology
In general, rivers and creeks is to be polluted by the sanitary sewage of polluted water, industrial wastewater, livestock products waste water etc.
Especially, the sanitary sewage in these source of pollution and livestock products waste water are most of has substantial connection with people's basic living circle.
And, because flourishing highly enriched, the high function hardly-degradable substance increasing of industry makes the pollution of rivers and creeks or lake water more serious.
Aforementioned contaminants is the hard-decomposed waste water that is mixed with various organism and residual chemicals, in worldwide, the main final treatment system of paying attention to " physical chemistry treatment process " that relies on is processed, and utilizes microorganism " biologic treatment process " only bringing into play booster action.
So far, the domestic each department of Korea S are different to the pollution restriction in rivers and creeks, although manage according to very strict benchmark, prior art almost cannot meet permission discharge benchmark.
That is, technology so far cannot reach gratifying processing horizontal in the Pollutant Treatment of aforementioned rivers and creeks, lake water, still more, while these environment being made into applicable environment of carrying out a biological disposal upon, aspect Flow-rate adjustment, suspended sludge and other environment, there are being a lot of difficulties.
The flag that the ecological rivers and creeks of construction lifts in each place autonomous government is that the purification of polluting rivers and creeks has dropped into huge expense, but because pollution speed causes expense to increase faster than recovery speed, its pollution level is also day by day serious, therefore in the urgent need to developing close environmental management countermeasure and the effective treating method of bettering people's living environment.
And, need to set up the huge treatment facility of cost etc. owing to causing rivers and creeks, the urban district stench of resident's resentment and the increase of sewage generating capacity, in the face of the economical load day by day increasing, above-mentioned countermeasures and methods seems to have all the more urgency.
Summary of the invention
[the technical problem to be addressed by invention]
In order to address the above problem, the object of this invention is to provide a kind of rivers and creeks of microbial preparation, bioremediation and mud self-digestion operation of lake water utilized, propose to utilize the microbial preparation that contains mixing microorganisms (BM-S-1) for the water quality of pollution level higher rivers and creeks, urban district, tributary and lake water and the possibility that aquatic environment is carried out a biological disposal upon, not only propose economic cheap application method for existing treatment process, can also recycle the suspended sludge occurring in rivers and creeks, lake water treating processes.
Another object of the present invention is to provide the microbial preparation that one contains mixing microorganisms (BM-S-1), can reduce significantly the expensive expense that the rivers and creeks, high pollution urban district of center, city closed conduit and so on and existing rivers and creeks, lake water occur in processing.
[solving the technical scheme of problem]
In order to achieve the above object, the bioremediation of the rivers and creeks lake water that utilizes microbial preparation of the present invention comprises the following steps: to be numbered mixing microorganisms (BM-S-1) 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight of KCTC 11789BP and to maintain 18 to 30 DEG C of steps of preparing complex microorganism liquor as the microorganism liquid agent of the water (80～95 % by weight) of all the other compositions having mixed to deposit; By be selected from rice husk powder, peat moss powder, rice bran powder and mushroom waste wood dried powder more than one cultivation raw material 70 to 90 % by weight and the preparation process of the mixing raw material that forms of described complex microorganism liquor 10 to 30 % by weight; Under the hot conditions of 65 DEG C～90 DEG C, for the high temperature inoculation step of described mixing raw material 100 weight part inoculation mixing microorganisms (BM-S-1) 0.01 to 1 weight parts; The culturing step that the mixing raw material of high temperature inoculation is cultivated; Make the drying step of microbial preparation commercialization being dried through the mixing raw material of described culturing step; And described microbial preparation is dropped into the rivers and creeks of pollution or the input step of lake water.
Microbial preparation of the present invention is mixed by depositing mixing microorganisms (BM-S-1) 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight and water 80～95 % by weight that are numbered KCTC 11789BP.
Mud self-digestion operation of the present invention comprises the following steps: for water 100 weight parts, drops into the cultivation stoste preparation process of cultivating after microbial preparation 0.1～1 weight part that comprises mixing microorganisms (BM-S-1), molasses 1～10 weight part 2～5 days; After being diluted for the water of 10 times～100 times of above-mentioned cultivation stoste interpolations, drop into molasses 1～10 weight parts for cultivation stoste 100 weight parts after dilution and cultivate the living microorganism liquid preparation process of making living microorganism liquid for 2～5 days; Described living microorganism liquid is dispensed onto to the spreading step in lake water or the rivers and creeks of pollution; Described living microorganism is decomposed to the suspended sludge causing and trap cultivation pool, put into and carry out amphimicrobian decomposition after approximately 1/100～3/100 the living microorganism liquid that is equivalent to sludge volume and the liquefaction sludge liquid preparation process of mud liquefaction; And again drop into rivers and creeks, lake water to induce the mud decrement step of microorganism self-digestion after mixing described liquefaction sludge liquid 10～30 % by weight and described living microorganism 70～90 % by weight.
[beneficial effect]
For being regarded as the hard-decomposed rivers and creeks, urban district of high density, compared with existing physical chemistry treatment process, while being suitable for the bioremediation of rivers and creeks of the present invention, lake water, depend merely on biological treatment and just can obtain excellent processing efficiency, can also reduce significantly the occurrence rate of mud.
Moreover, the present invention can also alleviate the environmental costs burden of local autonomous government, reduces the cost of lake water and rivers and creeks water quality management.
Brief description of the drawings
Fig. 1 is the schema of the bioremediation of the rivers and creeks lake water that utilizes microbial preparation of preferred embodiments of the present invention.
Fig. 2 is the schema of the self-digestion operation of the rivers and creeks lake water mud that utilizes microbial preparation of preferred embodiments of the present invention.
Embodiment
The mixing microorganisms (BM-S-1) that the present invention uses is deposited with Korea Institute of Bioengineering Microbial resources center to deposit office as it on October 20th, 2010 to deposit numbering KCTC11789BP.The isolation identification result of above-mentioned mixing microorganisms, it is by comprising that Prey irrigates Salmonella section (Prevotellaceae_uc_s), lactobacillus genus (Lactobacillus_uc), class Lactobacillus buchneri (Lactobacillusparabuchneri), Lctobacteriaceae (Lactobacillaceae_uc_s), lactobacillus paracasei (Lactobacillus paracasei), Lactobacillus parafarraginis, Lactobacillus camelliae, food cassava Bacterium lacticum (Lactobacillus manihotivorans), Luo Wang acetobacter (Acetobacter lovaniensis), ethanol bacillus (Ethanoligenens_uc), Wei Rong Shi Coccaceae (Veillonellaceae_uc_s), Lactobacillus similis, Lactobacillus harbinensis, the formations such as Rhodospirillales (Rhodospirillales_uc_s) and other kind more than 130 bacterium miscellaneous and yeast.
The invention provides using above-mentioned deposit the mixing microorganisms (BM-S-1) that is numbered KCTC 11789BP as the microbial preparation of effective constituent, utilize its to process bioremediation and the mud self-digestion operation of rivers and creeks, lake water.
At this, preferably, rivers and creeks comprises rivers and creeks, urban district, closed conduit, general rivers and creeks and huge rivers and creeks, and lake water comprises that agricultural is used and lake, general waterside.
Describe content of the present invention below in detail.
The sepn process of the mixing microorganisms (BM-S-1) that the invention described above is used is as follows.Take to mix with the substratum (rice bran, rice husk, sawdust, eggshell, shell, peat moss) that is ground into 80～120 sieve meshes after soil test portion (bamboo leaf-humidified soil, ruminant stomach be digest, deciduous tree leaf-humidified soil not) and form after water activity 40～60% the soil incubation of half tree shade state 90 days.The substratum that has mixed rice bran 30% weight, rice husk 20% weight, peat moss 20% weight, sawdust 30% weight is adjusted into after water activity 60%, here with 0.01% inoculation of overall broth weight through the test portion of culturing process and the temperature inoculation of 65～90 DEG C after 4 hours, it is the powder-mixed microorganism (BM-S-1) below 8% that moisture concentration is made in fermentation 3 weeks.
Utilize the microbial species bacterium of separating like this to prepare microbial preparation of the present invention with following method.
First, be mixed and made into complex microorganism liquor using depositing mixing microorganisms (BM-S-1) 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight and the water (80～95 % by weight) as all the other compositions that are numbered KCTC 11789BP.Then, described complex microorganism liquor is utilized to 30 to 80ml with the interval of 4～8 hours under the situation that maintains 18 to 30 DEG C of temperature
3the process of the air aeration 1 to 2 hour of/min repeats 4 times every day, cultivates 7 to 10 days.
Now, according to the inventor's result of study, the conditions such as aeration are optimums, although the technology personage of this area can slightly do to change to above-mentioned culture condition, should know that this variation can not depart from interest field of the present invention.
Then, preparation is selected from more than one cultivation raw material 70 to 90 % by weight and the mixing raw material that forms of above-mentioned complex microorganism liquor 10 to 30 % by weight in rice husk powder, peat moss powder, rice bran powder and mushroom waste wood dried powder, then carries out the high temperature inoculation step for above-mentioned mixing raw material 100 weight parts inoculation mixing microorganisms (BM-S-1) 0.01 to 1 weight parts.
At this, cultivation raw material can use and easily obtain and lower-cost rice husk, agricultural and sideline product or minor forest products etc.
Described cultivation raw material uses after can using separately above-mentioned rice husk, agricultural and sideline product, minor forest products or combination, preferably, in advance above-mentioned cultivation raw material powder is broken into 150 sieve meshes (mesh) left and right with Powdered use.
Raw material described mixing and after pulverizing is put into after rotating and culturing base, utilize complex microorganism liquor to regulate moisture in order to maintain the environment of applicable microorganism fermentation, then inoculation contains the mixing microorganisms (BM-S-1) that bacillus (bacillus) belongs to bacterial strain or milk-acid bacteria.Above-mentioned mixing microorganisms (BM-S-1) does not have artificial to filter that ground directly takes to have season, after the microbial of the multifarious soil of environment, make Powdered on natural goods substratum after the environmental adaptation process of 9 months is removed hazardous property.
Especially the cultivation raw material of in an embodiment of the invention, having inoculated above-mentioned mixing microorganisms (BM-S-1) stirs and 6 to 8 hours Di has carried out high temperature inoculation with 20 to 200rpm/min speed the temperature of 65 DEG C to 90 DEG C.
Consider that general microbial inoculant carries out 20 DEG C to 40 DEG C scopes, known microbial preparation of the present invention carries out under hot conditions.The reason that uses aforementioned inoculation temp is in order to suppress unnecessary microbial growth and to maintain microbic activity of the present invention.
After high temperature inoculation step, carry out culturing step the mixing raw material of above-mentioned inoculation is cultivated 10～15 days, then carry out drying step in order to allow microbial preparation commercialization.
Drying step can use known all drying meanss and be not particularly limited in the scope of microbial preparation thermally denature approximately.An embodiment of the invention are carried out seasoning 8 hours with the speed of 170～180rpm/min in rotating and culturing base.
And the present invention does not get rid of microorganism is directly added to processing environment, the sustainable form that maintains microorganism active is added comparatively effective with the form that is adsorbed on carrier.Although can be written into by the simple absorption of carrier surface while being written into microorganism, be written into comparatively favourable on fixation support.That is, while utilizing fixation support, can improve microbic activity and shorten the resolving time, therefore this form is better.
The form of adding after microorganism embedded immobilization can be made to the stable also sensitization of activity of microorganism.The specific examples of preceding method has had known the whole bag of tricks.According to the water treatment method of the invention described above, utilize microbial preparation of the present invention the rivers and creeks of high-pollution load, lake water specifically can be reduced to the level below benchmark value.
As long as being written into the material that drops into lake water, rivers and creeks after mentioned microorganism, microorganism is written into and can uses known any material with carrier, but be efficiently written into viewpoint with microorganism, preferably, use can make microorganism strong adsorption at the material of carrier surface, the porous material that can make microorganism invade to maintain ability in fine voids to improve keeping, micropartical aggegation and be able to that actual raising is adsorbed or the material of adsorption surface area.Specifically, can be the polyose of Mierocrystalline cellulose, glucosan (dextran), agarose (Agarose) and so on; The nonactive protein of collagen, gel (gelatin), albumin and so on; The synthetic macromolecular compound of ion exchange resin, polyvinyl chloride and so on; The inorganics of pottery or porous glass and so on; The natural carbon hydride of agar (agar-agar), Lalgine (alginic acid), carrageenin (carrageenan) and so on; Or cellulose acetate, polyacrylamide, polyvinyl alcohol, epoxy resin, thermosetting resin, polyester, polystyrene, urethane and so on can be used as embedding carrier and macromolecular compound etc.And, also can utilize the material being formed by xylogen (lignin), starch, chitin (chitin), oligochitosan (Chitosan), filter paper, wood chip etc.Microorganism be written into immobilization in, especially contain the form that is written into of microorganism with carrier substance, embedded immobilization is better.The preferred shape of carrier is that summary is spherical, summary cubic, summary are rectangular-shaped, round shape or pipe (tube) shape, wherein spherical with the summary easily made or can increase the intimate rectangular-shaped better of specific area.The making method of carrier can be used existing any method.For example, the mixing solutions of microorganism and carrier substance (or its presoma) is splashed into the drop making in non-insoluble liquid in liquid solidifies and preparation microorganism is written into the method with the dispersion of carrier particle, the mixing solutions of microorganism and carrier substance (or its presoma) with low temperature, add after the methods such as peptizing agent or solidifying agent are solidified cured body is cut into the method for making the rectangular parallelepiped particle that is written into microorganism after suitable size, the mixing solutions of microorganism and carrier substance (or its presoma) is extruded and in nozzle injects non-insoluble liquid in liquid, solidified and obtain microorganism and be written into the method that is cut into the round shape particle of suitable size after the cured article with carrier, or now extrusion moulding nozzle uses annular and the microorganism that obtains circular (tubulose) is written into the method with carrier particle.The feature of embedded immobilization method is thalline can be maintained to high density state, and therefore improving processing efficiency also can be bacteria immobilization slower propagation.And the patience that the condition such as Ph, temperature is changed greatly and can bear higher load condition.As long as embedded immobilization method can be enclosed microorganism and be maintained microbic activity in carrier, maintain high physical strength and be enough to long-time use, just will not limit, it comprises following method: acrylamide method, agar (agar-agar)-acrylamide method, PVA-borate method, PVA-cold method, ray hardening resin method, acrylic acid series synthetic macromolecule resin method, sodium polyacrylate method, sodium alginate method, K-carrageenin (carrageenan) method etc.As another embedding immobilization method, can on active carbon particle, give immobilization.As another embedding immobilization method, specified microorganisms can be immobilized on carbon cloth.It is the example as the carbon fiber of carrier use that coal tar pitch high-temperature fusion is radiated to the fiber that gives obtaining after insoluble carbonization.Preferably, the thick 0.3～6.0mm, the unit weight 20～300g/m that use the carbon fiber of diameter 1～30 μ m to form
2carbon fiber.And microbial preparation of the present invention can optionally add various additives, such as mineral (flocculation agent), Lalgine and salt thereof, organic acid, protective colloid tackifier, preparation etc. for forming.The addition means of the microbial preparation of making according to mode shown in above, just can take any method as long as can be evenly dispersed in treating pond.For example, purify or stirrer etc. stirs and microorganism is directly manually dropped into from accepting container by draining after can dropping into air in the waste water for the treatment of pond.The whole volume in complex processing pond of processing lake water, rivers and creeks, in general, was adjusted into the waste water residence time of complex processing pond entirety about 2 days to 7 days according to wastewater flow rate and different from the residence time.Preferably, the residence time is adjusted to about 3 days to 5 days.Although the treating pond quantity forming is restriction not, from efficiency and installation cost viewpoint, better with 3 to 5 ponds.After the factors such as the COD value before and after processing and can measure pH, DO (dissolved oxygen) when microbial preparation, processing, manage.PH is 2.8～9.5, with 3.5～8.5 better, also can select narrower span of management according to the character of institute's suitable environment.DO is 3.0mg/L～12.0mg/L, better with 4.0mg/L～7.0mg/L.PH can control by the mode of adding acid or alkali, and DO drops into air and controls by the mode that regulates draining purge amount in sewage.Although the concentration determination of specific compound can be used direct quantitative, in managerial practical methods to use COD corresponding to the value of concentration.While measuring COD, preferably, measure initial polluted water treating pond entrance and the final concentration of processing water out both sides.The nutrition source of polluted water treating pond can drop into carbon source, nitrogenous source or the organotrophy source, the inorganic nutrients source that are applicable to microorganism fertility.Drop into polyprotein peptone (Polyeptone), yeast concentrated solution, meat concentrated solution, molasses etc. as organotrophy source, drop into various phosphoric acid salt, magnesium salts etc. as inorganic nutrients source, the addition in organotrophy source is 0.001～0.5 % by weight of wastewater flow rate, better with 0.01～0.05 % by weight, the addition in inorganic nutrients source is 0.1～1 % by weight left and right in organotrophy source.This amount is not determinate, can suitably select according to the state of microorganism suitable environment.
Then, the present invention is numbered mixing microorganisms (BM-S-1) 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight of KCTC 11789BP and also belongs to interest field of the present invention as the microbial preparation forming after the water (80～95 % by weight) of all the other compositions having mixed to deposit, and first gives and illustrating at this.At this, preferably, microbial preparation is Powdered, or described microbial preparation is living microorganism liquid form.
Rivers and creeks, the lake water mud self-digestion operation of utilizing the microbial preparation that relies on above-mentioned formation to obtain to carry out are carried out as follows.First, drop into after microbial preparation 0.1～1 weight part, molasses 1～10 weight part that has utilized mixing microorganisms (BM-S-1) for water 100 weight parts, cultivate and within 2～5 days, make cultivation stoste at normal temperature (10～30 DEG C).
Then,, after the water of 10 times～100 times of above-mentioned cultivation stoste interpolations is diluted, drop into molasses 1～10 weight parts and cultivate and within 2～5 days, make living microorganism liquid at normal temperature (10～30 DEG C) for cultivation stoste 100 weight parts after dilution.
Then, living microorganism is dispensed onto to lake water or the rivers and creeks of pollution, preferably, above-mentioned lake water is comparatively applicable with rivers and creeks, urban district, closed conduit, general rivers and creeks, and above-mentioned lake water is comparatively applicable with lake water and lake, general waterside with agricultural.
Then, above-mentioned living microorganism is decomposed to the suspended sludge causing and trap cultivation pool, carry out amphimicrobian decomposition and mud is liquefied after putting into approximately 1/100～3/100 the living microorganism liquid that is equivalent to sludge volume.
Then, after mixed liquefied sludge liquid 10～30 % by weight and living microorganism 70～90 % by weight, again drop into rivers and creeks, lake water, repeat said process and realize the self-digestion of mud.
Describe content of the present invention in detail below in conjunction with embodiment and experimental example.But they just describe the present invention in detail and can not limit interest field of the present invention.
the cultivation of embodiment 1 mixing microorganisms (BM-S-1)
The soil test portion (peat moss, bamboo leaf-humidified soil, ruminant stomach be digest, deciduous tree leaf-humidified soil not) that thermal treatment contains microorganism at 90 DEG C is even at mortar mill after 40 minutes, after then taking out test portion 1g and suspending on 0.85%NaCl 9mL, is diluted to 10
0to 10
-7.Each diluted suspension 100 μ L, in the upper line of TSA, BL, BBL substratum (DIFCO company) (streak), are cultivated and turn out mixing microorganisms (BM-S-1) at 28 DEG C.
Learn from the result of utilizing tetra-sodium order-checking (pyrosequencing) to separate above-mentioned mixing microorganisms (BM-S-1), it is by comprising that Prey irrigates Salmonella section (Prevotellaceae_uc_s), lactobacillus genus (Lactobacillus_uc), class Lactobacillus buchneri (Lactobacillus parabuchneri), Lctobacteriaceae (Lactobacillaceae_uc_s), lactobacillus paracasei (Lactobacillus paracasei), Lactobacillus parafarraginis, Lactobacillus camelliae, food cassava Bacterium lacticum (Lactobacillus manihotivorans), Luo Wang acetobacter (Acetobacter lovaniensis), ethanol bacillus (Ethanoligenens_uc), Wei Rong Shi Coccaceae (Veillonellaceae_uc_s), Lactobacillus similis, Lactobacillus harbinensis and Rhodospirillales (Rhodospirillales_uc_s) form on the interior bacterium miscellaneous of kind more than 130 (reference table 1) and yeast (Candida boidinii (Candida boidinii)).
[table 1]
| Plant name | Ratio (%) |
| Prey is irrigated Salmonella section (Prevotellaceae_uc_s) | 22.2 |
| Lactobacillus genus (Lactobacillus_uc) | 17.7 |
| Class Lactobacillus buchneri (Lactobacillus parabuchneri) | 6.9 |
| Lctobacteriaceae (Lactobacillaceae_uc_s) | 6.5 |
| Lactobacillus paracasei (Lactobacillus paracasei) | 5.8 |
| Lactobacillus parafarraginis | 4.3 |
| Lactobacillus camelliae | 3.0 |
| Food cassava Bacterium lacticum (Lactobacillus manihotivorans) | 2.4 |
| Luo Wang acetobacter (Acetobacter lovaniensis) | 2.3 |
| Lactobacillus collinoides (Lactobacillus collinoides) | 2.2 |
| Lactobacillus vini | 2.0 |
| Lactobacillus hilgardii (Lactobacillus hilgardii) | 1.8 |
| Lactobacillus pentosus (Lactobacillus pentosus) | 1.7 |
| Lactobacillus rapi | 1.5 |
| Lactobacillus pantheris | 1.3 |
| Ethanol bacillus (Ethanoligenens_uc) | 1.2 |
| Wei Rong Shi Coccaceae (Veillonellaceae_uc_s) | 1.2 |
| Lactobacillus similis | 1.2 |
| Lactobacillus harbinensis | 1.0 |
| Rhodospirillales (Rhodospirillales_uc_s) | 0.5 |
| Other | 13.8 |
| Altogether | 100.0 |
* be altogether 6801 clone analytical resultss; * uc_s represents unfiled species (unclassified species).
At this, lactobacillus (Lactobacillus sp.) converts lactic acid (lactic acid) to lactose (lactose) and other sugar (sugars) and makes surrounding environment become acidic conditions or Hydrogen Peroxide and suppress the harmful microbe growths such as pathogenic microbes.Aspect environment, mainly inhabit in the plant residue of decomposition, be also present in the intestines of the mankind or animal and show probiotic bacterium (probiotic) activity.Be divided into Acetobacteraceae (Acetobacteraceae) and Rhodospirillaceae (Rhodospirillaceae) at Rhodospirillales (Rhodospirillales), Acetobacteraceae (Acetobacteraceae) comprises the Luo Wang acetobacter shown in table 1 (Acetobacter lovaniensis), this kind has the aerobic that can form acetic acid from alcohol (Alcohol), perches at the position of the root of each kind of plant (sugarcane, Ipomoea batatas, coffee, tea, banana etc.), stem, fruit, leaf and so on.And, Rhodospirillaceae (Rhodospirillaceae) comprises purple nonsulfur bacteria and green without thiobacterium, and they utilize various organic acids or the ethanol (ethanol) that lactobacillus (Lactobacillus sp.), bacillus aceticus (Acetobacter sp.) and other Anaerobic Bacteria (Ethanoligenens sp.) of being present in this microbial preparation generate grow up or pass through photosynthesis fixation of C O
2and play an important role in by the useless water purification of Organic pollutants.The fertile Salmonella section (Prevotellaceae) of Prey that occupies many density in this preparation is mainly present in the intestines of normal warm-blooded animal (mankind, animal etc.) and sugar can be converted to succinic acid (succinic acid) or acetic acid, judges that it contributes to organic fast decoupled.And, advantage species (dominant species) yeast of separating from this research is accredited as Candida boidinii (Candida boidinii), by inference, it can generate the physiologically active substance of VITAMIN or amino acid and so on and growth and the organic substance decomposing of above-mentioned mixing microorganisms (BM-S-1) are contributed.
embodiment 2 utilizes the pollution rivers and creeks processing of microbial preparation
The mixing microorganisms of above-mentioned cultivation (BM-S-1) 0.02kg, rice husk powder 10kg, peat moss powder 4kg, mushroom waste wood powder 2kg, molasses 3kg and the water as its surplus are mixed to form after the weight of 100kg, assign to the interval 60L of 6 hours with 30
3the process of 30 points of the air aerations 1 hour of/min repeats 4 times every day, cultivates 10 days and makes complex microorganism liquor.The total count of above-mentioned complex microorganism liquor is 2.6 × 10
9cfu/g.In addition, the rice husk 45kg, peat moss powder 40kg, rice bran 5kg, the mushroom waste wood 10kg that pulverize are in advance put into after the rotating and culturing base with mixing functions, on the raw material mixing, put above-mentioned complex microorganism liquor 20kg into and adjust to water activity concentration 40～60%, then with the 0.05 weight part inoculation mixing microorganisms (BM-S-1) with respect to cultivating raw material entirety 100 weight parts.Allow the cultivation raw material of inoculation carry out high temperature inoculation 8 hours with 180rpm/min rotation and at 70 DEG C of substratum internal temperatures.Then the mixing raw material of high temperature inoculation is not heated, do not cultivated rotatably 15 days in rotating and culturing base.Then, in rotating and culturing base, after 180rpm/min rotation is dried for 8 hours, do not make moisture concentration as the Powdered microbial preparation below 8% heatedly, then process polluting rivers and creeks.
embodiment 3 utilizes the mud self-digestion operation of microbial preparation
In city of Pusan, newly flat long woods river middle and lower reaches 1.2km section has been suitable for the microbial preparation of the embodiment of the present invention.1 ton of tank is put into and is stirred after above-mentioned Powdered microbial preparation 5kg and molasses 30kg and cultivate after 72 hours at normal temperature, 1 ton of the content of cultivation is poured into the water of 49 tons and dilute and drop into and carry out sensitization after molasses 100kg and within 72 hours, make living microorganism liquid.
Safety inventory (safety inventory) at 50 tons of tanks is 15 ton hours, again makes 50 tons of above-mentioned living microorganism liquid and uses afterwards.The living microorganism liquid of 50 ton-miles gives drop according to the place of one day mode closed conduit end before Borim primary school of 5 tons and drops into, 5 tons of same living microorganism liquid are by tank car (tank lorry) shipment and loose input of closed conduit peristome 4 punishment, the sewage influx of every day is decided to be to 50,000 tons.Do not carry out any chemical treating process, its result, the stench occurring on rivers and creeks reduces significantly, after the mud occurring is sucked by liquefaction decomposing pool in addition, induction liquefaction is decomposed 48 hours, after mixing with living microorganism liquid with the ratios of 2: 8, again drop into, repeat the method and realized the object of mud decrement.Stench reduces, and if following table 2 mixing microorganisms (BM-S-1) induced activity microorganism liquid is to as shown in the purification result of the long woods of new adit river, city of Pusan middle and lower reaches, learns that from the measured value of BOD, COD and T-N etc. quality of river water has obtained significantly improving.
[table 2]
| Classification | July | August | September | October | November | December | Processing efficiency (%) |
| pH | 7.5 | 7.7 | 7.2 | 7.7 | 7.5 | 7.6 | - |
| COD | 215 | 220 | 218 | 140 | 80 | 23 | 89.3 |
| SS(mg/L) | 338 | 450 | 380 | 210 | 151 | 38 | 88.8 |
| NH 3-N(mg/L) | 85 | 82 | 69 | 31 | 12 | 8 | 90.6 |
| NO 2-N(mg/L) | 35 | 40 | 27 | 20 | 8 | 2 | 94.3 |
| NO 3-N(mg/L) | 16 | 93 | 43 | 31 | 23 | 8 | 50.0 |
| TN(mg/L) | 136 | 215 | 139 | 82 | 43 | 18 | 86.8 |
* be the processing efficiency data with respect to July to December.
Be illustrated with reference to preferred embodiment of the present invention above, but be familiar with, in the field of the invention that this study group of the art can record in following claims, this technology is carried out to various development.
Claims (4)
1. a bioremediation of utilizing rivers and creeks and the lake water of microbial preparation, comprises the following steps:
Be numbered mixing microorganisms BM-S-1 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight of KCTC11789BP and maintain 18 to 30 DEG C of steps of preparing complex microorganism liquor as the microorganism liquid agent of the water of all the other compositions using having mixed to deposit;
By be selected from rice husk powder, peat moss powder, rice bran powder and mushroom waste wood dried powder more than one cultivation raw material 70 to 90 % by weight and the preparation process of the mixing raw material that forms of described complex microorganism liquor 10 to 30 % by weight;
Under the hot conditions of 65 DEG C～90 DEG C, for the high temperature inoculation step of described mixing raw material 100 weight part inoculation mixing microorganisms BM-S-1 0.01 to 1 weight parts;
The culturing step that the mixing raw material of high temperature inoculation is cultivated;
Make the drying step of microbial preparation commercialization being dried through the mixing raw material of described culturing step; And
Described microbial preparation is dropped into the rivers and creeks of pollution or the input step of lake water.
2. a microbial preparation, is characterized in that:
Be mixed by depositing mixing microorganisms BM-S-1 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight and water 80～95 % by weight that are numbered KCTC11789BP.
3. a mud self-digestion operation of utilizing microbial preparation, comprises the following steps:
For water 100 weight parts, drop into and comprise the cultivation stoste preparation process of cultivating after microbial preparation 0.1～1 weight part, molasses 1～10 weight part of depositing the mixing microorganisms BM-S-1 that is numbered KCTC11789BP 2～5 days;
After being diluted for the water of 10 times～100 times of above-mentioned cultivation stoste interpolations, drop into molasses 1～10 weight parts for cultivation stoste 100 weight parts after dilution and cultivate the living microorganism liquid preparation process of making living microorganism liquid for 2～5 days;
Described living microorganism liquid is dispensed onto to the spreading step in lake water or the rivers and creeks of pollution;
Living microorganism in described living microorganism liquid is decomposed to the suspended sludge causing and trap cultivation pool, put into and carry out amphimicrobian decomposition after 1/100～3/100 the living microorganism liquid that is equivalent to sludge volume and the liquefaction sludge liquid preparation process of mud liquefaction;
After mixing above-mentioned liquefaction sludge liquid 10～30 % by weight and above-mentioned living microorganism liquid 70～90 % by weight, again drop into rivers and creeks, lake water to induce the mud decrement step of microorganism self-digestion.
4. mud self-digestion operation according to claim 3, is characterized in that:
The microbial preparation that contains above-mentioned mixing microorganisms BM-S-1 is mixed by depositing mixing microorganisms BM-S-1 0.01 to 1 % by weight, rice husk powder 1 to 10 % by weight, peat moss powder 1 to 10 % by weight, mushroom waste wood 1 to 5 % by weight, molasses 1 to 10 % by weight and water 80～95 % by weight that are numbered KCTC11789BP.
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| KR1020110124372A KR101122766B1 (en) | 2011-01-14 | 2011-11-25 | The probiotics containing mixed strains of bm-s-1 and methods for biological treatment of polluted streams and lakes using the probiotics and process for self-digestion of sludge |
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| KR101303608B1 (en) | 2012-05-08 | 2013-09-11 | 주식회사 비엠 | The probiotics containing mixed strains of bm-s-1 and biological treatment method and self-digestion process of marine dredged soil or coast sludge using the probiotics |
| KR101259416B1 (en) * | 2013-01-31 | 2013-04-30 | 주식회사 비엠인터내셔날 | The manufacturing method of probiotics containing mixed strains bm-s-1 for soil contamination purification or soil environmental improvement and manufactured probiotics thereof |
| CN103449592A (en) * | 2013-09-02 | 2013-12-18 | 苏州富奇诺水治理设备有限公司 | Environment-friendly wastewater treatment agent |
| KR101721185B1 (en) | 2015-09-01 | 2017-03-29 | 농업회사법인(주)이지엠앤알 | Manufacturing Method of Microbial Agent for annnihilation of Organic Sludge and Microbial Agent for annihilation of Organic Sludge manufactured by the same |
| KR20210101710A (en) | 2020-02-10 | 2021-08-19 | 농업회사법인 주식회사 미드숨비 | The manufacturing method of nano multi-complex fermented microorganism for soil contamination purification or soil environmental improvement and manufactured nano multi-complex fermented macroorganism thereof |
| KR102359725B1 (en) * | 2020-03-24 | 2022-02-08 | 주식회사 클린이노베이션 | Natural antibacterial germicide |
| CN112441664A (en) * | 2020-11-06 | 2021-03-05 | 广州小众环保科技有限公司 | Composite carbon source sewage treatment agent and preparation method thereof |
| CN112978936A (en) * | 2021-02-05 | 2021-06-18 | 王德利 | Bioactive composite fermentation liquor for purifying water quality by using microorganisms |
| CN113136351B (en) * | 2021-04-25 | 2023-04-07 | 重庆融极浩瀚生物技术有限公司 | Composite microbial flocculant and preparation method and application thereof |
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