CN103190538A - Aflatoxin B1 degradation agent and application thereof - Google Patents
Aflatoxin B1 degradation agent and application thereof Download PDFInfo
- Publication number
- CN103190538A CN103190538A CN2013100671904A CN201310067190A CN103190538A CN 103190538 A CN103190538 A CN 103190538A CN 2013100671904 A CN2013100671904 A CN 2013100671904A CN 201310067190 A CN201310067190 A CN 201310067190A CN 103190538 A CN103190538 A CN 103190538A
- Authority
- CN
- China
- Prior art keywords
- afb
- afb1
- composition
- degradation agent
- component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 31
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 title claims abstract description 20
- 229930020125 aflatoxin-B1 Natural products 0.000 title claims abstract description 20
- 230000015556 catabolic process Effects 0.000 title claims abstract description 19
- 239000003795 chemical substances by application Substances 0.000 title abstract description 10
- 239000002115 aflatoxin B1 Substances 0.000 title abstract description 4
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 101100449517 Arabidopsis thaliana GRH1 gene Proteins 0.000 claims abstract description 16
- 101100434479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) AFB1 gene Proteins 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 8
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 7
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 6
- 230000004151 fermentation Effects 0.000 claims abstract description 6
- 239000007787 solid Substances 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 102000038379 digestive enzymes Human genes 0.000 claims description 12
- 108091007734 digestive enzymes Proteins 0.000 claims description 12
- 241000287828 Gallus gallus Species 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 241000235648 Pichia Species 0.000 claims description 7
- 235000014121 butter Nutrition 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 7
- 238000012216 screening Methods 0.000 claims description 5
- 238000012797 qualification Methods 0.000 claims description 3
- 230000002631 hypothermal effect Effects 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 244000286779 Hansenula anomala Species 0.000 abstract description 9
- 241000233866 Fungi Species 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 5
- 230000000593 degrading effect Effects 0.000 abstract description 5
- 230000002068 genetic effect Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000014683 Hansenula anomala Nutrition 0.000 abstract description 2
- 244000199866 Lactobacillus casei Species 0.000 abstract description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 abstract description 2
- 229940017800 lactobacillus casei Drugs 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 230000003950 pathogenic mechanism Effects 0.000 abstract 1
- 231100000678 Mycotoxin Toxicity 0.000 description 9
- 239000002636 mycotoxin Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 5
- 239000003463 adsorbent Substances 0.000 description 4
- 238000001784 detoxification Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000004886 process control Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000016788 immune system process Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000026676 system process Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses an aflatoxin B1 degradation agent comprising a component a and a component b with a combination ratio of 2 : 3, wherein the component a is prepared by individually culturing lactobacillus casei CGMCC1.62, Bacillus subtilis CGMCC1.504, and Hansenula anomala CGMCC2.881 for 48 hours, mixing with a ratio of 2 : 1 : 1, adding a carrier, and drying under a low temperature; and the component b is prepared by using AFB1 as a carbon source and energy to screen AFB1 degrading bacteria strains, a 26S rDNA identification result showing that a fungi: Aspergillus oryzae is screened to be capable of degrading the AFB1 and the Aspergillus oryzae being with an access No. CGMCC5817 in the China microbiological culture collection center; performing a solid fermentation culture for the AFB1 degrading fungi, extracting a crude enzyme solution of AFB1 degrading enzymes, adding a carrier, and drying under the low temperature. The aflatoxin B1 degradation agent establishes foundations for an application thereof in animal production, pathogenic mechanism researching of AFB1 on a genetic level, and measures taken to mitigate the harm.
Description
Technical field
The invention belongs to poultry cultivating technology, be specifically related to a kind of AFB1 degradation agent and application thereof.
Background technology
Whole world every year gets more than 1/4 because the cereal crops of mycotoxin contamination account for total output of grain, and the generation of the mould fungus inhibition toxin of taking measures is also carried out detoxification treatment to grain and the feed that pollutes, and is to reduce the loss and watch for animals and an urgent demand of human health.Widely used poison-removing method is by the method for adding adsorbent contaminated grain and forage crop to be carried out detoxification treatment in animal produces at present.But mostly this method is the chelating of adsorbent and mycotoxin, though effective absorbing mycotoxin, mycotoxin very easily dissociated and toxicity still exists from adsorbent when but this condition that simply is adsorbed on changed, simultaneously adsorbent in also can adsorption feed some nutriments and influence its utilization rate.Therefore take more effective and safe method that mycotoxin is carried out detoxification treatment and just become the key of dealing with problems.Adopt the microbial degradation mycotoxin, because it has efficient height, noresidue, advantage such as with low cost and easy and simple to handle has caused researcher's extensive concern, especially utilize the enzyme of microorganism or its generation degrading mold toxin more to be become the focus of present research for non-toxic products.
Be the effective ways that enlarge feed resource, reduce loss to carried out detoxification treatment by the cereal crops of mycotoxin contamination and feed, and if can take measures to suppress the growth of mould in the raw material storage, then can reduce mycotoxin from the source and produce.Therefore can consider to utilize the competition effect between the microorganism, the microorganism of mould fungus inhibition growth or the growth that its metabolite adds mould fungus inhibition in the feedstuff in proportion to will be had, and then the generation of inhibition toxin, thereby reach the purpose that prevention livestock and poultry mycotoxin is poisoned.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of AFB1 degradation agent, to AFB
1Have on the basis of the probio of degradation and digestive enzyme, with probio and AFB
1The digestive enzyme fit applications is observed it to AFB in the broiler fodder in broiler chicken is produced
1Detoxifying effect and explore its detoxifcation mechanism, for its application in animal produces provides foundation.
Its technical scheme is as follows:
A kind of AFB
1Degradation agent comprises composition a and composition b, and its portfolio ratio is 2: 3, wherein
The preparation method of described composition a is: butter bacillus CGMCC1.62, bacillus subtilis CGMCC1.504, unusual Hansenula yeast CGMCC2.881, cultivate 48h separately after, mix the back with 2: 1: 1 ratios and load the body hypothermia drying.
The preparation method of described composition b is: with AFB
1Carry out AFB as carbon source and the energy
1The screening of degradation bacteria strains, the qualification result demonstration through 26S rDNA screens degraded AFB
1Fungi be aspergillus oryzae, the accession designation number at Chinese microorganism strain preservation center is CGMCC5817, to the degraded AFB
1Fungi carry out solid fermentation and cultivate, extract AFB
1The digestive enzyme crude enzyme liquid, low temperature drying behind the adding carrier.
Composition a and composition b, in making the AFB1 degradation agent after the ratio mixing in 2: 3, wherein the content of butter bacillus, bacillus subtilis and unusual three kinds of bacterium of Hansenula yeast is respectively 2 * 10
9Individual/g, 1 * 10
9Individual/g and 1 * 10
9Individual/g, AFB
1The digestive enzyme vigor is 135U/g.AFB
1The digestive enzyme vigor is defined as: per minute degraded 1ng AFB under 37 ℃ condition
1It is enzyme unit alive.
Invent described AFB
1The application of degradation agent in broiler chicken is produced.
Beneficial effect of the present invention:
The present invention obtains Aspergillus flavus growth and AFB by screening
1Degradation effect is bacterial strain preferably, and to AFB
1Digestive enzyme carries out separation and purification, afterwards with probio and AFB
1The digestive enzyme fit applications is observed it to AFB in the broiler fodder in broiler chicken is produced
1Detoxifying effect, use genetic chip that related gene expression amount in the liver is measured at last, be AFB
1The application of degraded in animal produces, and study AFB from gene level
1Mechanism of causing a disease and take measures to alleviate its harm and lay a good foundation.
Description of drawings
Fig. 1 is probio combination and AFB
1Digestive enzyme crude enzyme liquid compatibility is to AFB
1The degradation schematic diagram, what A, B, C represented is the significance of difference in the statistical analysis, capitalization different table differential different significantly (P＜0.05) wherein has capitalization identical table differential different not significantly (P＞0.05);
The specific embodiment
Below in conjunction with the accompanying drawing specific embodiment method of the present invention is done explanation in further detail.
1, test strain and material
Test used butter bacillus (Lactobacillus casei), bacillus subtilis (Bacillus subtilis), unusual Hansenula yeast (Hansenula anomala) all available from Chinese common micro-organisms culture presevation administrative center, and effluent south agriculture university's biotechnology and the preservation of Animal nutrition research department.AFB1 is available from Sigma company, and it is measured and adopts the German RIDASCREEN Aflatoxin B1 of company 30/15 ELISA measuring reagent kit.
2, the preparation of single probio
(1) preparation of butter bacillus nutrient solution: press the inoculum concentration of 4% (v/v) at MRS inoculation of medium butter bacillus, place 37 ℃ of static cultivations of constant temperature, gather in the crops when waiting to cultivate 48h.The content of regulating bacterium is 1 * 10
9CFU/mL.
(2) preparation of bacillus subtilis bacteria culture fluid: press the inoculum concentration of 4% (v/v) LB inoculation of medium bacillus subtilis, place 37 ℃ of constant-temperature shaking culture (150 rev/mins), gather in the crops when waiting to cultivate 48h.The content of regulating bacterium is 1 * 10
9CFU/mL.
(3) preparation of unusual Hansenula yeast nutrient solution: press the inoculum concentration of 4% (v/v) at the unusual Hansenula yeast of LB inoculation of medium, place 30 ℃ of constant-temperature shaking culture (150 rev/mins) to cultivate, gather in the crops when waiting to cultivate 48h.The content of regulating bacterium is 1 * 10
9CFU/mL.
Wherein, the composition of MRS culture medium: tryptone 10g, beef peptone 10g, yeast 5g, glucose 20g, K
2HPO
42g, sodium acetate 5g, ammonium citrate 2g, MgSO
40.2g, MnSO
40.05g with adding 1mL Tween80 behind the 800mL dissolved in distilled water, transferring pH is 6.2～6.6, is settled to 1000mL afterwards, at 121 ℃, 1.034 * 10
5High pressure steam sterilization 20min under the Pa condition;
The composition of LB culture medium: tryptone 10g, yeast 5g, NaCl10g is 7.0 with transferring pH behind the 800mL dissolved in distilled water, is settled to 1000mL afterwards, at 121 ℃, 1.034 * 10
5High pressure steam sterilization 20min under the Pa condition.
3, AFB
1The screening of degradation bacteria strains
With AFB
1Carry out AFB as carbon source and the energy
1The screening of degradation bacteria strains.Qualification result through 26S rDNA shows, screens degraded AFB
1Fungi be aspergillus oryzae, the accession designation number at Chinese microorganism strain preservation center is CGMCC5817.
4, degraded AFB
1The extraction of crude enzyme liquid that aspergillus oryzae produces
Picking degraded AFB
1Aspergillus oryzae strain is coated PDA solid plate with it, results (about 96h) when placing 30 ℃ of constant temperature culture to spore to produce in a large number.In plate, add an amount of sterile saline (Tween80 that contains volume fraction 0.05%), with spreading rod the spore on the plate is scraped, to remove the residual body of mycelia, spore concentration is adjusted into 1 * 10 with four layers of filtered through gauze
8CFU/mL is inoculated in the solid fermentation culture medium by every bottle of 5mL, mixes to be placed in 30 ℃ of constant incubators to gather in the crops behind the cultivation 5d.Solid fermentation culture and physiological saline are mixed soaking at room temperature 1h (be interrupted in the immersion process and stir) according to 1: 2 ratio of solid-to-liquid ratio.Earlier with 8 layers of filtered through gauze fermentation culture medium, afterwards with centrifugal 5min under filtrate 10000 * g condition, filter with qualitative filter paper again after immersion is finished, be stored in 4 ℃ of refrigerators after the membrane filtration degerming with 0.20 μ m at last, be aspergillus oryzae and produce degraded AFB
1Crude enzyme liquid.
5, crude enzyme liquid is to AFB
1Degradation
Choose crude enzyme liquid 6mL (crude enzyme liquid of getting the 6mL high-temperature inactivation is in addition organized in contrast), add AFB
1Make its concentration be about 100 μ g/L, mix and be placed on 30 ℃ of constant temperature culture, sampling and measuring AFB wherein behind the 48h
1Content.Crude enzyme liquid is to AFB after measured
1Degradation rate be 77.05%.Through heat denaturation test proof AFB
1The crude enzyme liquid of digestive enzyme is protein.
6, probio cooperates AFB with crude enzyme liquid
1Degradation
(1) experimental design
(in the ratio combination of above recommending, the content of every kind of bacterium is 1 * 10 with the probio combination
9CFU/mL) with degraded AFB
1Crude enzyme liquid is allocated by the portfolio ratio of table 1, makes the final volume of reaction system be 60mL.Add AFB
1Make its ultimate density in system be about 50 μ g/L, be placed on shaken cultivation in 37 ℃ of constant temperature gas bath oscillators.The AFB that adds Isodose with the PBS buffer solution of equal volume
1As blank.
The combination of table 1 probio and degraded AFB
1The crude enzyme liquid compatibility is to the experimental design of the degraded of AFB1
(2) probio combination and crude enzyme liquid compatibility are to AFB
1The degraded supernatant in AFB
1Measure
Nutrient solution is mixed, draw 1mL with pipettor, centrifugal 5min under 13,000 rev/mins of conditions gets centrifugal back supernatant and is used for the AFB1 assay.
(3) probio combination and crude enzyme liquid compatibility are to AFB
1Degradation
As can be seen from Figure 1, the 5th processed group is to AFB
1Degradation rate the highest, degradation rate reaches 78.07%, and probio combination and AFB are described
1The digestive enzyme crude enzyme liquid with 2: 3 ratio compatibility to AFB
1Degradation effect best.Independent probio combination or AFB1 digestive enzyme are to AFB
1Degradation rate be respectively 69.48% and 70.46%.Prove absolutely probio combination and AFB
1Digestive enzyme has good compatibility effect.
AFB of the present invention
1The application of degradation agent in broiler chicken is produced.After concrete the application, obtain drawing a conclusion:
(1) probio combination and AFB
1Digestive enzyme is pressed 2: 3 portfolio ratio to AFB
1Degradation effect best, to AFB
1Degradation rate reach 78.07%.
(2) at AFB
1Content is to add 0.15% probio and AFB in the broiler fodder of 200 μ g/kg
1Digestive enzyme can significantly be alleviated AFB
1Harmful effect to meat chicken production performance.
(3) the genetic chip testing result shows, adds probio and AFB
1Digestive enzyme has significantly been alleviated the problem because of gene expression amount generation adverse changes such as the oxidoreducing enzyme system that influences the production performance performance that interpolation AFB1 causes, Apoptosis related enzyme systems, cell growth related enzyme systems, immune system process control enzyme system and metabolic process control enzyme systems.
The above; only be the preferable specific embodiment of the present invention; protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the simple change of the technical scheme that can obtain apparently or equivalence are replaced and are all fallen within the scope of protection of the present invention.
Claims (2)
1. AFB
1Degradation agent is characterized in that, comprises composition a and composition b, and its portfolio ratio is 2: 3, wherein:
The preparation method of described composition a is: butter bacillus CGMCC1.62, bacillus subtilis CGMCC1.504, unusual Hansenula yeast CGMCC2.881, cultivate 48h separately after, mix the back with 2: 1: 1 ratios and load the body hypothermia drying;
The preparation method of described composition b is: with AFB
1Carry out AFB as carbon source and the energy
1The screening of degradation bacteria strains, the qualification result demonstration through 26SrDNA screens degraded AFB
1Fungi be aspergillus oryzae, the accession designation number at Chinese microorganism strain preservation center is CGMCC5817, to the degraded AFB
1Fungi carry out solid fermentation and cultivate, extract AFB
1The digestive enzyme crude enzyme liquid, low temperature drying behind the adding carrier;
Composition a and composition b, in making the AFB1 degradation agent after the ratio mixing in 2: 3, wherein the content of butter bacillus, bacillus subtilis and unusual three kinds of bacterium of Hansenula yeast is respectively 2 * 10
9Individual/g, 1 * 10
9Individual/g and 1 * 10
9Individual/g, AFB
1The digestive enzyme vigor is 135U/g, AFB
1The digestive enzyme vigor is defined as: per minute degraded 1ng AFB under 37 ℃ condition
1It is enzyme unit alive.
2. the described AFB of claim 1
1The application of degradation agent in broiler chicken is produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310067190.4A CN103190538B (en) | 2013-02-23 | 2013-02-23 | Aflatoxin B1 degradation agent and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310067190.4A CN103190538B (en) | 2013-02-23 | 2013-02-23 | Aflatoxin B1 degradation agent and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103190538A true CN103190538A (en) | 2013-07-10 |
| CN103190538B CN103190538B (en) | 2015-01-28 |
Family
ID=48713504
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310067190.4A Active CN103190538B (en) | 2013-02-23 | 2013-02-23 | Aflatoxin B1 degradation agent and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103190538B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820353A (en) * | 2013-11-08 | 2014-05-28 | 山东农业大学 | Bacillus subtilis for degrading AFB1 (Aflatoxius B1) |
| CN104738363A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Uses of rhodococcus erythropolis in degradation of aflatoxin B1 in feed or raw materials of the feed |
| CN105219677A (en) * | 2015-10-26 | 2016-01-06 | 山东华牧天元农牧股份有限公司 | A kind of complex microorganism preparations of aflatoxin degradation and application thereof |
| CN106261474A (en) * | 2016-08-08 | 2017-01-04 | 广东海洋大学 | A kind of utilize the method for mycotoxin in lactic acid bacteria degraded food |
| CN106860878A (en) * | 2017-01-13 | 2017-06-20 | 华中农业大学 | MT genes are used as preventing and treating AFB1Cause the application of duck hepar damnification target gene |
| CN108865901A (en) * | 2018-07-17 | 2018-11-23 | 山东省科学院生物研究所 | One Aspergillus oryzae bacterial strain and its application in aflatoxin degradation |
| CN108893434A (en) * | 2018-07-21 | 2018-11-27 | 泸州正泰生物工程有限公司 | A kind of microbiological garbage treatment agent and the preparation method and application thereof of degrading aspergillus flavus element B1 |
| CN111281970A (en) * | 2018-12-10 | 2020-06-16 | 河南普爱饲料股份有限公司 | Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating mycotoxin-induced epithelial cell injury |
| CN113521115A (en) * | 2021-07-15 | 2021-10-22 | 河南普爱饲料股份有限公司 | Aflatoxin detoxification composition and preparation method and application thereof |
| CN115281316A (en) * | 2022-06-24 | 2022-11-04 | 黄淮学院 | Composite fermentation microbial inoculum for degrading mycotoxin in meat product and preparation method of high-digestibility fermented dried chicken |
| CN116064332A (en) * | 2023-02-01 | 2023-05-05 | 山东省花生研究所 | Bacterial strain for degrading aflatoxin B1 and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107937357A (en) * | 2016-10-12 | 2018-04-20 | 河南工业大学 | The preparation method of complex microorganism preparations efficient degradation aflatoxin B1 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1364421A (en) * | 2001-01-12 | 2002-08-21 | 暨南大学 | Detoxicaction method for aflatoxin in fodder |
| CN101238866A (en) * | 2008-03-05 | 2008-08-13 | 仲恺农业技术学院 | Degradation method of aflatoxin |
| CN101525633A (en) * | 2009-04-13 | 2009-09-09 | 天津科技大学 | Method for preparing plant pathomycete zymotic fluid produced by lactobacillus |
| CN101720848A (en) * | 2009-12-09 | 2010-06-09 | 中国农业科学院饲料研究所 | Complex enzyme for biodegrading mycotoxin in feed |
| CN102286378A (en) * | 2011-02-25 | 2011-12-21 | 河南普爱饲料股份有限公司 | Composite probiotics for inhabiting aspergillus flavus growth and degrading aflatoxin and application thereof |
| CN102925361A (en) * | 2012-08-10 | 2013-02-13 | 河南普爱饲料股份有限公司 | AFB1 degrading bacterium and degrading enzyme |
-
2013
- 2013-02-23 CN CN201310067190.4A patent/CN103190538B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1364421A (en) * | 2001-01-12 | 2002-08-21 | 暨南大学 | Detoxicaction method for aflatoxin in fodder |
| CN101238866A (en) * | 2008-03-05 | 2008-08-13 | 仲恺农业技术学院 | Degradation method of aflatoxin |
| CN101525633A (en) * | 2009-04-13 | 2009-09-09 | 天津科技大学 | Method for preparing plant pathomycete zymotic fluid produced by lactobacillus |
| CN101720848A (en) * | 2009-12-09 | 2010-06-09 | 中国农业科学院饲料研究所 | Complex enzyme for biodegrading mycotoxin in feed |
| CN102286378A (en) * | 2011-02-25 | 2011-12-21 | 河南普爱饲料股份有限公司 | Composite probiotics for inhabiting aspergillus flavus growth and degrading aflatoxin and application thereof |
| CN102925361A (en) * | 2012-08-10 | 2013-02-13 | 河南普爱饲料股份有限公司 | AFB1 degrading bacterium and degrading enzyme |
Non-Patent Citations (1)
| Title |
|---|
| RUIYU ZUO 等: "Inhibiting Aspergillus flavus growth and degrading aflatoxin B1 by combined beneficial microbes", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103820353B (en) * | 2013-11-08 | 2015-10-14 | 山东农业大学 | The subtilis of one strain degraded AFB1 |
| CN103820353A (en) * | 2013-11-08 | 2014-05-28 | 山东农业大学 | Bacillus subtilis for degrading AFB1 (Aflatoxius B1) |
| CN104738363B (en) * | 2013-12-27 | 2019-10-15 | 中粮营养健康研究院有限公司 | Purposes of the red city Rhodococcus sp in the aflatoxin B1 in degradation feed or its raw material |
| CN104738363A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Uses of rhodococcus erythropolis in degradation of aflatoxin B1 in feed or raw materials of the feed |
| CN105219677A (en) * | 2015-10-26 | 2016-01-06 | 山东华牧天元农牧股份有限公司 | A kind of complex microorganism preparations of aflatoxin degradation and application thereof |
| CN106261474A (en) * | 2016-08-08 | 2017-01-04 | 广东海洋大学 | A kind of utilize the method for mycotoxin in lactic acid bacteria degraded food |
| CN106860878A (en) * | 2017-01-13 | 2017-06-20 | 华中农业大学 | MT genes are used as preventing and treating AFB1Cause the application of duck hepar damnification target gene |
| CN108865901A (en) * | 2018-07-17 | 2018-11-23 | 山东省科学院生物研究所 | One Aspergillus oryzae bacterial strain and its application in aflatoxin degradation |
| CN108865901B (en) * | 2018-07-17 | 2020-06-09 | 山东省科学院生物研究所 | Aspergillus oryzae strain and application thereof in aflatoxin degradation |
| CN108893434A (en) * | 2018-07-21 | 2018-11-27 | 泸州正泰生物工程有限公司 | A kind of microbiological garbage treatment agent and the preparation method and application thereof of degrading aspergillus flavus element B1 |
| CN111281970A (en) * | 2018-12-10 | 2020-06-16 | 河南普爱饲料股份有限公司 | Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating mycotoxin-induced epithelial cell injury |
| CN113521115A (en) * | 2021-07-15 | 2021-10-22 | 河南普爱饲料股份有限公司 | Aflatoxin detoxification composition and preparation method and application thereof |
| CN113521115B (en) * | 2021-07-15 | 2023-12-29 | 河南普爱饲料股份有限公司 | Aflatoxin detoxification composition and preparation method and application thereof |
| CN115281316A (en) * | 2022-06-24 | 2022-11-04 | 黄淮学院 | Composite fermentation microbial inoculum for degrading mycotoxin in meat product and preparation method of high-digestibility fermented dried chicken |
| CN116064332A (en) * | 2023-02-01 | 2023-05-05 | 山东省花生研究所 | Bacterial strain for degrading aflatoxin B1 and application thereof |
| CN116064332B (en) * | 2023-02-01 | 2023-08-15 | 山东省花生研究所 | Bacterial strain for degrading aflatoxin B1 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103190538B (en) | 2015-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103190538B (en) | Aflatoxin B1 degradation agent and application thereof | |
| CN101333499B (en) | Complex active bacterial biological water purifying a gent and method for preparing same | |
| CN103798503B (en) | The ruminant Tiny ecosystem functional feed of composite zymocyte liquid and preparation method | |
| CN108949739A (en) | A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater | |
| CN105132322A (en) | Lactobacillus plantarum and application thereof | |
| CN107974421A (en) | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum | |
| CN113736716B (en) | Lactobacillus paracasei, compound biological leavening agent for yellow storage and yellow storage method | |
| CN109082393A (en) | One kind can be used for Treating Municipal Sewage microbial bacterial agent and preparation method thereof | |
| CN114921385B (en) | Bacillus subtilis and application thereof in feed addition and antibiotic-free cultivation | |
| CN105132307B (en) | It is a kind of applied to the composite bacillus microbial water-purifying agent of ornamental fish and its application | |
| CN107201322A (en) | Bacillus subtilis and its application for degrading aflatoxin B 1 | |
| CN109423465A (en) | A kind of control aflatoxin B1Composite biological agent and its application | |
| CN104855684A (en) | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof | |
| CN106854627A (en) | A kind of composite bacteria agent for promoting the flat angle stratiomyiid larval growth of speck and application | |
| CN102936568A (en) | Solid fermentation inoculum of chicken-origin bacillus subtilis and pediococcus pentosaceus and preparation method and application of solid fermentation inoculum | |
| CN109043122A (en) | For the liquid bacterium solution of fermentation cassava dreg fodder and its preparation method of Starch Tapioca residue fermented feed | |
| CN105132321A (en) | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method | |
| CN102286378B (en) | Composite probiotics for inhabiting aspergillus flavus growth and degrading aflatoxin and application thereof | |
| CN111909878A (en) | Lactobacillus casei with function of removing patulin | |
| CN107779422B (en) | Non-decarboxylation lecanium biocontrol strain for efficiently inhibiting aspergillus flavus from synthesizing aflatoxin | |
| CN112760274B (en) | Organic solid waste high-temperature aerobic composting strain and application thereof | |
| CN113980853A (en) | Lactococcus garvieae WBT0008 capable of producing lactic acid at high yield and application thereof | |
| CN110452859B (en) | Bacillus amyloliquefaciens B4216 and application thereof | |
| CN110452849B (en) | Probiotic lactobacillus plantarum | |
| CN105586286A (en) | Method for producing heavy metal absorbent through microbiological treatment on lignite and related compound microbial agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: LUOYANG OKBK BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: ZHENGZHOU OUKEBAI BIOLOGICAL TECHNOLOGY CO., LTD. Effective date: 20131118 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yin Qingqiang Inventor after: Zuo Ruiyu Inventor after: Wang Ping Inventor after: Chang Juan Inventor after: Zheng Qiuhong Inventor after: Liu Zhiyong Inventor after: Fan Shouwu Inventor before: Yin Qingqiang Inventor before: Zuo Ruiyu Inventor before: Wang Ping Inventor before: Chang Juan Inventor before: Zheng Qiuhong Inventor before: Liu Zhiyong Inventor before: Fan Shouwu |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 450001 ZHENGZHOU, HENAN PROVINCE TO: 471000 LUOYANG, HENAN PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20131118 Address after: 471000 north side of Hongqi Road, Chengguan Town, Yiyang County, Luoyang, Henan Applicant after: ZHENGZHOU OUKEBAI BIOLOGICAL TECHNOLOGY CO., LTD. Address before: 5, building 4, building 4, 450001, National Science Park, National University Science Park, Zhengzhou, Henan Applicant before: Zhengzhou Oukebai Biological Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: ZHENGZHOU OUKEBAI BIOLOGICAL TECHNOLOGY CO., LTD. Document name: Notification of Passing Examination on Formalities |