CN104855684A - An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof - Google Patents
An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof Download PDFInfo
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Abstract
The present invention provides an antimicrobial traditional Chinese medicine microbial fermentation preparation which uses traditional Chinese medicines as raw materials and is fermented by lactobacillus acidophilus and pediococcus pentosaceus collaboratively; the traditional Chinese medicines consist of portulaca oleracea, forsythia suspensa or phellodendron chinense bark. The present invention also provides a preparation method and application of the above antimicrobial traditional Chinese medicine microbial fermentation preparation. In summary, the traditional Chinese medicine microbial fermentation preparation can be more effective in treating piglets' diarrhea than antibiotics, has low side effects, can replace the use of antibiotics. Meanwhile, the traditional Chinese medicine microbial fermentation preparation has more significant inhibition effects for clinical pathogenic bacteria Escherichia coli and Staphylococcus aureus than the separate use of bacteria and traditional Chinese medicines, and has excellent clinical application prospect.
Description
Technical field
The present invention relates to a kind of antibacterial Chinese medicine microbial fermentation preparation and its production and use.
Background technology
At present, abuse of antibiotics causes flora imbalance and has become the common phenomenon of veterinary clinic, and for solving abuse of antibiotics problem, Chinese scholars investigated many feasible measures, and wherein probiotics is the most promising product being considered to substitute antibiotics.Chinese herbal medicine has with a long history, the advantage such as aboundresources, complete function, toxic and side effect are little, noresidue in China.Microorganism has very abundant enzyme system, has powerful decomposition, the ability of transformation substance, uses fermentable Chinese medicine, may carry out biology conversion to chemical composition of Chinese materia medica, produces new compound or causes the change of some component contents in Chinese medicine.In addition, in the process of fermentable Chinese medicine, some metabolic pathway of some compositions possibility inducing microbial in Chinese medicine changes, thus produces new compound.Screening for reactive compound is all provided rich in natural resources by these.Fermentable Chinese medicine should become the fresh content of traditional Chinese medicine research.
Lactobacillus acidophilus (Lactobacillus acidopphilus) is a kind of common probio, being colonizated in one of the bacterium of being everlasting in animal gastrointestinal tract system, is can produce the material such as lactic acid, antibacterial peptide in its growth course to the important function of animal body.It is added in feed, in raising growth of animals or poultry speed, strengthen immunity of organisms, prevention and therapy epidemic diarrhea, improve young animal survival rate, improve efficiency of feed utilization etc. in play significant effect.Pediococcus pentosaceus (Pediococcus pentosaceus) belongs to gram-positive cocci, Streptococcaceae, Pediococcus, and glucose fermentation produces lactic acid, is distributed in brewer's wort and fermenting plant material, as in sour salted vegetables, pickles, ensilage etc.Pediococcus pentosaceus is only widely used in the field such as greenfeed, fermentation meat product so far, and it is also rarely had report as the research of animal feed additive.Pediococcus pentosaceus is as a kind of lactic acid bacteria, the resistance of reverse of itself, Pediococcus pentosaceus adds in ferment sausage by Zhang Fengkuan etc., find that total viable count and lactic acid bacteria number increase significantly in ferment sausage maturation, effectively can suppress the growth of harmful bacteria, and significantly reduce the nitrate residue in sausage.
Have no lactobacillus acidophilus and Pediococcus pentosaceus co-fermentation Chinese medicine, prepare the report of Chinese medicine microbe leaven.
Summary of the invention
The object of the present invention is to provide a kind of antibacterial Chinese medicine microbial fermentation preparation and its production and use.
The invention provides a kind of antibacterial Chinese medicine microbial fermentation preparation, it take Chinese medicine as raw material, is prepared from through lactobacterium acidophilus and Pediococcus pentosaceus co-fermentation; Described Chinese medicine is purslane, the capsule of weeping forsythia or golden cypress.
Wherein, described Chinese medicine is purslane, the capsule of weeping forsythia or golden cypress.
Wherein, the number ratio of described lactobacterium acidophilus and Pediococcus pentosaceus is 1:1.
Wherein, described every 1g Chinese medicine, adopts 0.6 × 10
8the bacterium fermentation of CFU.
Wherein, described preparation is it is prepare by the following method:
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus is got according to proportioning;
(2) Feedstock treating: Chinese medicine is pulverized, adds water or the MRS culture medium of Chinese medicine 6 times of weight, mixing, sterilizing, then add MRS culture medium, addition is 20 times of v/w of Chinese medicine, obtains material liquid;
(3) bacteria suspension is prepared: respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, make clump count and be 10
8the bacteria suspension of CFU/ml;
(4) ferment: add in the material liquid of step (2) by two kinds of bacteria suspensions of step (3), the addition of two kinds of bacteria suspensions is 0.3 times of v/w of Chinese medicine, cultivates 10h, to obtain final product under 37 DEG C of conditions simultaneously.
Wherein, described formulation is the preparation through gastrointestinal administration.Preferably, described preparation is oral liquid, tablet, pill, granule or capsule.
Present invention also offers the preparation method of aforementioned Chinese medicine microbial fermentation preparation, it comprises following operating procedure:
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus is got according to proportioning;
(2) Feedstock treating: Chinese medicine is pulverized, adds water or the MRS culture medium of Chinese medicine 6 times of weight, mixing, sterilizing, then add MRS culture medium, addition is 20 times of v/w of Chinese medicine, obtains material liquid;
(3) bacteria suspension is prepared: respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, make clump count and be 10
8the bacteria suspension of CFU/ml;
(4) ferment: add in the material liquid of step (2) by two kinds of bacteria suspensions of step (3), the addition of two kinds of bacteria suspensions is 0.3 times of v/w of Chinese medicine, cultivates 10h, to obtain final product under 37 DEG C of conditions simultaneously.
Present invention also offers the purposes of aforementioned Chinese medicine microbial fermentation preparation in the antibacterial medicine of preparation or feed addictive.
Wherein, described antibacterial medicine or feed addictive are medicine or the feed addictives of Chinese People's Anti-Japanese Military and Political College enterobacteria and/or staphylococcus aureus.
Present invention also offers the purposes of aforementioned Chinese medicine microbial fermentation preparation in the herbal medicine of preparation treatment grice diarrhoea.
Chinese medicine microbe leaven of the present invention effectively can treat grice diarrhoea, successful is better than antibiotic, and side effect is little, can use by substitute antibiotics, meanwhile, Chinese medicine microbe leaven of the present invention, has obvious inhibitory action to clinical cure the disease bacterium Escherichia coli and staphylococcus aureus, effect is all better than bacterium and Chinese medicine is used alone, and potential applicability in clinical practice is excellent.
Detailed description of the invention below in conjunction with embodiment is described in further detail foregoing of the present invention again.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example.Without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Detailed description of the invention
The preparation of embodiment 1 Chinese medicine microbial fermentation preparation of the present invention
1.1 experiment material
1.1.1 bacterial strain: Pediococcus pentosaceus (deposit number
21862), lactobacillus acidophilus (deposit number
20710), E.coli K88, K99,987P reference culture, staphylococcus aureus is bought by Chinese industrial Microbiological Culture Collection administrative center (CICC).
1.1.2 culture medium.1. MRS fluid nutrient medium: for cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water adds to 1000ml, adjusts pH to 6.0.2. MRS solid medium: for cultivating lactobacillus acidophilus.The agar of 1.5%-2.0% is added in the ship s fluid nutrient medium made.3. beef-protein medium: bring enterobacteria and staphylococcus aureus up.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water adds to 1000ml, adjusts pH to 7.2-7.6.
Chinese medicine: purslane
1.2 experimental technique
1.2.1 seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 1 × 10
8the seed liquor of CFU/mL.
Herb fermenting: after purslane section is pulverized, get 5g in the triangular flask of 50mL, add 30mL distilled water, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
The preparation of embodiment 2 Chinese medicine microbial fermentation preparation of the present invention
1.1 experiment material
1.1.1 bacterial strain: Pediococcus pentosaceus (deposit number
21862), lactobacillus acidophilus (deposit number
20710), E.coli K88, K99,987P reference culture, staphylococcus aureus is bought by Chinese industrial Microbiological Culture Collection administrative center (CICC).
1.1.2 culture medium.1. MRS fluid nutrient medium: for cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water adds to 1000ml, adjusts pH to 6.0.2. MRS solid medium: for cultivating lactobacillus acidophilus.The agar of 1.5%-2.0% is added in the ship s fluid nutrient medium made.3. beef-protein medium: bring enterobacteria and staphylococcus aureus up.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water adds to 1000ml, adjusts pH to 7.2-7.6.
Chinese medicine: the capsule of weeping forsythia
1.2 experimental technique
1.2.1 seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 1 × 10
8the seed liquor of CFU/mL.
Herb fermenting: after capsule of weeping forsythia section is pulverized, get 5g in the triangular flask of 50mL, add 30mL distilled water, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
The preparation of embodiment 3 Chinese medicine microbial fermentation preparation of the present invention
1.1 experiment material
1.1.1 bacterial strain: Pediococcus pentosaceus (deposit number
21862), lactobacillus acidophilus (deposit number
20710), E.coli K88, K99,987P reference culture, staphylococcus aureus is bought by Chinese industrial Microbiological Culture Collection administrative center (CICC).
1.1.2 culture medium.1. MRS fluid nutrient medium: for cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water adds to 1000ml, adjusts pH to 6.0.2. MRS solid medium: for cultivating lactobacillus acidophilus.The agar of 1.5%-2.0% is added in the ship s fluid nutrient medium made.3. beef-protein medium: bring enterobacteria and staphylococcus aureus up.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water adds to 1000ml, adjusts pH to 7.2-7.6.
Chinese medicine: golden cypress
1.2 experimental technique
1.2.1 seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 1 × 10
8the seed liquor of CFU/mL.
Herb fermenting: after golden cypress section is pulverized, get 5g in the triangular flask of 50mL, add 30mL distilled water, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
Beneficial effect of the present invention is illustrated below by way of test example.
The Antibacterial Activity of experimental example 1 Chinese medicine microbial fermentation preparation of the present invention
1.1 experiment material
1.1.1 bacterial strain: Pediococcus pentosaceus (deposit number
21862), lactobacillus acidophilus (deposit number
20710), E.coli K88, K99,987P reference culture, staphylococcus aureus is bought by Chinese industrial Microbiological Culture Collection administrative center (CICC).
1.1.2 culture medium.1. MRS fluid nutrient medium: for cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, yeast extract 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water adds to 1000ml, adjusts pH to 6.0.2. MRS solid medium: for cultivating lactobacillus acidophilus.The agar of 1.5%-2.0% is added in the ship s fluid nutrient medium made.3. beef-protein medium: bring enterobacteria and staphylococcus aureus up.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water adds to 1000ml, adjusts pH to 7.2-7.6.
Chinese medicine: purslane, the capsule of weeping forsythia, golden cypress
1.2 experimental technique
1.2.1 the preparation of experiment material
Grouping: be divided into four groups, I Chinese medicine+water+bacterium; II Chinese medicine+culture medium+bacterium; III Chinese medicine+culture medium; IV culture medium+bacterium.
Group I: Chinese medicine microbial fermentation preparation 1 of the present invention
Seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 1 × 10
8the seed liquor of CFU/mL.
Herb fermenting: by purslane, after the capsule of weeping forsythia or golden cypress section are pulverized, get 5g in the triangular flask of 50mL, add 30mL distilled water, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
Experimental group II: Chinese medicine microbial fermentation preparation 2 of the present invention
Seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 10
8the seed liquor of CFU/mL.
Herb fermenting: by purslane, after the capsule of weeping forsythia or golden cypress section are pulverized, get 5g in the triangular flask of 50mL, add 30mLMRS culture medium, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
Experimental group III: contrast 1
By purslane, the capsule of weeping forsythia or golden cypress section are got 5g in the triangular flask of 50mL, are added 30mLMRS culture medium, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL after pulverizing.
Experimental group IV: contrast 2
Seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 10
8the seed liquor of CFU/mL.Normal fermentation: get in the triangular flask (250mL) of the MRS culture medium of 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
Whether write according to your material, it is accurate to examine.
1.2.2 sample treatment: after 10d is cultivated in the fermentate of different Chinese medicine and each group contrast, Filter paper filtering, filtrate concentrates, and is settled to 5mL.Directly get 100 μ L during use and carry out Activity determination.
1.2.3 bacteriostatic experiment (with Odontothrips loti): adopt double-deck agar plate to be that indicator bacteria is dull and stereotyped, bottom adds the nutrient agar of 15mL 2%.After bottom cooling, put into the Oxford cup that several external diameter is 6mm, getting 1mL pathogenic bacteria bacteria suspension, to join the agar concentration that 15mL is cooled to about 45 DEG C be in the culture medium of 1%, mixes rapidly, be poured on nutrient agar, with aseptic nipper, Oxford cup is taken out after cooling, namely some holes are formed, respectively to interior injection 200 μ L fermented supernatant fluid, 37 DEG C of constant temperature culture 24h, observe inhibition zone size around hole, and measure transparent circle diameter with slide measure.The bacteriostatic experiment result that the sample observing each group of process does, the size of the measurement inhibition zone having inhibition zone to produce, what do not have inhibition zone to generate thinks that this process does not have bacteriostasis under this dosage, and antibacterial circle diameter is less than thinking of 7mm and only has Weak activity.
1.3 results and analysis
Chinese medicine microecological preparation of the present invention is to the fungistatic effect of pathogenic bacteria
Note :-there is no inhibitory action.
As can be seen from the above table, relative to blank, Chinese medicine microbial fermentation preparation 1 and 2 (group I and group II) of the present invention can effectively suppress Escherichia coli and staphylococcus aureus; In addition, compared with the pure Portulaca oleracea L of same dose, the capsule of weeping forsythia, golden cypress (organizing III) and pure probio normal fermentation (organizing IV), the inhibition zone of Chinese medicine microbial fermentation preparation of the present invention is obviously larger, and fungistatic effect is obviously better.
Experimental result illustrates, the inhibit activities of Chinese medicine microbial fermentation preparation of the present invention to clinical bacteria Escherichia coli and staphylococcus aureus is excellent, can be used for treating this two kinds of bacterial infectious diseases.
The effect of experimental example 2 Chinese medicine microbial fermentation preparation treatment of the present invention grice diarrhoea
The preparation of 1 Chinese medicine microbial fermentation preparation of the present invention
Seed liquor: lactobacterium acidophilus and Pediococcus pentosaceus individually expanded at the suspension that MRS slant medium is produced and be incubated in MRS meat soup, 37 DEG C of constant temperature culture 48h, making clump count is 10
8the seed liquor of CFU/mL.
Herb fermenting: by purslane, after the capsule of weeping forsythia or golden cypress section are pulverized, get 5g in the triangular flask of 50mL, add 30mL distilled water, 121 DEG C of sterilizing 20min, join in the triangular flask (250mL) of the MRS culture medium containing 100mL, and then add lactobacillus acidophilus and each 1.5mL of Pediococcus pentosaceus seed liquor, 37 DEG C of constant temperature culture 10d.
With aureomycin in contrast.
2 test methods
The pig farm yellowish-white dysentery occurring in Yaan is tested, and this piglet in delivery room in December shows as violent diarrhoea, excreta stench, and yellowly pasty state or water sample are diagnosed as the coliform diarrhea of piglet through clinical diagnosis and test in laboratory.By occur diarrhoea piglet by 30 often group divide into groups, always be divided into 4 groups, the diarrhoea situation of observation experiment group and control group respectively, and it is efficient to record after 7 days, random selecting piglet 3 is not organized after treatment terminates, be separated jejunal segment and get its jejunal mucous membrane, with the content of its SIgA, IL-2 of Elisa kit measurement.
3 results and analysis
Experimental result is as shown in the table:
As can be seen from the above table, after adopting the present invention's three kinds of Chinese medicine microbial fermentation preparation treatments, grice diarrhoea all obviously declines, the content of Jejunum of Piglets mucous membrane proinflammatory cytokine IL-2 significantly reduces, the amount of the IgA antibody of secreting type significantly increases, illustrate that the present invention's three kinds of Chinese medicine microbial fermentation preparations effectively can treat grice diarrhoea, and successful is better than positive drug aureomycin.
Meanwhile, the outcome of Chinese medicine microbial fermentation preparation of the present invention is obviously better than aureomycin, and the piglet later stage after aureomycin treatment becomes cad pig, the piglet after Chinese medicine microorganism formulation of the present invention treatment then with normal piglets no significant difference.
To sum up, Chinese medicine microbe leaven of the present invention effectively can treat grice diarrhoea, successful is better than antibiotic, and side effect is little, can use by substitute antibiotics, simultaneously, Chinese medicine microbe leaven of the present invention, have obvious inhibitory action to clinical cure the disease bacterium Escherichia coli and staphylococcus aureus, effect is all better than bacterium and Chinese medicine is used alone, and potential applicability in clinical practice is excellent.
Claims (10)
1. an antibacterial Chinese medicine microbial fermentation preparation, is characterized in that: it take Chinese medicine as raw material, is prepared from through lactobacterium acidophilus and Pediococcus pentosaceus co-fermentation; Described Chinese medicine is purslane, the capsule of weeping forsythia or golden cypress.
2. preparation according to claim 1, is characterized in that: the number ratio of described lactobacterium acidophilus and Pediococcus pentosaceus is 1:1.
3. preparation according to claim 1 and 2, is characterized in that: described every 1g Chinese medicine, adopts 0.6 × 10
8the bacterium fermentation of CFU.
4. the preparation according to claims 1 to 3 any one, is characterized in that: it prepares by the following method:
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus is got according to proportioning;
(2) Feedstock treating: Chinese medicine is pulverized, adds water or the MRS culture medium of Chinese medicine 6 times of weight, mixing, sterilizing, then add MRS culture medium, addition is 20 times of v/w of Chinese medicine, obtains material liquid;
(3) bacteria suspension is prepared: respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, make clump count and be 10
8the bacteria suspension of CFU/ml;
(4) ferment: add in the material liquid of step (2) by two kinds of bacteria suspensions of step (3), the addition of two kinds of bacteria suspensions is 0.3 times of v/w of Chinese medicine, cultivates 10h, to obtain final product under 37 DEG C of conditions simultaneously.
5. the preparation according to Claims 1 to 4 any one, is characterized in that: described formulation is the preparation through gastrointestinal administration.
6. preparation according to claim 5, is characterized in that: described preparation is oral liquid, tablet, pill, granule or capsule.
7. the preparation method of Chinese medicine microbial fermentation preparation described in claim 1 ~ 6 any one, is characterized in that: it comprises following operating procedure:
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus is got according to proportioning;
(2) Feedstock treating: Chinese medicine is pulverized, adds water or the MRS culture medium of Chinese medicine 6 times of weight, mixing, sterilizing, then add MRS culture medium, addition is 20 times of v/w of Chinese medicine, obtains material liquid;
(3) bacteria suspension is prepared: respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, make clump count and be 10
8the bacteria suspension of CFU/ml;
(4) ferment: add in the material liquid of step (2) by two kinds of bacteria suspensions of step (3), the addition of two kinds of bacteria suspensions is 0.3 times of v/w of Chinese medicine, cultivates 10h, to obtain final product under 37 DEG C of conditions simultaneously.
8. the purposes of Chinese medicine microbial fermentation preparation described in claim 1 ~ 6 any one in the antibacterial medicine of preparation or feed addictive.
9. purposes according to claim 8, is characterized in that: described antibacterial medicine or feed addictive are medicine or the feed addictive of Chinese People's Anti-Japanese Military and Political College enterobacteria and/or staphylococcus aureus.
10. the purposes of Chinese medicine microbial fermentation preparation described in claim 1 ~ 6 any one in the herbal medicine of preparation treatment grice diarrhoea.
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| CN109123141A (en) * | 2018-08-28 | 2019-01-04 | 河南邑鸿善成生物技术有限公司 | A kind of application of preparation method of fermented tcm dreg fodder and products thereof, product |
| CN109350648A (en) * | 2018-10-10 | 2019-02-19 | 广州睿森生物科技有限公司 | A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof |
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| CN110201099A (en) * | 2019-06-14 | 2019-09-06 | 四川辅正药业股份有限公司 | The method of Pediococcus pentosaceus pure-blood ferment Medicated Leaven |
| CN114041536A (en) * | 2021-11-24 | 2022-02-15 | 石河子大学 | Composite Chinese herbal medicine microecological preparation and preparation method thereof |
| CN117562941A (en) * | 2024-01-15 | 2024-02-20 | 晏龙国科(山东)微生物科技有限公司 | Bidirectional fermentation process of traditional Chinese medicine lactic acid bacteria |
| CN117562941B (en) * | 2024-01-15 | 2024-03-29 | 晏龙国科(山东)微生物科技有限公司 | Veterinary traditional Chinese medicine lactobacillus bidirectional fermentation process |
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