CN104855684B - A kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application - Google Patents
A kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application Download PDFInfo
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Abstract
The present invention provides a kind of antibacterial Chinese medicine microbial fermentation preparation, it is using Chinese medicine as raw material, is prepared through lactobacterium acidophilus and Pediococcus pentosaceus co-fermentation；The Chinese medicine is purslane, Fructus Forsythiae or Cortex Phellodendri.Present invention also offers the preparation method and purposes of foregoing antibacterial Chinese medicine microbial fermentation preparation.To sum up, Chinese medicine microbe leaven of the present invention can effectively treat grice diarrhoea, positive effect is better than antibiotic, and Small side effects, it can be used with substitute antibiotics, at the same time, Chinese medicine microbe leaven of the present invention, there is obvious inhibitory action to clinical pathogen Escherichia coli and staphylococcus aureus, effect is superior to bacterium and Chinese medicine is used alone, and potential applicability in clinical practice is excellent.
Description
Technical field
The present invention relates to a kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application.
Background technology
At present, abuse of antibiotics triggers flora imbalance to become the common phenomenon of veterinary clinic, to solve abuse of antibiotics
Problem, domestic and foreign scholars investigated many feasible measures, before wherein probiotics is considered as most the having of substitute antibiotics
The product on way.Chinese herbal medicine has the advantages such as with a long history, aboundresources, full-featured, toxic side effect is small, noresidue in China.
Microorganism has very abundant enzyme system, there is powerful decomposition, the ability of transformation substance, may with microbial fermentation Chinese medicine
Bioconversion is carried out to chemical composition of Chinese materia medica, new compound is produced or causes the change of some component contents in Chinese medicine.In addition,
During microbial fermentation Chinese medicine, some metabolic pathways of the possible inducing microbial of some components in Chinese medicine change,
So as to produce new compound.These all will provide abundant resource for the screening of reactive compound.Microbial fermentation Chinese medicine should
As the new content of traditional Chinese medicine research.
Lactobacillus acidophilus (Lactobacillus acidopphilus) is a kind of common probiotics, is colonized in animal stomach
Normal in one of bacterium in intestinal tract, the important function to animal body is that lactic acid, antibacterial peptide can be produced during its growth
Deng material.Add it in feed, improve growth of animals or poultry speed, enhancing immunity of organisms, prevention and treatment epidemic diarrhea,
Improve young animal survival rate, improvement efficiency of feed utilization etc. and play obvious action.Pediococcus pentosaceus
(Pediococcus pentosaceus) belongs to gram-positive cocci, Streptococcaceae, Pediococcus, glucose fermentation generation
Lactic acid, is distributed in brewer's wort and fermenting plant material, in such as sour pickle, pickles, ensilage.Pediococcus pentosaceus is only so far
It is widely used in the fields such as greenfeed, fermentation meat product, is also rarely reported it as the research of animal feed additive.Penta
Sugar-tablet coccus adds Pediococcus pentosaceus in ferment sausage as a kind of lactic acid bacteria, the resistance of reverse of itself, Zhang Fengkuan etc., finds
Total viable count and lactic acid bacteria number significantly increase in ferment sausage maturation, can effectively suppress the growth of harmful bacteria, and show
Write the nitrate residue reduced in sausage.
Have no the report for by lactobacillus acidophilus and Pediococcus pentosaceus co-fermentation Chinese medicine, preparing Chinese medicine microbe leaven.
The content of the invention
It is an object of the invention to provide a kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application.
The present invention provides a kind of antibacterial Chinese medicine microbial fermentation preparation, it is using Chinese medicine as raw material, through lactobacterium acidophilus
It is prepared with Pediococcus pentosaceus co-fermentation；The Chinese medicine is purslane, Fructus Forsythiae or Cortex Phellodendri.
Wherein, the Chinese medicine is purslane, Fructus Forsythiae or Cortex Phellodendri.
Wherein, the quantity of the lactobacterium acidophilus and Pediococcus pentosaceus ratio is 1:1.
Wherein, it is described per 1g Chinese medicines, using 0.6 × 108The bacterium fermentation of CFU.
Wherein, the preparation is that it is to be prepared by the following method：
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus are taken according to proportioning；
(2) Feedstock treating：Chinese medicine is crushed, adds the water or MRS culture mediums of 6 times of weight of Chinese medicine, is mixed, sterilizing, then
MRS culture mediums are added, addition is 20 times of v/w of Chinese medicine, obtains material liquid；
(3) bacteria suspension is prepared：Respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, clump count, which is made, is
108The bacteria suspension of CFU/ml；
(4) ferment：Two kinds of bacteria suspensions of step (3) are added in the material liquid of step (2) at the same time, two kinds of bacteria suspensions plus
Enter 0.3 times of v/w that amount is Chinese medicine, 10h is cultivated under the conditions of 37 DEG C, to obtain the final product.
Wherein, the formulation is the preparation through gastrointestinal administration.Preferably, the preparation be oral liquid, tablet, pill,
Granule or capsule.
Present invention also offers the preparation method of foregoing Chinese medicine microbial fermentation preparation, it includes following operating procedure：
(1) Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus are taken according to proportioning；
(2) Feedstock treating：Chinese medicine is crushed, adds the water or MRS culture mediums of 6 times of weight of Chinese medicine, is mixed, sterilizing, then
MRS culture mediums are added, addition is 20 times of v/w of Chinese medicine, obtains material liquid；
(3) bacteria suspension is prepared：Respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, clump count, which is made, is
108The bacteria suspension of CFU/ml；
(4) ferment：Two kinds of bacteria suspensions of step (3) are added in the material liquid of step (2) at the same time, two kinds of bacteria suspensions plus
Enter 0.3 times of v/w that amount is Chinese medicine, 10h is cultivated under the conditions of 37 DEG C, to obtain the final product.
Present invention also offers foregoing Chinese medicine microbial fermentation preparation in the medicine or feed addictive for preparing antibacterial
Purposes.
Wherein, the medicine of the antibacterial or feed addictive are the medicines of anti-Escherichia coli and/or staphylococcus aureus
Thing or feed addictive.
Present invention also offers use of the foregoing Chinese medicine microbial fermentation preparation in the herbal medicine for preparing treatment grice diarrhoea
On the way.
Chinese medicine microbe leaven of the present invention can effectively treat grice diarrhoea, and positive effect is better than antibiotic, and secondary work
With small, can be used with substitute antibiotics, meanwhile, Chinese medicine microbe leaven of the present invention, to clinical pathogen Escherichia coli and gold
Staphylococcus aureus have obvious inhibitory action, and effect is superior to bacterium and Chinese medicine is used alone, and potential applicability in clinical practice is excellent.
Embodiment with reference to embodiments is again described in further detail the above of the present invention.But
The scope that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following example.The above-mentioned technological thought of the present invention is not being departed from
In the case of, the various replacements or change made according to ordinary skill knowledge and customary means, should all be included in the present invention
In the range of.
Embodiment
The preparation of the Chinese medicine microbial fermentation preparation of the present invention of embodiment 1
1.1 experiment material
1.1.1 bacterial strain：Pediococcus pentosaceus (deposit number), lactobacillus acidophilus (deposit number), E.coli K88, K99,987P reference culture, staphylococcus aureus is by Chinese industrial microbial bacteria
Kind preservation administrative center (CICC) purchase.
1.1.2 culture medium.1. MRS fluid nutrient mediums：For cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, ferment
Female cream 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate
0.25g, distilled water add to 1000ml, adjust pH to 6.0.2. MRS solid mediums：For cultivating lactobacillus acidophilus.What is made
The agar of 1.5%-2.0% is added in ship s fluid nutrient mediums.3. beef-protein medium：Bring enterobacteria and golden yellow up
Staphylococcus.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water add to 1000ml, adjust pH extremely
7.2-7.6。
Chinese medicine：Purslane
1.2 experimental method
1.2.1 seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are single respectively in the suspension that MRS slant mediums produce
Solely expand and be incubated in MRS meat soups, 37 DEG C of constant temperature incubation 48h, are made clump count as 1 × 108The seed liquor of CFU/mL.
Herb fermenting：After purslane section is crushed, 5g is taken in the triangular flask of 50mL, adds 30mL distilled water, 121 DEG C
Sterilize 20min, be added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then add lactobacillus acidophilus and
Pediococcus pentosaceus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
The preparation of the Chinese medicine microbial fermentation preparation of the present invention of embodiment 2
1.1 experiment material
1.1.1 bacterial strain：Pediococcus pentosaceus (deposit number), lactobacillus acidophilus (deposit number), E.coli K88, K99,987P reference culture, staphylococcus aureus is by Chinese industrial microbial bacteria
Kind preservation administrative center (CICC) purchase.
1.1.2 culture medium.1. MRS fluid nutrient mediums：For cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, ferment
Female cream 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate
0.25g, distilled water add to 1000ml, adjust pH to 6.0.2. MRS solid mediums：For cultivating lactobacillus acidophilus.What is made
The agar of 1.5%-2.0% is added in ship s fluid nutrient mediums.3. beef-protein medium：Bring enterobacteria and golden yellow up
Staphylococcus.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water add to 1000ml, adjust pH extremely
7.2-7.6。
Chinese medicine：Fructus Forsythiae
1.2 experimental method
1.2.1 seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are single respectively in the suspension that MRS slant mediums produce
Solely expand and be incubated in MRS meat soups, 37 DEG C of constant temperature incubation 48h, are made clump count as 1 × 108The seed liquor of CFU/mL.
Herb fermenting：After Fructus Forsythiae section is crushed, 5g is taken to add 30mL distilled water, 121 DEG C go out in the triangular flask of 50mL
Bacterium 20min, is added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then adds lactobacillus acidophilus and penta
Sugar-tablet coccus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
The preparation of the Chinese medicine microbial fermentation preparation of the present invention of embodiment 3
1.1 experiment material
1.1.1 bacterial strain：Pediococcus pentosaceus (deposit number), lactobacillus acidophilus (deposit number), E.coli K88, K99,987P reference culture, staphylococcus aureus is by Chinese industrial microbial bacteria
Kind preservation administrative center (CICC) purchase.
1.1.2 culture medium.1. MRS fluid nutrient mediums：For cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, ferment
Female cream 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate
0.25g, distilled water add to 1000ml, adjust pH to 6.0.2. MRS solid mediums：For cultivating lactobacillus acidophilus.What is made
The agar of 1.5%-2.0% is added in ship s fluid nutrient mediums.3. beef-protein medium：Bring enterobacteria and golden yellow up
Staphylococcus.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water add to 1000ml, adjust pH extremely
7.2-7.6。
Chinese medicine：Cortex Phellodendri
1.2 experimental method
1.2.1 seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are single respectively in the suspension that MRS slant mediums produce
Solely expand and be incubated in MRS meat soups, 37 DEG C of constant temperature incubation 48h, are made clump count as 1 × 108The seed liquor of CFU/mL.
Herb fermenting：After Cortex Phellodendri section is crushed, 5g is taken to add 30mL distilled water, 121 DEG C go out in the triangular flask of 50mL
Bacterium 20min, is added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then adds lactobacillus acidophilus and penta
Sugar-tablet coccus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
Beneficial effects of the present invention are illustrated below by way of test example.
The Antibacterial Activity of the Chinese medicine microbial fermentation preparation of the present invention of experimental example 1
1.1 experiment material
1.1.1 bacterial strain：Pediococcus pentosaceus (deposit number), lactobacillus acidophilus (deposit number), E.coli K88, K99,987P reference culture, staphylococcus aureus is by Chinese industrial microbial bacteria
Kind preservation administrative center (CICC) purchase.
1.1.2 culture medium.1. MRS fluid nutrient mediums：For cultivating lactobacillus acidophilus.Peptone 10g, beef extract 10g, ferment
Female cream 5g, K2HP042g, sodium acetate 5g, ammonium citrate 2g, glucose 20g, Tween-80 1mL, magnesium sulfate 0.58g, manganese sulfate
0.25g, distilled water add to 1000ml, adjust pH to 6.0.2. MRS solid mediums：For cultivating lactobacillus acidophilus.What is made
The agar of 1.5%-2.0% is added in ship s fluid nutrient mediums.3. beef-protein medium：Bring enterobacteria and golden yellow up
Staphylococcus.Beef extract 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g, distilled water add to 1000ml, adjust pH extremely
7.2-7.6。
Chinese medicine：Purslane, Fructus Forsythiae, Cortex Phellodendri
1.2 experimental method
1.2.1 the preparation of experiment material
Packet：It is divided into four groups, I Chinese medicine+water+bacterium；II Chinese medicine+culture medium+bacterium；III Chinese medicine+culture medium；IV culture medium+bacterium.
Group I：Chinese medicine microbial fermentation preparation 1 of the present invention
Seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are individually expanded in the suspension that MRS slant mediums produce
It is incubated in MRS meat soups greatly, 37 DEG C of constant temperature incubation 48h, clump count is made as 1 × 108The seed liquor of CFU/mL.
Herb fermenting：By purslane, after Fructus Forsythiae or Cortex Phellodendri section crushing, 5g is taken to add 30mL in the triangular flask of 50mL
Distilled water, 121 DEG C of sterilizing 20min, is added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then adds
Lactobacillus acidophilus and Pediococcus pentosaceus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
Experimental group II：Chinese medicine microbial fermentation preparation 2 of the present invention
Seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are individually expanded in the suspension that MRS slant mediums produce
It is incubated in MRS meat soups greatly, 37 DEG C of constant temperature incubation 48h, clump count is made as 108The seed liquor of CFU/mL.
Herb fermenting：By purslane, after Fructus Forsythiae or Cortex Phellodendri section crushing, 5g is taken to be added in the triangular flask of 50mL
30mLMRS culture mediums, 121 DEG C of sterilizing 20min, are added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then
Add lactobacillus acidophilus and Pediococcus pentosaceus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
Experimental group III：Control 1
By purslane, after Fructus Forsythiae or Cortex Phellodendri section crushing, 5g is taken to add 30mLMRS culture mediums in the triangular flask of 50mL,
121 DEG C of sterilizing 20min, are added in the triangular flask (250mL) of the MRS culture mediums containing 100mL.
Experimental group IV：Control 2
Seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are individually expanded in the suspension that MRS slant mediums produce
It is incubated in MRS meat soups greatly, 37 DEG C of constant temperature incubation 48h, clump count is made as 108The seed liquor of CFU/mL.Normal fermentation：Take
In the triangular flask (250mL) of the MRS culture mediums of 100mL, then add lactobacillus acidophilus and Pediococcus pentosaceus seed liquor is each
1.5mL, 37 DEG C of constant temperature incubation 10d.
Write according to your material, whether please verify accurate.
1.2.2 sample treatment：After fermentate and each group control the culture 10d of different Chinese medicines, filter paper filtering, filtrate concentration,
It is settled to 5mL.100 μ L are directly taken to carry out Activity determination during use.
1.2.3 bacteriostatic experiment (using Odontothrips loti)：Double-deck agar plate is used as indicator bacteria tablet, bottom adds 15mL
2% nutrient agar.After bottom cooling, the Oxford cup that several outside diameters are 6mm is put into, takes 1mL pathogenic bacteria bacteria suspensions to be added to
The agar concentration that 15mL is cooled to 45 DEG C or so is in 1% culture medium, and rapid mixing, is poured on nutrient agar, cools down
Oxford cup is taken out with aseptic nipper afterwards, that is, forms some holes, injects 200 μ L fermented supernatant fluids, 37 DEG C of constant temperature respectively inwards
24h is cultivated, observes inhibition zone size around hole, and transparent loop diameter is measured with vernier caliper.Observe the sample of each group processing
The bacteriostatic experiment that product are done is as a result, the size for measuring inhibition zone for having inhibition zone to produce, no inhibition zone generation are thought at this
Reason does not have bacteriostasis under this dosage, and antibacterial circle diameter thinks only Weak activity less than 7mm's.
1.3 results and analysis
Fungistatic effect of the Chinese medicine microecological preparation of the present invention to pathogenic bacteria
Note：- there is no inhibitory action.
As can be seen from the above table, relative to blank control, Chinese medicine microbial fermentation preparation 1 and 2 (group I and group II) of the present invention
Escherichia coli and staphylococcus aureus can effectively be suppressed；In addition, pure Chinese medicine purslane, Fructus Forsythiae, Cortex Phellodendri with same dose
(group III) and pure probiotics normal fermentation (group IV) are compared, and the inhibition zone of Chinese medicine microbial fermentation preparation of the present invention is substantially more
Greatly, fungistatic effect is substantially more preferable.
The experiment results show that Chinese medicine microbial fermentation preparation of the present invention is to clinical bacteria Escherichia coli and golden yellow grape
The inhibitory activity of coccus is excellent, available for treating both bacterial infectious diseases.
The effect of the Chinese medicine microbial fermentation preparation treatment grice diarrhoea of the present invention of experimental example 2
The preparation of 1 Chinese medicine microbial fermentation preparation of the present invention
Seed liquor：Lactobacterium acidophilus and Pediococcus pentosaceus are individually expanded in the suspension that MRS slant mediums produce
It is incubated in MRS meat soups greatly, 37 DEG C of constant temperature incubation 48h, clump count is made as 108The seed liquor of CFU/mL.
Herb fermenting：By purslane, after Fructus Forsythiae or Cortex Phellodendri section crushing, 5g is taken to add 30mL in the triangular flask of 50mL
Distilled water, 121 DEG C of sterilizing 20min, is added in the triangular flask (250mL) of the MRS culture mediums containing 100mL, then adds
Lactobacillus acidophilus and Pediococcus pentosaceus seed liquor each 1.5mL, 37 DEG C of constant temperature incubation 10d.
Control is used as using aureomycin.
2 test methods
The pig farm that yellowish-white dysentery occurs in Yaan is tested, and piglet of this in delivery room in December shows as violent abdomen
Rush down, excreta stench, yellowly paste or water sample, the escherichia coli abdomen of piglet is diagnosed as through clinical diagnosis and test in laboratory
Rush down.The piglet that diarrhea occurs is grouped by 30 every group, is always divided into 4 groups, respectively the abdomen of observation experiment group and control group
Situation, and effective percentage of the record after 7 days are rushed down, does not have group to randomly select piglet 3 after treatment end, separation jejunal segment takes its jejunum to glue
Film, with the content of its SIgA, IL-2 of Elisa kit measurements.
3 results and analysis
The experimental results are shown inthe following table：
As can be seen from the above table, after using three kinds of Chinese medicine microbial fermentation preparation treatments of the present invention, grice diarrhoea is obvious
Declining, the content of Jejunum of Piglets mucous membrane proinflammatory cytokine IL-2 significantly reduces, and the amount of the IgA antibody of secreting type dramatically increases,
Illustrate that three kinds of Chinese medicine microbial fermentation preparations of the invention can effectively treat grice diarrhoea, and positive effect is better than positive drug
Aureomycin.
Meanwhile the outcome of Chinese medicine microbial fermentation preparation of the present invention is substantially better than aureomycin, after aureomycin treatment
The piglet later stage becomes cad pig, and the piglet after Chinese medicine microorganism formulation of the present invention treatment then with normal piglets no significant difference.
To sum up, Chinese medicine microbe leaven of the present invention can effectively treat grice diarrhoea, and positive effect is better than antibiotic, and
Small side effects, can be used with substitute antibiotics, meanwhile, Chinese medicine microbe leaven of the present invention, to clinical pathogen Escherichia coli
There is obvious inhibitory action with staphylococcus aureus, effect is superior to bacterium and Chinese medicine is used alone, and potential applicability in clinical practice is excellent
It is good.
Claims (6)
- A kind of 1. antibacterial Chinese medicine microbial fermentation preparation, it is characterised in that：It is using Chinese medicine as raw material, through lactobacterium acidophilus and penta Sugar-tablet coccus co-fermentation is prepared；The Chinese medicine is purslane, Fructus Forsythiae or Cortex Phellodendri；The quantity of the lactobacterium acidophilus and Pediococcus pentosaceus ratio is 1:1；Per 1g Chinese medicines, using 0.6 × 108The bacterium fermentation of CFU；Wherein, preparation is to be prepared by the following method：（1）Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus are taken according to proportioning；（2）Feedstock treating：Chinese medicine is crushed, adds the water or MRS culture mediums of 6 times of weight of Chinese medicine, is mixed, sterilizing, adds MRS culture mediums, addition are 20 times of v/w of Chinese medicine, obtain material liquid；（3）Prepare bacteria suspension：Respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, it is 10 that clump count, which is made,8CFU/ml Bacteria suspension；（4）Fermentation：By step（3）Two kinds of bacteria suspensions add step at the same time（2）Material liquid in, the addition of two kinds of bacteria suspensions Be 0.3 times of v/w of Chinese medicine, 10h cultivated under the conditions of 37 DEG C, to obtain the final product.
- 2. preparation according to claim 1, it is characterised in that：Formulation is the preparation through gastrointestinal administration.
- 3. preparation according to claim 2, it is characterised in that：The preparation be oral liquid, tablet, pill, granule or Capsule.
- 4. the preparation method of Chinese medicine microbial fermentation preparation described in claim 1 ~ 3 any one, it is characterised in that：It is included such as Lower operating procedure：（1）Chinese medicine, lactobacterium acidophilus and Pediococcus pentosaceus are taken according to proportioning；（2）Feedstock treating：Chinese medicine is crushed, adds the water or MRS culture mediums of 6 times of weight of Chinese medicine, is mixed, sterilizing, adds MRS culture mediums, addition are 20 times of v/w of Chinese medicine, obtain material liquid；（3）Prepare bacteria suspension：Respectively by lactobacterium acidophilus and Pediococcus pentosaceus activation culture, it is 10 that clump count, which is made,8CFU/ml Bacteria suspension；（4）Fermentation：By step（3）Two kinds of bacteria suspensions add step at the same time（2）Material liquid in, the addition of two kinds of bacteria suspensions Be 0.3 times of v/w of Chinese medicine, 10h cultivated under the conditions of 37 DEG C, to obtain the final product.
- 5. Chinese medicine microbial fermentation preparation described in claim 1 ~ 3 any one is preparing the medicine or feed addictive of antibacterial In purposes, wherein, the medicine or feed addictive of the antibacterial are the medicines of anti-Escherichia coli and/or staphylococcus aureus Thing or feed addictive.
- 6. Chinese medicine microbial fermentation preparation described in claim 1 ~ 3 any one is in the herbal medicine for preparing treatment grice diarrhoea Purposes.
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