CN104435046A - Fermented medicine for treating piglet's yellow-white dysentery and preparation method of fermented medicine - Google Patents
Fermented medicine for treating piglet's yellow-white dysentery and preparation method of fermented medicine Download PDFInfo
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- 239000003814 drug Substances 0.000 title claims abstract description 33
- 208000001848 dysentery Diseases 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title description 13
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 6
- 239000002054 inoculum Substances 0.000 claims abstract description 5
- 238000000855 fermentation Methods 0.000 claims description 25
- 230000004151 fermentation Effects 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 18
- 239000012467 final product Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 7
- 238000001035 drying Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 abstract description 2
- 240000000724 Berberis vulgaris Species 0.000 abstract 1
- 235000016068 Berberis vulgaris Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 241000411851 herbal medicine Species 0.000 description 5
- 239000003094 microcapsule Substances 0.000 description 5
- 239000008738 huangbai Substances 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000009413 Chuanxinlian Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000005485 electric heating Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000825 pharmaceutical preparation Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010048768 Dermatosis Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002052 anaphylactic effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 208000030172 endocrine system disease Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- Health & Medical Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a fermented medicine for treating piglet's yellow-white dysentery. The fermented medicine is prepared by virtue of the following steps: (1) taking 2 parts by weight of parslane herb and 1 part of barberry root, drying, crushing and sieving by use of a 60-mesh sieve, thereby obtaining powder; (2) adding water 10 times the powder by volume, sterilizing, inoculating with a bacillus subtilis liquor by the inoculum size of 10%, culturing for 5 days at 37 DEG C under the condition of 200r/min, centrifuging and filtering to obtain the filtrate; and (3) performing spray-drying on the filtrate, thereby obtaining the fermented medicine. The invention also discloses a method for preparing the fermented medicine for treating piglet's yellow-white dysentery. The fermented medicine has an excellent effect on treatment of piglet's yellow-white dysentery and bright clinical application prospect.
Description
Technical field
The present invention relates to a kind of fermentation medicine for the treatment of yellow and white dysentery of piglet and preparation method thereof.
Background technology
Theory of Chinese medical science is thought, mostly yellow and white dysentery of piglet is, with the damp and hot heresy of carcass impression, to injure the intestines and stomach, invade in epidemic disease poison, cause intestinal clear QI being mixed up with turbid QI, the wet bet of water and rawly to have loose bowels.Doctor trained in Western medicine theory is thought to cause because of coli-infection, and in therefore combining, veterinary is theoretical, and first-selected heat clearing and damp drying, has the Chinese medicine of certain fungistatic effect in vitro as prescription foundation, selects Chinese medicine to comprise Radix Berberidis and Herba Portulacae two taste.
Herba Portulacae is the dry aerial parts of portulacaceous plant Herba Portulacae, sour, cold.Return liver, large intestine channel.There is heat-clearing and toxic substances removing, effect of eliminating pathogenic heat from blood to cure dysentery.As the Chinese medicine of China, Herba Portulacae pharmacologically have anti-mattress, blood fat reducing, blood sugar lowering, antitumor, anti-aging, strengthen immunity etc. effect, then the common diseases such as intestinal tract disease, gynaecopathia, dermatosis, endocrinopathy, anaphylactic disease, liver and gall diseases, urinary disorders can be treated in clinical practice, there is higher medical value, very worth further research, development and utilization.
Utilize fermentable as Chinese herbal medicine concocting method, have the history of several thousand in China.Chinese herbal medicine by fermentation after, by the digest and decompose of probiotics, the release that effective ingredient in medicine can be maximum, and Chinese herbal medicine is through the pretreatment of microorganism, some macromolecular components not easily absorbed can be converted into micromolecule, are convenient to body and absorb, rapid performance medicine usefulness, thus heighten the effect of a treatment.Fermentable take Chinese herbal medicine as substrate, can produce new active substance in the growth and breeding stage, but also can synthesize multivitamin, and make effective ingredient in the Chinese herbal medicine after fermenting abundanter, action effect is more remarkable, and using value is stronger.
Publication number be 103127198A application discloses the pharmaceutical preparation prepared for raw material with Herba Portulacae and Radix Berberidis, wherein, the microcapsule formulation curative effect of preparation is best, and clinical cure rate is 86%, is further improved.
Summary of the invention
The invention provides a kind of fermentation medicine for the treatment of yellow and white dysentery of piglet and preparation method thereof.
The present invention treats the fermentation medicine of yellow and white dysentery of piglet, and it is prepared by the following method:
(1) get Herba Portulacae 2 weight portion, Radix Berberidis 1 weight portion, dry, pulverize, cross 60 mesh sieves, obtain powder;
(2) add the water of powder 10 times of volumes, sterilizing, according to the inoculum concentration inoculation bacillus subtilis bacterium liquid of 10%, in 200r/min, at 37 DEG C, cultivate 5d, centrifugal, filter, obtain filtrate;
(3) filtrate makes various oral formulations, comprises for powder, tablet, pill, capsule, microcapsule, granule, medicinal cake or liquid preparation.
The present invention prepares the method for the fermentation medicine for the treatment of yellow and white dysentery of piglet, and it comprises the steps:
(1) get Herba Portulacae 2 weight portion, Radix Berberidis 1 weight portion, dry, pulverize, cross 60 mesh sieves, obtain powder;
(2) add the water of powder 10 times of volumes, sterilizing, according to the inoculum concentration inoculation bacillus subtilis bacterium liquid of 10%, in 200r/min, at 37 DEG C, cultivate 5d, centrifugal, filter, obtain filtrate;
(3) by filtrate spraying dry, to obtain final product.
In step (1), oven dry is 60 DEG C of oven dry.
In step (2), described sterilizing is 121 DEG C of autoclaving 20min.
In step (2), in described bacillus subtilis bacterium liquid, the concentration of bacillus subtilis is 1 × 10
7-1 × 10
9cfu/ml.
In step (2), described bacillus subtilis bacterium liquid is prepared as follows: get bacillus subtilis, be inoculated in liquid broth, cultivates 24h, to obtain final product in 150r/min, at 37 DEG C.
To sum up, the present invention for crude drug, adopts pharmaceutical preparation prepared by fermentation process of the present invention with Radix Berberidis and Herba Portulacae, and the clinical efficacy for the treatment of yellow and white dysentery of piglet is excellent, and potential applicability in clinical practice is excellent.
Below by detailed description of the invention, the present invention is described in further details, but be not limitation of the present invention, according to foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, not departing under the present invention's above-mentioned basic fundamental thought prerequisite, the amendment of other various ways, replacement or change can also be made.
Detailed description of the invention
Bacillus subtilis mycopowder, purchased from China General Microbiological culture presevation administrative center, numbering 1.1661.
Liquid broth, often liter comprises following composition: peptone 10g Carnis Bovis seu Bubali cream 3g sodium chloride 5g distilled water 1000mL, pH 7.4.
The preparation method of embodiment 1 medicine of the present invention
Strain activation and culture: aseptically, the distilled water of first sterilizing of being acted by bacillus subtilis mycopowder fully dissolves, and inoculation bacterium solution, on agar culture medium, is placed in electro-heating standing-temperature cultivator and activates 12h at 37 DEG C.Picking colony is transferred in the 250mL triangular flask that 100mL liquid broth is housed, and covers sealed membrane, is placed in electric heating constant temperature shaken cultivation case in 150r/min, cultivates 24h at 37 DEG C, and obtaining bacterial concentration is 1 × 10
7-1 × 10
9the bacillus subtilis bacterium liquid of cfu/ml, for subsequent use.
Fermentation: get Herba Portulacae 2 parts, Radix Berberidis 1 part, 60 DEG C of oven dry, pulverizes, and crosses 60 mesh sieves, for subsequent use.Get the water that above-mentioned powder adds 10 volumes, load triangular flask according to 30% loading amount, stir, at 121 DEG C of autoclaving 20min, aseptically adding 10% (V/V) aforementioned bacterial concentration after cooling is 10
7-9the bacillus subtilis bacterium liquid of cfu/ml, is placed in electric heating constant temperature shaken cultivation case, in 200r/min, cultivates 5d at 37 DEG C, centrifugal filtration after fermentation ends, filtrate spraying dry, obtains fermentation preparation of the present invention.
Below by the mode of experimental example, beneficial effect of the present invention is described:
Experimental example 1 the present invention medicine that ferments compares with the drug effect of existing medicine
One, experimental technique
Gather the sick pig of experiment pig farm, the ground such as Wenjiang, Sichuan, Jianyang natural occurrence, the sick pig totally 150 of HUANGBAI(sic) dysentery is diagnosed as through epidemiology, clinical symptoms, test in laboratory, be divided into 3 groups at random, often organize 50, namely blank group, fermentation preparation treatment group of the present invention, Radix Berberidis and Herba Portulacae microcapsule formulation matched group, CHUANXINLIAN ZHUSHEYE matched group.
Blank group: diet;
Fermentation preparation treatment group of the present invention: get the embodiment of the present invention 1 prepare for three days on end by 2%/kg spice feeding;
2%/kg: be meant to the feedstuff that weight is 1kg, adds the fermentation preparation of the present invention of food 2% weight.
The microcapsule formulation matched group of Radix Berberidis and Herba Portulacae: 0.2mL/kg dosage administered intramuscular.
CHUANXINLIAN ZHUSHEYE matched group: each 5-10ml/ head intramuscular injection, every day 2 times.
Observe and add up the mortality rate, the mental status, clinical symptoms etc. that 3 days respectively organize laboratory animal.
Index of assessment of curative effect:
Cure: the HUANGBAI(sic) dysentery transference cure of clinical appearance, perianal, without dirt, observes 3 days nothings phenomenon repeatedly.
Effective: the HUANGBAI(sic) dysentery symptom of clinical appearance obtains partial rcsponse, feces becomes dry, without the phenomena of mortality.
Invalid: the HUANGBAI(sic) dysentery symptom of clinical appearance is not improved, and occurs the piglet phenomena of mortality.
Two, experimental result
Experimental result is as shown in table 1:
Table 1 clinical test results
As shown in table 1, compare with the microcapsule formulation of Herba Portulacae with existing Radix Berberidis, the cure rate of this fermentation preparation significantly improves.
Experimental result illustrates, with Radix Berberidis and Herba Portulacae for crude drug, the preparation adopting fermentation process of the present invention to prepare, more effectively can treat yellow and white dysentery of piglet.
Claims (10)
1. treat a fermentation medicine for yellow and white dysentery of piglet, it is characterized in that: it is prepared by the following method:
(1) get Herba Portulacae 2 weight portion, Radix Berberidis 1 weight portion, dry, pulverize, cross 60 mesh sieves, obtain powder;
(2) add the water of powder 10 times of volumes, sterilizing, according to the inoculum concentration inoculation bacillus subtilis bacterium liquid of 10% (V/V), cultivate 5d in 200r/min, at 37 DEG C, centrifugal, filter, obtain filtrate;
(3) by filtrate spraying dry, to obtain final product.
2. fermentation medicine according to claim 1, is characterized in that: in step (1), and oven dry is 60 DEG C of oven dry.
3. fermentation medicine according to claim 1, is characterized in that: in step (2), and described sterilizing is 121 DEG C of sterilizing 20min.
4. fermentation medicine according to claim 1, is characterized in that: in step (2), and in described bacillus subtilis bacterium liquid, the concentration of bacillus subtilis is 1 × 10
7-1 × 10
9cfu/ml.
5. fermentation medicine according to claim 4, is characterized in that: described bacillus subtilis bacterium liquid is prepared as follows: get bacillus subtilis, be inoculated in liquid broth, cultivates 24h, to obtain final product in 150r/min, at 37 DEG C.
6. prepare a method for the fermentation medicine for the treatment of yellow and white dysentery of piglet, it is characterized in that: it comprises the steps:
(1) get Herba Portulacae 2 weight portion, Radix Berberidis 1 weight portion, dry, pulverize, cross 60 mesh sieves, obtain powder;
(2) add the water of powder 10 times of volumes, sterilizing, according to the inoculum concentration inoculation bacillus subtilis bacterium liquid of 10%, in 200r/min, at 37 DEG C, cultivate 5d, centrifugal, filter, obtain filtrate;
(3) by filtrate spraying dry, to obtain final product.
7. fermentation medicine according to claim 6, is characterized in that: in step (1), and oven dry is 60 DEG C of oven dry.
8. fermentation medicine according to claim 6, is characterized in that: in step (2), and described sterilizing is 121 DEG C of autoclaving 20min.
9. fermentation medicine according to claim 6, is characterized in that: in step (2), and in described bacillus subtilis bacterium liquid, the concentration of bacillus subtilis is 1 × 10
7-1 × 10
9cfu/ml.
10. fermentation medicine according to claim 9, is characterized in that: described bacillus subtilis bacterium liquid is prepared as follows: get bacillus subtilis, be inoculated in liquid broth, cultivates 24h, to obtain final product in 150r/min, at 37 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410787949.0A CN104435046B (en) | 2014-12-17 | 2014-12-17 | A kind of fermentation medicine for treating piglet yellow-white dysentery and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410787949.0A CN104435046B (en) | 2014-12-17 | 2014-12-17 | A kind of fermentation medicine for treating piglet yellow-white dysentery and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104435046A true CN104435046A (en) | 2015-03-25 |
| CN104435046B CN104435046B (en) | 2017-11-24 |
Family
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|---|---|---|---|
| CN201410787949.0A Active CN104435046B (en) | 2014-12-17 | 2014-12-17 | A kind of fermentation medicine for treating piglet yellow-white dysentery and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855684A (en) * | 2015-04-13 | 2015-08-26 | 成都乾坤动物药业有限公司 | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206692A (en) * | 2011-05-06 | 2011-10-05 | 成都乾坤动物药业有限公司 | Process for increasing extraction amount of baicilin by using traditional Chinese medicine fermentation technology |
| CN103127198A (en) * | 2013-03-01 | 2013-06-05 | 成都乾坤动物药业有限公司 | Combined drug for treating piglet's yellow-white dysentery |
-
2014
- 2014-12-17 CN CN201410787949.0A patent/CN104435046B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206692A (en) * | 2011-05-06 | 2011-10-05 | 成都乾坤动物药业有限公司 | Process for increasing extraction amount of baicilin by using traditional Chinese medicine fermentation technology |
| CN103127198A (en) * | 2013-03-01 | 2013-06-05 | 成都乾坤动物药业有限公司 | Combined drug for treating piglet's yellow-white dysentery |
Non-Patent Citations (1)
| Title |
|---|
| 陈娟娟: "发酵中草药在动物生产方面的应用展望", 《山西农业(畜牧兽医)》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104855684A (en) * | 2015-04-13 | 2015-08-26 | 成都乾坤动物药业有限公司 | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof |
| CN104855684B (en) * | 2015-04-13 | 2018-05-01 | 成都乾坤动物药业有限公司 | A kind of antibacterial Chinese medicine microbial fermentation preparation and its preparation method and application |
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Address after: No. 259, Middle Section of Jinfu Road, Wenjiang District, Chengdu City, Sichuan Province, 611130 Patentee after: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL CO.,LTD. Address before: 611130 no.259 Jinfu Road, Chengdu cross strait science and Technology Industrial Development Park, Wenjiang District, Chengdu City, Sichuan Province Patentee before: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL Co.,Ltd. |