CN102366021A - Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics - Google Patents
Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics Download PDFInfo
- Publication number
- CN102366021A CN102366021A CN2011102764143A CN201110276414A CN102366021A CN 102366021 A CN102366021 A CN 102366021A CN 2011102764143 A CN2011102764143 A CN 2011102764143A CN 201110276414 A CN201110276414 A CN 201110276414A CN 102366021 A CN102366021 A CN 102366021A
- Authority
- CN
- China
- Prior art keywords
- parts
- inoculated
- seed
- culture medium
- chinese medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a manufacturing method of a poultry feed additive containing traditional Chinese medicinal activity probiotics, comprising the following steps of: carrying out single stain quantity enlarge culture on the original strain of every poultry activity probiotic; mixing equal weight first-level seed liquids of each poultry activity probiotic, inoculating to a medium in a seed jar for mixing and culturing of a plurality of strains, and inoculating into a traditional Chinese medicinal liquid medium for the fermentation of the traditional Chinese medicine so as to prepare the poultry feed additive containing traditional Chinese medicinal activity probiotics. The embodiment provided by the invention can be implemented to greatly raise the strain activity of activity probiotics in the poultry feed additive and the colonization performance in intestinal tract, make the prepared poultry feed additive rich in traditional Chinese medicinal components and make the active components with pharmacological effects in the traditional Chinese medicinal components to fully perform, so as to raise the poultry feed additive's body strengthening and disease treating performance and growth promotion performance. Furthermore, antibiotics are completely replaced.
Description
Technical field
The present invention relates to the animal and fowl fodder field, relate in particular to a kind of manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic.
Background technology
In recent years, the communicable disease of poultry was concentrated outburst, and poultry farming is suffered heavy losses.In order to strengthen the outburst of poultry physique, raising poultry resistance against diseases, the extensive communicable disease of minimizing, many poultry producers have adopted to the antibiotic feeding patterns of the long-term feeding of poultry one after another.Antibiotic has kill bacteria and the effect that suppresses pathogenic microorganisms, and therefore this feeding patterns has played certain protection effect in actual production.But; These antibiotic can get into human body by the edible various poultry meat goods of Along with people's, and this has just caused antibiotic abuse indirectly, might cause multiple drug induced disease; Even can produce new " super drug-fast bacteria ", thereby human life and health in this feeding patterns serious threat.
For fear of or reduce the poultry of being in and feed the medium-term and long-term antibiotic that uses of process, poultry is arisen at the historic moment with microbial forage additive.So-called poultry is the active probiotic preparation of processing through special process to the useful and harmless active probiotic of poultry with microbial forage additive.Poultry is added auxiliary material with microbial forage additive as a kind of functional poultry feed; Have the advantages that to have no side effect, have no drug resistance, do not have pathogenicity, can regulate poultry intestinal microecology balance, raising poultry growth rate, raising efficiency of feed utilization, the breeding that suppresses the harmful intestinal tract microorganism, the raising poultry general level of the health, strengthen the every household immunity of fowl.At present; In existing poultry farming, common poultry has nearly hundred kinds in lactobacillus preparation (comprising lactobacterium acidophilus, bifid lactobacillus, streptococcus fecalis etc.), bacillus preparation (comprising hay bacillus, bacillus cereus, lichens bacillus etc.), fungi preparation (comprising aspergillus oryzae etc.), saccharomycete preparation (comprising brewer's yeast etc.) etc. with the kind of microbial forage additive.Existing poultry with the microbial forage additive production process in; Only use protein substance and/or glucide culture medium mostly as active probiotic; Though bacterial strain quantitatively can satisfy the demands; But the activity of most of bacterial strains is not high, the field planting performance in enteron aisle a little less than, so active probiotic as animal and fowl fodder the effect that should bring into play receive very big restriction; Simultaneously, active probiotic only can strengthen the physique of poultry to a certain extent, can't strengthen the disease resistance ability of poultry, does not more possess the disease treatment effect, and therefore, existing family poultry microbial forage additive can not replace antibiotic use fully.
Summary of the invention
The embodiment of the invention provides a kind of manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic; So that significantly improve the active and field planting performance in enteron aisle of the bacterial strain of active probiotic in the livestock fodder additives; And make the livestock fodder additives that makes be rich in traditional Chinese medicine ingredients; The active component that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to, performance and growth promotion performance thereby the strong body of raising livestock fodder additives is cured the disease, and then replace antibiotic use fully.
The objective of the invention is to realize through following technical scheme:
A kind of manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic comprises:
Steps A: choose the multiple poultry that equates bacterial strain quantity former bacterial strain, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity expansion cultivation with the former bacterial strain of active probiotic, thereby obtain the primary seed solution of every kind of poultry with active probiotic with active probiotic;
Wherein, described multiple poultry with active probiotic comprise bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast 、 Chan ?in Candida, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds;
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out the Chinese medicine fermentation in the Chinese medicine fluid nutrient medium, thereby make the livestock fodder additives that contains the Chinese medicine active probiotic;
Wherein, described Chinese medicine fluid nutrient medium comprises 5%～8% brown sugar, 2%～3% traditional Chinese medicinal components, 0.2%～0.3% potassium dihydrogen phosphate, 0.3%～0.5% yeast extract and the water of surplus; Described traditional Chinese medicinal components is made up of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, gynostemma pentaphylla: 60～80 parts, wilsonii: 60～80 parts,
The root of large-flowered skullcap: 50～80 parts, Radix Isatidis: 40～60 parts, the Chinese bulbul: 40～60 parts, honeysuckle: 40～60 parts,
Radix Angelicae Sinensis: 40～60 parts, hawthorn: 50～80 parts, dried orange peel: 50～80 parts, purslane: 40～60 parts,
Radix Glycyrrhizae: 30～50 parts.
Technical scheme by the invention described above provides can be found out; The manufacturing approach of the livestock fodder additives that contains the Chinese medicine active probiotic that the embodiment of the invention provides adopts kinds of traditional Chinese medicines is cultivated has good facilitation to poultry growth and resistance against diseases active probiotic; Thereby make the active component in the Chinese medicine give full play to pharmacological action; And the active probiotic that the activity that makes the active probiotic of being turned out is turned out far above ordinary culture medium has also strengthened the field planting ability of active probiotic in enteron aisle simultaneously; Because Chinese medicine and active probiotic have synergy to immune system, so the active probiotic that will breed with Chinese medicine can strengthen the immunocompetence of poultry greatly as the poultry feed additive.We cultivate active probiotic through modern microecology principle is combined with the traditional Chinese medical science with traditional Chinese medicinal components, thereby produce a kind of antibiotic no drug residue, prophyiaxis and promoting growth, poultry feed additive with low cost of replacing fully.
Description of drawings
In order to be illustrated more clearly in the technical scheme of the embodiment of the invention; The accompanying drawing of required use is done to introduce simply in will describing embodiment below; Obviously, the accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skill in the art; Do not paying under the capable prerequisite of creative work, can also obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is the schematic flow sheet of the manufacturing approach of the livestock fodder additives that contains the Chinese medicine active probiotic that the embodiment of the invention provided.
The specific embodiment
Following combines the accompanying drawing in the embodiment of the invention, and the technical scheme in the embodiment of the invention is carried out clear, intactly description, and obviously, described embodiment only is the present invention's part embodiment, rather than whole embodiment.Based on embodiments of the invention, those of ordinary skills belong to protection scope of the present invention not making the every other embodiment that is obtained under the creative work prerequisite.
At first, need to prove that part alleged in the present specification all is meant weight portion, make a detailed description in the face of the manufacturing approach of the livestock fodder additives that contains the Chinese medicine active probiotic that the embodiment of the invention provided down.
As shown in Figure 1, a kind of manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic specifically can may further comprise the steps:
Steps A: choose the multiple poultry that equates bacterial strain quantity former bacterial strain, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity expansion cultivation with the former bacterial strain of active probiotic, thereby obtain the primary seed solution of every kind of poultry with active probiotic with active probiotic;
Wherein, described multiple poultry with active probiotic comprise bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast 、 Chan ?in Candida, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds;
Particularly; Choose the former bacterial strain of the multiple poultry of equal bacterial strain quantity accordingly with active probiotic; And respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, can comprise with the primary seed solution of active probiotic thereby obtain every kind of poultry:
The former bacterial strain seed inclined-plane that (1) will separate the bacillus subtilis after purifying is inoculated in the triangular flask of bacillus subtilis bacterium culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bacillus subtilis; The bacillus subtilis bacterium culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; Wherein, the pH value of this culture medium should just can be inoculated in 7.0～7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bacillus subtilis is the bacillus subtilis of the bacillus subtilis inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (2) will separate the bafillus natto after purifying is inoculated in the triangular flask of bafillus natto culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bafillus natto; The bafillus natto culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; The pH value of this culture medium should just can be inoculated in 7.0～7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bafillus natto is the bafillus natto of the bafillus natto inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (3) will separate the bacillus licheniformis after purifying is inoculated in the triangular flask of bacillus licheniformis culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bacillus licheniformis; The bacillus licheniformis culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water; The pH value of this culture medium should just can be inoculated in 7.0～7.2 scope, to guarantee that bacterial strain activity and bacterial strain survival condition can not destroyed.Need to prove that the inoculative proportion of bacillus licheniformis is the bacillus licheniformis of the bacillus licheniformis inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (4) will separate the brewer's yeast after purifying is inoculated in the triangular flask of brewer's yeast culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of brewer's yeast; The brewer's yeast culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of brewer's yeast is the brewer's yeast of the brewer's yeast inoculation of medium 100mL of every 500mL.
And/or,
(5) will separate purify after De Chan ?the former bacterial strain seed inclined-plane of Candida be inoculated into Chan ?in the triangular flask of Candida culture medium, and shaken cultivation 48 hours, thus Huo De Chan ?the primary seed solution of Candida; Chan ?the Candida culture medium comprise 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove ， Chan ?the inoculative proportion of Candida be every 500mL De Chan ?Candida inoculation of medium 100mL De Chan ?Candida.
And/or,
The former bacterial strain seed inclined-plane that (6) will separate the aspergillus niger after purifying is inoculated in the triangular flask of aspergillus niger culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of aspergillus niger; The aspergillus niger culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of aspergillus niger is the aspergillus niger of the aspergillus niger inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (7) will separate the Rhizopus oryzae after purifying is inoculated in the triangular flask of Rhizopus oryzae culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of Rhizopus oryzae; The Rhizopus oryzae culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water.Need to prove that the inoculative proportion of Rhizopus oryzae is the Rhizopus oryzae of the Rhizopus oryzae inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (8) will separate the Lactobacillus casei after purifying is inoculated in the Kolle flask of Lactobacillus casei culture medium, and cultivates 48 hours down at 37 ℃, thereby obtains the primary seed solution of Lactobacillus casei; The Lactobacillus casei culture medium comprise 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, (C.64.H.124.O.26. the molecular formula of Tween 80 is to 1 part Tween 80; English name is POLYSORBATE 80; The Chinese synonym is polyoxyethylene sorbitan monooleate or emulsifying agent T80; Soluble in water, ethanol, methyl alcohol and ethyl acetate; Be mainly used in solubilizer or the emulsifying agent of doing parenteral solution and oral liquid; The dispersant of capsule, the emulsifying agent of ointment and matrix, the matrix of suppository or the emulsifying agent in the food industry) and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2～6.4 scope, thereby guarantees that bacterial strain can have bigger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of Lactobacillus casei is the Lactobacillus casei of the Lactobacillus casei inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (9) will separate the lactobacillus acidophilus after purifying is inoculated in the Kolle flask of lactobacillus acidophilus culture medium, and cultivates 48 hours down at 37 ℃, thereby obtains the primary seed solution of lactobacillus acidophilus; The lactobacillus acidophilus culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2～6.4 scope, thereby guarantees that bacterial strain can have bigger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of lactobacillus acidophilus is the lactobacillus acidophilus of the lactobacillus acidophilus inoculation of medium 100mL of every 500mL.
And/or,
The former bacterial strain seed inclined-plane that (10) will separate the Bifidobacterium after purifying is inoculated in the Kolle flask of Bifidobacterium culture medium, and cultivates 48 hours down at 37 ℃, thereby obtains the primary seed solution of Bifidobacterium; The Bifidobacterium culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water; The pH value of this culture medium should just can be inoculated in 6.2～6.4 scope, thereby guarantees that bacterial strain can have bigger activity, and guarantees that the bacterial strain existing state can not destroyed.Need to prove that the inoculative proportion of Bifidobacterium is the Bifidobacterium of the Bifidobacterium inoculation of medium 100mL of every 500mL.
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Particularly; In practical application; Preferably from the described 10 kinds of poultry of steps A with all selecting the identical weight primary seed solution the primary seed solution of active probiotic, mix then, thereby make the livestock fodder additives that finally makes with these 10 kinds of probios.
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Wherein, Corresponding seed tank culture base comprises 3%～8% brown sugar, 0.2%～0.5% potassium dihydrogen phosphate, 0.3%～0.5% yeast extract, 0.2%～0.5% peptone and the water of surplus, and each constituent content of seed tank culture base described herein is mass percent;
Particularly, in actual production was used, for many-sided factors such as comprehensive cost of manufacture and serviceabilities, each composition of this seed tank culture base accounts for total mass ratio can select the scheme in the following table 1 for use, and the embodiment one in wherein should showing is scheme most preferably:
Table 1:
| Embodiment | Brown sugar | Potassium dihydrogen phosphate | Yeast extract | Peptone | Water |
| Embodiment one | 8％ | 0.22％ | 0.5％ | 0.3％ | Surplus |
| Embodiment two | 7％ | 0.25％ | 0.4％ | 0.3％ | Surplus |
| Embodiment three | 5％ | 0.3％ | 0.3％ | 0.4％ | Surplus |
| Embodiment four | 6％ | 0.2％ | 0.4％ | 0.2％ | Surplus |
Further, many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base accordingly, can may further comprise the steps thereby obtain many bacterial classifications mixing secondary seed solution:
Step C1: preparation seed tank culture base;
Step C2: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to seed tank culture base sterilization 25 minutes;
Particularly, in practical application, preferably select for use under 121 ℃ the seed tank culture base was sterilized 25 minutes, can be killed fully to guarantee the harmful bacterium in the seed tank culture base.
Step C3: many bacterial classifications Hybrid TierI seed liquor is inoculated in the seed tank culture base (many bacterial classifications Hybrid TierI seed liquor that the inoculative proportion according to 3%～5% here is meant 3 to 5 unit volumes of inoculation in the seed tank culture base of per 100 unit volumes) according to 3%～5% inoculative proportion;
Step C4: the seed tank culture base to having inoculated carries out anaerobic fermentation, and the rotating speed of installation for fermenting is 180r/min, and fermentation temperature is 32 ℃, and the pH value in the seed tank culture base reaches fermentation in 4.8 o'clock to be accomplished, and promptly obtains many bacterial classifications and mixes secondary seed solution.Generally speaking, the anaerobic fermentation of seed tank culture base generally can be accomplished in 48～72 hours, and after fermentation was accomplished, the number of active probiotic can reach every milliliter 1,500,000,000 strain.
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out the Chinese medicine fermentation in the Chinese medicine fluid nutrient medium, thereby make the livestock fodder additives that contains the Chinese medicine active probiotic;
Wherein, Described Chinese medicine fluid nutrient medium comprises 5%～8% brown sugar, 2%～3% traditional Chinese medicinal components, 0.2%～0.3% potassium dihydrogen phosphate, 0.3%～0.5% yeast extract and the water of surplus, and each constituent content of Chinese medicine fluid nutrient medium described herein is mass percent; Described traditional Chinese medicinal components is made up of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, gynostemma pentaphylla: 60～80 parts, wilsonii: 60～80 parts,
The root of large-flowered skullcap: 50～80 parts, Radix Isatidis: 40～60 parts, the Chinese bulbul: 40～60 parts, honeysuckle: 40～60 parts,
Radix Angelicae Sinensis: 40～60 parts, hawthorn: 50～80 parts, dried orange peel: 50～80 parts, purslane: 40～60 parts,
Radix Glycyrrhizae: 30～50 parts.
Particularly; In actual production is used; For many-sided factors such as comprehensive cost of manufacture and serviceabilities, each composition of this Chinese medicine fluid nutrient medium accounts for total mass ratio can select the preferred version in the following table 2 for use, and the embodiment 1 in wherein should showing is a most preferred embodiment:
Table 2:
| Embodiment | Brown sugar | Traditional Chinese medicinal components | Potassium dihydrogen phosphate | Yeast extract | Water |
| Embodiment 1 | 6％ | 3％ | 0.2％ | 0.5％ | Surplus |
| Embodiment 2 | 5％ | 2％ | 0.25％ | 0.3％ | Surplus |
| Embodiment 3 | 7％ | 2.5％ | 0.25％ | 0.35％ | Surplus |
| Embodiment 4 | 8％ | 2.5％ | 0.3％ | 0.4％ | Surplus |
Corresponding with it, the composition of traditional Chinese medicinal components, and each composition accordingly accounts for the parts by weight of traditional Chinese medicinal components total amount can select the preferred version in the following table 3 for use, and wherein the unit in this table is weight portion, and embodiment a is a most preferred embodiment:
Table 3:
| The traditional Chinese medicine ingredients title | Instance a | Instance b | Instance c | Instance d |
| The Radix Astragali | 80 | 60 | 70 | 75 |
| Radix Codonopsis | 80 | 75 | 70 | 60 |
| Gynostemma pentaphylla | 80 | 60 | 70 | 75 |
| Wilsonii | 80 | 75 | 70 | 60 |
| The root of large-flowered skullcap | 50 | 60 | 70 | 80 |
| Radix Isatidis | 50 | 60 | 55 | 40 |
| The Chinese bulbul | 50 | 40 | 55 | 60 |
| Honeysuckle | 50 | 60 | 55 | 40 |
| Radix Angelicae Sinensis | 60 | 40 | 50 | 55 |
| Hawthorn | 50 | 80 | 70 | 60 |
| Dried orange peel | 60 | 50 | 60 | 70 |
| Purslane | 50 | 60 | 55 | 40 |
| Radix Glycyrrhizae | 50 | 30 | 40 | 45 |
Further, many bacterial classifications mixing secondary seed solution are inoculated into carry out the Chinese medicine fermentation in the Chinese medicine fluid nutrient medium accordingly, can may further comprise the steps thereby make the livestock fodder additives that contains the Chinese medicine active probiotic:
Step D1: preparation Chinese medicine fluid nutrient medium;
Step D2: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to herb liquid body medium sterilization 20 minutes;
Particularly, in practical application, preferably select for use under 121 ℃, can be killed fully to guarantee the harmful bacterium in the Chinese medicine fluid nutrient medium to herb liquid body medium sterilization 20 minutes.
Step D3: many bacterial classifications are mixed secondary seed solution be inoculated into (the inoculative proportion according to 5%～10% here is meant that many bacterial classifications of 5 to 10 unit volumes of Chinese medicine liquid inoculation of medium of per 100 unit volumes mix secondary seed solution) in the Chinese medicine fluid nutrient medium according to 5%～10% inoculative proportion;
Step D4: the Chinese medicine fluid nutrient medium to having inoculated carries out anaerobic fermentation; The rotating speed of installation for fermenting is 180r/min; Fermentation temperature is 32 ℃, and the pH value in the Chinese medicine fluid nutrient medium reaches fermentation in 5.0 o'clock to be accomplished, and promptly obtains to contain the livestock fodder additives of Chinese medicine active probiotic; Generally speaking, the anaerobic fermentation of Chinese medicine fluid nutrient medium generally can be accomplished in 48～72 hours, and after fermentation was accomplished, the number of active probiotic can reach every milliliter 2,000,000,000 strain.
In technique scheme, the effect of each composition of traditional Chinese medicinal components of the present invention is: the Radix Astragali---sweet warm in nature, the tonifying Qi and lifting yang of distinguishing the flavor of, and its active component astragalus polyose can improve immunity of organisms; Radix Codonopsis---the sweet property of distinguishing the flavor of is flat, tonifying middle-Jiao and Qi and taste, and its active component saponin can improve immunity of organisms; Gynostemma pentaphylla---toxin expelling, regulate immunologic function, the total saponin of its active component is three times of genseng; Wilsonii---flavor is hot warm in nature, anti-stress regulate the body disorder, but its active component eleutheroside irritation cell immunity: the root of large-flowered skullcap---rush down excess fire, except that damp and hot, its active component baicalin anti-inflammatory, antibiotic; Radix Isatidis---have clearing heat and detoxicating, cool blood anti-inflammatory, its active component is indigo, indigo red etc. has resisting pathogenic microbes, raise immunity; The Chinese bulbul---heat-clearing and damp-drying drug, removing pattogenic heat from the blood and toxic material from the body; Honeysuckle---clearing heat and detoxicating, antibacterial anti-inflammatory, antiviral, adjusting immunity of organism; Radix Angelicae Sinensis---it is sweet warm in nature to distinguish the flavor of, and can enrich blood, and can invigorate blood circulation again, and its active component sodium ferulate ability activating macrophage plays immunologic function; Hawthorn---promoting digestion and removing indigestion, the promoting the circulation of qi stasis of blood of loosing, antidiarrheal dysentery; Dried orange peel---promoting the circulation of qi invigorating the spleen is whetted the appetite in the accent, and its active component volatile oil, hesperidine have the function that promotes digestive juice secretion, improve a poor appetite; Purslane---clearing heat and detoxicating, strengthening spleen and nourishing stomach, expelling parasite; Radix Glycyrrhizae---have clearing heat and detoxicating, coordinating the drug actions of a prescription, its active component glycyrrhizic acid is a cell immunomodulator.The mentioned traditional Chinese medicinal components of the present invention is according to growth characteristic of poultry and the breed demand in the poultry farming process; The drug matching principle of adopt monarch, minister, helping, make; It is formulated to choose a few flavor Chinese medicines that can promote poultry digestion and growth, can strengthen the poultry immunity of organisms, can improve the poultry resistance against diseases respectively---and at first select for use the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii as monarch drug in a prescription, improve animal body immunity: select for use the root of large-flowered skullcap, the Chinese bulbul, Radix Isatidis, honeysuckle to improve the poultry resistance against diseases simultaneously; And select for use Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane to promote digestion and growth; Select for use the Radix Glycyrrhizae not only can be clearing heat and detoxicating and can also be in harmonious proportion all the other all medicines at last.This shows that traditional Chinese medicinal components provided by the present invention not only can promote the speed of growth of poultry, and can strengthen the disease resistance ability of poultry, even can also treat some bird common diseases.
Need to prove that strain culturing equipment used in the present invention and the strain culturing technology of not describing in detail all can adopt strain culturing equipment general in the prior art and strain culturing technology, do not remake detailed description in this application.Various Chinese medicines described in the present invention all can be buied in each big Chinese medicine pharmacy easily, therefore this are no longer detailed.The former bacterial strain of various active probiotic bacterium described in the present invention all can be introduced from Chinese common micro-organisms culture presevation administrative center (CGMCC) or agriculture microorganism fungus kind preservation administrative center (ACCC), with existing state and the survival quality of guaranteeing each bacterial classification.
It is thus clear that; The enforcement of the embodiment of the invention can significantly improve the active and field planting performance in enteron aisle of bacterial strain of active probiotic in the livestock fodder additives; And make the livestock fodder additives that makes be rich in traditional Chinese medicine ingredients; The active component that plays pharmacological action in the traditional Chinese medicine ingredients is given full play to, performance and growth promotion performance thereby the strong body of raising livestock fodder additives is cured the disease, and then replace antibiotic use fully.
The above; Be merely the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technical staff who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of claims.
Claims (4)
1. a manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic is characterized in that, comprising:
Steps A: choose the multiple poultry that equates bacterial strain quantity former bacterial strain, and respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity expansion cultivation with the former bacterial strain of active probiotic, thereby obtain the primary seed solution of every kind of poultry with active probiotic with active probiotic;
Wherein, described multiple poultry with active probiotic comprise bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast 、 Chan ?in Candida, aspergillus niger, Rhizopus oryzae, Lactobacillus casei, lactobacillus acidophilus and the Bifidobacterium at least two kinds;
Step B: every kind of poultry of equal weight is mixed with the primary seed solution of active probiotic, thereby obtain many bacterial classifications Hybrid TierI seed liquor;
Step C: many bacterial classifications Hybrid TierI seed liquor is inoculated into carries out many bacterial classifications Mixed culture in the seed tank culture base, mix secondary seed solution thereby obtain many bacterial classifications;
Step D: many bacterial classifications are mixed secondary seed solution be inoculated into and carry out the Chinese medicine fermentation in the Chinese medicine fluid nutrient medium, thereby make the livestock fodder additives that contains the Chinese medicine active probiotic;
Wherein, described Chinese medicine fluid nutrient medium comprises 5%～8% brown sugar, 2%～3% traditional Chinese medicinal components, 0.2%～0.3% potassium dihydrogen phosphate, 0.3%～0.5% yeast extract and the water of surplus; Described traditional Chinese medicinal components is made up of the Radix Astragali, Radix Codonopsis, gynostemma pentaphylla, wilsonii, the root of large-flowered skullcap, Radix Isatidis, the Chinese bulbul, honeysuckle, Radix Angelicae Sinensis, hawthorn, dried orange peel, purslane and Radix Glycyrrhizae, and the parts by weight that its each composition accounts for the traditional Chinese medicinal components total amount are:
The Radix Astragali: 60～80 parts, Radix Codonopsis: 60～80 parts, gynostemma pentaphylla: 60～80 parts, wilsonii: 60～80 parts,
The root of large-flowered skullcap: 50～80 parts, Radix Isatidis: 40～60 parts, the Chinese bulbul: 40～60 parts, honeysuckle: 40～60 parts,
Radix Angelicae Sinensis: 40～60 parts, hawthorn: 50～80 parts, dried orange peel: 50～80 parts, purslane: 40～60 parts,
Radix Glycyrrhizae: 30～50 parts.
2. the manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic according to claim 1; It is characterized in that; The described former bacterial strain of choosing the multiple poultry of equal bacterial strain quantity with active probiotic; And respectively every kind of poultry is carried out single bacterial classification bacterial strain quantity with the former bacterial strain of active probiotic and expand and cultivate, comprise with the primary seed solution of active probiotic thereby obtain every kind of poultry:
The former bacterial strain seed inclined-plane of bacillus subtilis after separate purifying is inoculated in the triangular flask of bacillus subtilis bacterium culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bacillus subtilis; The bacillus subtilis bacterium culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane of the bafillus natto after separate purifying is inoculated in the triangular flask of bafillus natto culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bafillus natto; The bafillus natto culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane of bacillus licheniformis after separate purifying is inoculated in the triangular flask of bacillus licheniformis culture medium, and shaken cultivation 24 hours, thereby obtains the primary seed solution of bacillus licheniformis; The bacillus licheniformis culture medium comprises 3 parts beef extract, 10 parts peptone, 5 parts sodium chloride, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane of the brewer's yeast after separate purifying is inoculated in the triangular flask of brewer's yeast culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of brewer's yeast; The brewer's yeast culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
With De Chan after separate purifying ?the former bacterial strain seed inclined-plane of Candida be inoculated into Chan ?in the triangular flask of Candida culture medium, and shaken cultivation 48 hours, thus Huo De Chan ?the primary seed solution of Candida; Chan ?the Candida culture medium comprise 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane of the aspergillus niger after separate purifying is inoculated in the triangular flask of aspergillus niger culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of aspergillus niger; The aspergillus niger culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane of the Rhizopus oryzae after separate purifying is inoculated in the triangular flask of Rhizopus oryzae culture medium, and shaken cultivation 48 hours, thereby obtains the primary seed solution of Rhizopus oryzae; The Rhizopus oryzae culture medium comprises 200 parts potato, 20 parts sucrose, 3 parts potassium dihydrogen phosphate, 1.5 parts magnesium sulfate, 20 parts agar and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane that separates the Lactobacillus casei after purifying is inoculated in the Kolle flask of Lactobacillus casei culture medium, and cultivated 48 hours down at 37 ℃, thus the primary seed solution of acquisition Lactobacillus casei; The Lactobacillus casei culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane that separates the lactobacillus acidophilus after purifying is inoculated in the Kolle flask of lactobacillus acidophilus culture medium, and cultivated 48 hours down at 37 ℃, thus the primary seed solution of acquisition lactobacillus acidophilus; The lactobacillus acidophilus culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water;
And/or,
The former bacterial strain seed inclined-plane that separates the Bifidobacterium after purifying is inoculated in the Kolle flask of Bifidobacterium culture medium, and cultivated 48 hours down at 37 ℃, thus the primary seed solution of acquisition Bifidobacterium; The Bifidobacterium culture medium comprises 20 parts glucose, 10 parts peptone, 10 parts beef extract, 5 parts yeast extract, 5 parts natrium citricum, 2 parts sodium dihydrogen phosphate, 5 parts sodium acetate, 0.2 part epsom salt, 0.25 part four water manganese sulfates, 1 part Tween 80 and 1000 parts water.
3. according to claim 1 and the 2 described manufacturing approaches that contain the livestock fodder additives of Chinese medicine active probiotic; It is characterized in that; Described many bacterial classifications Hybrid TierI seed liquor is inoculated into carried out many bacterial classifications Mixed culture in the seed tank culture base, may further comprise the steps thereby obtain many bacterial classifications mixing secondary seed solution:
Step C1: preparation seed tank culture base;
Wherein, described seed tank culture base comprises 3%～8% brown sugar, 0.2%～0.5% potassium dihydrogen phosphate, 0.3%～0.5% yeast extract, 0.2%～0.5% peptone and the water of surplus;
Step C2: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to seed tank culture base sterilization 25 minutes;
Step C3: many bacterial classifications Hybrid TierI seed liquor is inoculated in the seed tank culture base according to 3%～5% inoculative proportion;
Step C4: the seed tank culture base to having inoculated carries out anaerobic fermentation, and the rotating speed of installation for fermenting is 180r/min, and fermentation temperature is 32 ℃, and the pH value in the seed tank culture base reaches fermentation in 4.8 o'clock to be accomplished, and promptly obtains many bacterial classifications and mixes secondary seed solution.
4. the manufacturing approach that contains the livestock fodder additives of Chinese medicine active probiotic according to claim 1 and 2; It is characterized in that; Described many bacterial classifications mixing secondary seed solution are inoculated into carried out the Chinese medicine fermentation in the Chinese medicine fluid nutrient medium, may further comprise the steps thereby make the livestock fodder additives that contains the Chinese medicine active probiotic:
Step D1: preparation Chinese medicine fluid nutrient medium;
Step D2: under 115 ℃～121 ℃,, be cooled to 30 ℃ again to herb liquid body medium sterilization 20 minutes;
Step D3: many bacterial classifications are mixed secondary seed solution be inoculated in the Chinese medicine fluid nutrient medium according to 5%～10% inoculative proportion;
Step D4: the Chinese medicine fluid nutrient medium to having inoculated carries out anaerobic fermentation; The rotating speed of installation for fermenting is 180r/min; Fermentation temperature is 32 ℃, and the pH value in the Chinese medicine fluid nutrient medium reaches fermentation in 5.0 o'clock to be accomplished, and promptly obtains to contain the livestock fodder additives of Chinese medicine active probiotic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102764143A CN102366021B (en) | 2011-09-16 | 2011-09-16 | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102764143A CN102366021B (en) | 2011-09-16 | 2011-09-16 | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102366021A true CN102366021A (en) | 2012-03-07 |
| CN102366021B CN102366021B (en) | 2013-01-23 |
Family
ID=45758641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011102764143A Expired - Fee Related CN102366021B (en) | 2011-09-16 | 2011-09-16 | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102366021B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696870A (en) * | 2012-06-14 | 2012-10-03 | 天津吉亚牧业集团有限公司 | Natto biological feed for livestock and poultry and production method thereof |
| CN102894185A (en) * | 2012-11-12 | 2013-01-30 | 哈尔滨市华达饲料制造有限公司 | Fermented radix isatidis feed additive and application thereof |
| CN103039700A (en) * | 2013-01-09 | 2013-04-17 | 广州华农大实验兽药有限公司 | Feed additive for veterinary drug solid fermentation and preparation method of feed additive |
| CN103349161A (en) * | 2013-07-29 | 2013-10-16 | 雨润慕德生物科技（连云港）有限公司 | Acanthopanax senticosus contained livestock and poultry microbial additive and preparation method thereof |
| CN103892138A (en) * | 2014-04-21 | 2014-07-02 | 吉林农业大学 | Synbiotics for enhancing health of breast-feed calf and preparation method thereof |
| CN104509748A (en) * | 2014-12-25 | 2015-04-15 | 广西桂多宝生物技术有限公司 | Chicken feed additive and preparation method thereof |
| CN104543375A (en) * | 2015-02-02 | 2015-04-29 | 广东迈科特生物科技有限公司 | Compound fermented Chinese herbal medicine preparation for livestock and poultry and preparation method and applications thereof |
| CN104543596A (en) * | 2015-02-02 | 2015-04-29 | 广东迈科特生物科技有限公司 | Special composite vitamin premix feed preparation for breeding poultry as well as preparation method and application thereof |
| CN104543372A (en) * | 2015-01-18 | 2015-04-29 | 新疆瑞源丰贸易有限公司 | Sunflower disk composite feed and preparation method thereof |
| CN104982674A (en) * | 2015-04-30 | 2015-10-21 | 新昌县大成生物科技有限公司 | Preparation method of probiotic fermentation duck feed additive |
| CN104982673A (en) * | 2015-04-30 | 2015-10-21 | 新昌县大成生物科技有限公司 | Preparation method of probiotic fermentation chicken feed additive |
| CN106074812A (en) * | 2016-08-29 | 2016-11-09 | 兴文县鸿程农业生态科技开发有限责任公司 | A kind of Chinese medicine compound for preventing and treating bacterial disease of fowl and application |
| CN106344761A (en) * | 2016-08-30 | 2017-01-25 | 福建贝迪药业有限公司 | Fermentation Chinese herbal medicine for improving livestock and poultry immunity and preparing method thereof |
| CN108740306A (en) * | 2018-05-03 | 2018-11-06 | 武汉环绿时代生态园林工程有限公司 | A kind of feed addictive and its feeding method |
| CN109456907A (en) * | 2019-01-08 | 2019-03-12 | 田洪涛 | A kind of Chinese medicine living bacterial liquid and preparation method thereof |
| CN110279764A (en) * | 2019-07-16 | 2019-09-27 | 广西富凤农牧集团有限公司 | A kind of fermented tcm additive and preparation method thereof improving poultry intestinal tract health |
| CN114847399A (en) * | 2022-03-17 | 2022-08-05 | 魏广军 | Silage additive solution containing traditional Chinese medicines and probiotics and preparation method thereof |
| CN115678789A (en) * | 2022-11-04 | 2023-02-03 | 安徽农业大学 | Novel method for improving activity of beer yeast and preparation of composite microbial inoculum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1043428A (en) * | 1989-10-13 | 1990-07-04 | 蒋祥太 | Additives poultry composite feed and preparation method thereof |
| CN1831107A (en) * | 2006-03-15 | 2006-09-13 | 南京仕必得生物技术有限公司 | Traditional Chinese medicine solid type mixed culture fermentation tech. and its application |
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
-
2011
- 2011-09-16 CN CN2011102764143A patent/CN102366021B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1043428A (en) * | 1989-10-13 | 1990-07-04 | 蒋祥太 | Additives poultry composite feed and preparation method thereof |
| CN1831107A (en) * | 2006-03-15 | 2006-09-13 | 南京仕必得生物技术有限公司 | Traditional Chinese medicine solid type mixed culture fermentation tech. and its application |
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《安徽农业科学》 20091231 杨帆 "中草药饲料添加剂在畜禽生产中的应用" 24-26 , 第12期 * |
| 杨帆: ""中草药饲料添加剂在畜禽生产中的应用"", 《安徽农业科学》 * |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696870A (en) * | 2012-06-14 | 2012-10-03 | 天津吉亚牧业集团有限公司 | Natto biological feed for livestock and poultry and production method thereof |
| CN102894185A (en) * | 2012-11-12 | 2013-01-30 | 哈尔滨市华达饲料制造有限公司 | Fermented radix isatidis feed additive and application thereof |
| CN102894185B (en) * | 2012-11-12 | 2013-10-30 | 哈尔滨市华达饲料制造有限公司 | Fermented radix isatidis feed additive and application thereof |
| CN103039700A (en) * | 2013-01-09 | 2013-04-17 | 广州华农大实验兽药有限公司 | Feed additive for veterinary drug solid fermentation and preparation method of feed additive |
| CN103039700B (en) * | 2013-01-09 | 2014-06-04 | 广州华农大实验兽药有限公司 | Feed additive for veterinary drug solid fermentation and preparation method of feed additive |
| CN103349161A (en) * | 2013-07-29 | 2013-10-16 | 雨润慕德生物科技（连云港）有限公司 | Acanthopanax senticosus contained livestock and poultry microbial additive and preparation method thereof |
| CN103892138A (en) * | 2014-04-21 | 2014-07-02 | 吉林农业大学 | Synbiotics for enhancing health of breast-feed calf and preparation method thereof |
| CN104509748B (en) * | 2014-12-25 | 2018-08-10 | 东阳市新意工业产品设计有限公司 | A kind of chicken feed addictive and preparation method thereof |
| CN104509748A (en) * | 2014-12-25 | 2015-04-15 | 广西桂多宝生物技术有限公司 | Chicken feed additive and preparation method thereof |
| CN104543372A (en) * | 2015-01-18 | 2015-04-29 | 新疆瑞源丰贸易有限公司 | Sunflower disk composite feed and preparation method thereof |
| CN104543596A (en) * | 2015-02-02 | 2015-04-29 | 广东迈科特生物科技有限公司 | Special composite vitamin premix feed preparation for breeding poultry as well as preparation method and application thereof |
| CN104543375A (en) * | 2015-02-02 | 2015-04-29 | 广东迈科特生物科技有限公司 | Compound fermented Chinese herbal medicine preparation for livestock and poultry and preparation method and applications thereof |
| CN104982674A (en) * | 2015-04-30 | 2015-10-21 | 新昌县大成生物科技有限公司 | Preparation method of probiotic fermentation duck feed additive |
| CN104982673A (en) * | 2015-04-30 | 2015-10-21 | 新昌县大成生物科技有限公司 | Preparation method of probiotic fermentation chicken feed additive |
| CN106074812B (en) * | 2016-08-29 | 2020-01-21 | 兴文县鸿程农业生态科技开发有限责任公司 | Traditional Chinese medicine compound for preventing and treating poultry bacterial diseases and application |
| CN106074812A (en) * | 2016-08-29 | 2016-11-09 | 兴文县鸿程农业生态科技开发有限责任公司 | A kind of Chinese medicine compound for preventing and treating bacterial disease of fowl and application |
| CN106344761A (en) * | 2016-08-30 | 2017-01-25 | 福建贝迪药业有限公司 | Fermentation Chinese herbal medicine for improving livestock and poultry immunity and preparing method thereof |
| CN106344761B (en) * | 2016-08-30 | 2019-07-05 | 福建贝迪药业有限公司 | A kind of fermented type Chinese herbal and crude drugs preparations and preparation method thereof improving immunity of livestock |
| CN108740306A (en) * | 2018-05-03 | 2018-11-06 | 武汉环绿时代生态园林工程有限公司 | A kind of feed addictive and its feeding method |
| CN109456907A (en) * | 2019-01-08 | 2019-03-12 | 田洪涛 | A kind of Chinese medicine living bacterial liquid and preparation method thereof |
| CN110279764A (en) * | 2019-07-16 | 2019-09-27 | 广西富凤农牧集团有限公司 | A kind of fermented tcm additive and preparation method thereof improving poultry intestinal tract health |
| CN114847399A (en) * | 2022-03-17 | 2022-08-05 | 魏广军 | Silage additive solution containing traditional Chinese medicines and probiotics and preparation method thereof |
| CN115678789A (en) * | 2022-11-04 | 2023-02-03 | 安徽农业大学 | Novel method for improving activity of beer yeast and preparation of composite microbial inoculum |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102366021B (en) | 2013-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102366021B (en) | Manufacturing method of poultry feed additive containing traditional Chinese medicinal activity probiotics | |
| CN102366024B (en) | Production method of pig feed additive containing traditional Chinese medicine and active probiotics | |
| CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN102366025B (en) | Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics | |
| CN102894207B (en) | Body-immunity-enhancing composite Chinese herbal medicine pig feed additive and preparation method thereof | |
| CN102367415A (en) | Traditional Chinese medicine probiotics liquid culture medium for pig feed, culture method and feed additive | |
| CN106754619A (en) | It is a kind of that the method that traditional Chinese medicinal components promote growth of probiotics is added in grain culture medium | |
| CN102367418B (en) | Traditional Chinese medicine probiotics liquid medium used for pig feed, culturing method and feed additive | |
| CN104522358A (en) | Chinese herbal medicine microbial ecological agent for improving weight of weaned pig and preparation method of Chinese herbal medicine microbial ecological agent | |
| CN102367416B (en) | Traditional Chinese medicine probiotic solid culture medium for cow feed, culture method and feed additive | |
| CN102206590B (en) | Preparation methods of Mycobacterium plei Sq-1 and composite microecological preparation thereof | |
| CN103636946A (en) | Preparation method and application of Chinese herbal medicine immunological enhancer for finless eel | |
| CN105360665A (en) | Anti-diarrhea Chinese herbal medicine feed additive for weaned piglets and preparation method thereof | |
| CN105104863A (en) | Pig feed for easily promoting pig growth | |
| CN106074941A (en) | A kind of preparation method of the Chinese herbal medicinal active fermentation broth preventing foot and mouth disease | |
| CN102367419B (en) | Traditional Chinese medicine probiotics liquid culture medium for poultry feed, culture method and feed additive | |
| CN102367420B (en) | Traditional Chinese medicine probiotic liquid culture medium used in cow forage, culturing method, and forage additive | |
| CN102367417B (en) | Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive | |
| CN109363004A (en) | The preparation method and application of big squama Barb fish fermented type Chinese medicine immunity enhancer | |
| CN107961260A (en) | Treatment, the medicament for preventing birds coliform diarrhea disease and preparation method thereof | |
| CN103082361A (en) | Method for preparing purple sweet potato drink from compound probiotics | |
| CN106215011A (en) | A kind of preparation method of the Chinese herbal medicinal active fermentation broth preventing swine fever | |
| CN109965110A (en) | A kind of feed addictive preventing cow subclinical mastitis | |
| CN115216426B (en) | Bacillus amyloliquefaciens LL-2 and application thereof | |
| CN107233477A (en) | A kind of composition, preparation method and application method for being used to treat porcine respiratory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190816 Address after: 116000 No. 229-4 Zhongshan District, Dalian City, Liaoning Province Patentee after: Hualin (Liaoning) Biotechnology Co.,Ltd. Address before: 116201 No. 182 Nansha Street, Shahekou District, Dalian City, Liaoning Province, 6-1 Patentee before: Wang Songlin |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130123 |