CN114041536A - Composite Chinese herbal medicine microecological preparation and preparation method thereof - Google Patents
Composite Chinese herbal medicine microecological preparation and preparation method thereof Download PDFInfo
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- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
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Abstract
The invention belongs to the technical field of cow and sheep microecological preparations, and discloses a compound Chinese herbal medicine microecological preparation and a preparation method thereof, wherein the compound Chinese herbal medicine microecological preparation comprises 20-40 parts of radix isatidis extract, 15-30 parts of licorice extract, 15-20 parts of astragalus extract, 10-15 parts of poria cocos, 8-15 parts of chrysanthemum, 6-12 parts of fructus forsythiae, 5-10 parts of honeysuckle, 10-20 parts of yeast cell wall polysaccharide, 3-5 parts of a compound microbial inoculum, 0.2-0.3 part of salt and the balance of sterilized deionized water. According to the invention, the composite microbial inoculum, the zymophyte polysaccharide and multiple Chinese herbal medicines are added into the composition, and the components have interaction, so that gastrointestinal peristalsis is promoted, the intestinal flora environment of ruminants such as cattle and sheep is improved, and the interaction of the components in the microecological preparation can ensure that a sufficient number of viable bacteria can smoothly reach the intestinal part and be used for improving the intestinal environment of the ruminants such as cattle and sheep, so that the stability of the microecological preparation is improved, and the effective period of the microecological preparation is prolonged.
Description
Technical Field
The invention belongs to the technical field of cattle and sheep microecological preparations, and particularly relates to a compound Chinese herbal medicine microecological preparation and a preparation method thereof.
Background
At present, China is a cattle and sheep breeding nation, breeding modes are developing towards standardization, intensification and scale at present, in order to maximize economic benefits, the proportion of concentrated feed is gradually increased in cattle and sheep breeding and fattening, and the proportion of coarse feed is gradually decreased, so that the aim of rapid marketing is fulfilled. The increase of concentrated feed causes the intestinal tract digestive system of ruminants such as cattle, sheep and the like to be worse and worse, the gas discharged is more and more, and the microecological bacterial colony in the intestines and the stomach is unbalanced, so that various digestive system diseases appear, and the health of the ruminants is influenced.
Through the above analysis, the problems and defects of the prior art are as follows: in the prior art, no Chinese herbal medicine microecological preparation for regulating the intestinal tract of ruminants such as cattle and sheep is available.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a compound Chinese herbal medicine microecological preparation and a preparation method thereof.
The invention is realized in such a way that a preparation method of a composite Chinese herbal medicine microecological preparation comprises the following steps:
step one, obtaining yeast, cleaning the obtained yeast by using deionized water, freezing the cleaned yeast for 3 hours at the temperature of 2-3 ℃, thawing the yeast in water bath at the temperature of 50 ℃, centrifuging the thawed yeast, and repeating for 2 times to obtain a precipitate; performing enzymolysis autolysis on the precipitate, centrifuging, collecting supernatant, concentrating the collected supernatant, precipitating with ethanol, and vacuum drying to constant weight to obtain yeast cell wall polysaccharide;
step two, preparing the composite microbial inoculum powder: boiling the sterilized deionized water, sequentially adding sucrose, tea leaves and potassium dihydrogen phosphate, boiling the water again, and filtering out the tea leaves by using gauze; naturally cooling to 30 ℃, subpackaging in a wide-mouth glass bottle, adding a mother solution of a composite microbial inoculum prepared by mixing lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus according to a ratio of 1:2:2, sealing kraft paper, and standing and culturing at 30 ℃ for 15 days to serve as a strain mother solution; mixing the cassava powder or the corn powder with wheat bran, spraying strain mother liquor with the weight of 50-100%, and drying to obtain composite microbial inoculum powder for later use;
step three, placing the isatis root in sterilized deionized water for decocting for three times; after the decoction is finished, putting the decoction on inorganic ceramic membrane filtering equipment or organic tubular ultrafiltration membrane filtering equipment for filtering at normal temperature, and combining the filtrates to obtain a crude extract; centrifuging the crude extractive solution in a three-leg centrifuge, and performing nanofiltration on nanofiltration membrane filtration equipment to obtain retentate which is radix Isatidis extract;
step four, mixing the glycyrrhiza glabra with 85-90% of ethanol according to the weight ratio of 1: 10-12, mixing and then stirring at high speed; placing the crushed glycyrrhiza glabra in ultrasonic waves for ultrasonic treatment, and performing vacuum filtration to obtain filtrate; recovering ethanol from the filtrate under reduced pressure, concentrating, and spray drying to obtain Glycyrrhrizae radix extract;
step five, crushing the astragalus, and sieving the crushed astragalus through a sieve of 30-40 meshes to obtain coarse astragalus powder; adding 0.5-2 times of edible alcohol and an extracting agent into the astragalus coarse powder in an extraction kettle for infiltration and extraction, and then putting the extract into a separation kettle for evaporation and recovery of the extracting agent to obtain astragalus coarse extract; concentrating the radix astragali crude extract under reduced pressure until the ethanol content in the concentrated solution is less than 5%, and spray drying to obtain radix astragali extract;
step six, weighing the isatis root extract, the liquorice extract, the astragalus extract, the poria cocos, the chrysanthemum, the fructus forsythiae, the honeysuckle, the yeast cell wall polysaccharide, the composite microbial inoculum, the salt and the sterilized deionized water in sequence according to the mass parts for later use;
and seventhly, crushing the poria cocos, the chrysanthemum, the fructus forsythiae and the honeysuckle, sequentially adding the radix isatidis extract, the liquorice extract, the astragalus extract, the yeast cell wall polysaccharide and the salt, inoculating 25-50% of composite microbial agent powder, adding sterilized deionized water, stirring uniformly, and standing and culturing to obtain the composite Chinese herbal medicine microecological preparation.
Further, in step one, the centrifugation comprises: centrifuging for 15min at the speed of 1000-2000r/min by using a centrifugal device.
Further, in the third step, 8 times of sterilized deionized water by weight is added in the first decoction, and the decoction time is 80-120 min; adding 6 times of sterilized deionized water for the second decoction, wherein the decoction time is 40-60 min; and adding 3 times of sterilized deionized water by weight into the third decoction, wherein the decoction time is 15-30 min.
Further, in the third step, the centrifugation method is as follows: and (3) placing the crude extract on a three-leg centrifuge with the rotating speed of 6000-8000 turns for centrifuging for 20-35 min.
Further, in the fifth step, the rotating speed during high-speed stirring is 3000-6000 rmp, and the working frequency during ultrasonic treatment is 500-1000 Hz.
Further, in the fifth step, the pressure of the reduced pressure concentration is 0.06-0.12 Mpa, the temperature is 55-85 ℃, and the time of the reduced pressure concentration is 10-20 min.
Further, in the seventh step, the conditions of the static culture are as follows: and (3) placing the mixture in an environment of 25-35 ℃ for static culture for 7-14 days to obtain the compound Chinese herbal medicine microecological preparation.
The invention also aims to provide a composite Chinese herbal medicine microecological preparation prepared by the preparation method of the composite Chinese herbal medicine microecological preparation, wherein the composite Chinese herbal medicine microecological preparation comprises, by mass, 20-40 parts of isatis root extract, 15-30 parts of licorice extract, 15-20 parts of astragalus extract, 10-15 parts of poria cocos, 8-15 parts of chrysanthemum, 6-12 parts of fructus forsythiae, 5-10 parts of honeysuckle, 10-20 parts of yeast cell wall polysaccharide, 3-5 parts of composite microbial agent, 0.2-0.3 part of salt and the balance of sterilized deionized water.
Further, the compound microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus according to the proportion of 1:2: 2.
The invention also aims to provide application of the compound Chinese herbal medicine microecological preparation in preparation of feed and feed additives for ruminants such as cattle, sheep and the like.
By combining all the technical schemes, the invention has the advantages and positive effects that: according to the invention, the weight ratio of lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus is controlled, so that the number of bacteria in intestinal tracts is balanced, the growth of harmful bacteria and pathogenic bacteria is effectively inhibited, the beneficial flora in the intestinal tracts is effectively balanced, the yeast wall polysaccharide and various Chinese herbal medicines are added into the composition, interaction is exerted among the components, gastrointestinal tract peristalsis is promoted together, intestinal tract organism cells are activated, the toxin retained in the intestinal tracts can be timely and efficiently discharged out of the body, the intestinal flora environment of ruminants such as cattle and sheep is further improved, the interaction of various components in the microecological preparation can ensure that a sufficient number of viable bacteria can smoothly reach the intestinal tract part and be used for improving the intestinal environment of the ruminants such as cattle and sheep, the stability of the microecological preparation is improved, and the effective period of the microecological preparation is further prolonged.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
Fig. 1 is a flow chart of a preparation method of the compound Chinese herbal medicine microecological preparation provided by the embodiment of the invention.
Fig. 2 is a flow chart of a preparation method of the isatis root extract provided by the embodiment of the invention.
FIG. 3 is a flow chart of a method for preparing a licorice extract according to an embodiment of the present invention.
FIG. 4 is a flow chart of the method for preparing the Astragalus membranaceus extract according to the embodiment of the present invention.
Fig. 5 is a flowchart of a method for preparing complex microbial inoculum bacterial powder provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a compound Chinese herbal medicine microecological preparation and a preparation method thereof, and the invention is described in detail with reference to the accompanying drawings.
The composite Chinese herbal medicine microecological preparation provided by the embodiment of the invention comprises, by mass, 20-40 parts of an isatis root extract, 15-30 parts of a licorice extract, 15-20 parts of an astragalus extract, 10-15 parts of poria cocos, 8-15 parts of chrysanthemum, 6-12 parts of fructus forsythiae, 5-10 parts of honeysuckle, 10-20 parts of yeast cell wall polysaccharide, 3-5 parts of a composite microbial agent, 0.2-0.3 part of salt and the balance of sterilized deionized water.
The composite microbial inoculum provided by the embodiment of the invention is prepared from acetic acid bacteria and yeast according to the mass ratio of 1: 1 are mixed.
As shown in fig. 1, the preparation method of the compound Chinese herbal medicine microecological preparation provided by the embodiment of the invention comprises the following steps:
s101, preparing yeast cell wall polysaccharide, an isatis root extract, a liquorice root extract, an astragalus root extract and composite microbial agent powder for later use respectively;
s102, weighing radix isatidis extract, liquorice extract, astragalus extract, poria cocos, chrysanthemum, fructus forsythiae, honeysuckle, yeast cell wall polysaccharide, composite microbial inoculum, salt and sterilized deionized water in sequence according to parts by mass for later use;
s103, crushing poria cocos, chrysanthemum, fructus forsythiae and honeysuckle, sequentially adding an isatis root extract, a licorice root extract, an astragalus root extract, yeast cell wall polysaccharide and salt, inoculating 25-50% of composite microbial agent powder, adding sterilized deionized water, uniformly stirring, and standing and culturing to obtain the composite Chinese herbal medicine microecological preparation.
The preparation method of the yeast cell wall polysaccharide provided by the embodiment of the invention comprises the following steps:
obtaining yeast, cleaning the obtained yeast by using deionized water, freezing the cleaned yeast for 3h at the temperature of 2-3 ℃, thawing the yeast in water bath at the temperature of 50 ℃, centrifuging the thawed yeast for 15min at the speed of 1000-2000r/min by using a centrifugal device, and repeating the centrifugation for 2 times to obtain a precipitate; and performing enzymolysis autolysis on the precipitate, centrifuging, collecting supernatant, concentrating the collected supernatant, precipitating with ethanol, and drying in vacuum to constant weight to obtain the yeast cell wall polysaccharide.
As shown in fig. 2, the preparation of the isatis root extract provided by the embodiment of the present invention includes:
s201, placing the isatis root in sterilized deionized water to decoct for three times;
s202, after decoction, putting the decoction on inorganic ceramic membrane filtering equipment or organic tubular ultrafiltration membrane filtering equipment for normal temperature filtration, and combining filtrates to obtain a crude extract;
s203, placing the crude extract on a three-leg centrifuge for centrifugation, and performing nanofiltration on nanofiltration membrane filtration equipment to obtain a retentate, namely the isatis root extract.
The first decoction provided by the embodiment of the invention is added with 8 times of sterilized deionized water by weight, and the decoction time is 80-120 min; adding 6 times of sterilized deionized water for the second decoction, wherein the decoction time is 40-60 min; and adding 3 times of sterilized deionized water by weight into the third decoction, wherein the decoction time is 15-30 min.
The centrifugation method provided by the embodiment of the invention comprises the following steps: and (3) placing the crude extract on a three-leg centrifuge with the rotating speed of 6000-8000 turns for centrifuging for 20-35 min.
As shown in fig. 3, the preparation of the licorice extract provided by the embodiment of the present invention includes:
s301, mixing Glycyrrhiza glabra and 85-90% of ethanol according to a weight ratio of 1: 10-12, mixing and then stirring at high speed;
s302, placing the crushed glycyrrhiza glabra in ultrasonic waves for ultrasonic treatment, and performing vacuum filtration to obtain filtrate;
s303, recovering ethanol from the filtrate under reduced pressure, concentrating, and spray drying to obtain the Glycyrrhrizae radix extract.
The rotating speed during high-speed stirring provided by the embodiment of the invention is 3000-6000 rmp, and the working frequency during ultrasonic treatment is 500-1000 Hz.
As shown in fig. 4, the preparation of the astragalus extract provided by the embodiment of the present invention includes:
s401, crushing astragalus, and sieving with a 30-40-mesh sieve to obtain coarse astragalus powder;
s402, adding 0.5-2 times of edible alcohol and an extracting agent into the astragalus coarse powder in an extraction kettle for infiltration and extraction, and then placing the extract in a separation kettle for evaporation and recovery of the extracting agent to obtain astragalus coarse extract;
s403, concentrating the astragalus crude extract under reduced pressure until the ethanol content in the concentrated solution is less than 5%, and performing spray drying to obtain the astragalus extract.
The pressure for the reduced pressure concentration provided by the embodiment of the invention is 0.06-0.12 Mpa, the temperature is 55-85 ℃, and the time for the reduced pressure concentration is 10-20 min.
As shown in fig. 5, the preparation of the complex microbial inoculum powder provided by the embodiment of the invention comprises the following steps: the method comprises the following steps:
s501, boiling the sterilized deionized water, sequentially adding sucrose, tea leaves and potassium dihydrogen phosphate, boiling the water again, and filtering the tea leaves by using gauze;
s502, naturally cooling to 30 ℃, subpackaging into wide-mouth glass bottles, adding a composite microbial inoculum mother solution obtained by mixing lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus according to a ratio of 1:2:2, sealing kraft paper, and standing and culturing at 30 ℃ for 15 days to serve as a strain mother solution;
s503, mixing the cassava powder or the corn powder with the wheat bran, spraying strain mother liquor with the weight of 50-100%, and drying to obtain the composite microbial inoculum powder.
The static culture conditions provided by the embodiment of the invention are as follows: and (3) placing the mixture in an environment of 25-35 ℃ for static culture for 7-14 days to obtain the compound Chinese herbal medicine microecological preparation.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A preparation method of a compound Chinese herbal medicine microecological preparation is characterized by comprising the following steps:
step one, obtaining yeast, cleaning the obtained yeast by using deionized water, freezing the cleaned yeast for 3 hours at the temperature of 2-3 ℃, thawing the yeast in water bath at the temperature of 50 ℃, centrifuging the thawed yeast, and repeating for 2 times to obtain a precipitate; performing enzymolysis autolysis on the precipitate, centrifuging, collecting supernatant, concentrating the collected supernatant, precipitating with ethanol, and vacuum drying to constant weight to obtain yeast cell wall polysaccharide;
step two, preparing the composite microbial inoculum powder: boiling the sterilized deionized water, sequentially adding sucrose, tea leaves and potassium dihydrogen phosphate, boiling the water again, and filtering out the tea leaves by using gauze; naturally cooling to 30 ℃, subpackaging in a wide-mouth glass bottle, adding a mother solution of a composite microbial inoculum prepared by mixing lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus according to a ratio of 1:2:2, sealing kraft paper, and standing and culturing at 30 ℃ for 15 days to serve as a strain mother solution; mixing the cassava powder or the corn powder with wheat bran, spraying strain mother liquor with the weight of 50-100%, and drying to obtain composite microbial inoculum powder for later use;
step three, placing the isatis root in sterilized deionized water for decocting for three times; after the decoction is finished, putting the decoction on inorganic ceramic membrane filtering equipment or organic tubular ultrafiltration membrane filtering equipment for filtering at normal temperature, and combining the filtrates to obtain a crude extract; centrifuging the crude extractive solution in a three-leg centrifuge, and performing nanofiltration on nanofiltration membrane filtration equipment to obtain retentate which is radix Isatidis extract;
step four, mixing the glycyrrhiza glabra with 85-90% of ethanol according to the weight ratio of 1: 10-12, mixing and then stirring at high speed; placing the crushed glycyrrhiza glabra in ultrasonic waves for ultrasonic treatment, and performing vacuum filtration to obtain filtrate; recovering ethanol from the filtrate under reduced pressure, concentrating, and spray drying to obtain Glycyrrhrizae radix extract;
step five, crushing the astragalus, and sieving the crushed astragalus through a sieve of 30-40 meshes to obtain coarse astragalus powder; adding 0.5-2 times of edible alcohol and an extracting agent into the astragalus coarse powder in an extraction kettle for infiltration and extraction, and then putting the extract into a separation kettle for evaporation and recovery of the extracting agent to obtain astragalus coarse extract; concentrating the radix astragali crude extract under reduced pressure until the ethanol content in the concentrated solution is less than 5%, and spray drying to obtain radix astragali extract;
step six, weighing the isatis root extract, the liquorice extract, the astragalus extract, the poria cocos, the chrysanthemum, the fructus forsythiae, the honeysuckle, the yeast cell wall polysaccharide, the composite microbial inoculum, the salt and the sterilized deionized water in sequence according to the mass parts for later use;
and seventhly, crushing the poria cocos, the chrysanthemum, the fructus forsythiae and the honeysuckle, sequentially adding the radix isatidis extract, the liquorice extract, the astragalus extract, the yeast cell wall polysaccharide and the salt, inoculating 25-50% of composite microbial agent powder, adding sterilized deionized water, stirring uniformly, and standing and culturing to obtain the composite Chinese herbal medicine microecological preparation.
2. The method for preparing the composite Chinese herbal medicine microecological preparation according to claim 1, wherein in the first step, the centrifugation comprises: centrifuging for 15min at the speed of 1000-2000r/min by using a centrifugal device.
3. The preparation method of the compound Chinese herbal medicine microecological preparation according to claim 1, wherein in the third step, 8 times of sterilized deionized water by weight is added for the first decoction, and the decoction time is 80-120 min; adding 6 times of sterilized deionized water for the second decoction, wherein the decoction time is 40-60 min; and adding 3 times of sterilized deionized water by weight into the third decoction, wherein the decoction time is 15-30 min.
4. The method for preparing the compound Chinese herbal medicine microecological preparation according to claim 1, wherein in the third step, the centrifugation method comprises the following steps: and (3) placing the crude extract on a three-leg centrifuge with the rotating speed of 6000-8000 turns for centrifuging for 20-35 min.
5. The method for preparing the composite Chinese herbal medicine microecological preparation according to claim 1, wherein in the fourth step, the rotation speed during high-speed stirring is 3000-6000 rmp, and the working frequency during ultrasonic treatment is 500-1000 Hz.
6. The method for preparing the composite Chinese herbal medicine microecological preparation according to claim 1, wherein in the fifth step, the pressure for the concentration under reduced pressure is 0.06-0.12 Mpa, the temperature is 55-85 ℃, and the time for the concentration under reduced pressure is 10-20 min.
7. The method for preparing the compound Chinese herbal medicine microecological preparation according to claim 1, wherein in the seventh step, the static culture conditions are as follows: and (3) placing the mixture in an environment of 25-35 ℃ for static culture for 7-14 days to obtain the compound Chinese herbal medicine microecological preparation.
8. A compound Chinese herbal medicine microecological preparation prepared by the preparation method of the compound Chinese herbal medicine microecological preparation according to any one of claims 1 to 7, which is characterized by comprising, by mass, 20 to 40 parts of an isatis root extract, 15 to 30 parts of a licorice extract, 15 to 20 parts of an astragalus extract, 10 to 15 parts of poria cocos, 8 to 15 parts of chrysanthemum, 6 to 12 parts of fructus forsythiae, 5 to 10 parts of honeysuckle, 10 to 20 parts of yeast cell wall polysaccharide, 3 to 5 parts of a compound microbial inoculum, 0.2 to 0.3 part of salt and the balance of sterilized deionized water.
9. The compound Chinese herbal medicine microecological preparation according to claim 8, wherein the compound microbial agent is prepared by mixing lactobacillus acidophilus, bacillus licheniformis and streptococcus thermophilus according to the ratio of 1:2: 2.
10. Use of the compound Chinese herbal medicine microecological preparation of any one of claims 8 to 9 in preparation of feed and feed additive for ruminants such as cattle and sheep.
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