CN110771755A - Golden pompano feed capable of improving feed utilization rate and enhancing immunity - Google Patents

Golden pompano feed capable of improving feed utilization rate and enhancing immunity Download PDF

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CN110771755A
CN110771755A CN201911250872.2A CN201911250872A CN110771755A CN 110771755 A CN110771755 A CN 110771755A CN 201911250872 A CN201911250872 A CN 201911250872A CN 110771755 A CN110771755 A CN 110771755A
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meal
feed
extract
parts
taking
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王茂生
刘凯华
苏明
黄灿星
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ZHANJIANG HENGXING CORAL FEED CO Ltd
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ZHANJIANG HENGXING CORAL FEED CO Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention discloses a golden pompano feed for improving feed utilization rate and enhancing immunity, which belongs to the technical field of fish feeds and comprises miscellaneous fish meal, shrimp meal, chicken intestine meal, duck liver meal, conch meat meal, egg shell meal, beef powder, starch, a mushroom concentrated extract, a traditional Chinese medicine additive, a lactobacillus fermentation broth and the like. The golden pomfret feed disclosed by the invention is prepared by taking trash fish meal, shrimp meal, chicken intestine meal, duck liver meal, conch meat meal, egg shell meal and beef powder as raw materials and taking a mushroom concentrated extract and a traditional Chinese medicine additive as auxiliary materials, and mixing and granulating the raw materials to obtain a finished feed product, wherein the feed is rich in protein and vitamin contents, and the feed has moderate proportion of various nutritional components, and can meet the requirements of golden pomfret on various nutrients. The golden pomfret feed disclosed by the invention is simple in preparation process, high in production efficiency and suitable for large-scale production.

Description

Golden pompano feed capable of improving feed utilization rate and enhancing immunity
Technical Field
The invention relates to the technical field of fish feed, in particular to golden pompano feed for improving feed utilization rate and enhancing immunity.
Background
Golden pompano belongs to the class Osteichthyes, order Perciformes, family carangidae, genus Populus, and is known as trachinotus ovatus, commonly known as yellow pompano and golden pompano. The fish body is flat on one side and oval, and the length of the hip fin is slightly equal to that of the second dorsal fin. The head is flat, the tail handle is thin, the body is covered with small round scales, and the falling is not easy. Golden pomfret is one of the famous and precious marine economic fishes in the coastal region of south China, the fish meat of the golden pomfret is white, tender, delicious and tasty, the breeding of the golden pomfret has been successful in the artificial breeding of Guangdong, Guangxi, Fujian, Taiwan, Hainan and the like, and the breeding technology is mature.
The nutrient components of the feed can not only influence the normal growth of the fish, but also influence the immunity and disease resistance of the fish. The purpose of feeding fish with feed is to provide the necessary nutritional requirements for maintaining good health, optimal growth, and optimal yield of the farmed fish. The good nutritional quality and physical properties of the feed are the key to achieving production and economic goals for fish farming techniques.
The existing fish feed generally has the phenomenon of low feed utilization rate, does not have the function of improving the immunity of golden pompano, and cannot meet the requirement of modern golden pompano culture.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide the golden pompano feed for improving the feed utilization rate and enhancing the immunity.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A golden pompano feed for improving feed utilization rate and enhancing immunity comprises the following components in parts by weight:
60-80 parts of miscellaneous fish meal, 50-60 parts of shrimp meal, 20-45 parts of chicken intestine meal, 15-30 parts of duck liver meal, 15-30 parts of sea snail meat meal, 30-50 parts of egg shell meal, 15-20 parts of beef powder, 200 parts of starch 150-containing materials, 20-40 parts of mushroom concentrated extract, 20-40 parts of traditional Chinese medicine additive, 80-100 parts of lactobacillus fermentation liquor and 8-10 parts of aspergillus oryzae powder.
Further, the shrimp meal is prepared from high-fat shrimps.
Further, the conch meat powder is prepared from sea snails for removing parasites.
Further, the preparation method of the mushroom concentrated extract comprises the following steps:
taking mushroom entity dry powder according to the mass ratio of 1: 30, adding distilled water, mixing, leaching, repeatedly leaching once, filtering, combining filtrates, centrifuging, taking supernate A, concentrating under reduced pressure, taking the concentrate under reduced pressure according to the mass ratio of 1: 9, adding absolute ethyl alcohol, standing, centrifuging to obtain a supernatant B and a precipitate A, washing the precipitate A with absolute ethyl alcohol, and drying in vacuum to obtain a crude extract, wherein the mass ratio of the crude extract to the precipitate A is 1: 46-50, adding distilled water, mixing, adjusting pH to 6.5-6.7, adding papain with the mass of 10-20% of that of the crude extract, performing enzymolysis at 60-65 ℃ for 3-4h, inactivating enzyme, and taking enzyme inactivation liquid according to the mass ratio of 16: 3: 1, adding chloroform and n-butanol, oscillating, mixing, centrifuging, and taking supernatant C according to a mass ratio of 1: 4 adding absolute ethyl alcohol, mixing, standing, taking the precipitate B, and performing rotary evaporation to obtain an extract;
taking the supernatant B according to the mass ratio of 1: 40, adding distilled water, mixing, keeping at 50-60 ℃ for 3-4h, and taking an extracting solution according to a mass ratio of 1: 8, adding absolute ethyl alcohol, standing, centrifuging, taking supernate D, concentrating under reduced pressure to obtain concentrated solution, and taking the concentrated solution and the extract according to the mass ratio of 5: 3: 1: 10 adding cane sugar and distilled water, mixing to obtain a fermentation culture medium, inoculating 2% of inoculum size of dry saccharomyces cerevisiae into a seed culture medium for culture to obtain a culture solution, inoculating 5% of inoculum size of the culture solution into the fermentation culture medium for fermentation, filtering fermentation liquor, filtering filtrate by using a microfiltration membrane, collecting filtrate, passing the filtrate through ion exchange resin, concentrating under reduced pressure, and drying in vacuum to obtain the concentrated extract of the shiitake mushrooms.
Further, the traditional Chinese medicine additive is a mixture of pseudo-ginseng powder, honeysuckle extract, cacumen biotae extract and Chinese rose extract, wherein the weight ratio of the pseudo-ginseng powder to the honeysuckle extract to the cacumen biotae extract to the Chinese rose extract is 1: 0.8: 1.4: 2.
further, the preparation method of the lactobacillus fermentation liquor comprises the following steps: inoculating the lactobacillus mixed solution into a fermentation culture medium according to the inoculation amount of 1.8-2.2 wt%, wherein the fermentation culture medium comprises the following raw materials: 45-55g/L of molasses, 8-12g/L of corn steep liquor dry powder and 1-3g/L of diammonium hydrogen phosphate, and standing and culturing at 37 ℃ for 15-17h after inoculation to obtain the lactobacillus fermentation liquor.
Further, the preparation method of the lactic acid bacteria mixed solution comprises the following steps: inoculating lactobacillus plantarum into broth culture medium according to the inoculum size of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus plantarum seed solution; inoculating lactobacillus casei into broth culture medium according to the inoculation amount of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus casei seed solution; inoculating lactobacillus acidophilus into MRS broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus acidophilus seed solution, inoculating lactobacillus rhamnosus into broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus rhamnosus seed solution; mixing lactobacillus plantarum seed liquid, lactobacillus casei seed liquid, lactobacillus acidophilus seed liquid and lactobacillus rhamnosus seed liquid according to the weight ratio of 2:1: 1.5 to obtain the lactic acid bacteria mixed solution.
Further, the phagostimulant is a mixture of sesame bran, sweet wine lees, glucose and earthworm powder, wherein the mass ratio of the sesame bran to the sweet wine lees to the glucose to the earthworm powder is 1:1:1: 1.
Further, the preparation method of the golden pomfret feed comprises the following steps:
s1, preparing raw materials of each component according to parts by weight;
s2, putting the miscellaneous fish meal, the shrimp meal, the chicken intestine meal, the duck liver meal, the conch meat meal, the egg shell meal, the beef meal, the mushroom concentrated extract and the traditional Chinese medicine additive into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;
s3, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 36-40%, uniformly mixing, and carrying out sealed anaerobic fermentation culture at 20-30 ℃ for 18-22 days to obtain a fermented mixed material;
s4, taking out the fermented mixed material, adding starch, fully mixing, feeding into a granulator for granulation, drying in the sun to form feed particles, packaging and refrigerating to obtain the finished product of the golden pompano feed.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) the golden pomfret feed disclosed by the invention is prepared by taking trash fish meal, shrimp meal, chicken intestine meal, duck liver meal, conch meat meal, egg shell meal and beef powder as raw materials and taking a mushroom concentrated extract and a traditional Chinese medicine additive as auxiliary materials, and mixing and granulating the raw materials to obtain a finished feed product, wherein the feed is rich in protein and vitamin contents, and the feed has moderate proportion of various nutritional components, and can meet the requirements of golden pomfret on various nutrients.
(2) The traditional Chinese medicine additive prepared from the pseudo-ginseng powder, the honeysuckle extract, the cacumen biotae extract and the Chinese rose extract is added into the golden pompano appetite-stimulating and disease-preventing traditional Chinese medicine composition, so that the appetite of the golden pompano can be effectively promoted, and meanwhile, the disease-preventing effect is effectively achieved.
(3) The invention also adds the mushroom concentrated extract, the mushroom can remove monosaccharide and sucrose through yeast fermentation of oligosaccharide and polysaccharide mixed solution, the oligosaccharide mainly comprises trehalose, the loss is basically avoided in the fermentation process, the trehalose is taken as a bait additive to be orally taken to form prawn immunity, the immune system of the prawn is stimulated, the antibacterial activity and the superoxide dismutase activity are improved, the oxidation resistance of the prawn body is promoted, and the immune capacity of the prawn is improved.
(4) According to the golden pomfret feed, aspergillus oryzae powder and lactobacillus fermentation liquor are added to carry out anaerobic fermentation on the golden pomfret feed, acid substances are generated through lactobacillus fermentation, an acid environment is provided to inhibit the growth of mixed bacteria, and the aspergillus oryzae produces amylase, protease, pectinase, kojic acid and the like.
(5) The golden pomfret feed disclosed by the invention is simple in preparation process, high in production efficiency and suitable for large-scale production.
Drawings
FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1:
a golden pompano feed for improving feed utilization rate and enhancing immunity comprises the following components in parts by weight:
60-80 parts of miscellaneous fish meal, 50-60 parts of shrimp meal, 20-45 parts of chicken intestine meal, 15-30 parts of duck liver meal, 15-30 parts of sea snail meat meal, 30-50 parts of egg shell meal, 15-20 parts of beef powder, 200 parts of starch 150-containing materials, 20-40 parts of mushroom concentrated extract, 20-40 parts of traditional Chinese medicine additive, 80-100 parts of lactobacillus fermentation liquor and 8-10 parts of aspergillus oryzae powder.
The shrimp powder is prepared from high-fat shrimps.
The conch meat powder is prepared from sea snails with parasite removal.
The preparation method of the mushroom concentrated extract comprises the following steps:
taking mushroom entity dry powder according to the mass ratio of 1: 30, adding distilled water, mixing, leaching, repeatedly leaching once, filtering, combining filtrates, centrifuging, taking supernate A, concentrating under reduced pressure, taking the concentrate under reduced pressure according to the mass ratio of 1: 9, adding absolute ethyl alcohol, standing, centrifuging to obtain a supernatant B and a precipitate A, washing the precipitate A with absolute ethyl alcohol, and drying in vacuum to obtain a crude extract, wherein the mass ratio of the crude extract to the precipitate A is 1: 46-50, adding distilled water, mixing, adjusting pH to 6.5-6.7, adding papain with the mass of 10-20% of that of the crude extract, performing enzymolysis at 60-65 ℃ for 3-4h, inactivating enzyme, and taking enzyme inactivation liquid according to the mass ratio of 16: 3: 1, adding chloroform and n-butanol, oscillating, mixing, centrifuging, and taking supernatant C according to a mass ratio of 1: 4 adding absolute ethyl alcohol, mixing, standing, taking the precipitate B, and performing rotary evaporation to obtain an extract;
taking the supernatant B according to the mass ratio of 1: 40, adding distilled water, mixing, keeping at 50-60 ℃ for 3-4h, and taking an extracting solution according to a mass ratio of 1: 8, adding absolute ethyl alcohol, standing, centrifuging, taking supernate D, concentrating under reduced pressure to obtain concentrated solution, and taking the concentrated solution and the extract according to the mass ratio of 5: 3: 1: 10 adding cane sugar and distilled water, mixing to obtain a fermentation culture medium, inoculating 2% of inoculum size of dry saccharomyces cerevisiae into a seed culture medium for culture to obtain a culture solution, inoculating 5% of inoculum size of the culture solution into the fermentation culture medium for fermentation, filtering fermentation liquor, filtering filtrate by using a microfiltration membrane, collecting filtrate, passing the filtrate through ion exchange resin, concentrating under reduced pressure, and drying in vacuum to obtain the concentrated extract of the shiitake mushrooms.
The traditional Chinese medicine additive is a mixture of pseudo-ginseng powder, honeysuckle extract, cacumen biotae extract and Chinese rose extract, wherein the weight ratio of the pseudo-ginseng powder to the honeysuckle extract to the cacumen biotae extract to the Chinese rose extract is 1: 0.8: 1.4: 2.
the preparation method of the lactobacillus fermentation liquor comprises the following steps: inoculating the lactobacillus mixed solution into a fermentation culture medium according to the inoculation amount of 1.8-2.2 wt%, wherein the fermentation culture medium comprises the following raw materials: 45-55g/L of molasses, 8-12g/L of corn steep liquor dry powder and 1-3g/L of diammonium hydrogen phosphate, and standing and culturing at 37 ℃ for 15-17h after inoculation to obtain the lactobacillus fermentation liquor.
The preparation method of the lactic acid bacteria mixed solution comprises the following steps: inoculating lactobacillus plantarum into broth culture medium according to the inoculum size of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus plantarum seed solution; inoculating lactobacillus casei into broth culture medium according to the inoculation amount of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus casei seed solution; inoculating lactobacillus acidophilus into MRS broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus acidophilus seed solution, inoculating lactobacillus rhamnosus into broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus rhamnosus seed solution; mixing lactobacillus plantarum seed liquid, lactobacillus casei seed liquid, lactobacillus acidophilus seed liquid and lactobacillus rhamnosus seed liquid according to the weight ratio of 2:1: 1.5 to obtain the lactic acid bacteria mixed solution.
The phagostimulant is a mixture of sesame bran, sweet vinasse, glucose and earthworm powder, wherein the mass ratio of the sesame bran to the sweet vinasse to the glucose to the earthworm powder is 1:1:1: 1.
Referring to fig. 1, the preparation method of the golden pomfret feed comprises the following steps:
s1, preparing raw materials of each component according to parts by weight;
s2, putting the miscellaneous fish meal, the shrimp meal, the chicken intestine meal, the duck liver meal, the conch meat meal, the egg shell meal, the beef meal, the mushroom concentrated extract and the traditional Chinese medicine additive into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;
s3, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 36-40%, uniformly mixing, and carrying out sealed anaerobic fermentation culture at 20-30 ℃ for 18-22 days to obtain a fermented mixed material;
s4, taking out the fermented mixed material, adding starch, fully mixing, feeding into a granulator for granulation, drying in the sun to form feed particles, packaging and refrigerating to obtain the finished product of the golden pompano feed.
The golden pomfret feed disclosed by the invention is prepared by taking trash fish meal, shrimp meal, chicken intestine meal, duck liver meal, conch meat meal, egg shell meal and beef powder as raw materials and taking a mushroom concentrated extract and a traditional Chinese medicine additive as auxiliary materials, and mixing and granulating the raw materials to obtain a finished feed product, wherein the feed is rich in protein and vitamin contents, and the feed has moderate proportion of various nutritional components, and can meet the requirements of golden pomfret on various nutrients.
The traditional Chinese medicine additive prepared from the pseudo-ginseng powder, the honeysuckle extract, the cacumen biotae extract and the Chinese rose extract is added into the golden pompano appetite-stimulating and disease-preventing traditional Chinese medicine composition, so that the appetite of the golden pompano can be effectively promoted, and meanwhile, the disease-preventing effect is effectively achieved.
The invention also adds the mushroom concentrated extract, the mushroom can remove monosaccharide and sucrose through yeast fermentation of oligosaccharide and polysaccharide mixed solution, the oligosaccharide mainly comprises trehalose, the loss is basically avoided in the fermentation process, the trehalose is taken as a bait additive to be orally taken to form prawn immunity, the immune system of the prawn is stimulated, the antibacterial activity and the superoxide dismutase activity are improved, the oxidation resistance of the prawn body is promoted, and the immune capacity of the prawn is improved.
According to the golden pomfret feed, aspergillus oryzae powder and lactobacillus fermentation liquor are added to carry out anaerobic fermentation on the golden pomfret feed, acid substances are generated through lactobacillus fermentation, an acid environment is provided to inhibit the growth of mixed bacteria, and the aspergillus oryzae produces amylase, protease, pectinase, kojic acid and the like.
The golden pomfret feed disclosed by the invention is simple in preparation process, high in production efficiency and suitable for large-scale production.
The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.

Claims (9)

1. A golden pompano feed for improving feed utilization rate and enhancing immunity is characterized in that: the paint comprises the following components in parts by weight:
60-80 parts of miscellaneous fish meal, 50-60 parts of shrimp meal, 20-45 parts of chicken intestine meal, 15-30 parts of duck liver meal, 15-30 parts of sea snail meat meal, 30-50 parts of egg shell meal, 15-20 parts of beef powder, 200 parts of starch 150-containing materials, 20-40 parts of mushroom concentrated extract, 20-40 parts of traditional Chinese medicine additive, 80-100 parts of lactobacillus fermentation liquor and 8-10 parts of aspergillus oryzae powder.
2. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the shrimp powder is prepared from high-fat shrimps.
3. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the conch meat powder is prepared from sea snails with parasite removal.
4. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the preparation method of the mushroom concentrated extract comprises the following steps:
taking mushroom entity dry powder according to the mass ratio of 1: 30, adding distilled water, mixing, leaching, repeatedly leaching once, filtering, combining filtrates, centrifuging, taking supernate A, concentrating under reduced pressure, taking the concentrate under reduced pressure according to the mass ratio of 1: 9, adding absolute ethyl alcohol, standing, centrifuging to obtain a supernatant B and a precipitate A, washing the precipitate A with absolute ethyl alcohol, and drying in vacuum to obtain a crude extract, wherein the mass ratio of the crude extract to the precipitate A is 1: 46-50, adding distilled water, mixing, adjusting pH to 6.5-6.7, adding papain with the mass of 10-20% of that of the crude extract, performing enzymolysis at 60-65 ℃ for 3-4h, inactivating enzyme, and taking enzyme inactivation liquid according to the mass ratio of 16: 3: 1, adding chloroform and n-butanol, oscillating, mixing, centrifuging, and taking supernatant C according to a mass ratio of 1: 4 adding absolute ethyl alcohol, mixing, standing, taking the precipitate B, and performing rotary evaporation to obtain an extract;
taking the supernatant B according to the mass ratio of 1: 40, adding distilled water, mixing, keeping at 50-60 ℃ for 3-4h, and taking an extracting solution according to a mass ratio of 1: 8, adding absolute ethyl alcohol, standing, centrifuging, taking supernate D, concentrating under reduced pressure to obtain concentrated solution, and taking the concentrated solution and the extract according to the mass ratio of 5: 3: 1: 10 adding cane sugar and distilled water, mixing to obtain a fermentation culture medium, inoculating 2% of inoculum size of dry saccharomyces cerevisiae into a seed culture medium for culture to obtain a culture solution, inoculating 5% of inoculum size of the culture solution into the fermentation culture medium for fermentation, filtering fermentation liquor, filtering filtrate by using a microfiltration membrane, collecting filtrate, passing the filtrate through ion exchange resin, concentrating under reduced pressure, and drying in vacuum to obtain the concentrated extract of the shiitake mushrooms.
5. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the traditional Chinese medicine additive is a mixture of pseudo-ginseng powder, honeysuckle extract, cacumen biotae extract and Chinese rose extract, wherein the weight ratio of the pseudo-ginseng powder to the honeysuckle extract to the cacumen biotae extract to the Chinese rose extract is 1: 0.8: 1.4: 2.
6. the golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the preparation method of the lactobacillus fermentation liquor comprises the following steps: inoculating the lactobacillus mixed solution into a fermentation culture medium according to the inoculation amount of 1.8-2.2 wt%, wherein the fermentation culture medium comprises the following raw materials: 45-55g/L of molasses, 8-12g/L of corn steep liquor dry powder and 1-3g/L of diammonium hydrogen phosphate, and standing and culturing at 37 ℃ for 15-17h after inoculation to obtain the lactobacillus fermentation liquor.
7. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 6, which is characterized in that: the preparation method of the lactic acid bacteria mixed solution comprises the following steps: inoculating lactobacillus plantarum into broth culture medium according to the inoculum size of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus plantarum seed solution; inoculating lactobacillus casei into broth culture medium according to the inoculation amount of 0.8-1.2 wt%, and culturing at 37 deg.C for 20-24 hr to obtain lactobacillus casei seed solution; inoculating lactobacillus acidophilus into MRS broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus acidophilus seed solution, inoculating lactobacillus rhamnosus into broth culture medium according to the inoculum size of 0.8-1.2 wt%, culturing at 37 deg.C for 20-24h to obtain lactobacillus rhamnosus seed solution; mixing lactobacillus plantarum seed liquid, lactobacillus casei seed liquid, lactobacillus acidophilus seed liquid and lactobacillus rhamnosus seed liquid according to the weight ratio of 2:1: 1.5 to obtain the lactic acid bacteria mixed solution.
8. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the phagostimulant is a mixture of sesame bran, sweet vinasse, glucose and earthworm powder, wherein the mass ratio of the sesame bran to the sweet vinasse to the glucose to the earthworm powder is 1:1:1: 1.
9. The golden pompano feed for improving feed utilization rate and enhancing immunity according to claim 1, which is characterized in that: the preparation method of the golden pomfret feed comprises the following steps:
s1, preparing raw materials of each component according to parts by weight;
s2, putting the miscellaneous fish meal, the shrimp meal, the chicken intestine meal, the duck liver meal, the conch meat meal, the egg shell meal, the beef meal, the mushroom concentrated extract and the traditional Chinese medicine additive into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;
s3, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 36-40%, uniformly mixing, and carrying out sealed anaerobic fermentation culture at 20-30 ℃ for 18-22 days to obtain a fermented mixed material;
s4, taking out the fermented mixed material, adding starch, fully mixing, feeding into a granulator for granulation, drying in the sun to form feed particles, packaging and refrigerating to obtain the finished product of the golden pompano feed.
CN201911250872.2A 2019-12-09 2019-12-09 Golden pompano feed capable of improving feed utilization rate and enhancing immunity Pending CN110771755A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113558153A (en) * 2020-04-29 2021-10-29 广西百跃农牧发展有限公司 Trachinotus ovatus reinforced feed and preparation method thereof
CN114158658A (en) * 2021-12-14 2022-03-11 广州亿盛生物技术有限公司 Trachinotus ovatus feed capable of promoting digestion of low-fish meal and preparation method of Trachinotus ovatus feed

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105495060A (en) * 2015-12-30 2016-04-20 广西万博贸易有限公司 Striped prawn feed
CN105661096A (en) * 2015-12-30 2016-06-15 苏州市相城区太平蟹业专业合作社 Fish feed growth additive
CN106417996A (en) * 2016-08-31 2017-02-22 北海保通食品股份有限公司 Gold pomfret feed
CN106616043A (en) * 2016-12-23 2017-05-10 怀宁县鲶鱼湾生态农业有限公司 Novel catfish feed and preparation method thereof
CN108056324A (en) * 2017-12-30 2018-05-22 张静 A kind of shrimp feed and preparation method thereof
CN109845920A (en) * 2019-04-01 2019-06-07 湛江恒兴养殖技术服务有限公司 A kind of fermentation shrimp feed and its preparation method and application

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105495060A (en) * 2015-12-30 2016-04-20 广西万博贸易有限公司 Striped prawn feed
CN105661096A (en) * 2015-12-30 2016-06-15 苏州市相城区太平蟹业专业合作社 Fish feed growth additive
CN106417996A (en) * 2016-08-31 2017-02-22 北海保通食品股份有限公司 Gold pomfret feed
CN106616043A (en) * 2016-12-23 2017-05-10 怀宁县鲶鱼湾生态农业有限公司 Novel catfish feed and preparation method thereof
CN108056324A (en) * 2017-12-30 2018-05-22 张静 A kind of shrimp feed and preparation method thereof
CN109845920A (en) * 2019-04-01 2019-06-07 湛江恒兴养殖技术服务有限公司 A kind of fermentation shrimp feed and its preparation method and application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113558153A (en) * 2020-04-29 2021-10-29 广西百跃农牧发展有限公司 Trachinotus ovatus reinforced feed and preparation method thereof
CN114158658A (en) * 2021-12-14 2022-03-11 广州亿盛生物技术有限公司 Trachinotus ovatus feed capable of promoting digestion of low-fish meal and preparation method of Trachinotus ovatus feed

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Application publication date: 20200211