CN110150457A - A kind of antibacterial type yeast culture and its application - Google Patents
A kind of antibacterial type yeast culture and its application Download PDFInfo
- Publication number
- CN110150457A CN110150457A CN201811120173.1A CN201811120173A CN110150457A CN 110150457 A CN110150457 A CN 110150457A CN 201811120173 A CN201811120173 A CN 201811120173A CN 110150457 A CN110150457 A CN 110150457A
- Authority
- CN
- China
- Prior art keywords
- yeast culture
- fermentation
- culture
- preparation
- antibacterial type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
Abstract
The present invention relates to a kind of antibacterial type yeast culture and its application, the yeast culture on S. cervisiae HKB-36 under given conditions defined medium by sufficiently fermenting.Yeast culture of the invention can significantly inhibit the growth of the harmful bacterias such as Escherichia coli, staphylococcus aureus, salmonella, be particularly useful in enhancing the intestinal health of livestock and poultry and improve growth performance, reduce the use of antibiotic in feed.
Description
Technical field
The present invention relates to fungi field, in particular to a kind of antibacterial type yeast culture and its application.
Background technique
China as cultivation big country, cultivation amount and the livestock products world quantity Jun Zhan forefront, but be faced with epidemic disease take place frequently, feed
The problems puzzlement such as abuse of antibiotics and Animal product quality decline.The antibiotic consumption figure of China accounts for about global total quantity consumed
50%, annual about 110,000 tons, wherein aquaculture accounts for about the 60% of the total dosage of national antibiotic with 6.5 ten thousand tons of antibiotic.Antibiosis
The excessive of element has made China become one of global antibiotic resistance problem country the most serious.
In consideration of it, the country on the 5th of August in 2016 defends 14 ministries and commissions such as planning commission, National Development and Reform Committee, the Department of Science and Technology, Ministry of Agriculture joint hair
Cloth " containment bacterial resistance national plan of action (2016-2020) " (state defends doctor's hair (2016) 43).Stringent limitation antibacterial
Use of the element in aquaculture is to contain one of bacterial drug resistance sprawling, the most important measure for ensuring " people doctor has medicine available ".
The Ministry of Agriculture provided from April 30th, 2017, forbade use of the colistine sulfate in feed.Agricultural in April, 2018 rural area
Portion discloses " Antibiotic use minimizing action pilot work scheme (2018-2021) for animals.Scheme is pointed out to strive passing through
3 years implemented cultivating link Antibiotic use minimizing action pilot work for animals, and medicated feed additive will be in the year two thousand twenty
All exit.This action is a large impact to China's animal husbandry production efficiency, and sufficiently exploitation can promote livestock birds health water
Feed resource that is flat, improving production performance is extremely urgent.
Yeast culture be one kind under specific process conditions by saccharomycete specific culture medium (such as corn, wheat bran and
The cereal such as oat) on the Tiny ecosystem product that is formed after sufficient anaerobic fermentation, mainly by being extracellularly metabolized product, by hair
The culture medium and a small amount of inactive yeast cells to make a variation after ferment is constituted.Due to its be rich in protein, and containing peptides,
Organic acid, oligosaccharides, flavour enhancing substance, aromatic substance and unknown growth factor, improve feed palatability, improve animal feed intake,
Improve and plays a significant role in animal intestinal tract health and growth.In recent years, Yeast Cultivation increasingly by domestic and international feed and
The attention of aquaculture.But in the prior art, the quality height of yeast culture mainly emphasizes its peptides, organic acid, flavour enhancing object
The content of the ingredients such as matter, aromatic substance and unknown growth factor, also not about the report with bacteriostatic activity yeast culture.
Also, the Yeast Cultivation product in China is mostly external introduction, national related research relative poor at this stage.How yeast is improved
The product quality of culture improves its function and effect, and reducing its production cost is current studies in China urgent problem to be solved.
Summary of the invention
The purpose of the present invention is in order to solve the above problem, the present invention provides an Accharomyces cerevisiae bacterium HKB-36 and its application.
According to the first aspect of the invention, a kind of antibacterial type yeast culture is provided, the yeast culture is by the wine brewing ferment
Female bacterium HKB-36 anaerobic fermentation forms.
According to the second aspect of the invention, application of the yeast culture in terms of inhibiting bacterial growth is provided.
Wherein, bacterium includes Escherichia coli, staphylococcus aureus and salmonella.
According to the third aspect of the invention we, the preparation method of antibacterial type yeast culture is provided, which includes oblique
Face actication of culture, seed liquor preparation, liquid aerobic fermentation and solid anaerobic fermentation step:
Wherein, in seed liquor preparation step, fluid nutrient medium includes that malt leaches powder 145g/L, chloramphenicol 0.08g/L, liquid
Body Medium's PH Value is 5.8-6.8.
Wherein, in seed liquor preparation step, cultivation temperature is 28 DEG C -36 DEG C, revolving speed 120-200r/min；Incubation time
For 18-30h.
Wherein, in liquid aerobic fermentation step, fluid nutrient medium includes that malt leaches powder 145g/L, chloramphenicol 0.08g/L,
Fluid nutrient medium pH value is 5.8-6.8.
Wherein, in liquid aerobic fermentation step, fermentation temperature is 28 DEG C -36 DEG C, fermentation time 24-36h, and ventilatory capacity is
1-4L/min。
Wherein, in solid anaerobic fermentation step, fermentation medium includes 2-4 parts of vinasse, 2-4 parts of maize peel, 1-3 parts of wheat bran
With 1-3 parts of apple pomace, culture medium carbon-nitrogen ratio be 2-7:1, moisture content 35%-70%.
Wherein, in solid anaerobic fermentation step, fermentation temperature is 28 DEG C -36 DEG C, fermentation time 18-48h.
Wherein, which further includes the step for preparing fermentation culture medium finished product after strain liquid solid anaerobic fermentation
It is rapid:
Fermentation temperature is adjusted to 60 DEG C -75 DEG C, broken wall is inactivated to the S. cervisiae HKB-36, then crushes drying,
Yeast culture finished product is made.
1. the yeast culture of S. cervisiae HKB-36 provided by the invention can significantly inhibit Escherichia coli, golden yellow Portugal
The growth of the harmful bacterias such as grape coccus, salmonella is particularly useful in enhancing the intestinal health of livestock and poultry and improves growth performance, reduces
The use of antibiotic in feed.
2. in addition to having bacteriostasis, yeast culture prepared by the present invention, thick protein and mannan content it is high and
Stablize, for gross protein value up to 28% or more, mannan content is higher than 0.5%
3. antibacterial type yeast culture or a kind of feedstuff with high nutritive value of the invention, can improve feed
Palatability improves animal feed intake and improves feed nutrient digestibility；And it is exactly with the maximum difference of common yeast culture
With antibacterial activity, the significant effect for improving animal intestinal tract health and growth performance can be played；Raw material (vinasse, jade of use
Rice skin, wheat bran and apple pomace) it is low in cost, these resources are made full use of, high added value is created.
4. the present invention uses liquid-solid combined ferment preparation process, it is antibacterial to develop that function and effect are good, price is low
Type yeast culture, process is simple, easy to operate.
Detailed description of the invention
By reading the following detailed description of the preferred embodiment, various other advantages and benefits are common for this field
Technical staff will become clear.The drawings are only for the purpose of illustrating a preferred embodiment, and is not considered as to the present invention
Limitation.And throughout the drawings, the same reference numbers will be used to refer to the same parts.In the accompanying drawings:
Fig. 1 shows the colonial morphology electron microscopic picture of the S. cervisiae HKB-36 of embodiment according to the present invention；
Fig. 2 shows the development tree graph pieces of the S. cervisiae HKB-36 of embodiment according to the present invention；
Fig. 3 shows the fungistatic effect figure of the fermentating metabolism product of the different S. cervisiaes of embodiment according to the present invention
Piece；
Fig. 4 shows the fungistatic effect figure of the yeast culture of the different S. cervisiaes of embodiment according to the present invention
Piece；
Fig. 5 shows the antibacterial type yeast culture of the S. cervisiae HKB-36 of embodiment according to the present invention to wean
The influence picture of piglet growth performance；
Fig. 6 shows the antibacterial type yeast culture of the S. cervisiae HKB-36 of embodiment according to the present invention to wean
The influence picture of grice diarrhoea.
Specific embodiment
The illustrative embodiments of the disclosure are more fully described below with reference to accompanying drawings.Although showing this public affairs in attached drawing
The illustrative embodiments opened, it being understood, however, that may be realized in various forms the disclosure without the reality that should be illustrated here
The mode of applying is limited.It is to be able to thoroughly understand the disclosure on the contrary, providing these embodiments, and can be by this public affairs
The range opened is fully disclosed to those skilled in the art.
The separation screening of 1 S. cervisiae HKB-36 of embodiment
(1) culture medium: malt extract medium.500g malt is taken, is put into beaker after being crushed, 2000mL distillation is added
Water stirs lower 45 DEG C of water-bath 30min, and temperature is turned up to 70 DEG C, keeps 1h；8 layers of filtered through gauze, and with positive Liu Shu wash beaker and
Filter pocket makes filtrate reach 2000mL, after cooling, 10 DEG C save backup after boiling.
(2) separation of saccharomycete: with beer, grape wine, pickles, fermented fruits and vegetables juice, dough and koumiss etc. for raw material, point
Not Cai Ji 400mL or 400g material sample (smashing to pieces for solid), set in sterile conical flasks, 8 layers of gauze of lid in 25 DEG C -30 DEG C from
So fermentation 2-3d.Under aseptic condition, fermentation culture sample is shaken up, draws appropriate fermentation liquid in by going out with sterile pipette
In the brewer's wort culture solution of bacterium, 25 DEG C of cultures.Gradient dilution method is used to be diluted to concentration as 10 when having vinosity-4With 10-5, inhale
Culture solution after taking 0.1-0.2mL to dilute is spread evenly across on malt extract medium (+2% agar of brewer's wort culture solution), and 25 DEG C
It is inverted 48h, after growing bacterium colony, selection has typical single colonie (saccharomycete bacterium colony) further scribing line purifying 2-3 times, through microscopy
To be transferred in brewer's wort solid slope culture medium respectively after pure culture, 4 DEG C are saved backup.
(3) yeast screening assay: being cultivated by malt extract medium, observation period growing state, selects survival rate highest, life
Long most luxuriant bacterium carries out the bacteriostatic test screening of next step.Using Odontothrips loti, culture have Escherichia coli, salmonella,
It is separately added into the saccharomycete saved backup in the culture medium of staphylococcus glucose coccus, different saccharomycete are against three plants of harmful bacterias
Effect such as table 1 is killed in suppression, selects the optimal single bacterial strain of antibacterial/bacteriostatic activity, is named as HKB-36.
Table 1: different saccharomycete kill test to the suppression of Escherichia coli, salmonella and staphylococcus aureus
Bacterial strain identification:
1. colony characteristics: being cultivated three days for 25 DEG C in malt juice liquid medium, cell spherical shape, ellipse, sausage shape, size
For (5.6-9.3) × (4.3-5.2) μm, there is precipitating to be formed.Wort agar inclined-plane culture 1 month, bacterium colony cheese shape are milky white
Color, surface is smooth, neat in edge.Corn agar Dalmau plate culture, does not generate pseudohypha, specific as shown in Figure 1.
2. bacterial strain Phylogenetic Analysis
The HKB-36 single colonie for choosing purifying is inoculated in the screening and culturing medium that pH is 5.8, in 28 DEG C of constant-temperature table cultures,
Revolving speed 120r/min；30h is cultivated, the ITS sequence of PCR amplification bacterial strain is then done using its bacterium solution as template, is sequenced, and will be surveyed
Sequence result analyzes software with Molecular Evolutionary Genetics and does its development tree, specific as shown in Fig. 2, discovery HKB-36 and registered wine brewing
The sequence homology highest of saccharomycete Saccharomyces cerevisiae, reaches 99%, it is thus determined that it is saccharomyces cerevisiae
Pseudomonas Saccharomyces cerevisiae.
4. Molecular Identification: the ITS sequence of the HKB-36 of measurement is as shown in SED ID NO:1, by it in http: //
Sequence alignment is carried out on the website archive-dtd.ncbi.nlm.nih.gov/, is analyzed based on homogeneous angular, S. cervisiae
HKB-36 is the newcomer of saccharomyces cerevisiae Pseudomonas, is named as S. cervisiae Saccharomyces cerevisiae
HKB-36。
The preparation of 2 antibacterial type yeast culture 1 of embodiment
(1) slant strains activate: by the saccharomyces cerevisiae HKB-36 of freezing be inoculated into containing malt leach powder 145g/L,
On the slant medium of chloramphenicol 0.08g/L and agar 2%, 30 DEG C of constant temperature are stood, cultivates 48h, the saccharomycete after being activated
Kind.
(2) preparation of seed liquor: the barms after activation are seeded to 1 ring and leach powder 145g/L, chlorine containing malt
Mycin 0.08g/L, pH value be 5.8 fluid nutrient medium in, in 28 DEG C of constant-temperature table cultures, revolving speed 200r/min；Cultivate 18h.
(3) liquid aerobic fermentation: the seed liquor prepared is inoculated into 15% inoculum concentration and is soaked equipped with new containing malt
Powder 145g/L, chloramphenicol 0.08g/L out, pH value expand culture in the fermentor for 6.8 fluid nutrient medium, control ventilation
Amount is 4L/min, and temperature is 28 DEG C, fermentation time 36h.Obtain expanding the liquid spawn liquid of culture.
(4) liquid spawn liquid solid anaerobic fermentation: is seeded to mass fraction as 2 parts of vinasse, jade using 25% inoculum concentration
In 4 parts of skin of rice, 3 parts of wheat bran and 1 part of apple pomace of sterile solid culture medium, addition urea control carbon-nitrogen ratio is 2:1, in controllable temperature
Anaerobic fermentation is carried out in fermentor, control moisture content is 70%, and fermentation temperature is 36 DEG C, fermentation time 18h.Obtain solid hair
Ferment product.
(5) acquisition of yeast culture product: after solid fermentation, the temperature of fermentor is adjusted to 60 DEG C, to culture
Saccharomycete in object carries out inactivation and broken wall, it is made sufficiently to discharge the substances such as mannosan, the nucleic acid in yeast cell contents,
Inactivate broken wall 1h, then culture is transferred in air-flow crushing drying machine crush it is dry to get to yeast culture at
Product.
The preparation of 3 antibacterial type yeast culture 2 of embodiment
(1) slant strains activate: by the saccharomyces cerevisiae HKB-36 of freezing be inoculated into containing malt leach powder 145g/L,
On the slant medium of chloramphenicol 0.08g/L and agar 2%, 30 DEG C of constant temperature are stood, cultivates 48h, the saccharomycete after being activated
Kind.
(2) preparation of seed liquor: the barms after activation are seeded to 1 ring and leach powder 145g/L, chlorine containing malt
Mycin 0.08g/L, pH value be 6.8 fluid nutrient medium in, in 36 DEG C of constant-temperature table cultures, revolving speed 120r/min；Cultivate 30h.
(3) liquid aerobic fermentation: the seed liquor prepared is inoculated into 15% inoculum concentration and is soaked equipped with new containing malt
Powder 145g/L, chloramphenicol 0.08g/L out, pH value expand culture in the fermentor for 5.8 fluid nutrient medium, control ventilation
Amount is 1L/min, and temperature is 36 DEG C, and fermentation time is for 24 hours.Obtain expanding the liquid spawn liquid of culture.
(4) liquid spawn liquid solid anaerobic fermentation: is seeded to mass fraction as 4 parts of vinasse, jade using 25% inoculum concentration
In 2 parts of skin of rice, 1 part of wheat bran and 3 parts of apple pomace of sterile solid culture medium, addition urea control carbon-nitrogen ratio is 7:1, in controllable temperature
Anaerobic fermentation is carried out in fermentor, control moisture content is 35%, and fermentation temperature is 28 DEG C, fermentation time 48h.Obtain solid hair
Ferment product.
(5) acquisition of yeast culture product: after solid fermentation, the temperature of fermentor is adjusted to 75 DEG C, to culture
Saccharomycete in object carries out inactivation and broken wall, it is made sufficiently to discharge the substances such as mannosan, the nucleic acid in yeast cell contents,
Inactivate broken wall 1h, then culture is transferred in air-flow crushing drying machine crush it is dry to get to yeast culture at
Product.
The antibacterial experiment in vitro of 4 antibacterial type yeast culture of embodiment
It is respectively fermentation strain with other 5 saccharomycetes separated in HKB-36 and embodiment 1 of the invention, according to
Different yeast culture products is prepared in the technique of embodiment 2.Bacteriostatic test is carried out using Odontothrips loti, observation is every respectively
Saccharomycete is to the fungistatic effect of Escherichia coli, staphylococcus aureus and salmonella, and in addition to using physiological saline as sky
White control increases ampicillin as positive control.
Experimental result is as shown in Figure 4.In Fig. 4, d3 indicates the resulting yeast of S. cervisiae HKB-36 fermentation of the invention
Culture, d3H, d5, d5H, d10 and d10H are respectively saccharomycete HKB-6, HKB-10, HKB-27, HKB-33 and HKB-46 difference
Ferment resulting yeast culture.Not marking below intermediate ampicillin is physiological saline blank control.
As shown in Figure 4, ampicillin all has good suppression to Escherichia coli, staphylococcus aureus and salmonella
Bacterium effect.The yeast culture that saccharomyces cerevisiae HKB-36 of the present invention is fermented also can obviously inhibit Escherichia coli, golden yellow grape
The growth of coccus and salmonella, fungistatic effect are slightly weaker than ampicillin, and the suppression according to antibacterial circle diameter, to this 3 plants of bacterium
Bacterium effect is Escherichia coli > salmonella > staphylococcus aureus.And the yeast training that the fermentation of other 5 Accharomyces cerevisiaes generates
Object is supported to Escherichia coli, staphylococcus aureus and salmonella almost without bacteriostatic activity.
Influence of the 5 antibacterial type yeast culture of embodiment to Growth Performance of Weaning Piglets
3 week old or so are taken, the weanling pig of average weight 6.8kg or so is 96 total, and every 24 weanling pigs are divided into one
Group, amounts to 4 groups, respectively blank group (feed does not add yeast culture) and is added to 200g/t, 400g/t or 600g/t
The test group of antibacterial type yeast culture of the present invention in embodiment 2.Experimental period 28d measures its average daily gain and feed-weight ratio.
Fig. 5 is that influence of the yeast culture to Growth Performance of Weaning Piglets is added in weanling pig feed as shown in Figure 5
This antibacterial type yeast culture is remarkably improved its average daily gain, and improves its feed-weight ratio, wherein with the addition water of 400g/t
Flat effect is best, and average daily gain, which improves 11.04%, feed-weight ratio compared with control group, reduces by 2.4%.
Influence of the 6 antibacterial type yeast culture of embodiment to diarrhea of weaned piglets
4 week old or so are taken, the weanling pig of average weight 7.8kg or so is 36 total, and every 12 weanling pigs are divided into one
Group, amounts to 3 groups, respectively blank group (feed does not add yeast culture) and is added in the embodiment 3 of 400g/t the present invention
The test group of antibacterial type yeast culture or commercially available common yeast culture.Experimental period 4 weeks, diarrhea of weaned piglets feelings were recorded weekly
Condition.
Fig. 6 is impact analysis figure of the various yeast cultures to diarrhea of weaned piglets.It will be appreciated from fig. 6 that compared with the control group,
Two yeast culture test groups can improve the diarrhea situation of weanling pig, but the effect of antibacterial type yeast culture of the present invention
It is substantially better than commercially available common yeast culture.Also side light, saccharomyces cerevisiae HKB-36 of the present invention are produced during the fermentation for this
Given birth to a large amount of antibacterial substances, yeast culture produced can effective antibacterial weanling pig enterobacteriaceae harmful bacteria growth, improve
Its intestinal health, and then realize the effect of prevention and treatment diarrhea.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
In the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of by anyone skilled in the art,
It should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of the claim
Subject to enclosing.
SEQUENCE LISTING
<110>Beijing middle peasant Hong Ke Bioisystech Co., Ltd
<120>one Accharomyces cerevisiae bacterium HKB-36 and its application
<130> 20180705
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 554
<212> DNA
<213>unknown
<400> 1
aaaagctcaa atttgaaatc tggtaccttc ggtgcccgag ttgtaatttg gagagggcaa 60
ctttggggcc gttccttgtc tatgttcctt ggaacaggac gtcatagagg gtgagaatcc 120
cgtgtggcga ggagtgcggt tctttgtaaa gtgccttcga agagtcgagt tgtttgggaa 180
tgcagctcta agtgggtggt aaattccatc taaagctaaa tattggcgag agaccgatag 240
cgaacaagta cagtgatgga aagatgaaaa gaactttgaa aagagagtga aaaagtacgt 300
gaaattgttg aaagggaagg gcatttgatc agacatggtg ttttgtgccc tctgctcctt 360
gtgggtaggg gaatctcgca tttcactggg ccagcatcag ttttggtggc aggataaatc 420
cataggaatg tagcttgcct cggtaagtat tatagcctgt gggaatactg ccagctggga 480
ctgaggactg cgacgtaagt caaggatgct ggcataatgg ttatatgccg cccgtcttga 540
accaccggac caaa 554
Claims (8)
1. a kind of antibacterial type yeast culture, which is characterized in that the yeast culture fermented by S. cervisiae HKB-36 and
At.
2. application of the yeast culture as described in claim 1 in terms of inhibiting bacterial growth.
3. application as claimed in claim 2, which is characterized in that
The bacterium includes Escherichia coli, staphylococcus aureus and salmonella.
4. a kind of preparation method of antibacterial type yeast culture as described in claim 1, which is characterized in that the preparation method
Including slant strains activation, seed liquor preparation, liquid aerobic fermentation and solid anaerobic fermentation step.
5. antibacterial type yeast culture preparation method as claimed in claim 4, which is characterized in that
In seed liquor preparation step, fluid nutrient medium includes that malt leaches powder 145g/L, chloramphenicol 0.08g/L, fluid nutrient medium
PH value is 5.8-6.8；Cultivation temperature is 28 DEG C -36 DEG C, revolving speed 120-200r/min, incubation time 18-30h.
6. antibacterial type yeast culture preparation method as claimed in claim 4, which is characterized in that
In liquid aerobic fermentation step, fluid nutrient medium includes that malt leaches powder 145g/L, chloramphenicol 0.08g/L, Liquid Culture
Base pH value is 5.8-6.8；Fermentation temperature is 28 DEG C -36 DEG C, fermentation time 24-36h, ventilatory capacity 1-4L/min.
7. the preparation method of antibacterial type yeast culture as claimed in claim 4, which is characterized in that
In solid anaerobic fermentation step, fermentation medium includes 2-4 parts of vinasse, 2-4 parts of maize peel, 1-3 parts of wheat bran and apple pomace
1-3 parts, culture medium carbon-nitrogen ratio is 2-7:1, moisture content 35%-70%；Fermentation temperature is 28 DEG C -36 DEG C, fermentation time 18-
48h。
8. antibacterial type yeast culture preparation method as claimed in claim 4, which is characterized in that the preparation method further includes
After solid anaerobic fermentation the step of preparing yeast culture finished product:
Fermentation temperature is adjusted to 60 DEG C -75 DEG C, broken wall is inactivated to the S. cervisiae HKB-36, drying is then crushed, is made
Yeast culture finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811120173.1A CN110150457B (en) | 2018-09-25 | 2018-09-25 | Bacteriostatic yeast culture and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811120173.1A CN110150457B (en) | 2018-09-25 | 2018-09-25 | Bacteriostatic yeast culture and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110150457A true CN110150457A (en) | 2019-08-23 |
| CN110150457B CN110150457B (en) | 2022-05-17 |
Family
ID=67645018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811120173.1A Active CN110150457B (en) | 2018-09-25 | 2018-09-25 | Bacteriostatic yeast culture and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110150457B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114829321A (en) * | 2019-12-17 | 2022-07-29 | 植物响应公司 | Methods and systems for reducing pathogens in organic materials |
| CN115678799A (en) * | 2022-09-07 | 2023-02-03 | 北京农学院 | Actinomycete culture and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693526A (en) * | 1996-03-29 | 1997-12-02 | Council Of Scientific & Industrial Research | Strains of yeast of saccharomyces cerevisiae and a process for the preparation of such strains of yeast |
| US20170037361A1 (en) * | 2014-04-23 | 2017-02-09 | Scandinavian Technology Group Ab | Saccharomyces cerevisiae strains |
| CN106434400A (en) * | 2016-08-30 | 2017-02-22 | 北京英惠尔生物技术有限公司 | Saccharomyces cerevisiae culture as well as preparation method and application of saccharomyces cerevisiae culture |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
-
2018
- 2018-09-25 CN CN201811120173.1A patent/CN110150457B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693526A (en) * | 1996-03-29 | 1997-12-02 | Council Of Scientific & Industrial Research | Strains of yeast of saccharomyces cerevisiae and a process for the preparation of such strains of yeast |
| US20170037361A1 (en) * | 2014-04-23 | 2017-02-09 | Scandinavian Technology Group Ab | Saccharomyces cerevisiae strains |
| CN106434400A (en) * | 2016-08-30 | 2017-02-22 | 北京英惠尔生物技术有限公司 | Saccharomyces cerevisiae culture as well as preparation method and application of saccharomyces cerevisiae culture |
| CN108085261A (en) * | 2017-12-29 | 2018-05-29 | 深圳市善成生物技术有限公司 | One Accharomyces cerevisiae and its culture and the application in feed |
Non-Patent Citations (2)
| Title |
|---|
| 刘龙海等: "奶牛饲料中酿酒酵母菌的分离鉴定及其发酵液的体外抑菌活性研究", 《中国畜牧兽医》 * |
| 高绪娜等: "酵母培养物高活性菌株的分离及发酵条件优化", 《中国饲料》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114829321A (en) * | 2019-12-17 | 2022-07-29 | 植物响应公司 | Methods and systems for reducing pathogens in organic materials |
| EP4077243A4 (en) * | 2019-12-17 | 2024-01-17 | Plant Response Inc | Methods and systems for pathogen mitigation in organic materials |
| CN115678799A (en) * | 2022-09-07 | 2023-02-03 | 北京农学院 | Actinomycete culture and preparation method and application thereof |
| CN115678799B (en) * | 2022-09-07 | 2023-09-01 | 北京农学院 | Actinomycete culture and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110150457B (en) | 2022-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102823726B (en) | It is a kind of improve cottonseed meal content and can detoxification biofermentation method | |
| CN101869184B (en) | Microbial feed additive and preparation method thereof | |
| CN101463329A (en) | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN103468594B (en) | Candidautilis strain and application thereof | |
| CN105385612B (en) | Candida utili bacterium, its composition and application | |
| CN100405922C (en) | Method for producing thallus protein forage by using solid state fermentation of pomace and dreg or stalks with mixed aerobic bacteria liquor | |
| CN105028897B (en) | A kind of cordyceps culturing medium fermented feed and preparation method thereof | |
| CN104757267A (en) | Apple pomace microbial culture starter and method for producing biological feed by apple pomace microbial culture starter | |
| CN104106727A (en) | Complete fermented feed and preparing method thereof | |
| CN108208335A (en) | A kind of technique for improving cotton dregs Forage Nutritive Value using microbe fermentation method | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN107974421A (en) | A kind of lactobacillus acidophilus and its screening technique and application, a kind of microbial inoculum | |
| CN109022333A (en) | A kind of preparation method and applications of composite microbial fermentation bacteria agent | |
| CN103289907A (en) | A candida utilis protein product and applications thereof in breeding industry | |
| CN109912354A (en) | A method of biological organic fertilizer is prepared using microorganism hot fermentation agricultural waste gurry | |
| CN101407762B (en) | Microbial solid inocula, and preparation and use thereof | |
| CN105543131A (en) | Compound bacteria/cottonseed meal fermented feed and preparation method thereof | |
| CN111534459A (en) | Lactobacillus fermentum for high yield of amylase and application of lactobacillus fermentum in preparation of fermented feed | |
| CN106306426A (en) | Novel process for corn silage | |
| CN103535525B (en) | Production method of biological feed additive rich in amino acids and proteins | |
| CN107712284A (en) | A kind of feed fermentation agent | |
| CN110150457A (en) | A kind of antibacterial type yeast culture and its application | |
| CN110317753A (en) | A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application | |
| CN104782906A (en) | Fermented feed for aquaculture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |