CN107712284A - A kind of feed fermentation agent - Google Patents

A kind of feed fermentation agent Download PDF

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Publication number
CN107712284A
CN107712284A CN201711114697.5A CN201711114697A CN107712284A CN 107712284 A CN107712284 A CN 107712284A CN 201711114697 A CN201711114697 A CN 201711114697A CN 107712284 A CN107712284 A CN 107712284A
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CN
China
Prior art keywords
fermentation agent
feed fermentation
feed
bacterium
livestock
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711114697.5A
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Chinese (zh)
Inventor
黎宇毅
李灵洁
陆世吉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Tian Sha Beach Agricultural Investment Co Ltd
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Guangxi Tian Sha Beach Agricultural Investment Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to CN201711114697.5A priority Critical patent/CN107712284A/en
Publication of CN107712284A publication Critical patent/CN107712284A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/70Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry

Abstract

The invention discloses a kind of feed fermentation agent, it is made up of the raw material of following parts by weight:50 65% microbial strains, 15 25% complex enzyme formulation, 1 5% composite assistant, 5 15% carrier stone flour Paris white or zeolite powder, 5 15% maize cob meal;The microbial strains are made up of the bacterium powder mixing of the candida utili bacterium of mass ratio 20 25%, 20 25% Bacillus acidi lactici, 15 20% enterococcus faecalis, 6 16% Lactobacillus plantarums, 17 23% lactobacillus cellobiosas.Feed fermentation agent of the present invention improves digestibility of the livestock to feed, produces multiple beneficial biological metabolic product, improves livestock meat level, reduces the livestock incidence of disease, reduces plant's drug-fast bacteria, and reduce livestock aquaculture cost.Improve agroecological environment simultaneously, reduce environmental pollution, solve people and animals and strive grain contradiction, promote the development of livestock breeding industry, there is great social benefit and economic benefit.

Description

A kind of feed fermentation agent
Technical field
The invention belongs to livestock products cultural technique field, and in particular to a kind of feed fermentation agent.
Background technology
Substantial amounts of cellulose and non-is typically contained in current market sales of conventional feed or the conventional feed of autogamy Starch-polysaccharides class carbohydrate, and livestock is relatively low to the digestibility of this kind of material, conventional feed is typically with corn, beans The dregs of rice and wheat bran etc are feed made of raw material, and its cellulose carbohydrate content is higher, in order to improve livestock to conventional feeding The absorptivity of material, some is using addition cellulose-decomposing bacteria such as actinomycetes, but effect is not notable, and What is more in feed class In be added into antibiotic, digested and assimilated though can so improve livestock, or even improve its disease-resistant performance, abuse of antibiotics is to domestic animal Poultry and human consumption its meat, cause larger harm, people, animal are developed immunity to drugs to form vicious circle, and human body is caused Greatly infringement.
The content of the invention
To solve above-mentioned the shortcomings of the prior art, the present invention provides a kind of feed fermentation agent, completely without using antibiosis Element, have feed conversion rate high, fermentation starting is fast, and required total time of fermenting is short and can suppress to attack bacterium outside in fermentation The advantages of growth and field planting.
To achieve these goals, the present invention provides following technical scheme, a kind of feed fermentation agent, by following parts by weight Raw material forms:50-65% microbial strains, 15-25% complex enzyme formulation, 1-5% composite assistant, 5-15% carrier stone flour Paris white or zeolite powder, 5-15% maize cob meal;The microbial strains are by mass ratio 20-25% candida utili Bacterium, 20-25% Bacillus acidi lactici, 15-20% enterococcus faecalis, 6-16% Lactobacillus plantarums, 17-23% lactobacillus cellobiosas Bacterium powder mixing composition.
Further, the complex enzyme formulation is cellulase, zytase, mannonase galactosidase, grape Carbohydrate oxidase, protease, each component in complex enzyme formulation account for complex enzyme formulation gross mass ratio and are:Cellulase 20-25%, Zytase 15-22%, mannase 10-20%, galactosidase 10-20%, glucose oxidase 8-18%, protease 10- 18%。
Further, the concentration of the candida utili bacterium is 3.0 × 108-5.0×108CFU/mL, Lactobacillus plantarum Concentration is 1.0 × 109-5.0 ×109CFU/mL。
Further, the Chinese herbal medicine of the composition composite assistant is any in peppery Liao's grass, the Radix Astragali, Tea Saponin, yucconin Kind is several.
Further, the preparation method of a kind of feed fermentation agent, comprises the following steps:
(1)Candida utili ferments:Candida utili strain in exponential phase is inoculated into by 5% inoculum concentration and gone out In bacterium YPD Liquid Cultures, 25-28 DEG C of shaken cultivation 20-24h;
(2)Plant lactobacillus ferments:Lactobacillus plantarum strain in exponential phase is inoculated into by 10% inoculum concentration and is equipped with In the glass infusion bottle of 90%MRS sterilising mediums, 30-34 DEG C of quiescent culture 24-30h;
(3)Mixed culture fermentation agent:By fermentation raw material 50-65% microbial strains, 15-25% complex enzyme formulation, 1-5% it is compound Auxiliary agent, 5-15% carrier stone flour Paris white or zeolite powder, 5-15% maize cob meal carry out mixed fermentation, produce feed fermentation Agent.
In summary, the present invention has the advantages that as a result of such scheme:
1st, feed fermentation agent of the present invention improves digestibility of the livestock to feed, produces multiple beneficial biological metabolic product, carries High livestock meat is horizontal, reduces the livestock incidence of disease, reduces plant's drug-fast bacteria, and reduce livestock aquaculture cost.
2nd, feed fermentation agent of the present invention can realize making full use of for the agricultural resources such as stalk, improve agroecological environment, subtract Few environmental pollution, solve people and animals and strive grain contradiction, promote the development of livestock breeding industry, there is great social benefit to be imitated with economical Benefit.
3rd, feed digestion absorptivity is improved, reduce the aquaculture cost of raiser, the Activities of Some Plants feed due to fermentative degradation The cell membrane of raw material, promote the release of nutrition inside plant cell, promote animal digestion rate and the contact rate of nutriment, institute The digestibility of feed nutrition is improved, simultaneously as substantial amounts of forage protein and starchiness during the fermentation, quilt The enzyme of microorganism secretion, and enzyme present in leavening are decomposed, generation small-molecular peptides, amino acid and low sugar, disaccharide Deng, so as to improve animal digestion their efficiency, the energy that animal digestion divides thing to be consumed has been saved, so, and correspondingly Improve feed digests and assimilates harmony capacity usage ratio.
4th, fermentation generates multiple beneficial biological metabolic product:Generate substantial amounts of probiotics, organic acid, amino acid, small The functional materials such as peptide, low peptide, multidimensional, biological antibiotic element, fragrance matter, UGF, can effectively improve animal Non-specific immunity, improve the premunition of animal, completely dispense with antibiotic, alleviate the generation of germ drug-fast bacteria, alleviate by The multiple vicious circle of aquaculture disease caused by this.Medical expenses are reduced, and reduce aquaculture cost.
5th, it is horizontal to improve meat, using fermented feed hello pig, meat is generally good, and meat is close, aqueous few, meat Perfume (or spice), lean meat percentage is high, and the incidence of disease is low;Aquaculture cost reduces by 10%, and the incidence of disease reduces by more than 50%.
Embodiment
With reference to embodiment, the present invention is described further.
Embodiment 1
A kind of feed fermentation agent, is made up of the raw material of following parts by weight:61% microbial strains, 16% complex enzyme formulation, 5% Composite assistant, 10% carrier stone flour Paris white, 8% maize cob meal;The microbial strains are by the production of mass ratio 25% Protein candidiasis, 20% Bacillus acidi lactici, 19% enterococcus faecalis, 16% Lactobacillus plantarum, 20% lactobacillus cellobiosas Bacterium powder mixing composition.
Wherein, complex enzyme formulation is that cellulase, zytase, mannonase galactosidase, grape are glycoxidative Enzyme, protease, each component in complex enzyme formulation account for complex enzyme formulation gross mass ratio and are:Cellulase 25%, zytase 22%th, mannase 14%, galactosidase 16%, glucose oxidase 8%, protease 15%;
The concentration of candida utili bacterium is 3.0 × 108-5.0×108CFU/mL, Lactobacillus plantarum concentration are 1.0 × 109-5.0 ×109CFU/mL;
The Chinese herbal medicine of composition composite assistant is peppery Liao's grass, yucconin by 1:1 ratio composition.
The preparation method of above-mentioned feed fermentation agent, comprises the following steps:
(1)Candida utili ferments:Candida utili strain in exponential phase is inoculated into by 5% inoculum concentration and gone out In bacterium YPD Liquid Cultures, 25-28 DEG C of shaken cultivation 20-24h;
(2)Plant lactobacillus ferments:Lactobacillus plantarum strain in exponential phase is inoculated into by 10% inoculum concentration and is equipped with In the glass infusion bottle of 90%MRS sterilising mediums, 30-34 DEG C of quiescent culture 24-30h;
(3)Mixed culture fermentation agent:By fermentation raw material 50-65% microbial strains, 15-25% complex enzyme formulation, 1-5% it is compound Auxiliary agent, 5-15% carrier stone flour Paris white or zeolite powder, 5-15% maize cob meal carry out mixed fermentation, produce feed fermentation Agent.
Embodiment 2
A kind of feed fermentation agent, is made up of the raw material of following parts by weight:58% microbial strains, 18% complex enzyme formulation, 3% Composite assistant, 10% zeolite powder, 11% maize cob meal;The microbial strains are the Candida utilis ferment by mass ratio 22% Female bacterium, the bacterium powder mixing of 23% Bacillus acidi lactici, 19% enterococcus faecalis, 15% Lactobacillus plantarum, 21% lactobacillus cellobiosas Composition.
Wherein, complex enzyme formulation is that cellulase, zytase, mannonase galactosidase, grape are glycoxidative Enzyme, protease, each component in complex enzyme formulation account for complex enzyme formulation gross mass ratio and are:Cellulase 21%, zytase 20%th, mannase 17%, galactosidase 17%, glucose oxidase 11%, protease 14%;
The concentration of candida utili bacterium is 3.0 × 108-5.0×108CFU/mL, Lactobacillus plantarum concentration reach 1.0 × 109- 5.0 ×109CFU/mL;
The Chinese herbal medicine for forming composite assistant is the Radix Astragali, Tea Saponin by 1:1 ratio composition;
The preparation method of above-mentioned feed fermentation agent is consistent with embodiment 1.
Embodiment 3
A kind of feed fermentation agent, is made up of the raw material of following parts by weight:54% microbial strains, 23% complex enzyme formulation, 2% Composite assistant, 9% carrier stone flour Paris white, 11% maize cob meal;The microbial strains are by the production of mass ratio 21% Protein candidiasis, 22% Bacillus acidi lactici, 18% enterococcus faecalis, 16% Lactobacillus plantarum, 23% lactobacillus cellobiosas Bacterium powder mixing composition.
Wherein, complex enzyme formulation is that cellulase, zytase, mannonase galactosidase, grape are glycoxidative Enzyme, protease, each component in complex enzyme formulation account for complex enzyme formulation gross mass ratio and are:Cellulase 20%, zytase 22%th, mannase 14%, galactosidase 18%, glucose oxidase 12%, protease 14%;
The concentration of candida utili bacterium is 3.0 × 108-5.0×108CFU/mL, Lactobacillus plantarum concentration reach 1.0 × 109- 5.0 ×109CFU/mL;
The Chinese herbal medicine for forming composite assistant is peppery Liao's grass, the Radix Astragali, Tea Saponin, yucconin by 1:1:1:1 ratio composition.
The preparation method of above-mentioned feed fermentation agent is consistent with embodiment 1.
The preferred embodiment of invention is the foregoing is only, is not intended to limit the invention, all spirit in the present invention Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.

Claims (5)

1. a kind of feed fermentation agent, it is characterised in that the feed fermentation agent is made up of the raw material of following parts by weight:50-65%'s Microbial strains, 15-25% complex enzyme formulation, 1-5% composite assistant, 5-15% carrier stone flour Paris white or zeolite powder, 5-15% maize cob meal;The microbial strains are candida utili bacterium by mass ratio 20-25%, 20-25% lactic acid bar Bacterium, 15-20% enterococcus faecalis, 6-16% Lactobacillus plantarums, 17-23% lactobacillus cellobiosas bacterium powder mixing composition.
A kind of 2. feed fermentation agent according to claim 1, it is characterised in that:The complex enzyme formulation be cellulase, Zytase, mannonase galactosidase, glucose oxidase, protease, each component in complex enzyme formulation account for compound Enzyme preparation gross mass ratio is:Cellulase 20-25%, zytase 15-22%, mannase 10-20%, galactosidase 10-20%, glucose oxidase 8-18%, protease 10-18%.
A kind of 3. feed fermentation agent according to claim 1, it is characterised in that:The concentration of the candida utili bacterium is 3.0×108-5.0×108CFU/mL, Lactobacillus plantarum concentration are 1.0 × 109-5.0 ×109CFU/mL。
A kind of 4. feed fermentation agent according to claim 1, it is characterised in that:It is described composition composite assistant Chinese herbal medicine be It is any one or several in peppery Liao's grass, the Radix Astragali, Tea Saponin, yucconin.
5. the preparation method of a kind of feed fermentation agent according to claim 1, it is characterised in that comprise the following steps:
(1)Candida utili ferments:Candida utili strain in exponential phase is inoculated into by 5% inoculum concentration and gone out In bacterium YPD Liquid Cultures, 25-28 DEG C of shaken cultivation 20-24h;
(2)Plant lactobacillus ferments:Lactobacillus plantarum strain in exponential phase is inoculated into by 10% inoculum concentration and is equipped with In the glass infusion bottle of 90%MRS sterilising mediums, 30-34 DEG C of quiescent culture 24-30h;
(3)Mixed culture fermentation agent:By fermentation raw material 50-65% microbial strains, 15-25% complex enzyme formulation, 1-5% it is compound Auxiliary agent, 5-15% carrier stone flour Paris white or zeolite powder, 5-15% maize cob meal carry out mixed fermentation, produce feed fermentation Agent.
CN201711114697.5A 2017-11-13 2017-11-13 A kind of feed fermentation agent Pending CN107712284A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110024910A (en) * 2019-05-17 2019-07-19 河南农业大学 The method for improving wormwood tunning quality with cellulase and composite probiotics ferment
CN113508875A (en) * 2021-07-21 2021-10-19 内蒙古珍牧饲料有限公司 Biological feed and preparation method thereof
CN114468123A (en) * 2022-03-16 2022-05-13 四川水利职业技术学院 Silage additive and preparation method and application thereof
CN114903118A (en) * 2022-05-17 2022-08-16 广西富鹏农牧有限公司 Feed leavening agent and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090085754A (en) * 2008-02-05 2009-08-10 대한민국(관리부서:농촌진흥청) Probiotic materials for livestock and preparing method thereof
CN102125163A (en) * 2010-12-31 2011-07-20 宜春强微生物科技有限公司 Feed ferment and method for producing fermented feed by utilizing same
CN104605167A (en) * 2013-11-04 2015-05-13 浙江金华神农农业科技有限公司 Feed fermentation agent and fermented feed
CN106834163A (en) * 2016-12-20 2017-06-13 湖南赛福资源饲料科技有限公司 Feed fermentation agent, fermented feed and preparation method thereof
CN106889306A (en) * 2017-02-27 2017-06-27 天津市农业生物技术研究中心 A kind of feed fermentation agent and the application on feed fermentation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090085754A (en) * 2008-02-05 2009-08-10 대한민국(관리부서:농촌진흥청) Probiotic materials for livestock and preparing method thereof
CN102125163A (en) * 2010-12-31 2011-07-20 宜春强微生物科技有限公司 Feed ferment and method for producing fermented feed by utilizing same
CN104605167A (en) * 2013-11-04 2015-05-13 浙江金华神农农业科技有限公司 Feed fermentation agent and fermented feed
CN106834163A (en) * 2016-12-20 2017-06-13 湖南赛福资源饲料科技有限公司 Feed fermentation agent, fermented feed and preparation method thereof
CN106889306A (en) * 2017-02-27 2017-06-27 天津市农业生物技术研究中心 A kind of feed fermentation agent and the application on feed fermentation

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110024910A (en) * 2019-05-17 2019-07-19 河南农业大学 The method for improving wormwood tunning quality with cellulase and composite probiotics ferment
CN113508875A (en) * 2021-07-21 2021-10-19 内蒙古珍牧饲料有限公司 Biological feed and preparation method thereof
CN114468123A (en) * 2022-03-16 2022-05-13 四川水利职业技术学院 Silage additive and preparation method and application thereof
CN114903118A (en) * 2022-05-17 2022-08-16 广西富鹏农牧有限公司 Feed leavening agent and application thereof

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