CN107568458A - A kind of immunological enhancement feed additive - Google Patents
A kind of immunological enhancement feed additive Download PDFInfo
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- CN107568458A CN107568458A CN201711001342.5A CN201711001342A CN107568458A CN 107568458 A CN107568458 A CN 107568458A CN 201711001342 A CN201711001342 A CN 201711001342A CN 107568458 A CN107568458 A CN 107568458A
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Abstract
The invention discloses a kind of immunological enhancement feed additive, belongs to agriculture field of feed additive technology, includes the raw material of following parts by weight：50 55 parts of gynostemma pentaphylla, 30 40 parts of perilla leaf, 1.2 1.6 parts of bacillus subtilis, 0.8 1.2 parts of saccharomyces cerevisiae, 0.02 0.04 parts of neotame, 8 12 parts of complex enzyme；Feed additive production technique of the present invention is simple, and use cost is low, can improve immunocyte in livestock and poultry body, immune protein, immune factor content, improves the general level of the health of animal body, reaches few medication, not medication, increases the purpose of culture benefit.
Description
Technical field
The invention belongs to agriculture field of feed additive technology, and in particular to a kind of immunological enhancement feed additive.
Background technology
Immune is a kind of efficiently conservative mechanism that multicellular animals is used for identifying and resisting external source courses of infection, right
Played an important role in anti-inflammatory and infection.Biologic artifact is lived in the environment full of pathogenic microorganism, passes through feed, skin
Contact etc., it is likely at any time by its infection so that morbidity.If the first line of defence of skin and mucous membrane composition is destroyed, host
The cause of disease of intrusion is removed with regard to starting congenital immunity and specific immunity, and courses of infection can be made a response rapidly congenital exempts from
Epidemic disease is the important defense mechanism of organism.
In recent years, cultivation industry disease becomes increasingly complex, and animal-breeding has high-volume, large-scale feature, but advises
It is that cultivation density is too high that what mould increase was brought, which directly affects, and growth of animal environment is poor, is particularly easy to illness, and the infection of disease
Rate is very high, and the autoimmune level of animal is more and more lower.The formula raising of scale chemical plant, cultivation density is very big, and high productivity can wait
Stress, cultivated animals is in low immune level and sub-health state, cause animal high incidence and high mortality.Supported to vast
Grow family and bring substantial amounts of economic loss, have a strong impact on culture benefit.
In traditional pest control method, antibiotic and chemicals are largely used as Main Means, result in
Pathogen develops immunity to drugs, and therapeutic effect declines；Simultaneously as the residual of medicine, the health and ring of consumer groups are had a strong impact on
Border safety.So many plants or feed factory change thinking by adding the materials such as traditional Chinese medicine powder or extract, probiotics, polysaccharide
It is expected to reach the health for improving animal.But the cost that these components have is higher, some effects are undesirable, and can not be from essence
It is upper to solve the problems, such as.
A kind of feed addictives for improving immunity of publication No. CN 105941850A are made up of one group of Chinese medicine composition, are sent out
After bright feed addictive mixes food, the incidence of disease of animal can be reduced, improves letting animals feed immunity, but used Chinese medicine
Composition species is more, and cost is also higher；A kind of immunologic pattern fleshy duck fodder additives of the A of publication No. CN 105767489 are by scaly holly root
10-20 parts, honeysuckle 10-20 parts, Radix Astragali 10-20 parts, radix glycyrrhizae 6-10 parts, motherwort 10-20 parts, Semen Cuscutae 10-20
Part, balloonflower root 8-10 parts, coix seed 4-6 parts, cape jasmine 5-10 parts, enzyme preparation 10-20 parts, probiotics 4-8 parts.The hair
Bright purpose is to provide a kind of safe and non-toxic immunologic pattern fleshy duck fodder additive.Using and Chinese medicine composition and enzyme system
Agent, the problem of same be present.
Therefore selection has no drug resistance, resourceful Chinese medicine, the probiotics of low cost and efficient enzyme preparation three one
Body acts synergistically, and improves animal health.This is that a kind is cheap, efficient compound formulation.This combination not only increases
Add Chinese medicine utilization rate, also from multi-faceted, Mutiple Targets establish complex immunity barrier jointly, lift animal body immunocompetence, and
And have and directly sterilize, it is antibacterial and other effects.
The content of the invention
A kind of overcome the deficiencies in the prior art of the present invention, there is provided immunological enhancement feed additive.By gynostemma pentaphylla, purple perilla
Leaf, bacillus subtilis, saccharomyces cerevisiae, complex enzyme, neotame mix.The feed addictive easily makes, and use cost is low,
Immunocyte in body can be improved, immune protein, immune factor content, the general level of the health of animal body is improved, reaches few medication,
Not medication, increase the purpose of culture benefit.
The technical solution adopted in the present invention is：A kind of immunological enhancement feed additive, include the original of following parts by weight
Material：Gynostemma pentaphylla 50-55 parts, perilla leaf 30-40 parts, bacillus subtilis 1.2-1.6 parts, saccharomyces cerevisiae 0.8-1.2 parts, neotame
0.02-0.04 parts, complex enzyme 8-12 parts.
Preferably include the raw material of following parts by weight：53 parts of gynostemma pentaphylla, 38 parts of perilla leaf, 1.4 parts of bacillus subtilis,
1 part of saccharomyces cerevisiae, 0.03 part of neotame, 10 parts of complex enzyme.
The CFU/g of described bacillus subtilis viable count >=150,000,000,000；The CFU/g of saccharomyces cerevisiae viable count >=20,000,000,000.
Described complex enzyme is to be by mass ratio：Cellulase：Zytase：Mannase：Pectase=3:1:2:1
Composition；The enzyme activity of the enzyme is the U/g of cellulase >=10,000, the U/g of zytase >=100,000, the U/g of mannase >=50,000, pectin
The U/g of enzyme >=100,000.
The preparation method of described immunological enhancement feed additive comprises the following steps.
a）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then co-grinding cross 40 mesh sieves.
b）By bacillus subtilis, saccharomyces cerevisiae, neotame is pre-mixed 10 minutes, then by 3:1:2:1 ratio is by cellulose
Enzyme, zytase, mannase, pectase mix 10 minutes after adding.
c）By a）And b）After gained semi-finished product mix 5 minutes, vacuum packaging is finished product.
Described feed addictive is directly made an addition in finishing pigs diets；Its addition is finishing pigs diets gross weight
0.3-0.5%。
Gynostemma pentaphylla：Gynostemma pentaphylla is Curcurbitaceae gynostemma pentaphyllum genus herbaceous perennial vine plant, the leaf courage of name seven, alias Herba Gynostemmatis,
Herba Gynostemmatis, Pentapanax leschenaultii, the entitled Herb Gynostemmae Pentaphylli of Japan etc..Gynostemma pentaphylla has strengthening spleen, tonifying kidney, preventing phlegm from forming and stopping coughing, antipyretic and antidote functions.Twist
Stock indigo plant is similar with ginseng all to contain protopanoxadiol saponin(e, and modern pharmacology research, which proves that it has, to be reduced blood fat, hypotensive, prevent
Only aging, suppression tumour, reduction blood glucose, strengthen immunity, sedation-analgesia, cardioprotection and liver, antiatherosclerosis, guarantor
Protect blood vessel etc. to act on, it also has obvious trophic nerve, protection cardiac muscle, improves memory, anti-aging, anti-oxidant, guarantor in addition
Liver etc. acts on.Many researchs show that addition gynostemma pentaphylla can lift the humoral immunity of pig in weanling pig feed and cell is exempted from
Epidemic disease function.
Perilla leaf：Purple perilla belongs to Labiatae purple perilla platymiscium, and it obstructs, leaf and fruit can be used as medicine.According to《Compendium of Materia Medica》Note
Carry, purple perilla has the promoting flow of qi and blood circulation, clearing heat and promoting diuresis, dissolving phlegm, sending down abnormally ascending, relievingd asthma, gas in mediation, beneficial the five internal organs, controls cholera, qi-restoratives consumptive disease, profit
The effect of intestines.Perilla leaf have ventilatings the lung and resolving phlegm, relieving exterior syndrome and dispelling cold, solving toxin of fish and crab, promoting the circulation of qi and in, it is antiabortive the effect of.Purple perilla also has
Very high value, it is edible, medicine is dual-purpose, have it is antitumor, antimicrobial, reduce the effect of blood glucose, removing toxic substances.Research shows,
Purple perilla, which has, improves laying hen blood Archon ratio, antibody titer.It is huge that pole significantly improves mouse weight, Thymus and spleen index, abdominal cavity
Phagocyte activity, content.Fructus Perillae quintessence oil has humidification to immune function of mice, can stimulate IL-2 and Ig M generation and release
Put, and a certain amount effect relation be present.
Illustrate that purple perilla has the effect of improving animal immune level.
Bacillus subtilis, saccharomyces cerevisiae：Enteron aisle oxygen is consumed, suppresses harmful bacteria growing, anaerobism ring is created for beneficial bacterium
Border；A variety of digestive ferments and metabolin are produced, are promoted beneficial to bacteria growing；Antibacterial peptide is produced, effectively sterilizes, is antibacterial.
Neotame：Sweet taste can cover taste uncomfortable in feed, improve feed palatability；Also can the digestion of induction port solinocrine
Enzyme, promote the digestion of feed.
Cellulase：Cellulase is the general name of a kind of compound enzyme system, can be by the cellulose degradation in feed into can digest
The reduced sugar of absorption, improve the nutritive value of feed.String can be also decomposed into glucose, it is more to eliminate non-starch in feed
The anti-oxidant action of sugar, and reduce the viscosity of intestinal contents.The main component of Chinese medicine cell membrane is cellulose, utilizes fiber
Plain enzyme can destroy the cell membrane of autonomic drug, promote the dissolution of active ingredient in autonomic drug, so as to strengthen the drug effect of autonomic drug.
Zytase：Zytase can crush plant cell wall, improve plant recovery of nutrient.Zytase is added in feed
It can be the relatively low small molecule segment of the degree of polymerization the higher xylan degrading of the degree of polymerization, the structure of cell membrane be destroyed, by cell
The nutriment of wall constraint discharges, and the digestive ferment in animal alimentary canal fully contacts, so as to improve disappearing for various nutrients
Rate.Similarly, the utilization rate of Chinese medicine can also be increased.Zytase can reduce chyme viscosity, improve Apparent metabolizable energy, thorn
Swash endogenous digestion enzyme secretion, improve endogenous digestive enzyme activity, external source digestive ferment has with endogenous digestive ferment action effect is superimposed effect
Should.Zytase can reduce microorganism species total amount, regulation intestinal microflora balance.
Mannase：Mannosan enzyme hydrolysis mannocarolose, the manna oligosacchride of acquisition have good biological regulation work(
Can, such as：Promote the growth of beneficial bacteria of intestinal tract, mitigate constipation, promote absorption of nutrient ingredients, improve fodder energy utilization rate, improve
Feed conversion rate, reduce feed weightening ratio and improve breeding performonce fo animals etc..'beta '-mannase can help to break cell
The compact texture of wall, discharges nutriment, is fully contacted with digestive ferment, so as to improve the digestive utilization ratio of nutrient.Can be special
Property beta-mannase is degraded to the oligosaccharide such as mannose, reduces soluble mannan viscosity, retentiveness, reduce and water
The interaction of molecule, hence it is evident that reduce intestines and stomach chyme viscosity, promote the digestibility and utilization of nutriment, improve the nutrition of feed
Value.
Pectase：Pectin is to be present in plant cell wall and intercellular one kind is adhered material, it be galacturonic acid with
α-Isosorbide-5-Nitrae key connection.In the plant cell wall of maturation, cellulose, hemicellulose, lignin and pectin are combined closely one
Rise, thus greatly reduce digestibility of the animal to cell wall substance, and lack in animal intestinal tract and decompose lignin and pectin
Enzyme, simultaneously because pectin to cellulose, hemicellulose parcel and be adhered effect, also counteracts that some enzymes to cellulose and half
The effect of fiber, only in the presence of pectin decomposing enzyme, cellulose and hemicellulose can be just discharged after decomposing pectin.Pectin
The one kind of enzyme as non-starch polysaccharide enzyme, there is many critical functions, such as degrading plant cell membrane, eliminates ANFs, improves
Function of intestinal canal, improve endogenous enzyme activity etc..Pectase is the general name for a variety of enzymes that can be catalyzed pectic substance decomposition, can be effectively
Crack β-Isosorbide-5-Nitrae-glycosidic bond, slough hydrone, decompose the pectic substance between plant cell wall, destroy cell tissue, release nutrition into
Divide and utilized by animal.
Compared with prior art the invention has the advantages that：
The present invention is mixed by gynostemma pentaphylla, perilla leaf, bacillus subtilis, saccharomyces cerevisiae, complex enzyme, neotame.The feed adds
Agent is added easily to make, use cost is low, can improve immunocyte in body, immune protein, immune factor content, improve animal machine
The general level of the health of body, reaches few medication, not medication, increases the purpose of culture benefit.
Embodiment
Technical scheme is described in detail with reference to embodiment, but protection domain is not limited by this.
Embodiment 1
Weigh following raw material：Gynostemma pentaphylla 50kg, perilla leaf 30kg, bacillus subtilis 1.2kg, saccharomyces cerevisiae 0.8kg, neotame
0.02kg, complex enzyme 8kg.
Specific preparation process：1）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then crushed 40 mesh sieves.2）First by withered grass
Bacillus, saccharomyces cerevisiae, neotame are pre-mixed 10 minutes.Then it is 3 in mass ratio:1:2:1 ratio is by cellulase, xylan
Enzyme, mannase, pectase mix 10 minutes after adding.3）By 1）With 2）After gained semi-finished product mix 5 minutes, vacuum packaging
As finished product.
The finished product feedstuff additive prepared is directly made an addition in finishing pigs diets；Its addition is finishing pigs diets gross weight
The 0.3% of amount.
Embodiment 2
Weigh following raw material：Gynostemma pentaphylla 55kg, purple perilla 40kg, bacillus subtilis 1.6kg, saccharomyces cerevisiae 1.2kg, neotame
0.04kg, complex enzyme 12kg.
Specific preparation process：1）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then crushed 40 mesh sieves.2）First by withered grass
Bacillus, saccharomyces cerevisiae, neotame are pre-mixed 10 minutes.Then it is 3 in mass ratio:1:2:1 ratio is by cellulase, xylan
Enzyme, mannase, pectase mix 10 minutes after adding.3）By 1）With 2）After gained semi-finished product mix 5 minutes, vacuum packaging
As finished product.
The finished product feedstuff additive prepared is directly made an addition in finishing pigs diets；Its addition is finishing pigs diets gross weight
The 0.5% of amount.
Embodiment 3
Weigh following raw material：Gynostemma pentaphylla 52.5kg, perilla leaf 35kg, bacillus subtilis 1.4kg, saccharomyces cerevisiae 1kg, neotame
0.03kg, complex enzyme 10kg.
Specific preparation process：1）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then crushed 40 mesh sieves.2）First by withered grass
Bacillus, saccharomyces cerevisiae, neotame are pre-mixed 10 minutes in proportion.Then it is 3 in mass ratio:1:2:1 ratio by cellulase,
Zytase, mannase, pectase mix 10 minutes after adding.3）By 1）With 2）After gained semi-finished product mix 5 minutes, very
Empty package is finished product.
The finished product feedstuff additive prepared is directly made an addition in finishing pigs diets；Its addition is finishing pigs diets gross weight
The 0.4% of amount.
Embodiment 4
Weigh following raw material：Gynostemma pentaphylla 53kg, perilla leaf 38kg, bacillus subtilis 1.4kg, saccharomyces cerevisiae 1kg, neotame
0.03kg, complex enzyme 10kg.
Specific preparation process：1）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then crushed 40 mesh sieves.2）First by withered grass
Bacillus, saccharomyces cerevisiae, neotame are pre-mixed 10 minutes.Then it is 3 in mass ratio:1:2:1 ratio is by cellulase, xylan
Enzyme, mannase, pectase mix 10 minutes after adding.3）By 1）With 2）After gained semi-finished product mix 5 minutes, vacuum packaging
As finished product.
The finished product feedstuff additive prepared is directly made an addition in finishing pigs diets；Its addition is finishing pigs diets gross weight
The 0.4% of amount.
Feeding effect is tested.
Experiment prepares：Test and carry out feeding experiment on Ruicheng County pig farm in April, 2017.96 body weight 80 of selection ±
5kg, healthy DLY tri-crossbreeding are randomly divided into control group, totally 4 groups of experiment 1,2,3, and every group of 3 repetitions are each to repeat
8 pigs.Control group fed corn bean pulp type basal diet, experiment 1,2,3 group respectively in basal diet addition 0.3%, 0.4%,
0.5% additive, test period 30 days.
Experiment process：Effective feeding time is 30 days, and pig feeds according to plant's feeding program, freely drunk during experiment
Water, on-test, claim every circle pig just starting weight, the pig state of mind, incidence are recorded during experiment.It is each on the day of off-test
2 pigs are selected in repetition, and 2 parts of blood is gathered in the vena cava anterior of every pig with vacuum blood collection tube, and 1 part with conventional vacuum heparin tube
Take a blood sample 5 mL, and 3 500 r/min centrifuges 5 min, separates serum, -20 DEG C of refrigerators freeze, for determining Serum Indexes；1
Part uses the mL of EDTAP dipotassium ethylene diamine tetraacetate (EDTA-K2) anti-freezing blood sampling tube 5, is detected for normal blood index.
Result of the test：In daily ration add 0.3%, 0.4%, 0.5% additive significantly improve total serum protein, globulin,
Leucocyte, lymphocyte, intermediate cell, granulocyte, intermediate cell content.But MPW, Platelet large cell ratio have
Declined.Immunoglobulin G content is improved in addition, reduces TNFa content.Wherein the Total albumen content point
11.77%, 9.81%, 11.98% is not improved；Globulin content significantly improves 26.23%, 28.26%, 24.87%；Leucocyte shows
Work improves 53.10%, 47.16%, 49.32%；Lymphocyte significantly improves 35.68%, 30.78%, 33.90%；Intermediate cell
Significantly improve 50.68%, 47.58%, 43.55%；Granulocyte significantly improves 65.98%, 60.82%, 52.58%；Intermediate cell
Percentage significantly improves 41.51%, 39.06%, 37.46%；MPW reduces 7.51%, 7.58%, 7.39%；Blood
Platelet ratio increases 15.52%, 16.14%, 17.15%；TNFa reduces 20.67%, 16.29%, 17.01%；Exempt from
Epidemic disease Protein G increases 10.02%, 11.26%, 8.09%.
Conclusion (of pressure testing)：This additive of addition 0.3-0.5% improves Protein retention in daily ration, while improves and exempt from
Epidemic disease albumen and immune factor content, so as to improve animal anti-inflammatory, resisting stress, resistance against diseases.
Experiment detailed results see the table below.
Influence of the immunological enhancement feed additive of table 1 to growing and fattening pigs blood parameters.
Influence of the immunological enhancement feed additive of table 2 to growing and fattening pigs routine blood indexes.
Influence of the immunological enhancement feed additive of table 3 to growing and fattening pigs sero-immunity index.
Above content is to combine the further description that specific preferred embodiment is done to the present invention, it is impossible to is assert
The embodiment of the present invention is only limitted to this, for general technical staff of the technical field of the invention, is not taking off
On the premise of from the present invention, some simple deduction or replace can also be made, should all be considered as belonging to the present invention by being submitted
Claims determine scope of patent protection.
Claims (6)
1. a kind of immunological enhancement feed additive, it is characterised in that include the raw material of following parts by weight：Gynostemma pentaphylla 50-55 parts,
Perilla leaf 30-40 parts, bacillus subtilis 1.2-1.6 parts, saccharomyces cerevisiae 0.8-1.2 parts, neotame 0.02-0.04 parts, complex enzyme
8-12 parts.
2. a kind of immunological enhancement feed additive according to claim 1, it is characterised in that including following parts by weight
Raw material：53 parts of gynostemma pentaphylla, 38 parts of perilla leaf, 1.4 parts of bacillus subtilis, 1 part of saccharomyces cerevisiae, 0.03 part of neotame, complex enzyme 10
Part.
A kind of 3. immunological enhancement feed additive according to claim 1 or 2, it is characterised in that described withered grass gemma
The CFU/g of bacillus viable count >=150,000,000,000；The CFU/g of saccharomyces cerevisiae viable count >=20,000,000,000.
4. a kind of immunological enhancement feed additive according to claim 1 or 2, it is characterised in that described complex enzyme is
It is by mass ratio：Cellulase：Zytase：Mannase：Pectase=3:1:2:1 composition；The enzyme activity of the enzyme is fiber
The U/g of plain enzyme >=10,000, the U/g of zytase >=100,000, the U/g of mannase >=50,000, the U/g of pectase >=100,000.
A kind of 5. immunological enhancement feed additive according to claim 1 or 2, it is characterised in that described Immune enhancement
The preparation method of feed addictive comprises the following steps：
a）Gynostemma pentaphyllum Makino and the selected removal of impurities of perilla leaf, then co-grinding cross 40 mesh sieves；
b）By bacillus subtilis, saccharomyces cerevisiae, neotame is pre-mixed 10 minutes, then by 3:1:2:1 ratio is by cellulase, wood
Dextranase, mannase, pectase mix 10 minutes after adding；
c）By a）And b）After gained semi-finished product mix 5 minutes, vacuum packaging is finished product.
A kind of 6. immunological enhancement feed additive according to claim 1 or 2, it is characterised in that described feed addition
Agent is directly made an addition in finishing pigs diets；Its addition is the 0.3-0.5% of finishing pigs diets gross weight.
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Application publication date: 20180112 |