CN104206786A - Traditional Chinese medicine microecological preparation for meat chicken, preparation method and applications thereof - Google Patents
Traditional Chinese medicine microecological preparation for meat chicken, preparation method and applications thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine microecological preparation for meat chicken, a preparation method and applications thereof, and belongs to the technical fields of veterinary traditional Chinese medicine and feed additive. The traditional Chinese medicine microecological preparation is mainly composed of the following traditional Chinese herbals in parts by weight: 2 to 10 parts of radix codonopsis, 2 to 10 parts of radix astragali, 2 to 8 parts of poria cocos, 1 to 5 parts of medicated leaven, 1 to 6 parts of malt, 1 to 5 parts of haw, 1 to 3 parts of licorice, and 1 to 3 parts of betel nut; and also comprises the following components in parts by volume: 1 to 3 parts of lactobacillus acidophilus liquid, 1 to 6 parts of enterococcus faecalis liquid, and 1 to 8 parts of bacillus subtilis liquid; wherein the total weight ratio of the traditional Chinese herbals to bacterium liquids is (1-5):(45-55). Through compounding eight pure natural traditional Chinese herbals mentioned above and three beneficial bacteria, the preparation can generate a plurality of hydrolytic enzymes in animals, and the enzymes can promote the degradation of protein, starch, fat, and hemicelluloses, so the preparation can promote the digestion and absorption of nutrients in feed.
Description
Technical field
The present invention is specifically related to a kind of broiler chicken Chinese medicine microecological preparation, and the preparation method and application of this Chinese medicine microecological preparation, belongs to technical field of Chinese medicine for animals and field of feed additive technology.
Background technology
Along with developing rapidly of animal husbandry, antibiotic, chemical synthetic drug, hormone medicine as feed addictive all drawbacks gradually be familiar with by people.While killing harmful levels of pathogens, also kill the beneficial organism in enteron aisle as antibiotic, cause microorganism species in enteron aisle uneven.In addition, medicine residual in animal products can have a strong impact on treatment and the heredity of human diseases, directly affects the health of the mankind.Therefore, find bird feed additive that is safe and reliable, Be very effective and become problem demanding prompt solution.
The advantage of Chinese herbal medicine, diverse in function natural using it, safe and reliable, economic environmental protection in China as feed addictive historical origin for a long time.Chinese herbal feed additive, as the term suggests be take Chinese herbal medicine as the feed addictive that raw material is made, although some scholar is classified to non-nutritional feed additive, is also included into medicine class feed addictive by country's examination & approval and management.But, be also natural products because Chinese medicine is medicine, containing plurality of active ingredients, there are all effects of feed addictive substantially, can be used as an independently class feed addictive.With a long history as feed addictive of China's application Chinese medicine, just started for promoting growth of animal, weightening finish and disease preventing and treating before more than 2,000 years.
Summary of the invention
The object of this invention is to provide a kind of broiler chicken Chinese medicine microecological preparation.
Meanwhile, the present invention also provides a kind of preparation method of broiler chicken Chinese medicine microecological preparation.
Finally, the present invention also provides a kind of broiler chicken Chinese medicine microecological preparation as the application of feed addictive.
In order to realize above object, the technical solution adopted in the present invention is:
A kind of broiler chicken Chinese medicine microecological preparation, be prepared from primarily of following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 2 ~ 10 parts, the Radix Astragali 2 ~ 10 parts, 2 ~ 8 parts, Poria cocos, Medicated Leaven 1 ~ 5 part, 1 ~ 6 part, Fructus Hordei Germinatus, hawthorn 1 ~ 5 part, 1 ~ 3 part, Radix Glycyrrhizae, betel nut 1 ~ 3 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 1 ~ 3 part, enterococcus faecalis bacterium liquid 1 ~ 6 part, bacillus subtilis bacterium liquid 1 ~ 8 part, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 3:50(1 ~ 5): (45 ~ 55).
Preferably, a kind of broiler chicken Chinese medicine microecological preparation, be prepared from primarily of following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 4 parts, the Radix Astragali 4 parts, 4 parts, Poria cocos, Medicated Leaven 2 parts, 2 parts, Fructus Hordei Germinatus, hawthorn 2 parts, 1 part, Radix Glycyrrhizae, betel nut 1 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 2 parts, enterococcus faecalis bacterium liquid 3 parts, bacillus subtilis bacterium liquid 5 parts, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 3:50.
The concentration of described lactobacillus acidophilus bacterium liquid is 0.5 × 10
8~ 1.0 × 10
8cFU/ml.
The concentration of described enterococcus faecalis bacterium liquid is 0.5 × 10
8~ 1.0 × 10
8cFU/ml.
The concentration of described bacillus subtilis bacterium liquid is 0.5 × 10
8~ 1.0 × 10
8cFU/ml.
Each bulk drug effect is summarized as follows:
Radix Codonopsis: be the root of Campanulaceae Codonopsis plant Radix Codonopsis, element flower Radix Codonopsis, radix codonpsis tangshen, pipe flower Radix Codonopsis, C. subglobosa, C. canescens; Containing carbohydrate as fructose, synanthrin, polysaccharide and 4 kinds of heteroglycan CP
1, CP
2, CP
3, CP
4glycoside is as syringin, n-hexyl-β-glucopyranoside, ethyl-α-D-fructofuranoside, tangshenoside, alkaloid and nitrogen containing component, as choline, loline etc., also contain the various active compositions such as syringaldehyde, vanillic acid, pyromucic acid, atractyloide I, II; Radix Codonopsis nature and flavor are sweet, flat, return spleen, lung channel; Can strengthening the spleen and tonifying the lung, nourishing generate fluid; Cure mainly weakness of the spleen and the stomach, anorexia and loose stool, limbs fatigue, the deficiency syndrome of the lung are breathed with cough, spontaneous perspiration of breathing hard, all cards of qi-blood deficiency; Modern pharmacology prove Radix Codonopsis have strengthen body stress ability, enhanced machine body immunity function, delay senility, antiulcer, antitumor booster action etc.
The Radix Astragali: be the root of pulse family Astragalus astragalus mongolicus and Astragalus membranacus; Containing saponin component as astragalin, isoflavones components is as calycosin, and fatty acid is as palmitic acid and linoleic acid, and polysaccharide is as astragalus polyose, heteroglycan etc., and nearly 20 various trace elements; Radix Astragali nature and flavor are sweet, and temperature, returns lung, the spleen channel; Energy benefiting qi and raising yang, strengthening exterior and reducing sweat, inducing diuresis for removing edema, promoting pus discharge and tissue regeneration; Cure mainly the disease of all Qi and blood deficiencies, as splenasthenic diarrhea, cough due to deficiency of the lung, prolapse of the anus, metroptosis, spontaneous perspiration, night sweat, oedema, blood-arthralgia, ulcer difficulty is burst or is burst for a long time and do not hold back; Modern pharmacology proves that the Radix Astragali can affect organism metabolism and has antioxidation, can also antithrombotic, antiviral, Antiarthritic, anticancer, anti-inflammatory and antalgic, protection cardio-cerebrovascular and promote hematopoiesis function.
Poria cocos: for Polyporaceae hole of crouching belongs to the sclerotium of fungi Poria cocos; Containing triterpenes as pachymic acid, 16 Alpha-hydroxy eburicoic acids, β-para hydroxybenzene acyl group dehydroeburicoic acid etc., polysaccharide as the callose etc. of pachyman, pachymaran and height, branch, also containing the various active composition such as ergosterol, sad, hendecanoic acid, laurate, dodecenoic acid, palmitic acid, dodecenoic acid fat, sad fat and inorganic elements; Nature and flavor are sweet, light, flat, the thoughts of returning home, spleen, lung, kidney channel; Energy clearing damp and promoting diuresis, strengthening the spleen and stomach, antitoxic heart-soothing and sedative; Cure mainly difficult urination, oedema turgor, phlegm and retained fluid cough inverse, vomiting, insufficiency of the spleen food less, have loose bowels, palpitation and restlessness, insomnia forgetfulness etc.; Modern pharmacology proves that Poria cocos has diuresis, anticancer and immunological enhancement etc.
Medicated Leaven (Divine Comedy): for the medicines such as polygonum flaccidum, sweet wormwood, almond add after flour or wheat bran mixing through the herbal leaven made of fermenting; For yeast preparation, containing saccharomycete, amylase, vitamin B complex, ergosterol, protein, fat and volatilization wet goods; Nature and flavor sweet, acrid, warm, returns spleen, stomach warp; Energy promoting digestion and removing indigestion, strengthening the spleen and stomach; Cure mainly retention of food and drink, indigestion, abdominal fullness and distention, poor appetite, vomiting dysentery etc.; Modern pharmacology proves that Medicated Leaven has effects such as promoting digestive function, improve a poor appetite.
Fructus Hordei Germinatus: be the germination caryopsis of grass family Hordeum plant barley; The main alkaloids that contains is as hordenine, fructus hordei germinatus guanidine alkali A and B, candine, adenine, choline, protein, amino acid, Cobastab, D and E, cromoci etc.; Nature and flavor are sweet, flat, return spleen, stomach warp; Energy promoting digestion and removing indigestion, delaactation; Cure mainly dyspepsia, abdominal distention and diarrhea rush down, n and V, poor appetite, milk smoulder, swollen breasts etc.; Modern pharmacology prove Fructus Hordei Germinatus have hypoglycemic, anti-oxidant, promote the effect such as mammary gland secretion lactation.
Hawthorn: be the ripening fruits of rose family may large-fruited Chinese hawthorn, hawthorn; Containing flavonoids as left-handed epicatechin, acids is as chlorogenic acid and citric acid, and aliphatic acid is as the composition such as linoleic acid and palmitic acid; Hawthorn nature and flavor are sour, sweet, and tepor, returns spleen, stomach, Liver Channel; Can food digesting stomach fortifying, promoting the circulation of qi is fallen apart the stasis of blood; Cure mainly eating accumulation, abdominal distention, dysentery, dysmenorrhea due to blood stasis, through closing, postpartum abdominal pain, lochiorrhagia, hernia or testiclar gall, hyperlipemia etc.; Modern pharmacology proves that hawthorn has the effects such as short digestion, hypertension and hyperlipemia, anti-oxidant, anti-cancer, protection cardio-cerebrovascular and enhancing function of immune system.
Radix Glycyrrhizae: be the root and rhizome of pulse family glycyrrhiza genus Radix Glycyrrhizae, smooth bark Radix Glycyrrhizae, glycyrrhiza inflate bat; Containing triterpenoid saponin as glycyrrhizin, Glycyrrhiza Uralensis saponin A, B etc., flavone compound is as 4',7-Dihydroxyflavanone, liquiritin etc., coumarin kind compound is as glycycoumarin, glycyol etc., alkaloids is as quinolines, iloquinoline derivative etc., polysaccharide, as Radix Glycyrrhizae glucan GBW, licorice polysaccharide UA, UB, UC etc., also contains the plurality of active ingredients such as Radix Glycyrrhizae benzofuran, n-tricosane; Radix Glycyrrhizae nature and flavor are sweet, flat, return spleen, stomach, the heart, lung channel; Can and middle emergency, moistening lung, removing toxic substances, coordinating the drug actions of a prescription; Process with control weakness of the spleen and the stomach, burnout food less, stomachache loose stool, the anxious pain of four limbs contraction, palpitaition, hysteria, consumptive lung disease cough, raw with controlling abscess of throat, carbuncle pyogenic infections from tumour or sore, infant fetal toxin and medicine, food poisoning etc.; Modern pharmacology proves that Radix Glycyrrhizae has antimicrobial, anti-inflammatory, antibechic, eliminates the phlegm, antitumor, anti-sudden change, the effect such as anti-oxidant, to digestive system, cardiovascular system, reproductive system and brain, renal function etc., there is regulating action.
Betel nut: be the seed of Palmae Areca plant betel nut; Containing alkaloids as arecaline, arecaidine, guvacoline, demethyl arecaidine, arecolidine, homarecoline etc., tannin, as cianidanol, left-handed epicatechin, former Cyanidin A-1, B-1 and B-2 and betel nut tannin A, B two compounds, also contains aliphatic acid if laurate, myristic acid, palmitic acid, stearic acid, dextrorotation etc. and amino acids are as various active compositions such as proline, tryptophan, methionine, tyrosine; Nature and flavor are bitter, pungent, and temperature, returns stomach, large intestine channel; Energy expelling parasites and food tagnancy, the capable water of lower gas, preventing malaria; Cure mainly malnutrition due to parasitic infestation, eat stagnant, abdominal distention, rush down dysentery after heavily etc.; Modern pharmacology proves that betel nut has expelling parasite, resisting pathogenic microbes, the effects such as spiral shell of going out.
A kind of broiler chicken Chinese medicine microecological preparation, can adopt conventional method to prepare.The present invention only provides a kind of concrete preparation method, comprises the following steps:
(1) accurately get Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae and betel nut according to parts by weight, pulverize after oven dry, sieve, then boiling 3 times, merge 3 decocting liquids, filter liquid is for subsequent use;
(2) lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid and bacillus subtilis bacterium liquid is accurately got according to volume parts, according to the ratio between bacterium liquid total amount and bulk drug total amount after mixing, mixed bacteria liquid is joined in the liquid of step (1), anaerobic fermentation in confined conditions, tunning is through freeze drying and get final product.
Bake out temperature in described step (1) is 60 ~ 80 DEG C.
Sieving as crossing 60 ~ 100 mesh sieves in described step (1).
In described step (1), boiling is the water that each decoction adds 5 ~ 6 times amount, decocts 1 ~ 2 hour.
In described step (2), the preparation method of lactobacillus acidophilus bacterium liquid is: be first inoculated in slant medium by lactobacillus acidophilus, being joined by the suspension that slant medium produces in MRS fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
In described step (2), the preparation method of enterococcus faecalis bacterium liquid is: be first inoculated in slant medium by enterococcus faecalis, being joined by the suspension that slant medium produces in MRS fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
In described step (2), the preparation method of bacillus subtilis bacterium liquid is: be first inoculated in slant medium by bacillus subtilis, being joined by the suspension that slant medium produces in LB fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
In described step (2), the temperature of anaerobic fermentation is 30 ~ 37 DEG C, and fermentation time is 36 ~ 72 hours.
Broiler chicken Chinese medicine microecological preparation, as an application for feed addictive, comprises the following steps: by weight percentage, by broiler chicken Chinese medicine microecological preparation with 0.2 ~ 0.4% amount add in broiler fodder.
Beneficial effect of the present invention:
The present invention is under the guidance of the dialectical theoretical method of traditional Chinese veterinary medicine defensive-qi-nutrient-blood, filters out Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae, betel nut totally 8 taste pure natural Chinese herbal medicine, according to monarch principle composition of prescription.In side, Radix Codonopsis, Radix Astragali strengthening the spleen and replenishing qi are monarch; Be aided with Medicated Leaven, hawthorn, Fructus Hordei Germinatus disappear long-pending appetizing; Help with Poria cocos clearing damp and promoting diuresis, the bowl spares of stomach invigorating spleen, betel nut eliminating stagnated food to destroy intestinal worms; Make with Radix Glycyrrhizae coordinating the drug actions of a prescription.All medicines share and can strengthen immunity of organisms, promote body growth.
The present invention adopts lactobacillus acidophilus, enterococcus faecalis and bacillus subtilis to prepare probiotics, wherein lactobacillus acidophilus can adjust gut flora balance, suppress the propagation of enteron aisle undesirable microorganism, secretion antibiotin class material, as acidolin, bacillus acidophilus's element, lactein etc., produces antagonism to pathogenic entero becteria; Enterococcus faecalis is conducive to improving microecological balance in enteron aisle, and control animal intestinal flora fauna is disorderly, can also decomposing protein be little peptide, and synthesis B family vitamin, strengthens the activity of macrophage, promote the immune response of animal, improve antibody horizontal etc.; Bacillus subtilis can consume rapidly the free oxygen in environment, causes enteron aisle hypoxemia, promotes that useful anaerobic bacteria grows, and produces the organic acids such as lactic acid, reduces enteron aisle pH value, indirectly suppresses the growth of other pathogenic bacteria.Growing of stimulating animal immune organ, activates T, bone-marrow-derived lymphocyte, improves immunoglobulin (Ig) and antibody horizontal, strengthens cellular immunity and humoral immune function, improves herd immunity.Three kinds of probio couplings, by the competitive exclusion effect of animal alimentary canal biology, suppress pathogen breeding, stop pathogen to be adsorbed in intestines wall, promote that it excretes; Be supplemented with bacteria group, adjustment microbial population of animal intestinal tract microecological balance; Play nutrition digestion-aid effect, improve food conversion ratio, synthesis nutriment, promotes growth of animal; Also carrying out prevent disease by strengthening non-specific immune function, improving immunity of organisms; Suppress the generation of the toxicant such as ammonia and amine in enteron aisle, improve environmental sanitation, environment purification.
The present invention adopts pure natural Chinese herbal medicine, and bulk drug is easy to get, cheap, safely, without hazard residue, little to the toxic and side effect of livestock and poultry body, not easily develops immunity to drugs, and plays efficacy of drugs mainly through mass action, bidirectional modulation, combined effect, strengthening vital QI to eliminate pathogenic factors.Chinese herbal medicine plays the effect of disease therapy and prevention and health care in breeding process, it not only directly acts on bacterium and virus after entering body, the immune organ of body can also be acted on, help to improve resistance, induction body produces endogenous interferon, utilize the foreign substance removing function of body body, expulsion bacterium and virus.Chinese herbal medicine is cured the disease and is not only recovered, but also helps body recovery physique, discharges toxin, kills two birds with one stone.The Chinese medicine undergone microbial fermentation, traditional Chinese medicine ingredients wherein, through microbial conversion, becomes Small molecular by large molecule, rapid-action, drug effect is high, 100% absorbed by body; Sweat can decompose the toxicant of Chinese herbal medicine simultaneously, and Chinese medicine preparation is really had no side effect.
The present invention is by above-mentioned 8 taste pure natural Chinese herbal medicine and three kinds of probios are composite prepares probiotics, multiple hydrolase can be produced in animal body, promote the decomposition of albumen, starch, fat and hemicellulose, be conducive to digesting and assimilating of feed Middle nutrition material, nutriment and some unknown somatomedin participation metabolism can be synthesized simultaneously, promote growth of animal; Probiotics carries out keeping healthy and treating as feed addictive, can reach the effect of getting twice the result with half the effort.
The method that the present invention prepares probiotics is simple, easy to operate, easy to use, and cost is low, is suitable for large-scale production and applies.
Detailed description of the invention
Following embodiment is only described in further detail the present invention, but does not form any limitation of the invention.
Embodiment 1
Broiler chicken Chinese medicine microecological preparation in the present embodiment, be prepared from by following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 2 parts, the Radix Astragali 10 parts, 2 parts, Poria cocos, Medicated Leaven 5 parts, 1 part, Fructus Hordei Germinatus, hawthorn 5 parts, 1 part, Radix Glycyrrhizae, betel nut 3 parts, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 1 part, enterococcus faecalis bacterium liquid 6 parts, bacillus subtilis bacterium liquid 1 part, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 1:55.
The preparation method of broiler chicken Chinese medicine microecological preparation in the present embodiment, comprises the following steps:
(1) accurately get Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae and betel nut according to parts by weight, dry rear pulverizing, cross 60 mesh sieves for 60 DEG C, boiling 3 times again, each decoction adds the water of 5 times amount, decocts 1.5 hours, merge 3 decocting liquids, filter liquid is for subsequent use;
(2) lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid and bacillus subtilis bacterium liquid is accurately got according to volume parts, according to the ratio between bacterium liquid total amount and bulk drug total amount after mixing, mixed bacteria liquid is joined in the liquid of step (1), anaerobic fermentation in confined conditions, the temperature of fermentation is 30 DEG C, fermentation time is 72 hours, by tunning freeze drying and get final product.
In described step (2), the preparation method of lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid, bacillus subtilis bacterium liquid is: be first inoculated in respective slant medium respectively by lactobacillus acidophilus, enterococcus faecalis, enterococcus faecalis, the suspension that each slant medium produces is joined successively in MRS fluid nutrient medium, MRS fluid nutrient medium, LB fluid nutrient medium to expand and cultivate, be respectively 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
In the present embodiment, broiler chicken Chinese medicine microecological preparation is as the application of feed addictive, comprises the following steps: by weight percentage, by the broiler chicken Chinese medicine microecological preparation of above-mentioned preparation with 0.3% amount add in broiler fodder.
Embodiment 2
Broiler chicken Chinese medicine microecological preparation in the present embodiment, be prepared from by following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 4 parts, the Radix Astragali 4 parts, 4 parts, Poria cocos, Medicated Leaven 2 parts, 2 parts, Fructus Hordei Germinatus, hawthorn 2 parts, 1 part, Radix Glycyrrhizae, betel nut 1 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 2 parts, enterococcus faecalis bacterium liquid 3 parts, bacillus subtilis bacterium liquid 5 parts, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 3:50.
The preparation method of broiler chicken Chinese medicine microecological preparation in the present embodiment, comprises the following steps:
(1) accurately get Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae and betel nut according to parts by weight, dry rear pulverizing, cross 100 mesh sieves for 80 DEG C, boiling 3 times again, each decoction adds the water of 6 times amount, decocts 2 hours, merge 3 decocting liquids, filter liquid is for subsequent use;
(2) lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid and bacillus subtilis bacterium liquid is accurately got according to volume parts, according to the ratio between bacterium liquid total amount and bulk drug total amount after mixing, mixed bacteria liquid is joined in the liquid of step (1), anaerobic fermentation in confined conditions, the temperature of fermentation is 37 DEG C, fermentation time is 36 hours, by tunning freeze drying and get final product.
The preparation method of described lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid, bacillus subtilis bacterium liquid is the same.
In the present embodiment, broiler chicken Chinese medicine microecological preparation is as the application of feed addictive, comprises the following steps: by weight percentage, by the broiler chicken Chinese medicine microecological preparation of above-mentioned preparation with 0.3% amount add in broiler fodder.
Embodiment 3
Broiler chicken Chinese medicine microecological preparation in the present embodiment, be prepared from by following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 10 parts, the Radix Astragali 2 parts, 8 parts, Poria cocos, Medicated Leaven 1 part, 6 parts, Fructus Hordei Germinatus, hawthorn 1 part, 3 parts, Radix Glycyrrhizae, betel nut 1 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 3 parts, enterococcus faecalis bacterium liquid 1 part, bacillus subtilis bacterium liquid 8 parts, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 1:9.
The preparation method of broiler chicken Chinese medicine microecological preparation in the present embodiment, comprises the following steps:
(1) accurately get Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae and betel nut according to parts by weight, dry rear pulverizing, cross 80 mesh sieves for 70 DEG C, boiling 3 times again, each decoction adds the water of 5 times amount, decocts 1.5 hours, merge 3 decocting liquids, filter liquid is for subsequent use;
(2) lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid and bacillus subtilis bacterium liquid is accurately got according to volume parts, according to the ratio between bacterium liquid total amount and bulk drug total amount after mixing, mixed bacteria liquid is joined in the liquid of step (1), anaerobic fermentation in confined conditions, the temperature of fermentation is 35 DEG C, fermentation time is 50 hours, by tunning freeze drying and get final product.
The preparation method of described lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid, bacillus subtilis bacterium liquid is the same.
In the present embodiment, broiler chicken Chinese medicine microecological preparation is as the application of feed addictive, comprises the following steps: by weight percentage, by the broiler chicken Chinese medicine microecological preparation of above-mentioned preparation with 0.3% amount add in broiler fodder.
Comparative example 1
Broiler chicken Chinese medicine composition in this comparative example, is made up of the bulk drug of following parts by weight: Radix Codonopsis 4 parts, the Radix Astragali 4 parts, 4 parts, Poria cocos, Medicated Leaven 2 parts, 2 parts, Fructus Hordei Germinatus, hawthorn 2 parts, 1 part, Radix Glycyrrhizae, betel nut 1 part.Preparation method comprises the following steps: accurately get each taste bulk drug according to parts by weight, mixes, cross 60 mesh sieves and get final product after pulverizing.
In this comparative example, broiler chicken Chinese medicine composition is as the application of feed addictive, comprises the following steps: by weight percentage, by the pulvis of above-mentioned preparation with 0.3% amount add in broiler fodder.
Test example
Experimental animal: random selecting 150 plumage 1 Day-old Broiler Chickens from certain chicken farm, is divided into 5 groups at random, often organizes 30 plumages.
Test group: test group 1 ~ 3 feed successively embodiment 1 ~ 3 prepare broiler fodder, control group fed comparative example 1 prepare broiler fodder; Blank group is fed broiler feed (in broiler feed not containing any drug ingedient).
Test method: broiler fodder prepared by Example 1 ~ 3 and broiler feed, continuous 45 days timing quantitative feeding 1 Day-old Broiler Chickens, the initial weight of record broiler chicken and the last weight of test, statistics adding weight and feed-weight ratio, the results detailed in following table 1.
Broiler growth and feed-weight ratio in each processed group of table 1
Conclusion: as can be seen from Table 1, the adding weight of test group is about 20g/ plumage, and feed-weight ratio is 1.68 ~ 1.87; The adding weight of control group is about 18.61/ plumage, feed-weight ratio 1.98; The adding weight of blank group is about 17.10g/ plumage, feed-weight ratio 2.2.Statistical analysis shows, the adding weight of test group is significantly higher than control group and blank group (P < 0.01), and feed-weight ratio is significantly lower than control group and blank group (P < 0.01).
Result of the test shows, probiotics prepared by the present invention can significantly improve broiler chicken daily gain, reduces feed-weight ratio, has significant facilitation to broiler growth, is suitable for actual raising application.During whole medication, test group chicken group does not reveal any abnormalities, and shows that the toxic and side effect of probiotics prepared by the embodiment of the present invention is little.
Claims (9)
1. a broiler chicken Chinese medicine microecological preparation, it is characterized in that: be prepared from primarily of following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 2 ~ 10 parts, the Radix Astragali 2 ~ 10 parts, 2 ~ 8 parts, Poria cocos, Medicated Leaven 1 ~ 5 part, 1 ~ 6 part, Fructus Hordei Germinatus, hawthorn 1 ~ 5 part, 1 ~ 3 part, Radix Glycyrrhizae, betel nut 1 ~ 3 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 1 ~ 3 part, enterococcus faecalis bacterium liquid 1 ~ 6 part, bacillus subtilis bacterium liquid 1 ~ 8 part, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is (1 ~ 5): (45 ~ 55).
2. broiler chicken Chinese medicine microecological preparation according to claim 1, it is characterized in that: be prepared from primarily of following raw material: bulk drug is counted with parts by weight: Radix Codonopsis 4 parts, the Radix Astragali 4 parts, 4 parts, Poria cocos, Medicated Leaven 2 parts, 2 parts, Fructus Hordei Germinatus, hawthorn 2 parts, 1 part, Radix Glycyrrhizae, betel nut 1 part, bacterium liquid is counted with volume fraction: lactobacillus acidophilus bacterium liquid 2 parts, enterococcus faecalis bacterium liquid 3 parts, bacillus subtilis bacterium liquid 5 parts, and the ratio of the gross weight of bacterium liquid and the gross weight of bulk drug is 3:50.
3. a preparation method for broiler chicken Chinese medicine microecological preparation as claimed in claim 1 or 2, is characterized in that: comprise the following steps:
(1) accurately get Radix Codonopsis, the Radix Astragali, Poria cocos, Medicated Leaven, Fructus Hordei Germinatus, hawthorn, Radix Glycyrrhizae and betel nut according to parts by weight, pulverize after oven dry, sieve, then boiling 3 times, merge 3 decocting liquids, filter liquid is for subsequent use;
(2) lactobacillus acidophilus bacterium liquid, enterococcus faecalis bacterium liquid and bacillus subtilis bacterium liquid is accurately got according to volume parts, according to the ratio between bacterium liquid total amount and bulk drug total amount after mixing, mixed bacteria liquid is joined in the liquid of step (1), anaerobic fermentation in confined conditions, tunning is through freeze drying and get final product.
4. the preparation method of broiler chicken Chinese medicine microecological preparation according to claim 3, is characterized in that: in described step (1), boiling is the water that each decoction adds 5 ~ 6 times amount, decocts 1 ~ 2 hour.
5. the preparation method of broiler chicken Chinese medicine microecological preparation according to claim 3, it is characterized in that: in described step (2), the preparation method of lactobacillus acidophilus bacterium liquid is: be first inoculated in slant medium by lactobacillus acidophilus, being joined by the suspension that slant medium produces in MRS fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
6. the preparation method of broiler chicken Chinese medicine microecological preparation according to claim 3, it is characterized in that: in described step (2), the preparation method of enterococcus faecalis bacterium liquid is: be first inoculated in slant medium by enterococcus faecalis, being joined by the suspension that slant medium produces in MRS fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
7. the preparation method of broiler chicken Chinese medicine microecological preparation according to claim 3, it is characterized in that: in described step (2), the preparation method of bacillus subtilis bacterium liquid is: be first inoculated in slant medium by bacillus subtilis, being joined by the suspension that slant medium produces in LB fluid nutrient medium to expand and cultivate, is 0.5 × 10 to the concentration of bacterium liquid during exponential phase
8~ 1.0 × 10
8cFU/ml and get final product.
8. the preparation method of broiler chicken Chinese medicine microecological preparation according to claim 3, is characterized in that: in described step (2), the temperature of anaerobic fermentation is 30 ~ 37 DEG C, and fermentation time is 36 ~ 72 hours.
9. a broiler chicken Chinese medicine microecological preparation as claimed in claim 1 or 2 is as the application of feed addictive, it is characterized in that: comprise the following steps: by weight percentage, by broiler chicken Chinese medicine microecological preparation with 0.2 ~ 0.4% amount add in broiler fodder.
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Application publication date: 20141217 |