CN105384562A - Pleurotus geesteranus medium - Google Patents

Pleurotus geesteranus medium Download PDF

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Publication number
CN105384562A
CN105384562A CN201511026340.2A CN201511026340A CN105384562A CN 105384562 A CN105384562 A CN 105384562A CN 201511026340 A CN201511026340 A CN 201511026340A CN 105384562 A CN105384562 A CN 105384562A
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parts
pocket mushrooms
raw
chinese medicine
weight
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李荣轩
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Guangxi Yanggui Biotechnology Co Ltd
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Guangxi Yanggui Biotechnology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

Abstract

The invention relates to the technical field of pleurotus geesteranus culture, in particular to a pleurotus geesteranus medium which is prepared from the following raw materials in parts by weight: 36 to 43 parts of cottonseed hull, 6 to 12 parts of bagasse, 11 to 22 parts of rice bran, 8 to 18 parts of traditional Chinese medicine additive, 6 to 13 parts of bran, and 0.8 to 2.1 parts of lime. The pleurotus geesteranus medium provided by the invention can promote the growth of the pleurotus geesteranus, improve the yield, improve inoxidizability and cancer resistance of the pleurotus geesteranus, the content of amino acid of the cultured pleurotus geesteranus is remarkably improved, and the used raw materials are mostly natural raw materials, so the medium is environment-friendly, harmless, and low in cost.

Description

A kind of pocket mushrooms substratum
Technical field
The present invention relates to pocket mushrooms culture technique field, particularly a kind of pocket mushrooms substratum.
Background technology
Pocket mushrooms has another name called Pleurotus geesteranus, yellowish-whitely to pick up the ears, handle bucket mushroom and small mushroom etc., and be subordinate to Basidiomycetes, Agaricales, Pleurotaceae, pleurotus, its sporophore list is raw or grow thickly, and a little shape is beautiful, mushroom lid diameter 1 ~ 3cm, has light grey and oyster white 2 kinds.Mushroom handle is sturdy, and long 2 ~ 3cm, middle reality, look even.It is high that pocket mushrooms belongs to nutrition, the heath food that heat is low, and containing trace elements such as crude protein, carbohydrate, fiber, VitB1, riboflavin, nicotinic acid, calcium, iron, potassium, sodium, phosphorus, the content of its protein is higher than general vegetables.The amino acid contained kind of pocket mushrooms is very abundant, wherein the content of protein, all higher than the flat mushroom of current domestic cultivation, Twospore Mushroom, mushroom and straw mushroom, and human body eight kinds of indispensable amino acids all possess, and its content accounts for more than 35% of all total amino acid contents.So often eat Pleurotus geesteranus, HUMAN HEALTH can be strengthened.According to another studying confirmation recently, some physiologically active substances that Pleurotus geesteranus contains, have the synthesis bringing out Interferon, rabbit, improve immune function of human body, have anti-cancer, anticancer effect.Pleurotus geesteranus is fatty, starch is little, is the ultimate food of diabetics and obesity patient, also has the effect reducing cholesterol.People are called " heath food ", " safety food ".
Pocket mushrooms cap is abundant, and quality is tender and crisp, and delicious flavour quite gets consumer reception, and market demand is increasing, and therefore, cultivated area is increasing.At present, general corn stalk, weed tree sawdust or the cotton seed hulls of all adopting is that main raw material cultivates pocket mushrooms, but the plantation raw material resources of routine are fewer and feweri, and nutritive ingredient is single, content is also lower, affect output and the healthy nutritive value of pocket mushrooms, the existing raw material resources found new starting material to replace or supplement, become extremely urgent thing.Meanwhile, pocket mushrooms substratum is infected by miscellaneous bacteria easily, and productive rate is low, and quality is influenced, affects economic benefit.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of pocket mushrooms substratum, and this substratum can promote the growth of pocket mushrooms, improves output, strengthens its anti-oxidant and anticancer function.
For achieving the above object, the present invention adopts following technical scheme:
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 36 ~ 43 parts, bagasse 6 ~ 12 parts, 11 ~ 22 parts, rice bran, Chinese medicine additive 8 ~ 18 parts, 6 ~ 13 parts, wheat bran, 0.8 ~ 2.1 part, lime.
Chinese medicine additive of the present invention, be made up of the raw material of following weight part: purple perilla 20 ~ 40 parts, the flower bud of lily magnolia 10 ~ 20 parts, Japanese Honeysuckle 5 ~ 10 parts, Pyrola Herb 10 ~ 20 parts, Ginkgo Leaf 5 ~ 10 parts, Tuber Fleeceflower Stem 5 ~ 10 parts, the bighead atractylodes rhizome 30 ~ 50 parts, 10 ~ 20 parts, rascal, Semen Cassiae 5 ~ 11 parts, Rhizoma Cibotii 10 ~ 30 parts, Herba Houttuyniae 6 ~ 16 parts.
Preferably, pocket mushrooms substratum of the present invention, is made up of the raw material of following weight part: cotton seed hulls 39 parts, bagasse 9 parts, 16 parts, rice bran, Chinese medicine additive 13 parts, 9 parts, wheat bran, 2 parts, lime, 1.4 parts, lime.
Preferably, Chinese medicine additive of the present invention, is made up of the raw material of following weight part: purple perilla 30 parts, the flower bud of lily magnolia 15 parts, Japanese Honeysuckle 8 parts, Pyrola Herb 15 parts, Ginkgo Leaf 7 parts, Tuber Fleeceflower Stem 8 parts, the bighead atractylodes rhizome 40 parts, 15 parts, rascal, Semen Cassiae 8 parts, Rhizoma Cibotii 20 parts, Herba Houttuyniae 11 parts.
In Chinese medicine additive raw material of the present invention:
Purple perilla, the band branch tender leaf of Labiatae Perilla plant purple perilla Perillafrutescens (L.) Britt..Nature and flavor: pungent, temperature.Function cures mainly: expelling cold and relieving exterior syndrome, regulating the flow of QI to ease the stomach.For common cold caused by wind-cold, headache, cough, distension and fullness of the chest and abdomen.
The flower bud of lily magnolia is the dry flower of Magnoliacea plant Flos Magnoliae MagnoliabiondiiPamp., yulan MagnoliadenudataDesv. or Flos Magnoliae MagnoliasprengeriPamp..Nature and flavor: pungent, temperature.Function cures mainly: dispersing wind and cold, relieving stuffy nose.For headche due to wind-cold, nasal obstruction, nasosinusitis, turbid nasal discharge.
Japanese Honeysuckle is the perennial half evergreen winding bejuco of Caprifoliaceae, by opening as white at the beginning of Flos Lonicerae, after transfer yellow to.Nature and flavor: property sweet cold air fragrance, clearing away heat with drugs sweet in flavor and cold in nature and not injuring one's stomach, fragrance thoroughly reaches and can be eliminating evil.Function cures mainly: can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as the card such as body heat, dermexanthesis, spot, hot carbuncle carbuncle, swelling and pain in the throat, and equal Be very effective.
Pyrola Herb is the herb of Pirolaceae plant wintergreen or Pylora rotundolia etc.Nature and flavor: sweetness and bitterness, temperature.Function cures mainly: can qi-restoratives, kidney-nourishing, dispel rheumatism, promoting blood flow to regulate menstruation, the strong bone of kidney tonifying, cough-relieving, hemostasis; Cure mainly: main lumbago due to renal deficiency, rheumatic arthralgia, soft, the new chronic cough of muscles and bones impotence are coughed, spit blood, bleeding from five sense organs or subcutaneous tissue, uterine bleeding, traumatic hemorrhage.
Ginkgo Leaf is the dry leave of Ginkgoaceae plant Ginkgo biloba (maidenhair tree, Gong Sunshu) GinkgobilobaL..Nature and flavor: sweet, bitter, puckery are flat; The thoughts of returning home, lung channel.Function cures mainly: astringe the lung, and relievings asthma, promoting blood circulation and removing blood stasis, pain relieving.Cough for the deficiency syndrome of the lung and breathe heavily; Coronary heart disease, stenocardia, hyperlipidemia, anti-freezing is solid, has certain probability to improve memory.Though Ginkgo Leaf can stimulate circulation, prevention cardiovascular diseases, but can not be taken other simultaneously and control cardiovascular agent (such as acetylsalicylic acid), Semen Ginkgo extrac is concentrated granular shape, there is very strong scavenging free radicals and antioxygenation, flavonoid glycoside in Ginkgo Leaf, amino acid and Amino acid synthesis collagen protein composition are to human body beauty treatment, and check melanin grows, and keep skin gloss and elasticity to play no small effect.
Tuber Fleeceflower Stem is rattan or the leaf bine stem of polygonum multiflorum thunb.Nature and flavor: sweet micro-hardship are flat.Function cures mainly: nourish heart, and calms the nerves, and dredging collateral dispels the wind.Control insomnia, internal lesion caused by overexertion, hidrosis, deficiency of blood bodily pain, ulcer, scrofula, wind sore mange.
The bighead atractylodes rhizome is the dry rhizome of feverfew bighead atractylodes rhizome AtractylodesmacrocephalaKoidz..Nature and flavor: bitter, sweet, temperature.Function cures mainly: invigorating the spleen and benefiting QI, and stomach, eliminating dampness Li Shui.
Rascal is rutaceae orange CitrusreticulataBlanco and the dry young fruit of cultivated variety thereof or the pericarp of immature fruit.Nature and flavor: bitter, pungent, temperature.Function cures mainly: soothing the liver relieving stagnant Qi, long-pendingization that disappear is stagnant.For distending pain in the chest and hypochondrium, hernia, newborn core, acute mastitis, abdominal pain due to retention of food.
Semen Cassiae is the dry mature seed of leguminous plants Cassia tora CassiaobtusifoliaL. or little Cassia tora CassiatoraL..Nature and flavor: sweet, bitter, salty, are slightly cold.Function cures mainly: removing heat to brighten vision, relaxes bowel.For the puckery pain of hot eyes, the many tear of photophobia, have a headache dizzy, order is secretly failed to understand, constipation.
Rhizoma Cibotii is the rhizome of Dicksoniaceae plant Cibotium barometz (L.) J. Sm..Nature and flavor: bitter, sweet, warm in nature.Function cures mainly: invigorating the liver and kidney, strengthens the bone, wind-damp dispelling; Cure mainly deficiency of the liver and kindey, the acid of waist knee is cold, and leg is soft weak, and rheumatic arthralgia.Rhizoma cibotii belongs to strong muscles and bones medicine, has the dual function for the treatment of and keeping healthy to the disease that muscles and bones is not good for.
Herba Houttuyniae, have another name called the folding basal part of the ear, cut youngster's root, hog snout encircles, heartleaf houttuynia, Hakka is referred to as dog and pastes ear, and taxonomy belonging to dicotyledons Saururaceae heartleaf houttuynia and belongs to, is a kind of herbaceous plant with fishy smell.Nature and flavor: taste is pungent, cold.Function cures mainly: sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach strengthening and digestion promoting are treated in clearing heat and detoxicating, detumescence, by the lung carbuncle, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc. of controlling real heat, heat poison, damp evil, disease heat are trouble.
Beneficial effect of the present invention is:
1, the Chinese medicine additive compatibility of the present invention's interpolation is scientific and reasonable, synergy, containing a large amount of crude protein, crude fat, Mierocrystalline cellulose, xylogen, mineral substance, amino acid in raw material, can as the nutritive ingredient of pocket mushrooms, whole nutritional needss of pocket mushrooms growth can be met, the whole trace mineral element required for pocket mushrooms growth and VITAMIN can be met, in substratum, special trace mineral element and VITAMIN need not be added, reduce the amount of other major ingredient of substratum, reduce costs.
2, substratum of the present invention is also containing biologically active substances such as alkaloid, polysaccharide, terpene, saponinses, and it has antibacterial and effect that is sterilization, also has the effect of desinsection.
3, the growth-promoting substance that Chinese medicine additive of the present invention contains the unknown can promote that pocket mushrooms fully absorbs nutritive ingredient, improves the utilization ratio of medium nutrient content, promotes the growth of pocket mushrooms, improve output, improve nutrition and the local flavor of pocket mushrooms, after testing, aminoacids content increases simultaneously.
4, the Chinese medicine material that Chinese medicine additive of the present invention is used can absorb the heavy metal of specifying in substratum before inoculation, as lead, cadmium, arsenic, mercury etc., effective solution belongs to mushroom because of pocket mushrooms, there is heavy metal accumulation ability, the heavy metal ion be dissolved in water almost can be absorbed completely, easily cause the heavy metal content of pocket mushrooms to exceed standard, human body is eaten, and threatens the problem of HUMAN HEALTH.
5, the Chinese medicine additive that the present invention adds can strengthen the anticancer of pocket mushrooms and anti-oxidation efficacy.
6, the raw material that the present invention is used mostly is natural matter, environmental protection, and harmless, cost is low.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiments.
Embodiment 1
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 36 parts, bagasse 12 parts, 11 parts, rice bran, Chinese medicine additive 18 parts, 13 parts, wheat bran, 0.8 part, lime.
Wherein said Chinese medicine additive, be made up (mixing) of the raw material of following weight part: purple perilla 20 parts, the flower bud of lily magnolia 10 parts, Japanese Honeysuckle 5 parts, Pyrola Herb 20 parts, Ginkgo Leaf 10 parts, Tuber Fleeceflower Stem 5 parts, the bighead atractylodes rhizome 50 parts, 20 parts, rascal, Semen Cassiae 11 parts, Rhizoma Cibotii 10 parts, Herba Houttuyniae 16 parts.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 4 times of water, then add whole raw material weight 0.3% yeast, stir, ferment in the open one day, then add Chinese medicine additive and stir, sealing, control temperature is 35 DEG C of fermentations 2 days, pack, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Embodiment 2
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 37 parts, bagasse 10 parts, 14 parts, rice bran, Chinese medicine additive 15 parts, 11 parts, wheat bran, 1.1 parts, lime.
Wherein said Chinese medicine additive, be made up (mixing) of the raw material of following weight part: purple perilla 25 parts, the flower bud of lily magnolia 13 parts, Japanese Honeysuckle 7 parts, Pyrola Herb 17 parts, Ginkgo Leaf 9 parts, Tuber Fleeceflower Stem 6 parts, the bighead atractylodes rhizome 45 parts, 18 parts, rascal, Semen Cassiae 10 parts, Rhizoma Cibotii 15 parts, Herba Houttuyniae 13 parts.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 5 times of water, then add whole raw material weight 0.5% yeast, stir, ferment in the open one day, then add Chinese medicine additive and stir, sealing, control temperature is 34 DEG C of fermentations 1 day, pack, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Embodiment 3
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 39 parts, bagasse 9 parts, 16 parts, rice bran, Chinese medicine additive 13 parts, 9 parts, wheat bran, 1.4 parts, lime.
Wherein said Chinese medicine additive, be made up (mixing) of the raw material of following weight part: purple perilla 30 parts, the flower bud of lily magnolia 15 parts, Japanese Honeysuckle 8 parts, Pyrola Herb 15 parts, Ginkgo Leaf 7 parts, Tuber Fleeceflower Stem 8 parts, the bighead atractylodes rhizome 40 parts, 15 parts, rascal, Semen Cassiae 8 parts, Rhizoma Cibotii 20 parts, Herba Houttuyniae 11 parts.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 5 times of water, then add whole raw material weight 0.6% yeast, stir, ferment in the open one day, then add Chinese medicine additive and stir, sealing, control temperature is 33 DEG C of fermentations 1.5 days, pack, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Embodiment 4
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 41 parts, bagasse 8 parts, 18 parts, rice bran, Chinese medicine additive 11 parts, 8 parts, wheat bran, 1.7 parts, lime.
Wherein said Chinese medicine additive, is made up (mixing) of the raw material of following weight part: purple perilla 35 parts, the flower bud of lily magnolia 18 parts, Japanese Honeysuckle 9 parts, Pyrola Herb 12 parts, Ginkgo Leaf 6 parts, Tuber Fleeceflower Stem 9 parts, the bighead atractylodes rhizome 35 parts, 8 parts, rascal, Semen Cassiae 7 parts, Rhizoma Cibotii 25 parts, Herba Houttuyniae 9 parts.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 7 times of water, then add whole raw material weight 0.7% yeast, stir, ferment in the open one day, then add Chinese medicine additive and stir, sealing, control temperature is 32 DEG C of fermentations 1 day, pack, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Embodiment 5
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 43 parts, bagasse 6 parts, 22 parts, rice bran, Chinese medicine additive 8 parts, 6 parts, wheat bran, 2.1 parts, lime.
Wherein said Chinese medicine additive, be made up (mixing) of the raw material of following weight part: purple perilla 40 parts, the flower bud of lily magnolia 20 parts, Japanese Honeysuckle 10 parts, Pyrola Herb 10 parts, Ginkgo Leaf 5 parts, Tuber Fleeceflower Stem 10 parts, the bighead atractylodes rhizome 30 parts, 10 parts, rascal, Semen Cassiae 5 parts, Rhizoma Cibotii 30 parts, Herba Houttuyniae 6 parts.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 9 times of water, then add whole raw material weight 0.8% yeast, stir, ferment in the open one day, then add Chinese medicine additive and stir, sealing, control temperature is 30 DEG C of fermentations 2 days, pack, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Comparative example
A kind of pocket mushrooms substratum, is made up of the raw material of following weight part: cotton seed hulls 43 parts, bagasse 8 parts, 26 parts, rice bran, 8 parts, wheat bran, 2.1 parts, lime.
The preparation method of described pocket mushrooms substratum is: take whole raw material by proportional quantity, by cotton seed hulls, bagasse, rice bran, wheat bran and lime, add whole raw material weight 9 times of water, then add whole raw material weight 0.8% yeast, stir, ferment in the open one day, seal, control temperature is 30 DEG C of fermentations 2 days, pack again, usual manner sterilizing, namely obtains pocket mushrooms substratum.
Test example 1
With the pocket mushrooms culture medium culturing pocket mushrooms comparative result of pocket mushrooms substratum of the present invention and comparative example, in table 1:
Table 1 contains the pocket mushrooms comparative result of Chinese medicine additive and comparative example culture medium culturing
Test example 2: substratum of the present invention is to the antioxygenation of the pocket mushrooms cultivated
1, material: the pocket mushrooms that embodiment 1, embodiment 3 and comparative example are cultivated respectively.
2, test method:
(1) pocket mushrooms runic thing prepares extracting method: the pocket mushrooms 10g that Example 1, embodiment 3 and comparative example are cultivated respectively, pulverize respectively, by solid-liquid ratio 1:25 extracting in water, extract three times, united extraction liquid, is concentrated into certain volume, adds the alcohol settling of 3 times 95%, get supernatant concentration, drying, obtain pocket mushrooms crude extract for subsequent use.
(2) mensuration of reducing power: sample is by the Tripotassium iron hexacyanide (K 3fe (CN) 6) be reduced into yellow prussiate of potash (K 4fe (CN) 6), yellow prussiate of potash again with Fe 3+effect, generate ferriferro cyanide (Prussian blue), to detect the size that Prussian blue absorbancy represents reducing power at 700nm wavelength place, absorbancy is higher, and the reducing power of sample is stronger, and oxidation-resistance is stronger.At 700nm wavelength place, measure that concentration is the embodiment 1 of 5mg/ml respectively, the light absorption value of pocket mushrooms crude extract that embodiment 3 and comparative example are cultivated respectively.
(3) superoxide anion experiment is removed: at generation ultra-oxygen anion free radical (O 2-) reaction system in, add the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example that concentration is 5mg/ml are cultivated respectively respectively, measure their clearance rates.
(4) experiment of hydroxyl radical free radical is removed: FeSO 4with H 2o 2reaction produces OH, and in system, add Whitfield's ointment, can catch hydroxyl radical free radical and produce coloring matter, this material has maximum absorption at 510nm place, and when there is OH free-radical scavengers in reaction system, this oxidising process is suppressed, and absorbance then reduces.At FeSO 4with H 2o 2in reaction system, add the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example that concentration is 30mg/ml are cultivated respectively respectively, measure clearance rate.
(5) NO under simulated gastric fluid pH condition 2-cleaning reaction effect: cleaning nitrite is one of approach effectively preventing N-nitroso compound carcinogenic.In simulated system, add the pocket mushrooms crude extract adding embodiment 1, embodiment 3 and comparative example that concentration is 14mg/ml respectively and cultivate respectively, measure clearance rate.
(6) DPPH method measures pocket mushrooms anti-oxidant activity: DPPH is a kind of stable free radical in organic solvent, is widely used in anti-oxidant appraisement system.In system, add the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example that concentration is 30mg/ml are cultivated respectively respectively, measure clearance rate.
3, test-results:
The reducing power of the pocket mushrooms crude extract that table 2 different culture media is cultivated
Embodiment 1 Embodiment 3 Comparative example
Light absorption value 1.532 1.542 0.834
Table 2 result shows, the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example are cultivated has oxidation-resistance, and embodiment 1 and embodiment 3 oxidation-resistance are better than comparative example.
The pocket mushrooms crude extract that table 3 different culture media is cultivated removes the ability of superoxide anion
Table 3 shows, the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example are cultivated all has the ability removing superoxide anion, and embodiment 1 and embodiment 3 Scavenging activity are better than comparative example.
The pocket mushrooms crude extract that table 4 different culture media is cultivated removes the ability of hydroxyl radical free radical
Embodiment 1 Embodiment 3 Comparative example
Clearance rate 78% 82% 44%
Table 4 shows, the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example are cultivated all has the ability removing hydroxyl radical free radical, and embodiment 1 and embodiment 3 Scavenging activity are better than comparative example.
The pocket mushrooms crude extract that table 5 different culture media is cultivated is to NO 2-scavenging(action)
Embodiment 1 Embodiment 3 Comparative example
Clearance rate 61% 65% 40%
Table 5 shows, the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example are cultivated is to NO 2-have scavenging(action), embodiment 1 and embodiment 3 Scavenging activity are better than comparative example.
The pocket mushrooms crude extract that table 6 different culture media is cultivated is to DPPH elimination effect
Embodiment 1 Embodiment 3 Comparative example
Clearance rate 65% 69% 32%
Table 6 shows, the pocket mushrooms crude extract that embodiment 1, embodiment 3 and comparative example are cultivated has elimination effect to DPPH, and embodiment 1 and embodiment 3 elimination effect are better than comparative example.
Comprehensive above-mentioned oxidizing reaction system test-results, the pocket mushrooms that known embodiment 1, embodiment 3 and comparative example are cultivated has oxidation-resistance, and embodiment 1 and embodiment 3 are better than comparative example, illustrate that the pocket mushrooms substratum adding additive of the present invention can strengthen the anti-oxidation efficacy of pocket mushrooms.
Test example 3: the pocket mushrooms antitumous effect that the present invention cultivates
1, laboratory animal: healthy Kunming mouse, body weight (22 ± 3) g, male and female half and half, are provided by Guangxi Medical University's Experimental Animal Center.
2, tumor cell line: murine sarcoma S 180ascit form, by Guangxi, treatment and prevention of tumour institute provides.
3, pocket mushrooms medicament preparation:
The pocket mushrooms 10g that turns out of Example 3 and comparative example respectively, be placed in the glass beaker of 1000mL, add water 500mL, after soaking 1h, be placed on fire and decoct, after first intense fire boils, change slow fire into, flame-out after decocting 60min again, by centrifugal for liquid 3500r/min 5min, clear liquid medicine in taking-up.And then 300mL water is added in sediment, decoct twice again with same method.Be placed on slow fire by 3 upper clear liquid medicine mixing, concentrated constant volume becomes 200mL, makes the original liquid containing crude drug 5%, is sealed by this liquid, is placed in refrigerator cold-storage and saves backup.
3, experimental technique:
Get (22 ± 3) g mouse 60, forelimb is subcutaneous inoculates the murine sarcoma S gone down to posterity the 7th day through kunming mouse respectively 180the abdominal cavity diluent 0.2mL of knurl strain cell (expecting that blue stain living cells number is greater than 95% through platform), its concentration 5 × 105/mL.Mouse after injection oncocyte is divided into 3 groups at random, often organizes 10, male and female half and half: embodiment 3 groups, homonymy coordination injection pocket mushrooms decoction diluent 0.25mL, whole dosage is every injected in mice pocket mushrooms composition 0.5mg, continuous note 10 times; Comparative example group, homonymy coordination injection pocket mushrooms decoction diluent 0.25mL, whole dosage is every injected in mice pocket mushrooms composition 0.5mg, continuous note 10 times; Control group, homonymy coordination injecting normal saline 0.5mL.Observe the Survival of the animation of mouse, tumor growth situation and mouse.
4, test-results:
From experiment, observe the weather of every mouse at any time.Control group mice starts to occur naked eyes Visible lumps (about 0.05cm × 0.05cm) for 1 week, and embodiment 3 groups, comparative example group then have no macroscopic lump.Embodiment 3 groups, comparative example group mouse are all survived.Continue to observe and within the 16th day, rise to experiment, control group mice starts dead gradually, all dead to 36 days control groups.Embodiment 3 groups, comparative example group mouse are then all survived, and are not all occurred naked eyes Visible lumps.Continue to observe to the 96th day, there is naked eyes Visible lumps in comparative example group mouse, continue to observe and within the 106th day, rise to testing, comparative example group mouse starts dead gradually, all dead to 126 days comparative example group mouse, embodiment 3 groups of mouse all survive, and do not occur naked eyes Visible lumps, continue to observe still all to survive to 160 days.This experiment repeats 4 times altogether, and result is substantially identical.The Survival of mouse is in table 7.
The pocket mushrooms anti-mouse S that table 7 different culture media is cultivated 180the experimental observations of ascitogenous sarcoma
Pocket mushrooms anticancer test result shows, the pocket mushrooms that embodiment 3 groups, comparative example are cultivated is to S 180ascitogenous sarcoma cell has restraining effect, and embodiment 3 groups is better than comparative example, illustrates that the pocket mushrooms substratum adding Chinese medicine additive of the present invention can strengthen the antitumous effect of pocket mushrooms.

Claims (3)

1. a pocket mushrooms substratum, is characterized in that, is made up of the raw material of following weight part: cotton seed hulls 36 ~ 43 parts, bagasse 6 ~ 12 parts, 11 ~ 22 parts, rice bran, Chinese medicine additive 8 ~ 18 parts, 6 ~ 13 parts, wheat bran, 0.8 ~ 2.1 part, lime;
Described Chinese medicine additive, be made up of the raw material of following weight part: purple perilla 20 ~ 40 parts, the flower bud of lily magnolia 10 ~ 20 parts, Japanese Honeysuckle 5 ~ 10 parts, Pyrola Herb 10 ~ 20 parts, Ginkgo Leaf 5 ~ 10 parts, Tuber Fleeceflower Stem 5 ~ 10 parts, the bighead atractylodes rhizome 30 ~ 50 parts, 10 ~ 20 parts, rascal, Semen Cassiae 5 ~ 11 parts, Rhizoma Cibotii 10 ~ 30 parts, Herba Houttuyniae 6 ~ 16 parts.
2. pocket mushrooms substratum according to claim 1, is characterized in that, is made up of the raw material of following weight part: cotton seed hulls 39 parts, bagasse 9 parts, 16 parts, rice bran, Chinese medicine additive 13 parts, 9 parts, wheat bran, 1.4 parts, lime.
3. pocket mushrooms substratum according to claim 1, is characterized in that, described Chinese medicine additive, be made up of the raw material of following weight part: purple perilla 30 parts, the flower bud of lily magnolia 15 parts, Japanese Honeysuckle 8 parts, Pyrola Herb 15 parts, Ginkgo Leaf 7 parts, Tuber Fleeceflower Stem 8 parts, the bighead atractylodes rhizome 40 parts, 15 parts, rascal, Semen Cassiae 8 parts, Rhizoma Cibotii 20 parts, Herba Houttuyniae 11 parts.
CN201511026340.2A 2015-12-31 2015-12-31 Pleurotus geesteranus medium Pending CN105384562A (en)

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