KR102012751B1 - Platycodon grandiflorum extract animal feed additive and animal feed - Google Patents

Abstract

The present invention relates to a bellflower extract animal feed additives and feed, it may be a bellflower extract, it may contain a bellflower extract as an active ingredient.

Description

Bellflower Extract Animal Feed Additive and Feed {PLATYCODON GRANDIFLORUM EXTRACT ANIMAL FEED ADDITIVE AND ANIMAL FEED}

The present invention relates to bellflower extract animal feed additives and feed.

In general, animal breeding is densely farmed in an artificial breeding system and is exposed to various forms of stress (high density farming, physical disorders, antibiotic and chemical abuse). For example, in the case of fish farming, it may interfere with the optimal growth of fish due to the above reasons, and cause negative changes in the metabolism and physiological state of the body, which is a problem in the seedling production and the positive process, ultimately reducing the production I'm bringing.

In addition, the nonspecific defense of animals is known as the first line of defense when infecting pathogens, and it has been reported that the ability to withstand pathogens can be enhanced by activating the innate immune system. Recently, researches on herbal medicines that can prevent disease and improve productivity by improving animal growth and feed efficiency or enhancing animal specific non-specific immune response and antioxidant capacity have been actively conducted.

Related prior art is patent 10-1072053 "Animal Enhancing Animal Feed Additives and Feeds Containing Root Extract". According to the prior art, the extract of Puerariae Radix is a receptor related to increased secretion capacity of NO, TNF-α, splenocyte proliferation effect, cytokine expression effect and innate immunity in macrophage Raw 264.7 cells. In addition to enhancing the activity of TLR-4 (Toll-like receptor 4) in immune cells, the immune system enhances the effects of rotavirus-induced intracellular immune activity and induces E. coli infection. It is disclosed that the lowering and immune system can be used as food, pharmaceutical and feed additives with excellent immunopotentiating efficacy in the prevention, inhibition and treatment of impaired immunodeficiency.

Patent No. 10-1072053 "Animal Enhancement Animal Feed Additives and Feedstuffs Carving Root Extract"

The present invention provides a bellflower extract animal feed additives and feed.

Technical problems to be achieved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned above may be clearly understood by those skilled in the art from the following description. There will be.

The present invention for achieving the above object may be a bellflower extract, it may contain a bellflower extract as an active ingredient.

According to the present invention, the animal's immunity is greatly improved by the feeding of the bellflower extract animal feed additive, and the productivity is improved, and the disease can be greatly prevented without the abuse of antibiotics, and mortality can be reduced.

In addition, saponin components are detected in the meat and eggs of animals ingesting bellflower extract animal feed additives, and when humans consume the meat or eggs, the saponin components are naturally absorbed, thereby improving health and improving health.

Hereinafter, the present invention will be described.

Bellflower is a Chinese word, Gilgyeong (백), Baekya (白藥), Goryeo (梗 草), and Kogyeong (苦 梗). The scientific name is Platycodon grandiflorum (JACQ.) A. DC. Bellflower grows in the plains of the temperate regions and in the sunny places of the mountains about 1,000m above sea level. The height of the stem is straight with 40-100cm. Roots are edible and grow thick and short.

Bellflower leaves are long oval, pointed end. In August-September, the flowers bloom in five parts, usually blue-purple or white. This is called a white bellflower, and the flowering of flowers is called a double bellflower. Bellflower root contains a lot of sugar, calcium and iron, and fiber is the main ingredient. So chewing taste is special, especially young roots of two or three years are very soft.

Digging from spring to autumn, raw food is eaten as it is, and dried and captured and eated from time to time. In terms of ingredients, autumn to early spring is good, but the bitterness is strong at this time.

The root of bellflower contains saponin and is used as a medicine. It first appeared in Hyangyakseongseongbang in the literature, “The taste is spicy, mild and slightly poisonous. He roots in February-August, and sun-dried soothes sore throats. ”

In Dong Bogam, “The quality is slightly cold, the taste is spicy, bitter and slightly poisonous. "It will control the lungs, throats, nose, and breasts and poison the bugs."

Ilhwa Maincho ruled the lungs' purulence and excreted farming. It is said that it is effective in cold, cough, cold sickness, abdominal pain, swelling, diarrhea, postpartum disease, women's disease, insomnia, infucate, tonsillitis, bronchitis, dysmenorrhea, dysentery, antitussive expectancy, hyperacidity, diuresis and blood.

Bellflower leaves are useful for burning feet, and boiled bellflowers and roosters are said to cure catheters. Toothache and diarrhea peel off the roots and oil to eat, and is also used to vomit blood.

In addition, the bellflower has been reported to verify various efficacy by modern scientific research. In other words, Platycodin D has been reported to prevent osteoclast formation and growth of breast cancer cells to prevent breast cancer causing bone destruction, and acute respiratory distress syndrome by inhibiting cell death and inflammation in the body and in vitro. It has been reported to weaken. Bellflower D has also been reported to inhibit tumor growth through angiogenesis inhibitory activity by protecting alcoholic liver disorders and blocking VEGFR2, which mediates signaling systems.

In addition, saponins extracted from bellflower, particularly Bellflower acid A, reduce egg white albumin-induced airway inflammation and PMA-exposed A549 cells, inhibit NF-kB and STAT1, and redox oxygen from Nrf2 / ARE-mediated hemoglobin. It has been reported to attenuate skin lesions such as atopic dermatitis through the activity of additive enzymes.

In addition, bellflower root extract inhibits UV B, which causes skin or hair pigmentation due to a decrease in Kit expression, and triterpenoid saponins isolated from bellflower inhibit hepatitis C replication, PI3K / Art, ERK1 / 2 and Saponin Extracted from Bellflower Root through Inhibition of Smad 2/3 Pathway on Human Lung Cancer Cell A549 Cells Inhibits TGFß1 Induces Epithelial Metastasis, It has been reported to modulate hepatic glucose, which inhibits enzymes and regulates enzymes in C57BL / KsJ-db / db mice.

In addition, saponins extracted from the roots of bellflower, Bellflower D (1), have been reported to have potential for adjuvant to specific cells and body immunity in response to recombinant avian influenza vaccine (rL-H5) based on Newcastle disease virus. Studies have been reported to conclude that Bellflower D (1) can improve the immunogenicity of the rL-H5 vaccine while improving humoral and cellular immune responses in rats, as well as a promising adjuvant for flu vaccines.

Animal feed additive of the present invention may be a bellflower extract, it may contain a bellflower extract as an active ingredient.

Animal feed additives of the present invention may be in the form of a powder or granules, may be administered in a mixed animal feed or mixed with a fluid such as water. For example, the animal feed additive of the present invention may be a grain feed such as milled or crushed wheat, oats, barley, corn and rice; Vegetable protein feed consisting mainly of rape, soybean and sunflower; Animal protein feeds such as blood meal, meat meal, bone meal and fish meal; It can be mixed with sugars and dairy feeds composed of various powdered milk and whey powder and the like, and administered to animals.

In addition, the animal feed additive of the present invention may be formed in a liquid form, and may be administered by mixing with an animal feed or a fluid such as water in an immersion or spray form. For example, it can be mixed and administered to an animal by spraying it on grain feed, vegetable protein feed, or animal protein feed.

In addition, the animal feed additives of the present invention may be administered to animals in admixture with animal feed additives such as nutritional supplements, digestion and absorption enhancers, growth promoters, disease prevention agents and the like.

Such animal feed additives of the present invention can be used in a variety of animal diets, including mammals, poultry and fish. For example, mammals, such as a pig, a cow, a sheep, a goat, a horse, a dog, and a rabbit; Poultry such as chickens, ducks, pheasants, turkeys, geese, quails and flowers; And fish such as trout, carp, flounder, flatfish, rockfish, leopard rock, dome, eel, loach, catfish, smelt and the like.

Example 1 Preparation of Bellflower Extract Animal Feed Additives

First washing with water without removing the bellflower shell, and twice with an air bubble washer. Next, after washing twice with an ultra-high pressure jet nozzle washing machine, it was dehydrated with a dehydrator. After adding 10 kg of purified water to 10 kg of the prepared bellflower, the bellflower extract animal feed additive was prepared by heating at 100 ° C. for 10 hours under a pressure of 1.5 kg / cm 2.

Example 2 Preparation of Bellflower Extract Animal Feed Additives

First washing with water without removing the bellflower shell, and twice with an air bubble washer. Next, after washing twice with an ultra-high pressure jet nozzle washing machine, it was dehydrated with a dehydrator. After adding 5 kg of purified water to 10 kg of the prepared bellflower, a bellflower extract animal feed additive was prepared by heating at 100 ° C. for 8 hours under 1.5 kg / cm 2 pressure.

Example 3 Preparation of Bellflower Extract Animal Feed Additives

First washing with water without removing the bellflower shell, and twice with an air bubble washer. Next, after washing twice with an ultra-high pressure jet nozzle washing machine, it was dehydrated with a dehydrator. 0.5 kg of vinegar (6% acetic acid) was added to 2 kg of the prepared bellflower, and the mixture was left at room temperature for 90 minutes, and then heated at a low temperature of 65 ° C. for 3 hours under a pressure of 1 kg / cm 2, followed by primary cold extraction, and 85 ° C. medium temperature. Secondary medium temperature extraction by heating for 6 hours at, and 3 hours high temperature extraction by heating for 1 hour at 105 ℃ high temperature to prepare a bellflower extract animal feed additives. The animal feed additive was added to a heating vessel and heated at 100 ° C. for 20 minutes while the inlet of the heating vessel was opened to volatilize acetic acid contained in the additive.

Example 4 Preparation of Bellflower Extract Animal Feed Additives

The bellflower extract prepared in Example 2 was introduced into a vacuum low pressure concentrator to increase the temperature of the concentrator to 70 ° C., and then maintained the vacuum while slowly adjusting the air inlet to -0.06 MPa. Thereafter, the temperature of the concentrator was maintained at 60 ° C. under vacuum, and then concentrated under a reduced pressure of -0.07 MPa for 6 hours while adjusting the intake amount of the air inlet, thereby preparing a bellflower extract animal feed additive.

Example 5 Preparation of Bellflower Extract Animal Feed Additives

The bellflower extract prepared in Example 3 was added to a vacuum condenser, heated to 80 ° C., and then concentrated under reduced pressure to −0.06 MPa to maintain vacuum, and the temperature of the concentrator was reduced to 55 ° C. under a reduced pressure of −0.07 MPa. Concentrate for 15 hours to prepare a bellflower extract animal feed additive.

Example 6 Preparation of Bellflower Extract Animal Feed Additives

Inoculated with 2g of Lactobacillus bacteria in 98g of bellflower extract prepared in Example 4 and stirred for 2 hours / hr stirring at 40 ° C. for 20 hours to prepare a bellflower extract animal feed additive.

Example 7 Preparation of Bellflower Extract Animal Feed Additives

The seedling animal feed additive was prepared by inoculating 2 g of Lactobacillus bacterium with 100 CFU / G of viable cell number to 98 g prepared in Example 5 and stirring and fermenting twice / hr for 20 hours at 40 ° C. temperature.

Example 8 Preparation of Animal Feed Additives Containing Bellflower Extract

Metabolism promoting and strong functional enhancers (product name: hepagin, GOLD BIO) 92% by weight of the bellflower extract prepared in Example 1 was mixed to prepare a bellflower extract-containing animal feed additives.

Example 9 Preparation of Animal Feed Additives Containing Bellflower Extract

A bellflower extract-containing animal feed additive was prepared by mixing 10 weight% of the bellflower extract prepared in Example 3 with 90% by weight of the liquid vitamin agent (product name: Vitaamino Gold solution, Meditech Co., Ltd.).

Example 10 Preparation of Bellflower Extract-Containing Animal Feed Additives

Powdered livestock probiotics (product name: Cheonsang-Bio 100 (Probiotic No. 1), Hongseong livestock bio) 10% by weight of the bellflower extract prepared in Example 2, mixed by spraying and drying the bellflower extract containing animal feed additives Was prepared.

Example 11 Preparation of Animal Feed Additives Containing Bellflower Extract

95% by weight of powdered probiotic (product name: Nokhyang Bio-II, Nohyang Fermentation Tech Agricultural Co., Ltd.) was sprayed, mixed with 5% by weight of the bellflower extract prepared in Example 6, and dried to prepare a bellflower extract-containing animal feed additive.

Preparation Example 1 Preparation of Trout Feed

Trout feed (product name: gold trout No. 5, suhyeop feed) 300 kg of the animal feed additive of Example 6 was mixed by spraying and then prepared by drying.

Preparation Example 2 Milking Feed Preparation

Milking feed (product name: milkbag No. 2, Hanil feed) 200 g of the animal feed additive of Example 1 was mixed by spraying and then prepared by drying.

Preparation Example 3 Scattering Feed Preparation

It was prepared by spraying 200 g of the animal feed additive of Example 2 to 3 kg of the scattered feed (product name: luxury gold scattering 115S, Nonghyup feed) and dried.

Preparation Example 4 Preparation of Broiler Feed

It was prepared by spraying 200g of animal feed additives of Example 2 to 3kg of broiler feed (product name: luxury premium P, Nonghyup feed) and dried.

<Form example 1>

The trout feed prepared in Preparation Example 1 was administered to a trout weighing 120 g twice a day in the morning and evening for one month.

Breeding Example 1

The milking feed prepared in Preparation Example 2 was administered to 7-year-old cows twice a day in the morning and evening for one month.

Breeding Example 2

The milking feed prepared in Preparation Example 2 was administered to 7-year-old cows twice a day for three months, morning and evening.

Poultry Example 1

The laying feed prepared in Preparation Example 3 was administered to a 55-day-old laying hen for two months, morning and evening, for three months.

Poultry Example 2

The broiler feed prepared in Preparation Example 4 was administered twice a day for breakfast and dinner for two months at 55 days of age.

Poultry Example 3

The broiler feed prepared in Preparation Example 4 was administered twice a day for breakfast and dinner for two months at 4 months of age.

Experimental Example 1 Fish Bottle Inspection

The trout farmed in Aquaculture Example 1 was tested by Chungcheongnam-do Fisheries Management Office. Bacterial and fungal diseases, parasitic diseases, and viral diseases were examined. The test results are shown in Table 1.

Inspection
result
Inspection items

test results
Bacterial, fungal diseases

Not detected
Parasitic diseases

Not detected






Viral diseases
Epidemic hematopoietic necrosis (EHN)

Not detected
Epidemic ulcer syndrome (EUS)

Not detected
Gyrodactylosis

Not detected
Infectious dysthymia (ISA)

Not detected
Viral hemorrhagic sepsis (VHS)

Not detected
Diagnosis Name
No infectious agent was detected for the test item

Experimental Example 2 Saponin Content Test

The saponin content of the trout farmed in Aquaculture Example 1 was examined by requesting the analysis room of the Hanpyeong Hansamin plant. Saponin content was tested from the unshelled and unshelled trout. First, divide the peeled and unshelled trout 160g each, and finely crushed using a blender. Place the sample in an Erlenmeyer flask, basify with 1 L of water, install a Liebig cooling tube and heat it to 100 ° C using an electric range to obtain each extract. Each extract was subjected to primary filtration using an 80 mesh sieve and secondary filtration using a vacuum filter (using filter paper No. 2 (5 μm)). Saponin content was examined from the filtered extract, and the test results are shown in Table 2.

sample

Saponin Content (mg / g)
Shelled trout

0.44
Unshelled trout

1.60

Experimental Example 3

The somatic cell count was examined by requesting Chungcheongnam-do Province Atomic Hygiene Research Institute. Somatic cell counting was requested from the milk milked from cows before breeding and the milk milked from cows after breeding. The test results are shown in Table 3.

sample

Somatic cell count (pieces / ml)
Crude milk milked before breeding

6.2 million
Crude milk milked after breeding

40.2 million

Experimental Example 4

The saponin content of milk milked from cows raised in breeding example 2 was examined by requesting from the Ginseng Herb Research Institute (Gumsan) of Chungcheongnam-do Agricultural Research and Extension Services. The test results are shown in Table 4.

sample

Saponin Content (mg / 100g)
crude oil

10.99

Experimental Example 5

The saponin content of the eggs laid by the laying hens in poultry example 1 was examined by the ginseng herbal research institute (Gumsan) of Chungcheongnam-do Agricultural Research and Extension Services. Saponin content of raw eggs and egg zodiac was examined. The test results are shown in Table 5.

sample

Saponin Content (mg / 100g)
Egg Zidane

0.51
Raw eggs

5.29

Experimental Example 6

Saponin content of chickens broiled in Poultry Example 2 was examined by the Ginseng Herb Research Institute (Gumsan), Chungcheongnam-do Agricultural Research and Extension Services. The test results are shown in Table 6.

sample

Saponin Content (mg / 100g)
Local chicken

2.36

Experimental Example 7

It was submitted to the Chungcheongnam-do Livestock Sanitation Research Center for the pathogenicity of native chickens broiled in Poultry Example 3. The examination is performed through an autopsy and the results of the autopsy are shown in Table 7.

test results




Autopsy findings
-No unusual findings

Bacteria test

▶ Bacteria separation and drug test
-Isolated bacteria: Pathogenic microorganisms cannot be separated
-Sensitive Drugs: Not applicable

Virus scan

Genetic testing (PCR and RT-PCR)
-Leo virus (Reo), chicken infectious bronchitis (IB): negative
Poultry adenovirus (FADV), chicken infectious anemia (CAV): negative

Parasite testing
▶ Microscope
Coxidium (Eimeria spp.): Negative

Diagnosis Name
No unusual findings (no pathogen detected)

As described above, according to the present invention, the animal's immunity is greatly improved by the feeding of the bellflower extract animal feed additive, and the productivity can be improved, and the disease can be greatly prevented without the abuse of antibiotics, and mortality can be reduced.

In addition, saponin components are detected in the meat and eggs of animals ingesting bellflower extract animal feed additives, and when humans consume the meat or eggs, the saponin components are naturally absorbed, thereby improving health and improving health.

The present invention has been described with reference to the preferred embodiments, and those skilled in the art to which the present invention pertains to the detailed description of the present invention and other forms of embodiments within the essential technical scope of the present invention. Could be. Here, the essential technical scope of the present invention is shown in the claims, and all differences within the equivalent range will be construed as being included in the present invention.

Claims (5)

An additive for trout feed containing a fermented product prepared by fermenting bellflower extract with Lactobacillus as an active ingredient.
The method of claim 1,
The fermented product is characterized in that 5% by weight based on the total weight of the additive for trout feed,
Additives for trout feed.
Prepared by spraying and mixing the additive for trout feed of claim 1 or 2 to 10 parts by weight based on 100 parts by weight of trout feed,
Trout feed.
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