CN101810247A - Complex microbial chicken feed additive - Google Patents
Complex microbial chicken feed additive Download PDFInfo
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- CN101810247A CN101810247A CN200910248903A CN200910248903A CN101810247A CN 101810247 A CN101810247 A CN 101810247A CN 200910248903 A CN200910248903 A CN 200910248903A CN 200910248903 A CN200910248903 A CN 200910248903A CN 101810247 A CN101810247 A CN 101810247A
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- chicken
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- bacillus subtilis
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- lactobacillus acidophilus
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Abstract
The invention relates to the field of complex microbes, in particular to a complex microbial chicken feed additive, which comprises cadida atilis, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis. The feed additive can be obtained through culturing the raw materials for 60-72 hours at a temperature of 35-37 DEG C in a culture medium with 10-20 percent of epimedium extract. Based on parts by weight, the ratio of the cadida atilis, the enterococcus faecalis, the lactobacillus acidophilus and the bacillus subtilis is (1-3): (1-3): (1-3): (1-3). The product of the invention is rich in various nutrients, and when the product is applied, the chicken growth speed is high, the fur production is of high yield and high quality, and the egg yield is greatly improved. Since the epimedium extract has the resistance against bird flu and other viruses, the invention can greatly reduce the chicken feeding cost, improve the immune function of chickens, and enhance the capabilities of chickens in disease prevention, disease treatment and virus resistance.
Description
Technical field
What the present invention relates to is the complex microorganism field, specifically the composite microbial feed additive of a kind of chicken.
Background technology
In recent years, people have recognized the drawback that huge economic benefit back that antibiotic feed additive brought is hidden, the endogenous and the suprainfection that cause such as antibiotic, the chemical sproof generation of cell, residual etc. in immunity of livestock decline and livestock products and the environment to aquaculture, feed industry and animal and human health have produced negative influence, therefore in order to overcome the Antibiotic Additive drawback, substitute with growth promoting function constantly is developed, probiotic, compound sugar, it mainly represents Chinese herbal medicines etc., at present a lot of probioticses all are that the fermentation by bacterial classification makes, this single strain fermentation, resistivity for the disease of animal is very poor, can not reduce the death rate of animal, especially the control to the virosis of animal is difficult to, also some development Chinese herbal medicine and probiotic formulations form, but some Chinese herbal medicine has inhibition for probio, therefore the effect of sterilization has weakened playing a role of probio to a large extent.
Summary of the invention
The composite microbial feed additive that the purpose of this invention is to provide a kind of chicken.
For achieving the above object, the technical solution used in the present invention is:
The composite microbial feed additive of a kind of chicken, it is characterized in that: form by candida utili, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis, then the 35-37 ℃ of culture of cultivating 60-72 hour is feed addictive in the culture medium of the Shorthorned Epimedium P.E that contains 10%-20%, counts candida utili by weight: enterococcus faecalis: lactobacillus acidophilus: bacillus subtilis=1-3: 1-3: 1-3: 1-3.
Described culture medium is that agar 1g, beef extract 1g, yeast soak powder 2.5g, sucrose 35g, defoamer 1.5g, peptone 1g, Shorthorned Epimedium P.E 10-20g, and distilled water adds to 1000ml.pH6.0。121 ℃ of autoclavings 20 minutes, standby.。Above-mentioned gained feed addictive is mixed the back throw in the chicken feed, add above-mentioned feed addictive with the 0.5-1% of chicken feed total amount.
The advantage that the present invention had: the mixing ratio that the present invention determines various microorganisms in the beneficial flora according to different growth phases and the kind of chicken, the forsythia suspense extraction of adding 10%-20% in the fermentation culture of composite flora; Through the repeatedly clinical testing of fryer and laying hen, determine best adding proportion.The present invention adds the extract of the plant capsule of weeping forsythia, effectively strengthens the composition of chicken resistance against diseases, is composited with probio, promotes the growth of chicken.Feed addictive of the present invention has no side effect, and can improve the immunologic function of animal, reduces the death rate of animal, improves raiser's economic benefit.Feed addictive of the present invention contains the multiple multiple nutrients composition that is rich in, fast growth after chicken is fed, and it is many and good to produce fur, and egg production increases the width of cloth and adds.Forsythia suspense extraction has the effect of virus such as anti-avian influenza, can reduce chicken raising cost significantly, can greatly improve chicken immunologic function, strengthen chicken diseases prevention, cure the disease, the chicken feed addictive of anti-virus ability.
The specific embodiment
Embodiment 1
Feed addictive: candida utili, enterococcus faecalis, lactobacillus acidophilus and the bacillus subtilis that will breed after cultivating are counted candida utili by weight: enterococcus faecalis: lactobacillus acidophilus: bacillus subtilis=1: 1: 1: 1, then in containing the culture medium of 10% Shorthorned Epimedium P.E 35 ℃ cultivated 60 hours, be feed addictive
Described culture medium is that agar 1g, beef extract 1g, yeast soak powder 2.5g, sucrose 35g, defoamer 1.5g, peptone 1g, Shorthorned Epimedium P.E 10g, and distilled water adds to 1000ml, pH6.0,121 ℃ of autoclavings 20 minutes, standby.
Described candida utili, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis breed cultivation according to conventional method in following culture medium separately.
Described candida utili propagation is incubated at potato and soaks powder (commercial) 3g, and glucose 20g, agar powder 15g, distilled water add in the 1000ml culture medium, and 121 ℃ of autoclavings 20 minutes are standby.
Described enterococcus faecalis propagation is incubated at glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.
Described lactobacillus acidophilus propagation is incubated at glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.025%, agar 1.5-2% is in the pH6.5 culture medium, 121 ℃ of autoclavings 20 minutes, standby.Described bacillus subtilis propagation is incubated at peptone 10g, agar 20g, and sodium chloride 5g, meat soak powder 10g, and water adds to 1000ml, in the pH7.2 culture medium, 121 ℃ of autoclavings 20 minutes, standby.
Described Shorthorned Epimedium P.E is carried, from Qufu, Shandong Sheng Wang plant Co., Ltd.
Above-mentioned gained feed addictive is measured meat chicken production performance and immunity aspect, test is carried out in horse ear mountain, Shenyang City plant, this test is divided into jumpbogroup and groupuscule two parts with every group test chicken, and emphasis is measured above-mentioned feed addictive to meat chicken production performance and Immune Effects (referring to table 1, table 2).
The influence of table 1 feed addictive different disposal group fryer live-weight (/g)
As seen, test group is compared with control group from table 1, and weight differential is remarkable, and it is better to the production performance effect of fryer to add 0.5% feed addictive of the present invention.
Table 2 feed addictive is to the influence of immune organ index
Draw from experimental data, the feed addictive that adds 0.5% addition of the present invention in the daily feed can obviously improve spleen, the bursa of farbricius and thymus gland index of immunity, and promotes the fryer immune organ to grow, thereby improves the fryer premunition.
Embodiment 2
Feed addictive: candida utili, enterococcus faecalis, lactobacillus acidophilus and the bacillus subtilis that will breed after cultivating are counted candida utili by weight: enterococcus faecalis: lactobacillus acidophilus: bacillus subtilis==1: 2: 2: 1, the back in containing the culture medium of 15% Shorthorned Epimedium P.E 35 ℃ cultivated 60 hours, be feed addictive
Described culture medium is that agar 1g, beef extract 1g, yeast soak powder 2.5g, sucrose 35g, defoamer 1.5g, peptone 1g, Shorthorned Epimedium P.E 15g, and distilled water adds to 1000ml, pH6.0,121 ℃ of autoclavings 20 minutes, standby.
Above-mentioned gained feed addictive is measured laying hen production performance and immunity aspect, test is carried out in Kaiyuan City laying hen field, this test is divided into jumpbogroup and groupuscule two parts with every group test chicken, and emphasis is measured above-mentioned feed addictive to laying hen production performance and Immune Effects (referring to table 3, table 4, table 5).
Table 3 feed addictive is to the statistics of laying hen production performance
As can be seen, feed addictive of the present invention can improve the egg production of laying hen greatly from following table 3, reduces feedstuff-egg ratio, increases the profit of culturing laying hen, obtains bigger economic benefit.
ND hemagglutination inhibition antibody tire (log2) in the table 4 different days serum
As shown in Table 4: 350, during 380 ages in days in the test group serum ewcastle disease hemagglutination inhibition antibody tire and exceed control group 25.81% and 25.42% respectively.Feed addictive of the present invention can improve the laying hen resistance against diseases greatly.
The sick incidence of table 5 enteron aisle
As shown in Table 5, the sick morbidity of the enteron aisle of test group number, the sick incidence of disease of enteron aisle, death toll are compared with control group greatly with death rate and are reduced, and the sick incidence of disease of enteron aisle and death rate have reduced by 58.82% and 57.58% than control group respectively.Feed addictive of the present invention can reduce the generation of intestines problem, reduces the death rate.
Test as can be known thus, feed addictive of the present invention can significantly improve the rate of body weight gain of fryer, strengthens immunity and the premunition of fryer and laying hen, for poultry husbandry provides the culture resources of high-quality more, obtain higher economic benefit and social benefit, promote the healthy and stable development of poultry farming.
Embodiment 3
Feed addictive: candida utili, enterococcus faecalis, lactobacillus acidophilus and the bacillus subtilis that will breed after cultivating are counted candida utili by weight: enterococcus faecalis: lactobacillus acidophilus: bacillus subtilis==3: 2: 2: 1, the back in containing the culture medium of 20% Shorthorned Epimedium P.E 35 ℃ cultivated 60 hours, be feed addictive
Described culture medium is that agar 1g, beef extract 1g, yeast soak powder 2.5g, sucrose 35g, defoamer 1.5g, peptone 1g, Shorthorned Epimedium P.E 20g, and distilled water adds to 1000ml, pH6.0,121 ℃ of autoclavings 20 minutes, standby.
Claims (3)
1. the composite microbial feed additive of a chicken, it is characterized in that: form by candida utili, enterococcus faecalis, lactobacillus acidophilus and bacillus subtilis, then the 35-37 ℃ of culture of cultivating 60-72 hour is feed addictive in the culture medium of the Shorthorned Epimedium P.E that contains 10%-20%, counts candida utili by weight: enterococcus faecalis: lactobacillus acidophilus: bacillus subtilis=1-3: 1-3: 1-3: 1-3.
2. press the composite microbial feed additive of the described chicken of claim 1, it is characterized in that: described culture medium is that agar 1g, beef extract 1g, yeast soak powder 2.5g, sucrose 35g, defoamer 1.5g, peptone 1g, Shorthorned Epimedium P.E 10-20g, distilled water adds to 1000ml.pH6.0。121 ℃ of autoclavings 20 minutes, standby.。
3. by the composite microbial feed additive of the described chicken of claim 1, it is characterized in that: above-mentioned gained feed addictive is mixed input afterwards with the chicken feed, add above-mentioned feed addictive with the 0.5-1% of chicken feed total amount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910248903A CN101810247A (en) | 2009-12-30 | 2009-12-30 | Complex microbial chicken feed additive |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910248903A CN101810247A (en) | 2009-12-30 | 2009-12-30 | Complex microbial chicken feed additive |
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| CN101810247A true CN101810247A (en) | 2010-08-25 |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228129A (en) * | 2011-08-04 | 2011-11-02 | 山东省明发同茂饲料有限公司 | Fermented feed |
| CN102294017A (en) * | 2011-08-01 | 2011-12-28 | 湖南农业大学 | Probiotics oral liquid for animal and preparation method thereof |
| CN103074281A (en) * | 2013-01-21 | 2013-05-01 | 黑龙江八一农垦大学 | Enterococcus faecalis FJL19 and application thereof |
| CN101812406B (en) * | 2009-12-30 | 2013-05-08 | 沈阳科丰牧业科技有限公司 | Composite microbial preparation for degrading aflatoxin |
| CN104206786A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Traditional Chinese medicine microecological preparation for meat chicken, preparation method and applications thereof |
| CN106983036A (en) * | 2017-04-24 | 2017-07-28 | 蚌埠农兴达现代农业有限公司 | A kind of feed of promotion broiler chicken thymus development |
| CN109303202A (en) * | 2018-12-05 | 2019-02-05 | 西北农林科技大学 | A kind of broiler chicken feed additive and preparation method thereof |
| CN109549018A (en) * | 2018-12-29 | 2019-04-02 | 广西万寿谷投资集团股份有限公司 | A kind of Chinese herbal medicine probiotic compound formulation for preventing and treating chicken coccidiasis |
| CN109645217A (en) * | 2018-12-29 | 2019-04-19 | 广西万寿谷投资集团股份有限公司 | A kind of zinc-rich probiotic and preparation method thereof improving free-ranging chicken immune function |
| CN112471350A (en) * | 2020-11-04 | 2021-03-12 | 河南森隆动物保健品有限公司 | Feed additive for improving laying performance of laying hens and reducing egg breakage rate and preparation method thereof |
| CN113142419A (en) * | 2021-05-19 | 2021-07-23 | 甘肃省畜牧兽医研究所 | Poultry feed additive and poultry feed additive powder, and preparation method and application thereof |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
-
2009
- 2009-12-30 CN CN200910248903A patent/CN101810247A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812406B (en) * | 2009-12-30 | 2013-05-08 | 沈阳科丰牧业科技有限公司 | Composite microbial preparation for degrading aflatoxin |
| CN102294017A (en) * | 2011-08-01 | 2011-12-28 | 湖南农业大学 | Probiotics oral liquid for animal and preparation method thereof |
| CN102294017B (en) * | 2011-08-01 | 2012-07-11 | 湖南农业大学 | Probiotics oral liquid for animal and preparation method thereof |
| CN102228129A (en) * | 2011-08-04 | 2011-11-02 | 山东省明发同茂饲料有限公司 | Fermented feed |
| CN102228129B (en) * | 2011-08-04 | 2013-01-23 | 山东省明发同茂饲料有限公司 | Fermented feed |
| CN103074281A (en) * | 2013-01-21 | 2013-05-01 | 黑龙江八一农垦大学 | Enterococcus faecalis FJL19 and application thereof |
| CN104206786A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Traditional Chinese medicine microecological preparation for meat chicken, preparation method and applications thereof |
| CN106983036A (en) * | 2017-04-24 | 2017-07-28 | 蚌埠农兴达现代农业有限公司 | A kind of feed of promotion broiler chicken thymus development |
| CN109303202A (en) * | 2018-12-05 | 2019-02-05 | 西北农林科技大学 | A kind of broiler chicken feed additive and preparation method thereof |
| CN109549018A (en) * | 2018-12-29 | 2019-04-02 | 广西万寿谷投资集团股份有限公司 | A kind of Chinese herbal medicine probiotic compound formulation for preventing and treating chicken coccidiasis |
| CN109645217A (en) * | 2018-12-29 | 2019-04-19 | 广西万寿谷投资集团股份有限公司 | A kind of zinc-rich probiotic and preparation method thereof improving free-ranging chicken immune function |
| CN112471350A (en) * | 2020-11-04 | 2021-03-12 | 河南森隆动物保健品有限公司 | Feed additive for improving laying performance of laying hens and reducing egg breakage rate and preparation method thereof |
| CN113142419A (en) * | 2021-05-19 | 2021-07-23 | 甘肃省畜牧兽医研究所 | Poultry feed additive and poultry feed additive powder, and preparation method and application thereof |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
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Open date: 20100825 |