CN102228129A - Fermented feed - Google Patents
Fermented feed Download PDFInfo
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- CN102228129A CN102228129A CN2011102222544A CN201110222254A CN102228129A CN 102228129 A CN102228129 A CN 102228129A CN 2011102222544 A CN2011102222544 A CN 2011102222544A CN 201110222254 A CN201110222254 A CN 201110222254A CN 102228129 A CN102228129 A CN 102228129A
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Abstract
The invention discloses a fermented feed. The fermented feed is prepared by the following steps of: performing amplification culture on Lactobacillus acidophilus ACCC10637 and Bacillus subtilis ACCC10619 respectively to obtain amplification culture solution by taking soybean whey as a raw material of a liquid culture medium, and performing amplification culture on Candida utilis ACCC20060 to obtain an amplification culture medium by a semi-solid culture medium; and according to the weight of a semi-solid-state fermentation culture medium, adding 3.2 percent of amplification culture solution of the Lactobacillus acidophilus ACCC10637, 1.0 percent of amplification culture medium of the Candida utilis ACCC20060, and 0.1 percent of amplification culture solution of the Bacillus subtilis ACCC10619 into the semi-solid-state fermentation culture medium respectively, and sealing and fermenting for 36 to 72 hours at the temperature of between 28 and 32 DEG C. When 10 percent of fermented feed is added into a pig feed, the utilization rate of the feed can be improved by 7 to 8 percent, the morbidity is reduced by 8 to 10 percent, and the culture environment is remarkably improved.
Description
Technical field
The present invention relates to a kind of fermented feed, belong to field of feed.
Background technology
Before 100 years, the bacteriologist Mei Qi of Nobel laureate Russia Elie Metchnikoff (Elie Metchnikoff) just proposes, and the bacterium in the middle of the fermented dairy product may can be used for controlling human intestine's bacterial fermentation process, thereby plays sanatory effect.Nowadays, people can buy sour milk, and the there face just contains profitable probliotics.
In the middle of 10 years, the notion of probio has also obtained application aspect Animal nutrition in the past.Can change intestinal microflora behind the microorganism oral administration sufficient dosage, and then the host is produced useful influence.Sum up several the tests of publishing, most results show that probio can play somatotrophic effect.But in the middle of feeding experiment, only some has observed significant facilitation effect.There is very big difference in this explanation different animals individuality to the reaction of this feed addictive.Probio is mainly realized somatotrophic effect by changing intestinal microflora, and its effect is relevant with swinery and the only individual central microbiotic concrete condition of pig.
Diarrhoea is topmost problem in the middle of first week behind the weaned piglet, thus people to alleviate the research of grice diarrhoea to how by probio also maximum.The data of publishing show that probio is handled all significantly decline of the incidence of disease of grice diarrhoea in the middle of 80% the test.No matter adopt which kind of probio (lactoenterococcus, Pediococcus acidilactici, lactobacillus acidophilus, lactobacillus lactis, Lactobacillus plantarum and Bacillus subtillis), all can observe this effect.
As some bacterial strain of additive for microbe feedstuff can secreting amylase, proteolytic enzyme and cellulase.In general, animal only is 20%～30% to the digestive utilization ratio of feed.This lower utilization rate is mainly caused by following two aspect reasons: 1. animal self enzyme system is incomplete, the enzyme quantity not sufficient.Higher amylase and the proteinase activity of omnivorous animal tool for example, but lack cellulase.Concerning some animal such as chicken, fish, the time that feed is digested and assimilated in body only is 3～4h simultaneously, and the reaction time is short.These conditions all make animal can not more food conversion be become nutrient digestion to absorb.2. the mixed feed that uses now is usually based on vegetable raw materials such as corn, bran, grouts, crop materials, and its crude fiber content is about 5%, and joint grain then can reach more than 15% for grain type feed.Except that the phytophage animal, other animals all can not digest and assimilate the material of cellulose, hemicellulose one class.In feed, add active bacteria formulation and can improve the utilization rate of animal effectively feed with higher exocrine enzymatic activity.The 8701a of separation such as what can make the efficiency of feed utilization raising 10.3% of growing and fattening pigs in early stage in Clear and Bright; When the dm423 that Kang Bai etc. separate adds in 0.02%～0.03% ratio, can improve efficiency of feed utilization 13.7%～18.9%.Simultaneously, the enzyme that adds of external source does not influence in the enteron aisle inulinase-producing activity of self microorganism.
Summary of the invention
Purpose of the present invention provides a kind of fermented feed.This fermented feed can be increased economic efficiency (promote that pig only grows, improve daily gain, reduce feedstuff-meat ratio), strengthens a pig resistance against diseases.
Technical scheme of the present invention is: a kind of fermented feed, it is characterized in that, be that liquid medium starting material enlarges respectively to cultivate to lactobacterium acidophilus ACCC10637, Bacillus subtillis ACCC10619 and becomes to spread cultivation liquid with the soybean whey liquid, adopt semisolid culturemedium that candida utili ACCC20060 is enlarged to cultivate and become to spread cultivation base; In the semi-solid ferment culture medium, add the lactobacterium acidophilus ACCC10637 liquid 3.2% that spreads cultivation respectively then by semi-solid ferment culture medium weight, candida utili ACCC20060 spread cultivation base 1.0% and the Bacillus subtillis ACCC10619 liquid 0.1% that spreads cultivation, under 28 ℃-32 ℃, carried out sealing and fermenting 36-72 hour; Beneficial microbe viable count in the described fermented feed 〉=1,000,000,000 cfu/g, pH≤5.0.
Described is that the fluid nutrient medium of raw material consists of (wt/wt) with the soybean whey liquid: add soybean whey liquid 5%, urea 0.5%, glucose 2.5%, K by water consumption
2HPO
40.25%, NaH
2PO
40.25%, MgSO
4.7H
2O 0.058%, MnSO
4.H
2O 0.025%, stirs sterilization.The main component of described soybean whey liquid is a soyabean oligosaccharides, content 2.5%-5%.
Described semisolid culturemedium consists of (wt/wt): inferior powder 62.5%, and wheat bran 4%, glucose 3.125%, molasses 1.25%, moisture 29.125% stirs.
Described semi-solid ferment culture medium consists of (wt/wt): inferior powder 5%, corn germ cake 20%, cotton dregs 10%, maize alcohol lees protein 15 %, dregs of beans 15%, water 30.7%, bacterial classification total amount 4.3%.
The preparation method:
(1) respectively lactobacterium acidophilus ACCC10637, candida utili ACCC20060 and Bacillus subtillis ACCC10619 are activated, and make shake-flask seed;
(2) be that liquid medium starting material enlarges respectively to cultivate to lactobacterium acidophilus ACCC10637, Bacillus subtillis ACCC10619 and becomes to spread cultivation liquid with the soybean whey liquid, inoculum concentration is 10%, condition of culture is: 37 ℃ of temperature, mixer rotating speed 100r/min, ventilating ratio is 1: 1, cultivates 36-72 hour;
(3) adopt semisolid culturemedium that candida utili ACCC20060 is enlarged cultivation, inoculum concentration is 10%, and mixer rotating speed 60r/min, ventilating ratio are 1: 1, and temperature was cultivated 48 hours down for 37 ℃;
(4) in the semi-solid ferment culture medium, add the lactobacterium acidophilus ACCC10637 liquid 3.2% that spreads cultivation respectively then by semi-solid ferment culture medium weight, candida utili ACCC20060 spread cultivation base 1.0% and the Bacillus subtillis ACCC10619 liquid 0.1% that spreads cultivation, under 28 ℃-32 ℃ condition, carried out sealing and fermenting 36-72 hour.
The invention has the beneficial effects as follows: the middle lactic acid bacteria of the fermented feed of the present invention preparation can effectively field planting in enteron aisle, produces superoxide dismutase, increases humoral immunity and cellular immunity, promotes growth.Saccharomycete and bacillus can produce cellulase, hemicellulase, the degradation of cell wall, promote the digestion of cellulosic material, impel nutriment stripping and for animal use to greatest extent, simultaneously can produce protease, lipase, amylase, vitamin etc., can effectively replenish the deficiency of enteron aisle endogenous enzymes, improve the digestion power of animal, and increase the feed palatability feed.Fermented feed enters enteron aisle and can adsorb noxious material and pathogen, reduces gastrointestinal disease, and the health that watches for animals reduces drug use.Owing to suppressed the breeding of pathogens such as spoilage organisms, improved the digestibility of nutrient in the feed simultaneously, reduced discharge of harmful gases such as ammonia, methane in a large number, plant's environment has obtained effective improvement.Therefore, fermented feed is the direction of advance that realizes healthy aquaculture and aquaculture sustainable development, has also effectively improved ecological benefits simultaneously, in pig feed, add 10% fermented feed, can make efficiency of feed utilization improve 7%-8%, the incidence of disease reduces 8%-10%, and breeding environment obviously improves.
The specific embodiment
Embodiment (one)
1. material
1.1 bacterial classification
Lactobacterium acidophilus ACCC10637, candida utili ACCC20060, Bacillus subtillis ACCC10619, Chinese agriculture microorganism fungus kind preservation center is buied.
1.2 actication of culture and shake a bottle culture medium
Lactobacterium acidophilus ACCC10637: with the activation of MRS agar medium, and carry out rejuvenation, with MRS Liquid Culture matrix manufacturing shake-flask seed.
Candida utili ACCC20060: with the activation of YPD Agr culture medium, and carry out rejuvenation, make shake-flask seed with malt extract medium.
Bacillus subtillis ACCC10619: with the activation of LB agar medium, and carry out rejuvenation, with LB Liquid Culture matrix manufacturing shake-flask seed.
1.3 bacterial classification is produced culture medium:
Be that liquid medium starting material enlarges respectively to cultivate to lactobacterium acidophilus ACCC10637, Bacillus subtillis ACCC10619 and becomes to spread cultivation liquid with the soybean whey liquid, inoculum concentration is 10%, condition of culture is: 37 ℃ of temperature, mixer rotating speed 100r/min, ventilating ratio is 1: 1, cultivates 36-72 hour.Number of viable is 5-10 hundred million cfu/ml after the medium culture.Culture medium is: add soybean whey liquid (wherein main component is a soyabean oligosaccharides, and content is 3%) 5%, urea 0.5%, glucose 2.5%, K by water consumption
2HPO
40.25%, NaH
2PO
40.25%, MgSO
4.7H
2O 0.058%, MnSO
4.H
2O 0.025%, stirs sterilization.
Adopt semisolid culturemedium that candida utili ACCC20060 is enlarged cultivation, inoculum concentration is 10%, and mixer rotating speed 60r/min, ventilating ratio are 1: 1, and condition of culture was cultivated 48 hours 37 ℃ of temperature.
It is 1-5 hundred million cfu/g that this medium culture saccharomycete obtains viable count.Described semisolid culturemedium consists of: inferior powder 62.5%, and wheat bran 4%, glucose 3.125%, molasses 1.25%, moisture 29.125% stirs.
2. semi-solid ferment is expected in fermentation
(1) semisolid culturemedium is formed by following weight ratio
Inferior powder 5%, corn germ cake 20%, cotton dregs 10%, maize alcohol lees protein 15 %, dregs of beans 15%, water 30.7%, bacterial classification total amount 4.3%.
(2) connect bacterium and fermentation parameter
Add the lactobacterium acidophilus ACCC10637 liquid 3.2% that spreads cultivation respectively by semi-solid ferment culture medium weight ratio, spread cultivation base 1.0% and Bacillus subtillis ACCC10619 of candida utili ACCC20060 spreads cultivation and carried out sealing and fermenting 72 hours under the condition of 0.1%, 30 ℃ of liquid.
Embodiment (two):
Choose that age in days is close, the healthy piglets of 96 DLY three way crosses of body weight (18 ± 1) kg, be divided into 2 groups at random, i.e. test group and control group, 48 every group, every group of 4 repetitions, 12 of every repetitions, each organizes male and female half and half.The test group common complete diet pellet of feeding, test group add fermented feed (being to add 1 kilogram of fermented feed in per 9 kilograms of common complete diet pellets) in 10% ratio in common complete diet pellet.Each step-by-step test time is piggy 34 days, middle pig 35 days, big pig 28 days.
7 days preliminary trial periods, carried out the progressively transition that pig was only observed, prevents epidemic, displaces and compiled overbit and tests material.Just enter and try the phase, two test group are fed respectively and are tested daily ration accordingly, feed intake twice every day, a pig free choice feeding and drinking-water.Sweep the colony house health morning every day.Write down daily ingestion amount and pig health status only every day.When just trying preceding and off-test, weigh morning on an empty stomach, and add up remaining forage volume.
Initial weight, the end of writing down each test group is heavy, every day feed consumption, raise fate, daily gain and meat feed ratio and the required feed cost of per kilogram weightening finish.After big pig step-by-step test finished, each selected each one of male and female at random two test group, and the pig of body weight basically identical only carries out slaughter experiment, measures the comparison in lean meat percentage and yellowish pink pool.
1, data analysis
(1) a test pig body weight and average daily gain (seeing Table 1)
Each test group pig body weight (kg) of table 1 and daily gain (g)
Table 1 is each processed group pig average weight, net gain and daily gain.At the test initial stage, each organizes a pig body weight difference not remarkable (P＞0.05).The daily gain of making a concrete analysis of each stage uses the test group of fermented feed of the present invention to be better than control group as can be known; Testing full phase control group and test group daily gain is respectively: 755 grams, 820 grams; The test group daily gain is higher by 8.61% than control group, and difference reaches the level of signifiance (P＜0.05).Use fermented feed significantly to promote pig only to grow in this explanation grow-finish swine rations, improve daily gain.
(2) a test pig feed consumption and feed conversion rate
Table 2 each a test group pig feed consumption (kg/ head) and feedstuff-meat ratio situation
As shown in Table 2, duration of test control group, test group feedstuff-meat ratio are respectively 2.95,2.74, and the test group price of deed is better than control group, and feedstuff-meat ratio has reduced by 7.12%.
Find in the experiment that when just having begun to feed fermented feed, the situation of searching for food of experimental group is very vigorous, obviously like to eat fermented feed.
(3) overall economic efficiency analysis
The cost that calculates piggy, middle pig, big three stage of pig mixed feed by duration of test feedstuff valency is respectively 1654 yuan/ton, 1544 yuan/ton, 1482 yuan/ton, calculate control group and test group duration of test per kilogram weightening finish feed cost and be respectively 4.56 yuan, 4.23 yuan, test group per kilogram weightening finish feed cost has reduced by 0.33 yuan than control group, and economic benefit has improved 7.24%.
(4) comparison of premunition
Fermented feed is to the influence of the pig incidence of disease
As can be known, on the diarrhoea degree, the diarrhoea of test group is lighter, and the control group grice diarrhoea is more serious, shows that fermented feed has tangible effect for the incidence of disease, the alleviating diarrhoea symptom that reduce grice diarrhoea, also better healing when treating relatively.
Claims (3)
1. fermented feed, it is characterized in that, be that liquid medium starting material enlarges respectively to cultivate to lactobacterium acidophilus ACCC10637, Bacillus subtillis ACCC10619 and becomes to spread cultivation liquid with the soybean whey liquid, adopt semisolid culturemedium that candida utili ACCC20060 is enlarged to cultivate and become to spread cultivation base; In the semi-solid ferment culture medium, add the lactobacterium acidophilus ACCC10637 liquid 3.2% that spreads cultivation respectively then by semi-solid ferment culture medium weight, candida utili ACCC20060 spread cultivation base 1.0% and the Bacillus subtillis ACCC10619 liquid 0.1% that spreads cultivation, under 28 ℃-32 ℃, carried out sealing and fermenting 36-72 hour.
2. a kind of fermented feed as claimed in claim 1 is characterized in that, described is that the fluid nutrient medium of raw material consists of with the soybean whey liquid: add soybean whey liquid 5%, urea 0.5%, glucose 2.5%, K by water consumption
2HPO
40.25%, NaH
2PO
40.25%, MgSO
4.7H
2O 0.058%, MnSO
4.H
2O 0.025%, stirs sterilization; Described semisolid culturemedium consists of: inferior powder 62.5%, and wheat bran 4%, glucose 3.125%, molasses 1.25%, moisture 29.125% stirs; Described semi-solid ferment culture medium consists of: inferior powder 5%, corn germ cake 20%, cotton dregs 10%, maize alcohol lees protein 15 %, dregs of beans 15%, water 30.7%, bacterial classification total amount 4.3%.
3. a method for preparing the described fermented feed of claim 2 is characterized in that, may further comprise the steps,
(1) respectively lactobacterium acidophilus ACCC10637, candida utili ACCC20060 and Bacillus subtillis ACCC10619 are activated, and make shake-flask seed;
(2) be that liquid medium starting material enlarges respectively to cultivate to lactobacterium acidophilus ACCC10637, Bacillus subtillis ACCC10619 and becomes to spread cultivation liquid with the soybean whey liquid, inoculum concentration is 10%, condition of culture is: 37 ℃ of temperature, mixer rotating speed 100r/min, ventilating ratio is 1: 1, cultivates 36-72 hour;
(3) adopt semisolid culturemedium that candida utili ACCC20060 is enlarged to cultivate and become to spread cultivation base, inoculum concentration is 10%, and mixer rotating speed 60r/min, ventilating ratio are 1: 1, and temperature was cultivated 48 hours down for 37 ℃;
(4) in the semi-solid ferment culture medium, add the lactobacterium acidophilus ACCC10637 liquid 3.2% that spreads cultivation respectively then by semi-solid ferment culture medium weight, candida utili ACCC20060 spread cultivation base 1.0% and the Bacillus subtillis ACCC10619 liquid 0.1% that spreads cultivation, under 28 ℃-32 ℃ condition, carried out sealing and fermenting 36-72 hour.
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| CN103609870A (en) * | 2013-10-08 | 2014-03-05 | 天津市畜牧兽医研究所 | Production method for big pig full-fermentation feed adopting DDGS as main protein raw material |
| CN103783318A (en) * | 2014-01-20 | 2014-05-14 | 肇庆市端州区广洪机电设备有限公司 | Fermented feed and processing process thereof |
| CN104431291A (en) * | 2014-11-27 | 2015-03-25 | 苏州嘉禧萝生物科技有限公司 | Fermented feed |
| CN104522295A (en) * | 2014-12-29 | 2015-04-22 | 邳州市东方养殖有限公司 | Method for preparing rabbit dung biological feed additive |
| CN104798986A (en) * | 2015-03-18 | 2015-07-29 | 生物源生物技术（深圳）有限公司 | Raw material semi-solid-state fermentation method and application thereof |
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| CN103609870A (en) * | 2013-10-08 | 2014-03-05 | 天津市畜牧兽医研究所 | Production method for big pig full-fermentation feed adopting DDGS as main protein raw material |
| CN103783318A (en) * | 2014-01-20 | 2014-05-14 | 肇庆市端州区广洪机电设备有限公司 | Fermented feed and processing process thereof |
| CN104855672A (en) * | 2014-02-26 | 2015-08-26 | 哈尔滨龙猪科技开发有限公司 | Preparation technology of rhzomorph granule feed |
| CN104431291A (en) * | 2014-11-27 | 2015-03-25 | 苏州嘉禧萝生物科技有限公司 | Fermented feed |
| TWI659696B (en) * | 2014-12-12 | 2019-05-21 | 南京莎菲特生物科技有限公司 | Method for preparing feed additive containing high-concentration surface hormone |
| CN104522295A (en) * | 2014-12-29 | 2015-04-22 | 邳州市东方养殖有限公司 | Method for preparing rabbit dung biological feed additive |
| CN104798986B (en) * | 2015-03-18 | 2018-04-10 | 生物源生物技术（深圳）股份有限公司 | A kind of raw material semi-solid ferment method and its application |
| CN104798986A (en) * | 2015-03-18 | 2015-07-29 | 生物源生物技术（深圳）有限公司 | Raw material semi-solid-state fermentation method and application thereof |
| CN104824409A (en) * | 2015-04-17 | 2015-08-12 | 荆楚理工学院 | Method used for preparing pig feed by taking sweet potato vine as raw material |
| CN104855730A (en) * | 2015-06-03 | 2015-08-26 | 铜陵县黑你养殖专业合作社 | Fermentation type chicken feed |
| CN104904994A (en) * | 2015-07-14 | 2015-09-16 | 谷实农牧集团股份有限公司 | Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof |
| CN105341467A (en) * | 2015-08-06 | 2016-02-24 | 铜陵百锐设备配件有限公司 | Fermentation type chicken feed |
| CN105341371A (en) * | 2015-08-06 | 2016-02-24 | 铜陵百锐设备配件有限公司 | Sheep feed |
| CN105053535A (en) * | 2015-08-08 | 2015-11-18 | 黄吉森 | Fermented crop straw composition and application thereof |
| CN105410410A (en) * | 2015-12-29 | 2016-03-23 | 雏鹰农牧集团股份有限公司 | Pig complete fermented feed and preparation method thereof |
| CN106538862A (en) * | 2016-11-02 | 2017-03-29 | 南京宝辉生物饲料有限公司 | A kind of biofermentation pig starter feed |
| CN108041297A (en) * | 2017-11-27 | 2018-05-18 | 河北京阳生物医药科技有限公司 | A kind of fermented pig feed and preparation method thereof |
| CN108497187A (en) * | 2018-03-28 | 2018-09-07 | 周口广安农牧科技有限公司 | A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans |
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