CN105614020B - A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof - Google Patents
A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof Download PDFInfo
- Publication number
- CN105614020B CN105614020B CN201510973171.7A CN201510973171A CN105614020B CN 105614020 B CN105614020 B CN 105614020B CN 201510973171 A CN201510973171 A CN 201510973171A CN 105614020 B CN105614020 B CN 105614020B
- Authority
- CN
- China
- Prior art keywords
- alfalfa
- livestock
- poultry
- forage additive
- microbial forage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 240000004658 Medicago sativa Species 0.000 title claims abstract description 130
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 title claims abstract description 126
- 239000004459 forage Substances 0.000 title claims abstract description 86
- 239000000654 additive Substances 0.000 title claims abstract description 85
- 230000000813 microbial effect Effects 0.000 title claims abstract description 85
- 230000000996 additive effect Effects 0.000 title claims abstract description 84
- 244000144972 livestock Species 0.000 title claims abstract description 75
- 244000144977 poultry Species 0.000 title claims abstract description 73
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 23
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 22
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 21
- 108010059892 Cellulase Proteins 0.000 claims abstract description 15
- 229940106157 cellulase Drugs 0.000 claims abstract description 15
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 14
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 14
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 14
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 13
- 229940085127 phytase Drugs 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 13
- 244000025254 Cannabis sativa Species 0.000 claims description 7
- 241000186660 Lactobacillus Species 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 4
- 235000010624 Medicago sativa Nutrition 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- 238000004519 manufacturing process Methods 0.000 abstract description 13
- 239000003674 animal food additive Substances 0.000 abstract description 8
- 230000000968 intestinal effect Effects 0.000 abstract description 4
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 230000035611 feeding Effects 0.000 description 38
- 241001465754 Metazoa Species 0.000 description 20
- 241000282887 Suidae Species 0.000 description 20
- 241000287828 Gallus gallus Species 0.000 description 17
- 241000283707 Capra Species 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 241000282849 Ruminantia Species 0.000 description 8
- 241000219793 Trifolium Species 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 230000037213 diet Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 229910052500 inorganic mineral Inorganic materials 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 239000011707 mineral Substances 0.000 description 5
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 239000002207 metabolite Substances 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 235000002949 phytic acid Nutrition 0.000 description 4
- 239000006041 probiotic Substances 0.000 description 4
- 235000018291 probiotics Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 150000003722 vitamin derivatives Chemical class 0.000 description 4
- 108010062877 Bacteriocins Proteins 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 239000000467 phytic acid Substances 0.000 description 2
- 229940068041 phytic acid Drugs 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000009654 wuzhi Substances 0.000 description 2
- 208000019901 Anxiety disease Diseases 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 101710138460 Leaf protein Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 235000004458 antinutrient Nutrition 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- 108700023668 bacilysin Proteins 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 208000018875 hypoxemia Diseases 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- -1 with yield is high Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a kind of livestock and poultry Alfalfa microbial forage additives and preparation method thereof, belong to feed additive field.Livestock and poultry Alfalfa microbial forage additive is made of the raw material mixed fermentation of fermenting microbe and following parts by weight: 550~700 parts of Alfalfa, 0.5~1.5 part of cellulase, 0.5~1.5 part of phytase；The dosage of the fermenting microbe are as follows: the Alfalfa of every 550~700g is corresponding to be added 40~60mL of Bacillus subtilis strain, 20~30mL of Bacillus licheniformis strain, 180~220mL of lactobacillus plantarum strain, 100~150mL of yeast seeds.It is livestock and poultry of the invention Alfalfa microbial forage additive nutritive value height, good absorbing, at low cost, and have the function of maintaining intestinal flora balance, enhancing immunity of livestock, for improving livestock and poultry production performance significant effect.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of livestock and poultry Alfalfa micro-ecological feed addition
Agent also relates to a kind of preparation method of livestock and poultry Alfalfa microbial forage additive.
Background technique
Clover is a kind of good leguminous forage, with yield is high, nitrogen fixing capacity is strong, quality is good, adaptable, palatability
Good feature, enjoys the good reputation of " King of Pasture ".Full of nutrition in clover, the crude protein content that contains is high, be 12.3%~
26.1%, general 18.5% or so, it is higher than the digestible protein that graminous pasture was harvested close to protein feed requirement
2.5 times or so；Clover is also the good source of minerals, about 6 times higher than the content of graminous pasture, especially rich in calcium, magnesium,
Potassium, wherein phosphorus is different from general vegetable raw material, is free of phytate phosphorus, and utilization rate is high；Simultaneously also containing there are many unknown growth factor
With the special dietaries functional component such as special immunoreactive protein such as leaf protein, flavones, polysaccharide, saponin, dietary cellulosic, for
Growth of animal, raising immunity of livestock, anticancer etc. is promoted to have good effect.Therefore, clover is used in livestock and poultry animal feed,
Feedstuff, the especially problem of protein feed anxiety can be alleviated to a certain extent, and animal products quality can be improved, it is full
Needs of the sufficient people to high-quality animal products.
Currently, Alfalfa is processed into ensilage mainly on Animal husbandry production to apply, and the key of ensiling is that control is former
Expect appropriate aqueous amount, and guarantee height anaerobism, moisture is excessively high, and unfavorable lactic acid bacteria inhibiting breeding, moisture is too low, does not easily cause and detests
Therefore oxygen environment when unfavorable to ensiling environmental requirement control, frequently can lead to the failure of Alfalfa ensiling.Moreover, with sunning
The extension of time, phytoprotein hydrolysis is serious, the serious nutritional quality for reducing ensilage.In addition, because clover crude fibre and
Content of lignin is more, and clover is usually used in ruminant feed at this stage, and assimilation effect is poor for most nonruminants, nutrition
It is not used.The present invention by by Alfalfa directly with enzyme, probiotics strain mixed fermentation, can greatly reduce environment influence because
Element, while multiple nutritional components can also be increased newly；The macromolecular substances for being difficult to digest in clover when fermentation, which can be also broken down into, to be easy to
The small-molecule substance of absorption improves the nutritive value of clover, is suitble to a variety of livestock and poultry edible；And storage convenient transportation.
Summary of the invention
The object of the present invention is to provide a kind of livestock and poultry Alfalfa microbial forage additive, which absorbs effect
Fruit is good, nutritional ingredient is high, good for improving livestock and poultry production performance effect.
Second purpose of the invention is to provide a kind of preparation method of livestock and poultry Alfalfa microbial forage additive.
In order to achieve the goal above, the technical scheme adopted by the invention is that:
A kind of livestock and poultry Alfalfa microbial forage additive, by fermenting microbe and the raw material of following parts by weight mixing hair
Ferment is made: 550~700 parts of Alfalfa, 0.5~1.5 part of cellulase, 0.5~1.5 part of phytase；The use of the fermenting microbe
Amount are as follows: the corresponding addition 40~60mL of Bacillus subtilis strain of the Alfalfa of every 550~700g, Bacillus licheniformis strain 20~
30mL, 180~220mL of lactobacillus plantarum strain, 100~150mL of yeast seeds.
Preferably, livestock and poultry Alfalfa microbial forage additive, by the original of fermenting microbe and following parts by weight
Material mixed fermentation is made: 600 parts of Alfalfa, 1 part of cellulase, 1 part of phytase；The dosage of the fermenting microbe are as follows: every 600g
Alfalfa corresponding Bacillus subtilis strain 60mL, Bacillus licheniformis strain 20mL, lactobacillus plantarum strain is added
220mL, yeast seeds 100mL.
The Bacillus subtilis strain viable count is 6.0~8.0 × 107CFU/mL, Bacillus licheniformis strain viable bacteria
Number is 3.0~4.0 × 107CFU/mL, lactobacillus plantarum strain viable count are 2.0~4.0 × 108CFU/mL, yeast seeds are living
Bacterium number is 2.0~4.0 × 108CFU/mL。
Preferably, the Bacillus subtilis strain viable count is 6.0 × 107CFU/mL, Bacillus licheniformis strain are living
Bacterium number is 4.0 × 107CFU/mL, lactobacillus plantarum strain viable count are 2.0 × 108CFU/mL, yeast seeds viable count are
4.0×108CFU/mL。
A kind of preparation method of above-mentioned livestock and poultry Alfalfa microbial forage additive, including take formula ratio raw material and
Fermenting microbe, after mixing, 28~42 DEG C of sealed fermentings 30~60 days up to livestock and poultry Alfalfa microbial forage additive.
Preferably, the condition of fermentation be 32 DEG C sealed fermenting 45 days.
Livestock and poultry of the invention Alfalfa microbial forage additive, the characteristic of each raw material are as follows:
Alfalfa: alfalfa, herbaceos perennial, crude protein content is high, while amino acid also rich in, mine
Substance and vitamin enjoy the good reputation of " King of Pasture " because it has the characteristics that grass yield is high, full of nutrition, palatability is good.
Cellulase: can act on cellulose and it is made to be decomposed into glucose, and cellulose is main group of cell wall
At part, therefore cellulase can play the role of rupturing plant cell wall, on the one hand be conducive to intracellular nutritional substance
Dissolution, on the other hand makes anti-nutritional factors degrade, and finally plays the effect for promoting plant feed to digest and assimilate.
Phytase: it can be the general name of inositol, phosphoric acid or phosphatic class of enzymes by phytic acid or phytate hydrolysis, can be improved
The biology utilization rate of plant phosphorus in diet eliminates the effect that phytic acid reduces mineral matter element potency, increases livestock and poultry feed intake,
Improve the production performance of animal.
Bacillus subtilis: being a kind of nontoxic, noresidue green feed additive, the effective component of preparation is with gemma shape
Formula exists, and metabolite mainly has protease, cellulase, bacteriocin etc., for improving the intestinal flora of livestock and poultry, improving animal
Production performance.Bacillus subtilis has growth promoting function to lactic acid bacteria, Bifidobacterium etc., these beneficial bacteriums mutually cooperate with conduct
One entirety plays a role.
Bacillus licheniformis: being a kind of nontoxic, noresidue green feed additive, the effective component of preparation is with gemma shape
Formula exists, and metabolite mainly has digestive ferment, vitamin and bacteriocin, can promote the life of normal physiological anaerobic bacteria in enteron aisle
It is long, it is mainly used for the production performance for improving the intestinal flora of livestock and poultry, improving animal.
Lactobacillus plantarum: being one kind of lactic acid bacteria, compared with this bacterium is the viable count of this bacterium with the difference of other lactic acid bacterias
Height, can largely produce acid, and the acidic materials of its output can degrade heavy metal, can the distinctive lactic acid of output in reproductive process
Bacillin, lactobacillin are a kind of preservatives of bion.
Saccharomycete: unicellular fungi, amphimicrobian.Mycoprotein, amino acid, vitamin and mineral equal size are abundant,
A large amount of thallus and metabolite can be not only generated in fermentation process, and have the function of improving tunning flavor.
Livestock and poultry of the invention Alfalfa microbial forage additive, by Alfalfa, enzyme and fermenting microbe mixed fermentation system
At.Wherein, Alfalfa crude protein rich in, crude fibre, crude fat, amino acid and minerals etc. are the substrates of fermentation,
By the effect of probiotics fermention and cellulase, phytase, plant cell wall and anti-nutrient substance are degraded, and are beneficial to nutrition
The absorption of substance, a large amount of thallus and trophism metabolite generated in fermentation process can be utilized by animal body；Withered grass gemma
The bacteriocin isoreactivity substance that can be generated in bacillus, Bacillus licheniformis body growth course, to pathogenic bacteria or autogenous infection
Pathogenic bacteria significantly inhibit, and can consume the free oxygen in enteron aisle rapidly, cause enteron aisle hypoxemia, and promotion is beneficial to be detested
The growth of oxygen bacterium；Plant cream bar bacillus can improve intestinal environment, promote digestion, inhibit pathogen, play and build up health, control disease
Generation effect；Saccharomycete can not only generate during the fermentation a large amount of mycoproteins, digestive ferment, amino acid, vitamin and
The nutritional ingredients such as minerals, and have the function of improving tunning flavor.The present invention is by by each raw material mixed fermentation, no
The absorption for only promoting Alfalfa nutriment improves its nutritive value；Also, resulting Alfalfa Tiny ecosystem is raised after fermenting
The probiotics contained in feed additives, which also has, adjusts enteron aisle balance, and pathogenic microorganism is inhibited to grow, and raising animal body is immune
Alfalfa probiotics are added to the production performance that can be good at improving animal in animal and fowl fodder by the effects of power.
The preparation method of livestock and poultry of the invention Alfalfa microbial forage additive, easy to operate, simple and easy, fermentation
Afterwards without harmful substances such as waste water, exhaust gas, utilization rate of fermenting is high, and feasibility is good；Raw material sources are wide, at low cost, are suitable for extensive
Chemical industry production, has good promotion and application value.
The usage of livestock and poultry of the invention Alfalfa microbial forage additive are as follows: be added in feed per ton of the invention micro-
50~150 kilograms of ecological feed additive feed.Alfalfa micro-ecological feed of the present invention is added in ruminant feed per ton to add
150 kilograms of agent are added to feed；80 kilograms of Alfalfa microbial forage additive throwings of the present invention are added in sow, growing and fattening pigs feed per ton
Hello；50 kilograms of Alfalfa microbial forage additive of the present invention are added in chicken feed per ton to feed.Each production phase whole-process application.
Specific embodiment
The present invention is further illustrated With reference to embodiment.
Embodiment 1
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 600g, cellulase 1g, phytase 1g, Bacillus subtilis strain 60mL, Bacillus licheniformis strain 20mL, plant cream bar
Bacterium strain 220mL, yeast seeds 100mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
6.0×107CFU/mL, Bacillus licheniformis strain viable count are 4.0 × 107CFU/mL, lactobacillus plantarum strain viable count are
2.0×108CFU/mL, yeast seeds viable count are 4.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after closing uniformly, 32 DEG C of sealed fermentings 45 days up to livestock and poultry Alfalfa microbial forage additive.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Embodiment 2
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 550g, cellulase 0.5g, phytase 0.5g, Bacillus subtilis strain 40mL, Bacillus licheniformis strain 30mL, plant
Lactobacillus strain 180mL, yeast seeds 150mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
8.0×107CFU/mL, Bacillus licheniformis strain viable count are 3.0 × 107CFU/mL, lactobacillus plantarum strain viable count are
4.0×108CFU/mL, yeast seeds viable count are 2.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after closing uniformly, 28 DEG C of sealed fermentings 60 days up to livestock and poultry Alfalfa microbial forage additive.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Embodiment 3
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 700g, cellulase 1.5g, phytase 1.5g, Bacillus subtilis strain 50mL, Bacillus licheniformis strain 25mL, plant
Lactobacillus strain 200mL, yeast seeds 120mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
7.0×107CFU/mL, Bacillus licheniformis strain viable count are 3.5 × 107CFU/mL, lactobacillus plantarum strain viable count are
3.0×108CFU/mL, yeast seeds viable count are 2.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after closing uniformly, 42 DEG C of sealed fermentings 40 days up to livestock and poultry Alfalfa microbial forage additive.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Embodiment 4
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 650g, cellulase 1.5g, phytase 1.0g, Bacillus subtilis strain 60mL, Bacillus licheniformis strain 30mL, plant
Lactobacillus strain 220mL, yeast seeds 100mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
8.0×107CFU/mL, Bacillus licheniformis strain viable count are 4.0 × 107CFU/mL, lactobacillus plantarum strain viable count are
3.0×108CFU/mL, yeast seeds viable count are 4.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after conjunction uniformly, 37 DEG C obtain livestock and poultry Alfalfa microbial forage additive in sealed fermenting 30 days to obtain the final product.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Embodiment 5
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 680g, cellulase 0.5g, phytase 1.5g, Bacillus subtilis strain 45mL, Bacillus licheniformis strain 20mL, plant
Lactobacillus strain 180mL, yeast seeds 130mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
8.0×107CFU/mL, Bacillus licheniformis strain viable count are 3.0 × 107CFU/mL, lactobacillus plantarum strain viable count are
4.0×108CFU/mL, yeast seeds viable count are 2.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after closing uniformly, 32 DEG C of sealed fermentings 45 days up to livestock and poultry Alfalfa microbial forage additive.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Embodiment 6
The livestock and poultry of the present embodiment Alfalfa microbial forage additive is made: Alfalfa of following raw material mixed fermentation
Powder 620g, cellulase 1g, phytase 0.5g, Bacillus subtilis strain 50mL, Bacillus licheniformis strain 20mL, plant cream
Bacillus species 200mL, yeast seeds 100mL.
The livestock and poultry of the present embodiment Alfalfa microbial forage additive, the Bacillus subtilis strain viable count are
6.0×107CFU/mL, Bacillus licheniformis strain viable count are 3.0 × 107CFU/mL, lactobacillus plantarum strain viable count are
4.0×108CFU/mL, yeast seeds viable count are 4.0 × 108CFU/mL。
The preparation method of the livestock and poultry of the present embodiment Alfalfa microbial forage additive, including taking the raw material of formula ratio mixed
Closed container is packed into after closing uniformly, 42 DEG C of sealed fermentings 30 days up to livestock and poultry Alfalfa microbial forage additive.
The usage of the livestock and poultry of the present embodiment Alfalfa microbial forage additive are as follows: be added in ruminant feed per ton
150 kilograms of gained Alfalfa microbial forage additive is fed；The gained micro- life of Alfalfa is added in sow, growing and fattening pigs feed per ton
80 kilograms of state feed addictive feed；50 kilograms of microbial forage additive of Alfalfa of gained is added in chicken feed per ton to feed.
Experimental example 1
Experiment is compared in Henan Province, broiler chicken field, Puyang County within 2015,2000 AA Broiler chicks are randomly divided into 8 groups,
Every group 250.The feed of 1~6 successively Alfalfa microbial forage additive of the feeding containing Examples 1 to 6 of group, method is as implemented
Example 1~6；Then feed of the feeding containing conventional Alfalfa, application method are identical as group 1~6 for group 7；Group 8 is blank group, and feeding is normal
Feed.42 ages in days are delivered for sale, and using being freely eaten and drinking water, immune programme and care procedure are carried out whole process by this normal procedure.
Chicken growth and incidence are recorded, as a result such as table 1.
1 each group growth of meat chicken situation of table compares
| Project | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7 | Group 8 |
| It tests number (only) | 250 | 250 | 250 | 250 | 250 | 250 | 250 | 250 |
| Average initial weight (gram) | 45.25 | 45.22 | 45.28 | 45.19 | 45.26 | 45.26 | 45.27 | 45.22 |
| Equal total augment weight (kilogram) | 2.48 | 2.46 | 2.44 | 2.45 | 2.38 | 2.36 | 2.02 | 2.18 |
| Equal total augment weight improves (%) | 13.76 | 12.84 | 11.93 | 12.39 | 9.17 | 8.26 | -7.34 | — |
| Feedstuff-meat ratio (F/G) | 1.70 | 1.71 | 1.73 | 1.73 | 1.72 | 1.76 | 2.12 | 1.95 |
| Feedstuff-meat ratio reduces (%) | 12.82 | 12.31 | 11.28 | 11.28 | 11.79 | 9.74 | -8.72 | — |
| The rate of animals delivered to the slaughter-house (%) | 99.60 | 99.20 | 99.60 | 98.80 | 98.40 | 98.00 | 90.40 | 94.40 |
| The rate of animals delivered to the slaughter-house improves (%) | 5.20 | 4.80 | 5.20 | 4.40 | 4.00 | 3.60 | -4.00 | — |
(note: equal total augment weight raising value, feedstuff-meat ratio decreasing value, rate of animals delivered to the slaughter-house raising value are each test group relative to blank group
Calculate gained)
The result shows that compared to the blank group, after feeding contains livestock and poultry of the present invention Alfalfa microbial forage additive, broiler chicken
Average total augment weight improves 8.26~13.76%, averagely improves 11.39%；Feedstuff-meat ratio reduces by 9.74~12.82%, averagely material meat
Than reducing by 11.54%；The rate of animals delivered to the slaughter-house improves 3.60~5.20%, and the average rate of animals delivered to the slaughter-house improves 4.53%, shows that livestock and poultry of the invention are used
Alfalfa microbial forage additive, which adds in broiler fodder, has better effects to the raising of meat chicken growth performance.
Experimental example 2
Experiment is compared on Henan Province, pig farm, Nanyue County within 2015,1600 growing and fattening pigs are randomly divided into 8 groups, every group
200.The feed of 1~6 successively Alfalfa microbial forage additive of the feeding containing Examples 1 to 6 of group, method such as embodiment 1
~6；Then feed of the feeding containing conventional Alfalfa, application method are identical as group 1~6 for group 7；Group 8 is blank group, and feeding is normally raised
Material.Experimental period 30 days, the growing state of experiment pig is recorded, as a result such as table 2.
2 each group growing-finishing pigs situation of table compares
| Project | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7 | Group 8 |
| It tests number (head) | 200 | 200 | 200 | 200 | 200 | 200 | 200 | 200 |
| Average initial weight (kilogram) | 36.12 | 36.15 | 35.92 | 36.08 | 35.93 | 36.27 | 36.20 | 36.19 |
| Average end weight (kilogram) | 61.17 | 61.13 | 60.83 | 60.95 | 60.86 | 61.15 | 57.09 | 57.73 |
| Equal total augment weight (kilogram) | 25.05 | 24.98 | 24.91 | 24.87 | 24.93 | 24.88 | 20.89 | 21.54 |
| Equal total augment weight improves (%) | 16.30 | 15.97 | 15.65 | 15.46 | 15.74 | 15.51 | -3.02 | — |
| Feedstuff-meat ratio (F/G) | 2.63 | 2.64 | 2.64 | 2.65 | 2.64 | 2.65 | 2.86 | 2.79 |
| Feedstuff-meat ratio reduces (%) | 5.73 | 5.38 | 5.38 | 5.02 | 5.38 | 5.02 | -2.51 | — |
(note: equal total augment weight raising value, feedstuff-meat ratio decreasing value are each test group relative to blank group calculating gained)
The result shows that compared to the blank group, after feeding contains livestock and poultry of the present invention Alfalfa microbial forage additive, test
In phase pig be averaged total augment weight improve 15.46~16.30%, averagely improve 15.77%；Feedstuff-meat ratio reduces by 5.02~5.73%, puts down
Equal feedstuff-meat ratio reduces by 5.32%.Show livestock and poultry of the invention with Alfalfa microbial forage additive to growing-finishing pigs performance
Raising has better effects.
Experimental example 3
Experiment is compared in Henan Province Wuzhi County mutton sheep within 2015,8 groups are randomly divided into 160 boer goats,
Every group 20.The feed of 1~6 successively Alfalfa microbial forage additive of the feeding containing Examples 1 to 6 of group, method is as implemented
Example 1~6；Then feed of the feeding containing conventional Alfalfa, application method are identical as group 1~6 for group 7；Group 8 is blank group, and feeding is just
Normal feed.Experimental period 30 days, the growing state of boer goat is recorded, as a result such as table 3.
3 each group boer goat growing state of table compares
| Project | Group 1 | Group 2 | Group 3 | Group 4 | Group 5 | Group 6 | Group 7 | Group 8 |
| Test belongs to (only) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| Average initial weight (kilogram) | 17.96 | 17.82 | 17.54 | 17.76 | 17.85 | 17.75 | 17.94 | 17.98 |
| Average end weight (kilogram) | 22.03 | 21.77 | 21.50 | 21.78 | 21.79 | 21.74 | 21.40 | 21.20 |
| Equal total augment weight (kilogram) | 4.07 | 3.95 | 3.96 | 4.02 | 3.94 | 3.99 | 3.46 | 3.22 |
| Equal total augment weight improves (%) | 26.40 | 22.67 | 22.98 | 24.84 | 22.36 | 23.91 | 7.45 | — |
| Feedstuff-meat ratio (F/G) | 2.08 | 2.09 | 2.12 | 2.09 | 2.11 | 2.11 | 2.36 | 2.58 |
| Feedstuff-meat ratio reduces (%) | 19.38 | 18.99 | 17.83 | 18.99 | 18.22 | 18.22 | 8.53 | — |
(note: equal total augment weight raising value, feedstuff-meat ratio decreasing value are each test group relative to blank group calculating gained)
The result shows that compared to the blank group, after feeding contains livestock and poultry of the present invention Alfalfa microbial forage additive, test
The boer goat total augment weight that is averaged improves 22.36~26.40% in phase, averagely improves 23.86%, and be significantly higher than feeding containing normal
Advise the 7.45% of Alfalfa feed group.Compared to the blank group, feeding Alfalfa microbial forage additive containing livestock and poultry of the present invention
Afterwards, boer goat feedstuff-meat ratio reduces by 17.83~19.38% in experimental period, and average feedstuff-meat ratio reduces by 18.61%, and significantly high
In feeding containing conventional Alfalfa feed group 8.53%.Show livestock and poultry of the invention with Alfalfa microbial forage additive to wave
You have very good effect at the raising of caprine growth performance.
Experimental example 4
Compare experiment on Henan Province, pig farm, Weizhi County within 2015, to 240 same periods breeding binary pregnant sows with
Machine is divided into 8 groups, every group 30.The feed of 1~6 successively Alfalfa microbial forage additive of the feeding containing Examples 1 to 6 of group,
Method such as Examples 1 to 66；Then feed of the feeding containing conventional Alfalfa, application method are identical as group 1~6 for group 7；Group 8 is blank
Group, feeding chow diet.Experimental period is gestation 42 days to weaned piglet, as a result the condition of production of minute book batch test sow is shown in
Table 4.
The production performance situation of 4 each group sow of table compares
(note: equal newborn piglet number raising value, life piglet number raising value, equal number pigs weaned raising value are each out
Test group calculates gained relative to blank group)
The result shows that compared to the blank group, after feeding contains livestock and poultry of the present invention Alfalfa microbial forage additive, test
The equal newborn piglet number of sow improves 7.87~10.06% in phase, averagely improves 8.77%, and is apparently higher than feeding and contains conventional lucerne
The 3.24% of Mu grass feed group.Compared to the blank group, feeding is tried containing after livestock and poultry of the present invention Alfalfa microbial forage additive
It tests the sow young number of just life in the phase and improves 16.80~19.50%, averagely improve 18.15%, hence it is evident that be higher than feeding containing conventional lucerne
The 7.19% of Mu grass feed group.Compared to the blank group, feeding is tried containing after livestock and poultry of the present invention Alfalfa microbial forage additive
It tests equal number pigs weaned in the phase and improves 13.31~16.68%, averagely improve 14.76%, and be apparently higher than feeding and contain conventional lucerne
The 8.80% of Mu grass feed group.Show that livestock and poultry of the invention are added to gestation with Alfalfa microbial forage additive and lactation is female
There are better effects to the raising of its production performance in pannage.
Experimental example 5
Henan Province, broiler chicken field, Wuzhi County compares test, 20000 AA Broiler chicks is randomly divided into 2 groups, respectively
Test group and control group, every group 10000, point 2 henhouse feedings.Alfalfa Tiny ecosystem of the test group feeding containing embodiment 1 is raised
The feed of feed additives, feeding method such as embodiment 1；Control group feeding chow diet.42 ages in days are delivered for sale, and whole process is used and freely adopted
Food and drinking-water, immune programme and care procedure are carried out by this normal procedure, record chicken growing state.As a result as follows, control
Average end weight (kg), feedstuff-meat ratio, the rate of animals delivered to the slaughter-house of group are as follows: 2.23,1.97,93.68%；The average end weight (kg) of test group, material meat
Than, the rate of animals delivered to the slaughter-house are as follows: 2.61,1.71,99.08%.Compared with the control group, the average end weight of test group and the rate of animals delivered to the slaughter-house are respectively increased
: 17.04% and 5.76%, feedstuff-meat ratio reduces 13.18%.
Experimental example 6
Henan Province, pig farm, Weizhi County compares test, is randomly divided into 2 groups to 60 same period breeding binary pregnant sows,
Respectively test group and control group, every group 30.The feeding of Alfalfa microbial forage additive of the test group feeding containing embodiment 2
Material, feeding method such as embodiment 2；Control group feeding chow diet, experimental period are gestation 42 days to weaned piglet, and minute book batch tries
Test the condition of production of sow.As a result as follows, the birth piglet number of control group, just live piglet number, equal number pigs weaned
(head) is respectively as follows: 12.40,11.17,9.60；The birth piglet number of test group, just live piglet number, equal number pigs weaned
(head) is respectively as follows: 14.03,13.07,12.03.Compared with the control group, the birth piglet number of test group, the first piglet that lives
Several, equal number pigs weaned is respectively increased: 13.15%, 17.01%, 25.31%.
Experimental example 7
Henan Province, pig farm, Weizhi County compares test, and the growing and fattening pigs that 600 counterpoises are about 36kg are randomly divided into 2
Group, respectively test group and control group, every group 300.Alfalfa microbial forage additive of the test group feeding containing embodiment 5
Feed, feeding method such as embodiment 5；Control group feeding Normal Goods feed, experimental period 30 days, minute book batch test growing and fattening pigs
Growing state.As a result as follows, average total augment weight (kg), the feedstuff-meat ratio of control group growing and fattening pigs are respectively 21.13,2.74；Test
Average total augment weight (kg), the feedstuff-meat ratio of group growing and fattening pigs are respectively 26.01,2.59.Compared with the control group, test group growing and fattening pigs is flat
Equal total augment weight improves 23.10%, and feedstuff-meat ratio reduces by 5.47%.
Experimental example 8
Henan Province county, Weihui City mutton sheep compares test, by 200 initial weight that are averaged be about 18kg boer goat with
Machine is divided into 2 groups, respectively test group and control group, and every group 100, Alfalfa Tiny ecosystem of the test group feeding containing embodiment 1 is raised
The feed of feed additives, feeding method such as embodiment 1；Control group feeding chow diet experimental period 30 days, records boer goat
Growing state.As a result as follows, average total augment weight (kg), the feedstuff-meat ratio of control group boer goat are respectively 3.15,2.63；Test group
Average total augment weight (kg), the feedstuff-meat ratio of boer goat are respectively 4.12,2.06.Compared with the control group, test group boer goat
Average total augment weight improves 30.79%, and feedstuff-meat ratio reduces by 21.67%.
Claims (5)
1. a kind of livestock and poultry Alfalfa microbial forage additive, it is characterised in that: by fermenting microbe and following parts by weight
Raw material mixed fermentation is made: 550~700 parts of alfalfa meal, 0.5~1.5 part of cellulase, 0.5~1.5 part of phytase；It is described
The dosage of fermenting microbe are as follows: the Alfalfa of every 550~700g is corresponding to be added 40~60mL of Bacillus subtilis strain, lichens gemma
20~30mL of bacillus species, 180~220mL of lactobacillus plantarum strain, 100~150mL of yeast seeds；The bacillus subtilis
Bacterium strain viable count is 6.0~8.0 × 107CFU/mL, Bacillus licheniformis strain viable count are 3.0~4.0 × 107CFU/mL、
Lactobacillus plantarum strain viable count is 2.0~4.0 × 108CFU/mL, yeast seeds viable count are 2.0~4.0 × 108CFU/
mL；
Livestock and poultry are made with Alfalfa microbial forage additive by the method included the following steps: taking raw material and the fermentation of formula ratio
Strain, after mixing, 28~42 DEG C of sealed fermentings 30~60 days up to livestock and poultry Alfalfa microbial forage additive.
2. livestock and poultry according to claim 1 Alfalfa microbial forage additive, it is characterised in that: by fermenting microbe and
The raw material mixed fermentation of following parts by weight is made: 600 parts of Alfalfa, 1 part of cellulase, 1 part of phytase；The fermenting microbe
Dosage are as follows: the Alfalfa of every 600g is corresponding to be added Bacillus subtilis strain 60mL, Bacillus licheniformis strain 20mL, plant
Lactobacillus strain 220mL, yeast seeds 100mL.
3. livestock and poultry according to claim 1 Alfalfa microbial forage additive, it is characterised in that: the withered grass gemma
Bacillus species viable count is 6.0 × 107CFU/mL, Bacillus licheniformis strain viable count are 4.0 × 107CFU/mL, plant cream bar
Bacterium strain viable count is 2.0 × 108CFU/mL, yeast seeds viable count are 4.0 × 108CFU/mL。
4. a kind of preparation method of livestock and poultry as described in claim 1 Alfalfa microbial forage additive, it is characterised in that:
Raw material and fermenting microbe including taking formula ratio, after mixing, 28~42 DEG C sealed fermenting 30~60 days up to livestock and poultry lucerne
Mu grass microbial forage additive.
5. the livestock and poultry according to claim 4 preparation method of Alfalfa microbial forage additive, it is characterised in that: hair
The condition of ferment be 32 DEG C sealed fermenting 45 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510973171.7A CN105614020B (en) | 2015-12-22 | 2015-12-22 | A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510973171.7A CN105614020B (en) | 2015-12-22 | 2015-12-22 | A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105614020A CN105614020A (en) | 2016-06-01 |
| CN105614020B true CN105614020B (en) | 2019-11-08 |
Family
ID=56029721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510973171.7A Expired - Fee Related CN105614020B (en) | 2015-12-22 | 2015-12-22 | A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105614020B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109380598A (en) * | 2017-08-07 | 2019-02-26 | 康盛京构（北京）科技有限公司 | A kind of paper mulberry ensilage and preparation method thereof, purposes |
| CN107865216B (en) * | 2017-11-16 | 2021-03-23 | 广西壮族自治区畜牧研究所 | Mulberry leaf biological feed and preparation method and application thereof |
| CN108552427A (en) * | 2018-04-19 | 2018-09-21 | 河南农科牧业有限公司 | A kind of nursery-stage pig feed containing chocolate powder |
| CN109258929A (en) * | 2018-10-16 | 2019-01-25 | 甘肃中丰农业科技有限公司 | A kind of Fermented Grass feed and its production method |
| CN110468064A (en) * | 2019-04-22 | 2019-11-19 | 河南中微生物科技研究院(有限合伙) | A kind of alfalfa ensilage agent and preparation method thereof |
| CN110291878B (en) * | 2019-07-10 | 2021-08-06 | 陕西理工大学 | Planting method of zinc-rich alfalfa, zinc-rich alfalfa feed additive and preparation method and application thereof |
| CN110810624A (en) * | 2019-11-22 | 2020-02-21 | 合肥五粮泰生物科技有限公司 | Preparation method of alfalfa fermented slurry feed |
| CN111034864A (en) * | 2019-12-31 | 2020-04-21 | 山西大禹生物工程股份有限公司 | Functional microbial additive for improving quality of silage and preparation method and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101518297B (en) * | 2008-12-26 | 2012-05-09 | 李仁煌 | Multiple microbial straw composite full-effect granulated feed and preparation method thereof |
| CN101700103A (en) * | 2009-09-17 | 2010-05-05 | 河南省农业科学院 | Core premix for organic beef cattle |
| CN103459604A (en) * | 2011-02-14 | 2013-12-18 | 希乐克公司 | Processing biomass |
-
2015
- 2015-12-22 CN CN201510973171.7A patent/CN105614020B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN105614020A (en) | 2016-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105614020B (en) | A kind of livestock and poultry Alfalfa microbial forage additive and preparation method thereof | |
| CN105475624B (en) | Microbial fermentation feed and production method and application thereof | |
| KR100840145B1 (en) | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof | |
| Gado et al. | Influence of exogenous enzymes ensiled with orange pulp on digestion and growth performance in lambs | |
| CN102696547B (en) | Method for breeding Fattening pigs | |
| CN102894215B (en) | Traditional Chinese medicine multi-enzyme bacterium straw composite feed for livestock and poultry | |
| CN102228129B (en) | Fermented feed | |
| CN103621766A (en) | Preparation method and application of biological feed additive with nutrition and immunocompetence | |
| CN103621979A (en) | Preparation and application of special compound premix for fermented feed | |
| CN106819481A (en) | A kind of full price fermented feed for piglet religion groove and preparation method thereof | |
| CN104970257A (en) | Antibiotic-free beef cattle growing and fattening premix and feed | |
| CN106509432A (en) | Piglet intestinal health improving and weaning stress reducing creep feed and preparation method thereof | |
| CN103798553A (en) | Environment-friendly pig feed additive and preparation method thereof | |
| CN104431560A (en) | Pig feed additive with efficacies of protecting intestinal tracts and improving food calling function | |
| CN108522869A (en) | A kind of environmentally friendly sucking pig mixed feed and preparation method thereof based on guarantee enteron aisle | |
| CN105053566A (en) | Cornus walteri seed proteinase feed additive and preparation method thereof | |
| CN104782982A (en) | Mouth-open feed for rearing calves and preparation method for mouth-open feed | |
| CN105360615A (en) | Fermented feed raw material preparation as well as preparation method and application thereof | |
| CN104886392A (en) | Biological fermentation pig feed replacing antibiotics, and preparation method of biological fermentation pig feed | |
| CN106071143B (en) | A kind of granular pattern probiotics and preparation method thereof that ruminant production performance can be improved | |
| CN107183372B (en) | Milk replacer for weaning meat calves | |
| CN107373132A (en) | Enzyme preparation live pig mixed feed | |
| CN105815549A (en) | Amino acid high protein powder feed additive and preparation method thereof | |
| CN108056260A (en) | Feed for beef cattle and preparation method thereof and the application in the special cultivation of Muslim beef | |
| KR102492755B1 (en) | Method for preparing fermented total mixed ration using microbial strain complex and steam treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191108 Termination date: 20211222 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |