CN110810624A - Preparation method of alfalfa fermented slurry feed - Google Patents
Preparation method of alfalfa fermented slurry feed Download PDFInfo
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- CN110810624A CN110810624A CN201911152786.8A CN201911152786A CN110810624A CN 110810624 A CN110810624 A CN 110810624A CN 201911152786 A CN201911152786 A CN 201911152786A CN 110810624 A CN110810624 A CN 110810624A
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- 238000000855 fermentation Methods 0.000 claims abstract description 39
- 230000004151 fermentation Effects 0.000 claims abstract description 39
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 13
- 239000002893 slag Substances 0.000 claims abstract description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 12
- 239000008103 glucose Substances 0.000 claims abstract description 12
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- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 9
- 241000193749 Bacillus coagulans Species 0.000 claims description 7
- 241000186660 Lactobacillus Species 0.000 claims description 7
- 229940054340 bacillus coagulans Drugs 0.000 claims description 7
- 229940039696 lactobacillus Drugs 0.000 claims description 7
- 244000063299 Bacillus subtilis Species 0.000 claims description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 3
- 108010059820 Polygalacturonase Proteins 0.000 claims description 3
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 3
- 239000011268 mixed slurry Substances 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 238000003860 storage Methods 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 230000000433 anti-nutritional effect Effects 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 241000283690 Bos taurus Species 0.000 description 10
- 235000013336 milk Nutrition 0.000 description 8
- 239000008267 milk Substances 0.000 description 8
- 210000004080 milk Anatomy 0.000 description 8
- 244000025254 Cannabis sativa Species 0.000 description 7
- 235000013365 dairy product Nutrition 0.000 description 6
- 230000006651 lactation Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
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- 241001465754 Metazoa Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000001082 somatic cell Anatomy 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 102000014171 Milk Proteins Human genes 0.000 description 2
- 108010011756 Milk Proteins Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- ZZIALNLLNHEQPJ-UHFFFAOYSA-N coumestrol Chemical compound C1=C(O)C=CC2=C1OC(=O)C1=C2OC2=CC(O)=CC=C12 ZZIALNLLNHEQPJ-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 235000021243 milk fat Nutrition 0.000 description 2
- 235000021239 milk protein Nutrition 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 102000015781 Dietary Proteins Human genes 0.000 description 1
- 108010010256 Dietary Proteins Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
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- 238000004806 packaging method and process Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
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- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
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- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
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- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a preparation method of alfalfa fermentation slurry feed, belonging to the technical field of feed, and the method comprises the following steps: 1) crushing and grinding fresh alfalfa, and then heating and sterilizing to obtain sterile alfalfa slurry; 2) the sterile alfalfa slurry enters extrusion separation equipment for solid-liquid separation to obtain dilute liquid and wet slag; 3) the dilute solution is inoculated with mixed bacteria agent 1, glucose and nutrient salt for fermentation; 4) adding the wet residue into a mixed microbial inoculum 2, a complex enzyme preparation, glucose and nutrient salt for fermentation; 5) mixing the fermented materials obtained in the step 3) and the step 4), and grinding uniformly after mixing to obtain the alfalfa fermentation slurry feed. The preparation method of the alfalfa fermentation slurry feed provided by the invention provides the alfalfa fermentation slurry feed which is rich in nutrition, is storage-resistant, has good absorption and does not contain anti-nutritional factors.
Description
Technical Field
The invention relates to the technical field of feeds, and particularly relates to a preparation method of alfalfa fermentation slurry feed.
Background
Alfalfa is a good name of the king of pasture, and is excellent pasture with the longest cultivation history and the widest cultivation area in the world. It is not only a good feed for herbivorous animals such as cattle, sheep, rabbits, etc., but also a good feed for non-herbivorous animals such as pigs, geese, chickens, fishes, etc. The fresh alfalfa has good palatability, high nutrition, rich bioactive substances and good direct feeding effect. However, there are also a number of disadvantages, for example, fresh alfalfa urease can cause ammonia poisoning in ruminants; the contained female hormone 'coumestrol' can cause the animals in the reproductive age group to have reproductive dysfunction; the 'leaf red' containing photosensitive substance can cause 'photosensitive allergy' of livestock and also cause reduction of liver detoxification function and central nervous disorder; the saponin is contained, so that the cattle and the sheep can suffer from acute ruminal tympany due to excessive ingestion, the health is damaged, the production performance is reduced, and the death can be caused by untimely rescue of serious patients.
In order to solve the above problems of fresh alfalfa, to prolong storage and to facilitate circulation, the most widespread method at present is to produce hay after harvesting the alfalfa. However, in addition to the loss of bioactive substances during the production of hay, the leaves of alfalfa and stems are not dried synchronously due to different shapes and different water contents, so that the loss of the leaves is caused, and the nutritional ingredients of the leaves are far higher than those of the stems. Alfalfa hay therefore lags significantly behind fresh alfalfa in palatability, nutritional content, and commercial value.
At present, another solution is to crush grass bodies for ensiling fermentation, but in the whole ensiling fermentation, strains such as saccharomycetes, lactic acid bacteria and the like exist under the same conditions of oxygen, nutrient substances, moisture and temperature, so that the optimum conditions of fermentation temperature, moisture and nutrient cannot be provided for the strains, the controllability of fermentation degree of different strains in a fermentation process and final materials is poor, and the fermentation effect is not ideal.
Disclosure of Invention
The invention aims to provide a preparation method of alfalfa fermentation slurry feed, and provides alfalfa fermentation slurry feed which is rich in nutrition, storage-resistant, good in absorption and free of anti-nutritional factors.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of alfalfa fermentation slurry feed comprises the following steps:
1) crushing and grinding fresh alfalfa, and then heating and sterilizing to obtain sterile alfalfa slurry;
2) the sterile alfalfa slurry enters extrusion separation equipment for solid-liquid separation to obtain dilute liquid and wet slag;
3) the dilute solution is inoculated with mixed bacteria agent 1, glucose and nutrient salt for fermentation;
4) adding the wet residue into a mixed microbial inoculum 2, a complex enzyme preparation, glucose and nutrient salt for fermentation;
5) mixing the fermented materials obtained in the step 3) and the step 4), and grinding uniformly after mixing to obtain the alfalfa fermentation slurry feed.
Preferably, in the step 1), the fresh alfalfa is crushed and ground to 2-10 meshes.
Preferably, in the step 2), the moisture content of wet slag after slurry separation is 40-60%.
Preferably, in the step 3), the number of the lactobacillus strains in the mixed bacterial preparation 1 is: the number of bacillus subtilis strains: the quantitative ratio of the number of bacillus coagulans strains is 100: 0 to 100: 0 to 100.
Preferably, in the step 3), the ratio of dilute liquid: 1, mixing a microbial inoculum: the weight ratio of glucose is 100: 1-10: 2 to 10.
Preferably, in the step 4), the number of the lactobacillus strains in the mixed bacterial agent 2 is: the number of bacillus subtilis strains: bacillus coagulans: the number ratio of the number of the saccharomycete strains is 10000: 0 to 1000: 0 to 1000: 0 to 100.
Preferably, in the step 4), the wet slag: and (3) mixing the microbial inoculum 2: compound enzyme preparation: the weight ratio of glucose is 100: 1-10: 1-10: 2 to 10.
Preferably, in the step 4), the ratio of the activities of the protease, the pectinase and the cellulase in the complex enzyme preparation is 100: 60-80: 60 to 100.
Preferably, in the step 5), the mixed slurry is crushed and ground to 10-100 meshes.
Compared with the prior art, the invention has the beneficial effects that:
the alfalfa is crushed, ground, heated and sterilized, and enters the extrusion separation equipment to obtain the dilute liquid and the wet slag, the dilute liquid has high anaerobic degree, is beneficial to fermentation of probiotics such as lactobacillus and bacillus coagulans, the wet slag is relatively fluffy and rich in oxygen, is suitable for yeast fermentation, and the fermentation temperature and the pH value are more beneficial to the exertion of a complex enzyme preparation. The alfalfa fermentation slurry feed prepared by the invention is rich in nutrients such as proteins, dietary fibers, various sugars, organic acids, probiotics, vitamins, minerals and the like, is storage-resistant and good in absorption, does not contain anti-nutritional factors, and can regulate intestinal tracts.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A preparation method of alfalfa fermentation slurry feed comprises the following steps:
1) crushing 1000 g of fresh alfalfa until the maximum slag particle is not more than 5mm, and then heating and sterilizing to obtain sterile alfalfa slurry;
2) after the sterile alfalfa slurry enters extrusion separation equipment for solid-liquid separation, 500 g of dilute solution and 500 g of wet slag are obtained, and the water content of the wet slag after slurry separation is 50%;
3) the dilute solution is inoculated into 110 g of mixed microbial inoculum, 50 g of glucose and nutrient salt for fermentation for 48h, the number of lactobacillus strains in the mixed microbial inoculum 1 is as follows: the number of bacillus subtilis strains: the number ratio of the bacillus coagulans strain number is 10:1: 5;
4) adding 210 g of mixed microbial inoculum, 10 g of complex enzyme preparation, 30 g of glucose and nutrient salt into the wet residue, fermenting for 48 hours, wherein the number of lactobacillus strains in the mixed microbial inoculum 2 is as follows: the number of bacillus subtilis strains: bacillus coagulans: the number ratio of the number of the saccharomycete strains is 100:5:10:1, and the activity ratio of protease, pectinase and cellulase in the compound enzyme preparation is 10:5: 8;
5) mixing the fermented materials obtained in the steps 3) and 4), grinding uniformly after mixing to ensure that the maximum slag particle is not more than 2mm, and hermetically packaging the mixed materials to obtain the alfalfa fermented slurry feed.
According to the principle that the number of fetuses, the date of milk production and the daily milk yield are similar, 80 healthy Holstein cows in the peak lactation period are randomly divided into 4 groups: a control group, a 4.0 percent (weight ratio) alfalfa grass fermented slurry feed group, a 8.0 percent (weight ratio) alfalfa grass fermented slurry feed group, a 4.0 percent (weight ratio) alfalfa grass whole direct fermented feed group and a 8.0 percent (weight ratio) alfalfa grass whole direct fermented feed group. The pre-test period is 7 days, and the test period is 35 days.
TABLE 1 influence of alfalfa fermented slurry feed on the productivity of Holstein cows at peak lactation
As shown in table 1 above, compared with the control group, after 4.0% and 8.0% alfalfa fermented slurry feeds are added to the concentrate of the dairy cows in the peak lactation period, the milk yield is respectively increased by 10.89% and 16.38%, the milk fat rate is respectively increased by 9.70% and 15.67%, the milk protein rate is increased by 6.21% and 9.48%, the lactose content is respectively increased by 4.55% and 8.28%, the total solid content is respectively increased by 6.98% and 10.82%, and the somatic cell number is respectively decreased by 22.40% and 33.41%.
Compared with the group added with 4.0 percent and 8.0 percent of alfalfa grass whole direct fermentation feed, after 4.0 percent and 8.0 percent of alfalfa grass fermentation slurry feed are added into the dairy cow concentrated feed in the peak lactation period, the milk yield is respectively improved by 9.14 percent and 8.43 percent, the milk fat rate is respectively improved by 6.00 percent and 9.41 percent, the milk protein rate is respectively improved by 2.52 percent and 3.39 percent, the lactose content is respectively improved by 2.64 percent and 2.95 percent, the total solid content is respectively improved by 3.03 percent and 4.08 percent, and the somatic cell number is respectively reduced by 10.58 percent and 15.91 percent.
The results show that: compared with the dairy cattle which are not fed with the alfalfa fermented feed and fed with the whole alfalfa fermented feed, the dairy cattle fed with the alfalfa fermented slurry feed can obviously improve the production performance of the dairy cattle in the lactation peak period, improve the milk yield, improve the content of milk components, reduce the content of somatic cell number in milk, and have obvious economic benefit; and secondly, compared with the feed obtained by directly fermenting the whole piece of alfalfa, the fermented slurry feed obtained by separate fermentation obviously improves the production performance of the dairy cows in the lactation peak period and improves the milk yield.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the present invention as defined in the accompanying claims.
Claims (9)
1. The preparation method of the alfalfa fermentation slurry feed is characterized by comprising the following steps of:
1) crushing and grinding fresh alfalfa, and then heating and sterilizing to obtain sterile alfalfa slurry;
2) the sterile alfalfa slurry enters extrusion separation equipment for solid-liquid separation to obtain dilute liquid and wet slag;
3) the dilute solution is inoculated with mixed bacteria agent 1, glucose and nutrient salt for fermentation;
4) adding the wet residue into a mixed microbial inoculum 2, a complex enzyme preparation, glucose and nutrient salt for fermentation;
5) mixing the fermented materials obtained in the step 3) and the step 4), and grinding uniformly after mixing to obtain the alfalfa fermentation slurry feed.
2. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 1), the fresh alfalfa is crushed and ground to 2-10 meshes.
3. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 2), the moisture content of wet slag after slurry separation is 40-60%.
4. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 3), the number of the lactobacillus strains in the mixed microbial inoculum 1 is as follows: the number of bacillus subtilis strains: the quantitative ratio of the number of bacillus coagulans strains is 100: 0 to 100: 0 to 100.
5. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 3), dilute solution: 1, mixing a microbial inoculum: the weight ratio of glucose is 100: 1-10: 2 to 10.
6. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 4), the number of lactobacillus strains in the mixed microbial inoculum 2 is as follows: the number of bacillus subtilis strains: bacillus coagulans: the number ratio of the number of the saccharomycete strains is 10000: 0 to 1000: 0 to 1000: 0 to 100.
7. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 4), wet slag: and (3) mixing the microbial inoculum 2: compound enzyme preparation: the weight ratio of glucose is 100: 1-10: 1-10: 2 to 10.
8. The method of claim 1 or 7, wherein the method comprises the steps of: in the step 4), the activity ratio of protease, pectinase and cellulase in the compound enzyme preparation is 100: 60-80: 60 to 100.
9. The method for preparing a alfalfa fermentation slurry feed according to claim 1, characterized in that: in the step 5), the mixed slurry is crushed and ground to 10-100 meshes.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614020A (en) * | 2015-12-22 | 2016-06-01 | 河南牧业经济学院 | Micro-ecological alfalfa feed additive for livestock and preparation method thereof |
| CN110468064A (en) * | 2019-04-22 | 2019-11-19 | 河南中微生物科技研究院(有限合伙) | A kind of alfalfa ensilage agent and preparation method thereof |
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2019
- 2019-11-22 CN CN201911152786.8A patent/CN110810624A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105614020A (en) * | 2015-12-22 | 2016-06-01 | 河南牧业经济学院 | Micro-ecological alfalfa feed additive for livestock and preparation method thereof |
| CN110468064A (en) * | 2019-04-22 | 2019-11-19 | 河南中微生物科技研究院(有限合伙) | A kind of alfalfa ensilage agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 刘卢生等: ""紫花苜蓿草渣及浆汁发酵研究"" * |
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