CN101103764A - Method for preparing liquid or semi-solid ferment complete feed containing rich probiotics and polypeptide - Google Patents
Method for preparing liquid or semi-solid ferment complete feed containing rich probiotics and polypeptide Download PDFInfo
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- CN101103764A CN101103764A CNA2007100242763A CN200710024276A CN101103764A CN 101103764 A CN101103764 A CN 101103764A CN A2007100242763 A CNA2007100242763 A CN A2007100242763A CN 200710024276 A CN200710024276 A CN 200710024276A CN 101103764 A CN101103764 A CN 101103764A
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Abstract
The invention relates to a preparation method for a liquid or semi-solid fermentation complete feed with rich probiotic bacteria and peptide, which belongs to the field of bio-fermentation technology. The invention takes animal complete feed, concentrative feed, energy and protein feed as the raw materials, and lactic acid bacteria and zymolytic agent are added, and the water content is adjusted to 50 percent to80 percent, and then the liquid or semi-solid complete feed is made under the temperature control fermentation of 35 DEG C to 37 DEG C for 32 hours to 80 hours. The invention adopts liquid or semi-solid state fermentation, and the process is simple, the cost is low and the control is easy. Finally, the protein in the raw materials of complete feed, concentrative feed or energy and protein feed can be transformed to the micromolecule peptide with biological activity, and has rich probiotic bacteria and peptide. The nutriment of the product which is made by the method is easy to be digested and absorbed by the animal, and helps to the stability of useful intestinal flora of the animal, and has high antioxidant activity, as well as can improve the immunity of the body of the animal, thereby the invention can widely be used in the process for various animal complete feed, concentrative feed and energy and protein raw materials, and is a novel processing method for improving the nutritional quality of feed.
Description
Technical field
Be rich in the liquid state of probio and polypeptide or the preparation method of semi-solid ferment complete feed, belong to technical field of biological fermentation, be specifically related to a kind of method that adopts Lactobacillus plantarum and enzyme fermentation agent production liquid state or semisolid complete feed or calorie-protein feed.
Background technology
Studies have shown that of nutritional physiology in recent years, protein is hydrolyzed into little peptide basically in stomach and small intestine, and then is absorbed by body.The absorption of little peptide has that transport speed is fast, power consumption is low and carrier is difficult for advantages such as saturated, can avoid and the competition of amino acid between absorbing.Studies show that little peptide carrier absorption ability is higher than the summation of various amino acid carrier absorbabilities, therefore, the absorptivity of little peptide mixer is apparently higher than ispol.
Little peptide also can promote the absorption of other nutrients.At first, except mechanism of absorption can promote that amino acid absorbs, little peptide itself was also to the facilitation that is absorbed with of amino acid and residue, as the absorption substrate of enteric cavity, little peptide can not only increase the activity of IBB aminopeptidase and dipeptidase, and can improve the quantity of little peptide carrier.Second, the amino acid residue of little peptide can with metal ion-chelant, avoid that the antagonism factor and other factors of influence directly arrive intestinal brush border to the precipitation or the suction-operated of mineral element in the enteric cavity, and absorbing site generation hydrolysis, thereby increase the absorption of mineral matter element.The 3rd, little Toplink provides complete nitrogen frame for the synthetic fast of body digestive ferment, adds the small peptide material and can significantly improve pepsin, lipase and diastatic activity in feed.In addition, little peptide can preferentially effectively promote the growth of intestinal mucosa tissue as the energy substrate of intestinal mucosa epithelial cell 26S Proteasome Structure and Function growth.Little peptide can be by complete effective absorption, thereby reduces the albumen quality of the end digestion that enters large intestine, reduces the generation of large intestine back segment ammonia and poisonous amine, and alimentary canal is played positive protective effect, keeps the normal immunologic function of alimentary canal.In addition, the little peptide of some physiologically actives, can be directly as neurotransmitter, indirect stimulation enteron aisle hormone receptor or promote the secretion of enzyme and bring into play physiological regulatory action, thereby promote intestinal development.For ruminant, have outside the above characteristic, little peptide can also promote to produce specific digestive ferment microbial growth in the cud, thereby improves the utilize degree of rumen microorganism to roughage.
In the time of with the Production by Microorganism Fermentation polypeptide, inorganic microelements such as selenium, iron, copper can also be converted into organic trace element through thalline, improve trace element utilizing rate, reduce inorganic elements to side effects such as GI stimulations, increase the content of physiological activator such as isoflavones in the tunning.And lactic acid bacteria is the normal flora in the human body intestinal canal, and metabolism can produce several physiological active substances such as organic acid, bacteriocin, has colony balance in the enteron aisle of keeping, and promotes absorption of nutrient ingredients, strengthens the multiple functions such as immunity of body.Lactic acid bacteria and tunning thereof can make livestock and poultry weightening finish increase, survival rate as feed addictive and production efficiency improves, immunity strengthens, and has the effect of preventing and curing diseases.
Utilize the lactobacillus-fermented complete feed and be used in combination protease and discharge the little peptide that albumen contains in the feed, simultaneously, the bitter taste factor in the lactobacillus-fermented energy modified peptides, produce multiple flavor substance, after storage is protected in the product sealing, storage is fragrant more more, has improved the palatability of feed greatly, helps to increase feed intake.Polypeptide nutrition is worth this product and the lactic acid bacteria prebiotic effect combines, and can produce huge economic and social benefit.
Simple to operate with this law liquid state, solid state fermentation, product is feeding animals or carry out simple post processing with long preservation directly, because feed pH value reduces greatly behind the lactobacillus-fermented, antibacterial substances such as organic acids such as lactic acid bacteria, phenyllactic acid and bacteriocin have been produced simultaneously, help suppressing bacterium, mould contamination, improve the security of feed greatly, helped feed storage.
Summary of the invention
The purpose of this invention is to provide a kind of is raw material with complete feed, concentrate feed or calorie-protein feed, produces the method that is rich in active probiotic, polypeptide feed product.
Technical scheme of the present invention: with animal complete feed, feed concentrate feed or calorie-protein feed is raw material, be crushed to the 20-40 order, add lactic acid bacteria and enzyme fermentation agent, regulate water content to 50%～80%, 35～37 ℃ temperature controlled fermentation and obtained liquid state or semi-solid ferment complete feed in 32～80 hours; Technology is:
(1) add lactic acid bacteria: the lactic acid bacteria fermenting agent that is adopted is Lactobacillus plantarum Lp6 (Lactobacillusplantarum Lp6); First fermentation inoculum is 18 hours zymotic fluids of skimmed milk power culture medium of 8%～10%, and later batch of fermentation inoculation is last batch fermentation material or culture purified bacterium liquid again;
(2) add the enzyme fermentation agent: the enzyme that is adopted is selected from a kind of or its combination in acid protease, neutral proteinase or the cellulase;
(3) fermentation condition: regulate water content to 50%～80%, stir, 35～37 ℃ of airtight temperature controlled fermentations 32～80 hours;
(4) finished product is handled: tunning is directly as the feed of feeding animals, or is lower than 50 ℃ of low temperature air blast oven dry and obtains feed dry powder or sealing back long preservation;
The weight of described interpolation enzyme is respectively acid protease 0.1%～2% in the weight of raw material feed dry weight, neutral proteinase 0.05%～0.5% and/or cellulase 0.05%～0.5%.
The bacterium liquid that connects by the percentage of culture of strains basic weight amount in complete feed butt weight, the inoculum concentration of controlling first Lactobacillus plantarum is 0.2%～1% of a complete feed butt weight, later batch the fermentation inoculum concentration be 3%～5% of fresh fermentation material.
Raw materials used is all kinds beasts, birds and aquatic products complete feed, concentrate feed or calorie-protein feed.
The fermentation animal complete feed of gained of the present invention shows that after measured water-solubility protein accounts for 39%～56% of feed crude protein, and lactic acid content is 5%～6%, and pH is 3.8～4.5, and the viable lactic acid bacteria number is 10
7More than.
Utilize the resulting fermentation of the present invention animal complete feed, concentrate feed or calorie-protein feed, can be used for various domestic animal daily rations, fishes and shrimps daily ration or other cultivated animals daily ration.Used livestock and poultry of the present invention, aquatic products complete feed are that piglet opens the food complete feed, the fattening of pig material, and various full price feed for layer chicken, various meat-chicken complete feedstuffs, high yield cow replenishes material, various aquatic products complete feeds, concentrate feed and feedstuff etc.
Sample water dissolubility crude protein assay method:
Main agents: 1. the ninhydrin developer (after the 2g ninhydrin is dissolved in the 50ml EGME, adds 25mL pH5.5,0.2M acetate buffer, 25mL deionized water, 0.08mmol/L SnCl
22H
2O); 2. 50% (V/V) ethanolic solution; 3. glycine.All reagent is AR reagent.
Key instrument: 721 spectrophotometers, pH meter magnetic stirring apparatus, semi-automatic azotometer etc.
The drafting of calibration curve: get and be settled to 100mL after the dry glycine of crossing of 0.1000g is dissolved in distilled water, take out the 2.00mL constant volume, obtain the solution of 20mg/L to 100mL.Getting this solution again, to be diluted to content be the drafting that the solution of 2-20 μ g/mL is used for calibration curve.Get 0.5mL and measure, in test tube, add the 2.00mL developer, heat 30min behind the mixing in the boiling water bath with dilution, cold water cooling then, the ethanolic solution mixing of adding 6.00mL 50% is placed 15min, does blank test simultaneously.Return to zero in the mensuration A of 570nm place value with the blank pipe with the 1cm cuvette.
The preparation of sample extracting solution: get the forward and backward sample 1g of fermentation, the ratio that is suspended in sample and water is that 1: 40 (calculating with the sample dry weight) adds entry, 60 ℃ of water-bath effect 2.5h, and the centrifugal 20min of 5000rpm/min obtains sample extracting solution.
In the sample extracting solution-NH
2Determination on content: sample thief extract 0.2-0.5mL is settled to 50mL, get the 0.4mL dilution in test tube and add 1.60mL distilled water and 1.00mL developer mixing and be placed on and heat 15min in the boiling water bath, do blank test simultaneously, operate same calibration curve later on.Utilize in the calibration curve calculation sample extract-NH
2Content (mmol/g).
The calculating of sample water dissolubility crude protein
The present invention compared with prior art has following characteristics and beneficial effect:
1. the present invention adopts liquid state or semi-solid ferment zymolysis technique, have that production technology is simple, controllability good, water saving, energy-conservation, save labour, equipment investment few, be easy to advantage such as suitability for industrialized production.
2. the inventive method makes and is rich in little peptide and active probiotic beasts, birds and aquatic products complete feed or raw material, has to strengthen the function that albumen and mineral element and other nutrients absorb, provide intestinal beneficial bacterium.
3. adopt lactic acid bacteria and enzyme fermentation agent combined ferment complete feed, concentrate feed or raw material, can make not only that high molecular PD is little peptide in the feed, also can eliminate raw material such as dregs of beans contains in the feed protease inhibitor and flatulence factor etc. the disadvantageous composition of livestock birds health.
4. the present invention adopts microbial fermentation, produces flavor substance and bacteriocins such as multiple organic acid, ester class during the fermentation, helps to improve feed intake and feed and preserves, the prevention pathogenic bacterial infection.
5. fermentation produces physiological activators such as isoflavones and phenols, and the various active peptide can improve the blood plasma glutathione content, improve activities of antioxidant enzymes such as hepatocuprein and glutathione peroxidase, and strengthen bioactive functions such as immunity of organisms.
6. improve animal growth rate, improve feed efficiency.Can increase daily gain 6% and improve food conversion ratio 5%～9%.
The specific embodiment
1. raw material pulverizing preliminary treatment: be crushed to the 20-40 order, complete diet pellet, concentrate feed are according to various its feeding standard feed prepared, aborning, spot-check mensuration to the nutritional labeling of the raw material of different batches.So that reasonably select the technological parameter such as addition, inoculum concentration of water.
2. strain preparation: producing bacterial classification is Lactobacillus plantarum Lp6 (Lactobacillus plantarum Lp6), adopts 8%～10% skimmed milk power culture medium, and 37 ℃ of static cultivation 18h obtain fermentation seed.
3. add enzyme and inoculation Lactobacillus plantarum: the enzyme that is adopted is selected from a kind of or its combination in acid protease, neutral proteinase or the cellulase.The good lactobacillus plantarum ferment of while inoculated and cultured.
4. temperature controlled fermentation: regulate water content to 50%～80%, 37 ℃ airtight temperature controlled fermentation 32～80 hours.
5. finished product is handled: the salable preservation of tunning, and directly use in a short time, as want long preservation, can dry to water content in the low temperature air blast that is lower than 50 ℃ and be lower than 12%.
The present invention adds the kind and the enzyme amount of enzyme, bacterial classification and raw material proportioning, and fermentation temperature, isoparametric selection of time are referring to the following example.
Embodiment 1:
Preparation weanling pig fattening pig complete feed 100Kg, add acid protease 0.8%, inoculating lactic acid bacterium liquid 0.5%, transfer water content to 50% with 40 ℃ of running water, 35～37 ℃ of airtight packages fermented 36 hours, the pH of final products is 4.1, and fermentation back soluble protein is about 182% before the fermentation, and packing is preserved or directly used.
Embodiment 2: get fattening pig complete feed 100Kg, add acid protease 0.4%, inoculating lactic acid bacterium liquid 0.2%, transfer water content to 50% with 40 ℃ of running water, 35～37 ℃ of sealed fermentings 36 hours, the pH of final products is 4.0, and fermentation back soluble protein is about 142% before the fermentation, and packing is preserved or directly used.
Embodiment 3: preparation weanling pig concentrate feed 100Kg, add acid protease 1.0%, inoculating lactic acid bacterium liquid 0.5%, transfer water content to 50% with 40 ℃ of running water, 35～37 ℃ of airtight packages fermented 60 hours, the pH of final products is 4.2, and fermentation back solubility crude protein is about 152% before the fermentation, preserves or directly mixes other feeds and use.
Embodiment 4:100Kg dish dregs of rice powder, add acid protease 0.5%, cellulase 0.05% and neutral proteinase 0.05%, inoculating lactic acid bacterium liquid 0.4%, transfer water content to 80% with 40 ℃ of running water, 35～37 ℃ of airtight packages fermented 72 hours, the pH of final products is 3.8, and fermentation back solubility crude protein is about 152% before the fermentation, and packing is preserved or directly used.
Claims (2)
1. one kind is rich in the liquid state of probio and polypeptide or the preparation method of semi-solid ferment complete feed, it is characterized in that with animal complete feed, feed concentrate feed or calorie-protein feed be raw material, be crushed to the 20-40 order, add lactic acid bacteria and enzyme fermentation agent, regulate water content to 50%～80%, 35～37 ℃ temperature controlled fermentation and obtained liquid state or semi-solid ferment complete feed in 32～80 hours; Technology is:
(1) add lactic acid bacteria: the lactic acid bacteria fermenting agent that is adopted is Lactobacillus plantarum Lp6 (Lactobacillusplantarum Lp6); First fermentation inoculum is 18 hours zymotic fluids of skimmed milk power culture medium of 8%～10%, and later batch of fermentation inoculation is last batch fermentation material or culture purified bacterium liquid again;
(2) add the enzyme fermentation agent: the enzyme that is adopted is selected from a kind of or its combination in acid protease, neutral proteinase or the cellulase;
(3) fermentation condition: regulate water content to 50%～80%, stir, 35～37 ℃ of airtight temperature controlled fermentations 32～80 hours;
(4) finished product is handled: tunning is directly as the feed of feeding animals, or is lower than 50 ℃ of low temperature air blast oven dry and obtains feed dry powder, or sealing back long preservation;
The weight of described interpolation enzyme is respectively acid protease 0.1%～2% in the weight of raw material feed dry weight, neutral proteinase 0.05%～0.5% and/or cellulase 0.05%～0.5%;
The bacterium liquid that connects by the percentage of culture of strains basic weight amount in complete feed butt weight, the inoculum concentration of controlling first Lactobacillus plantarum is 0.2%～1% of a complete feed butt weight, later batch the fermentation inoculum concentration be 3%～5% of fresh fermentation material.
2. preparation method according to claim 1 is characterized in that raw materials used be all kinds beasts, birds and aquatic products complete feed, concentrate feed or calorie-protein feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100242763A CN101103764A (en) | 2007-07-27 | 2007-07-27 | Method for preparing liquid or semi-solid ferment complete feed containing rich probiotics and polypeptide |
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| CNA2007100242763A CN101103764A (en) | 2007-07-27 | 2007-07-27 | Method for preparing liquid or semi-solid ferment complete feed containing rich probiotics and polypeptide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
| CN104957373A (en) * | 2015-06-10 | 2015-10-07 | 山东省农业科学院畜牧兽医研究所 | Method for reducing heavy metal emission in livestock and poultry manure |
| CN105231000A (en) * | 2015-09-25 | 2016-01-13 | 广东海洋大学 | Method for producing compound antibacterial nutrient feed additive by utilizing offal of aquatic products |
| CN105533140A (en) * | 2015-12-05 | 2016-05-04 | 吉林省农业科学院 | Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal |
| CN108546725A (en) * | 2018-01-23 | 2018-09-18 | 北京联合大学 | A kind of biologically active peptide and preparation method thereof prepared using horse blood |
| CN110050886A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of dry and wet mixing complete feed of long shelf-life and preparation method thereof |
| CN110063420A (en) * | 2019-05-28 | 2019-07-30 | 安徽天邦饲料科技有限公司 | A kind of sucking pig liquid nutritional milk and preparation method thereof |
-
2007
- 2007-07-27 CN CNA2007100242763A patent/CN101103764A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
| CN104957373A (en) * | 2015-06-10 | 2015-10-07 | 山东省农业科学院畜牧兽医研究所 | Method for reducing heavy metal emission in livestock and poultry manure |
| CN104957373B (en) * | 2015-06-10 | 2018-07-24 | 山东省农业科学院畜牧兽医研究所 | The preparation method of the feed of heavy metals emission in a kind of reduction feces of livestock and poultry |
| CN105231000A (en) * | 2015-09-25 | 2016-01-13 | 广东海洋大学 | Method for producing compound antibacterial nutrient feed additive by utilizing offal of aquatic products |
| CN105231000B (en) * | 2015-09-25 | 2020-01-07 | 广东海洋大学 | Method for producing antibacterial nutritional compound feed additive by using aquatic product leftovers |
| CN105533140A (en) * | 2015-12-05 | 2016-05-04 | 吉林省农业科学院 | Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal |
| CN108546725A (en) * | 2018-01-23 | 2018-09-18 | 北京联合大学 | A kind of biologically active peptide and preparation method thereof prepared using horse blood |
| CN108546725B (en) * | 2018-01-23 | 2021-08-27 | 北京联合大学 | Bioactive peptide prepared from horse blood and preparation method thereof |
| CN110050886A (en) * | 2019-05-15 | 2019-07-26 | 北京波尔莱特饲料有限公司 | A kind of dry and wet mixing complete feed of long shelf-life and preparation method thereof |
| CN110063420A (en) * | 2019-05-28 | 2019-07-30 | 安徽天邦饲料科技有限公司 | A kind of sucking pig liquid nutritional milk and preparation method thereof |
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Open date: 20080116 |