CN103385350B - Soft particle milk replacer containing high fructose corn syrup and preparation thereof - Google Patents
Soft particle milk replacer containing high fructose corn syrup and preparation thereof Download PDFInfo
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Abstract
The invention discloses a soft particle milk replacer containing a high fructose corn syrup, belonging to the technical field of a feed formula design and feed processing. The soft particle milk replacer is prepared by the following steps of: taking a compound fermented soybean meal which is prepared by sequentially mixing bacillus subtilis and a pickle fermentation solution to ferment, and rice as main materials; adding steam fish meal, flour, a peeled soybean meal and the like for match; carrying out ultrafine crushing on the materials and preparing particle materials by using a soft granulator; uniformly spraying a composite syrup prepared from dissolvable components including emulsified fat powder, whey powder, milk powder, white granulated sugar, mycose, soyabean lecithin powder, calcium formate and the like to the surface of the particle materials by using a syrup spraying and dripping device. The nutrition of a product is fully balanced and the nutrition is complete; the soft particle milk replacer is easy to digest and absorb after multiple times of fermentation; the quality is stable; a process of combining a soft granulation system with a syrup spraying and dripping system is adopted so that the occurrence that particles become hard in a storage process of the particle feed is reduced; compared with a dry particle feed, the soft particle milk replacer has the advantages that the loss of the nutrition in a granulation heating and drying process is avoided, and the good palatability of the soft particle feed can also be kept.
Description
Technical field
The invention belongs to Feeding Prescription Design and feed processing technology field, relate to particularly a kind of formula Design and processing technology of the soft particle creep feed that contains HFCS.
Background technology
Current, Chinese feed processing technology has been obtained very much progress.With regard to feed physical aspect, develop into commercialization powder from peasant household's swill fodder, develop into commercialization pellet from commercialization powder, then develop into sprayed particle feed after commercialization liquid.
But newborn piglet palatability and feed intake remain the bottleneck of product research.Research shows, newborn piglet is more liked the liquid feed that approaches breast milk, but liquid feed is difficult for storage, and easily microbiological contamination is rotten.The particulate material that existing market is generally used, because primary raw material contains a large amount of starch, in storage process, inevitably there is the problem of aging of starch, thereby cause slightly long products start to become fragile hardening, mouthfeel variation of storage time, thereby affect the palatability of product, reduced the feed intake of newborn piglet.Particulate material, in process, because high-temperature heating is granulated, causes the loss of thermal sensitivity nutriment in addition, reduces feed value, thereby affects breeding performonce fo animals.
Application number is 201110394060.2 Chinese invention patent " a kind of process for solid state fermentation of high digestibility fermented bean dregs ", this invention relates to a kind of process for solid state fermentation of high digestibility fermented bean dregs, comprise that bacterial classification liquid fermentation, bacterium liquid mix with dregs of beans, fermentation vat accumulation, cover film, solid state fermentation, oven dry, pulverizing and packaging step, above-mentioned bacterial classification liquid fermented bacterium weight ratio is bacillus: lactic acid bacteria: saccharomycete=1.8-2.2:0.8-1.2:0.8-1.2; Bacterium liquid mixes with dregs of beans and front bacterium liquid is carried out to hyperthermic treatment, and the temperature of hyperthermic treatment is 34-36 DEG C; Bacterium liquid mixes employing Horizontal type helical ribbon mixer, incorporation time 3-5min with dregs of beans; Fermentation vat piling height is 40-60cm; Solid state fermentation time 3.5-5 days.Application number is China's invention " being rich in the production technology of the fermented bean dregs of limiting amino acid " of 201110286768.6, relates to a kind of production technology of fermented bean dregs.This invention is that will to solve in the fermented bean dregs of existing explained hereafter the content of restricted amino-lysine and methionine low, with after its feeding cow, and the problem that the output of milk of milk cow is on the low side.Method: dregs of beans powder and corn protein powder are mixed, add water, obtain mixed solution; Prepare fermentation medium; Add protease and trypsase, enzymolysis; After enzymolysis, fermentation medium is heated to sterilizing; Bafillus natto is inoculated in fermentation medium and is cultivated, and sterilizing obtains bacillus natto to ferment liquid; In bacillus natto to ferment liquid, add K2SO4, the bright string of inoculation goldbeater's skin coccus, cultivates, and tunning is dry, obtains fermented bean dregs.The total protein content of the fermented bean dregs of this invention can reach 56.8%, and methionine content can reach 43.56mg/g, and lysine content can reach 74.872mg/g.Produce for protein feed.Application number is China's invention " a kind of anaerobic fermentation soya bean watse and production method thereof " of 200610013969.8, relate to the survival rate of thermally labile ANFs and thermally-stabilised class ANFs in anaerobic fermentation soya bean watse and be less than 5%, in anaerobic fermentation soya bean watse protein molecular weight at the little peptide of 19-48KD higher than 90%, its preparation method is for pulverizing and sieving, spray zymotic fluid, sealing and fermenting, directly feeding or oven dry encapsulation, and alternative plasma protein, the animal-based protein matter feedstuffs such as fish meal, can partly substitute organic acid, all or part of substitute antibiotics, superiority is without sterilization treatment, anaerobic fermentation, the inactivation ratio of all kinds of ANFs is high, good stability, success rate is high, fanning economics is high.Application number is China's invention " preparation method of high bioactivity fermented bean dregs " of 201210341369.X, disclose a kind of preparation method of high bioactivity fermented bean dregs, this preparation method comprises: prepare that composite zymocyte, dregs of beans inoculation mixing, solid state fermentation, fermented bean dregs material part are dry, dry fermented bean dregs and original fermented bean dregs mixes, packaging.Because fermented bean dregs for this reason does not pass through the process of high temperature drying, avoid the destruction of dry run so can make to greatest extent nutritional labeling in fermented bean dregs, after packaging, fermentation probio substantially all survives and can preserve for a long time, and material nutritive value and biologically active are very high.Application number is China's invention " a kind of production technology that is rich in biotin fermented bean dregs " of 201210376680.8, discloses a kind of production technology that is rich in biotin fermented bean dregs, comprises the following steps: prepared by one-level kind; Prepared by secondary kind; Prepared by three grades of kinds; Solid fermentation; Dry and pulverize.The production of part biological element of the present invention is prepared one time fermentation with production by kind and is completed, and has saved resource and cost, and whole process is pollution-free; In fermentation finished product, biotin content is significantly improved before fermenting, and the Soybean Anti-nutritional Factors such as phytic acid are fully degraded, and each nutritional labeling improves as albumen, little peptide, amino acid etc. all have in various degree.Application number is China's invention " a kind of method of dregs of beans and fermented bean dregs processing feed " of 201110227686.4, discloses a kind of method of utilizing the feature of fermented bean dregs to make the feed that is applicable to animal and livestock and poultry.Fermented bean dregs has the following advantages: improved the solubility of dregs of beans albumen, be beneficial to digestion; Reduced the molecular weight of albumen in dregs of beans, a part has wherein reached even amino acid levels of little peptide level, can directly be absorbed by animal; Reduce ANFs, be beneficial to digestion and attract, have certain aromatic odor and delicate flavour, have certain food calling effect, palatability is better.
Foregoing invention all adopts after bean pulp fermentation, prepare feed, ferment and can remove the ANFs material in dregs of beans by microorganism, improve the solubility of dregs of beans albumen, improve the organic acid in fermented bean dregs, macro-molecular protein in dregs of beans is converted into the micromolecule polypeptide that is more conducive to digest and assimilate, thereby improves the feeding value of dregs of beans.But foregoing invention is dried after still generally adopting and fermenting or the method for the dry rear packaging of part, although bean pulp fermentation is from nutritive value and absorb angle, there is marked improvement, but due to the restriction of moulding process, can not fundamentally solve the become fragile problem of hardening, mouthfeel variation of storage life product.Particulate material, in process, because high-temperature heating is granulated, easily causes the loss of thermal sensitivity nutriment in addition, reduces feed value, thereby affects breeding performonce fo animals.
Therefore, for the deficiencies in the prior art, need badly and provide one can reduce the sclerosis of particulate material storage process, reduce the loss of nutriment in high temperature granulating process, have again good attractant, palatability, improve the feed of newborn piglet feed intake simultaneously.
Summary of the invention
The problem such as the hardening that becomes fragile, mouthfeel variation that the present invention seeks to occur in storage process for conventional particles material, to there is better retentiveness, and protect the raw materials such as soft and emulsibility and carry out reasonably combined, reduce by soft granulating system the loss that thermal sensitivity nutriment in product comprises vitamin, enzyme preparation and microorganism formulation, attractant by the further raising product of arranging of molasses add-on system, protect soft and nutrition full price.In order to address the above problem, the invention provides a kind of soft particle creep feed that contains HFCS, comprise the raw material composition of following parts by weight:
Rice 50-60 part, steam fish meal 2-4 part, flour 3-5 part, dehulled soybean meal 2-4 part, chyle fat powder 1-2 part, whey powder 5-10 part, milk powder 3-5 part, white granulated sugar 1-2 part, trehalose 0.5-1 part, HFCS 0.5-1 part, composite fermentation dregs of beans 10-20 part, glucose 2-3 part, soybean lecithin powder 1-2 part, salt 0.3-0.5 part, calcium dihydrogen phosphate 0.8-1.2 part, calcium formate 0.01-0.02 part, amino acid 0.2-0.5 part, complex enzyme 0.05-0.1 part, B B-complex 0.3-0.4 part, composite trace element 0.2-0.6 part;
Described soybean lecithin powder, is a kind of naturally occurring emulsifying agent, and high-energy trophism raw material provides the necessary phosphatide of animal body, choline, has the function of obvious promotion growth, can prevent particle sclerosis simultaneously.
Chyle fat powder is selected Canon or other commercially available prod.
Described whey powder is the preparation of roller drying technique, and wherein lactose is beta lactose (sugariness exceedes alpha-lactose);
Described complex enzyme consists of: protease 1-3 part, amylase 2-5 part.
Described amino acid masses ratio is Dlys:Dmet:Dthr:Dtrp:Dval=100:35:63:21:69.
Described B B-complex composition is vitamin A 1-3 part, vitamin D 2-5 part, vitamin E 3-6 part.
Described composite trace element consists of: ferrous sulfate 1-3 part, manganese sulfate 2-4 part, zinc oxide 1-2 part, basic copper chloride 3-6 part, copper sulphate 1-3 part, sodium selenite 1-3 part, cobalt chloride 1-3 part, calcium iodate 2-3 part.
The preparation method of described composite fermentation dregs of beans is as follows:
Dregs of beans is pulverized to obtain to dregs of beans powder, by bean cake powder and corn flour in mass ratio 15:2-4 mix, obtain mix powder, in mix powder, add 5-8 times of water gaging and control temperature to 50-70 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, makes the pH value of mixture be controlled at 5.5-6.5 in enzymolysis process, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate, 0.5% sodium sulphate, then in 110-121 DEG C of moist heat sterilization 15-20min; In fermentation medium, add mixing bacillus subtilis seed liquor, inoculum concentration is 4-8%, temperature 25-30 DEG C, under the condition that pH value is 5-7, cultivate the pickled vegetable fermentation liquor that adds hydrolysed mix quality 20-35% after 30-34h after holding temperature keep 8-15 hour at 10-18 DEG C.Then the dry composite fermentation bean cake powder that obtains.
Described acid protease addition is the 0.2-1% of dregs of beans powder quality, and AMS is the 0.01-0.05% of dregs of beans powder quality, and described trypsase is the 0.4-1% of dregs of beans powder quality;
Bacillus subtilis (Bacillus subtilis) bacterial classification is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:1-3; CICC10063 and CICC20030 seed liquor are mixed according to volume ratio after cultivating respectively.
CICC20030 culture medium composition: peptone 5.0g, beef leaching thing 3.0g, NaCl5.0g, agar 15.0g, distilled water 1.0L, 3mg MnSO4H2O, pH7.0.Bacillus subtilis CICC20030 produces the energetic bacterial strain of alkali protease.
CICC10063 culture medium composition: NaCl5.0g, beef extract 10.0g, dregs of beans 20g, peptone 10.0g, distilled water 1.0L, pH7.2.CICC10063 produces protease.
Described pickled vegetable fermentation liquor is the fermented liquid of removing pickles gained after pickle fermentation; Pickled vegetable fermentation liquor is for obtaining or adopt lactic acid bacteria, saccharomycete or its mixed bacteria to prepare through the old liquid fermentation of traditional pickles.
Pickled vegetable fermentation liquor also can adopt the method that is prepared as follows to obtain:
Vegetable cleaning, cutting, puts into pickle production container, adds subsequently fermenting agent, the 0.5-2% sucrose of 0.01-1 g/kg, the salinity of 0.5-2%, airtight container; Control temperature and carry out the prior fermentation of 15-30 hour at 15-30 DEG C, control subsequently temperature and carry out after fermentation in 10-30 hour at 10-25 DEG C; Seal of vessel between whole yeast phase; After vegetables are removed in the complete separation of fermenting, remaining liq is pickled vegetable fermentation liquor.
Described fermenting agent is made up of lactic acid bacteria, saccharomycete or the two.
The preparation method of feed of the present invention: the resolvability components such as chyle fat powder, whey powder, milk powder, white granulated sugar, trehalose, soybean lecithin powder and calcium formate, by after proportioning feeding, are dissolved in HFCS, make composite syrup;
By rice, dehulled soybean meal and composite fermentation dregs of beans carry out after by proportioning feeding just pulverizing; Ultramicro grinding is to mix after 15 μ m together with other raw materials again, mixture homogeneity≤7%; Send into again soft granulator and granulate, pulverization rate≤6%;
Utilize syrup spray drip apparatus composite syrup evenly to be sprayed to the surface of particulate material, particle diameter 1-3mm.Finally be packaged as finished product by the weighed amount of automatic packaging.
Beneficial effect:
Adopt soft granulating system to drip with syrup spray the technique that system is combined, the raw material that HFCS etc. can obviously be improved to feed mouthfeel is sprayed on particulate material surface, improved feed palatability, protect soft and attractant, reduce the generation of particle hardening in pellet storage process, soft granulation has reduced the loss of nutritional labeling in granulate heating and dry run, improves the water-retaining property of product, constant product quality, nutrition full price balance, so also can keep soft pellet diet good palatability.Adopt trehalose to replace part white granulated sugar, can effectively prevent regrouping of starch chain, delay the aging of starch, reduce the risk that sclerosis easily occurs in product storage process.Trehalose also has special oxygenate structure in addition, has good lock water function, can effectively prevent scattering and disappearing of moisture in food, can effectively prevent the prolongation gradually generation of hardening of particulate material along with period of storage for feedstuff industry.Glucose can directly absorb in addition, has both improved palatability, does not increase again animal stomach burden.
Protease can be in suitable Water Under solution feed plant and animal proteinoid, become peptide and free amino acid, can improve the utilization ratio of animal to protein, promote the absorption of animal to protein in feed, and then promote the growing of animal, especially growing animal.External source amylase helps lend some impetus to early weaned piglets Fast Growth, and can reduce enteron aisle far-end and do not digest the adverse effect that chyme produces, thereby reduces weaning brash rate.
After mixing with corn flour, dregs of beans adopts two strain fermentations, corn has made up the deficiency of carbon source in dregs of beans, particularly rich bacterial classification mixed processing of producing protease, effectively improve the decomposition efficiency of dregs of beans, the total protein content of composite fermentation dregs of beans can reach 56.8%, methionine content can reach 43.56mg/g, and lysine content can reach 74.872mg/g.The small peptide component that in composite fermentation dregs of beans protein degradation products, molecular weight is less than 1000Da accounts for more than 92%.
In composite fermentation dregs of beans, add pickled vegetable fermentation liquor and carry out cold fermentation processing, after can making to ferment, in dregs of beans, be rich in lactic acid bacteria, and the nutriments such as the vegetables vitamin of enrichment in pickled vegetable fermentation liquor also enter dregs of beans, the pickled vegetable fermentation liquor that is rich in lactic acid has effectively increased the palatability of feed product, and more nutrition is more agreeable to the taste to make composite fermentation dregs of beans.
Detailed description of the invention
Below by specific embodiment narration product composition of the present invention and technique.Unless stated otherwise, in the present invention, technological means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope, the various changes that technical scheme in these embodiments is carried out or change also belong to protection scope of the present invention.
Embodiment 1
(1) a soft particle creep feed that contains HFCS, comprises that the raw material of following parts by weight forms:
50 parts, rice, 2 parts of steam fish meal, 3 parts, flour, 4 parts of dehulled soybean meals, 2 parts, chyle fat powder, 5 parts of whey powders, 5 parts of milk powder, 2 parts of white granulated sugars, 1 part of trehalose, 1 part of HFCS, 10 parts of composite fermentation dregs of beans, 3 parts of glucose, 2 parts, soybean lecithin powder, 0.4 part of salt, 0.8 part of calcium dihydrogen phosphate, 0.01 part of calcium formate, 0.5 part, amino acid, 0.05 part of complex enzyme, 0.4 part of B B-complex, 0.6 part of composite trace element;
Described soybean lecithin powder, is a kind of naturally occurring emulsifying agent, high-energy trophism raw material, provide animal body must phosphatide, choline, have the function of obvious promotion growth, can prevent particle sclerosis simultaneously.
Chyle fat powder is selected Canon or other commercially available prod.
Described whey powder is the preparation of roller drying technique, and wherein lactose is beta lactose.
Described complex enzyme consists of: 2 parts, protease, 4 parts of amylase.
Described amino acid masses ratio is Dlys:Dmet:Dthr:Dtrp:Dval=100:35:63:21:69.
Described B B-complex composition is 2 parts of vitamin As, 5 parts of vitamin Ds, 4 parts of vitamin Es.
Described composite trace element consists of: 4 parts of 3 parts of manganese sulfates of ferrous sulfate, 1 part, zinc oxide, 6 parts of basic copper chlorides, 2 parts, copper sulphate, 3 parts of sodium selenites, 1 part of cobalt chloride, 2 parts of calcium iodates.
The preparation method of described composite fermentation dregs of beans is as follows:
Dregs of beans is pulverized to obtain to dregs of beans powder, by dregs of beans powder and corn protein powder in mass ratio 15:3 mix, obtain mix powder, in mix powder, add 6 times of water gagings and control temperature to 60 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, makes the pH value of fermentation medium be controlled at 5.5 in enzymolysis process, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate and 0.5% sodium sulphate, then in 121 DEG C of moist heat sterilization 20min; To adding and mix bacillus subtilis seed liquor in fermentation medium, inoculum concentration is 5%, under the condition that 25 DEG C of temperature, pH value are 6, cultivates after 32h, adds the pickles of the hydrolysed mix quality 25% dry composite fermentation bean cake powder that obtains of then spraying.
Zymotic fluid, holding temperature keeps 8 hours at 15 DEG C.Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:1; CICC10063 and CICC20030 seed liquor are mixed according to volume ratio after cultivating respectively.
CICC20030 culture medium composition: peptone 5.0g, beef leaching thing 3.0g, NaCl5.0g, agar 15.0g, distilled water 1.0L, 3mg MnSO4H2O, pH7.0.Bacillus subtilis CICC20030 produces the energetic bacterial strain of alkali protease.
CICC10063 culture medium composition: NaCl5.0g, beef extract 10.0g, dregs of beans 20g, peptone 10.0g, distilled water 1.0L, pH7.2.CICC10063 produces protease.
Described acid protease addition is 0.6% of dregs of beans powder quality, and AMS is 0.03% of bean cake powder quality, and described trypsase is 0.7% of dregs of beans powder quality.
The preparation of bacillus subtilis seed liquor:
(1) first order seed is cultivated: bacillus subtilis slant strains is accessed in 500 ml shake flasks to 100 milliliters of culture medium loading amounts, 180 revs/min of rotary shaking tables, 30 DEG C of cultivation temperature, incubation time 24 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum concentration access of 10%, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of culture medium loading amounts, 100 revs/min of rotary shaking tables, 30 DEG C of cultivation temperature, incubation time 24 hours with 10% inoculum concentration;
(4) first class seed pot is cultivated: the first class seed pot by three grades of seeds taking 10% inoculum concentration access total measurement (volume) as 150L, fermentation medium loading amount 100L, 28 DEG C of cultivation temperature, 100 revs/min of mixing speeds, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
Pickle preparation method is with invention " fermented preparation method of vegetables ", and number of patent application is described in 201010247425.4, and according to the method preparation, pickles separate and obtain pickled vegetable fermentation liquor.
(2) preparation method: the resolvability components such as chyle fat powder, whey powder, milk powder, white granulated sugar, trehalose, soybean lecithin powder and calcium formate, by after proportioning feeding, are dissolved in HFCS, make composite syrup;
By rice, dehulled soybean meal and composite fermentation dregs of beans carry out after by proportioning feeding just pulverizing; Ultramicro grinding is to mix after 15 μ m together with other raw materials again, mixture homogeneity≤7%; Send into again soft granulator and granulate, pulverization rate≤6%;
Utilize syrup spray drip apparatus composite syrup evenly to be sprayed to the surface of particulate material, particle diameter 1mm.Finally be packaged as finished product by the weighed amount of automatic packaging.
Embodiment 2
(1) a soft particle creep feed that contains HFCS, comprises that the raw material of following parts by weight forms:
55 parts, rice, 3 parts of steam fish meal, 4 parts, flour, 3 parts of dehulled soybean meals, 1 part, chyle fat powder, 8 parts of whey powders, 4 parts of milk powder, 1 part of white granulated sugar, 0.5 part of trehalose, 1 part of HFCS, 15 parts of composite fermentation dregs of beans, 2 parts of glucose, 2 parts, soybean lecithin powder, 0.4 part of salt, 1.0 parts of calcium dihydrogen phosphates, 0.15 part of calcium formate, 0.3 part, amino acid, 0.1 part of complex enzyme, 0.4 part of B B-complex, 0.4 part of composite trace element;
Chyle fat powder is selected Canon or other commercially available prod.
Described whey powder is the preparation of roller drying technique, and wherein lactose is beta lactose;
Described complex enzyme consists of: 2 parts, protease, 5 parts of amylase.
Described amino acid masses ratio is Dlys:Dmet:Dthr:Dtrp:Dval=100:35:63:21:69.
Described B B-complex composition is dehydroretinol part, neo dohyfral D3 part, 4 parts of vitamin Es.
Described composite trace element consists of: 2 parts, ferrous sulfate, 3 parts of manganese sulfates, 1 part, zinc oxide, 5 parts of basic copper chlorides, 2 parts, copper sulphate, 2 parts of sodium selenites, 2 parts of cobalt chlorides, 2 parts of calcium iodates.
The preparation method of described composite fermentation dregs of beans is as follows:
Dregs of beans is pulverized to obtain to dregs of beans powder, by dregs of beans powder and corn protein powder in mass ratio 15:3 mix, obtain mix powder, in mix powder, add 6 times of water gagings and control temperature and arrive and 60 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, makes the pH value of mixture be controlled at 6.0 in enzymolysis process, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate, 0.5% sodium sulphate, then in 121 DEG C of moist heat sterilization 15min; In fermentation medium, add mixing bacillus subtilis seed liquor, inoculum concentration is 6%, and 28 DEG C of temperature, under the condition that pH value is 6, are cultivated after 32h, add after the pickled vegetable fermentation liquor of hydrolysed mix quality 30%, and holding temperature keeps 12 hours at 15 DEG C.Then the dry composite fermentation bean cake powder that obtains.
Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:2; CICC10063 and CICC20030 seed liquor are mixed according to volume ratio after cultivating respectively.
CICC20030 culture medium composition: peptone 5.0g, beef leaching thing 3.0g, NaCl5.0g, agar 15.0g, distilled water 1.0L, 3mg MnSO4H2O, pH7.0.Bacillus subtilis CICC20030 produces the energetic bacterial strain of alkali protease.
CICC10063 culture medium composition: NaCl5.0g, beef extract 10.0g, dregs of beans 20g, peptone 10.0g, distilled water 1.0L, pH7.2.CICC10063 produces protease.
Described acid protease addition is 0.2% of dregs of beans powder quality, and AMS is 0.01% of bean cake powder quality, and described trypsase is 0.9% of dregs of beans powder quality.
Pickled vegetable fermentation liquor, for adopting traditional zymotic method made pickles, separates and obtains pickled vegetable fermentation liquor.
(2) preparation method: the resolvability components such as chyle fat powder, whey powder, milk powder, white granulated sugar, trehalose, soybean lecithin powder and calcium formate, by after proportioning feeding, are dissolved in HFCS, make composite syrup;
By rice, dehulled soybean meal and composite fermentation dregs of beans carry out after by proportioning feeding just pulverizing; Ultramicro grinding is to mix after 15 μ m together with other raw materials again, mixture homogeneity≤7%; Send into again soft granulator and granulate, pulverization rate≤6%;
Utilize syrup spray drip apparatus composite syrup evenly to be sprayed to the surface of particulate material, particle diameter 2mm.Finally be packaged as finished product by the weighed amount of automatic packaging.
Embodiment 3
(1) a soft particle creep feed that contains HFCS, comprises that the raw material of following parts by weight forms:
60 parts, rice, 4 parts of steam fish meal, 5 parts, flour, 4 parts of dehulled soybean meals, 2 parts, chyle fat powder, 10 parts of whey powders, 5 parts of milk powder, 2 parts of white granulated sugars, 1 part of trehalose, 1 part of HFCS, 20 parts of composite fermentation dregs of beans, 3 parts of glucose, 2 parts, soybean lecithin powder, 0.5 part of salt, 1.2 parts of calcium dihydrogen phosphates, 0.02 part of calcium formate, 0.5 part, amino acid, 0.1 part of complex enzyme, 0.4 part of B B-complex, 0.6 part of composite trace element;
Chyle fat powder is selected Canon or other commercially available prod.
Described whey powder is the preparation of roller drying technique, and wherein lactose is beta lactose;
Described complex enzyme consists of: protease 3 part, 5 parts of amylase.
Described amino acid masses ratio is Dlys:Dmet:Dthr:Dtrp:Dval=100:35:63:21:69.
Described B B-complex composition is 3 parts of vitamin As, 5 parts of vitamin Ds, 6 parts of vitamin Es.
Described composite trace element consists of: 3 parts, ferrous sulfate, 4 parts of manganese sulfates, 2 parts, zinc oxide, 6 parts of basic copper chlorides, 3 parts, copper sulphate, 3 parts of sodium selenites, 3 parts of cobalt chlorides, 3 parts of calcium iodates.
The preparation method of described composite fermentation dregs of beans is as follows:
Dregs of beans is pulverized to obtain to dregs of beans powder, by dregs of beans powder and corn protein powder in mass ratio 15:4 mix, obtain mix powder, in mix powder, add 8 times of water gagings and control temperature to 70 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, makes the pH value of mixture be controlled at 6.5 in enzymolysis process, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate, 0.5% sodium sulphate, then in 110 DEG C of moist heat sterilization 20min; To adding and mix bacillus subtilis seed liquor in fermentation medium, inoculum concentration is 8%, 30 DEG C of temperature, under the condition that pH value is 7, cultivate the pickled vegetable fermentation liquor that adds hydrolysed mix quality 35% after 34h after holding temperature keep 15 hours at 18 DEG C.Then the dry composite fermentation bean cake powder that obtains.
Described acid protease addition is 1% of dregs of beans powder quality, and AMS is 0.05% of bean cake powder quality, and described trypsase is 1% of dregs of beans powder quality;
Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:3; CICC10063 and CICC20030 seed liquor are mixed according to volume ratio after cultivating respectively.
Described pickle fermentation liquid and preparation method thereof is with example 1.
(2) preparation method: the resolvability components such as chyle fat powder, whey powder, milk powder, white granulated sugar, trehalose, soybean lecithin powder and calcium formate, by after proportioning feeding, are dissolved in HFCS, make composite syrup;
By rice, dehulled soybean meal and composite fermentation dregs of beans carry out after by proportioning feeding just pulverizing; Ultramicro grinding is to mix after 15 μ m together with other raw materials again, mixture homogeneity≤7%; Send into again soft granulator and granulate, pulverization rate≤6%;
Utilize syrup spray drip apparatus composite syrup evenly to be sprayed to the surface of particulate material, particle diameter 3mm.Finally be packaged as finished product by the weighed amount of automatic packaging.
Feeding effect checking: the soft particle creep feed that contains HFCS that adopts embodiment 1 to prepare does following checking
1 test material
1.1 test period
Tested in May, 2012, last 14 days.
1.2 test site
Kind pig farm, Fengshan, Liaoning Province.
1.3 experimental animal
Select totally 160 of the long white three way cross certified milk piglets of Du Luoke * great Bai *.
1.4 test daily rations
Control group and test group totally 2 processing, 8 repetitions of each processing are established in test.Experimental group is the soft particle creep feed that contains HFCS of the inventive example 1.Control group feed is the feed that contains conventional market sale fermented bean dregs powder;
2 test methods
2.1 experimental design
This test adopts single factor Random Design, select Du Luoke × length that 28 ages in days left and right wean pigs, average initial body weight be about 8.52 ± 0.72kg in vain × totally 160 of great Bai three way cross healthy growth pigs, by the close principle similar with hereditary basis of body weight, be divided at random 2 processing, the two kinds of different daily rations of feeding respectively.8 repetitions of each processing, 10 pigs of each repetition (circle), male and female half and half, experimental period is the rear the last fortnight of wean.14 days experimental periods, record feed intake, weigh test initial and last heavy, and calculate daily gain, daily ingestion amount and material anharmonic ratio.
2.2 feeding and management
Test pig is raised in closed pig house, raises well-ventilated on bed.Duration of test free choice feeding and drinking-water, without raising the phase in advance.By pig farm conventional program carry out disinfection, expelling parasite and immunity.
2.3 testing index
2.3.1 ration ingredient
The method that diet dry substance, thick protein, calcium and total phosphorus are recommended with reference to National Standard of the People's Republic of China GB/T6435-1986, GB/T6432-1994, GB/T6436-2002 and GB/T6437-2002 is respectively measured.
Daily ration sample respectively at 110 DEG C at 6mol/L hydrochloric acid hydrolysis 24h and 0 DEG C after performic oxidation 16h through hydrochloric acid hydrolysis for 24h automatic amino acid analyzer (L-8800 of Hitachi, Japan) measure 15 seed amino acids and sulfur-containing amino acid; At 110 DEG C, be hydrolyzed after 22h with 4mol/L NaOH, use high performance liquid chromatograph (Shimadzu LC-10A, Japan) to measure tryptophan.
2.3.2 growth performance
On an empty stomach individuality is weighed morning respectively at each on-test and while finishing, to repeat (circle) as unit record feed consumption rate, calculates average daily gain, average daily ingestion amount and feed and increases weight and compare.
2.4 statistical analysis
Test data is carried out after preliminary treatment with Excel software, adopts general linear model (general linearmodel, GLM) the modeling statistics analysis (SAS, 2001) of SAS8.2.
3 results and analysis
Two kinds of creep feeds contrast table to newborn piglet growth performance
| Control group one | Test group | |
| Starting weight (kg) | 8.55±0.77 | 8.49±0.70 |
| End heavy (kg) | 12.02±0.54 | 13.05±0.35 |
| Average daily gain (g/d) | 247.86±55A | 325.71±0.62B |
| Average daily ingestion amount (g/d) | 405.36 | 485.25 |
| Feed/weightening finish | 1.64 | 1.49 |
| Hair color marking (ten point system) | 5.5 | 8.5 |
Result shows, the growth performance of control group and test group comprises feed intake, average daily gain, material anharmonic ratio, all has clear improvement compared with control group: average daily gain improves 31.41%, and average daily ingestion amount improves 19.71%, and feedstuff-meat ratio reduces by 9.15%.Experimental group antibiotic dosage has reduced 65-80% than control group; Result of the test shows, feed of the present invention has obviously improved palatability and the feed intake of newborn piglet, has improved immunity and the general level of the health of newborn piglet.Thereby improve the income of raising pigs.
Claims (7)
1. a soft particle creep feed that contains HFCS, is made up of the raw material of following parts by weight:
Rice 50-60 part, steam fish meal 2-4 part, flour 3-5 part, dehulled soybean meal 2-4 part, chyle fat powder 1-2 part, whey powder 5-10 part, milk powder 3-5 part, white granulated sugar 1-2 part, trehalose 0.5-1, HFCS 0.5-1 part, composite fermentation dregs of beans 10-20 part, glucose 2-3 part, soybean lecithin powder 1-2 part, salt 0.3-0.5 part, calcium dihydrogen phosphate 0.8-1.2 part, calcium formate 0.01-0.02 part, amino acid 0.2-0.5 part, complex enzyme 0.05-0.1 part, B B-complex 0.3-0.4 part, composite trace element 0.2-0.6 part; Described complex enzyme consists of: protease 1-3 part, amylase 2-5 part;
It is characterized in that, the preparation method of described composite fermentation dregs of beans is as follows:
By bean cake powder and corn flour in mass ratio 15:2-4 mix, obtain mix powder, in mix powder, add 5-8 times of water gaging and control temperature to 50-70 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, in enzymolysis process, make the pH value of mixture be controlled at 5.5-6.5, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate, 0.5% sodium sulphate, then in 110-121 DEG C of moist heat sterilization 15-20min; In fermentation medium, add mixing bacillus subtilis seed liquor, inoculum concentration is 4-8%, temperature 25-30 DEG C, under the condition that pH value is 5-7, cultivate the pickled vegetable fermentation liquor that adds hydrolysed mix quality 20-35% after 30-34h after holding temperature keep 8-15 hour at 10-18 DEG C; Then the dry composite fermentation bean cake powder that obtains.
2. contain according to claim 1 the soft particle creep feed of HFCS, it is characterized in that, in the preparation method of described composite fermentation dregs of beans, acid protease addition is the 0.2-1% of bean cake powder quality, AMS addition is the 0.01-0.05% of bean cake powder quality, and described trypsase addition is the 0.4-1% of bean cake powder quality.
3. the soft particle creep feed that contains according to claim 1 HFCS, is characterized in that, in the preparation method of described composite fermentation dregs of beans, Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:1-3.
4. a method of preparing the soft particle creep feed that contains HFCS as described in claim as arbitrary in claim 1-3, comprises the steps:
By the resolvability component that contains chyle fat powder, whey powder, milk powder, white granulated sugar, trehalose, soybean lecithin powder and calcium formate by proportioning feeding after, be dissolved in HFCS, make composite syrup;
By rice, dehulled soybean meal and composite fermentation dregs of beans carry out after by proportioning feeding just pulverizing; Ultramicro grinding is to mix after 15 μ m together with other raw materials again, mixture homogeneity≤7%; Send into again soft granulator and granulate, pulverization rate≤6%;
Utilize syrup spray drip apparatus composite syrup evenly to be sprayed to the surface of particulate material, particle diameter 1-3mm; Be finally finished product by automatic packing balance quantitative package.
5. contain according to claim 4 the preparation method of the soft particle creep feed of HFCS, it is characterized in that, the preparation method of described fermented bean dregs is as follows: by bean cake powder and corn flour in mass ratio 15:2-4 mix, obtain mix powder, in mix powder, add 5-8 times of water gaging and control temperature to 50-70 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, in enzymolysis process, make the pH value of mixture be controlled at 5.5-6.5, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate, 0.5% sodium sulphate, then in 110-121 DEG C of moist heat sterilization 15-20min, in fermentation medium, add mixing bacillus subtilis seed liquor, inoculum concentration is 4-8%, temperature 25-30 DEG C, under the condition that pH value is 5-7, cultivate the pickled vegetable fermentation liquor that adds hydrolysed mix quality 20-35% after 30-34h after holding temperature keep 8-15 hour at 10-18 DEG C, then the dry composite fermentation bean cake powder that obtains, described acid protease addition is the 0.2-1% of bean cake powder quality, and AMS addition is the 0.01-0.05% of bean cake powder quality, and described trypsase addition is the 0.4-1% of bean cake powder quality.
6. according to the preparation method of soft particle creep feed who contains HFCS described in claim 4 or 5, it is characterized in that, described Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:1-3.
7. contain a preparation method for the soft particle creep feed of HFCS, soft particle creep feed raw material composition: 50 parts, rice, 2 parts of steam fish meal, 3 parts, flour, 4 parts of dehulled soybean meals, 2 parts, chyle fat powder, 5 parts of whey powders, 5 parts of milk powder, 2 parts of white granulated sugars, 1 part of trehalose, 1 part of HFCS, 10 parts of composite fermentation dregs of beans, 3 parts of glucose, 2 parts, soybean lecithin powder, 0.4 part of salt, 0.8 part of calcium dihydrogen phosphate, 0.01 part of calcium formate, 0.5 part, amino acid, 0.05 part of complex enzyme, 0.4 part of B B-complex, 0.6 part of composite trace element;
The preparation method of described composite fermentation dregs of beans is as follows:
Dregs of beans is pulverized to obtain to bean cake powder, by bean cake powder and corn protein powder in mass ratio 15:3 mix, obtain mix powder, in mix powder, add 6 times of water gagings and control temperature to 60 DEG C, add acid protease and AMS and trypsase, enzymolysis 4h, makes the pH value of fermentation medium be controlled at 5.5 in enzymolysis process, in the backward hydrolysed mix of enzymolysis, add 1.5% glucose, 0.1% magnesium sulfate and 0.5% sodium sulphate, then in 121 DEG C of moist heat sterilization 20min; In fermentation medium, add mixing bacillus subtilis seed liquor, inoculum concentration is 5%, under the condition that 25 DEG C of temperature, pH value are 6, cultivates after 32h, adds the pickled vegetable fermentation liquor of hydrolysed mix quality 25%, and holding temperature keeps 8 hours at 15 DEG C; Then the dry composite fermentation bean cake powder that obtains of spraying;
Bacillus subtilis strain is respectively CICC10063 and CICC20030; The volume ratio of described CICC10063 and CICC20030 seed liquor is 5:1; CICC10063 and CICC20030 seed liquor are mixed according to volume ratio after cultivating respectively;
Described acid protease addition is 0.6% of bean cake powder quality, and AMS addition is 0.03% of bean cake powder quality, and described trypsase addition is 0.7% of bean cake powder quality.
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| CN103734549B (en) * | 2014-01-27 | 2015-07-08 | 辽宁禾丰牧业股份有限公司 | Large-grain milk replacer and preparing method thereof |
| CN103750068A (en) * | 2014-01-27 | 2014-04-30 | 辽宁禾丰牧业股份有限公司 | Mixed milk replacer and preparation method thereof |
| CN104068234A (en) * | 2014-05-10 | 2014-10-01 | 滁州红琊山养殖有限公司 | Stomach invigorating and health protecting pig feed and preparation method thereof |
| CN104186934A (en) * | 2014-07-24 | 2014-12-10 | 山东和康源集团有限公司 | Creep feed specially used for piglet and preparation method thereof |
| CN104381696A (en) * | 2014-12-01 | 2015-03-04 | 云南西南红饲料有限公司 | Method for preparing piglet creep feed |
| CN105309807A (en) * | 2015-11-05 | 2016-02-10 | 中粮饲料有限公司 | Nutrient composition for animals and preparation method thereof |
| CN107259160B (en) * | 2017-08-04 | 2020-08-21 | 四川东坡中国泡菜产业技术研究院 | Method for preparing pig feed by using pickled vegetable salinized water |
| CN114158650B (en) * | 2021-11-30 | 2024-02-23 | 河南诺尔饲料有限公司 | Fermented full-mixed soft granule feed for young ruminants and preparation method thereof |
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