CN105639090A - Preparation method of livestock and poultry acidification and glycolysis feed - Google Patents
Preparation method of livestock and poultry acidification and glycolysis feed Download PDFInfo
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Abstract
The invention relates to a preparation method of a livestock and poultry acidification and glycolysis feed. The method comprises the following steps: crushing a starch-containing product, mashing the crushed starch-containing product, carrying out enzymatic hydrolysis to prepare a residue-containing saccharification liquid or carrying out solid-liquid separation to obtain a residue-free saccharification liquid; inoculating the residue-containing saccharification liquid to citric acid producing strains, and carrying out solid-liquid separation to obtain acid residues and a citric acid fermentation liquid; inoculating the residue-free saccharification liquid to lactic acid production strains, fermenting the inoculated saccharification liquid, and acidifying the fermented saccharification liquid to prepare a lactic acid fermentation liquid; concentrating one or more of the lactic acid fermentation liquid and the citric acid fermentation liquid to prepare an organic acid concentrate liquid; mixing one or more of sugar residues and acid residues with a feed raw material, a composite strain liquid and a compound enzyme, and carrying out enzymatic hydrolysis fermentation to prepare glycolysis residue meal; and mixing the organic acid concentrate liquid and the glycolysis residue meal with one or more of feeding inorganic acids, organic acid salts, carbohydrates and grease to prepare the livestock and poultry acidification and glycolysis feed. The livestock and poultry acidification and glycolysis feed prepared in the invention is rich in organic acids, small peptides, oligosaccharides, probiotics and other nutrients, and has the characteristics of diarrhea resistance, good palatability and easy digestion.
Description
Technical field
The invention belongs to feed, zymin and microbial technology field, it is specifically related to the preparation method of a kind of livestock and poultry acidifying and glycolysis feed.
Background technology
In recent years, food safety and environmental pollution be governments at all levels of various countries extensive concern extremely, concerning common people's health and lives safety. Livestock product safety requires feed no drug residue, environmental protection requirement feed low stain. Past we to talk feed be " effectively, safety ", now the Ministry of Agriculture of country emphasize again and again ' safety, effectively, low stain ". It is well known that microbiotic is since coming out, it takes precautions against disease for livestock and poultry and stimulating growth have received good effect really. But As time goes on, the defect of microbiotic is completely exposed: one is that microbiotic easily residues in the animal products such as meat, egg and milk, causing human body Liver and kidney function burden or canceration occurs, harm humans is healthy; Two is that livestock product local flavor is declined; Three is cause mankind itself's resistance to increase, and pollutes the physical environment such as soil and water source. Therefore, some countries have prohibited use. 1974, the European Community forbids using penicillin, tsiklomitsin in feed, forbid in December, 1998 in feed, use the happy mycin of Spiramycin Base, Zinc-bacitracin, virginiamycin and peptide, forbid in October, 1999 using chlorine fluorine benzyladenine (arprinocide) and isopropyl nitre to reach azoles (dinitolmide) in feed. , it may also be useful to citric acid, lactic acid, fumaric acid, probiotic bacterium, oligose and active small peptide substitute or reduce use microbiotic, therefore producing safe animal products enjoys national governments to pay close attention to.
China is population big country, Ye Shi aquaculture big country. 2014, the livestock industry output value reached 28,260 hundred million yuan, and meat, birds, beasts and eggs, milk and output of aquatic products reach 85,305 ten thousand tons, 2,800 ten thousand tons, 3,725 ten thousand tons and 5,300 ten thousand tons respectively. With the development of aquaculture, feed industrial development speed is surprising. According to Ministry of Agriculture's statistic data, within 2014, national business feed output reaches 1.97 hundred million tons. Wherein, complete feed 1.69 hundred million tons, concentrated fodder 2,151 ten thousand tons, Preblend 6,400,000 tons, comprises pig feed output 8,686 ten thousand tons, fowl material output 9,000 ten thousand tons, aquatic feeds 5,300 ten thousand tons, 8,500,000 tons, cattle and sheep feed, 3,500,000 tons, the feeds such as rabbit racoon dog pet.
It is well known that the difficult point of the exploitation of feeds product, development is the feed for piglet. How to improve children's animal and fowl fodder palatability in age, digestibility and reduction or reduce antinutritional factor and become the problem that animal nutrition man is actively inquired in recent years. With regard to suckling piglet, its metabolism is vigorous, allometry is rapid, to the requirement height of diet nutrient material, but the function of its digestive tube is unsound, the weight of digestive tube and volume is less and send out the region between the heart and the diaphragm incomplete, gastric acid secretion is not enough, gastrointestinal micro-flora easily becomes, and it is unsound that Digest enzyme sends out the region between the heart and the diaphragm, and digestive ferment secretion is not enough. Suckling piglet protein digestion is bad, and protein easily produces some spoilage product at hindgut, cadaverine, putrescine, histamine etc., stimulates intestinal mucosa, and infringement intestinal villi film, causes intestinal contents osmotic pressure to raise, and enteron aisle dehydration causes diarrhoea. Vegetable-protein is irritated, the piglet before 8 weeks age, to vegetable-protein easily allergy cause intestinal mucosa impaired, affect the absorption to nutrient, bacterium is easily invaded and causes suffering from diarrhoea. Therefore, by biotechnologys such as fermentation, enzymolysis, exploitation nutrition height, easy to digest, good palatability, be rich in citric acid, lactic acid, fumaric acid and probiotic bacterium, oligose, active small peptide the feed for piglet be an extremely urgent and significant major issue.
Feeder acidulant reduces the pH value of feed in digestive tube as a kind of, for animal provides the new additive agent of the suitableeest digestive tract environment, is at home and abroad used widely. Souring agent is widely used in the feed of the animals such as poultry, piglet, beef cattle, milk cow, sheep. Feeder acidulant can improve daily ration palatability, increases piglet food consumption, and early-weaned piglets uses fumaric acid to make rate of body weight gain improve 5.1%, and feedstuff-meat ratio reduces by 6.5%. Conventional souring agent has the mineral acids such as organic acid and formic acid, propionic acid, hydrochloric acid, phosphoric acid such as citric acid, fumaric acid, lactic acid, dystopy acid. Different souring agents respectively has its feature, but uses the most extensive, effect preferably citric acid, fumaric acid and compound acid. Fumaric acid has broad-spectrum sterilization and bacteriostatic activity, as added the fumaric acid of 0.2%��0.4% concentration in feed, can kill staphylococcus and suis; 0.4% can kill intestinal bacteria; Toxigenic fungi is had by more than 2% concentration to be killed and restraining effect.
Probiotic bacterium (Probiotics) has another name called probiotics, probiotics, regulator, profit raw element, bacteria-promoting agent etc. Nineteen ninety Sogaard proposes probiotics concept comparatively accurately: namely " Probiotics be take in participate in microbial balance in intestines in animal body have directly by strengthening the restraining effect to intestinal toxic microflora; or carry out preventing disease by strengthening non-specific immune function; and indirectly play the living microorganism culture promoting growth of animal effect and improving feed conversion rate ", be called as human body and the big nutrient substance of animal the 8th. Test proves: probiotic bacterium can replace or reduce microbiotic, and by consuming oxygen, secretion antibacterial peptide and lactic acid, suppress spoilage organism growth, control grice diarrhoea; After using probiotic bacterium, livestock and poultry anti pathologic immunity power significantly improves.
Oligose (or claiming oligosaccharides) is the novel starch sugar generated by the katalysis of enzyme by starch, and it integrates nutrition, health care, dietotherapy, is widely used in feed, food, healthcare products, beverage, medicine and other fields. Test proves: oligose can replace or reduce microbiotic, effectively promotes growth of probiotics breeding, suppresses spoilage organism growth, control grice diarrhoea; After using oligose, livestock and poultry anti pathologic immunity power significantly improves; Oligose can promote that enteron aisle is to the absorption of calcium, iron, zine ion effectively.
Little peptide is the digestion product of protein, it can intactly be absorbed enters animal circulating system, can improve animal to the utilization ratio of protein, obviously strengthen animal immunizing power and disease resistance ability, effectively improve the quality of animal product, thus play the production potential of animal better.
With regard to fodder acidulant, current domestic feeder acidulant is fermentation liquor decolouring mostly, from the compound product of food grade organic acid handed over and after crystallization, its organic acid is single, and tart flavour is pained. Food grade organic acid produces all will through decolouring, from friendship or crystallization production process. The material such as VITAMIN and pigment or nutrition are sloughed in decolouring, are slough mineral element from friendship, such as calcium, phosphorus, magnesium, sodium, potassium, chlorine, sulphur, iron, copper, zinc, manganese, iodine, cobalt, selenium etc. Decolour and from contacts toward a large amount of waste water, waste liquid all can be produced, pollute heavy, cost height, and tart flavour bitterness can be caused. Therefore, feeding nondiscoloration, not from handing over product will be the direction of following development. But, not yet find nondiscoloration at present, not from the feeds product that the organic acid fermentation liquid handed over is directly composite. Producing due to this patent organic acid and take solid state fermentation slag dregs of rice feed adsorption technology, fermentation is rear without decolouring, from friendship or crystallization production process. Therefore, the production technique of this patent and food grade organic acid is essentially different.
Before this, applicant Liu Xuan instrument and Meng Long once declared patent " preparation method of a kind of tartaric acid fodder additives " (number of patent application: CN201510424595.8) the elaboration preparation method of livestock and poultry new feed souring agents. But the preparation method of this patent exists following defect: one, not containing functional activity nutritive ingredients such as profitable probliotics, oligose, little peptides, Antidiarrheic effect is poor, substitute or reduce feeding antibiotic weak effect; Two, almost not containing conventional nutrients such as protein, robust fibre, crude fat, VITAMIN and mineral elements, the conventional nutrients such as a small amount of protein, robust fibre, crude fat, VITAMIN and mineral element are all come from fruit, instead of come from sugar slag and acid sludge enzymatic hydrolysis and fermentation; Three, with the addition of a large amount of non-nutritive white carbon blacks as carrier, seriously have impact on the palatability of livestock and poultry and food consumption; Four, being only a kind of additive, addition is less than normal, in small-sized plant in-convenience in use; Five, production organic acid fermentation process produces sugared slag and acid sludge are not effectively addressed, seriously polluted.
Summary of the invention
The present invention is intended to overcome above-mentioned, functional activity nutritive ingredient shortage pained from the fodder acidulant handed over through decolouring and contain the deficiencies such as a large amount of non-nutritive carrier white carbon blacks, there is provided one to be rich in the nutritive ingredients such as lactic acid, citric acid, little peptide, oligose, probiotic bacterium, other organic acid, protein, robust fibre, crude fat, VITAMIN and mineral element, there is livestock and poultry acidifying and the glycolysis feed of the features such as diarrhea, good palatability, amino acid balance, nutrition height, easy to digest and antinutritional factor content are low.
The technical solution used in the present invention is as follows:
A preparation method for livestock and poultry acidifying and glycolysis feed, comprises the following steps:
1., the preparation of the wet base of sugar slag, citric acid fermentation broth and the wet base of citric acid acid sludge:
To pulverize containing starch product, and add water and size mixing, and add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access produces citric acid strain fermentation some hours, through the solid-liquid separation wet base of obtained citric acid acid sludge and citric acid fermentation broth;
Maybe will pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; Citric acid strain fermentation is produced after some hours, directly obtained citric acid fermentation broth without access in slag saccharified liquid;
2., the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid;Without slag saccharified liquid accesses lactic acid producing bacterial classification, ferment some hours when Continuous Flow hydro-oxidation calcium solution, then add after dilute sulphuric acid carries out acidifying, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
Maybe will pulverize containing starch product, and add water and size mixing, add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access lactic acid producing bacterial classification, ferment some hours when flowing hydro-oxidation calcium solution, add after dilute sulphuric acid carries out acidifying again, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
3., the preparation of the wet base of sugar slag, other organic acid fermentation liquid and the wet base of other organic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; In without slag saccharified liquid, access one or more other organic acid strain fermentations some hours, obtain the wet base of other organic acid acid sludge and other organic acid fermentation liquid through solid-liquid separation;
Maybe will pulverize containing starch product, and add water and size mixing, add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly one or more other organic acid strain fermentations of access access some hours, obtain the wet base of other organic acid acid sludge and other organic acid fermentation liquid through solid-liquid separation;
Wherein, other organic acid refers to the organic acid except lactic acid, citric acid;
4., the preparation of fermentation source organic acid concentrated solution:
After one in lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid directly being concentrated, prepare fermentation source organic acid concentrated solution;
Or by concentrated after more than one mixing in lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid, or mixing after concentrating respectively, prepare fermentation source organic acid concentrated solution;
5., the preparation of the wet base of the glycolysis slag dregs of rice:
Enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge, the wet base of other organic acid acid sludge are mixed with composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
Or enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge, the wet base of other organic acid acid sludge are mixed with feedstuff raw material, clean water, composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
6., the preparation of livestock and poultry acidifying and glycolysis feed:
After wet to fermentation source organic acid concentrated solution and glycolysis slag dregs of rice base is mixed, direct production livestock and poultry acidifying and the wet base of glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively;
Or after fermentation source organic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed with one or more in commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat, the wet base of direct production livestock and poultry acidifying and glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively.
Preferably, described a kind of livestock and poultry acidifying and the preparation method of glycolysis feed, comprise the following steps
1., the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
To pulverize containing starch product, and add water and size mixing, and add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access produces citric acid strain fermentation some hours, through the solid-liquid separation wet base of obtained citric acid acid sludge and citric acid fermentation broth;
2., the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid;Without slag saccharified liquid accesses lactic acid producing bacterial classification, ferment some hours when Continuous Flow hydro-oxidation calcium solution, then add after dilute sulphuric acid carries out acidifying, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
3., the preparation of fermentation source organic acid concentrated solution:
After one in lactic acid fermentation liquid, citric acid fermentation broth directly being concentrated, prepare fermentation source organic acid concentrated solution;
Or by concentrated after more than one mixing in lactic acid fermentation liquid, citric acid fermentation broth, or mixing after concentrating respectively, prepare fermentation source organic acid concentrated solution;
4., the preparation of the wet base of the glycolysis slag dregs of rice:
Enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge are mixed with composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
Or enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge are mixed with feedstuff raw material, clean water, composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
5., the preparation of livestock and poultry acidifying and glycolysis feed:
After citric acid and lactic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed, direct production livestock and poultry acidifying and the wet base of glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively;
Or after citric acid and lactic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed with one or more in commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat, the wet base of direct production livestock and poultry acidifying and glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively.
Further, other organic acid fermentation liquid described is a kind of fermented liquid of organic acid or multiple fermented liquid in the Ministry of Agriculture of country fodder additives catalogue, a kind of fermented liquid being mainly in fumaric acid, oxysuccinic acid, a-ketoglutaric acid, succsinic acid, tartrate, acetic acid, propionic acid, butyric acid, xitix, Whitfield's ointment, gibberic acid, kojic acid, gluconic acid, propylenedicarboxylic acid or multiple fermented liquid;
Further, described feedstuff raw material is one or more of feedstuff raw material in country's Ministry of Agriculture feedstuff raw material catalogue, it is mainly dregs of beans, cotton dregs, rapeseed meal, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, Zein powder, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, crack rice, wheat, wheat bran, rice bran, flour, oat, barley, Ipomoea batatas, soybean protein isolate, soybean protein concentrate, pasture powder, herbal medicine, fruit and vegetable, sugar molasse syrup and food-processing industry byproduct, or above-mentioned feedstuff raw material dilated product in one or more,
Further, described commercially available feeding non-fermented source organic acid be in the country's Ministry of Agriculture fodder additives catalogue non-fermented organic acid one or more, be mainly in formic acid, phenylformic acid, tartrate, fumaric acid, Sorbic Acid one or more;
Further, described commercially available feeding mineral acid is one or more of mineral acid in country's Ministry of Agriculture fodder additives catalogue, be mainly in phosphoric acid, hydrochloric acid, sulfuric acid one or more;
Further, described organic acid salt is one or more of organic acid salt in country's Ministry of Agriculture fodder additives catalogue, be mainly in calcium formiate, Sodium Propionate, calcium propionate, Sodium propanecarboxylate, Sodium Benzoate, sodium sorbate, potassium sorbate, Tripotassium Citrate, Trisodium Citrate, citrate of lime one or more;
Further, described sugar molasse syrup is one or more of sugar molasse syrup in country's Ministry of Agriculture feedstuff raw material catalogue, be mainly in sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, high fructose syrup/powder, malt syrup/powder, Sugar cane molasses, beet sirup one or more;
Further, described liquid fat is one or more of liquid fat in country's Ministry of Agriculture feedstuff raw material catalogue, be mainly in soybean oil and oil in water emulsion, Semen Maydis oil and oil in water emulsion, plam oil and oil in water emulsion, Rice pollard oil and oil in water emulsion, Oleum Cocois and oil in water emulsion, fish oil and animal oil and oil in water emulsion, peanut oil and oil in water emulsion, rapeseed oil and oil in water emulsion, tea oil and oil in water emulsion thereof one or more.
Preferably, other organic acid fermentation liquid described be fumaric acid, succsinic acid, tartrate, acetic acid, propionic acid, butyric acid, in a kind of fermented liquid or multiple fermented liquid;
Preferably, described feedstuff raw material is dregs of beans, cotton dregs, Zein powder, rice protein powder, DDGS, wheat bran, rice bran, pasture powder, herbal medicine, fruit and vegetable, sugar molasse syrup and food-processing industry byproduct, or above-mentioned feedstuff raw material dilated product in one or more;
Preferably, described commercially available feeding non-fermented source organic acid is one or more in formic acid, phenylformic acid, fumaric acid;
Preferably, described commercially available feeding mineral acid is phosphoric acid;
Preferably, described organic acid salt is one or more in calcium formiate, Sodium propanecarboxylate, Trisodium Citrate, citrate of lime;
Preferably, described sugar molasse syrup is one or more in sucrose, glucose, glucose syrup, Sugar cane molasses, beet sirup;
Preferably, described liquid fat is one or more in soybean oil and oil in water emulsion, Semen Maydis oil and oil in water emulsion, plam oil and oil in water emulsion, Rice pollard oil and oil in water emulsion, Oleum Cocois and oil in water emulsion, fish oil and animal oil and oil in water emulsion thereof.
Further, step 4. in the preparation condition of fermentation source organic acid concentrated solution as follows:
(1) when, preparing lactic acid fermentation liquid: lactic acid producing bacterial classification leavening temperature: 20��60 DEG C; Fermentation pH:5��9; Fermented type: anaerobically fermenting; Fermentation time: 6-168 hour;
(2) when, preparing citric acid fermentation broth: produce citric acid waste residue strain fermentation temperature: 10��40 DEG C; Fermentation pH: nature; Fermented type: aerobic fermentation; Fermentation time: 6-216 hour;
(3), the weight ratio of lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid is 100:0��1000:0��500.
Preferably, step 4. in the preparation condition of fermentation source organic acid concentrated solution as follows:
(1) when, preparing lactic acid fermentation liquid: lactic acid producing bacterial classification leavening temperature: 40��50 DEG C; Fermentation pH:6��8; Fermented type: anaerobically fermenting; Fermentation time: 48-60 hour;
(2) when, preparing citric acid fermentation broth: produce citric acid waste residue strain fermentation temperature: 28��32 DEG C; Fermentation pH: nature; Fermented type: aerobic fermentation; Fermentation time: 60-72 hour;
(3), the weight ratio of lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid is 100:50��80:20��40.
Further, step 5. in the preparation condition of the wet base of the glycolysis slag dregs of rice as follows:
(1), the weight ratio of the wet base of sugar slag, the wet base of citric acid acid sludge, the wet base of other organic acid acid sludge and feedstuff raw material, clean water, composite bacteria liquid, prozyme is 100:0��500:0��300:0��3000:0��2000:0.5��50:0.0001��10;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast strain liquid: the number of viable of Bacillus licheniformis kind liquid is than being 100:0��200:0��100;
(3), the commercial protein enzyme in prozyme: zytase: dextranase: mannase: the weight ratio of polygalacturonase is 100:0��1000:0��1000:0��1000:0��1000;
(4), enzymatic hydrolysis and fermentation temperature: 10��60 DEG C, first there is anaerase hydrolysis and fermentation 12-240 hour after oxygenase hydrolysis and fermentation initial pH:4��9 of enzymatic hydrolysis and fermentation.
Preferably, step 5. in the preparation condition of the wet base of the glycolysis slag dregs of rice as follows:
(1), the weight ratio of the wet base of sugar slag, the wet base of citric acid acid sludge, the wet base of organic acid acid sludge and feedstuff raw material, clean water, composite bacteria liquid, prozyme is 100:0��80:0��40:200��800:0��200:10��20:0.005��2;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast strain liquid: the number of viable of Bacillus licheniformis kind liquid is than being 100:10��30:5��10;
(3), the commercial protein enzyme in prozyme: zytase: dextranase: mannase: the weight ratio of polygalacturonase is 100:60��80:60��80:60��80:30��50;
(4), enzymatic hydrolysis and fermentation temperature: 30��40 DEG C, first there is anaerase hydrolysis and fermentation 72-96 hour after oxygenase hydrolysis and fermentation initial pH:6��8 of enzymatic hydrolysis and fermentation.
Further, step 6. in the preparation condition of livestock and poultry acidifying and glycolysis feed as follows:
The weight ratio of the wet base of fermentation source organic acid concentrated solution, the glycolysis slag dregs of rice and commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat is 100:0��2000:0��300:0��300:0��300:0��500:0��500.
Preferably, step 6. in the preparation condition of livestock and poultry acidifying and glycolysis feed as follows:
The weight ratio of the wet base of fermentation source organic acid concentrated solution, the glycolysis slag dregs of rice and commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat is 100:200��800:20��40:10��20:10��20:50��100:10��20.
The useful effect of the present invention is: livestock and poultry acidifying prepared by the present invention and glycolysis feed, it is rich in the nutritive ingredients such as lactic acid, citric acid, little peptide, oligose, probiotic bacterium, other organic acid, protein, robust fibre, crude fat, VITAMIN and mineral element, there is the features such as diarrhea, good palatability, amino acid balance, nutrition height, easy to digest and antinutritional factor content is low.
Embodiment
Embodiment 1
A preparation method for livestock and poultry acidifying and glycolysis feed, comprises the following steps:
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
By 100kg crush maize, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid. In 300kg band slag saccharified liquid, the aspergillus niger strain liquid 25kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 325kg. Through the solid-liquid separation wet base of obtained 90kg citric acid acid sludge and 235kg citric acid fermentation broth.
(2), the preparation of citric acid concentrated solution:
After directly being concentrated by 235kg citric acid fermentation broth, prepare 120kg citric acid concentrated solution.
(3), the preparation of the wet base of the glycolysis slag dregs of rice:
In the wet base of 90kg citric acid acid sludge, after adding 60kg dregs of beans, 15kg molasses, 50kg clean water, 12kg composite bacteria liquid, the mixing of 0.08kg prozyme, at temperature 30��40 DEG C and initial pH:6��8 condition, after first having oxygen, anaerobically fermenting is after 96 hours, the wet base of the obtained 218kg glycolysis slag dregs of rice.
(4) preparation of livestock and poultry acidifying and glycolysis feed:
After wet to 120kg citric acid concentrated solution and 218kg glycolysis slag dregs of rice base is mixed, the livestock and poultry acidifying of direct production 338kg water content 50-60% and the wet base of glycolysis feed, or dry and make 155kg livestock and poultry acidifying and the dry base of glycolysis feed.
Or after wet to 120kg citric acid concentrated solution and 218kg glycolysis slag dregs of rice base and 100kg fumaric acid solution, 30kg phosphoric acid are mixed, dry and make 285kg livestock and poultry acidifying and the dry base of glycolysis feed.
Embodiment 2
A preparation method for livestock and poultry acidifying and glycolysis feed, comprises the following steps:
(1) preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg is cracked rice pulverizing, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid. Through the solid-liquid separation obtained 20kg wet base of sugar slag and 280kg without slag saccharified liquid.
At 280kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 38-42 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 300kg. Adding 50kg20% dilution heat of sulfuric acid in 300kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 20kg lactic acid acid sludge and calcium sulfate wet base, 330kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(2), the preparation of lactic acid concentrated solution:
After directly being concentrated by 330kg lactic acid fermentation liquid again, prepare 100kg lactic acid concentrated solution.
(3), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 20kg wet base of sugar slag, after adding 60kg dregs of beans, 10kg molasses, 50kg clean water, 8kg composite bacteria liquid, the mixing of 0.05kg prozyme, temperature 30��40 DEG C, with, under the condition of initial pH:6��8, anaerobically fermenting is after 96 hours after first having oxygen, obtains the wet base of the 145kg glycolysis slag dregs of rice.
(4) preparation of livestock and poultry acidifying and glycolysis feed:
After wet to 100kg lactic acid concentrated solution and 145kg glycolysis slag dregs of rice base is mixed, the livestock and poultry acidifying of direct production 245kg water content 50-60% and the wet base of glycolysis feed, or dry and make 155kg livestock and poultry acidifying and the dry base of glycolysis feed.
Or after 100kg lactic acid concentrated solution, the wet base of the 145kg glycolysis slag dregs of rice and 100kg fumaric acid solution, 30kg phosphoric acid are mixed, dry and make 255kg livestock and poultry acidifying and the dry base of glycolysis feed.
Embodiment 3
(1), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
By 100kg crush maize, add after 250kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 350kg band slag saccharified liquid. Through the solid-liquid separation obtained 85kg wet base of sugar slag and 265kg without slag saccharified liquid.
At 265kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 285kg. Adding 40kg20% dilution heat of sulfuric acid in 285kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 16kg lactic acid acid sludge and calcium sulfate wet base, 309kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(2), the preparation of lactic acid concentrated solution:
After directly being concentrated by 309kg lactic acid fermentation liquid again, prepare 90kg lactic acid concentrated solution.
(3), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 85kg wet base of sugar slag, after adding 10kg dregs of beans, 10kg fish meal, 5kg molasses, 10kg composite bacteria liquid, the mixing of 0.02kg prozyme, temperature 30��40 DEG C, with, under the condition of initial pH:6��8, anaerobically fermenting is after 84 hours after first having oxygen, obtains the wet base of the 100kg glycolysis slag dregs of rice.
(4), the preparation of livestock and poultry acidifying and glycolysis feed:
After wet to 90kg lactic acid concentrated solution and 100kg glycolysis slag dregs of rice base is mixed, dry and make 110kg livestock and poultry acidifying and glycolysis feed.
Or after wet to 90kg lactic acid concentrated solution and 100kg glycolysis slag dregs of rice base and 50kg fumaric acid solution, 30kg phosphoric acid are mixed, dry and make 150kg livestock and poultry acidifying and glycolysis feed.
Embodiment 4
(1), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
50kg corn and 50kg are cracked rice after co-grinding, add after 220kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 320kg band slag saccharified liquid. Through the solid-liquid separation obtained 50kg wet base of sugar slag and 270kg without slag saccharified liquid.
At 270kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 290kg. Adding 40kg20% dilution heat of sulfuric acid in 290kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 18kg lactic acid acid sludge and calcium sulfate wet base, 312kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(2), the preparation of lactic acid concentrated solution:
After directly being concentrated by 312kg lactic acid fermentation liquid again, prepare 90kg lactic acid concentrated solution.
(3), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 50kg wet base of sugar slag, after adding 30kg Zein powder, 6kg molasses, 20kg clean water, 10kg composite bacteria liquid, the mixing of 0.03kg prozyme, temperature 30��40 DEG C, with, under the condition of initial pH:6��8, anaerobically fermenting is after 84 hours after first having oxygen, obtains the wet base of the 100kg glycolysis slag dregs of rice.
(4), the preparation of livestock and poultry acidifying and glycolysis feed:
After wet to 90kg lactic acid concentrated solution and 100kg glycolysis slag dregs of rice base is mixed, the livestock and poultry acidifying of direct production 190kg water content 50-60% and glycolysis feed, or dry and make 110kg livestock and poultry acidifying and glycolysis feed.
Or after wet to 90kg lactic acid concentrated solution and 100kg glycolysis slag dregs of rice base and 50kg succinic acid solution, 30kg phosphoric acid are mixed, dry and make 150kg livestock and poultry acidifying and glycolysis feed.
Embodiment 5
(1), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg is cracked rice pulverizing, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid. Through the solid-liquid separation obtained 20kg wet base of sugar slag and 280kg without slag saccharified liquid.
At 280kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 300kg. Adding 50kg20% dilution heat of sulfuric acid in 300kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 20kg lactic acid acid sludge and calcium sulfate wet base, 330kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(2), the preparation of lactic acid concentrated solution:
After directly being concentrated by 330kg lactic acid fermentation liquid again, prepare 100kg lactic acid concentrated solution.
(3), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 20kg wet base of sugar slag, after adding 30kg dregs of beans, 20k wheat bran, 10kg rice bran, 10kg alfalfa meal, 10kg molasses, 60kg clean water, 8kg composite bacteria liquid, the mixing of 0.05kg prozyme, temperature 30��40 DEG C is with under the condition of initial pH:6��8, after first having oxygen, anaerobically fermenting is after 72 hours, the wet base of the obtained 165kg glycolysis slag dregs of rice.
(4), the preparation of livestock and poultry acidifying and glycolysis feed:
After wet to 100kg lactic acid concentrated solution and 165kg glycolysis slag dregs of rice base is mixed, dry and make 155kg livestock and poultry acidifying and glycolysis feed.
Or after 100kg lactic acid concentrated solution, the wet base of the 165kg glycolysis slag dregs of rice, 100kg fumaric acid solution, 30kg phosphoric acid and 50kg molasses, 10kg emulsified fat are mixed, dry and make 290kg livestock and poultry acidifying and glycolysis feed.
Embodiment 6
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
By 60kg crush maize, add after 150kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 210kg band slag saccharified liquid.
In 210kg band slag saccharified liquid, the aspergillus niger strain liquid 15kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 220kg. Through the solid-liquid separation wet base of obtained 50kg citric acid acid sludge and 170kg citric acid fermentation broth.
(2), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg is cracked rice pulverizing, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid. Through the solid-liquid separation obtained 20kg wet base of sugar slag and 280kg without slag saccharified liquid.
At 280kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, at Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 300kg. Adding 50kg20% dilution heat of sulfuric acid in 300kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 20kg lactic acid acid sludge and calcium sulfate wet base, 330kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(3), the preparation of lactic acid and citric acid concentrated solution:
After 330kg lactic acid fermentation liquid and the mixing of 170kg citric acid fermentation broth being concentrated again, prepare 175kg lactic acid and citric acid concentrated solution.
(4), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 20kg wet base of sugar slag and the wet base of 50kg citric acid acid sludge, after adding 30kg dregs of beans, 10kg molasses, 20kg clean water, 12kg composite bacteria liquid, the mixing of 0.08kg prozyme, temperature 30��40 DEG C is with under the condition of initial pH:6��8, after first having oxygen, anaerobically fermenting is after 96 hours, the wet base of the obtained 128kg glycolysis slag dregs of rice.
(5), the preparation of livestock and poultry acidifying and glycolysis feed:
After 175kg lactic acid and citric acid concentrated solution, the wet base of the 128kg glycolysis slag dregs of rice are mixed, dry and make 175kg livestock and poultry acidifying and glycolysis feed.
Or after 175kg lactic acid and citric acid concentrated solution, the wet base of the 128kg glycolysis slag dregs of rice, 100kg fumaric acid solution, 30kg phosphoric acid are mixed, dry and make 275kg livestock and poultry acidifying and glycolysis feed.
Embodiment 7
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
By 100kg crush maize, add after 250kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 350kg band slag saccharified liquid.
In 350kg band slag saccharified liquid, the aspergillus niger strain liquid 25kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 350kg. Through the solid-liquid separation wet base of obtained 90kg citric acid acid sludge and 260kg citric acid fermentation broth.
(2), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
By 100kg crush maize, add after 250kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 350kg band slag saccharified liquid.Through the solid-liquid separation obtained 85kg wet base of sugar slag and 265kg without slag saccharified liquid.
At 265kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 285kg. Adding 40kg20% dilution heat of sulfuric acid in 285kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 16kg lactic acid acid sludge and calcium sulfate wet base, 309kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(3), the preparation of lactic acid and citric acid concentrated solution:
After 309kg lactic acid fermentation liquid and the mixing of 260kg citric acid fermentation broth being concentrated again, prepare 220kg lactic acid and citric acid concentrated solution.
(4), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 85kg wet base of sugar slag and the wet base of 90kg citric acid acid sludge, after adding 10kg molasses, 10kg composite bacteria liquid, the mixing of 0.03kg prozyme, temperature 30��40 DEG C, with, under the condition of initial pH:6��8, anaerobically fermenting is after 84 hours after first having oxygen, obtains the wet base of the 185kg glycolysis slag dregs of rice.
(5), the preparation of livestock and poultry acidifying and glycolysis feed:
After 220kg lactic acid and citric acid concentrated solution, the wet base of the 185kg glycolysis slag dregs of rice are mixed, the livestock and poultry acidifying of direct production 405kg water content 50-60% and glycolysis feed, or dry and make 190kg livestock and poultry acidifying and glycolysis feed.
Or after 220kg lactic acid and citric acid concentrated solution, the wet base of the 185kg glycolysis slag dregs of rice and 50kg fumaric acid solution, 30kg phosphoric acid are mixed, dry and make 250kg livestock and poultry acidifying and glycolysis feed.
Embodiment 8
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
By 60kg crush maize, add after 150kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 210kg band slag saccharified liquid.
In 210kg band slag saccharified liquid, the aspergillus niger strain liquid 15kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 220kg. Through the solid-liquid separation wet base of obtained 50kg citric acid acid sludge and 170kg citric acid fermentation broth.
(2), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg corn and 100kg are cracked rice after co-grinding, add after 440kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 640kg band slag saccharified liquid. Through the solid-liquid separation obtained 100kg wet base of sugar slag and 540kg without slag saccharified liquid.
At 540kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 50kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 580kg. Adding 80kg20% dilution heat of sulfuric acid in 580kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 36kg lactic acid acid sludge and calcium sulfate wet base, 625kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(3), the preparation of lactic acid and citric acid concentrated solution:
Mixing after 625kg lactic acid fermentation liquid and 170kg citric acid fermentation broth being concentrated respectively, prepares 230kg lactic acid and citric acid concentrated solution.
(4), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 100kg wet base of sugar slag and the wet base of 50kg citric acid acid sludge, after adding 50kg Zein powder, 10kg molasses, 25kg clean water, 20kg composite bacteria liquid, the mixing of 0.03kg prozyme, temperature 30��40 DEG C is with under the condition of initial pH:6��8, after first having oxygen, anaerobically fermenting is after 84 hours, the wet base of the obtained 122kg glycolysis slag dregs of rice.
(5), the preparation of livestock and poultry acidifying and glycolysis feed:
After 230kg lactic acid and citric acid concentrated solution, the wet base of the 122kg glycolysis slag dregs of rice are mixed, dry and make 225kg livestock and poultry acidifying and glycolysis feed.
Or after wet to 90kg lactic acid concentrated solution and 100kg glycolysis slag dregs of rice base and 50kg succinic acid solution, 30kg phosphoric acid are mixed, dry and make 150kg livestock and poultry acidifying and glycolysis feed.
Embodiment 9
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
By 60kg crush maize, add after 150kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 210kg band slag saccharified liquid.
In 210kg band slag saccharified liquid, the aspergillus niger strain liquid 15kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 220kg. Through the solid-liquid separation wet base of obtained 50kg citric acid acid sludge and 170kg citric acid fermentation broth.
(2), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg is cracked rice pulverizing, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid. Through the solid-liquid separation obtained 20kg wet base of sugar slag and 280kg without slag saccharified liquid.
At 280kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 300kg. Adding 50kg20% dilution heat of sulfuric acid in 300kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the solid-liquid separation wet base of obtained 20kg lactic acid acid sludge and calcium sulfate wet base, 330kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(3), the preparation of lactic acid and citric acid concentrated solution:
Mixing after 330kg lactic acid fermentation liquid and 170kg citric acid fermentation broth being concentrated respectively again, prepares 140kg lactic acid and citric acid concentrated solution.
(4), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 20kg wet base of sugar slag and the wet base of 50kg citric acid acid sludge, after adding 30kg dregs of beans, 20k wheat bran, 10kg rice bran, 10kg alfalfa meal, 8kg molasses, 60kg clean water, 8kg composite bacteria liquid, the mixing of 0.05kg prozyme, temperature 30��40 DEG C is with under the condition of initial pH:6��8, after first having oxygen, anaerobically fermenting is after 72 hours, the wet base of the obtained 168kg glycolysis slag dregs of rice.
(5), the preparation of livestock and poultry acidifying and glycolysis feed:
After 140kg lactic acid and citric acid concentrated solution, the wet base of the 168kg glycolysis slag dregs of rice are mixed, dry and make 308kg livestock and poultry acidifying and glycolysis feed.
Or after 140kg lactic acid and citric acid concentrated solution, the wet base of the 168kg glycolysis slag dregs of rice, 100kg fumaric acid solution, 30kg phosphoric acid and 50kg molasses, 10kg emulsified fat are mixed, dry and make 290kg livestock and poultry acidifying and glycolysis feed.
Embodiment 10
(1), the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
Again by 120kg crush maize, add after 300kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 420kg band slag saccharified liquid.
Measuring 210kg band slag saccharified liquid, the aspergillus niger strain liquid 15kg of citric acid is produced in access, 28-32 DEG C, under the fermentation natural condition of pH, carry out aerobic fermentation 72 hours, the obtained rough citric acid fermentation broth of 220kg. Through the solid-liquid separation wet base of obtained 50kg citric acid acid sludge and 170kg citric acid fermentation broth.
(2), the preparation of the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
100kg is cracked rice pulverizing, add after 200kg water sizes mixing, add ��-amylase, saccharifying enzyme enzymolysis, obtained 300kg band slag saccharified liquid.Through the solid-liquid separation obtained 20kg wet base of sugar slag and 280kg without slag saccharified liquid.
At 280kg without in slag saccharified liquid, the lactic acid bacteria culturers liquid 20kg of access lactic acid producing, Continuous Flow hydro-oxidation calcium solution, temperature 42-45 DEG C, the substantially constant of fermentation pH:7 when, carry out anaerobically fermenting 48-60 hour, the obtained rough lactic acid fermentation liquid of 300kg. Adding 50kg20% dilution heat of sulfuric acid in 300kg lactic acid fermentation liquid, acidifying generates calcium sulfate and lactic acid. Through the obtained 20kg lactic acid acid sludge of solid-liquid separation and calcium sulfate wet base, 330kg lactic acid fermentation liquid. Wherein, the wet base of lactic acid acid sludge and the wet base of calcium sulfate are abandoned.
(3), the preparation of fumaric acid fermented liquid and the wet base of fumaric acid acid sludge:
Measuring 210kg band slag saccharified liquid again, the strain liquid 20kg of fumaric acid is produced in access, 30-35 DEG C, the substantially constant of fermentation pH:7 when, carry out fermentation 120-144 hour, the obtained rough fumaric acid fermented liquid of 230kg. Through the solid-liquid separation wet base of obtained 80kg fumaric acid acid sludge and 130kg fumaric acid fermented liquid.
(4), the preparation of organic acid concentrated solution:
Mixing after 330kg lactic acid fermentation liquid, 170kg citric acid fermentation broth and 130kg fumaric acid fermented liquid being concentrated respectively again, prepares 175kg organic acid concentrated solution.
(5), the preparation of the wet base of the glycolysis slag dregs of rice:
In the 20kg wet base of sugar slag, the wet base of 50kg citric acid acid sludge and the wet base of 80kg fumaric acid acid sludge, after adding 30kg dregs of beans, 20k wheat bran, 10kg rice bran, 10kg alfalfa meal, 8kg molasses, 40kg clean water, 12kg composite bacteria liquid, the mixing of 0.06kg prozyme, temperature 30��40 DEG C is with under the condition of initial pH:6��8, after first having oxygen, anaerobically fermenting is after 72 hours, the wet base of the obtained 190kg glycolysis slag dregs of rice.
(6), the preparation of livestock and poultry acidifying and glycolysis feed:
After 175kg organic acid concentrated solution, the wet base of the 190kg glycolysis slag dregs of rice are mixed, dry and make 360kg livestock and poultry acidifying and glycolysis feed. Or after 175kg organic acid concentrated solution, the wet base of the 190kg glycolysis slag dregs of rice, 100kg calcium formiate, 100kg acetic acid solution, 30kg phosphoric acid and 50kg molasses, 20kg emulsified fat are mixed, dry and make 452kg livestock and poultry acidifying and glycolysis feed.
One, main active substances content in the livestock and poultry acidifying that prepared by embodiment 1, embodiment 2, embodiment 6 and embodiment 10 and glycolysis feed, sees the following form 1,2,3,4.
The livestock and poultry acidifying of table 1, embodiment 1 preparation and glycolysis feeds product Middle nutrition substances content
| Project | Index | Project | Index | Project | Index |
| Color and luster | Tawny or light yellow | Citric acid/% | ��20.0 | Lemon calcium/% | ��0.5 |
| Moisture/% | ��12.0 | Pfansteihl/% | ��2.0 | Total reducing sugar/% | ��1.5 |
| Crude protein/% | ��18.0 | Fumaric acid/% | - | Oligose/% | ��1.0 |
| Little peptide/% | ��1.0 | Acetic acid/% | - | Total amount of probiotics cfu/g | ��0.03��106 |
| Crude fat/% | ��1.0 | Total organic acids/% | ��22.0 | Water-soluble substances | ��30.0 |
The livestock and poultry acidifying of table 2, embodiment 2 preparation and glycolysis feeds product Middle nutrition substances content
| Project | Index | Project | Index | Project | Index |
| Color and luster | Tawny or light yellow | Pfansteihl/% | ��20.0 | Calcium lactate/% | ��0.5 |
| Moisture/% | ��12.0 | Citric acid/% | - | Total reducing sugar/% | ��1.5 |
| Crude protein/% | ��18.0 | Fumaric acid/% | - | Oligose/% | ��1.0 |
| Little peptide/% | ��1.0 | Acetic acid/% | - | Total amount of probiotics cfu/g | ��0.03��106 |
| Crude fat/% | ��1.0 | Total organic acids/% | ��20.0 | Water-soluble substances | ��30.0 |
The livestock and poultry acidifying of table 3, embodiment 6 preparation and glycolysis feeds product Middle nutrition substances content
The livestock and poultry acidifying of table 4, embodiment 10 preparation and glycolysis feeds product Middle nutrition substances content
| Project | Index | Project | Index | Project | Index |
| Color and luster | Tawny or light yellow | Pfansteihl/% | ��10.0 | Calcium lactate/% | ��0.2 |
| Moisture/% | ��12.0 | Citric acid/% | ��5.0 | Total reducing sugar/% | ��1.5 |
| Crude protein/% | ��10.0 | Fumaric acid/% | ��3.0 | Oligose/% | ��1.0 |
| Little peptide/% | ��0.5 | Acetic acid/% | - | Total amount of probiotics cfu/g | ��0.03��106 |
| Crude fat/% | ��2.0 | Total organic acids/% | ��18.0 | Water-soluble substances | ��20.0 |
Two, the livestock and poultry acidifying of embodiment 1 preparation and the weanling pig effect of feeding of glycolysis feed.
The livestock and poultry acidifying prepared for discussion embodiment 1 and glycolysis feed are to the feeding effect of weanling pig, choosing age on the 23rd Du to grow up three way cross sodium selenite 500, close by body weight, parity, the principle of sex half and half is divided into 5 groups at random, often organizing 10 repetitions, each repeats 10 pigs. Control group does not add souring agent. Tartaric acid fodder additives group adds tartaric acid fodder additives 6kg/ ton (it ferments to prepare according to " preparation method of a kind of tartaric acid fodder additives " (number of patent application: CN201510424595.8) and produces). Test group is pressed 10kg/ ton, 20kg/ ton and 30kg/ ton respectively and is added livestock and poultry acidifying and glycolysis feed, makes weaning pigs. Each group feed protein is close with energy. Piglet wean in 23 days, 30 days trial periods. Test-results is as follows:
The livestock and poultry acidifying of table 5, embodiment 1 preparation and glycolysis feed are on the impact of weanling pig production performance
After daily ration adds 10kg/ ton, 20kg/ ton and 30kg/ ton livestock and poultry acidifying and glycolysis feed, the day weight gain of weanling pig improves 3.28%, 5.44% and 6.45% than tartaric acid fodder additives group (that is: adding tartaric acid fodder additives) respectively, diarrhea rate reduces by 50.00%, 58.33% and 75.00% than tartaric acid fodder additives group respectively, feedstuff-meat ratio reduces by 0.00%, 0070% and 1.40% than tartaric acid fodder additives group respectively, and food consumption improves 3.28%, 4.71% and 4.97% than tartaric acid fodder additives group respectively. Result shows: the feeding effect of livestock and poultry acidifying and glycolysis feed is better than tartaric acid fodder additives.
After daily ration adds 10kg/ ton, 20kg/ ton and 30kg/ ton livestock and poultry acidifying and glycolysis feed, the day weight gain of weanling pig improves 10.04%, 12.34% and 13.42% than control group respectively, diarrhea rate reduces by 89.09%, 90.91% and 94.55% than control group respectively, feedstuff-meat ratio reduces by 2.10%, 2.82% and 3.55% than control group respectively, and food consumption improves 7.78%, 9.26% and 9.54% than control group respectively. Result shows: livestock and poultry acidifying and glycolysis feed can significantly improve the production performance of weanling pig.
Three, the livestock and poultry acidifying of embodiment 2 preparation and the growing swine effect of feeding of glycolysis feed.
The livestock and poultry acidifying prepared for discussion embodiment 2 and glycolysis feed are to the feeding effect of growing swine, select the Du Chang great three way cross piglet 84 (piglet age on the 59th and 60) of body weight close (body weight 18.9 �� 0.85kg), it is divided into test group and control group at random. Test group 42 (sow 22, boar 20), control group 42 (sow 19, boar 23). Two groups of pig weight close (significance analysis is not remarkable), all establish 7 repetitions for each group, and each repeats 6.
Test group is all identical with control group piglet basal diet trophic level, is powder, and control group is not containing livestock and poultry acidifying and glycolysis feed, and test group adds livestock and poultry acidifying and glycolysis feed 20kg/ ton.
The test prerun phase: test prerun from 28 days September in 2015,10 days prerun phases, carrying out the work such as prevention, inoculation, expelling parasite, prerun enters after terminating and just tries.
Test just try the phase in advance: test from October 7th, 2015 formally, on December 8th, 2015 formally terminates, and lasts 54 days.
After adding livestock and poultry acidifying and glycolysis feed 20kg/ ton in daily ration, test group 42 piglets are the heavy 38.06kg in end on average, and control group is the heavy 35.71kg in end on average, test group manys 2.35kg than control group, it is to increase 6.58% (P < 0.05);Test group average weight gain 19.24kg, control group average weight gain 16.66kg, test group manys 2.58kg than control group, it is to increase 15.49% (P < 0.01); Test group average daily gain 356.30g, control group average daily gain 308.52g, test group manys 47.78g than control group, it is to increase 15.49% (P < 0.01). Refer to table 5.
The livestock and poultry acidifying of table 6, embodiment 2 preparation and glycolysis feed are on the impact of growing swine day weight gain
| Group | Head number | Test number of days | Begin weight/head/kg | End weight/head kg | Total augment weight/head/kg | Day weight gain/head/g |
| Control group | 42 | 54 | 19.05 | 35.71��1.69 | 16.66��1.04 | 308.52��64 |
| Test group | 42 | 54 | 18.82 | 38.06��2.85* | 19.24��1.67** | 356.30��111** |
Note:**Represent the significance of difference (P < 0.01) in row,*Represent the significance of difference (P < 0.05) in row.
After daily ration adds livestock and poultry acidifying and glycolysis feed 20kg/ ton, test group every food consumption 45.21kg, control group every food consumption 40.32kg, test group 40.89kg. more than control group, food consumption adds 12.13% (P<0.05); Test group feed conversion rate 2.35, fewer than control group 2.42 0.07, test group reduces 2.89% (P>0.05) than control group. Refer to table 6.
The livestock and poultry acidifying of table 7, embodiment 2 preparation and glycolysis feed are on the impact of growing swine feed conversion rate
| Group | Weightening finish/head/kg | Food consumption/head/kg | Feed conversion rate | Feed cost/unit | Cost/unit/1kg weightening finish |
| Control group | 16.66 | 40.32��1.23 | 2.42��0.49 | 60.48 | 3.63 |
| Test group | 19.24 | 45.21��2.35* | 2.35��0.83 | 67.82 | 3.52 |
Note: by there and then feedstuff raw material calculation of price, feed 1.508 yuan/kg.
Four, the livestock and poultry acidifying of embodiment 6 preparation and the broiler chicken effect of feeding of glycolysis feed.
For inquiring into livestock and poultry acidifying and glycolysis feed prepared by embodiment 6 to the feeding effect of broiler chicken, choose AA broiler chicken 800 age on the 1st, it is divided into 4 groups at random by the principle of body weight, sex half and half, often organize 10 repetition, each repetition 20. Add by 5kg/ ton, 10kg/ ton and 15kg/ ton, make broiler chicken material, the daily ration of control group does not add livestock and poultry acidifying and glycolysis feed. Each group feed protein is close with energy. 42 days trial periods. Test-results is as follows:
After broiler chicken material adds 5kg/ ton, 10kg/ ton and 15kg/ ton livestock and poultry acidifying and glycolysis feed, broiler chicken day weight gain improves 8.54%, 11.44% and 12.58% (P < 0.01) than control group respectively, food consumption improves 6.52%, 8.48% and 11.52% (P < 0.05 or P < 0.01) than control group respectively, and feedstuff-meat ratio reduces by 4.08%, 5.10% and 4.59 (P < 0.05) than control group respectively. Result shows: livestock and poultry acidifying and glycolysis feed can significantly improve the production performance of broiler chicken.
Livestock and poultry acidifying prepared by table 8. embodiment 6 and glycolysis feed are on the impact of meat chicken production performance
Five, the livestock and poultry acidifying of embodiment 10 preparation and the laying hen effect of feeding of glycolysis feed.
For inquiring into livestock and poultry acidifying and glycolysis feed prepared by embodiment 10 to the feeding effect of laying hen, choose egg-laying peak Roman egghen 800 in 34 weeks age, it is divided into 4 groups at random, often organize 10 repetitions, each repetition 20. Add by 5kg/ ton, 10kg/ ton and 15kg/ ton, make laying hen material, the daily ration of control group does not add livestock and poultry acidifying and glycolysis feed. Each group feed protein is close with energy. 28 days trial periods. Test-results is as follows:
After Roman egghen material adds 5kg/ ton, 10kg/ ton and 15kg/ ton livestock and poultry acidifying and glycolysis feed, 37 week age, laying rate improved 3.69%, 6.92% and 7.72% (P>0.05 or P<0.05) than control group respectively, and 38 week age, laying rate improved 4.50%, 8.97% and 9.35% (P>0.05 or P<0.05) than control group respectively.Result shows: livestock and poultry acidifying and glycolysis feed can significantly improve the egg laying performance of laying hen.
Livestock and poultry acidifying prepared by table 9. embodiment 10 and glycolysis feed are on the impact of Roman egghen egg laying performance
| Age in week | Control group | 5kg/ ton | 10kg/ ton | 15kg/ ton |
| 35 | 82.43��0.025 | 83.49��0.018 | 84.98��0.071 | 85.11��0.015 |
| 36 | 80.08��0.032 | 82.02��0.021 | 84.01��0.024 | 84.55��0.028 |
| 37 | 78.25��0.020b | 81.40��0.066ab | 83.93��0.050a | 84.56��0.035a |
| 38 | 76.92��0.012b | 80.38��0.044ab | 83.82��0.029a | 84.11��0.048a |
Above content is only citing made for the present invention and explanation; described specific embodiment is made various amendment or supplements or adopt similar mode to substitute by affiliated those skilled in the art; as long as not deviateing the design of invention or surmounting this scope as defined in the claims, protection scope of the present invention all should be belonged to.
Claims (10)
1. the preparation method of a livestock and poultry acidifying and glycolysis feed, it is characterised in that, comprise the following steps:
1., the preparation of the wet base of sugar slag, citric acid fermentation broth and the wet base of citric acid acid sludge:
To pulverize containing starch product, and add water and size mixing, and add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access produces citric acid strain fermentation some hours, through the solid-liquid separation wet base of obtained citric acid acid sludge and citric acid fermentation broth;
Maybe will pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; Citric acid strain fermentation is produced after some hours, directly obtained citric acid fermentation broth without access in slag saccharified liquid;
2., the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; Without slag saccharified liquid accesses lactic acid producing bacterial classification, ferment some hours when Continuous Flow hydro-oxidation calcium solution, then add after dilute sulphuric acid carries out acidifying, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
Maybe will pulverize containing starch product, and add water and size mixing, add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access lactic acid producing bacterial classification, ferment some hours when flowing hydro-oxidation calcium solution, add after dilute sulphuric acid carries out acidifying again, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
3., the preparation of the wet base of sugar slag, other organic acid fermentation liquid and the wet base of other organic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; In without slag saccharified liquid, access one or more other organic acid strain fermentations some hours, obtain the wet base of other organic acid acid sludge and other organic acid fermentation liquid through solid-liquid separation;
Maybe will pulverize containing starch product, and add water and size mixing, add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly one or more other organic acid strain fermentations of access access some hours, obtain the wet base of other organic acid acid sludge and other organic acid fermentation liquid through solid-liquid separation;
Wherein, other organic acid refers to the organic acid except lactic acid, citric acid;
4., the preparation of fermentation source organic acid concentrated solution:
After one in lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid directly being concentrated, prepare fermentation source organic acid concentrated solution;
Or by concentrated after more than one mixing in lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid, or mixing after concentrating respectively, prepare fermentation source organic acid concentrated solution;
5., the preparation of the wet base of the glycolysis slag dregs of rice:
Enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge, the wet base of other organic acid acid sludge are mixed with composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
Or enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge, the wet base of other organic acid acid sludge are mixed with feedstuff raw material, clean water, composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
6., the preparation of livestock and poultry acidifying and glycolysis feed:
After wet to fermentation source organic acid concentrated solution and glycolysis slag dregs of rice base is mixed, direct production livestock and poultry acidifying and the wet base of glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively;
Or after fermentation source organic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed with one or more in commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat, the wet base of direct production livestock and poultry acidifying and glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively.
2. the preparation method of a kind of livestock and poultry acidifying according to claim 1 and glycolysis feed, it is characterised in that, comprise the following steps:
1., the preparation of citric acid fermentation broth and the wet base of citric acid acid sludge:
To pulverize containing starch product, and add water and size mixing, and add amylase enzymolysis liquefying-saccharifying, after obtained band slag saccharified liquid, directly access produces citric acid strain fermentation some hours, through the solid-liquid separation wet base of obtained citric acid acid sludge and citric acid fermentation broth;
2., the preparation of the wet base of sugar slag, lactic acid fermentation liquid and the wet base of lactic acid acid sludge:
To pulverize containing starch product, and add water and size mixing, after adding amylase enzymolysis liquefying-saccharifying, the wet base of solid-liquid separation obtained sugar slag and without slag saccharified liquid; Without slag saccharified liquid accesses lactic acid producing bacterial classification, ferment some hours when Continuous Flow hydro-oxidation calcium solution, then add after dilute sulphuric acid carries out acidifying, through the solid-liquid separation wet base of obtained lactic acid acid sludge, calcium sulfate and lactic acid fermentation liquid;
3., the preparation of fermentation source organic acid concentrated solution:
After one in lactic acid fermentation liquid, citric acid fermentation broth directly being concentrated, prepare fermentation source organic acid concentrated solution;
Or by concentrated after more than one mixing in lactic acid fermentation liquid, citric acid fermentation broth, or mixing after concentrating respectively, prepare fermentation source organic acid concentrated solution;
4., the preparation of the wet base of the glycolysis slag dregs of rice:
Enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge are mixed with composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
Or enzymatic hydrolysis and fermentation after one or more in wet for sugar slag base, the wet base of citric acid acid sludge, the wet base of lactic acid acid sludge are mixed with feedstuff raw material, clean water, composite bacteria liquid, prozyme, the wet base of the obtained glycolysis slag dregs of rice;
5., the preparation of livestock and poultry acidifying and glycolysis feed:
After citric acid and lactic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed, direct production livestock and poultry acidifying and the wet base of glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively;
Or after citric acid and lactic acid concentrated solution, the wet base of the glycolysis slag dregs of rice are mixed with one or more in commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat, the wet base of direct production livestock and poultry acidifying and glycolysis feed, or mixing post-drying or be mixed and made into livestock and poultry acidifying and the dry base of glycolysis feed after drying respectively.
3. the preparation method of a kind of livestock and poultry acidifying according to claim 1 and glycolysis feed, it is characterised in that,
Other organic acid fermentation liquid described is a kind of fermented liquid of organic acid or multiple fermented liquid in the Ministry of Agriculture of country fodder additives catalogue, a kind of fermented liquid being mainly in fumaric acid, oxysuccinic acid, a-ketoglutaric acid, succsinic acid, tartrate, acetic acid, propionic acid, butyric acid, xitix, Whitfield's ointment, gibberic acid, kojic acid, gluconic acid, propylenedicarboxylic acid or multiple fermented liquid;
Described feedstuff raw material is one or more of feedstuff raw material in country's Ministry of Agriculture feedstuff raw material catalogue, it is mainly dregs of beans, cotton dregs, rapeseed meal, peanut meal, fish meal, blood meal, plasma proteins, hyperglobulinemia, milk powder, lactose, whey powder, wheat gluten flour, Zein powder, rice protein powder, DDGS, soybean, pea, red bean, corn, paddy, crack rice, wheat, wheat bran, rice bran, flour, oat, barley, Ipomoea batatas, soybean protein isolate, soybean protein concentrate, pasture powder, herbal medicine, fruit and vegetable, sugar molasse syrup and food-processing industry byproduct, or above-mentioned feedstuff raw material dilated product in one or more,
Described commercially available feeding non-fermented source organic acid be in the country's Ministry of Agriculture fodder additives catalogue non-fermented organic acid one or more, be mainly in formic acid, phenylformic acid, tartrate, fumaric acid, Sorbic Acid one or more;
Described commercially available feeding mineral acid is one or more of mineral acid in country's Ministry of Agriculture fodder additives catalogue, be mainly in phosphoric acid, hydrochloric acid, sulfuric acid one or more;
Described organic acid salt is one or more of organic acid salt in country's Ministry of Agriculture fodder additives catalogue, be mainly in calcium formiate, Sodium Propionate, calcium propionate, Sodium propanecarboxylate, Sodium Benzoate, sodium sorbate, potassium sorbate, Tripotassium Citrate, Trisodium Citrate, citrate of lime one or more;
Described sugar molasse syrup is one or more of sugar molasse syrup in country's Ministry of Agriculture feedstuff raw material catalogue, be mainly in sucrose, glucose, fructose, glucose syrup, maltodextrin slurry/powder, high fructose syrup/powder, malt syrup/powder, Sugar cane molasses, beet sirup one or more;
Described liquid fat is one or more of liquid fat in country's Ministry of Agriculture feedstuff raw material catalogue, be mainly in soybean oil and oil in water emulsion, Semen Maydis oil and oil in water emulsion, plam oil and oil in water emulsion, Rice pollard oil and oil in water emulsion, Oleum Cocois and oil in water emulsion, fish oil and animal oil and oil in water emulsion, peanut oil and oil in water emulsion, rapeseed oil and oil in water emulsion, tea oil and oil in water emulsion thereof one or more.
4. the preparation method of a kind of livestock and poultry acidifying according to claim 1 or 2 or 3 and glycolysis feed, it is characterised in that,
Other organic acid fermentation liquid described be fumaric acid, succsinic acid, tartrate, acetic acid, propionic acid, butyric acid, in a kind of fermented liquid or multiple fermented liquid;
Described feedstuff raw material is dregs of beans, cotton dregs, Zein powder, rice protein powder, DDGS, wheat bran, rice bran, pasture powder, herbal medicine, fruit and vegetable, sugar molasse syrup and food-processing industry byproduct, or above-mentioned feedstuff raw material dilated product in one or more;
Described commercially available feeding non-fermented source organic acid is one or more in formic acid, phenylformic acid, fumaric acid;
Described commercially available feeding mineral acid is phosphoric acid;
Described organic acid salt is one or more in calcium formiate, Sodium propanecarboxylate, Trisodium Citrate, citrate of lime;
Described sugar molasse syrup is one or more in sucrose, glucose, glucose syrup, Sugar cane molasses, beet sirup;
Described liquid fat is one or more in soybean oil and oil in water emulsion, Semen Maydis oil and oil in water emulsion, plam oil and oil in water emulsion, Rice pollard oil and oil in water emulsion, Oleum Cocois and oil in water emulsion, fish oil and animal oil and oil in water emulsion thereof.
5. the preparation method of a kind of livestock and poultry acidifying according to claim 1 and glycolysis feed, it is characterised in that, step 4. in the preparation condition of fermentation source organic acid concentrated solution as follows:
(1) when, preparing citric acid fermentation broth: produce citric acid waste residue strain fermentation temperature: 10��40 DEG C;Fermentation pH: nature; Fermented type: aerobic fermentation; Fermentation time: 6-216 hour;
(2) when, preparing lactic acid fermentation liquid: lactic acid producing bacterial classification leavening temperature: 20��60 DEG C; Fermentation pH:5��9; Fermented type: anaerobically fermenting; Fermentation time: 6-168 hour;
(3), the weight ratio of lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid is 100:0��1000:0��500.
6. the preparation method of a kind of livestock and poultry acidifying and glycolysis feed according to claim 1 or 5, it is characterised in that, step 4. in the preparation condition of fermentation source organic acid concentrated solution as follows:
(1) when, preparing citric acid fermentation broth: produce citric acid waste residue strain fermentation temperature: 28��32 DEG C; Fermentation pH: nature; Fermented type: aerobic fermentation; Fermentation time: 60-72 hour;
(2) when, preparing lactic acid fermentation liquid: lactic acid producing bacterial classification leavening temperature: 40��50 DEG C; Fermentation pH:6��8; Fermented type: anaerobically fermenting; Fermentation time: 48-60 hour;
(3), the weight ratio of lactic acid fermentation liquid, citric acid fermentation broth and other organic acid fermentation liquid is 100:50��80:20��40.
7. the preparation method of a kind of livestock and poultry acidifying according to claim 1 and glycolysis feed, it is characterised in that, step 5. in the preparation condition of the wet base of the glycolysis slag dregs of rice as follows:
(1), the weight ratio of the wet base of sugar slag, the wet base of citric acid acid sludge, the wet base of other organic acid acid sludge and feedstuff raw material, clean water, composite bacteria liquid, prozyme is 100:0��500:0��300:0��3000:0��2000:0.5��50:0.0001��10;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast strain liquid: the number of viable of Bacillus licheniformis kind liquid is than being 100:0��200:0��100;
(3), the commercial protein enzyme in prozyme: zytase: dextranase: mannase: the weight ratio of polygalacturonase is 100:0��1000:0��1000:0��1000:0��1000;
(4), enzymatic hydrolysis and fermentation temperature: 10��60 DEG C, first there is anaerase hydrolysis and fermentation 12-240 hour after oxygenase hydrolysis and fermentation initial pH:4��9 of enzymatic hydrolysis and fermentation.
8. the preparation method of a kind of livestock and poultry acidifying according to claim 1 or 7 and glycolysis feed, it is characterised in that, step 5. in the preparation condition of the wet base of the glycolysis slag dregs of rice as follows:
(1), the weight ratio of the wet base of sugar slag, the wet base of citric acid acid sludge, the wet base of organic acid acid sludge and feedstuff raw material, clean water, composite bacteria liquid, prozyme is 100:0��80:0��40:200��800:0��200:10��20:0.005��2;
(2), the lactic acid bacteria culturers liquid in composite bacteria liquid: yeast strain liquid: the number of viable of Bacillus licheniformis kind liquid is than being 100:10��30:5��10;
(3), the commercial protein enzyme in prozyme: zytase: dextranase: mannase: the weight ratio of polygalacturonase is 100:60��80:60��80:60��80:30��50;
(4), enzymatic hydrolysis and fermentation temperature: 30��40 DEG C, first there is anaerase hydrolysis and fermentation 72-96 hour after oxygenase hydrolysis and fermentation initial pH:6��8 of enzymatic hydrolysis and fermentation.
9. the preparation method of a kind of livestock and poultry acidifying according to claim 1 and glycolysis feed, it is characterised in that, step 6. in the preparation condition of livestock and poultry acidifying and glycolysis feed as follows:
The weight ratio of the wet base of fermentation source organic acid concentrated solution, the glycolysis slag dregs of rice and commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat is 100:0��2000:0��300:0��300:0��300:0��500:0��500.
10. the preparation method of a kind of livestock and poultry acidifying according to claim 1 or 8 and glycolysis feed, it is characterised in that, step 6. in the preparation condition of livestock and poultry acidifying and glycolysis feed as follows:
The weight ratio of the wet base of fermentation source organic acid concentrated solution, the glycolysis slag dregs of rice and commercially available feeding non-fermented source organic acid, commercially available feeding mineral acid, organic acid salt, sugar molasse syrup, liquid fat is 100:200��800:20��40:10��20:10��20:50��100:10��20.
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Application publication date: 20160608 |