CN101209088A - Microorganism polyzyme additive agent for improving pigling growth and development - Google Patents
Microorganism polyzyme additive agent for improving pigling growth and development Download PDFInfo
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Abstract
The invention relates to a microorganism multienzyme additive for promoting the growth and development of baby pigs, which is characterized in that the additive is formed by composite synchronizing solid fermentation of a composite strain stock solution, carbon source, nitrogen source, inorganic salt and enzyme revulsant. A manufacturing method is that all the strains adopt a composite inducing technology to carry through filtering; a method of synchronizing liquid culture and solid composite culture is adopted to lead all the strains to be cultivated in the same culture medium; the total number of live bacterium reaches 3 billion/g and simultaneously a fermented product is kept completely. The product comprises massive physiological metabolism substances like active enzyme, small peptide, oxidation resistance substances, FAA, vitamins, etc. A specific type for feed preparing particles carries through a double-layer microcapsule peridium treatment on the strains that can not resist heat. The invention has the advantages of promoting the digestive absorption on the feed by the baby pigs, promoting the compound of organism protein and full utilization, increasing the disease resistance and immunological competence of the baby pigs, improving the feed switching rate and the production capacity of cultivated animals, reducing the pollution to the environment caused by animal dejecta. The invention is a green and safe agrotechnical product.
Description
Technical field
The present invention relates to a kind of microorganism polyzyme additive agent that promotes piglet growth and growth, relate in particular to a kind of technical products that utilizes multiple microbial fermentation to produce multienzyme, can promote that piglet digests and assimilates ability, improve the microbe additive of immunity and cold-resistant copingability and survival rate, belong to the agricultural products technical field.
Background technology
At present, disclosed Chinese patent, publication number is CN98109067.2, be entitled as additive for feed for piglets, it is characterized in that: contain purslane, fructus crataegi cuneatae, content and be respectively 15-25% and 10-20%, also contain single mixture of planting acid or multiple acid that 30-50% can be feeding simultaneously, other is equipped with various flavor enhancements, be aided with adsorbent and glidant, mixing and stirring after the various composition preliminary treatment is got product.This additive is at the still immature characteristics of piglet digestive organs, the additive that provides a kind of suitable pig starter feed to cooperate. this biology feed additive bacterial classification is a kind of Chinese herb feed additive in fact, digestive ferment and anti-immune substance that piglet grows to be needed can't be provided, and its using value is not high.
Piglet grows and is related to the key that pork pig grows throughout one's life, because piglet has body heat regulation incomcopetence, resistance to cold stress is poor, digestive organs are unsound, glandula digestive function imperfection, shortage congenital immunity power, characteristics such as fall ill easily, piglet survival rate under general raising condition is not high, and it is comparatively general that diseases such as dysentery, yellow dysentery, dysentery characterized by white mucous stool, indigestion take place, and reduced piglet survival ratio.
Summary of the invention
The purpose of this invention is to provide a kind of beneficial bacterium, to count height, enzymatic productivity strong and have an additive of materials such as various active enzyme and little peptide.After interpolation is fed, can in the piglet alimentary canal, form the optimum ecological environment of animal body, the microbial enzyme additive that contains a large amount of activity simultaneously can remedy the deficiency that the geneogenous glandula digestive of piglet alimentary canal is grown and secreted, and significantly improves the feed conversion rate of piglet.The metabolism antibiotic such as bacitracin that contain simultaneously some in the additive can significantly improve the human body immunocompetence of piglet, prevent the generation of common infectious disease of the digestive tract, improve the survival rate of piglet.
For realizing above purpose, technical scheme of the present invention provides a kind of microorganism polyzyme additive agent that promotes piglet growth and growth, it is characterized in that, form: streptococcus lactis 18-20%, saccharomyces cerevisiae 20-23%, bacillus subtilis 13-16%, Bifidobacterium 23-26%, Lactobacillus casei 15-26% by following percentage by weight raw material; It adopts synchronous compound culture method as method, and all bacterial classifications are cultivated in same culture medium, and it comprises the following steps:
The first step, cultivation bacterial classification
(1) adopting power is that the uviol lamp of 15W adds respectively in the aseptic flat board that fills starch culture-medium each 3ml of bacteria suspension of the streptococcus lactis of preservation, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, Lactobacillus casei at 30cm place, shine and cultivated counting in 1-3 minute, the transparent circle of observation on starch culture-medium chosen transparent circle diameter and colony diameter and cultivated to the test tube slant than the bacterium colony culture transferring of (HC)＞1.5;
(2) have and place nitrosoguanidine crystallization 0.5g on the 6mm starch culture-medium flat board of bacterial classification, flat board is inverted in 37 ℃ constant temperature culture 24 hours, observe nitrosoguanidine inhibition zone size on every side, choose transparent circle diameter and colony diameter and to the test tube slant, do the production production of hybrid seeds than the bacterium colony culture transferring of (HC)＞1.5;
Second step, the production production of hybrid seeds:
The streptococcus lactis, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, the Lactobacillus casei that filter out are transferred respectively in the eggplant bottle that nutrient agar is housed, under 35-40 ℃ of temperature, cultivated 45-50 hour, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white, put into 2-6 ℃ refrigerator then and preserve;
The 3rd step, bacterial classification liquid at high speed are cultivated
It is middle cultivation that all bacterial classifications that step 2 is cultivated are placed in same fluid nutrient medium by weight percentage, the ph value is to 6.9-7.4, putting into fermentation tank cultivates, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, throughput 1: 0.6, fermentation time is 18-20 hour, obtains composite bacteria agent capable;
When being used for feed granulating, need to add the gelatin of 1% Arabic gum and 1% in the microbial inoculum, wrap processed heat labile Bacillus acidi lactici, Bifidobacterium;
The solid of the 4th step, composite bacteria agent capable is cultivated synchronously
Ferment inserting in the vapor tight tank after even mixing of composite bacteria agent capable 15% ratio adding solid medium, fermentation temperature is 30-35 ℃, fermentation time is 8-10 days, carry out the detection of moisture and total viable count in the sweat, fermentation ends when total viable count 〉=3,000,000,000/g, the product after the fermentation ends, put into boiling drier and carry out drying, moisture is controlled in 12%, and dried product is pulverized, and is piglet multienzyme feed addictive finished product.
Described solid medium is at least two kinds of mixtures in bean cake powder, urea, ammonium sulfate, blue or green chaff, dusty yeast, corn steep liquor, corn flour, molasses, magnesium phosphate, magnesium sulfate, enzyme inducer, the sterilized water, and its weight percent proportioning is: blue or green chaff 38-40%, bean cake powder 5-7%, corn flour 8-10%, molasses 15-18% dusty yeast 0.5-0.8%,, magnesium phosphate 0.2-0.3%, enzyme inducer 0.05-0.1%, sterilized water 23.8-33.25%.
Described enzyme inducer is made up of wood sugar and sophorose, and its percentage by weight is: wood sugar 50-55%, sophorose 45-50%.
The present invention carries out bilayer bag quilt to thermo-labile bacterial classification with Arabic gum, gelatin feed when granulating; Described carbon source is one of clear chaff, cornstarch, molasses, corn flour or their mixture, and wherein starch is for keeping the active most important of enzyme when the feed granulating; Described nitrogenous source is one of soya-bean cake powder, peanut cake, dusty yeast, corn steep liquor or their mixture, and nitrogenous source decomposes under the organized enzyme effect that is produced by bacterial metabolism as nutriment, can form little peptide and free amino acid; Described inorganic salts are one of ammonium salt, nitrate, phosphate or their mixture; The wood sophorose is mainly used in the output that improves organized enzyme.
The present invention adopts and is mixed with organic nutrient substance by the bacterial classification that filters out in the multiple animal intestinal, make by microbial fermentation technology, it contains various active enzyme, little peptide, free amino acid, monomeric protein, polyphenoils, vitamin and antibacterial material, and the quantity of beneficial bacterium viable count has reached 3,000,000,000/gram; Acid protease 6000u/g, zytase 8000u/g, 1,4 beta-glucanase 7000u/g, pectase 8500u/g, phytase 650u/g; Active small peptide content is greater than 23%.
Piglet is exactly that to make piglet obtain the highest survival rate, maximum weight of weaning litter, individual size evenly neat in the target of raising the nurture phase, lay a good foundation for cultivating boar and commodity pork pig later on, raising nurture phase piglet can be divided into it suckling pig cultivation stage that relies on the breast milk life and the weanling pig breeding phase that is carried out the transition to the free choice feeding feed, lived on one's own life by breast milk.Because the growth temperature regulative mechanism of piglet is unsound, resistance to cold stress is poor, digestive organs are unsound, glandula digestive function imperfection, lack congenital immunity power, characteristics such as fall ill easily, piglet survival rate under general raising condition is not high, dysentery takes place, yellow dysentery, dysentery characterized by white mucous stool, diseases such as indigestion are comparatively general, make the raising of piglet become a difficult ring in the pork pig raising, the gastro-intestinal digestion enzyme is animal intestinal digestion and the main matter that absorbs nutritional labeling, because endogenous digestive ferment hyposecretion, cause the pig starter feed conversion ratio low, easily suffer from " prohibiting mouth ", hypoglycemia, body weight increases slowly, flu when serious, diarrhoea, even it is dead.
The flora that the various kinds of cell of surviving in the enteron aisle of normal livestock and poultry constitutes, after the beneficial microbe in the piglet polyzyme additive agent enters the enteron aisle of livestock and poultry and aquatic products, in conjunction with original beneficial bacterium, form the superior microorganism flora rapidly, suppressed growth of harmful intestinal tract bacteria, beneficial microbe is wherein combined closely by LTA and intestinal epithelial cell around intestinal walls, forms the mycoderm barrier, stops the field planting of harmful bacterium; Complex microorganism can also produce lactolin, antibacterial material, acidophilin,, bacitracin etc., suppress breeding, field planting and the absorption of harmful bacterium such as Escherichia coli, salmonella in enteron aisle, in and toxic product, suppress the synthetic of ammonia and amine, promote the height respond of gut-associated lymphoid tissue, immune organ is grown speed, immune system is ripe early, fast, and the quantity of T, bone-marrow-derived lymphocyte increases, body fluid and the cellular immune level of animal are improved, strengthen body resistance against diseases.
AMS contained in the piglet polyzyme additive agent claims starch 1 again, the 4-dextromase, can cut the α-1 of starch chain inside, the 4-glycosidic bond is hydrolyzed to starch maltose, contains the oligosaccharides of 6 glucose units and has the oligosaccharides of side chain, mainly given birth to by producing bacillus subtilis.Protease can be hydrolyzed into little peptide or amino acid with the big molecular polypeptide of forming albumen in the feed; Cellulase, pectase can be abolished plant cell wall, and cellular content is fully discharged, by the stomach animal intestinal is absorbed; 1,4 beta-glucanase, zytase energy hydrolysis water dissolubility 1,4 beta-glucanase, xylan, effectively reduce chyme viscosity in the animal intestinal, help the abundant and chyme mixing of endogenous digestive juice, fully digestion, reduce livestock and poultry diarrhea, be beneficial to utilization rate and energy that nutraceutical absorption improves feed, reduce the material anharmonic ratio; Phytase can transform to orthophosphates, inositol and inositol derivative by the catalysis phytic acid, phosphorus hydrolysis in the phytic acid is discharged, make phosphorus wherein must think animal use, can reduce like this and add inorganic acid salt, reduce the discharging of ight soil phosphorus, alleviate pollution to environment, the piglet polyzyme additive agent also can reduce the generation of ammonia and other corrupt substance, ammonia amount in intestinal contents, ight soil and the quiet portal vein is descended, first cresols Yin once odorant such as increased with 3-methyl Yin reduce in the intestinal contents, thereby reduce the foul smell that ight soil produces, reach deodorizing effect.
A large amount of little peptide materials can be eliminated the absorption competition of free amino acid in the piglet polyzyme additive agent, accelerate the synthetic of animal body internal protein, improve the deposition efficiency of protein, promote the absorption and the utilization of mineral matter element, and some peptide has antioxidation, by the decomposition of removing heavy metal ion and promoting to become the peroxide of free radical, reduce the peroxide content of automatic oxidation rate and fat, can significantly improve the production performance and the immunocompetence of letting animals feed.
The piglet polyzyme additive agent adopts carbon source, nitrogenous source, inorganic salts and enzyme inducer to cultivate raw material as bacterial classification, they are important component parts of microbial cell structure and enzyme, also can keep the activity of enzyme, can promote the growth of microorganism breeding, the generation of stimulating activity enzyme and little peptide.
Advantage of the present invention is:
1. adopt the co-induction breeding technique, the yield of enzyme of bacterial classification and activity are improved greatly,, promote animal digesting and assimilating feed for piglet provides the necessary enteron aisle digestive ferment that grows;
2. contain in the product and culture necessary alimentary canal bacterial classification in the domestic animals, can effectively set up the intestinal microecology system of piglet, improve digestion and the immunocompetence of piglet;
3. sufficient little peptide material is provided, promotes the abundant absorption of nutriments such as body protein, significantly improve production performance;
4. effectively decompose the big molecular nutrition material that some animals are difficult for absorption, improve the utilization rate of the nutritive value of inferior feed ingredient;
5, be rich in organized enzyme and immune peptide, both can provide exogenous enzymes, eliminate in the diet anti-nutrition because of, can strengthen the natural immunity ability of cultured birds, prevention common transmittable disease again;
6, improve food conversion ratio, alleviate the pollution of ight soil environment;
7, alternative is the antibiotic use of purpose with prevention and growth promotion, produces the organic farm products of green, safety.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail:
All bacterial classifications that adopt all come from the microorganism fungus kind of stipulating in the feed interpolation of the Ministry of Agriculture's 658 bulletins, are provided by DSMZ of the Ministry of Agriculture, utilize the complex mutation breeding technique that each bacterial classification is carried out preferred mutation breeding.
Embodiment 1:
The first step, cultivation bacterial classification, utilize the complex mutation breeding technique that each bacterial classification is carried out preferably carrying out the fermenting and producing performance test:
(1) adopting power is that the uviol lamp of 15W adds respectively in the aseptic flat board that fills starch culture-medium each 3ml of bacteria suspension of the streptococcus lactis of preservation, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, Lactobacillus casei at 30cm place, shine and cultivated counting in 1-3 minute, the transparent circle of observation on starch culture-medium chosen transparent circle diameter and colony diameter and cultivated to the test tube slant than the bacterium colony culture transferring of (HC)＞1.5;
Starch culture-medium: peptone 10g, sodium chloride 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g.
(2) have and place nitrosoguanidine crystallization 0.5g on the 6mm starch culture-medium flat board of bacterial classification, flat board is inverted in 37 ℃ constant temperature culture 24 hours, observe nitrosoguanidine inhibition zone size on every side, choose transparent circle diameter and colony diameter and to the test tube slant, do the production production of hybrid seeds than the bacterium colony culture transferring of (HC)＞1.5;
Starch culture-medium: peptone 10g, sodium chloride 5g, beef extract 5g, soluble starch 2g, distilled water 1000ml, agar 15-20g, 121 ℃ of sterilization 20min;
Second step, the production production of hybrid seeds:
The streptococcus lactis, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, the Lactobacillus casei that filter out are transferred respectively in the eggplant bottle that nutrient agar is housed, under 35 ℃ of temperature, cultivated 45 hours, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white, put into 4 ℃ refrigerator then and preserve;
Nutrient agar: beef extract 3g, peptone 10g, sodium chloride 5g, agar 15-20g, water 1000ml, pH 7.0-7.2,121 ℃ of sterilization 20min.
The liquid at high speed of the 3rd step, bacterial classification is cultivated
(1), takes by weighing each bacterial classification by following percentage by weight: streptococcus lactis 18%, brewer's yeast 20%, bacillus subtilis 13%, Bifidobacterium 23%, Lactobacillus casei 26%;
(2), the bacterial classification liquid at high speed is cultivated: the bacterial classification inoculation that takes by weighing more than inciting somebody to action is in the process culture medium of steam sterilizing, and the ph value transfers to 6.9-7.4; Put into fermentation tank and cultivate, speed of agitator is 200r/min, and cultivation temperature is 35 ℃, throughput 1: 0.6, and fermentation time is 18 hours, obtains composite bacteria agent capable;
Fluid nutrient medium: molasses 15%, peptone 2%, corn steep liquor 5%, sodium chloride 0.5%, yeast extract 0.5%, magnesium phosphate 0.05%, potassium dihydrogen sulfate 0.05%, sterilized water 77.4%;
The 4th step: the solid of composite bacteria agent capable is cultivated synchronously
(1) composite bacteria agent capable is added to insert in the vapor tight tank after solid medium evenly mixes in 15% ratio ferment, 32 ℃ of bottom fermentations 9 days ferment synchronously;
The synchronous culture medium of solid: blue or green chaff 38%, bean cake powder 5%, corn flour 8%, molasses 15%, dusty yeast 0.5%, magnesium phosphate 0.2%, enzyme inducer 0.05%, sterilized water 33.25%, described enzyme inducer are made up of wood sugar and sophorose, and its quality proportioning is: wood sugar 55%, sophorose 45%.
(2) carry out the detection of moisture and total viable count in the sweat, fermentation ends when total viable count 〉=3,000,000,000/g;
(3) product after the fermentation ends is put into boiling drier and is carried out drying, and moisture is controlled in 12%, and dried product is pulverized, and is piglet multienzyme feed addictive finished product.
Embodiment 2
The first step, with embodiment 1
Second the step, with embodiment 1
The liquid at high speed of the 3rd step, bacterial classification is cultivated
(1), takes by weighing each bacterial classification in following ratio: streptococcus lactis 20%, brewer's yeast 23%, bacillus subtilis 16%, Bifidobacterium 26%, Lactobacillus casei 15%;
(2), the bacterial classification liquid at high speed cultivates, with embodiment 1
The 4th step: the solid of composite bacteria agent capable is cultivated synchronously
Composite bacteria agent capable added to insert in the vapor tight tank after carbon source, nitrogenous source, inorganic salts and derivant evenly mix ferment, 32 ℃ of bottom fermentations 8 days ferment synchronously;
The synchronous culture medium of solid is formed and ratio: blue or green chaff 40%, bean cake powder 7%, corn flour 10%, molasses 18%, dusty yeast 0.8%, magnesium phosphate 0.3%, enzyme inducer 0.1%, sterilized water 23.8%; Described enzyme inducer is made up of wood sugar and sophorose, and its quality proportioning is: wood sugar 55%, sophorose 45%;
All the other are with embodiment 1.
In use, add in the pig starter feed by 1-2 ‰ and to feed, when being used for feed granulating, piglet polyzyme additive agent granulation tailored version being added in feed or the premix in the ratio of 1-3 ‰ granulate, this product needs to seal up for safekeeping in that dry ventilation is airtight, should use as early as possible behind the Kaifeng.This product has carried out 3 months test by a definite date in Pudong east plant, and test daily gain has in earlier stage improved 5.87% (P＜0.05), and the daily gain in test later stage has improved 9.12% (P＜0.05), and full phase daily gain improves 7.74%; The feed intake in early stage does not have significant change, but the later stage increased by 3.78%, the price of deed of testing each phase improves 5%-6%.The death rate and control group have relatively reduced by 75% simultaneously.
Claims (3)
1. microorganism polyzyme additive agent that promotes piglet growth and growth, it is characterized in that, form: streptococcus lactis 18-20%, saccharomyces cerevisiae 20-23%, bacillus subtilis 13-16%, Bifidobacterium 23-26%, Lactobacillus casei 15-26% by following percentage by weight raw material;
Preparation method adopts synchronous compound culture method, and all bacterial classifications are cultivated in same culture medium, and it comprises the following steps:
The first step, cultivation bacterial classification
(1) adopting power is that the uviol lamp of 15W adds respectively in the aseptic flat board that fills starch culture-medium each 3ml of bacteria suspension of the streptococcus lactis of preservation, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, Lactobacillus casei at 30cm place, shine and cultivated counting in 1-3 minute, the transparent circle of observation on starch culture-medium chosen transparent circle diameter and colony diameter and cultivated to the test tube slant than the bacterium colony culture transferring of HC＞1.5;
(2) have and place nitrosoguanidine crystallization 0.5g on the 6mm starch culture-medium flat board of bacterial classification, flat board is inverted in 37 ℃ constant temperature culture 24 hours, observe nitrosoguanidine inhibition zone size on every side, choose transparent circle diameter and colony diameter and to the test tube slant, do the production production of hybrid seeds than the bacterium colony culture transferring of HC＞1.5;
Second step, the production production of hybrid seeds:
The streptococcus lactis, saccharomyces cerevisiae, bacillus subtilis, Bifidobacterium, the Lactobacillus casei that filter out are transferred respectively in the eggplant bottle that nutrient agar is housed, under 35-40 ℃ of temperature, cultivated 45-50 hour, treat that eggplant bottle surface lawn is covered with, and can take out when band is yellow in white, put into 2-6 ℃ refrigerator then and preserve;
The 3rd step, bacterial classification liquid at high speed are cultivated
It is middle cultivation that all bacterial classifications that step 2 is cultivated are placed in same fluid nutrient medium by weight percentage, the ph value is to 6.9-7.4, putting into fermentation tank cultivates, speed of agitator is 200r/min-240r/min, cultivation temperature is 35-40 ℃, throughput 1: 0.6, fermentation time is 18-20 hour, obtains composite bacteria agent capable;
When being used for feed granulating, need to add the gelatin of 1% Arabic gum and 1% in the microbial inoculum, wrap processed heat labile Bacillus acidi lactici, Bifidobacterium;
The solid of the 4th step, composite bacteria agent capable is cultivated synchronously
Ferment inserting in the vapor tight tank after even mixing of composite bacteria agent capable 15% ratio adding solid medium, fermentation temperature is 30-35 ℃, fermentation time is 8-10 days, carry out the detection of moisture and total viable count in the sweat, fermentation ends when total viable count 〉=3,000,000,000/g, the product after the fermentation ends, put into boiling drier and carry out drying, moisture is controlled in 12%, and dried product is pulverized, and is piglet multienzyme feed addictive finished product.
2. a kind of microorganism polyzyme additive agent that promotes piglet growth and growth according to claim 1, it is characterized in that, described solid medium is at least two kinds of mixtures in bean cake powder, urea, ammonium sulfate, blue or green chaff, dusty yeast, corn steep liquor, corn flour, molasses, magnesium phosphate, magnesium sulfate, enzyme inducer, the sterilized water, and its weight percent proportioning is: blue or green chaff 38-40%, bean cake powder 5-7%, corn flour 8-10%, molasses 15-18%, dusty yeast 0.5-0.8%, magnesium phosphate 0.2-0.3%, enzyme inducer 0.05-0.1%, sterilized water 23.8-33.25%.
3. a kind of microorganism polyzyme additive agent that promotes piglet growth and growth according to claim 2 is characterized in that described enzyme inducer is made up of wood sugar and sophorose, and its quality proportioning is: wood sugar 50-55%, sophorose 45-50%.
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| CN104472895A (en) * | 2014-10-28 | 2015-04-01 | 亿利资源集团有限公司 | Active microorganism feed for pigs |
| CN104489277A (en) * | 2014-12-31 | 2015-04-08 | 成都枫澜科技有限公司 | Liquid saccharified liquid-containing micro-ecological feed additive and preparation method thereof |
| CN105859839A (en) * | 2016-05-24 | 2016-08-17 | 湖北博大生物股份有限公司 | Biological active peptide for promoting growth of piglet and preparation method and application thereof |
| CN105859839B (en) * | 2016-05-24 | 2019-08-30 | 湖北博大生物股份有限公司 | A kind of biologically active peptide and its preparation method and application promoting piglet growth |
| CN107691844A (en) * | 2017-10-31 | 2018-02-16 | 南京工业大学大丰海洋产业研究院 | A kind of Spartina alterniflora Bifidobacterium nursery-stage pig feed and its compound method |
| CN107889934A (en) * | 2017-11-22 | 2018-04-10 | 李九玲 | A kind of animal health potus for promoting growth |
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