CN106721026A - A kind of nonreactive laying cycle of laying hens compound premixed feed and preparation method and application - Google Patents
A kind of nonreactive laying cycle of laying hens compound premixed feed and preparation method and application Download PDFInfo
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- CN106721026A CN106721026A CN201710006834.7A CN201710006834A CN106721026A CN 106721026 A CN106721026 A CN 106721026A CN 201710006834 A CN201710006834 A CN 201710006834A CN 106721026 A CN106721026 A CN 106721026A
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The invention belongs to field of feed, it is related to the preparation method of a kind of nonreactive laying cycle of laying hens compound premixed feed and the feed and application.Feed relative part composition：5 10 parts of corn protein powder, 8 25 parts of peanut meal, 5 10 parts of rice bran, 15 25 parts of sesame seed meal, 20 50 parts of dietary fiber, 1 10 parts of soybean oil, 0.5% 5 parts of compound premix, 8 15 parts of stone flour, 8 15 parts of calcium monohydrogen phosphate, 2.5 4.5 parts of salt, 13 parts of lysine, 13 parts of threonine, 13 parts of methionine, it is excellent can be precious 0.1 0.5 parts, 13 parts of yeast hydrolyate, 0.3 2 parts of yeast cell wall powder, 0.1 0.5 parts of yeast selenium, 0.1 0.5 parts of zinc methionine, 0.2 0.5 parts of hemoprotein, 0.2 0.5 parts of complex enzyme formulation, 0.8 part of Choline Chloride, 10000 0.2 part of unit phytases.Laying rate, egg production and egg size can be significantly improved.
Description
Technical field
The invention belongs to field of feed, more particularly to a kind of nonreactive laying cycle of laying hens compound premixed feed, and
It is related to the preparation method of the feed and application.
Background technology
With breeding layer chicken popularization, 1% laying hen premix, 3% laying hen premix and 5% laying hen premix are used
Medium-and-large-sized breeding layer chicken increasingly increases, and even up to more than 40%.Cultivation site discovery, uses the chicken farm of premix, egg
Shell poor quality, loose and watery stool, death and culling rate is high, has a strong impact on breeding layer chicken economic benefit, and the annual hidden loss of every laying hen is extremely
It is few more than 5 yuan, account for more than the 30% of laying hen profit.By the pollution such as Escherichia coli, salmonella, respiratory tract during breeding layer chicken
The incidences of disease such as disease, intestines problem, diseases of fallopian tubes are high；Causing the main cause of these problems is, is prepared with premix and helped
Valency material feeds chicken：One is single raw material, only corn, dregs of beans, stone flour and premix, and amino acid balance and calorie-protein balance are bad
Control；Two is big dregs of beans usage amount, and the ANFs of the inside has certain damage to the intestinal health of chicken；Three is crude fiber content
It is not enough, it is difficult to ensure good fiber quality nutrition, it is unfavorable for that metabolic toxicities are discharged；Four is poor mixture homogeneity, and chicken feeding nutrition is uneven
The malnutrition that weighing apparatus brings, produces disease risk.Feed mold easily triggers enteritis during breeding layer chicken；Abuse antiphlogistic
Thing, promotes gut flora unbalance, causes the local superiority of anaerobic bacteria etc. and falls ill, the invasion and attack such as C.perfringens, rotavirus
It is universal etc. the intestines problem for causing.
Poultry gut barrier is thin by normal flora, enteron aisle endocrine and intestines related immune in intestinal mucosa epithelium, enteron aisle
Born of the same parents constitute, and substantial amounts of anaerobic bacteria in intestinal mucosa superficial growth；Protection of intestinal mucosal barrier cells is mainly columnar epithelial cell and a small amount of cup-shaped
The maintenance of cell, endocrine cell and cell intestinal barrier function depends on specific point produced by gut associated lymphoid tissues
Type Immunoglobulin IgA, and nonspecific mechanically and chemically barrier are secreted, such as hydrochloric acid in gastric juice, wriggling, enteric epithelium is closely connected, sticked
Liquid, digestive ferment and normal flora etc..EA plays body health degree and immunologic function with the barrier function of intestinal mucosa
70% effect.Therefore say, it is exactly to support enteron aisle to raise chickens, only intestinal health, and just meeting premunition is strong, and laying hen body just can be healthy,
The inferior health concealed loss that even disease is brought can effectively be reduced.Canadian University of Guelph professor Dr.Julang Li,
Specify that three factors of influence intestinal health：The nutrition of enteron aisle is given, enteron aisle ecological environment, and the disease for eradicating enteron aisle is adjusted
Source；Think " antibacterial peptide ", do not produce drug resistance, effective antibacterial, while contributing to the reparation of tissue.Possess preferable for anti-product
Feature.Dong Ke institutes of China Agricultural University president Guo awards research and thinks to specify that the general character of intestines problem in penetrating judgment, comes from intestines
Road Dysbiosis, epidemic prevention barrier injury, inflammatory reaction, Nutrient Absorption obstacle and stress be metabolized, cause production performance reduction and
Death and culling rate is raised.And zinc is the active component containing Zn enzymes in six big enzymatic reactions, meanwhile, zinc nutrition is close with gut barrier function
Correlation, suitable zinc nutrition is conducive to chicken mucosal immunity, zinc deficiency to be unfavorable for the expression of chicken intestinal mucus albumen；Zinc deficiency is unfavorable for
Small intestine mechanical barrier and its function；The absolute quantity that the main cell B cell cell of humoral immunity is participated in during zinc-deficiency is reduced, and is led
Causing T cell dysplasia, or even zinc-deficiency causes atrophy of thymus gland.
Yeast cell wall powder is rich in immune polysaccharides such as manna oligosacchride (20%), beta glucans (20-40%).Beta glucan is special
Some β -1,3-1,6 helical structures easily recognize by immune system, can improve that animal is non-specific and specific immunity.Sweet dew
Oligosaccharides can improve animal alimentary canal colony balance, prevent enteric pathogenic bacteria to be colonized, with strengthen it is immune, antiviral, anti-oxidant, rush
Various important biological functions such as growth, absorption bacterium and mycotoxin, can be greatly enhanced body immunity.Reducing should
Swash, give full play to the nutritive value of feed, steady production reduces the harm of pathogen, reduces the incidence of disease, reduces death and culling rate, subtracts
Drug cost is cultivated less.Selenium participates in body nospecific immunity, cellular immunity, humoral immunity, in anti-oxidant and immunologic function side
Face plays an important role.Therefore that effect is used in combination with yeast cell wall powder is best for yeast selenium.
Yeast cells hydrolysate contains free amino acid, small peptide, nucleotides and yeast cell wall component, can significantly improve dynamic
Thing feed intake, significantly improves animal intestinal tract health, significantly improves the functions such as immunity of organisms, effective absorbing mycotoxin.
Hydrolysable Tannins acid has following functions：One is convergence anti diar rhea：Its mechanism is when tannic acid is with hydrophobic bond, multiple spot
Hydrogen bond and protein reaction theory.Mainly the peptidyl in its phenolic hydroxyl group and protein main chain, the hydroxyl on side chain, amino and
Carboxyl multiple spot is combined, and promotes moisture and nutriment preferably to absorb.Hydrolysable Tannins acid can effectively reduce livestock and poultry diarrhea rate, carry
The production performance of livestock and poultry high；Two is biocidal property, reduction antibiotic usage；Its mechanism is directly by suppressing microbiological oxidation phosphoric acid
The activity of key enzyme during change, so as to influence the metabolism of the primordial matters such as microorganism.Tannic acid is to Escherichia coli, enteritis
Salmonella, C.perfringens, staphylococcus aureus, campylobacter jejuni are all inhibited.Graz iani etc.,
Molan etc.；Three is antiviral effect.Its mechanism：Tannic acid is easily combined with protein, into viral internal and prevent glycoprotein answer
System, can solidify the plasm in organism, and the activity with suppression viral internal enzyme is so that virus apoptosis.Dietary fiber master
The trophism is wanted to be：Develop in stimulating gastrointestinal road；Stomach normal creepage of gastrointestinal functions is maintained, loose stool is reduced and constipation is occurred；
Searching for food appropriate crude fibre can be by beneficial bacterium fermentative degradation in rear intestinal, pre- anti-diarrhea, simultaneously as coarse-fibred suction
It is aqueous, the moisture absorption in excrement can be reduced loose stool problem；Maintain intestinal microbial balance；Detoxication：Dietary fiber
Some harmful substances produced in adsorbable feed and alimentary canal, excrete it.Appropriate dietary fiber in rear intestinal fermentation,
The pH of intestinal contents after can reducing, suppresses the growth of the pathogens such as Escherichia coli, prevents diarrhoea.
The invention of application number CN201110355692.8《A kind of environmental protection, nonreactive type broiler chicken later stage mixed feed》, describe
Its animal digestion utilization rate is high, effectively maintains archenteric flora balance, low cost to reduce environmental pollution.The present invention is by following original
Material is made：Corn 580-630 weight portions, dregs of beans 90-120 weight portions, wheat-middlings 85-105 weight portions, rapeseed meal 25-45 weight portions,
DDGS50-70 weight portions, corn protein powder 20-40 weight portions, meat meal tankage 20-30 weight portions, calcium carbonate 3-7 weight portions are combined
Vitamin 0.3-0.7 weight portions, grease 10-30 weight portions, lysine 2.0-6.0 weight portions, methionine 0.2-0.6 weight portions,
Threonine 0.1-0.5 weight portions, salt 2.0-3.0 weight portions, antioxidant 0.3-0.7 weight portions, probiotics 0.3-0.7
Weight portion and phytase 0.1-0.5 weight portions.The invention is directed to the product of broiler chicken, and one is only have selected in terms of substitute antibiotics
Probiotics is planted, useful effect is not reached in practice, the method for current substitute antibiotics must use system synthesis measure.
Publication number:The invention of 102742738A《A kind of laying cycle of laying hens premixed feed for improving eggshell quality》Disclose
A kind of laying cycle of laying hens premixed feed for improving eggshell quality, is made up of the component of following part of note by weight：0-40 parts of dregs of beans,
8-11 parts of di-calcium phosphate, 0-15 parts of enzyme power peptide, 0-20 parts of meat meal tankage, 10-14 parts of medical stone, 5 parts of salt, 2 parts of sodium bicarbonate, 2
Part choline, 10-21 parts of stone flour, 0.3-0.6 parts of 98% glycine betaine, 1.6-2.2 parts of methionine, 0.24-0.3 parts of phytase, 0.1-
0.2 part of antioxidant, 0.06 part of producing enzyme probiotic, 4 parts of many ore deposits of laying hen, 0.6 part of laying hen multidimensional, 0-23 parts of zeolite powders.The present invention
Scientific and reasonable collocation, raw material preferred compositions meet the nutritional need of laying hen, are combined using advanced additive, improve premunition,
Enhancing anti-stress ability, the use of high-quality complex enzyme formulation improves laying hen stomach and intestine biological structure, the harmful substance such as nitrogen phosphorus in chicken manure
Discharge is reduced, and the harmful gas concentration such as ammonia declines in hen house, can improve efficiency of feed utilization, and laying rate improves average extension chicken and produces
The egg phase, feeding cost is reduced, reduce the laying hen death rate, eggshell color can be effectively improved, strengthen eggshell strength, reduce egg damaged
Rate.The invention core point is to improve eggshell quality, does not account for improving premunition and substitute antibiotics problem, and the present invention is main
For improving laying hen immunologic function and improving premunition, medication in breeding layer chicken is reduced.
Publication number:The invention of 106234822A《Laying cycle of laying hens feed and processing method》Disclosed laying cycle of laying hens is raised
Material, is prepared from the following ingredients in parts by weight：30-60 parts of plant stem-leaf powder, cereal and/or beans powder 150-250
Part, 50-150 parts of plant shell class powder, Chinese medical extract 7-15 parts, salt 1-3 parts, 4-7 parts, fish meal 1-3 parts of function powder.It is described
Function powder is mixed for any one or a few in amino acid powder, vitamin powder, mineral matter powder, stone flour.Wherein axis
It is native state although leaf powder, vegetable shell powders content of cellulose are high, unfavorable laying hen absorbs, can hinders on the contrary
The absorption of nutriment, using Chinese medical extract, although avoid the harm of antibiotic, but it is slow to work, and consumption requirement compared with
Greatly.
Publication number:The invention of 105995177A《A kind of poultry egg laying phase feed and preparation method thereof》Disclosed feed by with
The raw material composition of lower weight portion：Corn flour 30-40 parts, soya-bean cake 5-10 parts, broad bean skin 5-10 parts, watermelon peel 3-5 parts, earthworm powder 5-
8 parts, beggar-ticks 2-3 parts, 15-20 parts of mountain lettuce, fish meal 5-10 parts, dandelion 2-5 parts, methionine 0.1-0.5 parts, oyster shell whiting 2-
3 parts, pine needle meal 5-10 parts, salt 2-5 parts.The present invention sets about from the biological nature of poultry, feed ingredient is improved, nutrition
Equilibrium, good palatability is easily digested and assimilated by poultry, each composition collective effect, improves the laying rate of poultry, is improved poultry and is produced
The interior quality of egg；Promote the fast-growth of poultry, reduce feedstuff-meat ratio；The immunity of poultry is improved, strengthens the disease-resistant energy of poultry
Power.But many raw materials of the invention are not conventional raw materials, raw material is not easy to obtain and is subject to seasonal restrictions larger, and being unfavorable for promoting should
With.
Publication number:The invention of 105876116A《The feed formula of the laying cycle of laying hens of fish meal is substituted using worm for fishing》Disclose
A kind of utilization worm for fishing substitutes the laying cycle of laying hens feed formula of fish meal, and the formula is made up of the weight proportion of following raw material：It is beautiful
Rice 68%, worm for fishing 30%, calcium monohydrogen phosphate 1.7%, salt 0.3%.Due to the fish meal wretched insufficiency in tradition cultivation formula, it is difficult
Digestion, the worm for fishing in the formula is inexhaustible, and digestion is easy to again, therefore, the formula replaces tradition with worm for fishing
Fish meal in cultivation formula, in laying cycle of laying hens, advantageously reduces cost, makes yolk red, and egg white is thick, extends ovipository cycle, drop
Low actual.The formula is applied suitable for the laying hen of laying rate 5%- peak phases.The core point of the invention is making for worm for fishing
With significantly different in core point of the invention.
Publication number:The invention of 105851613A《A kind of feeding feed stuff of laying hen》A kind of feeding feed stuff of laying hen is disclosed,
Including chicken feed, young chicken feed, laying period feed, wherein the laying period feed includes corn 65-70 according to mass parts
Part, wheat bran 10-15 parts, dregs of beans 10-20 parts, rapeseed dregs 5-10 parts, fish meal 3-5 parts, stone flour 3-5 parts, bone meal 1-3 parts, salt 1-2
Part, amino acid 0.5-1 parts, vitamin 1-2 parts, 1-2 parts of trace element.The feeding feed stuff of laying hen of the present invention, in the difference of laying hen
In developmental process, there is provided different feed formulas, the feed not only has abundant nutritional ingredient, and low cost, raiser
Feed ingredient proportioning can neatly be changed as needed.The invention component is excessively simple, belongs to conventional feed, and raising disappears
The bioactivator of rate such as enzyme preparation, probiotics etc. are not all used, and standardization level is low, it is impossible to meet laying hen product
, easily there is laying hen and body deposit employed to lay eggs in egg phase nutritional need, laid eggs demand as cost meets maximum with health, cause body
Weight is not up to standard, the phenomenon of premunition reduction.
In sum, the laying hen premix for commonly using at present has that nutrition is unbalanced, easily causes and uses premix
Chicken farm, eggshell quality is bad, and loose and watery stool, death and culling rate is high, and cultivation dosage is big etc. to have a strong impact on breeding layer chicken economic benefit etc.
Problems.
The content of the invention
The present invention from dietary fiber nutrition, the additive of intestinal health, is improved according to laying hen physiological function and nutritional characteristic
The aspects such as immunologic function nutrition, functional amino are set about, and effectively reduce breathing problem, intestines problem, diseases of fallopian tubes etc.,
Eggshell quality and intestinal health level are improved, and then reduces the incidence of disease, reduce cultivation medication, there is provided safe scrambled egg product, improved
Breeding layer chicken economic benefit.
To reach above-mentioned purpose, the present invention uses following technical scheme：
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
Corn protein powder 5-10 parts, peanut meal 8-25 parts, rice bran 5-10 parts, sesame seed meal 15-25 parts, dietary fiber 20-50
Part, soybean oil 1-10 parts, 0.5% 5 parts of compound premix, calcium monohydrogen phosphate 8-15 parts, salt 2.5-4.5 parts, relies by stone flour 8-15 parts
Propylhomoserin 1-3 parts, threonine 1-3 parts, methionine 1-3 parts, it is excellent can 0.1-0.5 parts of treasured, yeast hydrolyate 1-3 parts, yeast cell wall
Powder 0.3-2 parts, yeast selenium 0.1-0.5 parts, zinc methionine 0.1-0.5 parts, hemoprotein 0.2-0.5 parts, complex enzyme formulation
0.2-0.5 parts, 0.8 part of Choline Chloride, 10,000 0.2 part of unit phytases；
Further, the complex enzyme formulation is made up of zytase, cellulase, lipase and protease；
Further, the dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasound
Extract, biological enzymolysis after fermentation is obtained；
The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo
Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction
20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme
70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25-30 DEG C, adds the glucose of mixture quality 3-5%, 0.2-
0.5% mixed bacteria, 25-30 DEG C is cultivated 48-72 hours, and speed of agitator is 20-30r/min, and culture was added by 50-55 hours
The Cys of mixture quality 0.005-0.1% and the Radix Glycyrrhizae Ultramicro-powder of 0.05-0.1%, be concentrated under reduced pressure after fermentation ends,
Freeze-drying, low-temperature grinding are to particle diameter for 0.1-0.3mm obtains final product dietary fiber；
The mixed bacteria contains following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=
100000000000/g, saccharomycete >=10,000,000,000/g, lactic acid bacteria >=5,000,000,000/g；
Preferably, the saccharomycete is saccharomyces cerevisiae；
Further, the saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae)
Tlj2016, deposit number is CGMCC No.12789；
The biology enzyme is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 is uniform
Mixing；
Further, the onion powder, preparation method thereof comprises the following steps：
Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and onion weight 0.03% is added afterwards
Mixing enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid in 55 DEG C, 300W, 80KHz bar
Ultrasonic extraction 20min under part, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；
The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, wood
Dextranase 0.5；
The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum
0.05MPa, 45-55 DEG C of triple effect, vacuum 0.04MPa.
Further, the preparation method of the yeast hydrolyate, comprises the following steps：
(1) Shaking culture
The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of trainings
Foster 30h obtains yeast starter liquid；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30
DEG C, throughput 6L/min, tank pressure 0.03MPa, 500rpm carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, once
Property addition final concentration of 25mmol/L Cys, total fermentation time is 50h, obtains zymotic fluid；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；
(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio
Slurry, adjusts pH value 6-8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C of -50 DEG C of insulation self-dissolving 24h；The mixed enzyme system of addition
Agent, consumption is the 1%-1.5% of yeast paste quality；Stirring, enzymatic hydrolysis 10h-24h.
The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase equivalent
Mix；
(5) being spray-dried 120 DEG C of -140 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.
Preferably, the yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, protect
It is CGMCC No.12789 to hide numbering.
Present invention simultaneously provides a kind of preparation method of nonreactive laying cycle of laying hens compound premixed feed, including following step
Suddenly：
According to formula accurately weigh each component raw material, by complex enzyme formulation, yeast cell wall powder, yeast hydrolyate and
0.5% compound premix is pre-mixed, then arrogant to small by weight to sequentially add various raw materials, is well mixed, processing
Granulation, obtains final product nonreactive laying cycle of laying hens compound premixing and feed.
The application process of nonreactive laying cycle of laying hens compound premixed feed is：According to following proportioning, corn 62%, dregs of beans
20%, stone flour 8%, nonreactive laying cycle of laying hens compound premixed feed 10%；After corn, dregs of beans, stone flour are crushed respectively, with this
Invention compound premixed feed is used after mixing.
Beneficial effect
The present invention from dietary fiber nutrition, the additive of intestinal health, is improved according to laying hen physiological function and nutritional characteristic
The aspects such as immunologic function nutrition, functional amino are set about, and effectively reduce breathing problem, intestines problem, diseases of fallopian tubes etc.,
Eggshell quality and intestinal health level are improved, and then reduces the incidence of disease, reduce death and culling rate, reduce antibiotic usage in cultivation, carried
For safe scrambled egg product, breeding layer chicken economic benefit is improved.Show through feeding experiment：Laying rate improves 3.37%, and egg production is carried
High by 7.65%, egg size improves 4.13%, and the death rate is made money 5.39 yuan every chicken more than control group reduction by 3.1%, total antioxidation energy
The Antioxidant Indexes such as power T-AOC improve 54.1%, and effect is obvious.
Especially with dietary fiber prepared by ad hoc approach, soluble dietary fibre content is high, bioactivity is strong, and protects
A number of Water insoluble dietary fiber is stayed, has been coordinated with prebiotics, gut flora can have been significantly improved, improved enteron aisle benefit
The resident time of the quantity of raw flora, regulation and maintenance beneficial bacteria of intestinal tract group, strengthens digestion and the absorbability of enteron aisle, improves dynamic
Thing immunity.The preparation of dietary fiber, enzymolysis, ultrasonic extraction is combined with superfine communication technique, using specific technique bar
Part, while soluble dietary fibre content is improved, and can improve soluble dietary fiber and Water insoluble dietary fiber
Characteristic, its retentiveness, dilatancy, thickening property have raising in various degree.Dietary fiber, onion are prepared from onion powder：Nature and flavor
Pungent-warm, sweet delicate, in addition to containing common nutriment, prostaglandin A and sulphur Amino acid contained by it have expansion of blood vessels,
Regulation blood fat, prevents the effect of artery sclerosis, assigns dietary fiber of the present invention the key property different from similar dietary fiber.
Prepared by yeast hydrolysate is prepared using special process, the content of glutathione can be significantly improved, glutathione has anti-
The functions such as oxidation, removing free radical, removing toxic substances, strengthen immunity, anti-aging, anticancer, the harm of anti-radiation line, are important functions
The factor, can improve the skin health of chick, and feather glossiness, the work with enhancing immunologic function, enriched nutritive
With.Acted synergistically with prebiotics etc., the propagation of beneficial bacterium can be promoted, improve the development of chick intestinal villi, reduce diarrhea rate, carried
Animal immunizing power high；The preservation of bacteria strain tlj2016 of high yield glutathione is particularly added, glutathione can be more significantly improved and be contained
Amount, after fermentation ends, the content of GSH can reach 3308mg/L in zymotic fluid, and glutathione is used as important anti-oxidant in vivo
Agent and free radical scavenger, are such as combined with free radical, heavy metal, the poisonous substance being harmful in body can be converted into harmless thing
Matter, excretes external, the physical function to improving incubation laying hen, very helpful.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change
Belong to protection scope of the present invention.
The Wine brewing yeast strain is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterium
Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on July 15th, 2016
It is CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, postcode 100101.
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；
The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following
Step is obtained：
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing glutathione GSH abilities) → passage stabilization is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask
Property experiment.
By the bacterial strain tlj2016 obtained after mutagenesis, its glucose tolerance and Cys tolerance are equal
It is improved, cell density, another aspect Cys tolerance energy on the one hand can be improved under high concentration glucose culture
The raising of power is beneficial to GSH and largely synthesizes in intracellular, so as to improve the ability that bacterial strain mass produces GSH.
Saccharomyces cerevisiae of the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration grape
GSH is produced under the conditions of sugar；3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；Tolerate the energy of Cys
Power is far above starting strain, slow growth is remained under the effect of 5mmol/L Cys, in 40mmol/LL- cysteines
Remain to keep GSH largely to synthesize under effect；Salt resistance ability reaches 18%, is conducive to extending its application field.
1.DES mutagenic and breedings
1) ring of starting strain 1 one on test tube slant is taken on super-clean bench, is accessed equipped with 50mL malt extract mediums
In 250mL triangular flasks, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions are taken, 5000rpm centrifugation 10min collects thallines, with brine 2 times.
3) 10 are diluted to pH7.0 phosphate buffers7Individual/mL bacteria suspensions.
4) kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspensions, 0.4mL DES are taken and is being placed in advance in the 150mL of rotor
It is sufficiently mixed in triangular flask, makes DES ultimate densities be 1% (v/v).
5) the 150rpm reactions 30min in 30 DEG C of shaking tables, takes 1mL mixed liquors, adds 0.5mL 25%Na2S2O3In solution
Only react.
6) dilution spread is in the malt extract medium plate containing 150g/L KCl, the picking bacterium after 30 DEG C of cultures 2-3 days
The bacterial strain 1 of the maximum that falls.
2. nitrosoguanidine mutagenesis
1) ring of saccharomyces cerevisiae bacteria strain 1 one on test tube slant is taken on super-clean bench, is accessed and 50mL brewer's wort cultures is housed
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.
2) 5mL bacterium solutions 5000rpm centrifugation 10min collects thallines are taken, with brine 2 times.
3) 10 are diluted to pH6.0 phosphate buffers7Individual/mL bacteria suspensions.
4) 10mL bacteria suspensions are taken to be transferred in 100mL triangular flasks, the NTG of 10mg is added, final concentration of 10mg/mL is configured to
NTG solution, and add 4-5 drop acetone, be beneficial to NTG dissolvings.
5) the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm are centrifuged 10min collects thallines, use SPSS
Wash for several times, stopped reaction.
6) appropriate dilution spread, takes the bacterium solution 0.2mL of last dilution factor, coats the brewer's wort culture containing 200g/L KCl
In base plate.Picking colony 20 after being cultivated 2-3 days at 30 DEG C.
3. shaking flask primary dcreening operation
1) take above-mentioned each ring of 20 S. cervisiaes respectively on super-clean bench, be respectively connected to equipped with 50mL brewer's wort cultures
In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 12h or so, thalline is in mid log phase.
2) 5mL bacterium solutions are taken, is accessed equipped with the 250mL in the hypertonic malt extract mediums of 50mL (concentration of glucose is 300g/L)
In triangular flask, 200rpm, 30 DEG C are cultivated 3-4 days, daily detection concentration of glucose and concentration of alcohol change.After fermentation ends, than
Compared with 20 plants of glucose and ethanol wear rate of strain, final remaining sugar concentration and concentration of alcohol, glucose to the conversion ratio of ethanol
And heteroacid content.
3) glucose consumption rate is fast, final remaining sugar concentration is low and concentration of alcohol is high 5 plants of bacterium are named as Y-1, Y- for selection
2, Y-3, Y-4, Y-5.
4. ferment secondary screening
By 5 plants of bacterium Y-1, Y-2, Y-3, Y-4, Y-5 and starting strain of gained in step 3, respectively according to 10% inoculation
Amount, the 150rpm in the 250mL shaking flasks equipped with 30mL fluid nutrient mediums, 30 DEG C of culture 30h, takes zymotic fluid and determines GSH concentration；
Fluid nutrient medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1；
GSH assay methods：Fresh yeast, at 30 DEG C, 40% Ethanol Treatment 3h, in centrifuging and taking are obtained after zymotic fluid centrifuge washing
Clear liquid is cooked GSH determination samples；
GSH measure is carried out using alloxan method：Its principle is that-the SH on GSH reacts with alloxan, the material of generation
There is absworption peak at 305nm, and it is linear with glutathione concentrations, therefore can be quantitative determined with ultraviolet specrophotometer
GSH contents.
Table 1：GSH testing results
| Bacterial strain | Starting strain | Y-1 | Y-2 | Y-3 | Y-4 | Y-5 |
| GSH concentration (mg/L) | 127.9 | 195.7 | 172.3 | 263.2 | 216.5 | 185.2 |
As seen from the results in Table 1, bacterial strain Y-3 has highest GSH fermentabilities, it is thus determined that Y-3 is final production bacterium
Strain, and it is named as tlj2016.
5. genetic stability experiment
Continuous ten passages on inclined-plane by tlj2016 bacterium, and detect the hair after passage every time with the method for shaking flask secondary screening
Ferment situation.Experiment finds that continuous ten passages on inclined-plane, the strain proterties does not have significant change, and property indices are all just
Often, illustrate that the genetic stability of the strain is stronger.
Fermented under the conditions of saccharomyces cerevisiae tlj2016 sugar high and produce GSH capacity experimentals
(1) Shaking culture
The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
By seed liquor by the inoculum concentration of 10% mass percent, access and be equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C,
Throughput 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably
The Cys of final concentration of 25mmol/L are added, total fermentation time is 50h；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.
Saccharomyces cerevisiae tlj2016L- cysteines tolerance is tested
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
Table 2：Starting strain Cys tolerance
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Starting strain dry weight g/L | 22.6 | 15.7 | 10.2 | 4.3 | 2.2 | 0.8 |
| GSH concentration (mg/L) | 35.6 | 46.7 | 43.2 | 40.7 | 37.9 | 25.3 |
Table 3：Tlj2016L- cysteine tolerances
| Cys concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
| GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing,
And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；From the results shown in Table 3,
Under low concentration Cys, tlj2016 still is able to slow growth, with the raising of Cys concentration, tlj2016 bacterial strains
Dry cell weight slowly decline, and GSH concentration sustainable growths, before this result is beneficial in GSH production processes by addition
Body amino acid-Cys promote the production of GSH.
Saccharomyces cerevisiae tlj2016 salt resistance abilities are tested
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%,
10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3
Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions solid in YPD
It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat
Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring
Survived in border, still there is vigor under high salt conditions, can be applied to consume sugar in the high salt food processing process such as soy sauce, curing food
Produce glutathione.
Table 4：Salt resistance ability detection (× 107cfu/ml)
Embodiment 1
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
8 parts of corn protein powder, 19 parts of peanut meal, 6 parts of rice bran, 20 parts of sesame seed meal, 20 parts of dietary fiber, 1.5 parts of soybean oil,
0.5% 5 parts of compound premix, 10 parts of stone flour, 12 parts of calcium monohydrogen phosphate, 3.5 parts of salt, 1.3 parts of lysine, 1 part of threonine, egg ammonia
1.5 parts of acid, it is excellent can be precious 0.2 part, 1 part of yeast hydrolyate, 0.5 part of zinc methionine, 0.5 part of complex enzyme formulation, Choline Chloride 0.8
Part, 10,000 0.2 part of unit phytases, 1 part of yeast cell wall powder, 0.3 part of yeast selenium, 0.3 part of hemoprotein；
The dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasonic extraction, life
Thing enzymolysis after fermentation is obtained；
The dietary fiber preparation method is comprised the following steps：
By onion powder, barley fiber, common oats fibre, wheat embryo fiber in mass ratio 1:3:8:3 uniform mixing, add mixed
The water that 8 times of compound quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min is 5.5 with newborn acid for adjusting pH value, adds mixing
The biology enzyme of amount of substance 0.2%, 45min is digested in 50 DEG C, and go out enzyme；70 DEG C of enzymolysis liquid, 18-20MPa homogeneous, material is cooled to 28
After DEG C, the glucose of addition mixture quality 4%, 0.4% mixed bacteria, 28 DEG C are cultivated 60 hours, and speed of agitator is 25r/
Min, culture to 52 hours Cys and 0.1% Radix Glycyrrhizae Ultramicro-powder of addition mixture quality 0.05%, fermentation ends
Afterwards be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.2mm obtains final product dietary fiber；
The mixed bacteria contains following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=
100000000000/g, saccharomycete >=10,000,000,000/g, lactic acid bacteria >=5,000,000,000/g；
The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
The biology enzyme is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 is uniform
Mixing；
The onion powder, preparation method thereof comprises the following steps：
Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and onion weight 0.03% is added afterwards
Mixing enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid in 55 DEG C, 300W, 80KHz bar
Ultrasonic extraction 20min under part, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；
The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, wood
Dextranase 0.5；
The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum
0.05MPa, 45-55 DEG C of triple effect, vacuum 0.04MPa.
The preparation method of the yeast hydrolyate, comprises the following steps：
(1) Shaking culture
The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of trainings
Foster 30h obtains yeast starter liquid；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30
DEG C, throughput 6L/min, tank pressure 0.03MPa, 500rpm carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, once
Property addition final concentration of 25mmol/L Cys, total fermentation time is 50h, obtains zymotic fluid；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；
(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio
Slurry, adjusts pH value 7, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 45 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption
It is the 1.2% of yeast paste quality；Stirring, enzymatic hydrolysis 18h.
The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase equivalent
Mix；
(5) being spray-dried 130 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789.
A kind of preparation method of nonreactive laying cycle of laying hens compound premixed feed, comprises the following steps：
According to formula accurately weigh each component raw material, by complex enzyme formulation, yeast cell wall powder, yeast hydrolyate and
0.5% compound premix is pre-mixed, then arrogant to small by weight to sequentially add various raw materials, is well mixed, processing
Granulation, obtains final product nonreactive laying cycle of laying hens compound premixing and feed.
The application process of the nonreactive laying cycle of laying hens compound premixed feed is：According to following proportioning, corn 62%, beans
The dregs of rice 20%, stone flour 8%, nonreactive laying cycle of laying hens compound premixed feed 10% of the present invention；By corn, dregs of beans, stone flour difference powder
After broken, used after being mixed with compound premixed feed of the present invention.
Embodiment 2
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
10 parts of corn protein powder, 18 parts of peanut meal, 5 parts of rice bran, 28 parts of dietary fiber, 1.5 parts of soybean oil, 0.5% is combined
5 parts of premix, 10 parts of stone flour, 12 parts of calcium monohydrogen phosphate, 3.5 parts of salt, 1.3 parts of lysine, 1 part of threonine, 1.5 parts of methionine,
0.2 part of hemoprotein, 0.3 part of yeast selenium, 0.5 part of yeast cell wall powder, 0.5 part of complex enzyme formulation, 0.8 part of Choline Chloride, 1
Ten thousand 0.2 part of unit phytases, 15 parts of sesame seed meal, it is excellent can be precious 0.5 part, 3 parts of yeast hydrolyate, 0.1 part of zinc methionine；
The dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasonic extraction, life
Thing enzymolysis after fermentation is obtained；
The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo
Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction
20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme
70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25 DEG C, adds the glucose of mixture quality 5%, 0.5% mixing
Strain, 25 DEG C are cultivated 72 hours, and speed of agitator is 30r/min, culture to 50 hours L- of addition mixture quality 0.005% half
Cystine and 0.1% Radix Glycyrrhizae Ultramicro-powder, after fermentation ends be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.1mm is
Obtain dietary fiber；
The mixed bacteria contains following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=
100000000000/g, saccharomycete >=10,000,000,000/g, lactic acid bacteria >=5,000,000,000/g；
The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
The biology enzyme is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 is uniform
Mixing；
The onion powder, preparation method thereof comprises the following steps：
Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and onion weight 0.03% is added afterwards
Mixing enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid in 55 DEG C, 300W, 80KHz bar
Ultrasonic extraction 20min under part, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；
The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, wood
Dextranase 0.5；
The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum
0.05MPa, 45-55 DEG C of triple effect, vacuum 0.04MPa.
The preparation method of the yeast hydrolyate, comprises the following steps：
(1) Shaking culture
The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of trainings
Foster 30h obtains yeast starter liquid；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30
DEG C, throughput 6L/min, tank pressure 0.03MPa, 500rpm carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, once
Property addition final concentration of 25mmol/L Cys, total fermentation time is 50h, obtains zymotic fluid；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；
(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio
Slurry, adjusts pH value 6, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption
It is the 1.5% of yeast paste quality；Stirring, enzymatic hydrolysis 10h.
The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase equivalent
Mix；
(5) being spray-dried 120 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789.
A kind of preparation method and application method of nonreactive laying cycle of laying hens compound premixed feed, with embodiment 1；
Embodiment 3
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
5 parts of corn protein powder, 8 parts of peanut meal, 36 parts of dietary fiber, 1.5 parts of soybean oil, 0.5% 5 parts of compound premix,
10 parts of stone flour, 12 parts of calcium monohydrogen phosphate, 3.5 parts of salt, 1.5 parts of lysine, 1 part of threonine, 1.5 parts of methionine, it is excellent can precious 0.2
Part, 0.3 part of yeast selenium, 0.5 part of yeast cell wall powder, 0.5 part of complex enzyme formulation, 0.8 part of Choline Chloride, 10,000 unit phytases
0.2 part, 5 parts of rice bran, 25 parts of sesame seed meal, 3 parts of yeast hydrolyate, 0.5 part of zinc methionine, 0.5 part of hemoprotein；
The dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasonic extraction, life
Thing enzymolysis after fermentation is obtained；
The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo
Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction
20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme
70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 30 DEG C, adds the glucose of mixture quality 3%, 0.2% mixing
Strain, 30 DEG C are cultivated 48 hours, and speed of agitator is 20r/min, culture to 50 hours Guangs of L- half of addition mixture quality 0.1%
Propylhomoserin and 0.05% Radix Glycyrrhizae Ultramicro-powder, after fermentation ends be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.3mm is
Obtain dietary fiber；
The mixed bacteria contains following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=
100000000000/g, saccharomycete >=10,000,000,000/g, lactic acid bacteria >=5,000,000,000/g；
The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled
Number be CGMCC No.12789；
The biology enzyme is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 is uniform
Mixing；
The onion powder, preparation method thereof comprises the following steps：
Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and onion weight 0.03% is added afterwards
Mixing enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid in 55 DEG C, 300W, 80KHz bar
Ultrasonic extraction 20min under part, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；
The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, wood
Dextranase 0.5；
The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum
0.05MPa, 45-55 DEG C of triple effect, vacuum 0.04MPa.
The preparation method of the yeast hydrolyate, comprises the following steps：
(1) Shaking culture
The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of trainings
Foster 30h obtains yeast starter liquid；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30
DEG C, throughput 6L/min, tank pressure 0.03MPa, 500rpm carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, once
Property addition final concentration of 25mmol/L Cys, total fermentation time is 50h, obtains zymotic fluid；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0。
(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；
(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio
Slurry, adjusts pH value 8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 50 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption
It is the 1% of yeast paste quality；Stirring, enzymatic hydrolysis 24h.
The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase equivalent
Mix；
(5) being spray-dried 140 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.
The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number
It is CGMCC No.12789.
A kind of preparation method and application method of nonreactive laying cycle of laying hens compound premixed feed, with embodiment 1.
Embodiment 4
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
6 parts of corn protein powder, 25 parts of peanut meal, 10 parts of rice bran, 15 parts of sesame seed meal, 50 parts of dietary fiber, 1 part of soybean oil,
5 parts of compound premix, 8 parts of stone flour, 15 parts of calcium monohydrogen phosphate, 2.5 parts of salt, 3 parts of lysine, 3 parts of threonine, 1 part of methionine is excellent
Can be precious 0.1 part, 1 part of yeast hydrolyate, 0.3 part of yeast cell wall powder, 0.5 part of yeast selenium, 0.1 part of zinc methionine, ferroheme egg
White 0.2 part, 0.2 part of complex enzyme formulation, 0.8 part of Choline Chloride, 10,000 0.2 part of unit phytases；
The dietary fiber preparation method is with embodiment 1；
The onion powder, preparation method thereof is with embodiment 1；
The preparation method of the yeast hydrolyate is with embodiment 1；
A kind of preparation method and application method of nonreactive laying cycle of laying hens compound premixed feed, with embodiment 1；
Embodiment 5
A kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
8 parts of corn protein powder, 13 parts of peanut meal, 8 parts of rice bran, 20 parts of sesame seed meal, 20 parts of dietary fiber, 10 parts of soybean oil,
5 parts of compound premix, 15 parts of stone flour, 8 parts of calcium monohydrogen phosphate, 4.5 parts of salt, 1 part of lysine, 1 part of threonine, 3 parts of methionine is excellent
Can be precious 0.5 part, 1 part of yeast hydrolyate, 2 parts of yeast cell wall powder, 0.1 part of yeast selenium, 0.5 part of zinc methionine, hemoprotein
0.4 part, 0.4 part of complex enzyme formulation, 0.8 part of Choline Chloride, 10,000 0.2 part of unit phytases；
The dietary fiber preparation method is with embodiment 2；
The saccharomycete is the conventional saccharomyces cerevisiae in this area；
The onion powder, preparation method thereof is with embodiment 2；
The preparation method of the yeast hydrolyate is with embodiment 2；
The saccharomycete is the conventional saccharomyces cerevisiae in this area；
A kind of preparation method and application method of nonreactive laying cycle of laying hens compound premixed feed, with embodiment 1；
In above-described embodiment 1-5
0.5% compound premix, Liaoning Hefeng Animal Husbandry Co., Ltd's production, Q/HF J02.03-2012, the Liao Dynasty raises pre-
Word (2013) 003024；
10000 unit phytases：The new ocean development in science and technology Co., Ltd in Beijing, production licence number adds (2012) for feeding
0017, authentication code：Raise (adding) word (2012) 403711 in capital；
Complex enzyme formulation：Shenyang Fengmei Biotechnology Co., Ltd. produces, and production licence number adds (2011) 1986 for feeding,
Authentication code：(adding) word (2011) 040008 is raised by the Liao Dynasty；
It is excellent can be precious：Italian Shi Hua companies, (2011) raise outward quasi- word 328；Product active ingredient：Hydrolysable Tannins acid
75%；Main Function：Convergence control diarrhoea, antibacterial, antiviral, the natural anti-oxidation of selectivity；Improve eggshell color, reduce dead
Rate, improves chicken coop air quality.
Test example：
Below by way of the contrast test of feed similar with this area, nonreactive laying cycle of laying hens compound premixing of the present invention is closed and is raised
Expect that the influence to laying hen immunologic function and egg laying performance is described further
1 experiment material and method
1.1 test periods：Test in August in 2015 20 days --- on December 20th, 2015, altogether 121 days, wherein prerun
7 days phases.
1.2 test sites：Experiment is implemented in Huan Ren Xinhua chicken house field experiment.
1.3 test groups are designed：Test group is divided into control group and feed group of the present invention；According to formula as below：Corn 62%, beans
The dregs of rice 20%, stone flour 8%, compound premixed feed 10%, after respectively crushing corn, dregs of beans, stone flour, with various embodiments of the present invention
The premix of preparation, the preferably similar premix of market sale are mixed, and test group feeds that to be mixed with embodiment of the present invention 1-5 pre- respectively
The feed of batch mixing, control group fed is mixed with the feed of commercially available similar premix.Experiment is using single factor experiment design, random choosing
1152 blue brown 220 age in days laying hens in sea are selected, is divided into 6 groups, every group sets 6 repetitions, and each repeats 32 chickens.
Corn unit weight requirement more than 710, dregs of beans crude protein more than 44%, penicillium toxin reaches 65% for more than 4.0 millimeters.
1.4 feeding methods：Manual feeding, three times a day, free choice feeding；Free water, running water, 15-21 DEG C of water temperature.
1.5 hen house temperatures：Using longitudinal ventilation, hen house temperature is controlled at 20-22 DEG C.It is strict according to the blue brown feeding and management in sea
Control.
1.6 testing inspection indexs：1) weigh：Original body mass, terminates body weight.Each repeats to weigh 1 cage chicken, by only weighing.
Feed intake：Free choice feeding, by week clearance feed consumption rate；Period accumulative death chicken number.
2) lay eggs a piece number, egg size, broken egg and soft-preserved egg；3) haematogenic immunity index：IgA,IgG,IgM；
Antioxidant Indexes：Glutathione peroxidase GSH-PX, hepatocuprein T-SOD, MDA MDA and total
Oxidation resistance T-AOC.Blood-sampling method uses Culling heart blood.
1.7 data process＆analysis
All experimental datas are arranged using excel forms, then carry out statistical analysis with SPSS17.0 softwares, as a result
Represented using average ± standard deviation (X ± SD).
2 result of the tests
By 6 hen houses, 6 batch experiment Indexs measure result statistics, each group of data of the present invention is averaged, statistics knot
Fruit such as table 5, table 6 and table 7：
Table 5：Influence of the present invention to performance in layers
| Original body mass (kilogram) | Terminate body weight (kilogram) | Body weight gains (gram) | The death rate (%) | |
| Of the present invention group | 1.85 | 1.96 | 110 | 0.8 |
| Control group | 1.86 | 1.89 | 30 | 3.9 |
From table 5, of the present invention group during 220 ages in days to 341 ages in days body weight gains it is up to standard, body weight reaches laying hen during this period
Mark explanation lays eggs persistence by force, lays eggs more；And control group body weight is not up to standard；Body weight gains are up to standard, show not employ body deposit
Laid eggs, body health state can get well, premunition enhancing.Body weight is not up to standard, illustrates laying hen and body deposit has been employed to lay eggs,
Laid eggs demand as cost meets maximum with health, premunition can be reduced.The death rate is than control group reduction by 3.1%, and 10,000 chickens are dead less
Die 310 chickens, reduces more than 9300 yuan of economic loss.
Table 6：Influence of the present invention to Layer Production Performance
| Of the present invention group | Control group | |
| Feed intake (gram) | 113.2±0.27 | 117.3±0.34 |
| Laying rate (%) | 96.55±1.24a | 93.39±1.31b |
| Egg size (gram) | 63.68±0.45a | 61.15±0.83b |
| Feedstuff-egg ratio | 1.97±0.08 | 2.25±0.15 |
| Egg production (gram/only/day) | 61.48±1.08a | 57.11±1.84b |
| Only get a profit (unit/only) | 26.95 | 21.56 |
From table 6, compared with control group, laying rate improves 3.37% to the present invention, and egg production improves 7.65%, and egg size is carried
It is high by 4.13%, being made money 5.39 yuan every chicken more.
Table 7：Influence of the present invention to laying hen immunity function and anti-oxidation function
From table 7, feed group laying hen immune function IgA, IgG, IgM of the present invention are significantly carried than control group
Height, illustrates using enhancing immunologic function threonine, methionine, vitamin A, yeast cell wall powder and yeast hydrolyate, yeast selenium
Immunity nourishment Deng series comprehensive measure is managed, and effectively enhances the immunocompetence of immunocyte, is conducive to maintaining high level
Health status, improve premunition.Present invention group glutathione peroxidase GSH-PX, hepatocuprein T-SOD, third
The Antioxidant Indexes such as dialdehyde MDA and TAC T-AOC are also significantly improved, and illustrate the day in the corn protein powder in feed
The related nutritionals such as right lutein, Hydrolysable Tannins acid, zinc methionine directly affect the Antioxidant Indexes of laying hen blood, and laying hen is anti-oxidant
Ability strengthens, and improves intestinal health state, so as to improve laying hen body health level, strengthens premunition, reduces cultivation medication.
The above description of test present invention has preferable intestinal health, egg laying performance, immunologic function strong and premunition is strong etc.
Effect, can be as the application of nonreactive daily ration and popularization.
Claims (10)
1. a kind of nonreactive laying cycle of laying hens compound premixed feed, is prepared by the raw material of following parts by weight：
Corn protein powder 5-10 parts, peanut meal 8-25 parts, rice bran 5-10 parts, sesame seed meal 15-25 parts, dietary fiber 20-50 parts, greatly
Soya-bean oil 1-10 parts, 0.5% 5 parts of compound premix, stone flour 8-15 parts, calcium monohydrogen phosphate 8-15 parts, salt 2.5-4.5 parts, lysine
1-3 parts, threonine 1-3 parts, methionine 1-3 parts, it is excellent can 0.1-0.5 parts of treasured, yeast hydrolyate 1-3 parts, yeast cell wall powder
0.3-2 parts, yeast selenium 0.1-0.5 parts, zinc methionine 0.1-0.5 parts, hemoprotein 0.2-0.5 parts, complex enzyme formulation 0.2-
0.5 part, 0.8 part of Choline Chloride, 10,000 0.2 part of unit phytases；
Characterized in that, the dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasound
Extract, biological enzymolysis after fermentation is obtained；
The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo fiber
In mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction
20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme
70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25-30 DEG C, adds the glucose of mixture quality 3-5%, 0.2-
0.5% mixed bacteria, 25-30 DEG C is cultivated 48-72 hours, and speed of agitator is 20-30r/min, and culture was added by 50-55 hours
The Cys of mixture quality 0.005-0.1% and the Radix Glycyrrhizae Ultramicro-powder of 0.05-0.1%, be concentrated under reduced pressure after fermentation ends,
Freeze-drying, low-temperature grinding are to particle diameter for 0.1-0.3mm obtains final product dietary fiber.
2. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, it is characterised in that the mixed bacteria contains
There is following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=10,000,000,000/
G, lactic acid bacteria >=5,000,000,000/g.
3. nonreactive laying cycle of laying hens compound premixed feed according to claim 2, it is characterised in that the saccharomycete is to protect
Strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016 is hidden, deposit number is CGMCC No.12789.
4. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, it is characterised in that the biology enzyme is wood
Dextranase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 uniform mixing.
5. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, it is characterised in that prepared by the onion powder
Method comprises the following steps：
Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and the mixing of onion weight 0.03% is added afterwards
Enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid under the conditions of 55 DEG C, 300W, 80KHz
Ultrasonic extraction 20min, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；
The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, xylan
Enzyme 0.5；
The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum 0.05MPa,
45-55 DEG C of triple effect, vacuum 0.04MPa.
6. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, it is characterised in that the yeast hydrolyate
Preparation method, comprise the following steps：
(1) Shaking culture
The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of culture 30h
Obtain yeast starter liquid；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentation tank cultures
Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation mediums, 30 DEG C, led to
Tolerance 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add
Plus the Cys of final concentration of 25mmol/L, total fermentation time is 50h, obtains zymotic fluid；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, dusty yeast 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；
(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is sized mixing in self-dissolving tank by 1: 1.5 mass ratio, is adjusted
Whole pH value 6-8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C of -50 DEG C of insulation self-dissolving 24h；Mixing enzyme preparation is added, is used
It is the 1%-1.5% of yeast paste quality to measure；Stirring, enzymatic hydrolysis 10h-24h；
The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase mixed in equal amounts
Form；
(5) being spray-dried 120 DEG C of -140 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.
7. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, it is characterised in that the saccharomycete is to protect
Strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016 is hidden, deposit number is CGMCC No.12789.
8. nonreactive laying cycle of laying hens compound premixed feed according to claim 1, is prepared by the raw material of following parts by weight：
8 parts of corn protein powder, 19 parts of peanut meal, 6 parts of rice bran, 20 parts of sesame seed meal, 20 parts of dietary fiber, 1.5 parts of soybean oil,
0.5% 5 parts of compound premix, 10 parts of stone flour, 12 parts of calcium monohydrogen phosphate, 3.5 parts of salt, 1.3 parts of lysine, 1 part of threonine, egg ammonia
1.5 parts of acid, it is excellent can be precious 0.2 part, 1 part of yeast hydrolyate, 0.5 part of zinc methionine, 0.5 part of complex enzyme formulation, Choline Chloride 0.8
Part, 10,000 0.2 part of unit phytases, 1 part of yeast cell wall powder, 0.3 part of yeast selenium, 0.3 part of hemoprotein.
9. the preparation method of any nonreactive laying cycle of laying hens compound premixed feeds of claim 1-8, comprises the following steps：
Each component raw material accurately is weighed according to formula, complex enzyme formulation, yeast cell wall powder, yeast hydrolyate are answered with 0.5%
Premix is closed to be pre-mixed, it is then arrogant to small by weight to sequentially add various raw materials, it is well mixed, processing granulation, i.e.,
Obtain nonreactive laying cycle of laying hens compound premixing and feed.
10. the application process of any nonreactive laying cycle of laying hens compound premixed feeds of claim 1-8：By corn, dregs of beans,
After stone flour is crushed respectively, the nonreactive laying cycle of laying hens compound premixed feed any with claim 1-8 is used after mixing；
According to following proportioning：Corn 62%, dregs of beans 20%, stone flour 8%, nonreactive laying cycle of laying hens compound premixed feed 10%.
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| CN108703261A (en) * | 2018-05-25 | 2018-10-26 | 中国农业科学院兰州畜牧与兽药研究所 | Application of the pharmaceutical composition in the drug for preparing prevention laying hen Salmonella infection disease |
| CN109953217A (en) * | 2017-12-23 | 2019-07-02 | 惠州市乐夫农业科技有限公司 | Selenium-rich function feed |
| CN111480734A (en) * | 2019-01-28 | 2020-08-04 | 安琪酵母(柳州)有限公司 | Antibacterial feed suitable for chickens and preparation method and application thereof |
| CN112205516A (en) * | 2020-09-30 | 2021-01-12 | 广西壮族自治区农业科学院 | Nutritional combined chicken feed for improving immunity of organism and preparation method thereof |
| CN113397061A (en) * | 2021-04-25 | 2021-09-17 | 康地饲料（中国）有限公司 | Premix for preventing fatty liver syndrome of laying hens |
| CN113475640A (en) * | 2021-07-20 | 2021-10-08 | 长春禾丰饲料有限责任公司 | Compound feed for reducing feed conversion ratio of broiler chickens and preparation method and application thereof |
| CN114181858A (en) * | 2021-12-09 | 2022-03-15 | 沈阳丰美生物技术有限公司 | Functional feed additive for preventing and treating necrotic enteritis of poultry and preparation method thereof |
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| CN109953217A (en) * | 2017-12-23 | 2019-07-02 | 惠州市乐夫农业科技有限公司 | Selenium-rich function feed |
| CN108703261A (en) * | 2018-05-25 | 2018-10-26 | 中国农业科学院兰州畜牧与兽药研究所 | Application of the pharmaceutical composition in the drug for preparing prevention laying hen Salmonella infection disease |
| CN111480734A (en) * | 2019-01-28 | 2020-08-04 | 安琪酵母(柳州)有限公司 | Antibacterial feed suitable for chickens and preparation method and application thereof |
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| CN113397061A (en) * | 2021-04-25 | 2021-09-17 | 康地饲料（中国）有限公司 | Premix for preventing fatty liver syndrome of laying hens |
| CN113475640A (en) * | 2021-07-20 | 2021-10-08 | 长春禾丰饲料有限责任公司 | Compound feed for reducing feed conversion ratio of broiler chickens and preparation method and application thereof |
| CN114181858A (en) * | 2021-12-09 | 2022-03-15 | 沈阳丰美生物技术有限公司 | Functional feed additive for preventing and treating necrotic enteritis of poultry and preparation method thereof |
| CN114181858B (en) * | 2021-12-09 | 2023-10-10 | 禾丰食品股份有限公司 | Functional feed additive for preventing and treating necrotic enteritis of birds and preparation method thereof |
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Application publication date: 20170531 |