CN106858132A

CN106858132A - It is a kind of to promote finishing period feed of laying hen Development of Digestive Organs and preparation method thereof

Info

Publication number: CN106858132A
Application number: CN201710006739.7A
Authority: CN
Inventors: 王玉璘; 刘敏跃; 邵彩梅; 郭耀棋; 朱秋凤; 丛玉艳; 刘燕; 金虎; 曹岩峰; 赵恒波
Original assignee: Liaoning Hefeng Animal Husbandry Co ltd
Current assignee: Liaoning Hefeng Animal Husbandry Co ltd
Priority date: 2017-01-05
Filing date: 2017-01-05
Publication date: 2017-06-20

Abstract

The invention belongs to fodder compound technical field, it is related to promote the finishing period feed and preparation method of laying hen Development of Digestive Organs.It is prepared by the main raw material by following parts by weight：45 60 parts of cereal, 8 15 parts of dregs of beans, 18 parts of miscellaneous dregs of rice class, 6 15 parts of bran, 38 parts of pomace class, 2 parts of the oenothera biennis dregs of rice, 0.8 1.5 parts of stone flour, 0.8 1.5 parts of calcium monohydrogen phosphate, 0.35 0.43 parts of amino acid, 0.5 1.5 parts of vegetable oil, 1 part of compound premix, 0.05 0.2 parts of prebiotics, 0.01 0.05 parts of zinc methionine, 0.05 0.2 parts of Hydrolysable Tannins acid, 0.05 0.08 parts of complex enzyme formulation, 0.02 0.1 parts of mold toxin sorbent, 0.04 0.2 parts of degrading mold toxin enzyme, 0.1 0.5 parts of yeast hydrolyate, 15 parts of dietary fiber.Each digestive organs indices of laying hen finishing period are made to have raising in various degree.

Description

It is a kind of to promote finishing period feed of laying hen Development of Digestive Organs and preparation method thereof

Technical field

The invention belongs to fodder compound technical field, and in particular to a kind of finishing period of promotion laying hen Development of Digestive Organs Feed, and it is related to the preparation method of the feed.

Background technology

Laying hen finishing period feed is exactly the feed that laying hen uses from 7 week old -15 week old stages, stage physiological function hair Educate immature, bone, muscle and digestive organs are in the vigorous stage of development, and this period does not lay eggs, and raiser thinks little of, The Growing Chicken field corn that uses, dregs of beans are few, and the miscellaneous dregs of rice such as corn bran, corn DDGS etc., palm kernel meal, Cottonseed Meal is more, causes Huang Aspertoxin is exceeded serious, destroys the normal equilibrium of gut flora.The miscellaneous dregs of rice fiber quality such as palm kernel meal and corn byproducts is bad, There is certain damage to intestinal villus, had a strong impact on this stage intestinal condition.The Growing Chicken stage needs to temper digestive function, promotees Enter muscular stomach, glandular stomach, intestinal growth, it is desirable to which enteron aisle length is long, muscular stomach glandular stomach weight weight could lay eggs phase digestion ability in the later stage By force, intestinal health is good, is that the performance of laying hen all one's life egg laying performance is laid a good foundation.

Mainly have to the research of laying hen finishing period feed it is following these：

Publication number：A kind of laying hen finishing period complete feed of the disclosure of the invention of 103349179A, is by following weight portion Component composition：Corn 350-450 parts, wheat 150-200 parts, dregs of beans 50-100 parts, peanut meal 10-40 parts, cotton dregs 30-40 Part, rapeseed meal 10-30 parts, maize gluten feed 40-60 parts, 40-80 parts of DDGS (vinasse), rice bran meal 70-150 parts, stone flour 15-25 Part, bone meal 10-25 parts, soya-bean oil 5-6 parts, calcium monohydrogen phosphate 6-10 parts, salt 2.5-3 parts, sodium sulphate 1-2 parts, choline 0.5-1 parts, 2 parts of composite trace element, lysine 2-5 parts, methionine 1-1.5 parts, 1.3 parts of additive.Chicken finishing period full price of the invention is raised Material, low cost, after feeding laying hen, laying hen opens antenatal body weight and can reach or more than breed standard, and body maturation is synchronous with sexal maturity.But Be corn bottle opener use it is more, the possibility that there is aflatoxin is larger, do not pay close attention to be bred as laying hen intestinal health.

Publication number：A kind of Pullet special feed of the disclosure of the invention of 103689250A, the feed is by following weight The raw material of percentage is obtained：Corn 150-180 parts, 35-40 parts of sorghum wheat-middlings, dregs of beans 45-50 parts, paddy 50-60 parts, rice bran 45-50 parts, 55-60 parts of the palm kernel dregs of rice, peanut straw 45-50 parts, Os Sus domestica powder 2-3 parts, zeolite 1-2 parts, pawpaw seeds 3-4 parts, fish scale Powder 1-2 parts, Kelp Powder 2-3 parts, 4-5 parts of small chrysanthemum cauliflower, mulberry 5-6 parts, does 2-3 parts of silverfish powder, butter 2-3 by lalang grass rhizome 4-5 parts Part, 2-3 parts of bean worm powder, bitter dish 3-4 parts, proper amount of salt, phagostimulant 4-5 parts.The unconventional feed that the invention is used is more, Relatively costly, application value less, and does not pay close attention to the intestinal health for being bred as laying hen.

Publication number:The invention of 103099063A《A kind of laying hen finishing period feed》A kind of laying hen finishing period feed is disclosed, It is made up of the component of following weight portion：Di-calcium phosphate 80-150 parts, stone flour 250-350 parts, salt 40-60 parts, little Su Beat 20-40 parts, choline 12-20 parts, composite trace element 30-40 parts, B B-complex 8-10 parts, methionine 20-25 parts, bad ammonia It is sour 15-20 parts, bone meal 100-150 parts, 100-200 parts of lysine slag, zeolite powder 50-150 parts, Nosiheptide 1-3 parts, Robenidine 1- 3 parts, phytase 2-5 parts, complex enzyme formulation 2-4 parts, xylo-oligosaccharide 2-3 parts.Feed of the invention is obtained by putting into practice for many years , be added to nutritive additive etc. in feed by the present invention, and the finishing period feed produced can make age in days of laying hen 110 or so Body weight is up to standard, sexal maturity is synchronous with body maturation, the uniformity more than 80%.The invention has used Multiple Classes of Antibiotics, has antibiotic residual The risk stayed, and do not pay close attention to the intestinal health for being bred as laying hen.

Publication number:The invention of 101822236A《A kind of method for breeding of correcting dysplasia of laying hens at growing period》The method exists Laying hen finishing period corrects phase feed and auxiliary corrective phase feed as laying hen finishing period feed using emphasis, regulates and controls laying hens in brooding period The body type indicators at end, laying hen finishing period fodder energy level is 2800 kilocalories/kg-2850 kilocalories/kg, and protein level is 16.0%-19.0%, emphasis correction phase feed is used for 7-12 week old layer dietses, and auxiliary corrective phase feed is used for 9-18 week old Layer dietses；Wherein：The emphasis correction phase, feed was graininess, and its raw material weight and its percentage are：Corn：59.2-62.6%, Dregs of beans：17-19%, rapeseed dregs：4-6%；Corn protein powder：5.4-6.8%, fish meal：1-3%, wheat bran：0.5-3%, plant Oil：1.5-2.3%, garlic powder：0.3-0.5%, methionine：0.1-0.2%, lysine：0.08-0.12%, Laying chicks mineral, Compound vitamin additive：4-6%, the percentage sum of above-mentioned raw materials is 100%；Auxiliary corrective phase feed is graininess or powder Shape, its raw material weight and its percentage are：Corn：60.0-70.0%, dregs of beans：5-12%, rapeseed dregs：2.0-6.0%；Cottonseed The dregs of rice：2.0-6.0%, dusty yeast：1.0-3.0%, fish meal：0.5-3.0%, wheat bran：8.0-9.0%, garlic powder：0.2-0.4%, Methionine：0.05-0.15%, lysine：0.08-0.12%, Laying chicks mineral, compound vitamin additive：2.0-3.0%, The percentage sum of above-mentioned raw materials is 100%；

Publication number:The invention of 106036171A《A kind of preparation method of laying hen finishing period feed》Disclose following content： The preparation method of laying hen finishing period feed, weighs the raw material of following weight portion：Corn 60-62, feather meal 20-25, wheat bran 18- 24th, vegetable oil 0.5-1, salt 0.3-0.6, B B-complex 0.1-0.5, trace element 0.3-0.6, GABA 1.5- 2.5th, Traditional Chinese drug microecology microcapsules 8-10, after corn, wheat bran are crushed, adds remaining material, is cooled down through high temperature granulating, prepared Grain feed.It is main to replace antibiotic using Chinese herbal medicine, medicament residue problem, Growing Chicken are effectively reduced during animal feeding The problem of survival rate, the uniformity and resisting stress, and biofermentation traditional Chinese medicine technology is utilized, the drug effect of Chinese herbal medicine is improve 4-20 Times, it is more obvious in prevention disease and the effect for improving the aspects such as production performance.But trophic component is not balanced enough, to being bred as egg The concern of chicken intestinal health is inadequate.

Enteron aisle is the main place of absorption of nutrient ingredients, and small bowel is divided into stratum supravasculare, submucosa, muscle layer and serous coat Layer.The BBM of enterocyte has special construction and function, and protein is " doorbell " and " door on epithelial cell on film Family ", is responsible for digesting and assimilating for nutriment, recognizes the signal in extracellular stimulation and active cell.With enterocyte Differentiation, their expression on film also there occurs change.

Induced by lipopolysaccharide (LPS) is produced in enteron aisle harmful bacteria more, by with host animal intestinal epithelial cell film Toll-like receptor interaction produces bad immune response and causes infectious diseases to induce.Addition amino-acid zinc can promote egg The development of chicken spleen, strengthens cellular immunity function；Poison is attacked by LPS and triggers immune response, addition amino-acid zinc can mitigate laying hen Immunization inflammatory reaction；The detoxification mechanism of induced by lipopolysaccharide (LPS) is by the lipoid A function groups in their macromoleculars, hair Intoxicating effect is waved, and this lipoid A function groups have two inorganic phosphate radical ions, and via enzyme hydrolysis to the endotoxic class The double phosphorus key hydrolysis of fat A function groups, then toxicity subtracts greatly lipoid A function groups induced by lipopolysaccharide (LPS) that hydrolysis dephosphorization is produced, Can not effectively with the Toll-like receptor interaction of epithelial cell membrane and produce adverse immune react and cause a disease.

The finishing periods such as corn byproducts are often used in raw material, the AFB measured using enzyme linked immunosorbent assay₁Plain 5- 100 μ g/kg, vomitoxin 300-2000 (μ g/kg), zearalenone 80-800 (μ g/kg), Trichothecenes toxin etc. A large amount of toxin；Aflatoxin is hepatotoxin, and the hepatic injury of low dosage actuatable thing, immunity function suppress, and Long Term Contact can trigger Animals and humans canceration is lethal.Trichothecenes toxin belongs to greatly the tissue stimulation factor and inflammation-causing substance, can coup injury Animal digestion mucous membrane；Poisoning symptom normally behaves as anorexia or useless exhausted, gastrointestinal inflammation and bleeding, vomiting, diarrhoea etc..It is beautiful The clinical symptoms of Zearlenone poisoning include that feed conversion rate reduction, organ weight's change, animal fertility decline and behavior It is abnormal etc..Using biologic detoxication enzyme edman degradation Edman, degraded toxin specificity, the nutritive value of feed is not influenceed, the storage of toxin is avoided Product contact scar environment, the toxic action for avoiding secondary metabolite.

Dietary fiber is the framework ingredient of plant cell in daily ration, and the enzyme hydrolysis to animal digests resistant in small intestine The carbohydrate not being digested inside, microorganism is turned into rear intestinal can utilize the material of fermentation.Dietary fiber is (indigestible Carbohydrate and lignin).Fiber is a kind of complicated mixture, and aqueous soluble dietary can be divided into according to water miscible difference Fiber (SDF) and water insoluble dietary fiber (IDF)；Vegetable diet fiber, animality meals can be divided into according to source difference Food fiber and synthesis class dietary fiber.In recent years again there is scholar to propose, dietary fiber should be divided into can fermented type dietary fiber and Can not fermented type dietary fiber.Water insoluble dietary fiber is mainly made up of plant cell wall, and such as cellulose, lignin and half are fine Dimension element, is present in wheat, most of cereal and vegetables.It can promote intestines peristalsis, reduce delay of the food in enteron aisle Time.Water-soluble dietary fiber is made up of Hemicellulose Polysaccharide, is such as present in the pectin in fruit, oat, barley, beans and coagulates Glue etc..It is generally hydrophilic, and non-crystalline type fiber, the metabolism with carbohydrate and lipid is relevant, is easily soaked by enteron aisle liquid, because This is easy to butyric acid and butyric acid producing strains in the human body by the Institute of Micro-biology in enteron aisle using intake high dietary-fibers and substantially increases Many, illustrating the intake of high dietary-fiber can promote the generation of SCFA and healthy intestinal flora.Research is it has also been found that find paddy Thing dietary fiber --- pentosan can be degraded by the bacterium with relevant enzyme in enteron aisle, therefore can breed Bifidobacterium and newborn bar The beneficial bacteriums such as bacterium.The research of Xie Chunyan etc. shows, lactic acid bacteria is given birth to soluble dietary fiber obtained in agrocybe aegerita fermentation wheat bran It is long that there is proliferation function but then inhibited to the growth of Escherichia coli.

Application and function mechanism of the crude fibre in nonruminant diet is as follows：One is the steady of maintenance intestinal microecology Fixed, nonruminant enteron aisle lacks the coarse-fibred lignocellulose degradation enzyme that can degrade, therefore is unable to digestibility and utilization overwhelming majority species Crude fibre, but in crude fibre can fermented ingredient but can be to be utilized by bacterial degradation in enteron aisle, serve as its energy source.And Some compositions in crude fibre can only be decomposed by intestinal beneficial bacterium and utilized, and can not but be utilized by harmful bacteria, so that can be certain Promote the growth of beneficial bacterium in degree, beneficial to the balance of intestinal microecology, prevent animal digestion function from getting muddled.Jones (2013) correlation between swine rationses fiber and intestinal environment, enteric microorganism and host is illustrated.Food fiber exists Into after small intestine, become polysaccharide and then be further decomposed into carbohydrate and oligosaccharide.And oligosaccharide can be enteric cavity and intestinal mucosa layer it is beneficial Bacterium provides nutriment, and the bacterium and its metabolite of enteric cavity and mucous layer are to maintaining the normal physiological function of enteron aisle and signal Conduction plays the role of positive.Therefore, crude fibre can to a certain extent safeguard that intestinal environment, intestinal microecology and its signal are passed That leads is normal.Two is to stimulate intestines peristalsis, and the wriggling of animal intestinal tract absorbs nutriment, formed and discharge excrement for animal digestion Just there is highly important effect.Three is absorption harmful substance, and certain toxin is contained in usual animal intestinal tract chyme, and it may From feed (mycotoxin), gut metabolism product or harmful microorganism.These toxin can deposit if it can not remove in time The stabilization of enteron aisle, destruction intestinal mucosa and intestinal microecology.And crude fibre is it to the harmful effects of animal intestinal tract toxin is reduced His material is incomparable.It is by combining, isolating, adsorb and decorporation 4 that crude fibre is adsorbed in enteron aisle and discharges harmful substance What individual effect was completed.Different coarse fiber contents mechanism of action of absorbing toxin in enteron aisle is different, but it is all high Effect, enteron aisle " antidote " nontoxic and without ill-effect.

Breeding layer chicken is at all intestinal health, safeguards the normal physiological condition of enteron aisle and reduces wound of the toxin to enteron aisle Evil, crude fibre can alleviate to a certain extent even remove enteron aisle in toxin and it is nontoxic have no adverse reaction, this to reduce medicine Play the role of in the use of nonruminant body with antibiotic positive.

In sum, according to Pullet digestive organs physiological development feature, the enteron aisle battalion of Development of Digestive Organs is promoted Support from amino acid nutrient, such as threonine and arginine, the additive such as probiotics, enzyme preparation, degrading mold toxin agent and mould poison Plain adsorbent etc. reduces the harm such as aflatoxin and vomitoxin, strengthens the aspects such as crude fibre nutrition and sets about solving, and is have Certain effect, a kind of comprehensive nutrition of development, security are good, effectively facilitate the laying hen incubation of Development of Digestive Organs, intestinal growth Phase feed is extremely urgent.

The content of the invention

Present invention solves the technical problem that one be in effectively mitigating finishing period feed aflatoxin, vomitoxin etc. to intestines The damage in road；Two is, using suitable rational fiber nutrient, to promote Development of Digestive Organs；Three is using amino acid and probiotics Gut flora balance is strengthened in nutrition, reaches the mesh such as laying hen digestive organs muscular stomach and glandular stomach weight weight, enteron aisle intestinal villus crypts depth long Mark, a kind of finishing period feed of promotion laying hen Development of Digestive Organs of science compounding.

To reach above target, the present invention uses following technical scheme：

A kind of finishing period feed of promotion laying hen Development of Digestive Organs, is prepared by the raw material of following parts by weight：

Cereal 45-60 parts, dregs of beans 8-15 parts, 1-8 parts of miscellaneous dregs of rice class, bran 6-15 parts, 3-8 parts of pomace class, corn bran 2-5 Part, 2 parts of the oenothera biennis dregs of rice, stone flour 0.8-1.5 parts, calcium monohydrogen phosphate 0.8-1.5 parts, salt 0.2-0.5 parts, amino acid 0.35-0.43 Part, vegetable oil 0.5-1.5 parts, 1 part of compound premix, prebiotics 0.05-0.2 parts, zinc methionine 0.01-0.05 parts, hydrolysis is single Peaceful 0.05-0.2 parts of acid, complex enzyme formulation 0.05-0.08 parts, mold toxin sorbent 0.02-0.1 parts, degrading mold toxin enzyme 0.04-0.2 parts, yeast hydrolyate 0.1-0.5 parts, dietary fiber 1-5 parts；

Further, the cereal is at least one of corn, barley, wheat, oat；

Further, the miscellaneous dregs of rice class is at least one of Cottonseed Meal, peanut meal, rapeseed dregs；

Further, the bran is at least one of rice bran meal, wheat bran, oat bran；

Further, the pomace class is at least one of pomace, citrus pulp, treaster；

Further, the prebiotics is at least one of manna oligosacchride, isomalto-oligosaccharide, xylo-oligosaccharide；

Further, the amino acid preferably mass ratio is：Lysine：Threonine：Methionine=1:0.44- 0.63:0.67-0.88；

Further, the dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasound Extract, biology enzyme enzymolysis after fermentation is obtained；

The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme 70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25-30 DEG C, adds the glucose of mixture quality 3-5%, 0.2- 0.5% mixed bacteria, 25-30 DEG C is cultivated 48-72 hours, and speed of agitator is 20-30r/min, and culture was added by 50-55 hours The Cys of mixture quality 0.005-0.1% and the Radix Glycyrrhizae Ultramicro-powder of 0.05-0.1%, be concentrated under reduced pressure after fermentation ends, Freeze-drying, low-temperature grinding are to particle diameter for 0.1-0.3mm obtains final product dietary fiber；

The mixed bacteria contains following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >= 100000000000/g, saccharomycete >=10,000,000,000/g, lactic acid bacteria >=5,000,000,000/g；

Preferably, the saccharomycete is saccharomyces cerevisiae；

Further, the saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) Tlj2016, deposit number is CGMCC No.12789；

The biology enzyme is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 is uniform Mixing；

Further, the onion powder, preparation method thereof comprises the following steps：

Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and onion weight 0.03% is added afterwards Mixing enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid in 55 DEG C, 300W, 80KHz bar Ultrasonic extraction 20min under part, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；

The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, wood Dextranase 0.5；

The vacuum concentration, specially：One 75-85 DEG C of effect, vacuum 0.07MPa, two 65-75 DEG C of effects, vacuum 0.05MPa, 45-55 DEG C of triple effect, vacuum 0.04MPa.

Further, the preparation method of the yeast hydrolyate, comprises the following steps：

(1) Shaking culture

The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of trainings Foster 30h obtains yeast starter liquid；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 35, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1, pH6.0；

(2) 5L fermentation tank cultures

Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed and is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/min, tank pressure 0.03MPa, 500rpm carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, once Property addition final concentration of 25mmol/L Cys, total fermentation time is 50h, obtains zymotic fluid；

Fermentation medium (g/L)：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、KH₂PO₄0.5th, dusty yeast 11、MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1,pH6.0。

(3) yeast paste is prepared：Zymotic fluid obtained in step (2) is carried out into separation of solid and liquid by centrifuge, yeast paste is collected；

(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio Slurry, adjusts pH value 6~8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C~50 DEG C insulation self-dissolving 24h；Add mixed enzyme Preparation, consumption is the 1%~1.5% of yeast paste quality；Stirring, enzymatic hydrolysis 10h~24h.

The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase equivalent Mix；

(5) being spray-dried 120 DEG C of -140 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.

Preferably, the yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, protect It is CGMCC No.12789 to hide numbering.

It is including following present invention simultaneously provides a kind of preparation method of the finishing period feed for promoting laying hen Development of Digestive Organs Step：

Each component raw material accurately is weighed according to formula, corn, dregs of beans, barley, miscellaneous dregs of rice class, bran are sieved by 6.0mm Piece is crushed according to conventional egg feedstuff processing method, and complex enzyme formulation, prebiotics and compound premix are pre-mixed, and is then pressed Parts by weight are arrogant to small to sequentially add various raw materials, is well mixed, processing granulation, obtains final product and promotes laying hen Development of Digestive Organs Finishing period feed.

Beneficial effect

The present invention according to Pullet digestive organs physiological development feature, promote the enteral nutrition of Development of Digestive Organs from The additives, degrading mold toxin agent such as amino acid nutrient, such as threonine and arginine, the beneficial factor, enzyme preparation and Hydrolysable Tannins acid The harm such as aflatoxin and vomitoxin is reduced with mold toxin sorbent etc., is strengthened the aspects such as crude fibre nutrition and is set about solving Certainly, muscular stomach improves 9.3% with glandular stomach index, and index and spleen index improves 46%；Duodenum and small intestine weight are respectively increased 33.6% and 22.1%, duodenum length and small intestinal length have been respectively increased 33.4% and 28.6%.Digestive organs and immune Organ is significantly better than control group.Fallopian tubal length and weight are all significantly improved；As can be seen here, the present invention is promoting digestive organs Development, intestinal growth aspect effect are very notable.Field observation is visible, and laying hen build is delicate and pretty, rounded, being full of animal spirits, table Reveal the chicken group character of stalwartness.

Especially with dietary fiber prepared by ad hoc approach, soluble dietary fibre content is high, bioactivity is strong, and protects A number of Water insoluble dietary fiber is stayed, has been coordinated with prebiotics, gut flora can have been significantly improved, improved enteron aisle benefit The resident time of the quantity of raw flora, regulation and maintenance beneficial bacteria of intestinal tract group, strengthens digestion and the absorbability of enteron aisle, improves dynamic Thing immunity.The preparation of dietary fiber, enzymolysis, ultrasonic extraction is combined with superfine communication technique, using specific technique bar Part, while soluble dietary fibre content is improved, and can improve soluble dietary fiber and Water insoluble dietary fiber Characteristic, its retentiveness, dilatancy, thickening property have raising in various degree.Dietary fiber, onion are prepared from onion powder：Nature and flavor Pungent-warm, sweet delicate, in addition to containing common nutriment, prostaglandin A and sulphur Amino acid contained by it have expansion of blood vessels, Regulation blood fat, prevents the effect of artery sclerosis, assigns dietary fiber of the present invention the key property different from similar dietary fiber.

Prepared by yeast hydrolysate is prepared using special process, the content of glutathione can be significantly improved, glutathione has anti- The functions such as oxidation, removing free radical, removing toxic substances, strengthen immunity, anti-aging, anticancer, the harm of anti-radiation line, are important functions The factor, can improve the skin health of chick, and feather glossiness, the work with enhancing immunologic function, enriched nutritive With.Acted synergistically with prebiotics etc., the propagation of beneficial bacterium can be promoted, improve the development of chick intestinal villi, reduce diarrhea rate, carried Animal immunizing power high；The preservation of bacteria strain tlj2016 of high yield glutathione is particularly added, glutathione can be more significantly improved and be contained Amount, after fermentation ends, the content of GSH can reach 3308mg/L in zymotic fluid, and glutathione is used as important anti-oxidant in vivo Agent and free radical scavenger, are such as combined with free radical, heavy metal, the poisonous substance being harmful in body can be converted into harmless thing Matter, excretes external, the physical function to improving incubation laying hen, very helpful.

Specific embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change Belong to protection scope of the present invention.

The Wine brewing yeast strain is specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterium Strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number on July 15th, 2016 It is CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research Institute, postcode 100101.

The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；

The saccharomyces cerevisiae tlj2016 is isolated saccharomyces cerevisiae starting strain from the orchard of one plant of Ningxia through following Step is obtained：

The original strain that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → hypertonic plate screening → nitrosoguanidine (NTG) secondary screening (producing glutathione GSH abilities) → passage stabilization is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask Property experiment.

By the bacterial strain tlj2016 obtained after mutagenesis, its glucose tolerance and Cys tolerance are equal It is improved, cell density, another aspect Cys tolerance energy on the one hand can be improved under high concentration glucose culture The raising of power is beneficial to GSH and largely synthesizes in intracellular, so as to improve the ability that bacterial strain mass produces GSH.

Saccharomyces cerevisiae of the present invention reaches 300g/L to the tolerance of glucose, beneficial to it in high concentration grape GSH is produced under the conditions of sugar；3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；Tolerate the energy of Cys Power is far above starting strain, slow growth is remained under the effect of 5mmol/L Cys, in 40mmol/LL- cysteines Remain to keep GSH largely to synthesize under effect；Salt resistance ability reaches 18%, is conducive to extending its application field.

1.DES mutagenic and breedings

1) ring of starting strain 1 one on test tube slant is taken on super-clean bench, is accessed equipped with 50mL malt extract mediums In 250mL triangular flasks, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.

2) 5mL bacterium solutions are taken, 5000rpm centrifugation 10min collects thallines, with brine 2 times.

3) 10 are diluted to pH7.0 phosphate buffers⁷Individual/mL bacteria suspensions.

4) kaliumphosphate buffer of 32mL pH7.0,8mL bacteria suspensions, 0.4mL DES are taken and is being placed in advance in the 150mL of rotor It is sufficiently mixed in triangular flask, makes DES ultimate densities be 1% (v/v).

5) the 150rpm reactions 30min in 30 DEG C of shaking tables, takes 1mL mixed liquors, adds 0.5mL 25%Na₂S₂O₃In solution Only react.

6) dilution spread is in the malt extract medium plate containing 150g/L KCl, the picking bacterium after 30 DEG C of cultures 2-3 days The bacterial strain 1 of the maximum that falls.

2. nitrosoguanidine mutagenesis

1) ring of saccharomyces cerevisiae bacteria strain 1 one on test tube slant is taken on super-clean bench, is accessed and 50mL brewer's wort cultures is housed In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 10h or so, thalline is in logarithmic growth early stage.

2) 5mL bacterium solutions 5000rpm centrifugation 10min collects thallines are taken, with brine 2 times.

3) 10 are diluted to pH6.0 phosphate buffers⁷Individual/mL bacteria suspensions.

4) 10mL bacteria suspensions are taken to be transferred in 100mL triangular flasks, the NTG of 10mg is added, final concentration of 10mg/mL is configured to NTG solution, and add 4-5 drop acetone, be beneficial to NTG dissolvings.

5) the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm are centrifuged 10min collects thallines, use SPSS Wash for several times, stopped reaction.

6) appropriate dilution spread, takes the bacterium solution 0.2mL of last dilution factor, coats the brewer's wort culture containing 200g/L KCl In base plate.Picking colony 20 after being cultivated 2-3 days at 30 DEG C.

3. shaking flask primary dcreening operation

1) take above-mentioned each ring of 20 S. cervisiaes respectively on super-clean bench, be respectively connected to equipped with 50mL brewer's wort cultures In the 250mL triangular flasks of base, 200rpm, 30 DEG C are cultivated 12h or so, thalline is in mid log phase.

2) 5mL bacterium solutions are taken, is accessed equipped with the 250mL in the hypertonic malt extract mediums of 50mL (concentration of glucose is 300g/L) In triangular flask, 200rpm, 30 DEG C are cultivated 3-4 days, daily detection concentration of glucose and concentration of alcohol change.After fermentation ends, than Compared with 20 plants of glucose and ethanol wear rate of strain, final remaining sugar concentration and concentration of alcohol, glucose to the conversion ratio of ethanol And heteroacid content.

3) glucose consumption rate is fast, final remaining sugar concentration is low and concentration of alcohol is high 5 plants of bacterium are named as Y-1, Y- for selection 2, Y-3, Y-4, Y-5.

4. ferment secondary screening

By 5 plants of bacterium Y-1, Y-2, Y-3, Y-4, Y-5 and starting strain of gained in step 3, respectively according to 10% inoculation Amount, the 150rpm in the 250mL shaking flasks equipped with 30mL fluid nutrient mediums, 30 DEG C of culture 30h, takes zymotic fluid and determines GSH concentration；

Fluid nutrient medium (g/L)：(NH₄)₂SO₄6th, glucose 35, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1；

GSH assay methods：Fresh yeast, at 30 DEG C, 40% Ethanol Treatment 3h, in centrifuging and taking are obtained after zymotic fluid centrifuge washing Clear liquid is cooked GSH determination samples；

GSH measure is carried out using alloxan method：Its principle is that-the SH on GSH reacts with alloxan, the material of generation There is absworption peak at 305nm, and it is linear with glutathione concentrations, therefore can be quantitative determined with ultraviolet specrophotometer GSH contents.

Table 1：GSH testing results

Bacterial strain	Starting strain	Y-1	Y-2	Y-3	Y-4	Y-5
							GSH concentration (mg/L)	127.9	195.7	172.3	263.2	216.5	185.2

As seen from the results in Table 1, bacterial strain Y-3 has highest GSH fermentabilities, it is thus determined that Y-3 is final production bacterium Strain, and it is named as tlj2016.

5. genetic stability experiment

Continuous ten passages on inclined-plane by tlj2016 bacterium, and detect the hair after passage every time with the method for shaking flask secondary screening Ferment situation.Experiment finds that continuous ten passages on inclined-plane, the strain proterties does not have significant change, and property indices are all just Often, illustrate that the genetic stability of the strain is stronger.

Fermented under the conditions of saccharomyces cerevisiae tlj2016 sugar high and produce GSH capacity experimentals

(1) Shaking culture

The ring of tlj2016 slant strains one is taken, 150rpm, 30 DEG C in the 250mL shaking flasks equipped with 30mL Shake flask mediums is accessed Culture 30h obtains seed liquor；

(2) 5L fermentation tank cultures

By seed liquor by the inoculum concentration of 10% mass percent, access and be equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, Throughput 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably The Cys of final concentration of 25mmol/L are added, total fermentation time is 50h；

Fermentation medium (g/L)：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、KH₂PO₄0.5th, dusty yeast 11、MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1,pH6.0；

After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.

Saccharomyces cerevisiae tlj2016L- cysteines tolerance is tested

By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to 150rpm in shaking flask, 30 DEG C are cultivated, when culture is to 12h, to the Guang ammonia of L- half that different final concentrations are added in shaking flask Acid, is further cultured for 10h, determines dry cell weight, as a result table 2,3；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 20, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1, pH6.0；

Table 2：Starting strain Cys tolerance

Cys concentration mmol/L	0	5	10	15	20	40
							Starting strain dry weight g/L	22.6	15.7	10.2	4.3	2.2	0.8
GSH concentration (mg/L)	35.6	46.7	43.2	40.7	37.9	25.3

Table 3：Tlj2016L- cysteine tolerances

Cys concentration mmol/L	0	5	10	15	20	40
							Tlj2016 dry weights g/L	25.7	28.5	23.6	21.2	20.6	18.7
GSH concentration (mg/L)	73.2	98.3	113.5	121.7	127.5	135.8

From the results shown in Table 2, for starting strain, Cys are added in culture medium, cell stops growing, And start self-dissolving, cause GSH growth rates to be reduced with the rising of Cys concentration；From the results shown in Table 3, Under low concentration Cys, tlj2016 still is able to slow growth, with the raising of Cys concentration, tlj2016 bacterial strains Dry cell weight slowly decline, and GSH concentration sustainable growths, before this result is beneficial in GSH production processes by addition Body amino acid-Cys promote the production of GSH.

Saccharomyces cerevisiae tlj2016 salt resistance abilities are tested

Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients be 0%, 2%, 5%, 10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), 24h is cultivated at being placed in 30 DEG C respectively, each treatment 3 Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions solid in YPD It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat Bacterium number number on plate.The results are shown in Table 4, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring Survived in border, still there is vigor under high salt conditions, can be applied to consume sugar in the high salt food processing process such as soy sauce, curing food Produce glutathione.

Table 4：Salt resistance ability detection (× 10⁷cfu/ml)

NaCl contents

0%

2%

5%

10%

15%

18%

Original strain

5.16±0.42

4.38±0.42

2.15±0.21

0.12±0.11

0

tlj2016

5.33±0.28

5.10±0.71

4.83±0.42

3.98±0.33

2.57±0.48

0.83±0.15

Embodiment 1

45 parts of corn, 15 parts of barley, 8 parts of dregs of beans, 3 parts of Cottonseed Meal, 2 parts of rapeseed dregs, 5 parts of oat bran, 3 parts of wheat bran, mandarin orange 2 parts of tangerine slag, 3 parts of treaster, 2 parts of the oenothera biennis dregs of rice, 1.1 parts of stone flour, 1.2 parts of calcium monohydrogen phosphate, 0.35 part of salt, 0.1 part of threonine, 0.15 part of methionine, 0.18 part of lysine, 1.2 parts of vegetable oil, 1 part of compound premix, 0.1 part of prebiotics, zinc methionine 0.05 Part, 0.05 part of complex enzyme formulation, 0.1 part of degrading mold toxin enzyme, 3 parts of corn bran, 0.1 part of Hydrolysable Tannins acid, mycotoxin is inhaled Attached dose 0.05 part, 0.3 part of yeast hydrolyate, 1 part of dietary fiber；

The prebiotics is manna oligosacchride, isomalto-oligosaccharide, xylo-oligosaccharide with 1：2：1 ratio mixing；

The dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasonic extraction, life Thing enzymolysis after fermentation is obtained；

The dietary fiber preparation method is comprised the following steps：

By onion powder, barley fiber, common oats fibre, wheat embryo fiber in mass ratio 1:3:8:3 uniform mixing, add mixed The water that 8 times of compound quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min is 5.5 with newborn acid for adjusting pH value, adds mixing The biology enzyme of amount of substance 0.2%, 45min is digested in 50 DEG C, and go out enzyme；70 DEG C of enzymolysis liquid, 18-20MPa homogeneous, material is cooled to 28 After DEG C, the glucose of addition mixture quality 4%, 0.4% mixed bacteria, 28 DEG C are cultivated 60 hours, and speed of agitator is 25r/ Min, culture to 52 hours Cys and 0.1% Radix Glycyrrhizae Ultramicro-powder of addition mixture quality 0.05%, fermentation ends Afterwards be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.2mm obtains final product dietary fiber；

The saccharomycete is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, and preservation is compiled Number be CGMCC No.12789；

The onion powder, preparation method thereof comprises the following steps：

The preparation method of the yeast hydrolyate, comprises the following steps：

(1) Shaking culture

(2) 5L fermentation tank cultures

(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio Slurry, adjusts pH value 7, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 45 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption It is the 1.2% of yeast paste quality；Stirring, enzymatic hydrolysis 18h.

(5) being spray-dried 130 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.

The yeast is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number It is CGMCC No.12789.

Promote the finishing period feed producing method of laying hen Development of Digestive Organs, comprise the following steps：Claim according to formula is accurate Each component raw material is taken, corn, dregs of beans, barley, miscellaneous dregs of rice class, bran are processed by 6.0mm sieves according to conventional egg feedstuff Method is crushed, and complex enzyme formulation, prebiotics and compound premix are pre-mixed, then arrogant to small by weight to add successively Enter various raw materials, be well mixed, processing granulation obtains final product the finishing period feed for promoting laying hen Development of Digestive Organs.

Embodiment 2

40 parts of corn, 10 parts of oat, wheat 10,10 parts of dregs of beans, 3 parts of peanut meal, 5 parts of oat bran, 3 parts of rice bran meal, oranges and tangerines 2 parts of slag, 3 parts of pomace, 2 parts of the oenothera biennis dregs of rice, 1.1 parts of stone flour, 1.2 parts of calcium monohydrogen phosphate, 0.35 part of salt, 0.1 part of threonine, egg 0.14 part of propylhomoserin, 0.16 part of lysine, 1.2 parts of vegetable oil, 1 part of compound premix, 0.1 part of prebiotics, 0.05 part of zinc methionine, 0.05 part of complex enzyme formulation, 0.1 part of mold toxin sorbent, 0.05 part of Hydrolysable Tannins acid, 2 parts of corn bran, degrading mold toxin 0.04 part of enzyme, 0.5 part of yeast hydrolyate, 3 parts of dietary fiber；

The prebiotics is manna oligosacchride；

The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme 70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25 DEG C, adds the glucose of mixture quality 5%, 0.5% mixing Strain, 25 DEG C are cultivated 72 hours, and speed of agitator is 30r/min, culture to 50 hours L- of addition mixture quality 0.005% half Cystine and 0.1% Radix Glycyrrhizae Ultramicro-powder, after fermentation ends be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.1mm is Obtain dietary fiber；

The onion powder, preparation method thereof comprises the following steps：

(1) Shaking culture

(2) 5L fermentation tank cultures

Fermentation medium (g/L)：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、KH₂PO₄0.5th, dusty yeast 11、MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1、pH6.0。

(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio Slurry, adjusts pH value 6, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption It is the 1.5% of yeast paste quality；Stirring, enzymatic hydrolysis 10h.

(5) being spray-dried 120 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.

A kind of finishing period feed producing method for promoting laying hen Development of Digestive Organs is with embodiment 1.

Embodiment 3

40 parts of corn, 20 parts of oat, 12 parts of dregs of beans, 3 parts of peanut meal, 3 parts of oat bran, 3 parts of wheat bran, 5 parts of citrus pulp, the moon See 2 parts of the careless dregs of rice, 1.1 parts of stone flour, 1.2 parts of calcium monohydrogen phosphate, 0.35 part of salt, 0.1 part of threonine, 0.14 part of methionine, lysine 0.16 part, 1.2 parts of vegetable oil, 1 part of compound premix, 0.2 part of prebiotics, 0.05 part of zinc methionine, 0.05 part of complex enzyme formulation, 0.1 part of mold toxin sorbent, 0.1 part of Hydrolysable Tannins acid, 5 parts of corn bran, 0.2 part of degrading mold toxin enzyme, yeast hydrolyate 0.1 part, 5 parts of dietary fiber；

The prebiotics is that mass ratio is 1：1 isomalto-oligosaccharide, xylo-oligosaccharide；

The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo Fiber in mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme 70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 30 DEG C, adds the glucose of mixture quality 3%, 0.2% mixing Strain, 30 DEG C are cultivated 48 hours, and speed of agitator is 20r/min, culture to 50 hours Guangs of L- half of addition mixture quality 0.1% Propylhomoserin and 0.05% Radix Glycyrrhizae Ultramicro-powder, after fermentation ends be concentrated under reduced pressure, freeze-drying, low-temperature grinding to particle diameter for 0.3mm is Obtain dietary fiber；

The onion powder, preparation method thereof comprises the following steps：

(1) Shaking culture

(2) 5L fermentation tank cultures

(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is adjusted in self-dissolving tank by 1: 1.5 mass ratio Slurry, adjusts pH value 8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 50 DEG C of insulation self-dissolving 24h；Add mixing enzyme preparation, consumption It is the 1% of yeast paste quality；Stirring, enzymatic hydrolysis 24h.

(5) being spray-dried 140 DEG C of spray drying makes moisture less than 10%, obtains final product yeast hydrolyate.

A kind of preparation method of the finishing period feed of promotion laying hen Development of Digestive Organs is with embodiment 1.

Embodiment 4

10 parts of corn, 10 parts of barley, 10 parts of wheat, oat 15,12 parts of dregs of beans, 1 part of Cottonseed Meal, 2 parts of rice bran meal, wheat bran 2 parts, 2 parts of oat bran, 1 part of pomace, 1 part of citrus pulp, 1 part of treaster, 2 parts of corn bran, 2 parts of the oenothera biennis dregs of rice, 0.8 part of stone flour, 0.8 part of calcium monohydrogen phosphate, 0.5 part of salt, 0.08 part of threonine, 0.12 part of methionine, 0.15 part of lysine, 1.5 parts of vegetable oil is multiple Close 1 part of premix, 0.2 part of prebiotics, 0.05 part of zinc methionine, 0.05 part of Hydrolysable Tannins acid, 0.08 part of complex enzyme formulation, mould 0.1 part of endotoxin adsorbent, 0.04 part of degrading mold toxin enzyme, 0.1 part, dietary fiber .3.5 parts of yeast hydrolyate；

The prebiotics is isomalto-oligosaccharide；

The dietary fiber preparation method is with embodiment 1；

The onion powder, preparation method thereof is with embodiment 1；

The preparation method of the yeast hydrolyate is with embodiment 1；

Embodiment 5

30 parts of corn, 15 parts of oat, 15 parts of dregs of beans, 3 parts of Cottonseed Meal, 2 parts of peanut meal, 3 parts of rapeseed dregs, 7 parts of rice bran meal, swallow 8 parts of wheat bran, 8 parts of treaster, 5 parts of corn bran, 2 parts of the oenothera biennis dregs of rice, 1.5 parts of stone flour, 1.5 parts of calcium monohydrogen phosphate, 0.2 part of salt, Soviet Union 0.08 part of propylhomoserin, 0.12 part of methionine, 0.18 part of lysine, 1.5 parts of vegetable oil, 1 part of compound premix, 0.05 part of prebiotics, 0.01 part of zinc methionine, 0.2 part of Hydrolysable Tannins acid, 0.05 part of complex enzyme formulation, 0.1 part of mold toxin sorbent, mycotoxin 0.04 part of digestive enzyme, 0.1 part of yeast hydrolyate, 5 parts of dietary fiber；

The prebiotics is xylo-oligosaccharide；

The dietary fiber preparation method is with embodiment 2；

The saccharomycete is the conventional saccharomyces cerevisiae in this area；

The onion powder, preparation method thereof is with embodiment 2；

The preparation method of the yeast hydrolyate is with embodiment 2；

The saccharomycete is the conventional saccharomyces cerevisiae in this area；

In above-described embodiment 1-5

The compound premix is produced for Liaoning Hefeng Animal Husbandry Co., Ltd, Q/HF J02.03-2012, and the Liao Dynasty raises pre- Word (2013) 003021；

Complex enzyme formulation：Shenyang Fengmei Biotechnology Co., Ltd. produces, and production licence number adds (2011) 1986 for feeding, Authentication code：(adding) word (2011) 040008 is raised by the Liao Dynasty.

Test example

Influence by the tests below research present invention to laying hen finishing period Development of Digestive Organs and production performance

1 test material

1.1 test periods

Test in August in 2015 15 days --- it is on October 25th, 2015, whole 70 days, including pre-feeding period 7 days.

1.2 test materials

Experiment is implemented in Huan Ren Xinhua of Liaoning Province chicken house He Feng group laying hens proving ground.Randomly choose 43 ages in days sea blue brown Commodity egg 2520, experiment is allocated as 6 groups using single factor experiment design, test chicken, and every group sets 6 repetitions, and each repeats 70 Chicken；

Control group is common laying hen material.Test group feeds the finishing period feed of embodiment of the present invention 1-5 preparations respectively.

Feeding method：According to the conventional scale of feeding difference fed control group of this area and feed of the present invention；

1.3 feedings and managements are carried out according to chicken house daily management.This experiment is continued to 70 days, pre-feeding period 7 days.

1.4 testing indexs：Initial 43 age in days body weight, 105 age in days body weight；Tibia length (43 ages in days when initial, 105 days Age), calculate the body weight uniformity and the shin bone uniformity；Dead chicken number, calculates survival rate at the end of being bred as；Feed intake.

Carcass Index：Muscular stomach+glandular stomach weight, jejunum, duodenum, ileum, the weight and length of blind rectum；Heart, liver Dirty, kidney, pancreas and spleen weight；By each embodiment data averagely experimental group number evidence of the present invention, be shown in Table 5, table 6；

Table 5：Influence of the present invention to the age in days Development of Digestive Organs of laying hen 105

From table 5, to the end of term is raised, compared to control group, muscular stomach improves 9.3%, index and spleen index with glandular stomach index Improve 46%；Duodenum and small intestine weight have been respectively increased 33.6% and 22.1%, duodenum length and small intestinal length It has been respectively increased 33.4% and 28.6%.Digestive organs and immune organ are significantly better than control group.Fallopian tubal length and weight are all It is significantly improved, the development of ovary is had no in slaughtering process.

Table 6：Influence of the present invention to the age in days production performance of laying hen 105

Remarks：Colleague shoulder mark difference person significant difference (P ＜ 0.05)

Feed of the present invention is fed from 43 ages in days, to 105 ages in days, body weight is up to standard, better than control group；Survival rate is improved 5.7%, effect is significant.The shin uniformity long and the body weight uniformity are significantly improved.

By data above it may be concluded that a kind of finishing period feed for promoting laying hen Development of Digestive Organs of the present invention is promoting Enter the aspect effect is significant such as Development of Digestive Organs and Development of Immune Organs, and significantly improve survival rate and the body weight uniformity.

Claims

1. a kind of finishing period feed of promotion laying hen Development of Digestive Organs, is prepared by the raw material of following parts by weight：

Cereal 45-60 parts, dregs of beans 8-15 parts, 1-8 parts of miscellaneous dregs of rice class, bran 6-15 parts, 3-8 parts of pomace class, corn bran 2-5 parts, 2 parts of the oenothera biennis dregs of rice, stone flour 0.8-1.5 parts, calcium monohydrogen phosphate 0.8-1.5 parts, salt 0.2-0.5 parts, amino acid 0.35-0.43 parts, Vegetable oil 0.5-1.5 parts, 1 part of compound premix, prebiotics 0.05-0.2 parts, zinc methionine 0.01-0.05 parts, Hydrolysable Tannins acid 0.05-0.2 parts, complex enzyme formulation 0.05-0.08 parts, mold toxin sorbent 0.02-0.1 parts, degrading mold toxin enzyme 0.04- 0.2 part, yeast hydrolyate 0.1-0.5 parts, dietary fiber 1-5 parts；

Characterized in that, the dietary fiber is mixed by onion powder, barley fiber, common oats fibre, wheat embryo fiber, through ultrasound Extract, biology enzyme enzymolysis after fermentation is obtained；

The dietary fiber preparation method is comprised the following steps：By onion powder, barley fiber, common oats fibre, wheat embryo fiber In mass ratio 1:3:8:3 uniform mixing, add the water of 8 times of mixture quality, room temperature 300W, 40KHz condition ultrasonic extraction 20min, is 5.5 with newborn acid for adjusting pH value, adds the biology enzyme of mixture quality 0.2%, and 45min is digested in 50 DEG C, and go out enzyme；Enzyme 70 DEG C of liquid of solution, 18-20MPa homogeneous after material is cooled to 25-30 DEG C, adds the glucose of mixture quality 3-5%, 0.2- 0.5% mixed bacteria, 25-30 DEG C is cultivated 48-72 hours, and speed of agitator is 20-30r/min, and culture was added by 50-55 hours The Cys of mixture quality 0.005-0.1% and the Radix Glycyrrhizae Ultramicro-powder of 0.05-0.1%, be concentrated under reduced pressure after fermentation ends, Freeze-drying, low-temperature grinding are to particle diameter for 0.1-0.3mm obtains final product dietary fiber.

2. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that the Mixed Microbes Plant and contain following living bacteria count：Bacillus subtilis >=10,000,000,000/g, bacillus licheniformis >=100,000,000,000/g, saccharomycete >=100 Hundred million/g, lactic acid bacteria >=5,000,000,000/g.

3. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 2, it is characterised in that the saccharomycete It is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number is CGMCC No.12789.

4. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that the biology enzyme It is zytase, cellulase, laccase, pectase, tannase in mass ratio 4:9:2:4:1 uniform mixing.

5. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that the onion powder Preparation method comprises the following steps：

Fresh onion removes crust, cleaning, adds the water mashing of 12 times of onion weight, and the mixing of onion weight 0.03% is added afterwards Enzyme preparation is digested, and regulation pH value is 4.5, and 55 DEG C of temperature digests 3h, by enzymolysis liquid under the conditions of 55 DEG C, 300W, 80KHz Ultrasonic extraction 20min, 4-6 DEG C of placement 12h, vacuum concentration, freeze-drying, crushing obtain final product onion powder after filtering；

The mixing enzyme preparation is made up of the raw material of following parts by weight：Cellulase 2, protease 1, AMS 2, xylan Enzyme 0.5；

6. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that the yeast water The preparation method of thing is solved, is comprised the following steps：

(1) Shaking culture

The ring of inclined-plane barms one is taken, is accessed and is equipped with the 250mL shaking flasks of 30mL Shake flask mediums, 150rpm, 30 DEG C of culture 30h Obtain yeast starter liquid；

(2) 5L fermentation tank cultures

Yeast starter liquid is pressed into 10% mass percent inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation mediums, 30 DEG C, led to Tolerance 6L/min, tank pressure 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, ferment during to 30h, disposably add Plus the Cys of final concentration of 25mmol/L, total fermentation time is 50h, obtains zymotic fluid；

Fermentation medium (g/L)：(NH₄)₂SO₄10th, glucose 100, K₂HPO₄·3H₂O 8、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO₄ 0.1、MgSO₄·7H₂O 0.1,pH6.0；

(4) breaking yeast cellule membrane：Yeast paste and water obtained by step (3) is sized mixing in self-dissolving tank by 1: 1.5 mass ratio, is adjusted Whole pH value 6~8, is warmed to 80 DEG C, inactivates 40 minutes；Cool to 40 DEG C~50 DEG C insulation self-dissolving 24h；Add mixing enzyme preparation, Consumption is the 1%~1.5% of yeast paste quality；Stirring, enzymatic hydrolysis 10h~24h；

The mixing enzyme preparation is by protease, dextranase, mannonase cellulase, fire resistant alpha-diastase mixed in equal amounts Form；

7. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 6, it is characterised in that the saccharomycete It is preservation of bacteria strain saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, deposit number is CGMCC No.12789.

8. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that

The cereal is at least one of corn, barley, wheat, oat；

The miscellaneous dregs of rice class is at least one of Cottonseed Meal, peanut meal, rapeseed dregs；

The bran is at least one of rice bran meal, wheat bran, oat bran；

The pomace class is at least one of pomace, citrus pulp, treaster.

9. the finishing period feed of laying hen Development of Digestive Organs is promoted according to claim 1, it is characterised in that the amino acid Mass ratio be：Lysine：Threonine：Methionine=1:0.44-0.63:0.67-0.88.

10. the preparation method of any finishing period feeds for promoting laying hen Development of Digestive Organs of claim 1-9, including following Step：

Each component raw material accurately is weighed according to formula, corn, dregs of beans, barley, miscellaneous dregs of rice class, bran are pressed by 6.0mm sieves More solito egg feedstuff processing method is crushed, and complex enzyme formulation, prebiotics and compound premix is pre-mixed, then by weight Number is arrogant to small to sequentially add various raw materials, is well mixed, processing granulation, obtains final product the incubation for promoting laying hen Development of Digestive Organs Phase feed.