CN111728082A - Natural plant micro-ecological feed additive for fermented feed of laying hens and preparation method thereof - Google Patents
Natural plant micro-ecological feed additive for fermented feed of laying hens and preparation method thereof Download PDFInfo
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- CN111728082A CN111728082A CN202010373332.XA CN202010373332A CN111728082A CN 111728082 A CN111728082 A CN 111728082A CN 202010373332 A CN202010373332 A CN 202010373332A CN 111728082 A CN111728082 A CN 111728082A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a natural plant micro-ecological feed additive for fermented feed of laying hens and a preparation method thereof, belonging to the field of feed additives. The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product; the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax; the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei. In the additive prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens, all the live bacteria and metabolites thereof act together to improve the immunity and disease resistance of the laying hens, greatly reduce the death and culling rate, have no toxic or side effect and no drug residue, and also can improve the laying rate of the laying hens and improve the quality of eggs.
Description
Technical Field
The invention relates to a natural plant micro-ecological feed additive for fermented feed of laying hens and a preparation method thereof, belonging to the field of feed additives.
Background
The breeding of the laying hens has a plurality of problems at present, such as low and unstable laying rate, thin eggshells, light yolk color, unsuitable temperature and humidity of the environment or diseases, high death and culling rate caused by stress and the like, so that the breeding cost of the laying hens is increased invisibly. Blindly increasing the laying rate by increasing the feeding amount not only increases the cost, but also leads the chicken to be fat and fatty liver to appear too early, thus leading the death and culling rate to be increased; the disease control of the laying hens is not easy for culturists, and after the laying hens are started, when the laying hens have certain diseases and the medicines are necessary, some common medicines such as aminophylline, aureomycin, sulfonamides and the like can inhibit or damage the system of the laying hens.
Disclosure of Invention
The invention aims to provide a preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens, and the prepared additive can improve the laying rate of the laying hens, reduce the death and culling rate of the laying hens and has no toxic or side effect.
The invention also provides a natural plant micro-ecological feed additive for the fermented feed of the laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens.
In order to realize the aim, the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens adopts the technical scheme that:
a preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product; the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax; the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum and lactic acid bacteria.
The invention relates to a preparation method of a natural plant microecological feed additive for a fermented feed of laying hens, which takes a culture material and a traditional Chinese medicine composition as fermentation substrates, wherein the culture material provides nutrient substances required by the growth of microorganisms, the traditional Chinese medicine composition and the microorganisms have synergistic effect, the active ingredients of liquorice, astragalus and acanthopanax are improved by microbial transformation while providing cellulose carbon sources for the microorganisms, and polysaccharides, vitamins, amino acids and the like in the traditional Chinese medicine composition promote the growth and the propagation of clostridium butyricum, lactic acid bacteria, bacillus subtilis and bacillus licheniformis in the biotransformation process, and promote the generation of metabolic products such as cellulase, protease, amylase, butyric acid, lactic acid and the like. Meanwhile, the liquorice can effectively remove excessive free radicals generated by heat stress, enhance the antioxidation function of animal organisms, improve the feed intake and improve the stress state of animals; the astragalus has the function of immunoregulation, and improves the immunity and the stress state of animal organisms; the acanthopanax can stimulate the cell immunity and enhance the disease resistance of animals; the combination of the three components can enhance the immunity of animals, enhance the disease resistance, improve the feed intake and improve the stress state. Specific microorganisms are selected for closed fermentation, and the prepared additive contains active bacillus subtilis, bacillus licheniformis, clostridium butyricum, lactic acid bacteria, a small amount of saccharomyces cerevisiae, and also contains metabolic products such as amylase, protease, cellulase, lipase, lactic acid, butyric acid, amino acid, vitamin B, vitamin K, small peptide and the like generated by the additive; meanwhile, the traditional Chinese medicine is converted into small molecular components which can be directly absorbed by intestinal tracts of laying hens through microorganisms, so that the traditional Chinese medicine is easier to play a role. The clostridium butyricum resists the colonization of pathogenic bacteria through the same complex carbohydrate receptor on lipid and protein which compete with the pathogenic bacteria for the intestinal epithelium villus length, thereby improving the health level of the intestinal tract; the bacillus subtilis and the bacillus licheniformis can generate enzymes such as amylase, protease, cellulase, lipase and the like, improve the digestion capacity of small intestines, promote the digestion and absorption of food, and improve the utilization rate of feed, thereby improving the feed-egg ratio; meanwhile, bacillus subtilis and bacillus licheniformis can quickly consume oxygen to inhibit harmful bacteria such as escherichia coli and the like; amino acid, vitamin B, vitamin K, small peptide and the like generated by clostridium butyricum can provide richer and balanced nutrition for laying hens, and the generated butyric acid and lactic acid can reduce the pH value of intestinal tracts of the laying hens, inhibit the growth and reproduction of harmful bacteria such as escherichia coli and the like, are beneficial to the health of the intestinal tracts and reduce intestinal diseases such as diarrhea; the saccharomyces cerevisiae thalli can provide richer protein nutrition for the laying hens. The living bacteria and the metabolites thereof act together to improve the immunity and disease resistance of the laying hens, greatly reduce the death and culling rate, have no toxic or side effect and no drug residue, and can also improve the laying rate of the laying hens and the quality of eggs.
Preferably, the mass ratio of the liquorice, the astragalus and the acanthopanax is 25-30: 20-24: 10-15.
Preferably, the ratio of the total number of the bacterial colonies of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the clostridium butyricum and the lactic acid bacteria is (2.5-4.2) × 1011:(2.5～4.2)×1011:(0.06～1.2)×1011:(0.08～500)×108:(1.2～20)×1011More preferably, the ratio of the total number of colonies of Bacillus subtilis, Bacillus licheniformis, Saccharomyces cerevisiae, Clostridium butyricum, Lactobacillus acidophilus, enterococcus faecalis and Lactobacillus casei is (2.5-4.2) × 1011:(2.5～4.2)×1011:(0.06～1.2)×1011:(0.08～500)×108:(0.4～8)×1011:(0.4～4)×1011:(0.4～8)×1011It should be noted that the total number of bacterial colonies in the present invention is obtained by multiplying the effective viable count (CFU/mL or CFU/g) of the bacterial strain by the amount (volume or mass) of the bacterial strain, and the total number of bacterial colonies of the Bacillus subtilis used per 25-30 g of licorice is (2.5-4.2) × 1011CFU。
For example, the mixture is prepared using a process comprising the steps of: adding bacillus subtilis powder, bacillus licheniformis powder and saccharomyces cerevisiae powder into the mixed powder of the culture material, the liquorice, the astragalus and the acanthopanax, uniformly mixing, then adding the mixed bacterial liquid of clostridium butyricum and lactic acid bacteria, and uniformly mixing to obtain the compound fertilizer.
The compost, the liquorice, the astragalus and the acanthopanax mixed powder can be obtained by naturally airing and then crushing the compost, the liquorice, the astragalus and the acanthopanax, drying at 70-90 ℃ and then uniformly mixing.
In the preparation method of the mixture, the viable count of the bacillus subtilis powder is (1.0-1.2) × 1011CFU/g, the viable count of the bacillus licheniformis bacterial powder is (1.0-1.2) × 1011CFU/g, the viable count of the saccharomyces cerevisiae bacterial powder is (0.2-2.4) × 1011CFU/g。
In the preparation method of the mixture, the mixed bacterial liquid is mixed bacterial liquid of clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei, and the effective viable count of the clostridium butyricum in the mixed bacterial liquid is (0.001-5.0) × 108CFU/mL, effective viable count of Lactobacillus acidophilus of 0.5-8.0 × 109CFU/mL, the effective viable count of enterococcus faecalis is (0.5-4.0) × 109CFU/mL, effective viable count of Lactobacillus casei is (0.5-8.0) × 109CFU/mL。
In the preparation method of the mixture, 80-100 mL of mixed bacterial liquid is adopted for every 25-30 g of liquorice.
In addition, the mixtures can also be prepared by the methods of method a, method B, method C or method D. The method A comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, Saccharomyces cerevisiae powder, Clostridium butyricum powder and lactobacillus powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding water, and stirring. The method B comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, Saccharomyces cerevisiae powder and lactobacillus powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding Clostridium butyricum liquid, and mixing. Method C comprises the steps of: adding bacillus subtilis powder, bacillus licheniformis powder, saccharomyces cerevisiae powder and clostridium butyricum powder into the mixed powder of the culture material, the liquorice, the astragalus and the acanthopanax, uniformly mixing, then adding lactobacillus liquid, and uniformly mixing to obtain the compound fertilizer, wherein the method D comprises the following steps: adding Bacillus subtilis powder, Bacillus licheniformis powder, and Saccharomyces cerevisiae powder into the mixed powder of culture medium, Glycyrrhrizae radix, radix astragali and radix Acanthopanacis Senticosi, mixing, adding Clostridium butyricum liquid and lactobacillus liquid, and mixing.
Preferably, in the method A, 80-100 mL of water is adopted for every 25-30 g of liquorice. In the method B, 80-100 mL of clostridium butyricum bacterial liquid is correspondingly adopted for every 25-30 g of liquorice. In the method C, 80-100 mL of lactobacillus bacterial liquid is correspondingly adopted for every 25-30 g of liquorice. In the method D, 80-100 mL of clostridium butyricum bacterial liquid and 0.5-2 mL of lactobacillus bacterial liquid are correspondingly adopted for every 25-30 g of liquorice.
Preferably, the culture material is selected from one or any combination of bran, corn, rice chaff and gunite corn chaff.
Preferably, the mass ratio of the culture material to the liquorice is 36-40: 25-30.
Preferably, the fermentation temperature is 33-37 ℃ and the fermentation time is 5-7 days.
Preferably, the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens further comprises drying the product obtained after fermentation. Further preferably, the temperature of the drying treatment is 50 to 70 ℃.
The product obtained by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens has the effective viable count that the bacillus subtilis is more than or equal to 1.0 × 109CFU/g, more than or equal to 1.0 × 10 of bacillus licheniformis9CFU/g, Saccharomyces cerevisiae ≥ 1.0 × 102CFU/g, Clostridium butyricum is more than or equal to 1.0 × 107CFU/g, lactic acid bacteria not less than 1.0 × 109CFU/g。
The invention relates to a natural plant micro-ecological feed additive for fermentation feed of laying hens, which adopts the technical scheme that:
the natural plant micro-ecological feed additive for the fermented feed of the laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens.
The natural plant micro-ecological feed additive for the fermented feed of the laying hens has no toxic or side effect and no drug residue, can improve the laying rate of the laying hens, improve the egg quality and reduce the death rate of the laying hens, and the effective viable count of the natural plant micro-ecological feed additive for the fermented feed of the laying hens can reach that the number of bacillus subtilis is more than or equal to 1.0 × 109CFU/g, more than or equal to 1.0 × 10 of bacillus licheniformis9CFU/g, Saccharomyces cerevisiae ≥ 1.0 × 102CFU/g, Clostridium butyricum is more than or equal to 1.0 × 108CFU/g, lactic acid bacteria not less than 1.0 × 109CFU/g. Beneficial microorganisms in the additive can regulate intestinal microecological balance, enhance immunity of laying hens, inhibit growth and reproduction of harmful microorganisms, and reduce risk and probability of diseases of chicken flocks, such as short-chain fatty acids such as butyric acid and lactic acid produced by clostridium butyricum and lactic acid bacteria, can stimulate intestinal peristalsis and improve intestinal microenvironment; the hydrolase such as cellulase, amylase, protease and the like generated by the bacillus licheniformis and the bacillus subtilis can promote the digestion and absorption of nutrient substances; more importantly, the functions of the probiotics can not generate toxic and harmful substances and side effects, can not generate drug residues, accords with advocated non-resistance and resistance reduction, provides health protection and navigation for animals and plants, and provides green and nutritional food for human beings.
Detailed Description
The present invention will be further described with reference to the following embodiments.
In examples 1 to 3, when the mixed bacterial liquid was prepared, the clostridium butyricum strain deposited number in the clostridium butyricum fermentation liquid was cic 10390, and the strain in the lactic acid bacteria liquid used was JBHBS1Lactobacillus acidophilus strain, JBHBSC1Combination of enterococcus faecalis strains and JBHBGL Lactobacillus casei strains, JBHBS1The Lactobacillus acidophilus strain has a preservation number of ACCC05489, JBHBSC1The enterococcus faecalis strain is deposited with the number CICC23658, JBHBGL Lactobacillus caseiThe strain preservation number is CICC 20995; the volume of the adopted lactobacillus liquid is 5 percent of that of the clostridium butyricum fermentation centrifugate; the carbohydrate carbon source is the combination of glucose and molasses, the mass of the glucose is 2.0% of that of the clostridium butyricum fermentation centrifugate, and the mass of the molasses is 1.5% of that of the clostridium butyricum fermentation centrifugate; the nitrogen source is corn steep liquor, and the mass of the corn steep liquor is 1.0 percent of that of the clostridium butyricum fermentation centrifugate; the inorganic salt is MgSO4、MnSO4、K2HPO4And sodium acetate, MgSO4The mass of the clostridium butyricum fermentation centrifugate is 0.025 percent of the mass of the clostridium butyricum fermentation centrifugate, and the mass of the clostridium butyricum fermentation centrifugate is MnSO4The mass of (a) is 0.015% of that of the clostridium butyricum fermentation centrifugate, and K2HPO4The mass of the sodium acetate is 0.6 percent of the mass of the clostridium butyricum fermentation centrifugate.
Example of preparation method of natural plant micro-ecological feed additive for fermented feed of laying hens
Example 1
The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 40 parts by weight of bran, 30 parts by weight of liquorice, 20 parts by weight of astragalus and 10 parts by weight of acanthopanax, respectively crushing, drying in an oven at 70 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 3.5 parts by weight of effective viable bacteria with the number of 1.0 × 10 into the mixed powder obtained in the step 1)11CFU/g bacillus subtilis powder, 3.5 weight parts of effective viable count of 1.2 × 1011CFU/g Bacillus licheniformis powder, 0.5 weight part of effective viable count of 2.0 × 1010Uniformly stirring the CFU/g saccharomyces cerevisiae powder to obtain a mixture A;
3) adding the mixed bacterial liquid into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
the adding amount of the mixed bacterial liquid is determined according to the proportion that every 30g of liquorice correspondingly adopts 100mL of mixed bacterial liquid;
the adopted mixed bacterial liquid is dicedThe mixed bacterial liquid of clostridium acidocaldarium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei, and the effective viable count of the clostridium butyricum in the mixed bacterial liquid is 7.0 × 107CFU/mL, effective viable count of Lactobacillus acidophilus 3.8 × 109CFU/mL, enterococcus faecalis content 3.2 × 109CFU/mL, effective viable count of Lactobacillus casei 4.7 × 109CFU/mL, adding a carbohydrate carbon source, a nitrogen source and inorganic salt into clostridium butyricum fermentation centrifugate, then inoculating a lactobacillus strain, and performing closed fermentation at 37 ℃ for 48 hours to obtain a mixed bacterial liquid;
wherein the content of Clostridium butyricum in the fermentation centrifugate of Clostridium butyricum is 2.6 × 106CFU/mL; JBHBS in lactobacillus bacterial liquid adopted in lactobacillus strain inoculation1Is 1.0 × 109CFU/mL，JBHBSC1Is 7.0 × 108The content of CFU/mL and JBHBGL is 1.0 × 109CFU/mL, wherein the volume of the lactobacillus liquid is 5% of the volume of the clostridium butyricum fermentation centrifugate;
4) compacting the mixture B obtained in the step 3), and fermenting in a closed manner at 33 ℃ for 7 days to obtain a mixture C;
5) and then drying the mixture C obtained in the step 4) at 50 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for fermentation of laying hens prepared by the embodiment contains bacillus subtilis 2.7 × 109CFU/g, Bacillus licheniformis 2.6 × 109CFU/g, Saccharomyces cerevisiae 1.4 × 102CFU/g, Clostridium butyricum 3.2 × 108CFU/g, lactic acid bacteria (including Lactobacillus acidophilus 2.6 × 10)9CFU/g, enterococcus faecalis 1.4 × 109CFU/g and Lactobacillus casei 2.0 × 109CFU/g)6.0×109CFU/g。
Example 2:
the preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 38 parts by weight of bran, 28 parts by weight of liquorice, 22 parts by weight of astragalus and 12 parts by weight of acanthopanax, respectively crushing, drying in an oven at 80 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 3 parts by weight of effective viable bacteria with the number of 1.1 × 10 into the mixed powder obtained in the step 1)11CFU/g bacillus subtilis powder, 3 weight parts of effective viable count of 1.1 × 1011CFU/g Bacillus licheniformis powder, 0.4 weight part of effective viable count of 2.1 × 1010Uniformly stirring the CFU/g saccharomyces cerevisiae powder to obtain a mixture A;
3) adding the mixed bacterial liquid into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
the adding amount of the mixed bacterial liquid is determined according to the proportion that 90mL of mixed bacterial liquid is adopted for every 28g of liquorice;
the adopted mixed bacterial liquid is the mixed bacterial liquid of clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei, and the effective viable count of the clostridium butyricum in the mixed bacterial liquid is 2.2 × 107CFU/mL, effective viable count of Lactobacillus acidophilus is 5.2 × 109CFU/mL, effective viable count of enterococcus faecalis 4.3 × 109CFU/mL, effective viable count of Lactobacillus casei 3.0 × 109CFU/mL, adding a carbohydrate carbon source, a nitrogen source and inorganic salt into clostridium butyricum fermentation centrifugate, then inoculating a lactobacillus strain, and performing closed fermentation at 37 ℃ for 48 hours to obtain a mixed bacterial liquid;
wherein the content of Clostridium butyricum in the fermentation centrifugate of Clostridium butyricum is 3.8 × 106CFU/mL; JBHBS in lactobacillus bacterial liquid adopted in lactobacillus strain inoculation1Is 9 × 108CFU/mL，JBHBSC1Is in an amount of 7.6 × 108The content of CFU/mL and JBHBGL is 9.3 × 108CFU/mL, wherein the volume of the lactobacillus liquid is 5% of the volume of the clostridium butyricum fermentation centrifugate;
4) compacting the mixture B obtained in the step 3), and fermenting in a closed manner at 35 ℃ for 6 days to obtain a mixture C;
5) and then drying the mixture C obtained in the step 4) at 60 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for the fermented feed of the laying hens prepared by the embodiment contains bacillus subtilis 2.9 × 109CFU/g, Bacillus licheniformis 2.7 × 109CFU/g, Saccharomyces cerevisiae 1.5×102CFU/g, Clostridium butyricum 4.4 × 108CFU/g, lactic acid bacteria (including Lactobacillus acidophilus 2.5 × 10)9CFU/g, enterococcus faecalis 1.3 × 109CFU/g and Lactobacillus casei 2.0 × 109CFU/g)5.8×109CFU/g。
Example 3
The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 36 parts by weight of bran, 25 parts by weight of liquorice, 24 parts by weight of astragalus and 15 parts by weight of acanthopanax, respectively crushing, drying in an oven at 90 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 2.5 parts by weight of composite bacterial powder into the mixed powder obtained in the step 1), wherein the effective viable count is 1.2 × 1011CFU/g bacillus subtilis powder, 2.5 weight parts of effective viable count of 1.0 × 1011CFU/g Bacillus licheniformis powder, 0.3 weight part of effective viable count of 2.2 × 1010Uniformly stirring the CFU/g saccharomyces cerevisiae powder to obtain a mixture A;
3) adding the mixed bacterial liquid into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
the adding amount of the mixed bacterial liquid is determined according to the proportion that 80mL of mixed bacterial liquid is adopted for every 25g of liquorice;
the adopted mixed bacterial liquid is the mixed bacterial liquid of clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei, and the effective viable count of the clostridium butyricum in the mixed bacterial liquid is 6.0 × 107CFU/mL, effective viable count of Lactobacillus acidophilus is 5.9 × 109CFU/mL, effective viable count of enterococcus faecalis 5.3 × 109CFU/mL, effective viable count of Lactobacillus casei 6.0 × 109CFU/mL, adding a carbohydrate carbon source, a nitrogen source and inorganic salt into clostridium butyricum fermentation centrifugate, then inoculating a lactobacillus strain, and performing closed fermentation at 37 ℃ for 48 hours to obtain a mixed bacterial liquid;
wherein the content of Clostridium butyricum in the fermentation centrifugate of Clostridium butyricum is 4.2 × 106CFU/mL; inoculation of lactic acid bacteria strainsJBHBS in the lactobacillus bacterial liquid adopted1Is 1.2 × 109CFU/mL，JBHBSC1Is 9.7 × 108The content of CFU/mL and JBHBGL is 1.1 × 109CFU/mL, wherein the volume of the lactobacillus liquid is 5% of the volume of the clostridium butyricum fermentation centrifugate;
4) compacting the mixture B obtained in the step 3), and then fermenting in a closed manner at 37 ℃ for 5 days to obtain a mixture C;
5) drying the mixture C obtained in the step 4) at 70 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for the fermented feed of the laying hens, which is prepared by the embodiment, contains bacillus subtilis 2.8 × 109CFU/g, Bacillus licheniformis 2.8 × 109CFU/g, Saccharomyces cerevisiae 1.6 × 102CFU/g, Clostridium butyricum 5.8 × 108CFU/g, lactic acid bacteria (including Lactobacillus acidophilus 2.7 × 10)9CFU/g, enterococcus faecalis 1.1 × 109CFU/g and Lactobacillus casei 2.4 × 109CFU/g)6.2×109CFU/g。
Example 4
The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 40 parts by weight of bran, 30 parts by weight of liquorice, 20 parts by weight of astragalus and 10 parts by weight of acanthopanax, respectively crushing, drying in an oven at 70 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 3.5 parts by weight of effective viable bacteria with the number of 1.0 × 10 into the mixed powder obtained in the step 1)11CFU/g bacillus subtilis powder, 3.5 weight parts of effective viable count of 1.2 × 1011CFU/g Bacillus licheniformis powder, 0.5 weight part of effective viable count of 2.0 × 1010CFU/g Saccharomyces cerevisiae powder, 1.4 weight parts of effective viable count of 5.0 × 109CFU/g clostridium butyricum powder, 0.12 weight parts of effective viable count of 1.0 × 1011Uniformly stirring the CFU/g lactobacillus powder to obtain a mixture A;
3) adding purified water into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
the adding amount of the purified water is determined according to the proportion that every 30g of liquorice adopts 100mL of purified water;
4) compacting the mixture B obtained in the step 2), and fermenting in a closed manner at 33 ℃ for 7 days to obtain a mixture C;
5) and then drying the mixture C obtained in the step 4) at 50 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for fermentation of laying hens prepared by the embodiment contains bacillus subtilis 2.6 × 109CFU/g, Bacillus licheniformis 2.5 × 109CFU/g, Saccharomyces cerevisiae 1.3 × 102CFU/g, Clostridium butyricum 8.3 × 107CFU/g, lactic acid bacteria 2.5 × 109CFU/g。
Example 5
The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 40 parts by weight of bran, 30 parts by weight of liquorice, 20 parts by weight of astragalus and 10 parts by weight of acanthopanax, respectively crushing, drying in an oven at 70 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 3.5 parts by weight of effective viable bacteria with the number of 1.0 × 10 into the mixed powder obtained in the step 1)11CFU/g bacillus subtilis powder, 3.5 weight parts of effective viable count of 1.2 × 1011CFU/g Bacillus licheniformis powder, 0.5 weight part of effective viable count of 2.0 × 1010CFU/g Saccharomyces cerevisiae powder, 0.12 weight parts of effective viable count of 1.0 × 1011Uniformly stirring the CFU/g lactobacillus powder to obtain a mixture A;
3) adding clostridium butyricum fermentation centrifugate into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
clostridium butyricum fermentation centrifugate (the effective viable count of clostridium butyricum is 7.0 × 10)7CFU/mL) is determined according to the proportion of 100mL clostridium butyricum fermentation centrifugate adopted for every 30g of liquorice;
4) compacting the mixture B obtained in the step 2), and fermenting in a closed manner at 33 ℃ for 7 days to obtain a mixture C;
5) and then drying the mixture C obtained in the step 4) at 50 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for fermentation of laying hens prepared by the embodiment contains bacillus subtilis 2.5 × 109CFU/g, Bacillus licheniformis 2.4 × 109CFU/g, Saccharomyces cerevisiae 1.3 × 102CFU/g, Clostridium butyricum 1.9 × 108CFU/g, lactic acid bacteria 4.2 × 109CFU/g。
Example 6
The preparation method of the natural plant micro-ecological feed additive for the fermented feed of the laying hens comprises the following steps:
1) naturally airing 40 parts by weight of bran, 30 parts by weight of liquorice, 20 parts by weight of astragalus and 10 parts by weight of acanthopanax, respectively crushing, drying in an oven at 70 ℃ to obtain powder, and uniformly mixing and stirring the dried powder to obtain mixed powder;
2) adding 3.5 parts by weight of effective viable bacteria with the number of 1.0 × 10 into the mixed powder obtained in the step 1)11CFU/g bacillus subtilis powder, 3.5 weight parts of effective viable count of 1.2 × 1011CFU/g Bacillus licheniformis powder, 0.5 weight part of effective viable count of 2.0 × 1010Uniformly stirring the CFU/g saccharomyces cerevisiae powder to obtain a mixture A;
3) adding clostridium butyricum fermentation centrifugate and lactobacillus fermentation liquor into the mixture A obtained in the step 2), and uniformly stirring to obtain a mixture B;
clostridium butyricum fermentation centrifugate (the effective viable count of clostridium butyricum is 7 × 10)7CFU/mL) is determined according to the proportion of 100mL clostridium butyricum fermentation centrifugate per 30g liquorice, and lactobacillus fermentation liquid (the effective viable count of lactobacillus is 1.2 × 10)10CFU/mL) is determined according to the proportion of 1mL clostridium butyricum fermentation centrifugate adopted for every 30g of liquorice;
4) compacting the mixture B obtained in the step 2), and fermenting in a closed manner at 33 ℃ for 7 days to obtain a mixture C;
5) and then drying the mixture C obtained in the step 4) at 50 ℃ to obtain the composite material.
The natural plant micro-ecological feed additive for fermentation of laying hens prepared by the embodiment contains bacillus subtilis 2.4 × 109CFU/g, Bacillus licheniformis 2.6 × 109CFU/g, Saccharomyces cerevisiae 1.2 × 102CFU/g, Clostridium butyricum 2.7 × 108CFU/g, lactic acid bacteria 5.1 × 109CFU/g。
The natural plant micro-ecological feed additive for the fermented feed of the laying hens prepared in the above embodiments 1 to 6 is sent to a third party detection mechanism for detection, and meets the following regulations:
1) the raw materials and strains of the product meet the requirements of feed raw material catalogues and feed additive variety catalogues (2013).
2) The product is powder or granule, has consistent color, no odor, peculiar smell and agglomeration, and has special fermentation smell.
3) The product was all passed through a 40 mesh screen.
4) The water content in the product is not more than 10%.
5) The product meets the sanitation indexes in table 1.
TABLE 1 sanitation index
| Total arsenic in mg/kg | Lead, mg/kg | Aflatoxin B1，μg/kg | Salmonella |
| ≤2.0 | ≤5.0 | ≤10.0 | Cannot be detected |
Examples of natural plant micro-ecological feed additives for fermented feed for laying hens
Example 7
The natural plant micro-ecological feed additive for the fermented feed of the laying hens in the embodiment is prepared by the preparation method of the embodiments 1 to 6, and is not described again.
Examples of the experiments
The experimental example is an animal clinical test.
1) In a Taihe county Bingchang Zhen farm, 5500 Hailan brown chickens are 196 days old, 1kg of the natural plant micro-ecological feed additive for fermentation feeding of laying hens prepared in example 1 is added to each ton of complete feed by using local corns, compared with the prior feed additive-free feed, the amount of excrement is obviously reduced, the molding is better than that before, the laying rate reaches 95%, the daily basically non-damaged chickens have no damage, the net weight of 360 eggs is 22.5kg, and the feed-egg ratio is 2.0: 1.
2) According to the complete feed per ton, 1kg of the natural plant microecological feed additive for the fermented feed of the laying hens prepared in the example 2, 27t of food intake per month, 107.78g of food intake per day, 96% of laying rate, 22.5kg of net weight of 360 eggs and 1.85:1 of feed-egg ratio are added to a farm of Taihe county leather strips town, 8350 eggs are kept in stock and are 270 days old.
3) Feeding helan brown chicken under the condition of caging in an Anyang Maqian first breeding farm, selecting two adjacent cages in one chicken house, manually feeding 96 chickens and independently collecting eggs, taking a week of data before using the microecological feed additive as basic data, and then adding 1kg of the natural plant microecological feed additive for fermentation feeding of laying hens, which is prepared in the embodiment 3, into each ton of complete feed for an experiment, wherein the experiment period is 20 days and 7 months, the weather is very hot, and the basic data and the experiment period data are as follows:
TABLE 2 basic data
TABLE 3 run length data
The data of animal clinical experiments 1) to 3) show that the laying rate is over 94 percent, is obviously higher than that of the common laying hen breeding, and the death and culling rate is obviously reduced. Particularly, the data of the animal clinical experiment 3) show that the laying rate of the experimental group and the control group is obviously improved under the condition of basically the same feed intake, the feed-egg ratio is also reduced, the death rate is greatly reduced, the surface of the eggshell of the experimental group added with the mixed feed additive is smoother, the eggshell is not easy to break, the color of the yolk is obviously yellow compared with that of the control group, the yolk can be lifted for nearly 1 minute, and the control group can only be lifted for less than 30 seconds.
Claims (10)
1. A preparation method of a natural plant micro-ecological feed additive for fermented feed of laying hens is characterized by comprising the following steps: the method comprises the following steps: fermenting the mixture of microorganism, culture material and Chinese medicinal composition under sealed condition, and drying to obtain the final product;
the traditional Chinese medicine composition comprises liquorice, astragalus and acanthopanax;
the microorganism comprises bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum and lactic acid bacteria.
2. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the mass ratio of the liquorice, the astragalus and the acanthopanax is 25-30: 20-24: 10-15.
3. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1 or 2, wherein: the lactic acid bacteria include Lactobacillus acidophilus, enterococcus faecalis and Lactobacillus casei.
4. The preparation method of the natural plant micro-ecological feed additive for the fermented feed of laying hens according to claim 3The method is characterized in that the ratio of the total number of bacterial colonies of bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, clostridium butyricum, lactobacillus acidophilus, enterococcus faecalis and lactobacillus casei is (2.5-4.2) × 1011:(2.5～4.2)×1011:(0.06～1.2)×1011:(0.08～500)×108:(0.4～8)×1011:(0.4～4)×1011:(0.4～8)×1011。
5. The method for preparing a natural plant micro-ecological feed additive for a fermented feed of laying hens according to claim 1 or 2, wherein the total number of bacterial colonies of the bacillus subtilis adopted for every 25-30 g of liquorice is (2.5-4.2) × 1011CFU。
6. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the culture material is selected from one or any combination of bran, corn, rice chaff and gunite corn husk.
7. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the mass ratio of the culture material to the liquorice is 36-40: 25-30.
8. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1, wherein the method comprises the following steps: the fermentation temperature is 33-37 ℃, and the fermentation time is 5-7 days.
9. The method for preparing a natural plant micro-ecological feed additive for fermented feed of laying hens according to claim 1 or 8, wherein the natural plant micro-ecological feed additive comprises: the drying temperature is 50-70 ℃.
10. A natural plant micro-ecological feed additive for fermented feed for laying hens, which is prepared by the preparation method of the natural plant micro-ecological feed additive for fermented feed for laying hens according to claim 1.
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Cited By (4)
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| CN113508869A (en) * | 2021-08-16 | 2021-10-19 | 河南福佑侬生物科技有限公司 | Green fermented traditional Chinese medicine feed additive formula for livestock and poultry |
| CN114391603A (en) * | 2022-01-28 | 2022-04-26 | 河南省科学院生物研究所有限责任公司 | Feeding traditional Chinese medicine microecological preparation and preparation method thereof |
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| CN113508869A (en) * | 2021-08-16 | 2021-10-19 | 河南福佑侬生物科技有限公司 | Green fermented traditional Chinese medicine feed additive formula for livestock and poultry |
| CN114391603A (en) * | 2022-01-28 | 2022-04-26 | 河南省科学院生物研究所有限责任公司 | Feeding traditional Chinese medicine microecological preparation and preparation method thereof |
| CN115895982A (en) * | 2023-02-06 | 2023-04-04 | 大连理工大学 | Arsenic-resistant fermentation hydrogen-producing bacterium, screening and application |
| CN115895982B (en) * | 2023-02-06 | 2024-04-12 | 大连理工大学 | Arsenic-resistant fermentation hydrogen-producing bacterium, screening and application |
| CN116875483A (en) * | 2023-05-30 | 2023-10-13 | 大理州大台农台标饲料有限公司 | Composite microbial agent, fermented feed and preparation method thereof |
| CN116875483B (en) * | 2023-05-30 | 2024-04-09 | 大理州大台农台标饲料有限公司 | Composite microbial agent, fermented feed and preparation method thereof |
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