CN115895982B - Arsenic-resistant fermentation hydrogen-producing bacterium, screening and application - Google Patents
Arsenic-resistant fermentation hydrogen-producing bacterium, screening and application Download PDFInfo
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- 229910052785 arsenic Inorganic materials 0.000 title claims abstract description 59
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 238000000855 fermentation Methods 0.000 title abstract description 38
- 230000004151 fermentation Effects 0.000 title abstract description 34
- 241000894006 Bacteria Species 0.000 title abstract description 17
- 238000012216 screening Methods 0.000 title abstract description 14
- 239000001257 hydrogen Substances 0.000 title abstract description 9
- 229910052739 hydrogen Inorganic materials 0.000 title abstract description 9
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title abstract description 8
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 23
- 230000000813 microbial effect Effects 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 238000005067 remediation Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 25
- 230000001580 bacterial effect Effects 0.000 abstract description 15
- 239000001963 growth medium Substances 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 13
- 241000193403 Clostridium Species 0.000 abstract description 8
- 239000013049 sediment Substances 0.000 abstract description 8
- 239000012137 tryptone Substances 0.000 abstract description 8
- 239000011248 coating agent Substances 0.000 abstract description 7
- 238000000576 coating method Methods 0.000 abstract description 7
- 244000005700 microbiome Species 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract description 5
- 238000007865 diluting Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 238000003794 Gram staining Methods 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 16
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000003213 activating effect Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- 240000009087 Crescentia cujete Species 0.000 description 2
- 235000005983 Crescentia cujete Nutrition 0.000 description 2
- 235000009797 Lagenaria vulgaris Nutrition 0.000 description 2
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- MHUWZNTUIIFHAS-XPWSMXQVSA-N 9-octadecenoic acid 1-[(phosphonoxy)methyl]-1,2-ethanediyl ester Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(COP(O)(O)=O)OC(=O)CCCCCCC\C=C\CCCCCCCC MHUWZNTUIIFHAS-XPWSMXQVSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 231100000693 bioaccumulation Toxicity 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- -1 electron donors Inorganic materials 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229940047047 sodium arsenate Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of environmental microorganism screening, and discloses arsenic-resistant fermentation hydrogen-producing bacteria, screening and application. Taking a collected high-arsenic sediment sample, and adding the sample into an anaerobic glove box 5+ Placing in an anaerobic fermentation culture medium, shaking in a shaking table at 35 ℃ for dark culture, enriching and passaging, diluting bacterial liquid obtained by shaking culture by using a sterile anaerobic tryptone solution, coating the diluted bacterial liquid on a clostridium multiplication culture medium, and placing in an anaerobic biochemical incubator for light-proof culture; and (3) picking single colonies from the grown colonies, inoculating the single colonies into a liquid fermentation culture medium, placing the liquid fermentation culture medium in a shaking table for shake culture, and repeating the processes of coating and picking bacteria for three times to obtain the arsenic-resistant pure strain. The strain can be used for preparing a strain containing 200mg/L As 3+ The arsenic-resistant strain survives in a liquid culture medium, has better arsenic resistance, is positive in gram staining experiments, and is obtained through 16s rDNA sequence identification, thus the arsenic-resistant strain is typical zymophyte clostridium butyricum and provides strain resources for repairing arsenic-polluted environments by microorganisms.
Description
Technical Field
The invention belongs to the technical field of environmental microorganism screening, and particularly relates to screening of arsenic-resistant fermentation bacteria and fermentation application of the arsenic-resistant fermentation bacteria in repairing an arsenic-polluted environment.
Background
Industrial activities such as metallurgy and mining continuously discharge heavy metal arsenic into natural environment, and arsenic pollution prevention, treatment and repair problems are increasingly urgent due to high toxicity and bioaccumulation of arsenic. Arsenic and compounds thereof in natural environment are finally enriched into anaerobic environments such as soil, sediment and the like through migration and transformation, and anaerobic fermentation under anaerobic conditions is the most important process, so that energy sources such as hydrogen, electron donors, carbon sources and the like are provided for subsequent sulfate reduction and sulfate reduction bacteria biological ore-forming processes.
In conclusion, the discovery of the arsenic-resistant fermentation hydrogen-producing bacteria has important significance in the aspect of treating the arsenic pollution environment by the existing microorganisms.
Disclosure of Invention
The invention aims to screen out a strain of zymocyte with arsenic-resistant property, and provides application for repairing arsenic-polluted environment.
In order to achieve the application purpose, the technical scheme of the invention comprises the following steps:
an arsenic-resistant zymocyte (Clostridium butyricum) which has been preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 26434 in the month 1 of 2023.
Use of the above arsenic-tolerant fermenting bacterium having 200mg/L As 3+ Resistance, for the microbial remediation of arsenic-contaminated environments.
The screening method of the arsenic-resistant zymobacteria comprises the following steps:
firstly, taking an acquired high-arsenic sediment sample, adding the high-arsenic sediment sample into a pentavalent arsenic fermentation medium, and placing the high-arsenic sediment sample in a shaking table at 35 ℃ for shaking and light-shielding cultivation;
step two, the arsenic-resistant fermentation bacterial liquid after shaking culture is passaged into a pentavalent arsenic fermentation culture medium, and is placed in a shaking table for shaking and light-shielding culture for 20 hours at 35 ℃, and the step three is repeated to obtain the pentavalent arsenic fermentation culture medium enriched bacterial liquid;
thirdly, diluting the enriched bacterial liquid with an anaerobic sterile tryptone solution with the mass volume ratio of 0.1%, coating the diluted bacterial liquid onto a semi-solid clostridium multiplication culture medium, and placing the semi-solid clostridium multiplication culture medium in an anaerobic biological incubator at the temperature of 35 ℃ for constant temperature and light-shielding culture;
step four, picking single colony into a liquid fermentation culture medium, and placing the single colony into a shaking table at 35 ℃ for shaking and light-shielding culture;
fifthly, diluting the bacterial liquid obtained in the fourth step with an anaerobic sterile tryptone solution with the mass volume ratio of 0.1%, coating the bacterial liquid on a clostridium multiplication semi-solid medium, and placing the clostridium multiplication semi-solid medium in an anaerobic biological incubator at 35 ℃ for constant temperature and light-proof cultivation;
and sixthly, repeating the fourth step and the fifth step for three times to obtain the pure strain.
Further, in the third step, the bacterial liquid is diluted 10 with an anaerobic and aseptic tryptone solution with the mass-volume ratio of 0.1 percent 2 -10 8 Multiple of 10 times 5 、10 6 、10 7 、10 8 The bacterial liquid is coated on a clostridium multiplication semisolid culture with the diameter of 9cm, the volume of the coated bacterial liquid is 100 mu L, and the clostridium multiplication semisolid culture is placed in an anaerobic organism incubator at 35 ℃ for culture for 24-48 hours.
In the fourth step, the picked single bacterial colony is inoculated in a penicillin bottle containing a liquid fermentation culture medium in an anaerobic box, the inoculated sealed penicillin bottle is placed in a constant-temperature oscillating table at 35 ℃ at the rotating speed of 100r/min, and the culture time is 24 hours.
Further, in the first step, 0.14g of high-arsenic sediment sample is taken in a sterile anaerobic glove box, added into a penicillin bottle containing a sterile anaerobic liquid fermentation medium, and placed in a shaking table at 35 ℃ for 100r/min for shaking and light-shielding cultivation.
Further, in the first step and the second step, the components in the pentavalent arsenic fermentation medium: 10.0g of glucose, 1.0g of tryptone, 2.0g of yeast extract, 4.0g of sodium chloride, 0.1g of magnesium chloride, 0.5g of L-cysteine, 1.5g of dipotassium hydrogen phosphate, 10.0g of sodium bicarbonate, 0.5mg of resazurin, 166.5mg of sodium arsenate and 1L, pH 6.80.80-7.20 of ultrapure water.
Further, in the third step, the components in the clostridium proliferation semi-solid medium: 10.0g of beef powder, 10.0g of tryptone, 3.0g of yeast powder, 1.0g of soluble starch, 5.0g of sodium chloride, 3.0g of sodium acetate, 0.5g of L-cysteine, 1.0mg of resazurin, 5.0g of glucose, 8.0g of agar and 1. 1L, pH 6.60.60-7.00 of ultrapure water.
Further, in the fourth step, the components in the liquid fermentation medium: 10.0g of glucose, 1.0g of tryptone, 2.0g of yeast extract, 4.0g of sodium chloride, 0.1g of magnesium chloride, 0.5g of L-cysteine, 1.5g of dipotassium hydrogen phosphate, 10.0g of sodium bicarbonate, 0.5mg of resazurin and 1. 1L, pH 6.80.80-7.20 of ultrapure water.
The invention aims to provide a novel microorganism repairing method for treating an arsenic-polluted environment, wherein the method for treating the environmental arsenic pollution uses the screening method of arsenic-resistant bacteria.
The invention has the beneficial effects that: the invention has the advantages of low cost of the screening method and provides arsenic-resistant fermentation strain resources for repairing arsenic-polluted environment by microorganisms.
Drawings
FIG. 1 shows a screening method and a screening process of arsenic-resistant fermentation bacteria.
FIG. 2 is a graph showing the growth of arsenic tolerant fermenting bacteria under trivalent arsenic stress according to the present invention.
FIG. 3 is a plot of inorganic trivalent arsenic inhibitory concentration fitting for arsenic resistant fermenting bacteria embodying the invention.
FIG. 4 shows the production of butyric acid by an arsenic-tolerant fermentation bacterium according to the invention.
FIG. 5 shows the lactic acid production of arsenic-tolerant fermentation bacteria embodying the invention.
FIG. 6 is a graph showing the hydrogen production of arsenic resistant fermentation bacteria in a 70mL system embodying the present invention.
Detailed Description
In order to make the purpose and experimental scheme of the invention clearer, the invention is further described, and the invention provides the screening and application of the arsenic-resistant fermentation bacteria aiming at the problem of insufficient resources of the strain for repairing the arsenic-polluted environment by the existing microorganism.
The invention provides an arsenic-resistant zymocyte, in particular to application of arsenic-resistant zymotic clostridium butyricum (Clostridium butyricum) in repairing arsenic-polluted environment, wherein the preservation number of clostridium butyricum (Clostridium butyricum) is CGMCC NO.26434.
The 16s rDNA preferred sequence of the arsenic-resistant clostridium butyricum is shown as follows:
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGATG
AAGCTCCTTCGGGAGTGGATTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTCATAGAGGGGAATAGCCTTTCGAAAGGAAGATTAATACCGCATAAGATAGTAGTATCGCATGGTACAGCAATTAAAGGAGTAATCCGCTATGAGATGGACCCGCGTCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCAACGCCGCGTGAGTGATGACGGCCTTCGGATTGTAAAACTCTGTCTTTAGGGACGATAATGACGGTACCCTAAGGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTAGGTGGATATTTAAGTGGGATGTGAAATACTCGGGCTTAACCTGGGTGCTGCATTCCAAACTGGATATCTAGAGTGCAGGAGAGGAAAGGAGAATTCCTAGTGTAGCGGTGAAATGCGTAGAGATTAGGAAGAATACCAGTGGCGAAGGCGCCTTTCTGGACTGTAACTGACACTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTAGGGGTTGTCATGACCTCTGTGCCGCCGCTAACGCATTAAGTATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTAGACTTGACATCTCCTGAATTACTCTGTAATGGAGGAAGCCACTTCGGTGGCAGGAAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTGTTAGTTGCTACCATTTAGTTGAGCACTCTAGCGAGACTGCCCGGGTTAACCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTACACACGTGCTACAATGGTCGGTACAATGAGATGCAACCTCGCGAGAGTGAGCAAAACTATAAAACCGATCTCAGTTCGGATTGTAGGCTGAAACTCGCCTACATGAAGCTGGAGTTGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTGGCAATACCCAAAGTTCGTGAGCTAACCGGTAAGAG
example 1
The arsenic-resistant clostridium butyricum separating screen is selected from high-arsenic polluted estuary sediment at the downstream of a drain outlet of a zinc plant in calabash island city in the gulf of calabash island city, and the screening method is a method known to a person skilled in the art unless specifically stated.
As shown in FIG. 1, the screening method of the arsenic-resistant fermentation clostridium butyricum provided by the invention comprises the following steps:
activation of flora: inoculating a sediment sample with the mass volume ratio of 0.2% to a sterile anaerobic fermentation medium with the pentavalent arsenic concentration of 60mg/L, and carrying out shaking dark culture at 35 ℃ by a shaking table;
enrichment culture: adding the activated bacterial liquid into a sterile anaerobic fermentation medium with the pentavalent arsenic concentration of 60mg/L according to the volume ratio of 1%, shaking the culture medium in a shaking way at 35 ℃ for dark culture, and repeating for 3 times;
gradient dilution plate coating: diluting the enriched bacterial liquid with 0.1% oxygen-free sterile tryptone solution at a mass-volume ratio of 10 2 -10 8 Take 10 5 -10 8 Coating on RCM semisolid culture medium, and culturing in anaerobic organism incubator at 35deg.C under constant temperature and in dark place;
bacterial strain picking and activating: picking single colony on the flat plate to liquid fermentation culture medium, shaking and dark culturing at 35 ℃;
repeating the plate coating and the strain picking and activating for three times to obtain pure strains;
extracting total DNA;
16s rDNA sequence identification.
Example 2
The application of the arsenic-resistant clostridium butyricum provided by the invention in the restoration of arsenic-polluted environment is described in detail by combining with the examples.
Trivalent arsenic gradient stress experiment: activating the screened arsenic-resistant clostridium butyricum with liquid fermentation medium for 10h, inoculating 1% volume ratio into fresh liquid fermentation medium containing 0, 10, 50, 80mg/L, and measuring absorbance value OD 600 The growth curve (see FIG. 2) was obtained, and it can be seen that the trivalent arsenic concentration of 80mg/L had no significant effect on the absorbance value of the arsenic-tolerant clostridium butyricum.
Example 3
Arsenic-tolerant clostridium butyricum trivalent arsenic inhibition concentration experiment: activating the screened arsenic-resistant clostridium butyricum with a fermentation medium for 10 hours, and inoculating 1% of the arsenic-resistant clostridium butyricum into a fermentation medium containing 0, 100, 150 and 200mg/L inorganic trivalent arsenic (As) 3+ ) Fitting of inhibitory concentration (see FIG. 3) was performed by growth peak dry weight measurement experiments in fresh liquid fermentation medium of (A), it can be seen that arsenic-tolerant Clostridium butyricum inorganic trivalent arsenic (As 3+ ) The 50% inhibitory concentration, 90% inhibitory concentration (IC 50, IC 90) was about 120, 200mg/L.
Example 4
Experiments of producing butyric acid, lactic acid and hydrogen by arsenic-resistant fermentation clostridium butyricum: activating the screened arsenic-resistant clostridium butyricum by using a fermentation medium for 10 hours, inoculating 1% of the arsenic-resistant clostridium butyricum into the liquid fermentation medium by volume ratio, taking a headspace gas to measure the volume at normal pressure, taking a supernatant, measuring the butyric acid production condition of the supernatant by using a high performance liquid chromatograph (see figure 4), measuring the lactic acid production condition of the supernatant by using a biochemical analyzer (see figure 5), measuring the hydrogen production concentration by using a gas chromatograph, and finally obtaining the hydrogen yield (see figure 6).
Claims (2)
1. Clostridium butyricum (Clostridium butyricum) CXL-1 is characterized in that clostridium butyricum is preserved in China general microbiological culture Collection center (CGMCC) No.26434 in the month 1 of 2023.
2. Use of clostridium butyricum according to claim 1 for the microbial remediation of an arsenic contaminated environment.
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Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008111608A1 (en) * | 2007-03-14 | 2008-09-18 | National University Corporation Yokohama National University | Novel hydrogen-producing bacterium |
| CA3136385A1 (en) * | 2019-04-10 | 2020-10-15 | Native Microbials, Inc. | Methods and systems for stabilization and preservation of microbes |
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2023
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| CN102041274A (en) * | 2010-12-14 | 2011-05-04 | 东南大学 | Method for producing hydrogen by fermenting special anaerobic clostridium butyricum |
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