CN105255782A - Cellulosimicrobium cellulans with reducing capacity on hexavalent chromium and application - Google Patents

Cellulosimicrobium cellulans with reducing capacity on hexavalent chromium and application Download PDF

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CN105255782A
CN105255782A CN201510777941.0A CN201510777941A CN105255782A CN 105255782 A CN105255782 A CN 105255782A CN 201510777941 A CN201510777941 A CN 201510777941A CN 105255782 A CN105255782 A CN 105255782A
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chromium
soil
hexavalent chromium
bacterium
strain
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CN105255782B (en
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王强锋
侯勇
夏中梅
曾显斌
朱彭玲
胡甦
曾芸
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SAAS BIOTECHNOLOGY AND NUCLEAR TECHNOLOGY RESEARCH INSTITUTE
Sichuan Lanyue Science & Technology Co ltd
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Abstract

The invention discloses cellulosimicrobium cellulans with the reducing capacity on hexavalent chromium and application for solving the problem of chromium pollution in soil. The Latin name of a cellulosimicrobium cellulans strain is Cellulosimicrobium sp., and the preservation number is CGMCC NO 11511; the strain with the hexavalent chromium reducing capacity is obtained by collecting the soil polluted by chromium slag and then performing screening, separating and purifying. The strain can reduce the hexavalent chromium with the high toxicity into trivalent chromium with the lower toxicity, reduce the free property of chromium ions, relieve the stress effect of chromium on plants and reduce the chromium absorbing amount of the plants; in addition, the strain can fix nitrogen, be easily and fixedly planted in plant rhizosphere and promote the crop growth. The strain can be used for repairing the chromium-polluted soil and preventing chromium pollution from entering a food chain and is especially suitable for the actual planting national conditions that planting is performed on the polluted soil while repairing is performed in China, and the better application prospect is achieved in a chromium pollution treatment aspect in wastewater treatment.

Description

Sexavalent chrome is had to fiber bacterium and the purposes of reducing power
technical field:
The present invention relates to hexavalent chromium polluted microorganism remediation field, be specifically related to a strain has efficient reducing power bacterial strain and application method to sexavalent chrome.
background technology:
Because the mankind are movable increasing to the exploitation of metallic ore, smelting, processing and industrial production etc., make plurality of heavy metal such as chromium, lead, cadmium, mercury etc. enter in soil, cause serious environmental pollution, cause the extensive concern of researchist.Heavy metal not easily with water leaching, can not be degraded by microorganisms in soil, and by food chain enrichment in vivo, is even converted into the compound that toxicity is stronger.
Chromium and compound is important industrial raw material, and be widely used in the industries such as plating, process hides, printing and dyeing, the discharge of a large amount of chromate waste water, waste gas and waste residue causes severe contamination to environment.Chromium, as one of common heavy metal contaminants, exists with multiple valence state in the environment, and wherein main based on trivalent chromium and sexavalent chrome, both show distinct behavioural characteristic in the environment.Sexavalent chrome strong toxicity, water-soluble, be easily absorbed by plants, main with CrO in the environment 4 2-, Cr 2o 7 2-exist, not easily adsorbed by soil particle, transport property is strong, easily causes groundwater pollution, produces mutagenesis and carcinogenic effect to organism.Trivalent chromium toxicity is little, and main in the environment exist with the chromium hydroxide of indissoluble, active poor, comparatively light to the toxic action of plant, there are some researches show that chromic toxicity is chromic about 100 times.Trivalent chromium in environment and sexavalent chrome can be transformed mutually by redox reaction.Traditional repairing and treating method at present for chromium-polluted soil has immobilization/stabilization, chemical reduction, chemical leaching, electro reclamation and phytoremediation etc., but large multipair site requirements is higher, limits more; For the process of industrial wastewater containing chromium, then there are ferrous sulfate method, ion exchange method and microbiological process.And microbiological treatment technology is compared with conventional processing technique, there is the advantages such as quick, safety, expense are low, be called as emerging environmental friendliness substitute technology.
Therefore; separate microorganism from chromium-polluted soil; and make full use of the feature of soil itself; be applied to contaminated soil and water remediation targetedly; it is a kind of ideal restorative procedure; avoid and produce secondary pollution to while pollution amelioration, economic benefit and ecological benefits are remarkable, for ecological environmental protection and Sustainable development significant.
From 20 century 70s, researchist finds the sexavalent chrome that some microorganisms can reduce in chromate waste water, and people have done a large amount of correlative study in the chromic field of micro-reduction afterwards, and isolates the multiple microorganism with hexavalent chrome reduction ability.The microorganism that can reduce to sexavalent chrome can carry out collection and be separated from the place that pollution of chromium is serious, as chromium slag muck puts field, tanning sewage discharge point, mining site and electroplating wastewater etc., and show different sexavalent chrome tolerances and reducing power, mainly contain achromobacter, intestinal bacteria, thiobacillus, bacillus, desulfovibrio, micrococci and pseudomonas etc.
summary of the invention:
The object of the present invention is to provide a kind of fiber bacterium sexavalent chrome to reducing power of Effective Anti sexavalent chrome toxicity.
Another object of the present invention is to provide the application of above-mentioned fiber bacterium in repairing hexavalent chromium polluted soil and chromyl wastewater treatment.
Invention is achieved in that
Sexavalent chrome is had to the fiber bacterium of reducing power, its Latin literary fame Cellulosimicrobiumsp., depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.Preservation date: on October 16th, 2015, deposit number: CGMCCNO.11511.
Described fiber bacterium is in the application of repairing hexavalent chromium polluted agricultural land soil.
Described fiber bacterium is in the application of repairing hexavalent chromium polluted soil, described farm crop are paddy rice, wheat, corn, rape, veterinary antibiotics, the microbiobacterial agent that to use with fiber bacterium in proportion of crop planting process be main component, effectively can reduce chromic content in soil, reduce chromium to enter in plant body, and then stop chromium by the edible organ contaminated food of plant.
The described application of fiber bacterium in hexavalent chromium polluted wastewater treatment.
The described application of fiber bacterium in hexavalent chromium polluted wastewater treatment, in this bacterial strain reduction of hexavalent chromium process, pH value is 5-9; Initial hexavalent chromium concentration is no more than 300mg/kg; Add a kind of or wherein several arbitrarily combination in sucrose, glucose, fructose or starch in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add a kind of or wherein several arbitrarily combination in ammonium sulfate, saltpetre, ammonium chloride or yeast extract paste in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add calcium carbonate in pollution of chromium waste water, addition is by wastewater quality 0.1-0.5%.
Fiber bacterium Cellulosimicrobiumsp., numbering LY22 certainly of the present invention, detailed separation screening step is as follows:
(1) sample collecting: take chromium slag contaminated soil 0-20cm soil layer sample in October, 2012.
(2) enrichment culture: (liquid culture based component is: sucrose 10gL in the liquid nutrient medium of sterilizing to take 10g soil sample -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.4gL -1, pH7.0) in, draw 10ml nutrient solution after 28 DEG C of incubator shaking culture 48h and carry out Secondary Culture, go down to posterity 5 times.
(3) sexavalent chrome resistant strain is separated: draw 10ml enrichment culture liquid in the triangular flask that 90ml stroke-physiological saline solution is housed, in 28 DEG C of shaking table vibration 30min, draw in 1ml to 9ml stroke-physiological saline solution again and be progressively diluted to different gradient, get different dilution gradient bacteria suspension 0.1ml respectively and be coated on containing 200mgL -1chromic have nitrogen to improve solid medium (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.8gL -1, agar 18gL -1, pH7.0) and on flat board, cultivate 3 days in 28 DEG C of incubators, the bacterial strain grown is sexavalent chrome resistant strain.
(4) line of sexavalent chrome resistant strain is separated: bacterium colonies different for the sexavalent chrome resistant strain picking obtained in step (3) is carried out plate loop method, and carries out the checking of multiple sieve until be separated to pure bacterium colony, by inoculation to slant medium (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, agar 18gL -1, pH7.0) on, put in 4 DEG C of refrigerators for subsequent use after bacterium grows, and preserve with Freezing Glycerine pipe a in-80 DEG C.
(5) fiber bacterium screening: sexavalent chrome resistant strain is cultured to logarithmic phase, and being seeded to hexavalent chromium concentration by 1% inoculum size is 100mgL -1liquid nutrient medium (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.4gL -1, pH7.0), centrifugal after putting 28 DEG C of incubator shaking culture 48h, with Cr in diphenylcarbazide colorimetric method for determining supernatant liquor 6+content, with the nutrient solution of not inoculating strain for contrast.Measurement result shows: compared with the control, and after inoculating strain, the sexavalent chrome of 92% is reduced into trivalent chromium, and hexavalent chrome reduction rate is high.
(6) bacterial strain nitrogen fixing capacity measures: selected by bacterial strain and receive Ashby nitrogen-free agar flat board (N.F,USP MANNITOL 10gL -1, KH 2pO 40.2gL -1, MgSO 47H 2o0.2gL -1, NaCl0.2gL -1, CaSO 42H 2o0.2gL -1, CaCO 35gL -1, agar 18gL -1, pH7.2) on, be inverted for 28 DEG C and cultivate 5d, bacterial strain energy normal growth, transfer 5 times later still can normal growth.
Sexavalent chrome is had to the fiber bacterium of efficient reducing power, there is following feature:
1) colony morphology characteristic: bacterium colony is irregular, opaque, white on Gause I substratum.
2) morphological features: utilize electron microscopic morphology to identify and Gram stain analysis.Feature is as follows: Gram-positive, elongated rod shape.
3) physiological and biochemical property: methyl red (+), V.P react (+), catalase (+), oxydase (+), gelatin hydrolysis (+), indoles (-), Citrate trianion (+), starch (+).
4) to hexavalent chrome reduction ability:
The present invention in laboratory conditions the result shows that this fiber bacterium has efficient reductive action to sexavalent chrome, can be used for the biological restoration of heavy metal chromium pollution soil and water body.
5) molecular biology identification: utilize 16SrDNA to identify, adopts universal primer 27F and 1492R to increase its 16SrDNA checking order, then with the comparison of NCBIGenBank nucleic acid database, homology is 99%, is accredited as fiber bacterium Cellulosimicrobiumsp..
6) be LY22 by the Strain Designation that above-mentioned separation obtains, through Morphological observation and molecular biology identification, confirm that this bacterium is fiber bacterium (Cellulosimicrobiumsp.).This bacterial strain is delivered China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on October 16th, 2015 and is registered preservation, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.Its preserving number is CGMCCNO.11511.Classification And Nomenclature is: fiber bacterium (Cellulosimicrobiumsp.).
The present invention is by carrying out potted plant amplification planting experiment with this bacterial strain of hexavalent chromium polluted soil inoculation under booth condition, result shows that this bacterium has efficient reductive action to sexavalent chrome, chromic content in soil can be reduced, efficient prevention chromium enters in plant materials, slow down sexavalent chrome to plant coercion, promote plant growth.In laboratory conditions, hexavalent chromium polluted waste water is inoculated this bacterial strain and is processed, and result shows that this bacterium effectively can reduce sexavalent chrome in water body, and water body becomes clear by " Huang ", and hexavalent chrome reduction rate reaches more than 92%.This bacterial strain has good application prospect to hexavalent chromium polluted soil, bioremediation of waters aspect.
Below save the purposes that embodiment further illustrates fiber bacterium provided by the invention.
embodiment:
Embodiment 1:
The preparation method of fiber bacterium (Cellulosimicrobiumsp.) LY22, concrete operations are as follows:
(1) sample collecting: take chromium slag contaminated soil 0-20cm soil layer sample in October, 2012.
(2) enrichment culture: take 10g soil sample in the liquid nutrient medium of sterilizing, draws 10ml nutrient solution after 28 DEG C of incubator shaking culture 48h and carries out Secondary Culture, go down to posterity 5 times.Liquid culture based component is: sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.4gL -1, pH7.0.
(3) sexavalent chrome resistant strain is separated: draw 10ml enrichment culture liquid in the triangular flask that 90ml stroke-physiological saline solution is housed, in 28 DEG C of shaking table vibration 30min, draw in 1ml to 9ml stroke-physiological saline solution again and be progressively diluted to different gradient, get different dilution gradient bacteria suspension 0.1ml respectively and be coated on containing 200mgL -1chromic (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.8gL -1, agar 18gL -1, pH7.0) on, cultivate 3 days in 28 DEG C of incubators, the bacterial strain grown is sexavalent chrome resistant strain.
(4) line of sexavalent chrome resistant strain is separated: bacterium colonies different for the sexavalent chrome resistant strain picking obtained in step (3) is carried out plate loop method, and carry out the checking of multiple sieve until be separated to pure bacterium colony, by inoculation to slant medium (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, agar 18gL -1, pH7.0) on, putting 4 DEG C of refrigerators after bacterium grows is the finished product, and preserves a for subsequent use in-80 DEG C of preservations with Freezing Glycerine pipe.
(5) fiber bacterium screening: sexavalent chrome resistant strain is cultured to logarithmic phase, and being seeded to hexavalent chromium concentration by 1% inoculum size is 100mgL -1liquid nutrient medium (sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, potassiumchromate 0.4gL -1, pH7.0), centrifugal after putting 28 DEG C of incubator shaking culture 48h, with Cr in diphenylcarbazide colorimetric method for determining supernatant liquor 6+content, with the nutrient solution of not inoculating strain for contrast.Measurement result shows: compared with the control, and after inoculating strain, the sexavalent chrome of 92% is reduced into trivalent chromium.
(6) bacterial strain nitrogen fixing capacity measures: received by bacterial strain point on Ashby nitrogen-free agar flat board, is inverted for 28 DEG C and cultivates 5d, bacterial strain energy normal growth, and transferring 5 times later still can normal growth.Ashby nitrogen-free agar composition is: N.F,USP MANNITOL 10gL -1, KH 2pO 40.2gL -1, MgSO 47H 2o0.2gL -1, NaCl0.2gL -1, CaSO 42H 2o0.2gL -1, CaCO 35gL -1, agar 18gL -1, pH7.2.
Sexavalent chrome is had to the fiber bacterium of efficient reducing power, this bacterial strain has following feature:
1) colony morphology characteristic: bacterium colony is irregular, opaque, white on Gause I substratum.
2) morphological features: utilize electron microscopic morphology to identify and Gram stain analysis.Feature is as follows: Gram-positive, elongated rod shape.
3) physiological and biochemical property: methyl red (+), V.P react (+), catalase (+), oxydase (+), gelatin hydrolysis (+), indoles (-), Citrate trianion (+), starch (-).
4) to hexavalent chrome reduction ability:
The present invention in laboratory conditions the result shows that this fiber bacterium has efficient reductive action to sexavalent chrome, can be used for the biological restoration of heavy metal chromium pollution soil and water body.
5) molecular biology identification: utilize 16SrDNA to identify, adopts universal primer 27F and 1492R to increase its 16SrDNA checking order, then with the comparison of NCBIGenBank nucleic acid database, homology is 99%, is accredited as fiber bacterium Cellulosimicrobiumsp..
Embodiment 2:
Fiber bacterium is going back sexavalent chrome in native soil, intercepts chromium and enters in plant body, and the application of Promoting plant growth, concrete operations are as follows:
1, material
Bacterial strain: fiber bacterium (Cellulosimicrobiumsp.) LY22, it is CGMCCNO.11511 that preserving number is registered at China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC).
Planting medium: hexavalent chromium polluted soil, hexavalent chromium polluted concentration 30mgkg -1
Planting container: volume is the cultivation tray of 2 liters
Vegetable seedling: Plantula Brassicae chinensis seedling
2, working method and step:
(1) bacterium solution preparation: by fiber bacterium (Cellulosimicrobiumsp.) LY22 inclined-plane inoculation in the triangular flask that nitrogen improvement liquid nutrient medium is housed, 35 DEG C, 150r/min shaking culture, when under 600nm wavelength, absorbancy reaches 0.6 ~ 0.8 to bacterium liquid, be inoculated in the fermentor tank that nitrogen improved culture medium is housed by 1% ~ 5% inoculum size (V/V), 35 DEG C, stirring velocity 180r/min, logical sterile air ferments, fermentation 36h, in bacterium liquid, effective fiber bacterium viable count is not less than 5 × 10 9individual/milliliter.Nitrogen improved culture medium composition is had to be: sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, agar 18gL -1, pH7.0.
(2) solid fungicide preparation: regulate bacterium liquid pH to 7.2-7.4, rice chaff, white carbon black, light calcium carbonate are mixed with into solid fungicide with bacterium liquid by a certain percentage, in microbial inoculum, the mass ratio of rice chaff, white carbon black, light calcium carbonate and bacterium liquid is 2:2:2:1, solid fungicide water content is lower than 15%, and the living bacteria count of fiber bacterium is not less than 2 × 10 8individual/g.
(3) planting medium prepares: with every basin 2 kilograms of hexavalent chromium polluted soil;
(4) soil remediation process and shoot transplanting equipment: will the usage quantity of per kilogram soil application 50g solid fungicide be pressed, by soil and solid fungicide Homogeneous phase mixing, water and make Soil conservation moistening, after balancing 2 weeks, transplant in the soil after Plantula Brassicae chinensis seedling to process, and soil sampling determination of hexavalent chromium content; Arrange simultaneously and do not use the soil of microbial inoculum to compare;
(4) Plantula Brassicae chinensis plantation daily administration, measures the biomass of Plantula Brassicae chinensis, and gets measuring blade chromium content to harvesting time.Result is as following table:
Content of 6-valence Cr ions (mg/kg) in soil Chromium content (mg/kg) in blade Biomass of individual tree (g)
Contrast 29.21±2.11 0.62±0.10 46.52+3.47
Process 8.31±1.52 0.21±0.06 72.23+5.36
Effect Hexavalent chrome reduction rate=72.41% Chromium rejection rate=66.13% Stimulation ratio=55.27%
Note: chromic content × 100% in hexavalent chrome reduction rate==(in contrast soil in chromic content-process soil chromic content) ÷ contrast soil
In the plant-growth organ of the chromium rejection rate=content of chromium (in the plant-growth organ of the content-process of chromium in the plant-growth organ of contrast) ÷ contrast every content × 100%
Plant biomass of individual tree × 100% that stimulation ratio=(the plant biomass of individual tree of the plant biomass of individual tree-contrast of process) ÷ contrasts
Embodiment 3:
Fiber bacterium is chromic application in reducing waste water, and concrete operations are as follows:
1, material
Bacterial strain: fiber bacterium (Cellulosimicrobiumsp.) LY22, it is CGMCCNO.11511 that preserving number is registered at China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC).
Medium: hexavalent chromium polluted waste water, hexavalent chromium polluted concentration 200mgkg -1
Container: volume is the plastic tank of 100 liters
2, working method and step
(1) bacterium solution preparation: with embodiment 2.
(2) medium prepares: fill the chromyl waste water of 50L inside each plastic tank.
(3) process: regulate wastewater pH to be 5-9, add a kind of or wherein several arbitrarily combination (shared by glucose, sugarcane sugar and starch, mass ratio is 2:2:1) in sucrose, glucose, fructose or starch in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add a kind of or wherein several arbitrarily combination in ammonium sulfate, saltpetre, ammonium chloride or yeast extract paste (shared by ammonium sulfate and yeast extract paste, mass ratio is 1:1) in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add calcium carbonate in pollution of chromium waste water, addition is by wastewater quality 0.1-0.5%; (preparation method is with (1) bacterium solution preparation: by fiber bacterium (Cellulosimicrobiumsp.) LY22 inclined-plane inoculation in the triangular flask that nitrogen improvement liquid nutrient medium is housed to add bacterium liquid by 5%-10% of wastewater quality, 35 DEG C, 150r/min shaking culture, when absorbancy reaches 0.6 ~ 0.8 to bacterium liquid under 600nm wavelength, be inoculated into by 1% ~ 5% inoculum size (V/V) and nitrogen improved culture medium be housed (medium component is: sucrose 10gL -1, K 2hPO 42gL -1, (NH 4) SO 41gL -1, MgSO 47H 2o0.5gL -1, CaCO 30.5gL -1, yeast extract paste 0.5gL -1, NaCl0.1gL -1, agar 18gL -1, pH7.0) fermentor tank in, 35 DEG C, stirring velocity 180r/min, logical sterile air ferments, fermentation 36h, in bacterium liquid, effective fiber bacterium viable count is not less than 5 × 10 9individual/milliliter).Mix after above-mentioned materials interpolation is complete, content of 6-valence Cr ions in wastewater measurement after aeration 96h.Arrange the waste water be left intact is contrast simultaneously.Result is as following table:
Content of 6-valence Cr ions (mg/kg) in waste water
Contrast 198.35±2.76
Process 15.32±1.03
Hexavalent chrome reduction rate (%) 92.28
Show according to above-mentioned test effect, fiber bacterium (Cellulosimicrobiumsp.) LY22 heavy metal sexavalent chrome provided by the invention has very strong reducing power, reduce content of 6-valence Cr ions in soil, chromium is entered in plant body there is significant blocking effect, effectively reduce objectionable impurities accumulation in farm crop, and can plant growth be promoted, be particularly suitable for China's actual plantation national conditions in contaminated soil top plantation limit reparation.In addition, in chromyl wastewater treatment, there is good effect.Therefore, have a good application prospect in the microorganism remediation of hexavalent chromium polluted soil and water.
Above-described embodiment is further described foregoing of the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.

Claims (5)

1. pair sexavalent chrome has the fiber bacterium of reducing power, it is characterized in that its Latin literary fame Cellulosimicrobiumsp., depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation date: on October 16th, 2015, deposit number: CGMCCNO.11511.
2. fiber bacterium as claimed in claim 1 is in the application of repairing hexavalent chromium polluted agricultural land soil.
3. fiber bacterium according to claim 2 is in the application of repairing hexavalent chromium polluted soil, it is characterized in that described farm crop are paddy rice, wheat, corn, rape, veterinary antibiotics, the microbiobacterial agent that to use with fiber bacterium in proportion of crop planting process be main component, effectively can reduce chromic content in soil, reduce chromium to enter in plant body, and then stop chromium by the edible organ contaminated food of plant.
4. the application of fiber bacterium according to claim 1 in hexavalent chromium polluted wastewater treatment.
5. the application of fiber bacterium according to claim 4 in hexavalent chromium polluted wastewater treatment, is characterized in that: in this bacterial strain reduction of hexavalent chromium process, pH value is 5-9; Initial hexavalent chromium concentration is no more than 300mg/kg; Add a kind of or wherein several arbitrarily combination in sucrose, glucose, fructose or starch in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add a kind of or wherein several arbitrarily combination in ammonium sulfate, saltpetre, ammonium chloride or yeast extract paste in pollution of chromium waste water, addition is by wastewater quality 0.1-1%; Add calcium carbonate in pollution of chromium waste water, addition is by wastewater quality 0.1-0.5%.
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CN108017136A (en) * 2017-12-14 2018-05-11 广州市香港科大霍英东研究院 A kind of method that poisonous Cr VI is changed into trivalent chromium using organic matter
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CN105513737A (en) * 2016-01-21 2016-04-20 烟台首钢磁性材料股份有限公司 Preparation method of sintered neodymium-iron-boron magnet without containing heavy rare earth elements
US11062827B2 (en) 2016-01-21 2021-07-13 Yantai Shougang Magnetic Materials Inc. Sintered magnet composition without heavy rare earth element and a method of making the sintered magnet
CN105855290A (en) * 2016-04-11 2016-08-17 浙江大学 Fungus repair method for severe chromium-contaminated soil
CN106085900B (en) * 2016-06-07 2019-05-14 中国科学院生态环境研究中心 Fiber germ and its application that chromate waste water is handled
CN106085900A (en) * 2016-06-07 2016-11-09 中国科学院生态环境研究中心 Fiber germ and the application that chromate waste water is processed thereof
CN106011009B (en) * 2016-06-15 2019-05-14 中国科学院生态环境研究中心 Alcaligenes and its application that chromate waste water is handled
CN106011009A (en) * 2016-06-15 2016-10-12 中国科学院生态环境研究中心 Alcaligenes sp. GTM2 and application thereof to chromium-containing wastewater treatment
CN109647875A (en) * 2017-10-11 2019-04-19 庄正 Application of the fiber germ GTM1 in chromium-polluted soil reparation
CN108017136A (en) * 2017-12-14 2018-05-11 广州市香港科大霍英东研究院 A kind of method that poisonous Cr VI is changed into trivalent chromium using organic matter
CN109182178A (en) * 2018-09-12 2019-01-11 山西省农业科学院果树研究所 One plant of bacterial strain and its application in reparation mild or moderate chromium-polluted soil in situ with chromium tolerance and Cr (VI) removal ability

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