CN112662598A - Fiber fingi micro-bacterium, microbial inoculum comprising fiber fingi micro-bacterium, preparation method and application - Google Patents
Fiber fingi micro-bacterium, microbial inoculum comprising fiber fingi micro-bacterium, preparation method and application Download PDFInfo
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Abstract
The invention provides a strain of Fengshi fiber micro bacteria (Cellulosimicrobumfunkei) SL-1, with the preservation number as follows: CGMCC No.20950 belongs to the technical field of microbial preparations. The Fengshi fiber microbacterium SL-1 has high tolerance to diuron with high concentration, can grow in a culture medium with diuron as a unique carbon source, and can degrade over 90 percent of diuron of 200mg/kg within 5d (120 h). The method for degrading diuron by using the Fengshi fiber micro-bacterium SL-1 strain belongs to a biological metabolism method, and does not produce secondary pollution. The invention provides an efficient and excellent strain for efficiently degrading diuron and provides a high-quality strain resource for repairing and treating diuron-polluted soil in agricultural areas for a long time.
Description
Technical Field
The invention relates to the technical field of microbial preparations, in particular to a fiber fense microbe, a microbial inoculum containing the fiber fense microbe, a preparation method and application.
Background
Diuron (diuron) is a low-toxicity substituted urea herbicide, and has a systemic conduction effect and a certain contact action. After the medicament is absorbed by roots or leaves of plants, photosynthesis is inhibited, so that the leaves are green, the leaf tips and edges are faded, and the plants die due to lack of nutrition. Is mainly used for preventing and killing annual gramineous weeds in crops such as cotton, soybean, tomato and the like. The degradation mechanism of diuron is mainly biodegradation and photodegradation, and biodegradation is an important strategy for reducing harmful compounds. At present, the microbial method for degrading pesticides mainly comprises the following steps: the method comprises the steps of screening and separating strains with excellent properties and good growth from soil seriously polluted by pesticides for a long time, carrying out directed culture, and carrying out artificial mutation breeding, genetic engineering, construction of engineering strains and other means on the basis. However, related researches on diuron microbial degradation at home and abroad are few, and no report or record is provided for diuron degradation by using the microfine fibrates.
Disclosure of Invention
The invention aims to provide a fiber fense micro-bacterium, a microbial inoculum comprising the fiber fense micro-bacterium, a preparation method and application.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a strain of Fengshi fiber micro bacteria (Cellulosimicrobium funkei) SL-1, with the preservation number: CGMCC No. 20950.
The invention also provides a microbial inoculum comprising the fibraurea fimbriatus.
Preferably, the effective viable count of the fibraurea fipronil in the microbial inoculum is 2.55 multiplied by 108CFU/mL。
The invention also provides a preparation method of the microbial inoculum in the scheme, which comprises the following steps:
inoculating the fiber-fingi micro bacteria to an LB liquid culture medium, and performing amplification culture to obtain a microbial inoculum;
the initial concentration of the Fengshi fiber micro-bacteria is (1-9) multiplied by 109cfu/mL; the inoculation amount of the fiber fingi micro-bacteria is 10-20% of the volume of the LB liquid culture medium.
Preferably, the temperature of the amplification culture is 28-32 ℃; the rotation speed of the amplification culture is 150-200 rpm; the time of the amplification culture is 3-5 days.
The invention also provides application of the fense fiber micro-bacteria or the microbial inoculum in the scheme in degrading diuron.
The invention also provides application of the fense fiber micro-bacteria or the microbial inoculum in the scheme in improving the degradation rate of diuron.
The invention also provides application of the fibraurea fimbriata or the microbial inoculum in the scheme in reducing plant damage caused by diuron and/or promoting plant growth.
Preferably, when the diuron is located in soil, the application comprises the steps of:
inoculating a microbial inoculum containing the said F.finnii in the soil;
adjusting the water content of the soil to be 55-65%.
Preferably, the inoculation mode comprises spraying or drip irrigation of the microbial inoculum with water.
The invention has the beneficial effects that: the invention provides a strain of Fengshi fiber micro bacteria (Cellulosimicrobium funkei) SL-1, with the preservation number: CGMCC No. 20950. The Fengshi fiber microbacterium SL-1 has high tolerance to diuron with high concentration, can grow in a culture medium with diuron as a unique carbon source, and can degrade over 90 percent of diuron of 200mg/kg within 5d (120 h). The method for degrading diuron by using the Fengshi fiber micro-bacterium SL-1 strain belongs to a biological metabolism method, and does not produce secondary pollution. The invention provides an efficient and excellent strain for efficiently degrading diuron and provides a high-quality strain resource for repairing and treating diuron-polluted soil in agricultural areas for a long time.
Biological preservation Instructions
The Fengshi fiber micro bacteria (Cellulosimicrobium funkei) SL-1 is preserved in the common microorganism center of China Committee for Culture Collection of Microorganisms (CCM) at 26 months 10 and 26 days 2020, and is No. 3 of Xilu No. 1 of Beijing, Guangyang province, with the preservation number: CGMCC No. 20950.
Drawings
FIG. 1 shows actinomycete SL-1 isolated in example 1;
FIG. 2 is a morphological diagram of a single colony of actinomycete SL-1 obtained by the fractionation in example 1;
FIG. 3 is a graph showing the gram staining results of the strain of example 1: gram staining positive;
FIG. 4 is a phylogenetic tree;
FIG. 5 shows the results of investigation of culture conditions for actinomycetes SL-1;
FIG. 6 shows the growth of cotton seedlings under different treatments; wherein A is CK clear water contrast; b is diuron content of 500mg/kg, and no strain is inoculated; c is agent + SL-1.
Detailed Description
The invention provides a strain of Fengshi fiber micro bacteria (Cellulosimicrobium funkei) SL-1, with the preservation number: CGMCC No.20950, collected in 2018 in the general area of stone of the northern spring town of Stone river.
In the present invention, the said Microbacterium finnii (Cellulosimicrobium funkei) SL-1 belongs to the genus Cellulosimicrobium (Cellulosimicrobium sp.); the Weibull fiber microbacterium SL-1 is highly tolerant to diuron with high concentration and can grow in a culture medium with diuron as a sole carbon source.
The invention also provides a microbial inoculum comprising the fibraurea fimbriatus.
In the present invention, the effective viable count of the F.faecalis in the microbial agent is preferably 2.55X 108CFU/mL。
The invention also provides a preparation method of the microbial inoculum in the scheme, which comprises the following steps:
inoculating the fiber-fingi micro bacteria to an LB liquid culture medium, and performing amplification culture to obtain a microbial inoculum;
the initial concentration of the Fengshi fiber micro-bacteria is (1-9) multiplied by 109cfu/mL; the inoculation amount of the fiber fingi micro-bacteria is 10-20% of the volume of the LB liquid culture medium. In the present invention, the initial concentration of said F.finnii is preferably 5.6X 109cfu/mL; the inoculum size of said micro-bacteria of Fibrel was 15% of the volume of LB liquid medium. In the invention, the LB liquid culture medium takes water as a solvent and comprises the following components in mass concentration: 8-12 g/L of tryptone, 3-8 g/L of yeast extract and 8-12 g/L of NaCl.
In the present invention, the amplification medium preferably comprises the following components in mass concentrations: diuron 500mg/L, tryptone 10g/L, yeast extract 5g/L and NaCl 10 g/L.
In the invention, the temperature of the amplification culture is preferably 28-32 ℃, and more preferably 30 ℃; the rotation speed of the amplification culture is preferably 150-200 rpm, and more preferably 180 rpm; the enlarged culture is more preferably performed in a dark culture; the humidity of the expansion culture is preferably 55% to 65%, and more preferably 60%. (ii) a The time for the amplification culture is preferably 3-5 d, and more preferably 4 d.
The invention also provides application of the fense fiber micro-bacteria or the microbial inoculum prepared by the preparation method in degrading diuron.
The invention also provides application of the fense fiber micro-bacteria or the microbial inoculum prepared by the preparation method of the microbial inoculum in the scheme in improving the degradation rate of diuron.
The invention also provides application of the fibraurea fimbriata or the microbial inoculum in the scheme in reducing plant damage caused by diuron and/or promoting plant growth.
In the invention, the concentration of the diuron is preferably less than or equal to 1000mg/kg, because the diuron with high concentration in the soil can inhibit the growth of degradation strains, thereby reducing the degradation efficiency.
In the present invention, when the diuron is located in the soil, the application comprises the steps of: inoculating a microbial inoculum containing the said F.finnii in the soil;
adjusting the water content of the soil to be 55-65%.
In the present invention, the water content of the soil is preferably 60%.
In the present invention, the inoculation mode preferably includes spraying or dripping the microbial inoculum with water.
In the present invention, the inoculation amount of the microbial inoculum is 8% to 12% of the volume of the soil, and more preferably 10%.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The test methods used in the following examples are all conventional methods unless otherwise specified.
Materials, reagents and the like used in the following examples were commercially available unless otherwise specified.
The media used in the following examples are as follows:
A. inorganic salt culture medium: MgSO (MgSO)4·7H2O 0.2g，KH2PO40.5 g，(NH4)2SO41 g，NaCl 0.5g，K2HPO41.5 g, 1000mL of distilled water, and pH 7.0-7.2.
B. Beef extract peptone medium: 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 1000mL of distilled water and pH 7.0-7.2.
C. Enrichment culture medium: diuron is added into the inorganic salt culture medium at the required concentration.
LB medium: 10g of tryptone, 5g of yeast extract, 10g of NaCl and 1000mL of distilled water.
Solid culture medium 18g/L agar powder was added to the above medium.
Example 1
In 2018, soil continuously applied with diuron is collected in a cotton field of a general rocky town stone farm in northern spring town of the river city for more than 10 years, the soil is inoculated into an inorganic salt culture medium for shaking culture and is continuously transferred into an enrichment culture medium containing the diuron, and the formula of the enrichment nutrient solution is as follows: 10g/L of tryptone, 5g/L, NaCl 10g/L of yeast extract, 15g/L of agar powder and 1L of distilled water. Adding 10g of test soil sample into 90mL of enrichment culture solution with diuron concentration of 100mg/L, culturing for 7 days at 30 ℃ and 180r/min, taking liquid with 10% of inoculation volume (v: v), inoculating the liquid into the enrichment culture solution with diuron concentration of 500mg/L, continuously culturing for 7 days, then inoculating the enrichment culture solution into the enrichment culture solution with diuron content of 1000mg/L according to the inoculation amount of 10%, continuously transferring to 1500mg/L, 1800mg/L and 2000mg/L in the way, carrying out plate coating, finding that no bacterial colony appears on the culture solution coating plate when the diuron content is 2000mg/L, and carrying out subsequent test on the single bacterial colony coated when the final diuron content is 1800 mg/L. Before each operation, the microorganism plate coating is needed before the transfer to ensure that the strain is in a survival state (the steps are all carried out on an ultra-clean workbench).
Selecting strains with good growth numbers for storage, and taking the strains as alternative strains and naming the strains as SL-1 to SL-12. Inoculating single colony of 12 candidate strains with serial number into a culture medium of beef extract peptone liquid, placing on a shaker at 30 deg.C and 180r/min, and culturing for 1d to obtain seed liquid. Inoculating 10% inoculation volume of seed liquid into an inorganic salt degradation culture medium with diuron concentration of 100mg/L, placing on a shaking bed for shaking culture, taking degradation bacterial liquid after 3 days, 6 days, 9 days, 12 days and 15 days after shaking culture to detect the diuron content in the degradation bacterial liquid, and calculating the degradation efficiency. And then screening to obtain the target strain SL-1.
Performing morphological identification and physiological and biochemical experiments on the separated and purified bacterial strain SL-1, extracting the genomic DNA of the bacterial strain, performing PCR amplification, sequencing the recovered product, performing sequence analysis on the functional genes of related species, performing homologous sequence search on the obtained sequence in GenBank, and constructing a phylogenetic tree by using DNMAN software.
The colony morphology of the LB medium of the strain SL-1 is shown in figure 1, and the single colony morphology is shown in figure 2; gram-positive for strain SL-1 (FIG. 3); the temperature tolerance range of the strain is 10-40 ℃, and the optimal growth temperature is 28 ℃; the pH tolerance range is pH5.0-8.0, and the optimum pH is 7.0; can tolerate NaCl with the mass fraction of less than 4 percent. Culturing for 4 days at 28 ℃ on a nutrient agar culture medium to form colonies with the diameter of 0.8-1.2 mm, wherein the colonies are light yellow, and the surfaces of the colonies are wet and smooth (shown in figure 4); the cells are in the shape of short rods, have the size of about (0.8-1.0 mu m multiplied by 0.9-1.1 mu m), have no motility and do not form endospores.
The test results of the physiological and biochemical characteristics of the strain SL-1 comprise an API ZYM enzymology test, an API50CH acidogenesis test, a Biolog G3 test and a manual test.
Wherein the API ZYM enzymatic assay comprises 20 physiological and biochemical assay indexes (shown in Table 1); the API50CH acidogenic assay contained 49 physiological and biochemical assay indices (shown in table 2); the Biolog G3 assay contained 96 physiological and biochemical assay indices (shown in table 3); as is clear from the results of the manual experiments in Table 4, the cell morphology of the strain SL-1 is short rod-shaped. Gram staining was positive.
TABLE 1 API ZYM enzymatic test results
+: positive; -: negative; w: weak positive
TABLE 2 API50CH acidogenic test results
+: positive; -: negative; w: weak positive
TABLE 3 Biolog G3 test index
+: positive; -: negative; w: weak positive
TABLE 4 Manual test indexes
| Test items | Results | Test items | Results | Test items | Results | Test items | Results |
| Cell morphology | Short rod shape | Gram stain | Positive for | Hydrolysis of cellulose | - | Liquefaction of gelatin | - |
| H2S production | - | Milk peptone | - | Starch hydrolysis | - |
+: positive; -: negative; w: weak positive
The 16s rDNA sequence of the strain SL-1 is shown in SEQ ID NO. 1 through determination.
The MEGA4.1 software is adopted, the orthotopic ligation method is adopted to display a 16S rDNA phylogenetic tree of a strain 'SL-1' and related species (figure 2), and the SL-1 is identified as follows by combining a plurality of identification analyses of strain morphology, physiological and biochemical data and the like: fibremia fibula (Cellulosimicrobium funkei).
Example 2
By controlling the initial concentration (25, 50, 100, 200, 500mg/L) and inoculation amount (1%, 3%, 5%, 10%, 15% (v: v) of the medicament in the culture medium, the initial concentration is 5.6X 109cfu/mL), temperature (25, 28, 30, 32, 35 ℃), pH (adjusted to pH5.0, 6.0, 7.0, 8.0, 9.0), and an external carbon source (0.0%, 0.5%, 1.0%, 1.5%, 2.0% (m: m) sucrose as an external carbon source), thereby exploring the optimal degradation conditions of actinomycetes SL-1.
The results of this test are shown in FIG. 5.
As shown in figure 5, the actinomycete SL-1 has the highest degradation rate of the strain to the medicament and reaches up to 90.8 percent on the fifth day when the medicament concentration is 200mg/L, the inoculation amount is 5 percent, the pH value is 7 at 30 ℃, and no external carbon source is connected.
Example 3
And selecting a single colony of the SL-1 strain with clear morphology and no pollution in LB, and treating the single colony at 30 ℃ and 180r/min for 24 hours until the liquid is turbid to obtain a bacterial suspension of SL-1.
Adding a proper amount of diuron into soil in a flowerpot to ensure that the concentration of the diuron in the soil is respectively 100, 200, 500 and 1000mg/kg, uniformly mixing the medicament and the soil, inoculating the SL-1 bacterial suspension into the soil in an inoculation amount with the volume fraction of 10 percent respectively, and then adding water into the soil to ensure that the water content in the soil reaches 60 percent (v: m). After the soil treatment is finished, the flowerpot is placed in a constant-temperature incubator at 30 ℃ for dark culture, soil is taken after 0, 3, 6, 9, 12 and 15 days, and the content of diuron in the soil is detected. The results are shown in Table 5.
TABLE 5 Change in diuron content on different days at different concentrations
As shown in Table 5, the degradation rate of the strain to the medicament is gradually reduced along with the increase of the diuron content in the soil, the degradation effect in the soil with the lowest diuron content (100mg/kg) is the fastest, the degradation rate is as high as 63.4%, while the degradation effect of the strain in the soil with the highest diuron content (1000mg/kg) is the slowest, and the degradation rate is only 51.0%. It can be shown that the SL-1 strain can accelerate the degradation of the diuron, and the diuron with high concentration in the soil can inhibit the growth of the degrading strain, thereby reducing the degradation efficiency.
Example 4
1) CK soil without diuron and without degrading strains served as control;
2) so that the diuron content in the soil sample reaches 100mg/kg, and the bacterial strain SL-1 (medicament + SL-1) is inoculated according to the addition amount of 10 percent (v: m);
3) the concentration of diuron in the soil sample is 100mg/kg, but no strain is inoculated.
The three groups of soil tests are repeatedly arranged, soil treatment is carried out in an incubator at 30 ℃ in a dark place for 15 days, 10 cotton seeds are sown in each pot of soil (the cotton seeds are soaked for 12 hours in advance), the emergence rate of the cotton seeds is observed, the physiological indexes (fresh weight, dry weight, root length and the like) of the cotton seeds are measured and counted at 21 days after inoculation, the plant growth condition is observed, the degradation effect is evaluated and analyzed, and the results are shown in table 6 and figure 6.
The results show that SL-1 can better repair soil containing diuron and can relieve phytotoxicity on plants to a certain extent. The cotton seedlings treated by the clear water control and the strain grow well, while the cotton seedlings treated by the diuron have lower emergence rate, short and small plants and wilting cotton seedlings at the later stage (shown in figure 6). While the phytotoxicity phenomenon of cotton seedlings in the soil treated by the bacterial strain SL-1 is relieved and lightened.
Table 6 shows the effect of treatment of diuron residual soil with strain SL-1 on cotton seedlings. As can be seen from Table 6, the rate of emergence of cotton is low, only 50%, when the soil is sown after the soil is subjected to diuron treatment for 15 days and only the diuron treatment is carried out; while the cotton emergence rate after the treatment of the bacterial strain SL-1 is improved to 63.3 percent. And for the data results of the measurement results of fresh weight, dry weight, plant height, root length, root number and the like of the cotton seedlings cultured for 21d, the growth vigor of the cotton seedlings in the clear water control group is the best, and the growth vigor of the cotton seedlings in the drug application soil treated by the strain is inferior to that of the clear water control group, but is obviously superior to that of the drug application treatment group only. The fresh weight of the diuron-treated cotton seedlings is only 0.65g and is 66.32%, and after the diuron-treated cotton seedlings are treated by the bacterial strain SL-1, the fresh weight inhibition rate is reduced by 36.2%; the plant height of the diuron-treated cotton seedlings is only 6.53cm, the plant height inhibition rate is 62.96 percent, and the plant height inhibition rate is reduced by 37.55 percent after the treatment of the bacterial strain SL-1; the root length of the cotton seedlings treated by the diuron is only 3.88cm, the number of beard roots is 14.67, the inhibition rate of the root length is 58.85 percent, and the inhibition rate of the root length is reduced by 27.78 percent after the cotton seedlings are treated by SL-1; and the number of fibrous roots increased to 24.
TABLE 6 Effect of treatment of diuron residual soil with Strain SL-1 on Cotton
As can be seen from Table 6, the bacterial strain SL-1 can have better repair capability on the drug-containing soil and can also reduce the damage of diuron to plants in a certain sense, the data results of the measurement results of fresh weight, dry weight, plant height, root length, root number and the like of cotton seedlings all show that the cotton seedlings in the clear water control group have the best growth vigor, and the cotton seedlings in the drug-applying soil treated by the bacterial strain grow inferior to the cotton seedlings in the clear water control group, but are obviously superior to the drug-applying treatment group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Xinjiang stone science and technology Co., Ltd
<120> one strain of fibula gracilis micro-bacteria, microbial inoculum comprising same, preparation method and application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1583
<212> DNA
<213> Fengshi fiber Microbacterium (Cellulosimicrobium funkei)
<400> 1
gggggtacga cggcagtgat tcgagctcgg tacccgggga tcctctagag attagagttt 60
gatcctggct caggacgaac gctggcggcg tgcttaacac atgcaagtcg aacgatgatg 120
cccagcttgc tgggcggatt agtagcgaac gggtgagtaa cacgtgagta acctgccctt 180
gacttcggga taactccggg aaaccggggc taataccgga tatgagccgc cttcgcatgg 240
ggatggttgg aaagtttttc ggtcagggat gggctcgcgg cctatcagct tgttggtggg 300
gtgatggcct accaaggcga cgacgggtag ccggcctgag agggcgaccg gccatactgg 360
gactgagaca cggcccagac tcctacggga ggcagcagtg gggaatattg cacaatgggc 420
gaaagcctga tgcagcgacg ccgcgtgagg gatgaaggcc ttcgggttgt aaacctcttt 480
cggcagggaa gaagcgcaag tgacggtacc tgcagaagaa gcgccggcta actacgtgcc 540
agcagccgcg gtaatacgta gggcgcaagc gttgtccgga attattgggc gtaaagagct 600
cgtaggcggt ttgtcgcgtc tggtgtgaaa actcgaggct caacctcgag cttgcatcgg 660
gtacgggcag actagagtgc ggtaggggag actggaattc ctggtgtagc ggtggaatgc 720
gcagatatca ggaggaacac cgatggcgaa ggcaggtctc tgggccgcaa ctgacgctga 780
ggagcgaaag catggggagc gaacaggatt agataccctg gtagtccatg ccgtaacgtt 840
gggcactagg tgtggggctc attccacgag ttccgtgccg cagcaaacgc attaagtgcc 900
ccgcctgggg agtacggccg caaggctaaa actcaaagga attgacgggg gcccgcacaa 960
gcggcggagc atgcggatta attcgatgca acgcgaagaa ccttaccaag gcttgacatg 1020
cacgggaagc caccagagat ggtggtctct ttggacactc gtgcacaggt ggtgcatggt 1080
tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aaccctcgtc 1140
ccatgttgcc agcgggttat gccggggact catgggagac tgccggggtc aactcggagg 1200
aaggtgggga tgacgtcaaa tcatcatgcc ccttatgtct tgggcttcac gcatgctaca 1260
atggccggta caaagggctg cgataccgta aggtggagcg aatcccaaaa agccggtctc 1320
agttcggatt ggggtctgca actcgacccc atgaagtcgg agtcgctagt aatcgcagat 1380
cagcaacgct gcggtgaata cgttcccggg ccttgtacac accgcccgtc aagtcacgaa 1440
agtcggtaac acccgaagcc catggcccaa ccgttcgcgg ggggagtggt cgaaggtggg 1500
actggcgatt gggactaagt cgtaacaagg taaccaatcg tcgacctgca ggcatgcaag 1560
cttggcgtaa tcatgtcatt ggc 1583
Claims (10)
1. A strain of Fennelette-guerin (Cellulosimicrobium funkei) SL-1 has the deposit number: CGMCC No. 20950.
2. An agent comprising the fiber fense micro-bacterium of claim 1.
3. The microbial preparation according to claim 2, wherein the effective viable count of the filamentous microfaciens is 2.55X 108CFU/mL。
4. A method for producing the microbial agent according to claim 2 or 3, comprising the steps of:
inoculating the fiber-fingi micro bacteria to an LB liquid culture medium, and performing amplification culture to obtain a microbial inoculum;
the initial concentration of the Fengshi fiber micro-bacteria is (1-9) multiplied by 109cfu/mL; the inoculation amount of the fiber fingi micro-bacteria is 10-20% of the volume of the LB liquid culture medium.
5. The method according to claim 4, wherein the temperature of the scale-up culture is 28 to 32 ℃; the rotation speed of the amplification culture is 150-200 rpm; the time of the amplification culture is 3-5 days.
6. Use of the fiber fense micro bacterium according to claim 1 or the microbial inoculum according to claim 2 or 3 for degrading diuron.
7. Use of the fiber fencing micro bacterium of claim 1 or the microbial inoculum of claim 2 or 3 to increase the rate of diuron degradation.
8. Use of the fiber fenugreek micro-bacterium of claim 1 or the inoculant of claim 2 or 3 for reducing diuron damage to plants and/or for promoting plant growth.
9. Use according to claim 6 or 7, wherein when said diuron is located in the soil, said use comprises the steps of:
inoculating a microbial inoculum containing the said F.finnii in the soil;
adjusting the water content of the soil to be 55-65%.
10. The use according to claim 9, wherein the means of inoculation comprises spraying or drip irrigation of the inoculum with water.
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